FastQCFastQC Report
Thu 26 May 2016
SRR938298_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938298_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences317328
Sequences flagged as poor quality0
Sequence length101
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36431.1480234961932134No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34391.0837367014571675No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA33291.0490722533151817No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24840.7827862653153834No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT17070.5379292088942672No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT12050.37973327282811475No Hit
ATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT11550.36397670549084854TruSeq Adapter, Index 11 (95% over 21bp)
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8940.2817274239903192No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8880.27983663590984725No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT8860.2792063732163566No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8350.26313467453234507No Hit
ACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCT6760.21302879039983863RNA PCR Primer, Index 11 (95% over 23bp)
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT5920.18655775727323148No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3960.12479201331114809No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC3870.12195583119044018No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC3860.12164069984369485No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC3780.11911964906973227No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3630.11439267886855242No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG3570.11250189078808048No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA3450.10872031462713658No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC3370.10619926385317399No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT3320.10462360711944738No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3200.1008420309585035No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATTCCG1003.0209776E-837.9970979
ATGGGCG404.4934056E-429.68523418-19
TAGGACG1703.597961E-927.9566654
CGAGCCT350.00838868627.14292384-85
ATAGCCC1109.4198695E-525.9234543
TACCATC950.00122514324.9980937
GTATAGC1351.32812565E-524.6549471
CCAAGAC1601.8435821E-623.7631663
AGGACGT2002.030174E-823.7631655
GACAGGG1801.9432082E-723.7481887
GGCATTC1601.8537266E-623.7481887
GGTATCA37900.023.3353081
TAGGCAT1852.516117E-723.120925
TATACAG2503.765308E-1022.8126415
CTATTAG1050.002179685522.6422981
GAGTACT29950.022.51914812-13
GGACGTG1703.1391664E-622.3512366
ACAGGGT2154.3995897E-822.091348
TTAGGCA1754.0146333E-621.7263224
TAGCCCT2205.574111E-821.6028794
Produced by FastQC (version 0.11.5)