##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938298_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 317328 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.597993243583925 31.0 31.0 34.0 30.0 34.0 2 31.832246130187063 31.0 31.0 34.0 30.0 34.0 3 31.84410452276509 31.0 31.0 34.0 30.0 34.0 4 35.57729856804316 37.0 35.0 37.0 33.0 37.0 5 35.45750768920486 37.0 35.0 37.0 33.0 37.0 6 35.497731054303436 37.0 35.0 37.0 33.0 37.0 7 35.434298265517064 37.0 35.0 37.0 33.0 37.0 8 35.45676082791307 37.0 35.0 37.0 33.0 37.0 9 36.95423347451218 39.0 37.0 39.0 33.0 39.0 10-11 36.89092358695105 39.0 37.0 39.0 32.5 39.0 12-13 36.78042908284173 39.0 37.0 39.0 32.0 39.0 14-15 37.68439438057783 39.5 36.5 41.0 32.0 41.0 16-17 37.628828845862955 39.0 36.5 41.0 32.0 41.0 18-19 37.80801725659255 39.5 37.0 41.0 32.5 41.0 20-21 37.90385500176474 40.0 37.0 41.0 33.0 41.0 22-23 37.767634750163865 40.0 37.0 41.0 32.0 41.0 24-25 37.634888821660866 39.5 36.0 41.0 32.0 41.0 26-27 37.39182486260273 39.0 36.0 41.0 32.0 41.0 28-29 37.19762989714113 39.0 36.0 41.0 31.5 41.0 30-31 36.83818005344628 39.0 35.5 40.0 31.0 41.0 32-33 36.44595497403317 38.0 35.0 40.0 30.5 41.0 34-35 36.1520965688499 38.0 35.0 40.0 30.0 41.0 36-37 35.96231974486966 38.0 35.0 40.0 30.0 41.0 38-39 35.71408605606817 38.0 35.0 40.0 29.5 41.0 40-41 35.45427444158725 38.0 35.0 40.0 28.5 41.0 42-43 35.0870786693894 38.0 34.5 40.0 26.5 41.0 44-45 34.58806345484798 38.0 34.0 40.0 24.5 41.0 46-47 33.90675421015479 38.0 33.0 40.0 21.0 41.0 48-49 33.31221323047446 38.0 33.0 40.0 12.0 41.0 50-51 32.82497605001765 38.0 33.0 40.0 7.0 41.0 52-53 32.32500283618212 37.0 32.0 40.0 2.0 41.0 54-55 31.957925238239298 37.0 31.0 40.0 2.0 41.0 56-57 31.54703020218827 37.0 31.0 40.0 2.0 41.0 58-59 31.040042164574196 36.0 30.0 39.0 2.0 41.0 60-61 30.59156929108052 36.0 29.0 39.0 2.0 40.0 62-63 30.082888998134422 35.0 28.0 39.0 2.0 40.0 64-65 29.539419780164373 35.0 28.0 38.5 2.0 40.0 66-67 28.99251247920133 35.0 26.5 38.0 2.0 40.0 68-69 28.43254928654263 34.0 26.0 38.0 2.0 40.0 70-71 27.868273521403722 34.0 25.5 37.0 2.0 39.0 72-73 27.46138065345636 33.5 24.5 36.5 2.0 39.0 74-75 27.007418191902385 33.0 23.5 36.0 2.0 39.0 76-77 25.800501373972672 31.5 22.0 34.5 2.0 36.5 78-79 26.43007235415721 33.0 23.0 35.0 2.0 37.0 80-81 26.154460053950487 33.0 21.5 35.0 2.0 37.0 82-83 25.706984571169265 32.5 20.0 35.0 2.0 36.0 84-85 25.2171428301316 32.0 18.5 35.0 2.0 36.0 86-87 24.704945671355823 31.5 14.5 34.5 2.0 35.5 88-89 24.13039032168608 31.0 7.0 34.0 2.0 35.0 90-91 23.639180910603542 31.0 2.0 34.0 2.0 35.0 92-93 22.964483121565067 30.5 2.0 34.0 2.0 35.0 94-95 22.309161183381235 30.0 2.0 34.0 2.0 35.0 96-97 21.414161372460043 29.0 2.0 34.0 2.0 35.0 98-99 20.323257008521153 29.0 2.0 34.0 2.0 35.0 100-101 17.176794988151062 22.5 2.0 31.0 2.0 34.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.44020952693350424 1101 2 0.44425468596492124 1101 3 0.5935279369016868 1101 4 0.19779399091039807 1101 5 0.2643324432368175 1101 6 0.007747319842508205 1101 7 0.3951398517545144 1101 8 0.3764319750094174 1101 9 0.3283413134108031 1101 10-11 0.32842054644515173 1101 12-13 0.24809275560194521 1101 14-15 0.5991516988715944 1101 16-17 0.6104272911535489 1101 18-19 0.6590567118151469 1101 20-21 0.6784036192777592 1101 22-23 0.7394003614424278 1101 24-25 0.6305334366435105 1101 26-27 0.4341366745432822 1101 28-29 0.47331048314621427 1101 30-31 0.8573811693391136 1101 32-33 0.5585854285321687 1101 34-35 0.5423080848029045 1101 36-37 0.7818871644948047 1101 38-39 0.6910829868537931 1101 40-41 0.7399634143205134 1101 42-43 1.0418224325260113 1101 44-45 0.9817390466960134 1101 46-47 1.4810937325190707 1101 48-49 0.6870709255004073 1101 50-51 0.9107601288653413 1101 52-53 1.0762578794419326 1101 54-55 0.8038843129469981 1101 56-57 0.6978262692960442 1101 58-59 0.6317840099418639 1101 60-61 0.28385965070390995 1101 62-63 0.6665190876555194 1101 64-65 0.5443061117674866 1101 66-67 0.7932776425443322 1101 68-69 0.7413643172297384 1101 70-71 0.988343540452032 1101 72-73 0.3815480537772835 1101 74-75 -0.2799243871146757 1101 76-77 -0.06504936703370845 1101 78-79 0.049451697328823485 1101 80-81 0.2760047421939298 1101 82-83 0.439058850439185 1101 84-85 0.5784360819190759 1101 86-87 0.3955154385383146 1101 88-89 0.28673511680586117 1101 90-91 0.5621789124169254 1101 92-93 0.23403709229980763 1101 94-95 0.2517342888595344 1101 96-97 0.174087502109316 1101 98-99 -0.02875806557205607 1101 100-101 9.918752907012163E-5 1102 1 0.3849778575433831 1102 2 0.4738050523135584 1102 3 0.4888418370650953 1102 4 0.10241683630599852 1102 5 0.29543254338968694 1102 6 0.3517038851263621 1102 7 0.5298823534676558 1102 8 0.4665379261980718 1102 9 0.5769839785312456 1102 10-11 0.5283309359870856 1102 12-13 0.5448246094841878 1102 14-15 0.6915295387324321 1102 16-17 0.6174116356794102 1102 18-19 0.5949377934660802 1102 20-21 0.6191974625121617 1102 22-23 0.6864722079449805 1102 24-25 0.6848004758085509 1102 26-27 0.7865851485298876 1102 28-29 0.801590489471657 1102 30-31 0.6500383829809948 1102 32-33 0.9647105669012603 1102 34-35 0.656603588466389 1102 36-37 0.5793938852791598 1102 38-39 0.9875091109379213 1102 40-41 0.9999918788189603 1102 42-43 0.3694435384244841 1102 44-45 0.8197655081371593 1102 46-47 0.9853581360824037 1102 48-49 0.8145549801100742 1102 50-51 0.6478694009121497 1102 52-53 0.34516305049248075 1102 54-55 0.1882710084295809 1102 56-57 0.37661494675110774 1102 58-59 0.4955603000689024 1102 60-61 0.6934268849034773 1102 62-63 0.30940032744256385 1102 64-65 1.1183823333688778 1102 66-67 1.0433698886041114 1102 68-69 1.0226093754765344 1102 70-71 0.3790609512369123 1102 72-73 0.6776315496393899 1102 74-75 0.36012621643811826 1102 76-77 0.6287780537664602 1102 78-79 0.7649543065745164 1102 80-81 0.9679292585610177 1102 82-83 1.131884742590362 1102 84-85 0.6492230725890487 1102 86-87 0.6823163451853027 1102 88-89 0.6441596068169275 1102 90-91 0.4387220572168822 1102 92-93 1.023130972432643 1102 94-95 0.4238812500107656 1102 96-97 0.758632333694365 1102 98-99 0.7638622496459071 1102 100-101 0.2772168935013468 1103 1 0.2568551763660416 1103 2 0.10261534170262365 1103 3 0.2965291979357332 1103 4 0.2299755798132992 1103 5 0.1368671216731343 1103 6 0.12744467167100737 1103 7 0.2321133700395066 1103 8 0.18076993213426107 1103 9 0.13272971189756078 1103 10-11 0.31018599411223846 1103 12-13 0.10587333567760737 1103 14-15 0.6856081931969342 1103 16-17 0.40142350805140836 1103 18-19 0.39043123892736986 1103 20-21 0.5080126987229008 1103 22-23 0.27211158464545093 1103 24-25 0.2308108641340496 1103 26-27 0.5270748838749313 1103 28-29 0.3563369648612209 1103 30-31 0.4226775123403783 1103 32-33 0.36632817758638936 1103 34-35 0.6109839990525856 1103 36-37 0.5744470636120838 1103 38-39 0.524298623675989 1103 40-41 0.663746516464272 1103 42-43 1.0494138574944856 1103 44-45 0.8609824262672561 1103 46-47 0.96341403516724 1103 48-49 1.2331995509732963 1103 50-51 1.5393856017531178 1103 52-53 1.5473297583826664 1103 54-55 1.3089536698196298 1103 56-57 1.0573470763578356 1103 58-59 0.9552140225522088 1103 60-61 1.4605305208174038 1103 62-63 1.4397095037967595 1103 64-65 1.3949492391319254 1103 66-67 1.8017770120273084 1103 68-69 1.6861562466118336 1103 70-71 1.394119076391501 1103 72-73 1.3987989995528203 1103 74-75 1.6242370252434597 1103 76-77 1.6778257905955485 1103 78-79 1.7671061739996965 1103 80-81 1.632297302093047 1103 82-83 1.3197650295060193 1103 84-85 1.40935663677406 1103 86-87 1.1908496125610135 1103 88-89 1.2058019516104004 1103 90-91 1.3253062768557662 1103 92-93 0.9443775714927476 1103 94-95 0.699300493147053 1103 96-97 0.4258151187549615 1103 98-99 0.6097539142513995 1103 100-101 0.9378797676047341 1104 1 0.10635350412523792 1104 2 0.21093832306907956 1104 3 0.05860277653105328 1104 4 0.12881934474401646 1104 5 0.0927104088207713 1104 6 0.11152493922953965 1104 7 0.014903623264544308 1104 8 0.14940825033779248 1104 9 0.14282049068398806 1104 10-11 0.20804074805395345 1104 12-13 0.16699612593082946 1104 14-15 0.1363678683044398 1104 16-17 0.2555754430729067 1104 18-19 0.3660977463330113 1104 20-21 0.4993027130563945 1104 22-23 0.09533662047918057 1104 24-25 -0.015734764150707292 1104 26-27 0.27310928425715986 1104 28-29 0.2836709352386677 1104 30-31 0.15928525242637903 1104 32-33 0.15443615656393916 1104 34-35 0.3739749211739394 1104 36-37 -0.050970996588588946 1104 38-39 -0.3261504924252989 1104 40-41 -0.14731893981857525 1104 42-43 -0.19025169434497968 1104 44-45 -0.5634752899295847 1104 46-47 -0.7973504177711206 1104 48-49 -0.796102885720444 1104 50-51 -0.9419330795655689 1104 52-53 -0.7085756405662167 1104 54-55 -0.5676206254503136 1104 56-57 -0.5427245911051344 1104 58-59 -0.5295590304817175 1104 60-61 -0.9522262875915786 1104 62-63 -1.0568695693040553 1104 64-65 -0.5311902735293259 1104 66-67 -0.5805641250964371 1104 68-69 -0.7304562713050338 1104 70-71 -1.015164248978781 1104 72-73 -0.653962586687026 1104 74-75 -0.6795374611397236 1104 76-77 -0.6751603536949453 1104 78-79 -0.8993773424838203 1104 80-81 -1.0983715942280234 1104 82-83 -0.7659253670539421 1104 84-85 -1.3026452743582873 1104 86-87 -1.2049936745866212 1104 88-89 -0.8177145319060841 1104 90-91 -1.0885542104829824 1104 92-93 -0.9355077605664981 1104 94-95 -0.6265571274931787 1104 96-97 -0.4504008392001282 1104 98-99 -0.3897396356769356 1104 100-101 0.16217025972131438 1105 1 0.3203398844290639 1105 2 0.39562738989539525 1105 3 0.3359731275001465 1105 4 0.237009870823492 1105 5 0.283984823248673 1105 6 0.16547988946526715 1105 7 0.2055980781025326 1105 8 0.2186105252760555 1105 9 0.14603833098931318 1105 10-11 0.4580126854931521 1105 12-13 0.5373283866507279 1105 14-15 0.30105768346570727 1105 16-17 0.3379109964666043 1105 18-19 0.08756747618409122 1105 20-21 0.30834634098425795 1105 22-23 0.2685480999373837 1105 24-25 0.4202825972202433 1105 26-27 0.32269582919467155 1105 28-29 0.4971896061957892 1105 30-31 0.4554949358806937 1105 32-33 0.6500677512657234 1105 34-35 0.7010627756976291 1105 36-37 0.3522505853915092 1105 38-39 0.07692142256384926 1105 40-41 -0.08410334451812673 1105 42-43 -0.0462626948688154 1105 44-45 0.22817890448783373 1105 46-47 -0.22909291571320267 1105 48-49 0.2523237400372409 1105 50-51 0.14068309943615276 1105 52-53 -0.04773975042179046 1105 54-55 0.022104735621297777 1105 56-57 0.1578104683874919 1105 58-59 0.38661810040206745 1105 60-61 0.32735165149395584 1105 62-63 0.17242961501084508 1105 64-65 -0.09298403241580289 1105 66-67 0.45066950507643 1105 68-69 0.28158256727911635 1105 70-71 0.4358104572997483 1105 72-73 0.8179000190152834 1105 74-75 0.46330097334355 1105 76-77 0.4319731492609762 1105 78-79 0.48573453359191276 1105 80-81 0.33503594898832034 1105 82-83 0.5719198024439258 1105 84-85 0.5643288332522793 1105 86-87 0.46517769411800813 1105 88-89 0.07729407394589671 1105 90-91 -0.04784478894590194 1105 92-93 0.49182891532963424 1105 94-95 0.5873357109413071 1105 96-97 0.6125528388661792 1105 98-99 0.17838690776391175 1105 100-101 -0.22933988021732432 1106 1 0.1460859455968091 1106 2 0.17338457247185346 1106 3 0.09960612101265909 1106 4 0.0730525028902207 1106 5 0.016867121673136865 1106 6 0.007444671671009928 1106 7 0.14288260080873982 1106 8 0.19000070136502956 1106 9 -0.1995779804101332 1106 10-11 0.0932629171891648 1106 12-13 -0.16489589509162528 1106 14-15 -0.01593026834152056 1106 16-17 -0.38473033810243606 1106 18-19 0.21350816200429534 1106 20-21 0.1018588525690518 1106 22-23 0.16749620003006527 1106 24-25 0.14158009490328283 1106 26-27 0.08861334541339261 1106 28-29 0.10095234947660714 1106 30-31 0.0503698200326852 1106 32-33 -0.04290259164437771 1106 34-35 -0.33670830863972157 1106 36-37 -1.2286298594648386 1106 38-39 -0.7957013763240184 1106 40-41 -0.22394579122804004 1106 42-43 -0.20135537327474395 1106 44-45 -0.36670988142505223 1106 46-47 -1.0304321186789096 1106 48-49 -0.5391081413343954 1106 50-51 -0.48984516747766094 1106 52-53 -0.7157471646942604 1106 54-55 -1.3048924840265208 1106 56-57 -1.1672683082575475 1106 58-59 -0.9601705928324087 1106 60-61 -1.1779310176441378 1106 62-63 -1.2310597269724717 1106 64-65 -1.5819738377911499 1106 66-67 -1.3474537572034606 1106 68-69 -1.7661514456958614 1106 70-71 -1.6274193851469612 1106 72-73 -1.6750471542933347 1106 74-75 -1.1726860516796194 1106 76-77 -1.1406357478659928 1106 78-79 -1.2482784413849224 1106 80-81 -1.903087313291568 1106 82-83 -1.0079272781862905 1106 84-85 -1.3829510555336348 1106 86-87 -1.2045350028236008 1106 88-89 -0.960351894543443 1106 90-91 -1.0377706462211584 1106 92-93 -1.4033147361995617 1106 94-95 -0.753007199160642 1106 96-97 -0.8126464197065744 1106 98-99 -0.7702460857485995 1106 100-101 -1.0128894631644982 1107 1 -0.2804751403760406 1107 2 0.08967416523203298 1107 3 -0.26799568894209 1107 4 -0.09690224824100113 1107 5 -0.1452143262906631 1107 6 -0.011876595297316328 1107 7 -0.13933459376139723 1107 8 -0.11484092759425124 1107 9 -0.10917074059112508 1107 10-11 0.10857965927061031 1107 12-13 -0.1791492887567827 1107 14-15 0.004069731658482567 1107 16-17 -0.11529594896216366 1107 18-19 -0.05904839908167503 1107 20-21 0.08602174849665545 1107 22-23 -0.01139520268486649 1107 24-25 0.16592398630599803 1107 26-27 -0.10120565911149271 1107 28-29 0.18131434042683026 1107 30-31 0.14785850781549215 1107 32-33 0.3255815712515542 1107 34-35 0.2803505148896903 1107 36-37 0.23304434867996093 1107 38-39 0.013190026390915932 1107 40-41 -0.030914117019889886 1107 42-43 0.035771323557831636 1107 44-45 0.6017969059052746 1107 46-47 0.10115159172833188 1107 48-49 0.13519050119953846 1107 50-51 0.2568064162327488 1107 52-53 0.21647002987587172 1107 54-55 0.6274378327155574 1107 56-57 -0.07907826300868237 1107 58-59 -0.15071357925775075 1107 60-61 -0.04082694524594288 1107 62-63 0.2617457028917798 1107 64-65 0.3540216373219707 1107 66-67 0.13874533781916654 1107 68-69 0.12511552262992964 1107 70-71 0.5880783524095996 1107 72-73 0.28834651086503627 1107 74-75 -0.27112496570676825 1107 76-77 -0.38156334967594674 1107 78-79 -0.05483952735777109 1107 80-81 0.15840589937811345 1107 82-83 0.031416613216425304 1107 84-85 0.1838362747831077 1107 86-87 0.43587223699540445 1107 88-89 -0.01666411173801663 1107 90-91 0.10417505513630942 1107 92-93 0.09030517330270271 1107 94-95 0.21733216735519534 1107 96-97 0.10289656671876202 1107 98-99 0.3073557242061504 1107 100-101 -0.4756722685943622 1108 1 0.18356268714434876 1108 2 0.12491622665837454 1108 3 0.2262954788524567 1108 4 0.034314132116449514 1108 5 0.062195009124920375 1108 6 0.18129536375179356 1108 7 0.1388798778679643 1108 8 0.09854619716715973 1108 9 0.3062899569622317 1108 10-11 0.11595862855744343 1108 12-13 0.28134871575475273 1108 14-15 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2316 88-89 -0.9124350288004948 2316 90-91 -0.7779952042718001 2316 92-93 -0.6560046549764351 2316 94-95 -1.256991910101874 2316 96-97 -1.1072331373367703 2316 98-99 -0.8043359089688593 2316 100-101 -0.44031421232837786 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 3.0 4 12.0 5 37.0 6 70.0 7 171.0 8 381.0 9 601.0 10 1041.0 11 1506.0 12 2092.0 13 2810.0 14 3576.0 15 4693.0 16 5897.0 17 7352.0 18 6442.0 19 4742.0 20 4051.0 21 4164.0 22 4220.0 23 4479.0 24 4871.0 25 5113.0 26 5497.0 27 6421.0 28 7457.0 29 9181.0 30 10859.0 31 12404.0 32 14967.0 33 18227.0 34 23134.0 35 30055.0 36 37774.0 37 47717.0 38 24449.0 39 860.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.125085479647176 22.515260283556078 16.116813255726743 20.24284098107 2 15.69811249373406 23.845254121333834 38.930801945830744 21.52583143910136 3 19.32246119908611 27.389584810525484 32.720397069250765 20.567556921137633 4 12.400103363081733 21.21180608077447 36.45754550496647 29.930545051177333 5 11.327014965385647 40.13751123586646 34.63106933909451 13.904404459653383 6 26.786794736045984 39.17397771391116 17.655863964100238 16.38336358594262 7 23.2715045631019 37.292958705188326 21.04919830585388 18.386338425855897 8 24.3464175868502 35.12895174708819 20.561059849745376 19.96357081631624 9 24.293475520596985 17.99935713205264 22.208566530529925 35.49860081682045 10-11 23.11346619270912 28.675849594110826 27.517111632128273 20.693572581051782 12-13 25.288502747945348 27.646473049967224 27.566902384914034 19.4981218171734 14-15 21.714125447486513 29.159576211364897 28.132248020975144 20.99405032017345 16-17 18.487338022487773 31.793444007462313 29.32423234003933 20.39498563001059 18-19 17.805551353804265 29.569719659153936 33.53832627439117 19.08640271265063 20-21 19.73966999445369 27.982560631271113 33.36169515454041 18.916074219734785 22-23 18.776155901779862 29.228117279282007 33.109590077144155 18.88613674179398 24-25 18.99683764198704 29.313026173357727 33.591584692737605 18.09855149191763 26-27 18.68558020948398 29.13220308001645 34.196124530615016 17.986092179884558 28-29 17.430702616850706 29.988844350325216 33.96722003731155 18.61323299551253 30-31 18.690597741138507 29.109785458579136 34.06475318912923 18.13486361115313 32-33 17.887800635304796 29.8079589572934 33.93444637725004 18.369794030151766 34-35 18.36084431802961 29.836037157557183 33.87703968672933 17.926078837683875 36-37 18.50454183769134 29.254004143983742 33.633391370114474 18.608062648210442 38-39 18.014033307439103 29.382020789968376 33.8485392344867 18.755406668105817 40-41 18.422773476954397 29.33522208898686 33.67505314094918 18.56695129310956 42-43 18.168611292749603 29.288983778588367 33.29241871568017 19.249986212981856 44-45 18.65246684818232 29.36393888972924 32.669194020067565 19.314400242020874 46-47 19.4053156355569 29.043135178742503 32.04996092371301 19.501588261987596 48-49 18.96019260827913 28.879897141128424 32.195551605909344 19.964358644683102 50-51 18.364594362930468 29.543721323047446 31.973068875107145 20.11861543891494 52-53 18.920643624262592 29.81347375586144 31.347218020470933 19.91866459940503 54-55 18.65293954520244 29.22669918822165 31.110239247718447 21.010122018857462 56-57 19.492449452931982 29.643302828618967 30.171147834417383 20.693099884031664 58-59 19.17322139867897 29.805910603539555 30.46910452276509 20.551763475016386 60-61 19.062137598951242 30.039738062824583 30.495102858871576 20.403021479352596 62-63 18.585028739978824 30.000346644481418 30.53654263096859 20.87808198457117 64-65 18.41249432763576 30.376140775475218 30.02760550597489 21.183759390914133 66-67 18.709062403983268 30.019459391322844 29.58300178837321 21.68847641632068 68-69 18.637861242986283 29.343936569662915 29.862175785355372 22.156026401995437 70-71 18.979888317450712 28.95773458377452 29.883905611859024 22.178471486915747 72-73 18.77914964957394 28.445961276660114 30.430658498462158 22.344230575303786 74-75 18.399889073765944 29.170290677154238 29.587524580245045 22.84229566883477 76-77 18.159916553219382 28.453524428982004 30.428137447688197 22.95842157011042 78-79 18.14132380376141 28.46707507689205 30.144361669944033 23.24723944940251 80-81 18.30989244397664 28.098971710213256 30.050295755429012 23.540840090381092 82-83 18.08351059639171 28.15914283463326 30.34286614669503 23.414480422279997 84-85 18.06660321702299 28.051097972972972 29.91535145219847 23.96694735780557 86-87 17.84011962744057 28.289557652642376 29.64088132270889 24.229441397208166 88-89 18.065591645211406 27.95554743214413 29.332518191014316 24.646342731630146 90-91 18.15299671618649 27.420000378174304 29.676913089242834 24.75008981639638 92-93 18.112216358963774 27.58075723746512 29.648356808251492 24.65866959531962 94-95 17.914457461140305 27.34005089379269 29.678486736888544 25.067004908178458 96-97 17.662639288055264 27.427929460999344 29.61683179549236 25.292599455453036 98-99 17.95382065244794 27.285332526597085 29.02942696515908 25.731419855795895 100-101 17.757651389098978 26.928288710734634 29.511105228659307 25.802954671507084 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2382.0 1 1397.0 2 449.5 3 466.0 4 373.0 5 353.5 6 615.0 7 1074.5 8 1321.0 9 1270.0 10 975.5 11 643.5 12 566.5 13 600.0 14 612.0 15 607.5 16 666.0 17 749.0 18 851.5 19 984.0 20 1071.5 21 1227.5 22 1480.0 23 1726.5 24 1982.5 25 2361.5 26 3025.0 27 3789.0 28 4322.5 29 4840.0 30 5547.0 31 6207.5 32 6818.0 33 7481.5 34 8680.0 35 9955.5 36 10733.0 37 11296.5 38 11110.0 39 10711.5 40 10728.0 41 10659.5 42 10637.5 43 10987.0 44 11383.5 45 11549.5 46 11744.0 47 12654.5 48 14383.0 49 14060.0 50 11980.0 51 10393.5 52 8705.5 53 7185.5 54 6040.0 55 5104.5 56 4258.5 57 3472.0 58 2799.0 59 2171.5 60 1617.0 61 1183.5 62 850.5 63 609.5 64 444.5 65 327.0 66 236.5 67 179.5 68 151.0 69 128.0 70 108.0 71 88.0 72 78.0 73 68.0 74 52.0 75 48.5 76 44.0 77 27.5 78 19.5 79 18.5 80 11.5 81 5.5 82 1.5 83 0.5 84 0.0 85 0.5 86 0.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 3.1513134674532344E-4 2 0.04317299450410931 3 9.453940402359704E-4 4 0.0 5 0.08287954419402006 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 4.726970201179852E-4 26-27 0.007720717995260425 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.009611506075732365 36-37 1.5756567337266172E-4 38-39 0.00362401048757122 40-41 0.00362401048757122 42-43 1.5756567337266172E-4 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 1.5756567337266172E-4 68-69 0.0011029597136086322 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0015756567337266172 82-83 9.453940402359704E-4 84-85 0.005042101547925175 86-87 0.0029937477940805727 88-89 3.1513134674532344E-4 90-91 0.004411838854434529 92-93 0.0011029597136086322 94-95 1.5756567337266172E-4 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 317328.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.17079650632531 #Duplication Level Percentage of deduplicated Percentage of total 1 77.91688856989069 46.10404357975027 2 13.195891966762407 15.616228765695025 3 4.352651036419591 7.726494862170886 4 1.8899087056912862 4.473096137599669 5 0.9176259395140459 2.714832886795559 6 0.511222524791754 1.8149666390341632 7 0.2931812778395383 1.214343881135542 8 0.1749381635439745 0.8280984381000618 9 0.10643595246039926 0.5668110075592079 >10 0.5469493871537028 5.863263892375422 >50 0.05221488497965497 2.127723026159143 >100 0.03516512661895125 4.098444116175555 >500 0.0031968296926319323 1.5041289906364286 >1k 0.0037296346414039208 5.347523776813059 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3643 1.1480234961932134 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3439 1.0837367014571675 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3329 1.0490722533151817 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2484 0.7827862653153834 No Hit CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT 1707 0.5379292088942672 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1205 0.37973327282811475 No Hit ATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT 1155 0.36397670549084854 TruSeq Adapter, Index 11 (95% over 21bp) GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 894 0.2817274239903192 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 888 0.27983663590984725 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 886 0.2792063732163566 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 835 0.26313467453234507 No Hit ACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCT 676 0.21302879039983863 RNA PCR Primer, Index 11 (95% over 23bp) GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 592 0.18655775727323148 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 396 0.12479201331114809 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 387 0.12195583119044018 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 386 0.12164069984369485 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 378 0.11911964906973227 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 363 0.11439267886855242 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 357 0.11250189078808048 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 345 0.10872031462713658 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 337 0.10619926385317399 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 332 0.10462360711944738 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 320 0.1008420309585035 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 1.5756567337266172E-4 0.0 12-13 0.0 0.0 0.0 3.1513134674532344E-4 0.0 14-15 0.0 0.0 0.0 3.1513134674532344E-4 0.0 16-17 0.0 0.0 0.0 3.1513134674532344E-4 0.0 18-19 0.0 0.0 0.0 6.302626934906469E-4 0.0 20-21 0.0 0.0 0.0 0.0026786164473352496 0.0 22-23 0.0 0.0 0.0 0.007563152321887763 0.0 24-25 0.0 0.0 0.0 0.01733222407099279 0.0 26-27 0.0 0.0 0.0 0.03119800332778702 0.0 28-29 0.0 0.0 0.0 0.06617758281651792 0.0 30-31 0.0 0.0 0.0 0.1399183179549236 0.0 32-33 0.0 0.0 0.0 0.26691625069328895 0.0 34-35 0.0 0.0 0.0 0.47821181868602836 0.0 36-37 0.0 0.0 0.0 0.7641935158574094 0.0 38-39 0.0 0.0 0.0 1.0890939343518378 0.0 40-41 0.0 0.0 0.0 1.4117884334190491 0.0 42-43 0.0 0.0 0.0 1.713684263601069 0.0 44-45 0.0 0.0 0.0 2.08254550496647 0.0 46-47 0.0 0.0 0.0 2.4898527706348004 0.0 48-49 0.0 0.0 0.0 2.876203801744567 0.0 50-51 0.0 0.0 0.0 3.2625548328543337 0.0 52-53 0.0 0.0 0.0 3.624325618917965 0.0 54-55 0.0 0.0 0.0 4.054795038572077 0.0 56-57 0.0 0.0 0.0 4.555696314223768 0.0 58-59 0.0 0.0 0.0 5.065578833257701 0.0 60-61 0.0 0.0 0.0 5.605556395905813 0.0 62-63 0.0 0.0 0.0 6.122844501588261 0.0 64-65 0.0 0.0 0.0 6.66912469117128 0.0 66-67 0.0 0.0 0.0 7.291824232340039 0.0 68-69 0.0 0.0 0.0 7.990627993747793 0.0 70-71 0.0 0.0 0.0 8.726144557051379 0.0 72-73 0.0 0.0 0.0 9.413130892956184 0.0 74-75 0.0 0.0 0.0 10.114770836484647 0.0 76-77 0.0 0.0 0.0 10.876915998588212 0.0 78-79 0.0 0.0 0.0 11.727613069127212 0.0 80-81 0.0 0.0 0.0 12.608562748953764 0.0 82-83 0.0 0.0 0.0 13.429164775878586 0.0 84-85 0.0 0.0 0.0 14.282067765844804 0.0 86-87 0.0 0.0 0.0 15.180349669742348 0.0 88-89 0.0 0.0 0.0 16.138979226541622 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CATTCCG 100 3.0209776E-8 37.997097 9 ATGGGCG 40 4.4934056E-4 29.685234 18-19 TAGGACG 170 3.597961E-9 27.956665 4 CGAGCCT 35 0.008388686 27.142923 84-85 ATAGCCC 110 9.4198695E-5 25.923454 3 TACCATC 95 0.001225143 24.998093 7 GTATAGC 135 1.32812565E-5 24.654947 1 CCAAGAC 160 1.8435821E-6 23.763166 3 AGGACGT 200 2.030174E-8 23.763165 5 GACAGGG 180 1.9432082E-7 23.748188 7 GGCATTC 160 1.8537266E-6 23.748188 7 GGTATCA 3790 0.0 23.335308 1 TAGGCAT 185 2.516117E-7 23.12092 5 TATACAG 250 3.765308E-10 22.812641 5 CTATTAG 105 0.0021796855 22.642298 1 GAGTACT 2995 0.0 22.519148 12-13 GGACGTG 170 3.1391664E-6 22.351236 6 ACAGGGT 215 4.3995897E-8 22.09134 8 TTAGGCA 175 4.0146333E-6 21.726322 4 TAGCCCT 220 5.574111E-8 21.602879 4 >>END_MODULE