FastQCFastQC Report
Thu 26 May 2016
SRR938297_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938297_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences319596
Sequences flagged as poor quality0
Sequence length101
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41411.295698319127899No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36111.1298639532409667No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA32571.019099112629695No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24890.7787957296086309No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT12200.38173193656991955No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10370.3244721460844316No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9360.29286974805692184No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT8750.2737831512284259No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8480.2653349854190916No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT6050.18930149313508304No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4870.15237987959799246No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4110.1285998573198663No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC3900.1220290616903841No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG3820.11952590145058135No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC3640.11389379091102517No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA3580.1120164207311731No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC3520.11013905055132103No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3510.1098261555213457No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT3340.10450694001176485No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTCC250.005017474357.001418
CGAGACA804.4734805E-429.7021834
GTACTAG700.00826703727.220231
TCGAGAC700.00836245927.1562823
TTCGAGA700.00836245927.1562822
GTCCTAC1457.6647666E-726.28161
GGTATCA33650.026.0472931
CATTCCG1109.45405E-525.909739
TGTAGTG1309.953275E-625.5895715
GTATCAA45050.024.5314371
TAGAAGG1201.7013354E-423.7617454
ATTCGTA500.001652606423.75058732-33
AATTCGT500.001652606423.75058732-33
TAGCCCT3450.022.039884
GAGTACT30200.021.62380412-13
TAGACTA1100.002864634621.6015874
ATAGATC1100.002864634621.6015873
TTCGTAC550.002875569721.59144234-35
TCGTACA550.002875569721.59144234-35
TATCAAC51450.021.4294472
Produced by FastQC (version 0.11.5)