##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938297_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 319596 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.225672411419417 31.0 31.0 34.0 30.0 34.0 2 31.357204095170154 33.0 31.0 34.0 30.0 34.0 3 31.36877495337864 33.0 31.0 34.0 28.0 34.0 4 35.02717180440306 37.0 35.0 37.0 33.0 37.0 5 35.05730359578968 37.0 35.0 37.0 33.0 37.0 6 35.112272994655754 37.0 35.0 37.0 33.0 37.0 7 35.066784315197935 37.0 35.0 37.0 32.0 37.0 8 35.05025407076434 37.0 35.0 37.0 32.0 37.0 9 36.54914329340793 39.0 37.0 39.0 32.0 39.0 10-11 36.45553292281505 39.0 37.0 39.0 32.0 39.0 12-13 36.33420631046697 39.0 37.0 39.0 32.0 39.0 14-15 37.33792193894792 40.0 37.0 41.0 32.0 41.0 16-17 37.20167023367001 39.5 37.0 41.0 32.0 41.0 18-19 37.19959104619582 39.5 37.0 41.0 32.0 41.0 20-21 37.232540457327374 40.0 37.0 41.0 32.0 41.0 22-23 37.25173343846606 40.0 37.0 41.0 32.0 41.0 24-25 37.17816555901826 40.0 37.0 41.0 32.0 41.0 26-27 36.968488341531184 39.5 36.0 41.0 31.5 41.0 28-29 36.78415718594726 39.0 36.0 41.0 31.0 41.0 30-31 36.451939323395784 39.0 35.5 40.5 30.5 41.0 32-33 36.02713269252432 38.0 35.0 40.0 30.0 41.0 34-35 35.717934517328125 38.0 35.0 40.0 30.0 41.0 36-37 35.4162536452271 38.0 35.0 40.0 29.0 41.0 38-39 35.06037309603374 38.0 35.0 40.0 27.0 41.0 40-41 34.80922007784828 38.0 35.0 40.0 25.5 41.0 42-43 34.45414210440681 38.0 34.5 40.0 23.5 41.0 44-45 33.94359128399604 38.0 33.5 40.0 19.5 41.0 46-47 33.303246911726056 38.0 33.0 40.0 9.5 41.0 48-49 32.736062090889746 38.0 33.0 40.0 2.0 41.0 50-51 31.902059162192266 37.5 32.0 39.5 2.0 40.5 52-53 31.899372958359926 37.5 32.0 39.5 2.0 40.5 54-55 31.97216329365824 38.0 32.0 40.0 2.0 41.0 56-57 31.67904479405249 37.5 31.5 40.0 2.0 41.0 58-59 31.31180146184558 37.0 31.0 40.0 2.0 41.0 60-61 30.90191992390393 36.5 30.5 40.0 2.0 41.0 62-63 30.411574300053818 36.0 29.0 39.0 2.0 41.0 64-65 29.9943287775817 36.0 28.0 39.0 2.0 40.5 66-67 29.487851850461208 35.0 28.0 39.0 2.0 40.0 68-69 28.9295250879235 35.0 26.5 38.0 2.0 40.0 70-71 28.35254508817382 34.5 26.0 38.0 2.0 40.0 72-73 27.773287212605915 34.0 25.5 37.0 2.0 39.0 74-75 27.143427952790397 34.0 23.0 36.5 2.0 39.0 76-77 26.48171754339854 33.5 21.0 36.0 2.0 38.5 78-79 25.809324897683325 33.0 18.5 35.0 2.0 37.5 80-81 25.128862689145045 32.0 10.5 35.0 2.0 37.0 82-83 24.497318489593113 32.0 4.5 35.0 2.0 36.5 84-85 23.83361180991001 31.0 2.0 35.0 2.0 36.0 86-87 23.163368753050726 31.0 2.0 34.5 2.0 35.5 88-89 22.449795992440457 30.5 2.0 34.0 2.0 35.0 90-91 21.682162793026194 29.5 2.0 34.0 2.0 35.0 92-93 20.882673750610145 29.0 2.0 34.0 2.0 35.0 94-95 20.069622273119812 28.5 2.0 34.0 2.0 35.0 96-97 19.052192455475037 26.5 2.0 34.0 2.0 35.0 98-99 17.91661503898672 24.0 2.0 34.0 2.0 35.0 100-101 15.166636941638817 2.0 2.0 31.0 2.0 34.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.5191071685402662 1101 2 0.4037888784806327 1101 3 0.25611138826389634 1101 4 0.14891778594609661 1101 5 0.3597429858521437 1101 6 0.23071342329556188 1101 7 0.3391837484775735 1101 8 0.23738947794964105 1101 9 0.5024022232149434 1101 10-11 0.6429398079785713 1101 12-13 0.40149153790041225 1101 14-15 0.803082824443706 1101 16-17 0.8306520338671035 1101 18-19 0.6835005586742113 1101 20-21 0.692335263301949 1101 22-23 0.37056852073782665 1101 24-25 0.5487553848026892 1101 26-27 0.8006755752938233 1101 28-29 0.8759645442868802 1101 30-31 0.697482200093333 1101 32-33 0.892041975336916 1101 34-35 0.8747443456931379 1101 36-37 1.1167550073180053 1101 38-39 0.9887260480110385 1101 40-41 1.0969622710356788 1101 42-43 1.009399105696069 1101 44-45 0.8532450486763423 1101 46-47 0.7232108099854955 1101 48-49 0.4797797227735856 1101 50-51 0.9394953581071412 1101 52-53 0.7794335901891607 1101 54-55 0.7676191240755799 1101 56-57 0.6706325733465484 1101 58-59 0.9873333652368252 1101 60-61 1.219191597554623 1101 62-63 1.156538168413551 1101 64-65 0.840310529164725 1101 66-67 0.9402482342870258 1101 68-69 1.3596187845671466 1101 70-71 1.2019926368217497 1101 72-73 0.712825701248601 1101 74-75 0.9784687397027199 1101 76-77 1.396540920440593 1101 78-79 1.5849994644286092 1101 80-81 2.186302417675819 1101 82-83 2.4381433150227387 1101 84-85 2.32940444159086 1101 86-87 2.884766565950077 1101 88-89 3.262572965166349 1101 90-91 3.0323469972791486 1101 92-93 3.1628173220575206 1101 94-95 3.701700554693595 1101 96-97 3.9881698840245967 1101 98-99 4.5006146263017754 1101 100-101 4.811936037686294 1102 1 0.7151879415290843 1102 2 0.7898055976207914 1102 3 0.7154969055801494 1102 4 0.7767974949896299 1102 5 0.7096398482992186 1102 6 0.6861471438470801 1102 7 0.7384563573148313 1102 8 0.7754092369334629 1102 9 0.862829972644974 1102 10-11 0.9747170308642481 1102 12-13 0.8119192873304399 1102 14-15 1.2065189334438173 1102 16-17 1.1599648120670807 1102 18-19 1.0928535062369136 1102 20-21 1.260167203373598 1102 22-23 1.0351651986229697 1102 24-25 1.145145570450282 1102 26-27 1.111510446643301 1102 28-29 1.151564370581525 1102 30-31 1.0434272657756694 1102 32-33 1.2076917419203497 1102 34-35 1.183863876688683 1102 36-37 0.9279047196185175 1102 38-39 0.6244822091227462 1102 40-41 1.0537259231907115 1102 42-43 0.7930870874570104 1102 44-45 0.8484952929494938 1102 46-47 1.0035875334682913 1102 48-49 0.47295635940369607 1102 50-51 0.37391073103081496 1102 52-53 0.05806783185673581 1102 54-55 0.225587857112167 1102 56-57 0.05528136283737339 1102 58-59 -0.06800807434628098 1102 60-61 0.45572400445941597 1102 62-63 0.5198537693255041 1102 64-65 0.853381856925008 1102 66-67 0.791523882967951 1102 68-69 0.8466071680217127 1102 70-71 0.8518515012230097 1102 72-73 0.855122954532721 1102 74-75 1.3114456151776963 1102 76-77 1.6368459904655595 1102 78-79 1.5499924076486735 1102 80-81 2.0175530962123496 1102 82-83 1.8433707604683995 1102 84-85 1.8620393346535025 1102 86-87 2.3447383388303287 1102 88-89 2.6061621520081673 1102 90-91 2.928769752680296 1102 92-93 2.7263446263675846 1102 94-95 3.0398180229380856 1102 96-97 3.1812542396863073 1102 98-99 3.5703138989106122 1102 100-101 4.420491026286882 1103 1 0.5286440102057135 1103 2 0.6235853980034776 1103 3 0.6111994447537548 1103 4 0.6805157553195684 1103 5 0.44678625156755913 1103 6 0.582563340775252 1103 7 0.733160809918644 1103 8 0.7152447853639359 1103 9 0.7694442354572004 1103 10-11 0.5623728202835068 1103 12-13 0.8948742441489799 1103 14-15 0.7375940743653615 1103 16-17 0.8234027807281947 1103 18-19 0.9004193127218869 1103 20-21 1.0281198330472847 1103 22-23 1.0377922865890454 1103 24-25 1.062935299984332 1103 26-27 1.0010641519750436 1103 28-29 1.2396545603731184 1103 30-31 1.2245049924137206 1103 32-33 1.0903570984906565 1103 34-35 1.3444834143625783 1103 36-37 1.4448411627068936 1103 38-39 1.31215868427401 1103 40-41 1.2627811023808704 1103 42-43 1.5634185763125714 1103 44-45 1.298849536110275 1103 46-47 1.5042029339957708 1103 48-49 1.940645245994176 1103 50-51 1.9047428146011676 1103 52-53 1.790213459410129 1103 54-55 2.0417589281159394 1103 56-57 1.7135013555973693 1103 58-59 1.916684483961621 1103 60-61 2.0558222616864974 1103 62-63 1.9895233147565428 1103 64-65 1.829060607506797 1103 66-67 1.87619575554384 1103 68-69 2.134562900555199 1103 70-71 2.517559728972916 1103 72-73 2.1213534970160417 1103 74-75 2.1229158535807606 1103 76-77 2.3869675191411552 1103 78-79 2.478104317458879 1103 80-81 2.5905840730960428 1103 82-83 2.3796949058890995 1103 84-85 2.5968120501729715 1103 86-87 2.6196465458945077 1103 88-89 2.846085614799705 1103 90-91 3.3858003158493517 1103 92-93 3.615117445315718 1103 94-95 3.5491110880464305 1103 96-97 3.653638787444489 1103 98-99 3.902164754782774 1103 100-101 4.1259624024052 1104 1 0.5619222636732424 1104 2 0.3218378640792885 1104 3 0.3730040614479613 1104 4 0.26589455457849454 1104 5 0.3802097155903965 1104 6 0.25377660000112456 1104 7 0.24301009137676033 1104 8 0.3614618000352081 1104 9 0.41255674860125424 1104 10-11 0.43711619818144953 1104 12-13 0.4537095553502155 1104 14-15 0.8460713664388635 1104 16-17 1.0615781349918834 1104 18-19 0.8804153069134628 1104 20-21 0.6171539324914832 1104 22-23 0.6045641057267872 1104 24-25 0.4785829891335993 1104 26-27 0.5069827339188606 1104 28-29 0.42226434456018325 1104 30-31 0.6511035603372122 1104 32-33 0.5688158637002161 1104 34-35 0.7145950762723459 1104 36-37 0.7947535356476578 1104 38-39 0.8953643324638918 1104 40-41 1.1203145258448899 1104 42-43 1.2986296323438182 1104 44-45 1.2020992481927948 1104 46-47 1.6218284910535061 1104 48-49 1.4876584150893635 1104 50-51 1.3764868434429935 1104 52-53 1.2882005406779662 1104 54-55 1.5404720621603047 1104 56-57 1.7861166477710206 1104 58-59 1.8355568488907785 1104 60-61 2.152367251922339 1104 62-63 2.325370292874812 1104 64-65 2.550181232412914 1104 66-67 2.3754947390699535 1104 68-69 2.393913959590801 1104 70-71 2.6011359144418478 1104 72-73 2.6082845470385756 1104 74-75 2.548578063785552 1104 76-77 2.941842087264938 1104 78-79 3.5465836124365993 1104 80-81 3.620597592699472 1104 82-83 3.8235685226333764 1104 84-85 3.590142379149988 1104 86-87 3.8326904595693385 1104 88-89 4.532235532179911 1104 90-91 4.644850939253288 1104 92-93 4.672220244374351 1104 94-95 5.192849508756456 1104 96-97 5.372380963599916 1104 98-99 5.67242162724785 1104 100-101 6.32455035493637 1105 1 0.7029817396724773 1105 2 0.7813521115453455 1105 3 0.7263699057776627 1105 4 0.7604633057737473 1105 5 0.6163256077303814 1105 6 0.6315352536633938 1105 7 0.6818989802656503 1105 8 0.7282336493371773 1105 9 0.7653679935811795 1105 10-11 0.9499293555729267 1105 12-13 0.7804211636883309 1105 14-15 0.8692880426675913 1105 16-17 1.2610856890175057 1105 18-19 1.124845408271284 1105 20-21 0.9224178452855156 1105 22-23 0.8269536639597206 1105 24-25 0.9372796806616179 1105 26-27 1.0628633994380792 1105 28-29 1.3430713822346974 1105 30-31 1.1240859661509361 1105 32-33 1.0011738671425547 1105 34-35 1.5503906394775484 1105 36-37 1.505329171524501 1105 38-39 1.7675100582240688 1105 40-41 1.4331946176406447 1105 42-43 1.690870022475771 1105 44-45 1.1891283168483682 1105 46-47 1.4706475769207827 1105 48-49 1.0767337641044676 1105 50-51 1.0162028503276765 1105 52-53 1.0470071958701688 1105 54-55 0.8694403161285607 1105 56-57 1.106412115355642 1105 58-59 1.323805079910091 1105 60-61 1.8379381067702916 1105 62-63 2.0303712490805914 1105 64-65 2.0612971245528975 1105 66-67 2.305882958614795 1105 68-69 2.220506042206978 1105 70-71 2.536539433279099 1105 72-73 2.6541107088285933 1105 74-75 2.469671963192706 1105 76-77 2.766813401091671 1105 78-79 2.9170129488297896 1105 80-81 2.9779125668819155 1105 82-83 3.263274967460301 1105 84-85 3.334032224244652 1105 86-87 3.633001226446371 1105 88-89 3.7533772418758353 1105 90-91 3.7494837562355023 1105 92-93 4.171125388761425 1105 94-95 4.307900187662561 1105 96-97 4.130962910434874 1105 98-99 4.633394088521513 1105 100-101 4.9808899992278235 1106 1 0.528390517641494 1106 2 0.3333458005872245 1106 3 0.6180437439876414 1106 4 0.6827596339435686 1106 5 0.6052097155903979 1106 6 0.42599882222334884 1106 7 0.6938037421704095 1106 8 0.2932078317812383 1106 9 0.6369615105060191 1106 10-11 0.42273127754653217 1106 12-13 0.34408653947720325 1106 14-15 0.7387300965975996 1106 16-17 0.6991773413410911 1106 18-19 0.7385502275483873 1106 20-21 0.6079277420152849 1106 22-23 0.7697426771553637 1106 24-25 0.9674718780224936 1106 26-27 0.6760303529664782 1106 28-29 0.6676016461474887 1106 30-31 0.9518972111308628 1106 32-33 1.3417325303668761 1106 34-35 1.2051704730977448 1106 36-37 1.1372138531079798 1106 38-39 1.5167929038924584 1106 40-41 1.8247788115591774 1106 42-43 1.4626177275819146 1106 44-45 1.7676746450181895 1106 46-47 2.2951419831169986 1106 48-49 2.406606827787776 1106 50-51 1.843153510109662 1106 52-53 2.300700540677969 1106 54-55 2.080749839938086 1106 56-57 2.334628552532916 1106 58-59 1.2746441504780712 1106 60-61 2.3455220138270967 1106 62-63 2.7320171182716457 1106 64-65 2.6171455181271988 1106 66-67 2.7574391835143928 1106 68-69 2.4445091976860382 1106 70-71 2.742703374759305 1106 72-73 3.114236927990955 1106 74-75 3.2254629844204743 1106 76-77 3.2204135158363627 1106 78-79 3.37862726323025 1106 80-81 3.659585687937561 1106 82-83 3.8297193162841694 1106 84-85 3.915142379149991 1106 86-87 4.279515856394735 1106 88-89 3.9441402940846686 1106 90-91 4.374017605919953 1106 92-93 4.22410516500927 1106 94-95 3.9519764928834427 1106 96-97 4.192817471536426 1106 98-99 4.461707341533561 1106 100-101 4.735264640650659 1107 1 -0.3985216188945344 1107 2 -0.6609125222109213 1107 3 -0.39957635715015627 1107 4 -0.4178250689641416 1107 5 -0.524636470671723 1107 6 -0.5819025684214054 1107 7 -0.24804202268420283 1107 8 -0.4946134914383151 1107 9 -0.865909328094908 1107 10-11 -0.7133965142781662 1107 12-13 -0.4321592464477817 1107 14-15 -0.6322401941735762 1107 16-17 -0.6066196455952806 1107 18-19 -0.4954099494427595 1107 20-21 -0.5648098264634314 1107 22-23 -0.6653473986979819 1107 24-25 -0.7784193988422317 1107 26-27 -0.48568477556280953 1107 28-29 -0.8141593104475362 1107 30-31 -0.6963328773647177 1107 32-33 -0.5522966521025694 1107 34-35 -0.512262628461464 1107 36-37 -0.6965935636640381 1107 38-39 -0.4085126165458064 1107 40-41 -0.6364912052971192 1107 42-43 -0.533945167487623 1107 44-45 -0.8095877865030872 1107 46-47 -0.4429137480250134 1107 48-49 -0.8230734292707993 1107 50-51 -0.5645582471596207 1107 52-53 -0.7128108373245574 1107 54-55 -0.6766511292991666 1107 56-57 -0.580339315145121 1107 58-59 -0.31603747615487876 1107 60-61 -0.14927623733177242 1107 62-63 -0.319355089903663 1107 64-65 -0.39124312494065094 1107 66-67 -0.016344002326309948 1107 68-69 -0.0325504314753573 1107 70-71 0.04973824623002088 1107 72-73 0.8006570712695016 1107 74-75 0.5287578643193385 1107 76-77 0.8821286443656504 1107 78-79 0.6778660959314706 1107 80-81 1.1127172724297694 1107 82-83 1.2498362568657129 1107 84-85 0.9924179796556771 1107 86-87 0.9759971037609354 1107 88-89 1.1498925064740533 1107 90-91 1.4444716725023383 1107 92-93 1.8485652366485468 1107 94-95 1.5002803276917014 1107 96-97 1.5151678718735475 1107 98-99 2.080807636518813 1107 100-101 2.225940999689847 1108 1 -0.2740844197018646 1108 2 0.06695064603417578 1108 3 -0.04554230446682794 1108 4 -0.08389658994949656 1108 5 -0.2223800922625685 1108 6 -0.5107179697565982 1108 7 -0.5476331834770392 1108 8 -0.5463911657124925 1108 9 -0.5266245379484502 1108 10-11 -0.20970919864394233 1108 12-13 -0.1611609542571273 1108 14-15 -0.19114667867391688 1108 16-17 0.021310799987709572 1108 18-19 -0.18871585432296456 1108 20-21 -0.4099555077758552 1108 22-23 -0.6468978825772993 1108 24-25 -0.3489441620777569 1108 26-27 -0.4281885275681887 1108 28-29 -0.35033381751166104 1108 30-31 -0.3770501572901921 1108 32-33 -0.4511089483298676 1108 34-35 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0.8852578737137726 2316 76-77 1.1184268613019412 2316 78-79 1.3152268234364968 2316 80-81 1.2695056909706217 2316 82-83 1.6097276572001533 2316 84-85 1.6122533891590862 2316 86-87 1.4951664478415019 2316 88-89 2.1039067484371117 2316 90-91 2.5359640724046386 2316 92-93 2.933528504408727 2316 94-95 2.9835582338600872 2316 96-97 2.958115469716585 2316 98-99 3.080337914781971 2316 100-101 3.0091879042236016 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3905.0 3 754.0 4 518.0 5 430.0 6 444.0 7 517.0 8 618.0 9 688.0 10 869.0 11 1290.0 12 2019.0 13 2787.0 14 3423.0 15 4673.0 16 5793.0 17 7273.0 18 5997.0 19 3995.0 20 3938.0 21 4026.0 22 4099.0 23 4415.0 24 4767.0 25 5523.0 26 6098.0 27 7163.0 28 8197.0 29 9533.0 30 10979.0 31 13006.0 32 15784.0 33 18624.0 34 23167.0 35 30051.0 36 34239.0 37 40521.0 38 27177.0 39 2296.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.058144278138634 21.436819479745207 15.721861369983369 19.783174872132793 2 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18.95600011265173 29.229524766169433 32.703265961341685 19.111209159837156 46-47 19.263014598882787 29.269899388192588 32.198281932122235 19.26880408080239 48-49 19.043036746700466 28.99210614844196 32.354777383997934 19.610079720859638 50-51 18.693959036412252 29.609968192279467 31.9019076809465 19.794165090361776 52-53 18.975500695442932 29.851633625956524 31.4003826891816 19.77248298941894 54-55 18.887517269110234 29.016329576764033 31.40858049869592 20.687572655429815 56-57 19.502628449840415 29.24432067088053 30.864259340384255 20.3887915388948 58-59 19.742717580756704 28.89764715822149 30.86606732150289 20.49356793951892 60-61 19.133157161028684 28.698249385509122 31.741993625879495 20.4265998275827 62-63 19.143100764278394 28.501873538131782 31.734059280467484 20.62096641712234 64-65 18.87552023030948 29.04793941859374 31.15295553399881 20.923584817097975 66-67 19.23051048457626 28.841150416864185 30.61139559151847 21.316943507041085 68-69 19.09854289259211 28.42853633693868 30.9501942444805 21.522726525988706 70-71 19.46637208436278 28.10715744245396 31.084483263104033 21.34198721007922 72-73 19.38904040569995 27.62320976759251 31.51121980681998 21.47653001988756 74-75 19.07005344916071 28.429289389027275 30.754390466772648 21.746266695039367 76-77 19.180754938392333 27.785957363583023 30.873303344416193 22.15998435360845 78-79 19.29688966768884 27.70057575567933 30.751298579385445 22.251235997246386 80-81 19.486848919422545 27.392830153845193 30.657483622754633 22.462837303977633 82-83 19.518778075581693 27.446516554716382 30.864012091569226 22.170693278132692 84-85 19.60501777310504 27.155301892460198 30.689427505757482 22.55025282867728 86-87 19.549487392499486 27.40341967793261 30.344987272815455 22.702105656752448 88-89 20.19926203881338 26.971573785165038 30.08819230805342 22.740971867968167 90-91 20.403446036353962 26.567970015414833 30.150470660959787 22.878113287271418 92-93 20.590614570245204 26.541980325365323 30.370507137414194 22.49689796697528 94-95 20.48726138072518 26.135568766323285 30.387408632622947 22.989761220328585 96-97 20.460424089643745 26.28410657478214 30.001423959766093 23.25404537580802 98-99 21.100453763104365 26.00328587075575 29.38616804881865 23.51009231732123 100-101 21.658770370486387 25.366610022161833 29.897804960179176 23.076814647172604 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2563.0 1 1475.5 2 455.0 3 484.5 4 355.0 5 361.0 6 611.0 7 1021.0 8 1361.5 9 1407.5 10 1107.0 11 770.5 12 690.0 13 684.5 14 704.0 15 729.5 16 792.0 17 862.0 18 971.0 19 1121.5 20 1245.5 21 1429.5 22 1640.0 23 1863.0 24 2143.5 25 2537.5 26 3090.5 27 3826.0 28 4409.5 29 4913.0 30 5627.5 31 6237.5 32 6725.0 33 7363.0 34 8573.0 35 9834.5 36 10704.0 37 11243.5 38 10876.0 39 10371.0 40 10336.5 41 10308.0 42 10382.0 43 10520.0 44 10773.0 45 11024.5 46 11241.0 47 12161.5 48 13519.0 49 13243.0 50 11576.0 51 10115.0 52 8845.5 53 7678.5 54 6676.0 55 5797.0 56 4860.0 57 3957.0 58 3187.0 59 2525.0 60 1956.5 61 1459.5 62 1060.5 63 805.5 64 642.5 65 534.5 66 430.5 67 343.5 68 308.0 69 253.5 70 202.5 71 173.5 72 154.5 73 138.0 74 117.0 75 98.0 76 68.0 77 60.0 78 52.5 79 36.0 80 25.5 81 16.5 82 13.5 83 9.0 84 4.0 85 2.5 86 2.5 87 1.0 88 0.5 89 0.5 90 0.0 91 0.0 92 0.5 93 0.5 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2834828971576615 2 0.03911187874691798 3 0.04568267437640021 4 0.048185834616202954 5 0.06038874078524136 6 0.058198475575413966 7 0.031602398027509734 8 0.03254108311743576 9 0.040050563836844015 10-11 0.03316687317738645 12-13 0.03410555826731248 14-15 0.028004105182793275 16-17 0.02080751949336037 18-19 0.027534762637830258 20-21 0.03989411632185634 22-23 0.03238463560244809 24-25 0.054287287700722164 26-27 0.047716492071239944 28-29 0.0384860886869673 30-31 0.038955431231930314 32-33 0.03441845329728783 34-35 0.0328539781474111 36-37 0.03191529305748508 38-39 0.019868834403434336 40-41 0.019868834403434336 42-43 0.020494624463385024 44-45 0.008448165809334284 46-47 0.01595764652874254 48-49 0.013610933803927458 50-51 0.006101453084519206 52-53 0.00484987296461783 54-55 0.0070401381744452365 56-57 0.005006320479605502 58-59 0.007822375749383596 60-61 0.0070401381744452365 62-63 0.004224082904667142 64-65 0.00813527077935894 66-67 0.002346712724815079 68-69 0.007665928234395924 70-71 0.014862513923828835 72-73 0.015488303983779523 74-75 0.012515801199013755 76-77 0.010481983504174019 78-79 0.005006320479605502 80-81 0.006727243144469893 82-83 0.010794878534149364 84-85 0.003754740359704126 86-87 0.0014080276348890474 88-89 0.020963967008348038 90-91 0.03082016045257137 92-93 0.014862513923828835 94-95 0.023623574763138463 96-97 0.020181729433409682 98-99 0.014393171378865817 100-101 0.11060839309628406 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 319596.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.415186245885856 #Duplication Level Percentage of deduplicated Percentage of total 1 79.2301853491235 48.659365895124765 2 12.481635129681948 15.331238922852169 3 4.109971755218842 7.572440424362867 4 1.7301948933407838 4.250409664648194 5 0.845998088950101 2.5978565098266975 6 0.4554846466923554 1.6784204645251513 7 0.2796694426736861 1.202316563636127 8 0.16531326451298634 0.8122195943184357 9 0.12513814113621213 0.6916844019909978 >10 0.4912058333321872 5.46349297372281 >50 0.04897923181073696 2.02793961071999 >100 0.031122220213072167 3.7531923141538748 >500 0.0020408013254473553 1.0227433389045122 >1k 0.0030612019881710332 4.936679321213417 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4141 1.295698319127899 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3611 1.1298639532409667 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3257 1.019099112629695 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2489 0.7787957296086309 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1220 0.38173193656991955 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1037 0.3244721460844316 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 936 0.29286974805692184 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 875 0.2737831512284259 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 848 0.2653349854190916 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 605 0.18930149313508304 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 487 0.15237987959799246 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 411 0.1285998573198663 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 390 0.1220290616903841 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 382 0.11952590145058135 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 364 0.11389379091102517 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 358 0.1120164207311731 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 352 0.11013905055132103 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 351 0.1098261555213457 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 334 0.10450694001176485 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 4.6934254496301583E-4 0.0 22-23 0.0 0.0 0.0 0.004224082904667142 0.0 24-25 0.0 1.5644751498767193E-4 0.0 0.012359353684026082 0.0 26-27 0.0 3.1289502997534387E-4 0.0 0.02518804991301518 0.0 28-29 0.0 3.1289502997534387E-4 0.0 0.054287287700722164 0.0 30-31 0.0 3.1289502997534387E-4 0.0 0.12328064181028547 0.0 32-33 0.0 3.1289502997534387E-4 0.0 0.24671773113555867 0.0 34-35 0.0 3.1289502997534387E-4 0.0 0.4386788320254321 0.0 36-37 0.0 3.1289502997534387E-4 0.0 0.7359291105020088 0.0 38-39 0.0 3.1289502997534387E-4 0.0 1.0669720522159225 0.0 40-41 0.0 3.1289502997534387E-4 0.0 1.3659432533573637 0.0 42-43 0.0 3.1289502997534387E-4 0.0 1.6689820898884844 0.0 44-45 0.0 3.1289502997534387E-4 0.0 2.0408578330141802 0.0 46-47 0.0 3.1289502997534387E-4 0.0 2.460293620696129 0.0 48-49 0.0 3.1289502997534387E-4 0.0 2.856888071189877 0.0 50-51 0.0 3.1289502997534387E-4 0.0 3.2387764552747846 0.0 52-53 0.0 3.1289502997534387E-4 0.0 3.626296949899248 0.0 54-55 0.0 3.1289502997534387E-4 0.0 4.069512759859322 0.0 56-57 0.0 3.1289502997534387E-4 0.0 4.591421669858196 0.0 58-59 0.0 3.1289502997534387E-4 0.0 5.074375148625139 0.0 60-61 0.0 3.1289502997534387E-4 0.0 5.614119075332607 0.0 62-63 0.0 3.1289502997534387E-4 0.0 6.121165471407652 0.0 64-65 0.0 3.1289502997534387E-4 0.0 6.646829121766229 0.0 66-67 0.0 3.1289502997534387E-4 0.0 7.266204833602423 0.0 68-69 3.1289502997534387E-4 3.1289502997534387E-4 0.0 7.9807006345511216 0.0 70-71 3.1289502997534387E-4 3.1289502997534387E-4 0.0 8.714283032328314 0.0 72-73 3.1289502997534387E-4 3.1289502997534387E-4 0.0 9.407814866268664 0.0 74-75 4.693425449630158E-4 3.1289502997534387E-4 0.0 10.095401694639483 0.0 76-77 6.257900599506877E-4 3.1289502997534387E-4 0.0 10.868565313708558 0.0 78-79 6.257900599506877E-4 3.1289502997534387E-4 0.0 11.70696754652749 0.0 80-81 6.257900599506877E-4 3.1289502997534387E-4 0.0 12.559606503210304 0.0 82-83 6.257900599506877E-4 3.1289502997534387E-4 0.0 13.39112504536978 0.0 84-85 6.257900599506877E-4 3.1289502997534387E-4 0.0 14.238131891513035 0.0 86-87 6.257900599506877E-4 3.1289502997534387E-4 0.0 15.145996820986497 0.0 88-89 7.822375749383597E-4 3.1289502997534387E-4 0.0 16.086872176122355 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCCGTCC 25 0.0050174743 57.00141 8 CGAGACA 80 4.4734805E-4 29.702183 4 GTACTAG 70 0.008267037 27.22023 1 TCGAGAC 70 0.008362459 27.156282 3 TTCGAGA 70 0.008362459 27.156282 2 GTCCTAC 145 7.6647666E-7 26.2816 1 GGTATCA 3365 0.0 26.047293 1 CATTCCG 110 9.45405E-5 25.90973 9 TGTAGTG 130 9.953275E-6 25.589571 5 GTATCAA 4505 0.0 24.531437 1 TAGAAGG 120 1.7013354E-4 23.761745 4 ATTCGTA 50 0.0016526064 23.750587 32-33 AATTCGT 50 0.0016526064 23.750587 32-33 TAGCCCT 345 0.0 22.03988 4 GAGTACT 3020 0.0 21.623804 12-13 TAGACTA 110 0.0028646346 21.601587 4 ATAGATC 110 0.0028646346 21.601587 3 TTCGTAC 55 0.0028755697 21.591442 34-35 TCGTACA 55 0.0028755697 21.591442 34-35 TATCAAC 5145 0.0 21.429447 2 >>END_MODULE