FastQCFastQC Report
Thu 26 May 2016
SRR938297_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938297_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences319596
Sequences flagged as poor quality0
Sequence length101
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37621.1771111027672436No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34771.0879360192242706No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA34491.079174958384961No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24490.7662799284096171No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT17780.5563273632961614No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT11810.3695290304008811No Hit
ATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT11740.3673387651910537TruSeq Adapter, Index 11 (95% over 21bp)
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8830.27628631146822863No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8430.2637705102692149No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT8240.25782550469968335No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8020.25094181404022575No Hit
ACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCT6680.2090138800235297RNA PCR Primer, Index 11 (95% over 23bp)
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT5140.16082804540732673No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4250.13298038773952114No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC4010.12547090702011288No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC4000.12515801199013754No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC3820.11952590145058135No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA3800.11890011139063068No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG3590.11232931576114845No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT3380.10575852013166623No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3280.10262956983191279No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC3220.10075219965206073No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTATAG1404.456524E-1033.9781045
CAGGCCG650.005840897629.2276389
TAGGACG1903.8562575E-1027.540154
GTATAGC1457.8650373E-726.204096
GGTATCA34950.025.7403321
TAGGACC1309.879057E-625.6142644
AGGACGT2059.349606E-1025.5250155
CACCGCA500.001653875323.74745886-87
GACGTGA1050.002194142422.6166277
GTAGGAC1703.0977062E-622.3855743
GAGTACT29850.022.35522712-13
GGACGTG2154.4014087E-822.0906586
GGCCATA1100.002838293821.6359941
ATGGGCA2205.5213604E-821.622435
GTACTTT29900.021.60303914-15
TCACCGC550.002875158321.59197684-85
GTATCAA44400.021.333871
TACATGG18900.021.1551932
ATATAGG1353.734672E-421.1551931
TGGAGTG1353.7505274E-421.141935
Produced by FastQC (version 0.11.5)