##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938297_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 319596 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.60415336862789 31.0 31.0 34.0 30.0 34.0 2 31.8337651284747 31.0 31.0 34.0 30.0 34.0 3 31.853142717681074 31.0 31.0 34.0 30.0 34.0 4 35.591409154057 37.0 35.0 37.0 33.0 37.0 5 35.464630345811585 37.0 35.0 37.0 33.0 37.0 6 35.522027810110266 37.0 35.0 37.0 33.0 37.0 7 35.45519968960813 37.0 35.0 37.0 33.0 37.0 8 35.474686792075 37.0 35.0 37.0 33.0 37.0 9 36.955740998009986 39.0 37.0 39.0 33.0 39.0 10-11 36.904333909060185 39.0 37.0 39.0 32.5 39.0 12-13 36.816135058010744 39.0 37.0 39.0 32.0 39.0 14-15 37.71108211617167 39.5 37.0 41.0 32.0 41.0 16-17 37.63993917320617 39.0 36.5 41.0 32.0 41.0 18-19 37.756714727343265 39.0 37.0 41.0 32.0 41.0 20-21 37.819008998861065 39.5 37.0 41.0 32.5 41.0 22-23 37.766958910624666 40.0 37.0 41.0 32.0 41.0 24-25 37.65150220903891 39.5 36.0 41.0 32.0 41.0 26-27 37.41879591734565 39.0 36.0 41.0 32.0 41.0 28-29 37.2378455925606 39.0 36.0 41.0 31.5 41.0 30-31 36.86585251379867 39.0 35.5 40.0 31.0 41.0 32-33 36.48079919648556 38.0 35.0 40.0 30.5 41.0 34-35 36.15608612122805 38.0 35.0 40.0 30.0 41.0 36-37 35.999439917896346 38.0 35.0 40.0 30.0 41.0 38-39 35.73561152204658 38.0 35.0 40.0 30.0 41.0 40-41 35.480517590958584 38.0 35.0 40.0 28.5 41.0 42-43 35.10285641872865 38.0 34.5 40.0 26.5 41.0 44-45 34.58486026107961 38.0 34.0 40.0 24.5 41.0 46-47 33.91964229840173 38.0 33.0 40.0 21.0 41.0 48-49 33.29535726354523 38.0 33.0 40.0 12.0 41.0 50-51 32.80310454448741 38.0 33.0 40.0 7.0 41.0 52-53 32.26558217249277 37.0 31.5 40.0 2.0 41.0 54-55 31.929130840185735 37.0 31.0 40.0 2.0 41.0 56-57 31.500863590282734 36.5 31.0 40.0 2.0 41.0 58-59 30.991063717943902 36.0 30.0 39.0 2.0 41.0 60-61 30.513984843364746 36.0 29.0 39.0 2.0 40.0 62-63 29.975018460806766 35.0 28.0 39.0 2.0 40.0 64-65 29.450556327363294 35.0 27.5 38.5 2.0 40.0 66-67 28.87699939924154 34.5 26.0 38.0 2.0 40.0 68-69 28.286270791874742 34.0 26.0 37.5 2.0 40.0 70-71 27.72411262969499 34.0 24.5 37.0 2.0 39.0 72-73 27.169554687793337 33.0 23.5 36.0 2.0 39.0 74-75 26.607158725390804 33.0 22.0 36.0 2.0 38.5 76-77 25.387249840423536 31.0 20.5 34.5 2.0 36.5 78-79 25.97727756292319 32.0 21.0 35.0 2.0 37.0 80-81 25.683600545688932 32.0 20.0 35.0 2.0 37.0 82-83 25.23704458128387 32.0 18.0 35.0 2.0 36.0 84-85 24.693849422395775 31.5 12.5 35.0 2.0 36.0 86-87 24.120478666816858 31.0 7.0 34.0 2.0 35.0 88-89 23.433458178450294 31.0 2.0 34.0 2.0 35.0 90-91 22.91246605088925 30.5 2.0 34.0 2.0 35.0 92-93 22.21751680246311 30.0 2.0 34.0 2.0 35.0 94-95 21.504145859147172 29.0 2.0 34.0 2.0 35.0 96-97 20.600899260316147 29.0 2.0 34.0 2.0 35.0 98-99 19.531699395486804 28.0 2.0 34.0 2.0 35.0 100-101 16.77766148512497 20.5 2.0 31.0 2.0 34.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.16622743795860018 1101 2 0.4785989991062074 1101 3 0.4533763576611811 1101 4 0.3300063493486576 1101 5 0.26794674954288666 1101 6 0.47440475117620196 1101 7 0.17037649894783868 1101 8 0.4801352533968739 1101 9 0.5802708008423849 1101 10-11 0.8204916590948059 1101 12-13 0.5775379388041557 1101 14-15 0.7325966977266489 1101 16-17 0.5990273705159268 1101 18-19 0.8048608828928252 1101 20-21 0.807629583001578 1101 22-23 1.1355699783596478 1101 24-25 1.0445316687996566 1101 26-27 0.8383770697892672 1101 28-29 0.7035158493310405 1101 30-31 0.9613707859432878 1101 32-33 0.8447324462041337 1101 34-35 1.152849046306649 1101 36-37 1.023770483828038 1101 38-39 1.3262098610623028 1101 40-41 0.7907519552206992 1101 42-43 0.9723224198900908 1101 44-45 0.9313153763106143 1101 46-47 0.9008586602075113 1101 48-49 0.8248342047862423 1101 50-51 0.9048132494178631 1101 52-53 0.8905769763074503 1101 54-55 1.1290668725280497 1101 56-57 0.9528826914074813 1101 58-59 0.8682526128997239 1101 60-61 1.0569966574113572 1101 62-63 1.158576654520573 1101 64-65 1.409998196322153 1101 66-67 1.061020505001796 1101 68-69 1.1123074569196127 1101 70-71 1.7476701275623334 1101 72-73 1.4429696140637311 1101 74-75 1.630596684566271 1101 76-77 1.498821445132517 1101 78-79 2.027555429821266 1101 80-81 1.797592267207321 1101 82-83 1.7380562181238055 1101 84-85 1.3142606456148194 1101 86-87 1.464002293151733 1101 88-89 1.6064018570831422 1101 90-91 2.025017759688094 1101 92-93 1.7890391859432455 1101 94-95 2.319886525554086 1101 96-97 2.803608487204343 1101 98-99 3.298829150490061 1101 100-101 3.33405971317017 1102 1 0.3815895050522933 1102 2 0.4755374422719889 1102 3 0.2961838704176678 1102 4 0.33728026097605834 1102 5 0.05724215720763226 1102 6 0.2121096692089921 1102 7 0.18366495839849506 1102 8 0.22819735306031674 1102 9 0.2504369065855201 1102 10-11 0.29206369253308395 1102 12-13 0.6393010481299584 1102 14-15 0.6265930064879086 1102 16-17 0.6785192823604618 1102 18-19 0.7094043635138192 1102 20-21 0.7245224285123797 1102 22-23 1.1250716611302423 1102 24-25 0.767493345056316 1102 26-27 0.6241332309009806 1102 28-29 0.5042812385396047 1102 30-31 0.3500147985369182 1102 32-33 0.2958995290398718 1102 34-35 0.6157683818836759 1102 36-37 0.2793941946086278 1102 38-39 0.21262284553738908 1102 40-41 0.5321806817433341 1102 42-43 0.05314969163040928 1102 44-45 0.19438670407090086 1102 46-47 -0.18180337431642357 1102 48-49 0.5161923635095178 1102 50-51 0.7101764021700063 1102 52-53 0.8174144532372054 1102 54-55 0.805687217767435 1102 56-57 0.6373794887150552 1102 58-59 0.8669063964194272 1102 60-61 0.6811579862573005 1102 62-63 0.9478232075549897 1102 64-65 1.1479962421369372 1102 66-67 0.9198414799230861 1102 68-69 1.135274561468524 1102 70-71 1.312397084461697 1102 72-73 1.2850660205342557 1102 74-75 1.4504370927738464 1102 76-77 1.3139012410287272 1102 78-79 1.2034320990211356 1102 80-81 1.3866162602591068 1102 82-83 1.51937203616745 1102 84-85 1.8921023566125434 1102 86-87 1.631698525916903 1102 88-89 1.3792929655404187 1102 90-91 1.2462803804675957 1102 92-93 1.7613603237133013 1102 94-95 1.7476413838756564 1102 96-97 2.6604969900813416 1102 98-99 2.9925486163461024 1102 100-101 3.982371514277542 1103 1 0.17388406987620542 1103 2 0.28405996386272037 1103 3 0.2113620262846574 1103 4 0.28298021443315946 1103 5 0.046102890516834805 1103 6 0.07061926221721393 1103 7 0.035565460027491724 1103 8 -0.07256284759128562 1103 9 0.315297019322756 1103 10-11 0.03231295560388503 1103 12-13 0.19012072026110616 1103 14-15 0.2904653755213715 1103 16-17 0.20857047954719832 1103 18-19 0.3614186366689083 1103 20-21 0.10340746755669983 1103 22-23 -0.011149055630369276 1103 24-25 0.20073531640657194 1103 26-27 0.3077087596800112 1103 28-29 0.23874366807882552 1103 30-31 0.021882202994696343 1103 32-33 0.20602519486187276 1103 34-35 0.280457837331781 1103 36-37 0.37811167922361477 1103 38-39 0.38545213653392807 1103 40-41 0.054722958208145656 1103 42-43 0.2665230173789723 1103 44-45 0.2284250761037825 1103 46-47 0.41486622282894103 1103 48-49 0.45518496836031375 1103 50-51 0.7413394574526251 1103 52-53 0.9061200435562853 1103 54-55 0.6049218456828314 1103 56-57 0.3357142872877361 1103 58-59 0.5668236534913618 1103 60-61 0.463450999651311 1103 62-63 0.29927379451263647 1103 64-65 0.48711813281284577 1103 66-67 0.5711214095915977 1103 68-69 0.7843100892132604 1103 70-71 0.6111042262106743 1103 72-73 0.5051053234250844 1103 74-75 0.6755702571736997 1103 76-77 0.4199466462105015 1103 78-79 0.6165520245524974 1103 80-81 0.7889537479769544 1103 82-83 0.930506131425247 1103 84-85 1.338578025020258 1103 86-87 1.5578090911545317 1103 88-89 1.8119505649612186 1103 90-91 1.5673694842582577 1103 92-93 1.660615637360717 1103 94-95 1.7105053234733205 1103 96-97 2.3675042818018746 1103 98-99 2.1440095461697553 1103 100-101 3.020733204301848 1104 1 0.33756140200779683 1104 2 0.541943895563687 1104 3 0.43721951965003925 1104 4 0.3552349838488098 1104 5 0.0685354176812254 1104 6 0.40456348133493236 1104 7 0.5785127335741365 1104 8 0.49184554457735885 1104 9 0.7455709164736248 1104 10-11 0.6176166459548895 1104 12-13 0.7425016726420637 1104 14-15 0.7444436440106799 1104 16-17 0.7489590429641524 1104 18-19 0.820749666662401 1104 20-21 0.9123704639014036 1104 22-23 0.6523680441643336 1104 24-25 0.6916671675907864 1104 26-27 1.0489836082103778 1104 28-29 1.2130764506134994 1104 30-31 1.1544644472488628 1104 32-33 1.0848144392662604 1104 34-35 1.1210767347330162 1104 36-37 1.3677366354100826 1104 38-39 1.689307727140303 1104 40-41 1.4709264532759931 1104 42-43 1.410396634123245 1104 44-45 1.540392428765145 1104 46-47 1.3476788010673815 1104 48-49 1.5240247860960352 1104 50-51 1.6595909220763332 1104 52-53 1.8206410990868065 1104 54-55 1.7934051464689702 1104 56-57 1.579898354184735 1104 58-59 1.7171015564506575 1104 60-61 1.5705412805586256 1104 62-63 1.4589603400035962 1104 64-65 1.2334503144762543 1104 66-67 1.2824227966027628 1104 68-69 1.363169435265032 1104 70-71 1.5345932853464213 1104 72-73 1.7878503036464046 1104 74-75 1.638181042812878 1104 76-77 1.7874895834695295 1104 78-79 1.973429496887384 1104 80-81 2.0832855290595234 1104 82-83 2.157545338275181 1104 84-85 2.288485390700494 1104 86-87 2.371849449674386 1104 88-89 2.4185851851605094 1104 90-91 2.1946540468740494 1104 92-93 2.6194659703435406 1104 94-95 2.7955987088821637 1104 96-97 3.4571142162067616 1104 98-99 4.019038337917792 1104 100-101 4.709251555911681 1105 1 0.37663196999402615 1105 2 0.3179431305990654 1105 3 0.4731441706349351 1105 4 0.33658897122689524 1105 5 0.19370275369193024 1105 6 0.35994691985089844 1105 7 0.5816108402867002 1105 8 0.4397418918713001 1105 9 0.610411230109122 1105 10-11 0.6105359421874468 1105 12-13 0.49132553953820945 1105 14-15 0.7873820643587379 1105 16-17 0.6440584883648057 1105 18-19 0.781841653658006 1105 20-21 0.7083018083267234 1105 22-23 0.624967967667871 1105 24-25 0.42716152597671453 1105 26-27 0.5298116824119461 1105 28-29 0.7978966839277106 1105 30-31 0.8962028293486952 1105 32-33 1.0256348638216224 1105 34-35 1.0079814966377754 1105 36-37 1.2365786702159767 1105 38-39 1.3444616762701571 1105 40-41 0.9017688705641902 1105 42-43 1.1516091030465603 1105 44-45 0.7471814897710729 1105 46-47 0.6188731020809612 1105 48-49 0.8689377713704687 1105 50-51 0.6965530563276232 1105 52-53 0.5874272914754073 1105 54-55 0.662777875480252 1105 56-57 0.5002177269137462 1105 58-59 1.0230539374030343 1105 60-61 1.468719708556332 1105 62-63 1.3805705905295085 1105 64-65 1.4654019304640187 1105 66-67 2.0321168107546086 1105 68-69 1.7931799535285649 1105 70-71 1.777541267752234 1105 72-73 1.8071082879646312 1105 74-75 1.9740100732202244 1105 76-77 1.8406689676730075 1105 78-79 2.147807963133321 1105 80-81 2.4030837696408973 1105 82-83 2.2245418959343866 1105 84-85 2.249252267732434 1105 86-87 2.709404431506478 1105 88-89 2.929648485982849 1105 90-91 2.690231595162441 1105 92-93 2.816386987746487 1105 94-95 3.157694711942021 1105 96-97 3.759963709417704 1105 98-99 4.147757978384419 1105 100-101 4.253791620933676 1106 1 0.02863283057922672 1106 2 0.2655550066747985 1106 3 0.1507115831421011 1106 4 0.009996888610714905 1106 5 0.10226557641138356 1106 6 0.039880941652391755 1106 7 0.15529844785984892 1106 8 0.2652582429900576 1106 9 0.038229646632359504 1106 10-11 0.5404341062723503 1106 12-13 0.16333500597539086 1106 14-15 0.33501904083607315 1106 16-17 0.3488598366149489 1106 18-19 0.5048766507893916 1106 20-21 0.6024498289807667 1106 22-23 0.31972915527544643 1106 24-25 0.3447425644161868 1106 26-27 0.5167415447183146 1106 28-29 0.6488899426769876 1106 30-31 0.6967263520107707 1106 32-33 0.7520763440281613 1106 34-35 0.32643387759015496 1106 36-37 0.4973001274735793 1106 38-39 0.5624228065053813 1106 40-41 0.5874939135934554 1106 42-43 1.0915474277740458 1106 44-45 1.0474360795587998 1106 46-47 0.757301816940398 1106 48-49 1.2007112940325442 1106 50-51 1.2625671125525173 1106 52-53 1.7276847498804528 1106 54-55 1.8592781623419867 1106 56-57 2.0820808938672783 1106 58-59 1.932280127879224 1106 60-61 1.8735174710348126 1106 62-63 2.0920952606385157 1106 64-65 1.9621209493968905 1106 66-67 2.118533907713875 1106 68-69 2.122891657487255 1106 70-71 2.7791369361400733 1106 72-73 2.6885447480908518 1106 74-75 2.836692947574786 1106 76-77 2.7923506945806373 1106 78-79 2.8101358460937362 1106 80-81 2.9421148941388857 1106 82-83 3.164390576370419 1106 84-85 3.286203644668749 1106 86-87 3.510143100468035 1106 88-89 3.883069312144638 1106 90-91 3.4143961103661127 1106 92-93 2.796450097327668 1106 94-95 3.8544280739615253 1106 96-97 4.274772946365495 1106 98-99 4.377570083949536 1106 100-101 5.468973778133904 1107 1 -0.03302435441866791 1107 2 0.16144101594576554 1107 3 0.15355608377421603 1107 4 -0.11337015732269862 1107 5 -0.18318532961474432 1107 6 -0.1598135379093435 1107 7 0.04349377023658718 1107 8 -0.04210585309085246 1107 9 0.2329989260255303 1107 10-11 0.12353039788634135 1107 12-13 -0.032593144045677036 1107 14-15 0.2564397108740053 1107 16-17 -0.02430967455234878 1107 18-19 -0.025253985535933055 1107 20-21 -0.13422315879841307 1107 22-23 -0.04680318775026393 1107 24-25 0.09873276669178921 1107 26-27 0.05249533738582102 1107 28-29 0.12203838346923845 1107 30-31 -0.1090838039100106 1107 32-33 -0.3072630997139356 1107 34-35 -0.29990935881944836 1107 36-37 -0.14858588179738774 1107 38-39 -0.27932550786461263 1107 40-41 0.014471895051521244 1107 42-43 -0.08752442220488632 1107 44-45 0.2993192443291335 1107 46-47 0.4600119939315519 1107 48-49 0.10848889622386082 1107 50-51 0.24800221579735648 1107 52-53 -0.07858632049459402 1107 54-55 0.12824149988939837 1107 56-57 -0.29584262067128364 1107 58-59 -0.8231850006500636 1107 60-61 -0.2815868273216928 1107 62-63 -0.07185859524011562 1107 64-65 -0.038076585369228866 1107 66-67 -0.3895255107437734 1107 68-69 -0.31376552751063613 1107 70-71 0.10258036218305477 1107 72-73 0.24194497986497865 1107 74-75 -0.06763438070165861 1107 76-77 -0.3338476830004815 1107 78-79 -0.36303629887044764 1107 80-81 0.165474038681662 1107 82-83 -0.1438031657281904 1107 84-85 0.25176675044203023 1107 86-87 0.1443877275223997 1107 88-89 0.43882679212778086 1107 90-91 1.0097263884950642 1107 92-93 1.119564298760455 1107 94-95 0.6637543487192197 1107 96-97 0.7155915304362885 1107 98-99 1.4066208635534139 1107 100-101 2.0643435632160774 1108 1 0.1520142006711218 1108 2 0.11763311862133108 1108 3 0.07740331246541032 1108 4 0.1016844408245845 1108 5 -0.010411950739829479 1108 6 -0.0840747809875424 1108 7 -0.0049459130424054365 1108 8 -0.03413607614110248 1108 9 0.18750909525390824 1108 10-11 0.01803435689891586 1108 12-13 0.13310317640146252 1108 14-15 0.27280621711009445 1108 16-17 0.13830741389147505 1108 18-19 0.3179510033374271 1108 20-21 0.11863612806180157 1108 22-23 0.1457264401543128 1108 24-25 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0.6031011835006979 2316 62-63 0.11679574592817232 2316 64-65 0.08470397942418728 2316 66-67 -0.1977005478517917 2316 68-69 0.34167009242811375 2316 70-71 0.4099850556426503 2316 72-73 0.3804142506689523 2316 74-75 0.7013141941625918 2316 76-77 0.6991314801432722 2316 78-79 0.9847832528271283 2316 80-81 0.9476217943512033 2316 82-83 1.1352068487392408 2316 84-85 1.1565118794276188 2316 86-87 1.411739248481382 2316 88-89 1.943749475929895 2316 90-91 1.9181400442453977 2316 92-93 1.2993315046676734 2316 94-95 2.1172143190328043 2316 96-97 2.6806912053888468 2316 98-99 2.7107328076377364 2316 100-101 3.149156520020469 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 3.0 4 6.0 5 25.0 6 88.0 7 195.0 8 352.0 9 602.0 10 956.0 11 1528.0 12 2054.0 13 2791.0 14 3504.0 15 4770.0 16 6061.0 17 7496.0 18 6590.0 19 4700.0 20 4320.0 21 4438.0 22 4542.0 23 4827.0 24 5149.0 25 5736.0 26 6021.0 27 6829.0 28 7993.0 29 9551.0 30 11271.0 31 13312.0 32 15946.0 33 19224.0 34 24196.0 35 30601.0 36 36148.0 37 42674.0 38 23949.0 39 1146.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.128487215109075 22.60103380517904 16.00113893790911 20.269340041802774 2 15.832115028816684 23.89671699542627 38.821912576346215 21.44925539941083 3 19.159501370480232 27.374560382482883 32.687830886494204 20.778107360542684 4 12.30772600408015 21.32348339778971 36.43412308038899 29.934667517741147 5 11.267301241273033 40.19964356075911 34.49473641349458 14.03831878447328 6 26.933378390217648 39.10217900098875 17.655102066358776 16.309340542434825 7 23.38045532484762 37.28457177186198 20.991188875955892 18.343784027334507 8 24.397051277237512 35.35213206673426 20.44456125858897 19.80625539743927 9 24.489668206110213 17.96205209076459 22.054406187812113 35.49387351531308 10-11 23.152041952965618 28.753019437039264 27.41508028886469 20.679858321130425 12-13 25.303195284046108 27.63082141203269 27.51849209627154 19.54749120764966 14-15 21.796893578142402 29.182780760710397 28.06684063630333 20.953485024843864 16-17 18.525576039750185 31.800617028999113 29.272581634313322 20.401225296937383 18-19 17.93389153806681 29.60393747105721 33.35101190252694 19.11115908834904 20-21 19.664826843890413 27.977352657730385 33.390280228788846 18.96754026959036 22-23 18.875236235747632 29.209689733288275 32.99290354072016 18.922170490243932 24-25 18.979853969833364 29.356537955483798 33.51403275744192 18.149575317240917 26-27 18.62569000262858 29.26440400045061 34.12555857355647 17.984347423364333 28-29 17.553567629131777 29.951563849359818 33.88856556402458 18.606302957483823 30-31 18.613655990688244 29.284315197937396 33.95036233244471 18.15166647892965 32-33 17.799899560223974 29.884901023015104 33.93049630078115 18.384703115979782 34-35 18.4862694661278 29.883919057833936 33.776909921877326 17.852901554160937 36-37 18.497507736499067 29.39071745272835 33.57969141616263 18.532083394609952 38-39 18.072864105392444 29.390191430605427 33.788010357749144 18.74893410625298 40-41 18.561178543154945 29.25831009978691 33.670180642779144 18.510330714279007 42-43 18.206694348572984 29.33150386597535 33.29338544290529 19.16841634254638 44-45 18.638059000963743 29.32901110179356 32.78815224601674 19.244777651225952 46-47 19.440511521595266 28.97052680654139 32.02532576334774 19.563635908515607 48-49 18.920762462609044 28.994574400180227 32.094738357175935 19.989924780034794 50-51 18.45470531546077 29.48707117736142 31.98428641159464 20.073937095583176 52-53 18.950174595426727 29.81905280416526 31.374923340717658 19.85584925969036 54-55 18.760716654776655 29.239727656165908 31.044506189063693 20.95504949999374 56-57 19.50963090902264 29.68857557666554 30.194839735165647 20.606953779146174 58-59 19.236473547854168 29.840955456263536 30.396500581984753 20.526070413897546 60-61 19.088004856130866 29.998028761311158 30.4467202342958 20.46724614826218 62-63 18.556552647717744 30.142116922614804 30.419811261717918 20.881519167949534 64-65 18.337839021764978 30.419341919172954 30.032916557153406 21.209902501908658 66-67 18.64390869694457 30.20009699776279 29.383907758256544 21.772086547036093 68-69 18.573066808095884 29.632237100450727 29.75551832087223 22.03917777058116 70-71 18.87664426338252 29.192636954154622 29.86598705866156 22.064731723801298 72-73 18.829866456401206 28.46484311443197 30.275253757869308 22.43003667129751 74-75 18.564687918497103 29.262099650809148 29.370517778695604 22.80269465199815 76-77 18.266186059900626 28.6395949886732 30.230979111127798 22.863239840298377 78-79 18.236773927082943 28.51709658443785 30.072810673475264 23.17331881500394 80-81 18.274349920446316 28.238977081967676 30.008557692458123 23.47811530512789 82-83 18.145692463179504 28.245476249331176 30.204699131076907 23.404132156412413 84-85 18.070507836029307 28.062794623593668 29.970132814321232 23.896564726055793 86-87 17.819890828703695 28.35043078699394 29.70607607571588 24.123602308586484 88-89 18.04559506376801 27.96514975156135 29.334221955218464 24.655033229452183 90-91 18.21891104568026 27.455091038233242 29.71125594900983 24.614741967076668 92-93 18.00612652302609 27.66259691984205 29.76604692203233 24.565229635099534 94-95 17.72346963040839 27.398496852276 29.91792763363747 24.960105883678143 96-97 17.72691147573812 27.363765503948734 29.655565151003145 25.253757869310007 98-99 17.964555251004395 27.2753726579807 29.110345561271107 25.6497265297438 100-101 17.744544641013285 26.937458150027847 29.607564597583185 25.710432611375683 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2462.0 1 1437.5 2 410.0 3 411.5 4 357.0 5 366.5 6 601.0 7 1028.0 8 1339.0 9 1341.5 10 1040.0 11 716.5 12 629.0 13 601.0 14 599.5 15 617.5 16 667.5 17 761.0 18 873.0 19 987.5 20 1113.0 21 1252.5 22 1441.5 23 1721.0 24 2021.0 25 2396.0 26 3083.5 27 3889.5 28 4445.5 29 4950.5 30 5573.5 31 6250.5 32 6853.0 33 7493.0 34 8657.5 35 9867.5 36 10936.5 37 11523.0 38 11008.5 39 10637.0 40 10717.0 41 10745.0 42 10809.0 43 11043.0 44 11414.5 45 11601.5 46 11788.0 47 12789.5 48 14393.5 49 14082.5 50 12182.0 51 10471.0 52 8721.0 53 7163.0 54 5976.5 55 5126.0 56 4316.0 57 3502.5 58 2868.0 59 2224.0 60 1644.5 61 1222.5 62 860.5 63 594.0 64 444.0 65 347.0 66 251.5 67 221.0 68 198.0 69 145.0 70 127.0 71 104.0 72 68.0 73 57.5 74 55.5 75 51.5 76 42.0 77 30.5 78 24.0 79 18.5 80 11.5 81 6.5 82 3.5 83 2.0 84 1.5 85 0.5 86 0.0 87 0.0 88 0.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.05100188988598105 3 0.0 4 0.0 5 0.10231667480193743 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0014080276348890474 26-27 0.010012640959211004 28-29 0.0 30-31 0.0 32-33 4.693425449630158E-4 34-35 0.010794878534149364 36-37 0.002190265209827407 38-39 0.008917508354297301 40-41 0.004693425449630158 42-43 0.00406763538967947 44-45 0.002503160239802751 46-47 1.5644751498767193E-4 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 3.1289502997534387E-4 68-69 4.693425449630158E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 1.5644751498767193E-4 82-83 0.0015644751498767194 84-85 0.004536977934642486 86-87 0.002033817694839735 88-89 0.0 90-91 0.0015644751498767194 92-93 6.257900599506877E-4 94-95 0.0 96-97 0.0 98-99 0.0 100-101 6.257900599506877E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 319596.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.204574995201654 #Duplication Level Percentage of deduplicated Percentage of total 1 78.10243614857471 46.2402153826624 2 12.951223307158594 15.335433431365491 3 4.4517191049535 7.906864128203744 4 1.8423105050988153 4.362928418542826 5 0.9082316548140089 2.6885734560226044 6 0.5074913639130654 1.8027486308524954 7 0.2818021671731024 1.1678784278147205 8 0.1996157384145777 0.9454531964150737 9 0.11404901173060131 0.6077000941319717 >10 0.5470870592782703 5.910173174191542 >50 0.0517714944454562 2.0755168861308375 >100 0.03539479722291384 4.136959290664939 >500 0.0031696833333952697 1.4180822124347798 >1k 0.003697963888961148 5.40147327056653 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3762 1.1771111027672436 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3477 1.0879360192242706 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3449 1.079174958384961 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2449 0.7662799284096171 No Hit CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT 1778 0.5563273632961614 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1181 0.3695290304008811 No Hit ATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT 1174 0.3673387651910537 TruSeq Adapter, Index 11 (95% over 21bp) GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 883 0.27628631146822863 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 843 0.2637705102692149 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 824 0.25782550469968335 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 802 0.25094181404022575 No Hit ACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCT 668 0.2090138800235297 RNA PCR Primer, Index 11 (95% over 23bp) GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 514 0.16082804540732673 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 425 0.13298038773952114 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 401 0.12547090702011288 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 400 0.12515801199013754 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 382 0.11952590145058135 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 380 0.11890011139063068 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 359 0.11232931576114845 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 338 0.10575852013166623 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 328 0.10262956983191279 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 322 0.10075219965206073 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 4.6934254496301583E-4 0.0 22-23 0.0 0.0 0.0 0.00406763538967947 0.0 24-25 0.0 0.0 0.0 0.011733563624075394 0.0 26-27 0.0 0.0 0.0 0.02487515488303984 0.0 28-29 0.0 0.0 0.0 0.0541308401857345 0.0 30-31 0.0 0.0 0.0 0.1237499843552485 0.0 32-33 0.0 0.0 0.0 0.247499968710497 0.0 34-35 0.0 0.0 0.0 0.44008685966032113 0.0 36-37 0.0 0.0 0.0 0.7385887182567992 0.0 38-39 0.0 0.0 0.0 1.0699445550006883 0.0 40-41 0.0 0.0 0.0 1.3696979937170677 0.0 42-43 0.0 0.0 0.0 1.673362620308139 0.0 44-45 0.0 0.0 0.0 2.0446125733738847 0.0 46-47 0.0 0.0 0.0 2.465925731235685 0.0 48-49 0.0 0.0 0.0 2.8611121540945446 0.0 50-51 0.0 0.0 0.0 3.2425311956344887 0.0 52-53 0.0 0.0 0.0 3.6317726129238164 0.0 54-55 0.0 0.0 0.0 4.074206185308952 0.0 56-57 0.0 0.0 0.0 4.5922039074331344 0.0 58-59 0.0 0.0 0.0 5.071559093355361 0.0 60-61 0.0 0.0 0.0 5.600821036558655 0.0 62-63 0.0 0.0 0.0 6.102548217124118 0.0 64-65 0.0 0.0 0.0 6.628994105057635 0.0 66-67 0.0 0.0 0.0 7.24758757931889 0.0 68-69 0.0 0.0 0.0 7.9559819271830685 0.0 70-71 0.0 0.0 0.0 8.684870899510631 0.0 72-73 0.0 0.0 0.0 9.372301280366463 0.0 74-75 0.0 0.0 0.0 10.055038235772663 0.0 76-77 0.0 0.0 0.0 10.823664876907095 0.0 78-79 0.0 0.0 0.0 11.65799947433635 0.0 80-81 0.0 0.0 0.0 12.516583436588693 0.0 82-83 0.0 0.0 0.0 13.358740409767332 0.0 84-85 0.0 0.0 0.0 14.210753576390193 0.0 86-87 0.0 0.0 0.0 15.1154895555639 1.5644751498767193E-4 88-89 0.0 0.0 0.0 16.063405048874202 3.1289502997534387E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGTATAG 140 4.456524E-10 33.978104 5 CAGGCCG 65 0.0058408976 29.227638 9 TAGGACG 190 3.8562575E-10 27.54015 4 GTATAGC 145 7.8650373E-7 26.20409 6 GGTATCA 3495 0.0 25.740332 1 TAGGACC 130 9.879057E-6 25.614264 4 AGGACGT 205 9.349606E-10 25.525015 5 CACCGCA 50 0.0016538753 23.747458 86-87 GACGTGA 105 0.0021941424 22.616627 7 GTAGGAC 170 3.0977062E-6 22.385574 3 GAGTACT 2985 0.0 22.355227 12-13 GGACGTG 215 4.4014087E-8 22.090658 6 GGCCATA 110 0.0028382938 21.635994 1 ATGGGCA 220 5.5213604E-8 21.62243 5 GTACTTT 2990 0.0 21.603039 14-15 TCACCGC 55 0.0028751583 21.591976 84-85 GTATCAA 4440 0.0 21.33387 1 TACATGG 1890 0.0 21.155193 2 ATATAGG 135 3.734672E-4 21.155193 1 TGGAGTG 135 3.7505274E-4 21.14193 5 >>END_MODULE