FastQCFastQC Report
Thu 26 May 2016
SRR938296_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938296_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences320189
Sequences flagged as poor quality0
Sequence length101
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39171.2233399648332703No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35181.0987260649179078No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA33291.039698428115894No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23700.7401878265649351No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT12780.39913925837552194No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10210.3188741649463286No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9140.2854564023123843No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT8640.2698406253806346No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7530.23517360059215026No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT5250.16396565778337172No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4900.15303461393114692No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC3900.12180306006764755No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC3890.12149074452901257No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA3620.11305822498586772No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG3570.11149664729269275No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3550.11087201621542277No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC3530.11024738513815278No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3500.1093104385222478No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT3470.10837349190634282No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT3330.1040010743654529No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG3230.10087791897910298No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCTATC500.00161420437.999387
CATTCCG1201.5002843E-731.6661439
CTATACC953.4723183E-530.0135734
GCGCACT404.4959853E-429.68237592-93
CCTATAC700.00836420227.155143
CGCACTA350.00839590827.1381792-93
GTTCTAA1257.1492996E-626.703861
GGTATCA34750.026.0726261
TACATGG18000.025.0807862
GTACATG18700.024.4802361
GTACCCA1000.001614005923.8427311
GTAAGCG701.7620448E-523.7774794-95
CCTTAGA1000.001646705223.7607462
CATGGGG7350.023.2758334
ACATGGG19400.023.0258773
GACGAGG1253.765308E-1022.8263794-95
GACGTGA1252.2512188E-422.7996257
TAGGCAT1903.2573735E-722.510185
ATGGGGT2606.166374E-1021.9329975
CATGGGT3101.0913936E-1121.4613194
Produced by FastQC (version 0.11.5)