##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938296_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 320189 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.219489114241902 31.0 31.0 34.0 30.0 34.0 2 31.37698671722014 33.0 31.0 34.0 30.0 34.0 3 31.39282736133971 33.0 31.0 34.0 28.0 34.0 4 35.04365234283501 37.0 35.0 37.0 33.0 37.0 5 35.06727901333275 37.0 35.0 37.0 33.0 37.0 6 35.089796963668334 37.0 35.0 37.0 33.0 37.0 7 35.04657249312125 37.0 35.0 37.0 32.0 37.0 8 35.032786885245905 37.0 35.0 37.0 32.0 37.0 9 36.54631170964649 39.0 37.0 39.0 32.0 39.0 10-11 36.432135395032304 39.0 37.0 39.0 32.0 39.0 12-13 36.30784630327713 39.0 37.0 39.0 32.0 39.0 14-15 37.421279306909355 40.0 37.0 41.0 32.0 41.0 16-17 37.35973128371056 40.0 37.0 41.0 32.0 41.0 18-19 37.42678855301088 40.0 37.0 41.0 32.0 41.0 20-21 37.43606432450834 40.0 37.0 41.0 32.0 41.0 22-23 37.28567502318943 40.0 37.0 41.0 32.0 41.0 24-25 37.16354090865083 40.0 37.0 41.0 32.0 41.0 26-27 36.93464172722985 39.5 36.0 41.0 31.5 41.0 28-29 36.71090668324021 39.0 36.0 41.0 31.0 41.0 30-31 36.368921168434895 39.0 35.5 40.5 30.5 41.0 32-33 35.95098051463354 38.0 35.0 40.0 30.0 41.0 34-35 35.60968365559091 38.0 35.0 40.0 29.5 41.0 36-37 35.3052212911749 38.0 35.0 40.0 28.0 41.0 38-39 35.014307174824864 38.0 35.0 40.0 26.5 41.0 40-41 34.69955401341083 38.0 35.0 40.0 24.5 41.0 42-43 34.18676313052603 38.0 34.0 40.0 22.5 41.0 44-45 33.568279984634074 38.0 33.0 40.0 17.0 41.0 46-47 32.85897391852937 38.0 33.0 40.0 5.5 41.0 48-49 32.237391040916464 38.0 32.5 40.0 2.0 41.0 50-51 31.44452651402765 36.5 30.5 39.5 2.0 40.5 52-53 31.44246366989497 37.0 31.0 39.0 2.0 40.0 54-55 31.516910324839394 37.0 31.0 40.0 2.0 41.0 56-57 31.178614818123044 37.0 31.0 40.0 2.0 41.0 58-59 30.833318758608197 37.0 30.0 40.0 2.0 41.0 60-61 30.401127146778933 36.0 29.0 39.0 2.0 41.0 62-63 29.837984752755403 35.5 28.0 39.0 2.0 40.0 64-65 29.32103851162907 35.0 27.5 39.0 2.0 40.0 66-67 28.752108910674632 35.0 26.0 38.0 2.0 40.0 68-69 28.055362613956135 34.0 25.5 38.0 2.0 40.0 70-71 27.38122483907942 34.0 22.5 37.0 2.0 39.0 72-73 26.62315694792763 33.5 21.0 36.5 2.0 39.0 74-75 25.857442010812363 32.5 17.5 36.0 2.0 38.5 76-77 25.067494511054406 32.0 9.5 35.0 2.0 37.5 78-79 24.232233149795903 31.0 4.5 35.0 2.0 37.0 80-81 23.3708871947506 30.5 2.0 34.5 2.0 36.0 82-83 22.52630789939692 29.5 2.0 34.0 2.0 36.0 84-85 21.685940179081733 29.0 2.0 34.0 2.0 35.0 86-87 20.82630102845507 28.5 2.0 34.0 2.0 35.0 88-89 19.847944495282473 26.5 2.0 33.5 2.0 35.0 90-91 18.807777593858628 25.5 2.0 33.0 2.0 35.0 92-93 17.721375500095256 22.5 2.0 32.0 2.0 34.0 94-95 16.47986189406882 12.5 2.0 32.0 2.0 34.0 96-97 15.045242028926666 2.0 2.0 31.0 2.0 34.0 98-99 13.31733132618547 2.0 2.0 31.0 2.0 34.0 100-101 9.46522678792838 2.0 2.0 22.5 2.0 30.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.6233332553513087 1101 2 0.5582560023611087 1101 3 0.30484777970066546 1101 4 0.4956856266074965 1101 5 0.24045416499022565 1101 6 0.5211823448088992 1101 7 0.39224183153961434 1101 8 0.5664003649280573 1101 9 0.4111031010160815 1101 10-11 0.6039487522181588 1101 12-13 0.3770068333513308 1101 14-15 0.7041551742167087 1101 16-17 0.8019212838403362 1101 18-19 0.2799854672683253 1101 20-21 0.6152960101876133 1101 22-23 0.957722058511024 1101 24-25 0.7243326010499231 1101 26-27 0.6939331433404021 1101 28-29 0.6938069693583984 1101 30-31 0.7796169201915504 1101 32-33 0.7070458748422368 1101 34-35 0.6266987791789091 1101 36-37 0.7609491233854797 1101 38-39 0.8577518063513665 1101 40-41 0.8449597868337548 1101 42-43 1.1223893694427147 1101 44-45 0.7018276448661354 1101 46-47 0.5086126034599943 1101 48-49 0.5521693583642815 1101 50-51 0.8688945626558429 1101 52-53 0.7941262078208489 1101 54-55 0.980692360177148 1101 56-57 1.1553594413882813 1101 58-59 0.9812814415595774 1101 60-61 1.0199773072149796 1101 62-63 1.0795535020274762 1101 64-65 0.6143297152551845 1101 66-67 0.956669766568023 1101 68-69 1.0243379298355677 1101 70-71 0.9024165747652688 1101 72-73 0.9482257743334763 1101 74-75 0.7499900178604619 1101 76-77 1.1387307670380196 1101 78-79 0.8362560533501799 1101 80-81 1.2981597576023667 1101 82-83 1.2616531104103608 1101 84-85 1.3782638148190145 1101 86-87 1.4921449757733107 1101 88-89 2.1843017846219794 1101 90-91 2.257256436960237 1101 92-93 2.776068504305769 1101 94-95 2.876563457136477 1101 96-97 3.0469611894262147 1101 98-99 3.0728058909836005 1101 100-101 2.854635557134719 1102 1 0.21965759366749182 1102 2 0.2158305833486196 1102 3 0.15118829610524642 1102 4 0.07858215155410875 1102 5 0.17208556342419 1102 6 0.19830005658007366 1102 7 -0.16415498461259403 1102 8 0.13868406388618837 1102 9 0.19237534588762628 1102 10-11 0.134460626935045 1102 12-13 0.37846286130707085 1102 14-15 0.5640043944328426 1102 16-17 0.7121490713249656 1102 18-19 0.6799239905324157 1102 20-21 0.38438615449616265 1102 22-23 0.5238580838782667 1102 24-25 0.5832111359622445 1102 26-27 0.5882255137539119 1102 28-29 0.3760693132398387 1102 30-31 0.5182275459600092 1102 32-33 0.34125929618436857 1102 34-35 0.17358287935363137 1102 36-37 0.7180933672216341 1102 38-39 0.7872153409401221 1102 40-41 1.0468623300292492 1102 42-43 1.2054800314500866 1102 44-45 1.160185245332066 1102 46-47 1.503451793261327 1102 48-49 1.348082773235177 1102 50-51 1.0842087411325103 1102 52-53 1.2946762628263535 1102 54-55 1.3398621006806088 1102 56-57 1.875721183444842 1102 58-59 2.031595296474478 1102 60-61 2.1936167299807927 1102 62-63 2.072143937541618 1102 64-65 1.91364052868947 1102 66-67 2.1145517312350783 1102 68-69 2.3648866905939983 1102 70-71 2.0943857716849585 1102 72-73 2.1519240853410473 1102 74-75 2.0383320873615247 1102 76-77 1.8719379112818544 1102 78-79 2.384580003392241 1102 80-81 2.5073262624881494 1102 82-83 2.368379959565864 1102 84-85 2.259387221277308 1102 86-87 2.333470284774794 1102 88-89 2.814810423721184 1102 90-91 3.0644298013555016 1102 92-93 3.308715886691065 1102 94-95 3.048035016057078 1102 96-97 3.6769197735199146 1102 98-99 3.4743460449990042 1102 100-101 2.990094691283426 1103 1 0.5079029430893129 1103 2 0.37855557077948987 1103 3 0.36388814527568414 1103 4 0.45266963270041316 1103 5 0.43506193345938016 1103 6 0.5759068941869074 1103 7 0.7484845327328102 1103 8 0.5270299663497369 1103 9 0.4342054112471061 1103 10-11 0.6664163835966761 1103 12-13 0.4429575822723777 1103 14-15 0.6985192260064963 1103 16-17 0.7728479149649843 1103 18-19 0.8844840709748425 1103 20-21 0.9163368835057213 1103 22-23 0.9559043382774632 1103 24-25 1.1130200851829528 1103 26-27 1.0445754383391304 1103 28-29 0.7584121991121364 1103 30-31 0.8409776716513164 1103 32-33 1.0727122876373585 1103 34-35 1.0919539200776143 1103 36-37 1.4799184049290162 1103 38-39 1.533288744660581 1103 40-41 1.8477371414923027 1103 42-43 1.3396881762464758 1103 44-45 1.081995200083199 1103 46-47 1.4334920144780199 1103 48-49 1.541491521349812 1103 50-51 1.0591458954814321 1103 52-53 1.1230523312024197 1103 54-55 1.1647238402482323 1103 56-57 1.3066161055162375 1103 58-59 0.9671407967258645 1103 60-61 1.0691471472759098 1103 62-63 1.3863420270338231 1103 64-65 0.9517249932445218 1103 66-67 1.1211429831204498 1103 68-69 0.9392858861696638 1103 70-71 0.5468644041635926 1103 72-73 0.8690432406955004 1103 74-75 0.9285734146616775 1103 76-77 0.8089364029862303 1103 78-79 0.9114528037944503 1103 80-81 0.9023740251829722 1103 82-83 1.3380481345281545 1103 84-85 1.2928512735648887 1103 86-87 1.5868136734123048 1103 88-89 1.6090034855613062 1103 90-91 1.957144733482199 1103 92-93 1.8466143281189211 1103 94-95 1.461021441396447 1103 96-97 1.9924803567275564 1103 98-99 2.430414421067379 1103 100-101 1.1986467274825205 1104 1 0.40560230794464047 1104 2 0.38315684106883197 1104 3 0.509256293946585 1104 4 0.3058712323240371 1104 5 0.2943797410340707 1104 6 0.5076782421050439 1104 7 0.32783997851027635 1104 8 0.33689117547699965 1104 9 0.4770188669046007 1104 10-11 0.4653954473463884 1104 12-13 0.3114544065490179 1104 14-15 0.49593159956565813 1104 16-17 0.6726785430524913 1104 18-19 0.5156108646704496 1104 20-21 0.5349677938797512 1104 22-23 0.5216394594254226 1104 24-25 0.6204630397818747 1104 26-27 0.6380546197082211 1104 28-29 0.6530675743179657 1104 30-31 0.6408365283909063 1104 32-33 0.8004845765105699 1104 34-35 0.43639993278050326 1104 36-37 0.47834700996312307 1104 38-39 0.7089038940372063 1104 40-41 0.4130253089822986 1104 42-43 0.403447291748698 1104 44-45 0.2310613021768262 1104 46-47 0.3063689846026918 1104 48-49 0.21240189537945042 1104 50-51 0.015956057796181256 1104 52-53 0.2905987908589722 1104 54-55 0.37288662547523543 1104 56-57 0.5588626357444184 1104 58-59 0.5689521352344471 1104 60-61 0.755663261131474 1104 62-63 0.2870806767632992 1104 64-65 0.17155562133203262 1104 66-67 0.4994680830969216 1104 68-69 0.6441035761249658 1104 70-71 0.09795355965641761 1104 72-73 0.6490008977173751 1104 74-75 0.9269361528409306 1104 76-77 0.794361008961296 1104 78-79 1.243892229811859 1104 80-81 1.2753450908145858 1104 82-83 1.291522611121902 1104 84-85 1.0372549432896605 1104 86-87 1.0366842920902606 1104 88-89 1.1964887831383457 1104 90-91 1.3855756909039805 1104 92-93 1.6810250550067103 1104 94-95 1.842197635233191 1104 96-97 1.7507489993998693 1104 98-99 2.401461233582081 1104 100-101 1.7739137234834619 1105 1 -0.1468223316359598 1105 2 -0.02092245119853331 1105 3 -0.07377668988915076 1105 4 -0.2214878764570969 1105 5 -0.173619385221933 1105 6 -0.0020967688167559118 1105 7 0.008814203062478043 1105 8 -0.10828138896162187 1105 9 -0.2681755411338429 1105 10-11 -0.13503050285020635 1105 12-13 -0.19471641040161103 1105 14-15 0.16026373150100426 1105 16-17 0.1600365596536264 1105 18-19 0.040976744967522905 1105 20-21 0.09522882489766005 1105 22-23 0.08752521739833696 1105 24-25 0.11268125734412848 1105 26-27 -0.3977688840051954 1105 28-29 -0.19757681187687837 1105 30-31 -0.03983734666370964 1105 32-33 -0.295149433974359 1105 34-35 0.02346033033402506 1105 36-37 0.03549532800594335 1105 38-39 0.5410085248803682 1105 40-41 0.5669953832505428 1105 42-43 0.2271011799094751 1105 44-45 0.27721955905756346 1105 46-47 0.3155432965292988 1105 48-49 0.4377186275769134 1105 50-51 0.5705241006096422 1105 52-53 0.8546961040961953 1105 54-55 0.8660058915302891 1105 56-57 1.1932690359191689 1105 58-59 1.2799613095463798 1105 60-61 1.4082817626605255 1105 62-63 1.218123162564961 1105 64-65 1.475877923647456 1105 66-67 1.392475400702864 1105 68-69 1.3094186700524446 1105 70-71 0.8746437081928953 1105 72-73 0.7284296875819471 1105 74-75 1.0388572974455599 1105 76-77 0.9749666227664484 1105 78-79 1.1998227671644166 1105 80-81 1.2819527797617276 1105 82-83 1.4617798195098395 1105 84-85 1.800470321183937 1105 86-87 1.276076493925121 1105 88-89 1.900944877502699 1105 90-91 1.5889396052770728 1105 92-93 1.5346729361775289 1105 94-95 1.8160672289422308 1105 96-97 1.7595820050792046 1105 98-99 1.9123420767450359 1105 100-101 0.8530148593506546 1106 1 0.4883359232827722 1106 2 0.3673902091949657 1106 3 0.4100235667404455 1106 4 0.4930473388266279 1106 5 0.4904096994658289 1106 6 0.30974383568852204 1106 7 0.6527406359110657 1106 8 0.23275083231012417 1106 9 0.5479778663369288 1106 10-11 0.5704513905059443 1106 12-13 0.7954817646768007 1106 14-15 0.672747692151134 1106 16-17 0.33527536775171995 1106 18-19 0.44048130727149015 1106 20-21 0.5879398314559765 1106 22-23 0.4790457477661789 1106 24-25 0.6517994495311825 1106 26-27 0.5559665023649316 1106 28-29 0.9701947877809474 1106 30-31 0.596942664742059 1106 32-33 0.8000084645143772 1106 34-35 0.9822302904138667 1106 36-37 0.6571270645328866 1106 38-39 1.1730123743243297 1106 40-41 1.5118734841915114 1106 42-43 1.2448424830175426 1106 44-45 1.8375364253250268 1106 46-47 2.132409563994372 1106 48-49 1.7607867769923757 1106 50-51 1.4242541405297935 1106 52-53 1.2564194431324047 1106 54-55 1.2457464104923623 1106 56-57 1.7450315822550628 1106 58-59 1.2150910500653396 1106 60-61 1.8519569123611213 1106 62-63 1.7169914973240132 1106 64-65 1.768284182558201 1106 66-67 1.9323866496335782 1106 68-69 1.974882385478736 1106 70-71 1.937366476941115 1106 72-73 1.6256530979041592 1106 74-75 1.9273481728376325 1106 76-77 2.056099000867391 1106 78-79 2.362604346862163 1106 80-81 2.4684725972695674 1106 82-83 2.8329214647907115 1106 84-85 2.7753063633852513 1106 86-87 2.654132881150673 1106 88-89 2.7451849687487773 1106 90-91 2.559498487512599 1106 92-93 2.365582545530252 1106 94-95 2.4479997923867707 1106 96-97 3.065211816884167 1106 98-99 3.0034544947661335 1106 100-101 2.1739622502830738 1107 1 0.22807678055128733 1107 2 -0.010379674765925273 1107 3 -0.14243084646703608 1107 4 -0.23530950680414975 1107 5 -0.05517709305082974 1107 6 0.10978781552545769 1107 7 0.2640603693255912 1107 8 0.018433703855166073 1107 9 -0.060144871238769326 1107 10-11 -0.05643889671622304 1107 12-13 0.16535219331105822 1107 14-15 0.28589428033070163 1107 16-17 0.32170927958906503 1107 18-19 0.22060749557284964 1107 20-21 0.3068410121454406 1107 22-23 0.3180034256307849 1107 24-25 0.29039513950716156 1107 26-27 0.31853458653557 1107 28-29 0.36789937859931143 1107 30-31 0.2195956638286347 1107 32-33 0.15811428676816774 1107 34-35 0.39192784445832274 1107 36-37 0.36565069523758353 1107 38-39 0.3311114304493685 1107 40-41 0.18760676339582005 1107 42-43 0.5835126004098754 1107 44-45 0.40769272289936254 1107 46-47 -0.21129286166279115 1107 48-49 -0.13330639260064459 1107 50-51 -0.04194058865589767 1107 52-53 -0.25428795562939044 1107 54-55 -0.3571579502776281 1107 56-57 -0.21520049596138335 1107 58-59 0.20933984061981903 1107 60-61 0.3682040790447054 1107 62-63 0.5285801843567306 1107 64-65 0.5112643239702948 1107 66-67 0.25413298746638446 1107 68-69 0.3125714142009528 1107 70-71 0.5341221182009654 1107 72-73 0.7085043445633836 1107 74-75 0.9730279996247369 1107 76-77 1.0515656945985725 1107 78-79 1.3782933079230908 1107 80-81 1.4806008830708457 1107 82-83 1.596944266644627 1107 84-85 1.4774275447513325 1107 86-87 1.3383999402528062 1107 88-89 1.0258439896899425 1107 90-91 1.3954367804183114 1107 92-93 1.538304897436607 1107 94-95 1.5220296986758868 1107 96-97 1.0188384618992234 1107 98-99 1.0200145949048416 1107 100-101 0.7025406866307176 1108 1 0.42500752390175833 1108 2 0.380863263087182 1108 3 0.2102321383612491 1108 4 0.35562555318442435 1108 5 0.014984146078226956 1108 6 0.17894924509442944 1108 7 -0.004428172539448383 1108 8 0.040461254163048466 1108 9 0.2038683325954196 1108 10-11 0.2770213965612456 1108 12-13 0.12477262116607335 1108 14-15 0.10072320180597671 1108 16-17 0.06088594435132677 1108 18-19 0.08775978402584172 1108 20-21 0.11305252741237126 1108 22-23 0.21061911787987952 1108 24-25 0.1473588924431084 1108 26-27 0.2258935022976445 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-0.6415698660454758 2316 56-57 -0.6133019075052282 2316 58-59 -0.5920345107253056 2316 60-61 -0.4354682373394745 2316 62-63 -0.08113325630352719 2316 64-65 -0.6349641796515471 2316 66-67 -0.8855558353025081 2316 68-69 -0.5777240004953939 2316 70-71 -0.31714331225791526 2316 72-73 -0.5169707453184884 2316 74-75 -0.48958911246636916 2316 76-77 -0.10235368623489549 2316 78-79 -0.278588189694819 2316 80-81 -0.5282987503173899 2316 82-83 -0.7195019016425697 2316 84-85 -0.8600013910119131 2316 86-87 -0.35150318214592957 2316 88-89 -0.5722600784608822 2316 90-91 -0.27260689420046447 2316 92-93 -0.22409702948899124 2316 94-95 -0.09833230830213324 2316 96-97 -0.09642728735249939 2316 98-99 -0.5886981904590254 2316 100-101 -0.0014690188401207394 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3902.0 3 708.0 4 519.0 5 454.0 6 486.0 7 539.0 8 648.0 9 784.0 10 1018.0 11 1516.0 12 2209.0 13 3031.0 14 3714.0 15 4771.0 16 6249.0 17 7562.0 18 6377.0 19 4595.0 20 4407.0 21 4658.0 22 4948.0 23 5370.0 24 6099.0 25 6920.0 26 7844.0 27 8720.0 28 9961.0 29 11866.0 30 13760.0 31 15770.0 32 18599.0 33 21954.0 34 26472.0 35 30512.0 36 33784.0 37 30257.0 38 9064.0 39 142.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.996996921687995 21.409315185294318 15.745785345730173 19.847902547287514 2 15.904255817658692 23.331865055935122 39.16989219095104 21.59398693545515 3 19.526817625153083 26.944465272050188 31.933405813401315 21.595311289395415 4 12.405716678227504 20.106172237740825 37.58834667516545 29.899764408866226 5 11.717221593714648 39.50097192967104 34.58663299810614 14.195173478508167 6 27.414163760670938 38.35726873898534 18.28964965565513 15.938917844688591 7 24.05512561353958 36.83580727905545 21.692197619888212 17.416869487516752 8 24.497912917416517 34.471543191361725 20.770845830833835 20.259698060387922 9 25.128094577537833 18.04790112348317 22.65461952786213 34.16938477111686 10-11 23.31847741697636 28.33106042499481 27.744634452291578 20.60582770573726 12-13 25.59441804669122 27.853690843259827 27.572218942028037 18.97967216802092 14-15 22.129771218911028 28.895112453707245 28.789679781449784 20.18543654593194 16-17 18.47183500066381 31.363150044123735 29.841701223750285 20.323313731462175 18-19 17.874363688334977 29.41755322056878 34.07756481046152 18.630518280634725 20-21 20.029741960376334 27.811232276275323 33.91614454217844 18.242881221169903 22-23 19.069183293786484 28.691642363188468 33.64517807016585 18.593996272859197 24-25 18.990211118644492 28.775030697656977 34.13985633808978 18.094901845608753 26-27 18.662188549558696 28.752479887526363 34.5822072951652 18.003124267749744 28-29 17.9461024902449 29.80920931365964 34.033346975372474 18.211341220722986 30-31 18.743380581790234 29.129032963531102 33.897044810532336 18.230541644146324 32-33 18.146828514704254 29.40162983119048 34.058848246377245 18.392693407728032 34-35 18.901947552906336 29.37309040469362 33.579042406293155 18.145919636106893 36-37 18.620660568343705 28.8714353552773 33.79226962585289 18.715634450526107 38-39 18.267547318919917 29.41808544034435 33.78131232595587 18.53305491477987 40-41 18.673190661296292 29.20695358291309 33.5522401113223 18.567615644468322 42-43 18.214686194951756 29.259942964413664 33.15435528859819 19.371015552036383 44-45 18.819027986006997 29.30097451274363 32.63524487756122 19.244752623688154 46-47 19.240302691896197 29.27083740072315 32.157812121732746 19.331047785647907 48-49 19.04598286380064 29.010029955550838 32.20705876473179 19.736928415916736 50-51 18.61157355205662 29.68865664462567 31.78853764199887 19.911232161318836 52-53 19.086750717209437 29.76485525409594 31.252996506483388 19.89539752221124 54-55 18.82398505775129 29.099748255592413 31.37606741502845 20.70019927162784 56-57 19.42395147486913 29.28749016129234 30.86558763633638 20.422970727502154 58-59 19.579105834144087 28.92788486314537 30.887122843565486 20.60588645914506 60-61 19.14132044002174 28.598445806238015 31.678098236539793 20.582135517200452 62-63 19.12770649308462 28.416303302140207 31.665032046877144 20.790958157898025 64-65 18.814642685918727 28.902142119819413 31.19574230846713 21.087472885794732 66-67 19.18652135279944 28.759090575472275 30.57587829364999 21.478509778078305 68-69 19.284039959834026 28.127269291614027 30.949975247640715 21.63871550091123 70-71 19.490707480868345 27.857098235202248 30.92347337185694 21.728720912072465 72-73 19.471475579835424 27.54630171900842 31.301976695699434 21.680246005456723 74-75 19.03841949086366 28.294549429954706 30.649226924878963 22.01780415430267 76-77 19.16913149535717 27.467665716132945 30.945963250887807 22.417239537622084 78-79 19.323635284655687 27.623821905721226 30.556505579110933 22.496037230512155 80-81 19.562121084342994 27.297544642159938 30.377595991966444 22.762738281530623 82-83 19.521885595503406 27.31939522760176 30.61562656663132 22.543092610263514 84-85 19.641189496797004 26.998085386155434 30.416592487092753 22.944132629954805 86-87 19.5672135448387 27.255833419222252 30.081578856959034 23.095374178980013 88-89 20.145999537699367 26.772360669461293 29.83713273650738 23.244507056331955 90-91 20.36709600227424 26.490985826614228 29.765203510076315 23.376714661035216 92-93 20.533207145244365 26.371280340582903 29.901111017407082 23.19440149676565 94-95 20.444169552253726 26.142872092351283 29.72740378382853 23.685554571566463 96-97 20.58323727770541 26.09370656020892 29.394584024465292 23.928472137620382 98-99 21.083011742804622 25.755614103859553 28.846162856254892 24.315211297080932 100-101 21.904073903811213 24.85653616539543 29.28100773375614 23.958382197037217 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2135.0 1 1348.5 2 553.5 3 501.0 4 379.5 5 366.0 6 566.5 7 948.0 8 1301.0 9 1386.0 10 1083.5 11 703.5 12 619.5 13 632.0 14 641.5 15 681.5 16 708.0 17 762.5 18 882.5 19 1011.5 20 1179.5 21 1324.0 22 1488.0 23 1743.0 24 2051.5 25 2466.0 26 3073.0 27 3810.0 28 4328.5 29 4853.0 30 5681.0 31 6336.5 32 6780.5 33 7471.0 34 8626.5 35 9829.5 36 10671.0 37 11201.5 38 11048.5 39 10652.0 40 10552.0 41 10467.5 42 10430.0 43 10590.5 44 10736.0 45 10989.5 46 11476.0 47 12352.0 48 13469.5 49 13154.0 50 11645.0 51 10238.5 52 9008.5 53 7853.0 54 6792.0 55 5848.5 56 4895.5 57 4000.5 58 3242.0 59 2556.0 60 1999.5 61 1541.0 62 1115.5 63 841.5 64 654.0 65 499.5 66 414.0 67 375.0 68 312.0 69 255.5 70 214.0 71 183.0 72 158.5 73 129.0 74 109.5 75 99.0 76 79.0 77 50.5 78 32.5 79 26.5 80 19.0 81 9.5 82 5.0 83 4.5 84 2.5 85 2.5 86 2.0 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.36946928220519754 2 0.02685913632260946 3 0.03154386940213436 4 0.0446611220248041 5 0.0646493164974437 6 0.05028280172023399 7 0.037477864636199246 8 0.039039442329374216 9 0.03529165586575429 10-11 0.03576012917370678 12-13 0.02685913632260946 14-15 0.024829085321482 16-17 0.02076898331922708 18-19 0.02576603193738698 20-21 0.03201234271008686 22-23 0.03263697378735684 24-25 0.04122565109981917 26-27 0.03560397140438928 28-29 0.03123155386349937 30-31 0.03373007817257932 32-33 0.029513818401006902 34-35 0.029669976170324405 36-37 0.03154386940213436 38-39 0.014834988085162202 40-41 0.011711832698812263 42-43 0.01186799046812976 44-45 0.009057150620414818 46-47 0.019207405626052113 48-49 0.0149911458544797 50-51 0.008744835081779824 52-53 0.0076517306965573455 54-55 0.007183257388604856 56-57 0.007807888465874843 58-59 0.004216259771572415 60-61 0.007183257388604856 62-63 0.010306412774954792 64-65 0.004840890848842402 66-67 0.0026546820783974463 68-69 0.00546552192611239 70-71 0.012180306006764756 72-73 0.01046257054427229 74-75 0.012180306006764756 76-77 0.0056216796954298865 78-79 0.006714784080652365 80-81 0.0099940972363198 82-83 0.011087201621542277 84-85 0.006246310772699873 86-87 0.00249852430907995 88-89 0.015928092470384677 90-91 0.025609874168069483 92-93 0.0099940972363198 94-95 0.0198320367033221 96-97 0.01936356339536961 98-99 0.011087201621542277 100-101 0.13179715730396735 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 320189.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.71045390627462 #Duplication Level Percentage of deduplicated Percentage of total 1 79.43194669069197 49.01781484941609 2 12.302152090021405 15.183427789984915 3 4.14505971642457 7.673805497075224 4 1.7409044328455816 4.2972801103338565 5 0.7901925770644167 2.4381571302007017 6 0.4611320529516391 1.7074000979026747 7 0.27704762033198255 1.1967714083037917 8 0.1599320849579661 0.7895585245546369 9 0.11090790514134195 0.6159759451259634 >10 0.4987607386481278 5.627716351853112 >50 0.046041557245761415 1.9223679015744388 >100 0.030863021890015472 3.757000643112714 >500 0.0020238047140993753 0.9541588934889431 >1k 0.003035707071149063 4.818564857072926 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3917 1.2233399648332703 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3518 1.0987260649179078 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3329 1.039698428115894 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2370 0.7401878265649351 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1278 0.39913925837552194 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1021 0.3188741649463286 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 914 0.2854564023123843 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 864 0.2698406253806346 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 753 0.23517360059215026 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 525 0.16396565778337172 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 490 0.15303461393114692 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 390 0.12180306006764755 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 389 0.12149074452901257 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 362 0.11305822498586772 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 357 0.11149664729269275 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 355 0.11087201621542277 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 353 0.11024738513815278 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 350 0.1093104385222478 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 347 0.10837349190634282 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 333 0.1040010743654529 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 323 0.10087791897910298 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 3.123155386349937E-4 0.0 14-15 0.0 0.0 0.0 3.123155386349937E-4 0.0 16-17 0.0 0.0 0.0 3.123155386349937E-4 0.0 18-19 0.0 0.0 0.0 6.246310772699874E-4 0.0 20-21 0.0 0.0 0.0 0.0014054199238574716 0.0 22-23 0.0 0.0 0.0 0.006090153003382377 0.0 24-25 0.0 0.0 0.0 0.014054199238574715 0.0 26-27 0.0 0.0 0.0 0.02685913632260946 0.0 28-29 0.0 0.0 0.0 0.05387443041453642 0.0 30-31 0.0 0.0 0.0 0.12742473976307744 0.0 32-33 0.0 0.0 0.0 0.24563617113642255 0.0 34-35 0.0 0.0 0.0 0.43318165208673626 0.0 36-37 0.0 0.0 0.0 0.7236351030172804 0.0 38-39 0.0 0.0 0.0 1.0699930353634883 0.0 40-41 0.0 0.0 0.0 1.3788731030734973 0.0 42-43 0.0 0.0 0.0 1.6708881316972164 0.0 44-45 0.0 0.0 0.0 2.0364534696694765 0.0 46-47 0.0 0.0 0.0 2.4512085049767482 0.0 48-49 0.0 0.0 0.0 2.849723132275 0.0 50-51 0.0 0.0 0.0 3.22778109179266 0.0 52-53 0.0 0.0 0.0 3.5999050560762553 0.0 54-55 0.0 0.0 0.0 4.033711339240261 0.0 56-57 0.0 0.0 0.0 4.534353147672157 0.0 58-59 0.0 0.0 0.0 5.044832895571053 0.0 60-61 0.0 0.0 0.0 5.5781116777903055 0.0 62-63 0.0 0.0 0.0 6.074537226450627 0.0 64-65 0.0 0.0 0.0 6.602038171205132 0.0 66-67 0.0 0.0 0.0 7.243846603100044 0.0 68-69 0.0 0.0 0.0 7.934532416791333 0.0 70-71 0.0 0.0 0.0 8.653170471190453 0.0 72-73 0.0 0.0 0.0 9.358691272966905 0.0 74-75 0.0 0.0 0.0 10.058590395047926 0.0 76-77 0.0 0.0 0.0 10.817829469469594 0.0 78-79 0.0 0.0 0.0 11.651243484317074 0.0 80-81 0.0 0.0 0.0 12.510267373332624 0.0 82-83 0.0 0.0 0.0 13.347741490182361 0.0 84-85 0.0 0.0 0.0 14.17881313849008 0.0 86-87 0.0 0.0 0.0 15.02721829919204 0.0 88-89 0.0 0.0 0.0 15.917317584301772 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGCTATC 50 0.001614204 37.99938 7 CATTCCG 120 1.5002843E-7 31.666143 9 CTATACC 95 3.4723183E-5 30.013573 4 GCGCACT 40 4.4959853E-4 29.682375 92-93 CCTATAC 70 0.008364202 27.15514 3 CGCACTA 35 0.008395908 27.13817 92-93 GTTCTAA 125 7.1492996E-6 26.70386 1 GGTATCA 3475 0.0 26.072626 1 TACATGG 1800 0.0 25.080786 2 GTACATG 1870 0.0 24.480236 1 GTACCCA 100 0.0016140059 23.842731 1 GTAAGCG 70 1.7620448E-5 23.77747 94-95 CCTTAGA 100 0.0016467052 23.760746 2 CATGGGG 735 0.0 23.275833 4 ACATGGG 1940 0.0 23.025877 3 GACGAGG 125 3.765308E-10 22.82637 94-95 GACGTGA 125 2.2512188E-4 22.799625 7 TAGGCAT 190 3.2573735E-7 22.51018 5 ATGGGGT 260 6.166374E-10 21.932997 5 CATGGGT 310 1.0913936E-11 21.461319 4 >>END_MODULE