FastQCFastQC Report
Thu 26 May 2016
SRR938296_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938296_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences320189
Sequences flagged as poor quality0
Sequence length101
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37151.1602522260290016No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35381.1049723756906076No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA33811.0559388361249138No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24030.7504942393398899No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT17110.5343718866044742No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT13090.40882104007320674No Hit
ATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT11120.34729487896211303TruSeq Adapter, Index 11 (95% over 21bp)
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9010.2813963003101293No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8800.27483767399879444No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT8310.25953421260567977No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7570.23642286274669025No Hit
ACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCT7330.22892728981945037RNA PCR Primer, Index 11 (95% over 23bp)
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT5800.18114301240829633No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC4250.1327341039198723No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3960.1236769532994575No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC3810.1189922202199326No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG3730.11649369591085264No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3640.11368285606313772No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA3630.11337054052450272No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC3590.11212127836996275No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT3530.11024738513815278No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC3470.10837349190634282No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTACGG250.00501624557.0049672
CGTTGAA856.399592E-427.9392479
TAGAGTA700.00838551427.1409824
GGTATCA34650.026.8836231
GTGGTAG950.001220658925.0138991
CTCCGAC2000.023.74836294-95
CATTCCG1000.001651713423.7483629
GTATCAA45700.022.4632471
GTGTAGC1754.0147625E-621.726361
GAGTACT30400.021.32665312-13
GAGTCAA1604.9775204E-520.7798169
GTACTTT31300.020.40993514-15
TACATGG19600.020.116552
TATCAAC51750.019.9196112
GTACATG20050.019.6742741
GTAGCCC1456.1356206E-419.6538163
ACAGGGC1456.1356206E-419.6538168
AGTACTT29500.019.64271212-13
ATCAACG53250.019.2662753
ACATGGG20550.019.183593
Produced by FastQC (version 0.11.5)