##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938296_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 320189 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.61159502668736 31.0 31.0 34.0 30.0 34.0 2 31.86294969533619 31.0 31.0 34.0 30.0 34.0 3 31.869199129264278 31.0 31.0 34.0 30.0 34.0 4 35.582743317228264 37.0 35.0 37.0 33.0 37.0 5 35.48991376967978 37.0 35.0 37.0 33.0 37.0 6 35.552280059589805 37.0 35.0 37.0 33.0 37.0 7 35.46165545974409 37.0 35.0 37.0 33.0 37.0 8 35.470428403224346 37.0 35.0 37.0 33.0 37.0 9 36.912061313786545 39.0 37.0 39.0 33.0 39.0 10-11 36.878851240985796 39.0 37.0 39.0 32.5 39.0 12-13 36.77542170405604 39.0 37.0 39.0 32.0 39.0 14-15 37.63384126250433 39.5 36.5 41.0 32.0 41.0 16-17 37.70155595601348 39.0 36.5 41.0 32.0 41.0 18-19 37.901448831783725 40.0 37.0 41.0 33.0 41.0 20-21 37.9398261651712 40.0 37.0 41.0 33.0 41.0 22-23 37.78207714818436 40.0 37.0 41.0 32.0 41.0 24-25 37.63217349752802 39.5 36.0 41.0 32.0 41.0 26-27 37.345372576821816 39.0 36.0 41.0 32.0 41.0 28-29 37.1575975439506 39.0 36.0 41.0 31.5 41.0 30-31 36.77212833670114 39.0 35.0 40.0 31.0 41.0 32-33 36.42029551296265 38.0 35.0 40.0 30.0 41.0 34-35 36.14486756259584 38.0 35.0 40.0 30.0 41.0 36-37 35.90649741246577 38.0 35.0 40.0 30.0 41.0 38-39 35.582382592781144 38.0 35.0 40.0 29.0 41.0 40-41 35.28604199394732 38.0 34.5 40.0 27.5 41.0 42-43 34.8701563763902 38.0 34.0 40.0 25.5 41.0 44-45 34.34978715696042 38.0 33.5 40.0 23.5 41.0 46-47 33.6257725905637 38.0 33.0 40.0 20.0 41.0 48-49 32.97122636942556 38.0 33.0 40.0 11.5 41.0 50-51 32.455983809562476 37.0 32.0 40.0 4.5 41.0 52-53 31.844965005043896 37.0 31.0 40.0 2.0 41.0 54-55 31.390146132440528 36.0 30.5 39.5 2.0 41.0 56-57 30.949437675872687 36.0 30.0 39.0 2.0 40.0 58-59 30.372423787200685 35.5 28.5 39.0 2.0 40.0 60-61 29.831989855991306 35.0 28.0 39.0 2.0 40.0 62-63 29.35817907548354 35.0 26.5 38.5 2.0 40.0 64-65 28.70756646855451 34.0 26.0 38.0 2.0 40.0 66-67 28.026009638057523 34.0 24.5 38.0 2.0 40.0 68-69 27.30592556271452 33.5 22.5 37.0 2.0 39.0 70-71 26.58142066092214 32.5 21.0 36.0 2.0 39.0 72-73 26.044469047968544 32.0 19.0 36.0 2.0 39.0 74-75 25.401701807370024 31.5 17.5 35.0 2.0 38.0 76-77 24.323691632129773 30.5 15.0 34.0 2.0 36.0 78-79 24.77626651758805 31.0 12.5 35.0 2.0 37.0 80-81 24.421330838973233 31.0 7.5 35.0 2.0 36.0 82-83 23.79210403855223 31.0 4.5 34.0 2.0 36.0 84-85 23.107822254980654 30.0 2.0 34.0 2.0 35.5 86-87 22.303487939935476 29.5 2.0 34.0 2.0 35.0 88-89 21.436123664460677 29.0 2.0 34.0 2.0 35.0 90-91 20.640376465150272 28.0 2.0 33.5 2.0 35.0 92-93 19.676531673480348 26.5 2.0 33.0 2.0 35.0 94-95 18.63307921259006 25.0 2.0 32.5 2.0 34.0 96-97 17.36554035272917 22.0 2.0 32.0 2.0 34.0 98-99 15.748109710202412 2.0 2.0 32.0 2.0 34.0 100-101 11.946002204947703 2.0 2.0 27.0 2.0 31.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2995325635450925 1101 2 0.23941064021033043 1101 3 0.45409013377931373 1101 4 0.34260327033639015 1101 5 0.16822710362261972 1101 6 0.19336178552092775 1101 7 0.2493964408013909 1101 8 0.39985128909297885 1101 9 0.3050868683739125 1101 10-11 0.36344017319623134 1101 12-13 0.3213854639690936 1101 14-15 0.4789102286280027 1101 16-17 0.4390449189831429 1101 18-19 0.49079146896950476 1101 20-21 0.5856609306597989 1101 22-23 0.3859094141252015 1101 24-25 0.3064107021707869 1101 26-27 0.4249597193615884 1101 28-29 0.4694229393726346 1101 30-31 0.9438187593636869 1101 32-33 0.6876771434376678 1101 34-35 0.5736239908477145 1101 36-37 0.697087426060989 1101 38-39 0.6759064033257189 1101 40-41 0.3801938795720261 1101 42-43 0.47937579778704986 1101 44-45 0.7082656344678355 1101 46-47 0.3644346121406201 1101 48-49 0.7537193333149048 1101 50-51 0.9125025344207529 1101 52-53 1.1494683389815243 1101 54-55 1.4438351696128642 1101 56-57 1.3615095498910996 1101 58-59 1.3235152972631106 1101 60-61 1.41412986362662 1101 62-63 0.753046254499548 1101 64-65 1.279168697638596 1101 66-67 1.2525830583271897 1101 68-69 1.3367570109184008 1101 70-71 1.063417118792529 1101 72-73 1.504576362646521 1101 74-75 1.6531755821477745 1101 76-77 1.7408150641209552 1101 78-79 1.6734072266714684 1101 80-81 1.664290962074908 1101 82-83 1.8956969216373203 1101 84-85 1.8288820015007303 1101 86-87 1.6739261907233072 1101 88-89 1.6934249687202794 1101 90-91 2.152930839855177 1101 92-93 2.1921581837786945 1101 94-95 2.6067241124199114 1101 96-97 2.5469707537998616 1101 98-99 3.072529405079697 1101 100-101 2.8677388830510004 1102 1 0.34602838959828475 1102 2 0.5487356903624061 1102 3 0.43768555214467497 1102 4 0.20599549191148725 1102 5 0.3317875773170442 1102 6 0.355919217734737 1102 7 0.4157071895233244 1102 8 0.34536348737162825 1102 9 0.28340822992183234 1102 10-11 0.5176820679739436 1102 12-13 0.38871866787771836 1102 14-15 0.3093800403150553 1102 16-17 0.3868705839025779 1102 18-19 0.6439494318246091 1102 20-21 0.5347161302974044 1102 22-23 0.4987354026063997 1102 24-25 0.8984640251501474 1102 26-27 0.688290464200783 1102 28-29 0.7376880011729412 1102 30-31 0.6700855036851792 1102 32-33 0.7843088654333954 1102 34-35 0.819903030516393 1102 36-37 1.1893866559839807 1102 38-39 0.9154553582212088 1102 40-41 1.030520676957643 1102 42-43 0.986138238737027 1102 44-45 1.0084824208523457 1102 46-47 1.216324212865402 1102 48-49 1.220263046509757 1102 50-51 0.5182101640072361 1102 52-53 0.7651902052858084 1102 54-55 0.6644072268185859 1102 56-57 0.5251509140275168 1102 58-59 1.030465404038491 1102 60-61 0.33992097214923334 1102 62-63 0.5965556054346415 1102 64-65 1.0134597738050353 1102 66-67 1.2376183265598932 1102 68-69 1.3621889658786017 1102 70-71 1.8406286634764086 1102 72-73 1.7862045254628036 1102 74-75 2.0962448890784664 1102 76-77 1.835586305951022 1102 78-79 1.499411885960928 1102 80-81 1.712744336824148 1102 82-83 1.6866760195471109 1102 84-85 1.4426786752857552 1102 86-87 1.6535094431662003 1102 88-89 1.7071601657693982 1102 90-91 1.852681697293864 1102 92-93 2.5332408214542212 1102 94-95 2.151990403754926 1102 96-97 2.30268161900403 1102 98-99 2.4088718628548857 1102 100-101 1.8108567242468858 1103 1 0.17851707738109113 1103 2 0.10543755059367754 1103 3 0.298771524995356 1103 4 0.07014833253492014 1103 5 0.007292856351739374 1103 6 0.16704038415002742 1103 7 0.18334922069476534 1103 8 0.25645448787439307 1103 9 0.5123272847232343 1103 10-11 0.22380574821526977 1103 12-13 0.261679954956648 1103 14-15 0.36426691814310885 1103 16-17 0.1362370997396738 1103 18-19 0.3544421417290877 1103 20-21 0.2990348834396954 1103 22-23 0.17518588023335013 1103 24-25 0.36405477427030064 1103 26-27 0.24572133398459073 1103 28-29 0.08015155069530522 1103 30-31 0.19430873143781469 1103 32-33 0.32718971007894027 1103 34-35 0.37152193448823567 1103 36-37 0.5859678525651759 1103 38-39 -0.16138224861639827 1103 40-41 -0.24339586401772095 1103 42-43 -0.4616244560844933 1103 44-45 -0.3947503594392572 1103 46-47 -0.36846713957300636 1103 48-49 -0.1536937156823015 1103 50-51 -0.6704474528856679 1103 52-53 -0.5602346313154989 1103 54-55 -0.95114832873697 1103 56-57 -0.4963020774254723 1103 58-59 -0.3393686834426539 1103 60-61 -0.3587668106561921 1103 62-63 0.0827949216739583 1103 64-65 0.029678979436006614 1103 66-67 0.10720605908880287 1103 68-69 -0.20046563442305754 1103 70-71 0.23643057398420098 1103 72-73 0.2409053801636567 1103 74-75 -0.26811408528050507 1103 76-77 -0.12072339741750682 1103 78-79 -0.28539957708582975 1103 80-81 0.08653518649734693 1103 82-83 0.16223157510266972 1103 84-85 0.6161427275733367 1103 86-87 0.7842384527187392 1103 88-89 0.9421073754224878 1103 90-91 1.4716208627036131 1103 92-93 1.777459021554776 1103 94-95 1.9782448029505026 1103 96-97 1.317171312317253 1103 98-99 1.2804857391746456 1103 100-101 1.7319125311850456 1104 1 0.1674890839677765 1104 2 0.23399083217447725 1104 3 0.27929375505886966 1104 4 0.087508953565262 1104 5 0.2061354816163785 1104 6 0.2818651312683471 1104 7 0.12746589912337214 1104 8 0.3558569814053314 1104 9 0.17949265757668087 1104 10-11 0.1633917279847381 1104 12-13 0.25053434215966064 1104 14-15 0.350439583398348 1104 16-17 0.3374462270038521 1104 18-19 0.27498319089399104 1104 20-21 0.5498417524117016 1104 22-23 0.23360978049210956 1104 24-25 0.17444291823643 1104 26-27 -0.1862523192734713 1104 28-29 -0.11708204140066414 1104 30-31 -0.20938922198491383 1104 32-33 -0.2142969989307062 1104 34-35 -0.21111273970606703 1104 36-37 -0.4691250667479281 1104 38-39 -0.2601307783740978 1104 40-41 -0.05252783296620578 1104 42-43 -0.08467785528468141 1104 44-45 -0.5457689433018729 1104 46-47 -0.1174438509350395 1104 48-49 -0.5166200859481194 1104 50-51 -0.25333148017336526 1104 52-53 -0.5254945701534197 1104 54-55 -0.17708105044950884 1104 56-57 -0.8400376690509752 1104 58-59 -0.5400508758679656 1104 60-61 -0.38912202119489336 1104 62-63 -0.09890820700164582 1104 64-65 0.02854042380203836 1104 66-67 -0.09644955135579991 1104 68-69 0.08219256364798255 1104 70-71 -0.0677331521978708 1104 72-73 0.028814107521927923 1104 74-75 0.25450176981241057 1104 76-77 0.5649740855276839 1104 78-79 0.19346657205554862 1104 80-81 0.4352813638673787 1104 82-83 0.45476980140236023 1104 84-85 0.3658063361360284 1104 86-87 0.18469011115205092 1104 88-89 -0.08601306682639276 1104 90-91 0.2064173811698602 1104 92-93 0.3067462480897092 1104 94-95 0.6092069295089608 1104 96-97 0.39094689453320086 1104 98-99 0.32594327857714056 1104 100-101 0.4984803975694252 1105 1 -0.05015508379106848 1105 2 0.06437527953140432 1105 3 -0.13286766914383819 1105 4 -0.085437748051163 1105 5 0.00318113474001791 1105 6 0.006362728472367962 1105 7 -0.030954808609259032 1105 8 0.12483360875351934 1105 9 -0.12339615850020635 1105 10-11 0.1973039167134445 1105 12-13 0.15663416741086422 1105 14-15 0.3430810207072099 1105 16-17 0.10386988362246541 1105 18-19 0.2691536801906267 1105 20-21 0.050005579410388634 1105 22-23 -1.84998887526433E-4 1105 24-25 -0.0422455920300564 1105 26-27 0.16862426438752465 1105 28-29 0.4425782906220519 1105 30-31 0.42183620396529165 1105 32-33 0.13042231081154654 1105 34-35 0.01105032276662854 1105 36-37 -0.05118874084141822 1105 38-39 0.0935307550466078 1105 40-41 0.2700748319529751 1105 42-43 0.45783525087522037 1105 44-45 0.19049689330800135 1105 46-47 0.05330483844897316 1105 48-49 0.48403522204664284 1105 50-51 0.4803950379568178 1105 52-53 0.3221463210654498 1105 54-55 0.38861357602493385 1105 56-57 0.6809048822814852 1105 58-59 0.5455214264474542 1105 60-61 0.5680372186478344 1105 62-63 0.8162976689267047 1105 64-65 1.0250727523297805 1105 66-67 1.2990970847298264 1105 68-69 1.0677211787637582 1105 70-71 1.069649438453066 1105 72-73 1.5505894460977245 1105 74-75 1.682554412887992 1105 76-77 1.6530174450733348 1105 78-79 1.8246919980057434 1105 80-81 1.6482018531640144 1105 82-83 2.304731575102668 1105 84-85 2.0566784418590522 1105 86-87 1.6464774637077504 1105 88-89 1.7804269724920871 1105 90-91 1.692696870029625 1105 92-93 2.2697896076353636 1105 94-95 1.9173382095439102 1105 96-97 1.8006099570058964 1105 98-99 1.9523767648156696 1105 100-101 1.738377732650246 1106 1 0.2586736872383 1106 2 0.09096495731868615 1106 3 0.1830184158290713 1106 4 0.22597514045753542 1106 5 0.29351118891737116 1106 6 -0.0052212464349565835 1106 7 0.07636626353217224 1106 8 -0.04513003538677651 1106 9 -0.19056539146285445 1106 10-11 -0.03861709839919314 1106 12-13 0.16991118480464706 1106 14-15 0.4058928969547182 1106 16-17 0.13871982659365756 1106 18-19 0.3569156562385274 1106 20-21 0.11733520587174695 1106 22-23 0.004439680325468487 1106 24-25 0.1409594265042955 1106 26-27 -0.20083324915682255 1106 28-29 0.14683050048802926 1106 30-31 -0.006414437608704304 1106 32-33 0.014172667241538761 1106 34-35 -0.1661934040654245 1106 36-37 -0.11932924554629665 1106 38-39 0.0450295431846186 1106 40-41 -0.4062332661365602 1106 42-43 0.0029771100140862927 1106 44-45 0.16820219698635697 1106 46-47 -0.4204708245108222 1106 48-49 -0.2226941762995267 1106 50-51 0.4786128879710745 1106 52-53 0.17066357227731643 1106 54-55 0.17022891677201102 1106 56-57 0.9077191109669727 1106 58-59 1.212865310108704 1106 60-61 0.9206712363267613 1106 62-63 1.1572675149489449 1106 64-65 1.5830460913659898 1106 66-67 1.7077173442108666 1106 68-69 1.1878024448031077 1106 70-71 1.5415894869275455 1106 72-73 1.9397308062345964 1106 74-75 1.5223925936692844 1106 76-77 1.7483000940610758 1106 78-79 2.001730777589433 1106 80-81 1.6858765038626196 1106 82-83 1.6733692996535687 1106 84-85 1.686142727573337 1106 86-87 2.159282211355311 1106 88-89 2.08543303180204 1106 90-91 2.0345043265451643 1106 92-93 2.160231937261827 1106 94-95 2.0836662677132836 1106 96-97 1.918401160313568 1106 98-99 2.543631754835628 1106 100-101 3.0747407209593423 1107 1 -0.14715871575232953 1107 2 -0.02581842506907961 1107 3 -0.0948834076426266 1107 4 -0.1365878691165392 1107 5 0.05761010971983893 1107 6 -0.0336897331902648 1107 7 0.10574817766999445 1107 8 0.19802771690525134 1107 9 0.12493919258938035 1107 10-11 0.0014806721613851437 1107 12-13 0.02270559598228772 1107 14-15 0.04982537098970141 1107 16-17 -0.07059036657931017 1107 18-19 0.13973983838271664 1107 20-21 0.0051105027356541655 1107 22-23 0.09613764033765193 1107 24-25 0.1661886291160215 1107 26-27 0.24091038222449157 1107 28-29 0.3649451187092154 1107 30-31 -0.05183207690638625 1107 32-33 -0.03215839943865717 1107 34-35 0.5443685229280462 1107 36-37 0.5980495561722989 1107 38-39 0.3909732489933404 1107 40-41 0.47482664793360385 1107 42-43 0.5406137012384136 1107 44-45 0.9292479021861269 1107 46-47 0.75717388606801 1107 48-49 1.3030424859691578 1107 50-51 1.1607824471577928 1107 52-53 0.9658105664246115 1107 54-55 0.8786256825624612 1107 56-57 1.030329821748797 1107 58-59 0.7643470923070197 1107 60-61 0.7035799950804424 1107 62-63 0.9590791459242816 1107 64-65 0.9381579016437449 1107 66-67 0.5030165762552485 1107 68-69 1.062172349062383 1107 70-71 0.9703506087516942 1107 72-73 1.0936433201897344 1107 74-75 1.1047194653496568 1107 76-77 1.0176512223130452 1107 78-79 0.7055434911453737 1107 80-81 1.0472131525990456 1107 82-83 1.150423665498149 1107 84-85 1.2030918801157107 1107 86-87 1.899327544418 1107 88-89 1.595827497108715 1107 90-91 1.507744287301616 1107 92-93 2.119592876400496 1107 94-95 1.6829253766141257 1107 96-97 2.0532078181842657 1107 98-99 2.0192906066235796 1107 100-101 2.0120863686470187 1108 1 0.0748783565513591 1108 2 0.10142717894285624 1108 3 0.12258310667210637 1108 4 -0.035210353722646914 1108 5 0.17536545790748193 1108 6 -0.022596608071239643 1108 7 -0.10525924948673548 1108 8 0.10426779815961851 1108 9 0.2848769822167583 1108 10-11 0.24498984501735066 1108 12-13 0.11493406040176524 1108 14-15 0.043104428946911355 1108 16-17 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66-67 -0.8542793520517833 2316 68-69 -0.21390667613995973 2316 70-71 -0.36581201101700245 2316 72-73 -0.2667386998942156 2316 74-75 -0.07120547664775856 2316 76-77 0.12326020518678504 2316 78-79 0.2686201962442887 2316 80-81 0.10183299213998254 2316 82-83 0.45539771930329564 2316 84-85 0.5474279940310147 2316 86-87 0.1076143871291535 2316 88-89 0.40005770095902093 2316 90-91 0.3686886225299979 2316 92-93 0.43190802083136504 2316 94-95 0.17111675858107844 2316 96-97 -0.57585014898007 2316 98-99 -0.40319402933270965 2316 100-101 -0.18815981026660644 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 2.0 4 7.0 5 36.0 6 89.0 7 165.0 8 368.0 9 694.0 10 1114.0 11 1545.0 12 2216.0 13 2813.0 14 3632.0 15 5092.0 16 6172.0 17 7939.0 18 7181.0 19 5424.0 20 5172.0 21 5203.0 22 5441.0 23 6008.0 24 6381.0 25 6860.0 26 7134.0 27 8220.0 28 9719.0 29 11385.0 30 13279.0 31 15153.0 32 17803.0 33 21366.0 34 26220.0 35 31327.0 36 35964.0 37 32911.0 38 10002.0 39 152.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.332925137766004 22.467104852112413 16.114248940982417 20.085721069139165 2 15.768678160919542 23.800912043978013 38.92866066966516 21.501749125437282 3 19.37717451731181 27.401260517324307 32.42802620976558 20.79353875559831 4 12.220282395710033 21.202164971313817 36.628366371112065 29.949186261864085 5 11.234025010306445 40.172336252451686 34.6661961097855 13.927442627456369 6 26.855076220607206 39.10253006817848 17.690801370440585 16.35159234077373 7 23.379628906676995 37.321082235804475 20.950750962712647 18.34853789480588 8 24.472108660822204 35.05960542054849 20.43667958611945 20.03160633250986 9 24.54128368734521 18.06975298809758 22.058359646081822 35.33060367847539 10-11 23.165224289404073 28.711011308945654 27.370084543816308 20.75367985783397 12-13 25.363769523625106 27.661787256901395 27.509064958508883 19.465378260964616 14-15 21.773858564785172 29.135448125950607 28.10308911299264 20.98760419627158 16-17 18.468467061641718 31.807932190050252 29.234295993928587 20.489304754379447 18-19 17.941278432425847 29.594083494436095 33.35045863536848 19.114179437769568 20-21 19.657608474994458 27.959111649681905 33.433222253106756 18.950057622216878 22-23 18.82513140676288 29.201502862371914 33.070155439443575 18.90321029142163 24-25 18.984924246446564 29.28528372836784 33.6855798090467 18.044212216138895 26-27 18.787880680833254 29.05660023768883 34.18408859140478 17.971430490073136 28-29 17.507343330569334 29.86381459671412 33.94968901131677 18.67915306139977 30-31 18.655700227053394 29.18791713644129 34.03848976698138 18.11789286952394 32-33 17.839363124164553 29.714105463040465 34.07466863217859 18.37186278061639 34-35 18.425248743981495 29.923147441033116 33.84745368309759 17.8041501318878 36-37 18.463856832768556 29.229351781001604 33.68162156253416 18.625169823695675 38-39 18.01537709490663 29.40951393324723 33.912694458717205 18.66241451312894 40-41 18.51735587269757 29.206686844645187 33.80373636190422 18.472220920753017 42-43 18.19487909850146 29.269500090574617 33.314593757222546 19.221027053701377 44-45 18.748301784258672 29.27692706495226 32.72239208717351 19.252379063615553 46-47 19.385737798612695 29.126390975330196 32.00016240408009 19.48770882197702 48-49 19.035475922033548 29.011615014881837 31.997351564232375 19.95555749885224 50-51 18.44473108070546 29.521938605011417 31.93785545412241 20.095474860160717 52-53 18.922730012586317 29.895624146988187 31.327434733860315 19.854211106565185 54-55 18.67100368844651 29.25084871747624 31.093354237653386 20.98479335642386 56-57 19.5286221575382 29.56987904019189 30.24213823710371 20.6593605651662 58-59 19.292667768099466 29.884849260905277 30.321622541686317 20.50086042930894 60-61 19.034226659879007 30.10862334433725 30.430932980208564 20.426217015575176 62-63 18.58558538862984 30.131422378657607 30.3428599983135 20.94013223439906 64-65 18.348850210344516 30.403293055039367 30.042724765685268 21.20513196893085 66-67 18.61869083572515 30.148287417743898 29.491643997763823 21.741377748767135 68-69 18.64382164769595 29.43065994350168 29.79685277174397 22.1286656370584 70-71 18.985036962543997 28.992719924794418 29.74118411313318 22.2810589995284 72-73 18.848555072160504 28.411969180702645 30.27883531289332 22.460640434243526 74-75 18.467530115025813 29.15340626942212 29.445421298045844 22.933642317506223 76-77 18.30606298155152 28.322334621114404 30.343484629390765 23.02811776794331 78-79 18.109928823288747 28.548763386624778 30.1278307499633 23.213477040123177 80-81 18.370897143401464 28.144046397668877 30.044020943912724 23.441035515016935 82-83 18.041175937811342 28.23115866665417 30.282928413708238 23.444736981826246 84-85 17.978365058022685 28.086383604043398 29.903662511146194 24.031588826787726 86-87 17.86853143358642 28.284449830253322 29.601665297657902 24.245353438502356 88-89 18.11836134283189 28.058584148737154 29.04315888428397 24.779895624146988 90-91 18.08874786063188 27.441065873799143 29.55576099041813 24.914425275150847 92-93 18.055329993628742 27.695447674491234 29.49220457980936 24.75701775207066 94-95 17.766850204098205 27.389604264980992 29.549890845719247 25.293654685201556 96-97 17.716879717916605 27.42676981407856 29.43933114504246 25.417019322962375 98-99 18.190506232256574 27.199247944182968 28.777690676444223 25.83255514711623 100-101 17.878659166929534 26.81275746512216 29.312999509664607 25.995583858283698 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2294.0 1 1376.0 2 453.0 3 425.0 4 348.0 5 355.5 6 589.0 7 1000.0 8 1303.0 9 1344.0 10 1041.0 11 674.0 12 592.5 13 589.0 14 606.0 15 640.5 16 661.0 17 724.5 18 875.5 19 988.5 20 1074.0 21 1223.0 22 1431.5 23 1712.0 24 2013.5 25 2411.5 26 3028.0 27 3788.5 28 4359.5 29 4952.0 30 5655.0 31 6338.0 32 6973.0 33 7618.5 34 8744.5 35 9997.5 36 10916.5 37 11259.5 38 10985.5 39 10751.0 40 10764.0 41 10719.0 42 10795.5 43 11081.5 44 11360.0 45 11696.0 46 12030.0 47 12771.0 48 14117.0 49 14025.0 50 12337.0 51 10570.0 52 8766.0 53 7335.5 54 6195.0 55 5182.5 56 4368.5 57 3557.0 58 2829.0 59 2205.5 60 1660.5 61 1241.0 62 872.5 63 588.5 64 420.5 65 305.5 66 259.5 67 229.0 68 193.5 69 151.5 70 109.0 71 93.0 72 77.0 73 62.5 74 59.0 75 55.0 76 46.0 77 34.5 78 26.5 79 17.5 80 9.0 81 4.5 82 2.0 83 0.0 84 0.0 85 0.0 86 0.5 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02529755862943449 2 0.009057150620414818 3 9.369466159049812E-4 4 0.0 5 3.123155386349937E-4 6 0.0 7 0.0 8 0.0 9 6.246310772699874E-4 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0018738932318099625 26-27 0.0070270996192873586 28-29 1.5615776931749686E-4 30-31 0.0 32-33 3.123155386349937E-4 34-35 0.009525623928367309 36-37 0.001249262154539975 38-39 0.011711832698812263 40-41 0.01186799046812976 42-43 0.0034354709249849306 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 7.807888465874842E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 1.5615776931749686E-4 82-83 6.246310772699874E-4 84-85 0.0039039442329374214 86-87 0.0018738932318099625 88-89 0.0 90-91 3.123155386349937E-4 92-93 3.123155386349937E-4 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 320189.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.176621836860654 #Duplication Level Percentage of deduplicated Percentage of total 1 77.87686214266894 46.08489620858049 2 13.25169448385018 15.683810263368287 3 4.375315908015193 7.7674924471624704 4 1.8248016187951754 4.31942381290933 5 0.9236470633895079 2.7329156490463884 6 0.5228995315433737 1.8566056702088285 7 0.27977178792384116 1.1589164506214067 8 0.1783916127463211 0.8445290405085386 9 0.13213353037722875 0.7037294363192345 >10 0.5404154479412862 5.76626559458568 >50 0.05126116099238053 2.0912126454284374 >100 0.03593565925239044 4.156781588609954 >500 0.003170793463446215 1.464192852683705 >1k 0.0036992590406872508 5.369228339967221 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3715 1.1602522260290016 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3538 1.1049723756906076 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3381 1.0559388361249138 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2403 0.7504942393398899 No Hit CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT 1711 0.5343718866044742 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1309 0.40882104007320674 No Hit ATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT 1112 0.34729487896211303 TruSeq Adapter, Index 11 (95% over 21bp) GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 901 0.2813963003101293 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 880 0.27483767399879444 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 831 0.25953421260567977 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 757 0.23642286274669025 No Hit ACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCT 733 0.22892728981945037 RNA PCR Primer, Index 11 (95% over 23bp) GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 580 0.18114301240829633 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 425 0.1327341039198723 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 396 0.1236769532994575 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 381 0.1189922202199326 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 373 0.11649369591085264 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 364 0.11368285606313772 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 363 0.11337054052450272 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 359 0.11212127836996275 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 353 0.11024738513815278 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 347 0.10837349190634282 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 3.123155386349937E-4 0.0 14-15 0.0 0.0 0.0 3.123155386349937E-4 0.0 16-17 0.0 0.0 0.0 3.123155386349937E-4 0.0 18-19 0.0 0.0 0.0 3.123155386349937E-4 0.0 20-21 0.0 0.0 0.0 0.001093104385222478 0.0 22-23 0.0 0.0 0.0 0.005777837464747384 0.0 24-25 0.0 0.0 0.0 0.014054199238574715 0.0 26-27 0.0 0.0 0.0 0.027171451861244453 0.0 28-29 0.0 0.0 0.0 0.05434290372248891 0.0 30-31 0.0 0.0 0.0 0.12726858199375995 0.0 32-33 0.0 0.0 0.0 0.24641695998301003 0.0 34-35 0.0 0.0 0.0 0.4344309142412762 0.0 36-37 0.0 0.0 0.0 0.7255089962490904 0.0 38-39 0.0 0.0 0.0 1.0712422975180285 0.0 40-41 0.0 0.0 0.0 1.3810593118439423 0.0 42-43 0.0 0.0 0.0 1.6722935516210737 0.0 44-45 0.0 0.0 0.0 2.0383273629012866 0.0 46-47 0.0 0.0 0.0 2.4538631870551457 0.0 48-49 0.0 0.0 0.0 2.850660078890905 0.0 50-51 0.0 0.0 0.0 3.2290303539472003 0.0 52-53 0.0 0.0 0.0 3.599905056076255 0.0 54-55 0.0 0.0 0.0 4.031056657161864 0.0 56-57 0.0 0.0 0.0 4.526701416975598 0.0 58-59 0.0 0.0 0.0 5.033121062872241 0.0 60-61 0.0 0.0 0.0 5.565306740706271 0.0 62-63 0.0 3.123155386349937E-4 0.0 6.055642136363211 0.0 64-65 0.0 3.123155386349937E-4 0.0 6.5773652436529675 0.0 66-67 0.0 3.123155386349937E-4 0.0 7.2104288404661 0.0 68-69 0.0 3.123155386349937E-4 0.0 7.897210709924451 0.0 70-71 0.0 3.123155386349937E-4 0.0 8.615848764323571 0.0 72-73 0.0 3.123155386349937E-4 0.0 9.315591728635274 0.0 74-75 0.0 3.123155386349937E-4 0.0 10.021893319258313 0.0 76-77 0.0 3.123155386349937E-4 0.0 10.78628560006746 0.0 78-79 0.0 3.123155386349937E-4 0.0 11.629849869920578 0.0 80-81 0.0 3.123155386349937E-4 0.0 12.492309229861114 0.0 82-83 0.0 3.123155386349937E-4 0.0 13.33275034432788 0.0 84-85 0.0 3.123155386349937E-4 0.0 14.178032349643491 0.0 86-87 0.0 3.123155386349937E-4 0.0 15.023314354959103 0.0 88-89 0.0 3.123155386349937E-4 0.0 15.923095421766519 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTTACGG 25 0.005016245 57.004967 2 CGTTGAA 85 6.399592E-4 27.939247 9 TAGAGTA 70 0.008385514 27.140982 4 GGTATCA 3465 0.0 26.883623 1 GTGGTAG 95 0.0012206589 25.013899 1 CTCCGAC 200 0.0 23.748362 94-95 CATTCCG 100 0.0016517134 23.748362 9 GTATCAA 4570 0.0 22.463247 1 GTGTAGC 175 4.0147625E-6 21.72636 1 GAGTACT 3040 0.0 21.326653 12-13 GAGTCAA 160 4.9775204E-5 20.779816 9 GTACTTT 3130 0.0 20.409935 14-15 TACATGG 1960 0.0 20.11655 2 TATCAAC 5175 0.0 19.919611 2 GTACATG 2005 0.0 19.674274 1 GTAGCCC 145 6.1356206E-4 19.653816 3 ACAGGGC 145 6.1356206E-4 19.653816 8 AGTACTT 2950 0.0 19.642712 12-13 ATCAACG 5325 0.0 19.266275 3 ACATGGG 2055 0.0 19.18359 3 >>END_MODULE