FastQCFastQC Report
Thu 26 May 2016
SRR938295_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938295_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2499311
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75000.30008270279288973No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56610.22650242406807314No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41430.16576568502279226No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA36650.050.7379721
GTATCAA61550.043.502141
ATCAACG80900.032.7430843
TATCAAC83100.032.1623842
TCAACGC83350.031.8947454
CAACGCA84650.031.4049225
AACGCAG87300.030.4278456
ACGCAGA103500.025.3872097
GTACATG75350.025.057491
CGCAGAG104850.024.9238838
TACATGG73600.024.7476432
TGGTATC17600.023.5082072
GTGGTAT19700.023.175831
ACATGGG77950.022.8175163
GCAGAGT114550.022.2736789
TAGGACG1553.8729042E-521.477284
GAGTACT69300.021.15118412-13
AGAGTAC105250.020.24705110-11
ATTCGCG3200.020.03212780-81
CGCGGAA3350.019.84685582-83
Produced by FastQC (version 0.11.5)