FastQCFastQC Report
Thu 26 May 2016
SRR938295_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938295_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2499311
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74110.2965217213864141No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64430.25779104721261176No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47280.18917213584063766No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA42250.055.007331
GTATCAA69450.040.717691
ATCAACG91750.030.6504363
TATCAAC93850.030.181562
TCAACGC94350.029.8058054
CAACGCA96150.029.1984145
AACGCAG99450.028.2171136
TGGTATC18300.028.048032
ACGCAGA116300.024.1289067
CGCAGAG116600.024.0261048
GTGGTAT21950.023.3844761
GAGTACT83000.021.30973412-13
GCAGAGT128800.021.2710939
GTACATG73900.021.2230551
TACATGG72350.021.0204092
AGGACCG10300.020.753745
GGACCGA9100.020.3494686
AGAGTAC118750.019.83252110-11
CAGAGTA124500.019.77467210-11
ACATGGG74650.019.7266313
Produced by FastQC (version 0.11.5)