##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938295_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2499311 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.359641117091872 31.0 31.0 34.0 30.0 34.0 2 31.622714420094177 31.0 31.0 34.0 30.0 34.0 3 31.670013455708393 31.0 31.0 34.0 30.0 34.0 4 35.42040946484851 37.0 35.0 37.0 33.0 37.0 5 35.303007908979716 37.0 35.0 37.0 33.0 37.0 6 35.312262059423574 37.0 35.0 37.0 32.0 37.0 7 35.24567770877654 37.0 35.0 37.0 32.0 37.0 8 35.21927483214374 37.0 35.0 37.0 32.0 37.0 9 36.72708278401527 39.0 35.0 39.0 32.0 39.0 10-11 36.587644354784175 38.0 35.0 39.0 32.0 39.0 12-13 36.44749993098098 38.0 35.0 39.0 32.0 39.0 14-15 37.31966409942581 39.0 36.0 40.0 32.0 41.0 16-17 37.17743550122414 39.0 36.0 40.0 32.0 41.0 18-19 37.45358800885524 39.0 36.0 40.5 32.0 41.0 20-21 37.41206436493898 39.0 36.0 40.5 32.0 41.0 22-23 37.2611525736493 39.0 36.0 40.0 31.5 41.0 24-25 37.092451679682924 39.0 36.0 40.0 31.0 41.0 26-27 36.82303222768195 39.0 36.0 40.0 30.5 41.0 28-29 36.66544639702702 38.5 35.5 40.0 30.0 41.0 30-31 36.35199280921822 38.0 35.0 40.0 30.0 41.0 32-33 36.10152898138727 38.0 35.0 40.0 30.0 41.0 34-35 35.95934699603211 38.0 35.0 40.0 29.0 41.0 36-37 35.882407991642495 38.0 35.0 40.0 29.0 41.0 38-39 35.66244256917206 38.0 34.0 40.0 28.5 41.0 40-41 35.428524301297436 38.0 34.0 40.0 27.0 41.0 42-43 35.1509898127924 38.0 34.0 40.0 27.0 41.0 44-45 34.77145981432483 38.0 33.0 40.0 25.5 41.0 46-47 34.28397626385832 37.5 33.0 40.0 24.0 41.0 48-49 33.825593133467585 37.0 32.5 40.0 23.5 41.0 50-51 33.32366760279133 37.0 31.5 39.5 22.5 40.5 52-53 32.667858461792065 36.0 31.0 39.0 19.5 40.0 54-55 32.18834470780147 36.0 30.0 39.0 18.0 40.0 56-57 31.719210414390204 35.0 30.0 39.0 14.5 40.0 58-59 31.086791119632572 35.0 28.5 38.0 11.0 40.0 60-61 30.53453751854011 34.0 28.0 38.0 8.0 40.0 62-63 29.895088486386847 34.0 27.5 38.0 7.5 40.0 64-65 29.183372137361054 33.5 26.0 37.0 4.0 39.0 66-67 28.507250198154612 33.0 26.0 36.5 2.0 39.0 68-69 27.823294700019325 32.0 25.0 36.0 2.0 39.0 70-71 27.036974190086788 31.5 23.5 35.5 2.0 38.0 72-73 26.34833760184307 31.0 22.0 35.0 2.0 38.0 74-75 25.596391565515454 31.0 20.0 34.5 2.0 37.0 76-77 24.667329115904344 29.5 20.0 33.5 2.0 36.0 78-79 25.245613491078142 31.0 20.0 34.0 2.0 36.0 80-81 24.67654025449414 30.5 18.0 34.0 2.0 36.0 82-83 24.024321903116498 30.0 14.5 34.0 2.0 35.5 84-85 23.215426371507988 29.5 7.0 34.0 2.0 35.0 86-87 22.31699816469419 29.0 2.0 34.0 2.0 35.0 88-89 21.259116612538417 28.0 2.0 33.0 2.0 35.0 90-91 20.335447849427304 26.5 2.0 33.0 2.0 34.0 92-93 19.16710065293995 25.5 2.0 32.0 2.0 34.0 94-95 18.001213134339825 24.0 2.0 32.0 2.0 34.0 96-97 16.603115018499096 18.5 2.0 31.5 2.0 34.0 98-99 14.986372644300769 2.0 2.0 31.0 2.0 34.0 100-101 11.893695502480483 2.0 2.0 27.0 2.0 32.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.30311594038837697 1101 2 0.4048309685408853 1101 3 0.47623890191108487 1101 4 0.3224276331183731 1101 5 0.2146335472516725 1101 6 0.34638614671135315 1101 7 0.3391545340315787 1101 8 0.30202874199208907 1101 9 0.3647065535111338 1101 10-11 0.3844513974638488 1101 12-13 0.48123403175531365 1101 14-15 0.6095517412228091 1101 16-17 0.5339664294640869 1101 18-19 0.4987032284070807 1101 20-21 0.5406133213413398 1101 22-23 0.5088771209171838 1101 24-25 0.6094907288629088 1101 26-27 0.559453969555797 1101 28-29 0.6053841049699002 1101 30-31 0.7209101070466133 1101 32-33 0.6069016100197118 1101 34-35 0.5989534335370195 1101 36-37 0.7158118963982432 1101 38-39 0.8623850465656062 1101 40-41 0.7601305560760565 1101 42-43 0.7671727499693404 1101 44-45 1.0928592049206358 1101 46-47 0.846033144499188 1101 48-49 0.9467531702636478 1101 50-51 0.7415610988762111 1101 52-53 0.8719158543769225 1101 54-55 0.8248651209046827 1101 56-57 0.8949797654651057 1101 58-59 1.164763241540843 1101 60-61 1.267985036286948 1101 62-63 1.4968574087280366 1101 64-65 1.4857049987230369 1101 66-67 1.3926881149065693 1101 68-69 1.523617880855781 1101 70-71 1.4058043972019618 1101 72-73 1.616451614603708 1101 74-75 1.6752665462687766 1101 76-77 1.6235272682102853 1101 78-79 1.840658019066531 1101 80-81 1.7784913641688824 1101 82-83 1.818982074915727 1101 84-85 2.193062786928934 1101 86-87 2.093047831313548 1101 88-89 2.364058544810323 1101 90-91 2.522467163505766 1101 92-93 2.591417936952663 1101 94-95 2.13182775494154 1101 96-97 2.355796018457113 1101 98-99 2.261708274607191 1101 100-101 2.0889052084815294 1102 1 0.34683465752648246 1102 2 0.4060670467323888 1102 3 0.3251462110059826 1102 4 0.3350917556025834 1102 5 0.2441869523673006 1102 6 0.21978481323175458 1102 7 0.20685926259855592 1102 8 0.2687272109394314 1102 9 0.38962231071013775 1102 10-11 0.4396441447709094 1102 12-13 0.4274128270583262 1102 14-15 0.529754195428076 1102 16-17 0.5125645749589651 1102 18-19 0.5632683682318458 1102 20-21 0.6155811035256349 1102 22-23 0.5446333558817429 1102 24-25 0.5878766744710333 1102 26-27 0.5817334340364155 1102 28-29 0.498319566285808 1102 30-31 0.6354927270448201 1102 32-33 0.6547285834509537 1102 34-35 0.5171336172810612 1102 36-37 0.6225587724059167 1102 38-39 0.6314057468584764 1102 40-41 0.5647430779747467 1102 42-43 0.5595153536120918 1102 44-45 0.6735321445040583 1102 46-47 0.7928161277304184 1102 48-49 0.8330656958190445 1102 50-51 0.9522368031526796 1102 52-53 0.8330421371335461 1102 54-55 0.7269066167864509 1102 56-57 1.0780099244432613 1102 58-59 1.2171159454549603 1102 60-61 1.0664517951469819 1102 62-63 1.1586343362288076 1102 64-65 1.2025242498513506 1102 66-67 1.1012609349267137 1102 68-69 1.312654903364482 1102 70-71 1.174914853060507 1102 72-73 1.279473623554889 1102 74-75 1.5883718007368834 1102 76-77 1.589624457974601 1102 78-79 1.7957282943501767 1102 80-81 1.9437953529727032 1102 82-83 1.988359830605507 1102 84-85 1.8507793831762633 1102 86-87 1.7956714363624613 1102 88-89 1.7934731335248308 1102 90-91 1.8271688869234346 1102 92-93 2.1160043133875064 1102 94-95 2.3009154671829783 1102 96-97 2.368768883465851 1102 98-99 2.6132745923352445 1102 100-101 2.4454783077412134 1103 1 0.2891879703557976 1103 2 0.28934912002233304 1103 3 0.2872107986606558 1103 4 0.2074035835595751 1103 5 0.15889858482086794 1103 6 0.20202559238440898 1103 7 0.30516529697855077 1103 8 0.30672818845653893 1103 9 0.26644025410951144 1103 10-11 0.33658612555435496 1103 12-13 0.4541425554993097 1103 14-15 0.6118000672064383 1103 16-17 0.40265946564394284 1103 18-19 0.584401215190816 1103 20-21 0.5824095969571985 1103 22-23 0.3972219880730492 1103 24-25 0.5074952639853763 1103 26-27 0.5033428205848267 1103 28-29 0.5565339882394085 1103 30-31 0.47978140453570006 1103 32-33 0.5902631184150735 1103 34-35 0.5852334789981626 1103 36-37 0.6598370429158962 1103 38-39 0.5836981436827386 1103 40-41 0.7258456457452951 1103 42-43 0.8843758786103066 1103 44-45 0.7786137433405713 1103 46-47 0.8655547253509965 1103 48-49 1.089480651830776 1103 50-51 1.2344352629671889 1103 52-53 1.1560787582622183 1103 54-55 1.075689488779389 1103 56-57 1.2202014105076806 1103 58-59 1.4223259924234704 1103 60-61 1.2100484620520717 1103 62-63 1.500196098620627 1103 64-65 1.537475850833637 1103 66-67 1.4555632022895644 1103 68-69 1.4345614193504659 1103 70-71 1.3171793548844093 1103 72-73 1.3723288079718365 1103 74-75 1.4208751386001772 1103 76-77 1.4595893508053486 1103 78-79 1.805710591753794 1103 80-81 1.9137143501832057 1103 82-83 2.1279048679180512 1103 84-85 2.039990038112723 1103 86-87 2.0776612797213083 1103 88-89 1.9483380692709638 1103 90-91 2.066362554519934 1103 92-93 2.4163315431993944 1103 94-95 2.328232301934907 1103 96-97 2.3669211377156643 1103 98-99 2.330095635179106 1103 100-101 2.1172873700400476 1104 1 0.2535506151373319 1104 2 0.30485577628090255 1104 3 0.2923126995446417 1104 4 0.2980631966315812 1104 5 0.20812852638081836 1104 6 0.12427975386461299 1104 7 0.275911515253199 1104 8 0.2526273635446188 1104 9 0.27312446685661484 1104 10-11 0.3572777540697203 1104 12-13 0.39773630220282286 1104 14-15 0.5295214326360878 1104 16-17 0.5529052514200146 1104 18-19 0.38161581887443674 1104 20-21 0.4276413001576813 1104 22-23 0.4877460497621158 1104 24-25 0.5810297794814758 1104 26-27 0.5335668232009141 1104 28-29 0.618984409134363 1104 30-31 0.6202416463129552 1104 32-33 0.8090979039590778 1104 34-35 0.8244334093764394 1104 36-37 0.7323926348060183 1104 38-39 0.8475955887765139 1104 40-41 0.9110821486251055 1104 42-43 1.0053008981254834 1104 44-45 1.045753081723234 1104 46-47 1.0167678311127162 1104 48-49 1.0116955192330437 1104 50-51 1.1371774094259735 1104 52-53 1.1609206325633608 1104 54-55 1.237526236390103 1104 56-57 0.9976711356073729 1104 58-59 1.2469256675220954 1104 60-61 1.3222913363743132 1104 62-63 1.2991960775231348 1104 64-65 1.4531022353565177 1104 66-67 1.4687348936754567 1104 68-69 1.637646927483548 1104 70-71 1.694964223763499 1104 72-73 1.510406615520516 1104 74-75 1.4113952445095812 1104 76-77 1.5426583501513242 1104 78-79 1.9982977243937228 1104 80-81 2.0639799148575655 1104 82-83 2.1617940322464158 1104 84-85 2.3060170112556193 1104 86-87 2.464815544579217 1104 88-89 2.489612674220435 1104 90-91 2.4523569847821776 1104 92-93 2.85396472111454 1104 94-95 2.6248772733478027 1104 96-97 2.5531049179641947 1104 98-99 2.592396527602993 1104 100-101 2.441757559284545 1105 1 0.17980960794308842 1105 2 0.2800356323960109 1105 3 0.2336796060194608 1105 4 0.2300775851207888 1105 5 0.15273284292757694 1105 6 0.1375891063825989 1105 7 0.27627122748341293 1105 8 0.17996549304102416 1105 9 0.29254892728827286 1105 10-11 0.3387525742136077 1105 12-13 0.428671554001383 1105 14-15 0.563514238391484 1105 16-17 0.4458908629308027 1105 18-19 0.42639999153630725 1105 20-21 0.4290801490785512 1105 22-23 0.3310913735031207 1105 24-25 0.4804902111361571 1105 26-27 0.4002934419059514 1105 28-29 0.4821139055372399 1105 30-31 0.5711409268884893 1105 32-33 0.599205817628139 1105 34-35 0.605908229520324 1105 36-37 0.5568530664606968 1105 38-39 0.4198977470498946 1105 40-41 0.5490317889128775 1105 42-43 0.5696893873341153 1105 44-45 0.46625667884553934 1105 46-47 0.5872714282350202 1105 48-49 0.6336379652762076 1105 50-51 0.8342997115842437 1105 52-53 0.8391580426353045 1105 54-55 1.028353574519599 1105 56-57 1.0041459557512518 1105 58-59 1.0217458114069906 1105 60-61 0.961679825582948 1105 62-63 0.891462264573498 1105 64-65 1.0324187821191089 1105 66-67 0.9057852533876876 1105 68-69 1.0027548411526155 1105 70-71 1.165647677000912 1105 72-73 1.2161620112039735 1105 74-75 1.300603877603109 1105 76-77 1.1045288537484517 1105 78-79 1.525455997775019 1105 80-81 1.5629007781669166 1105 82-83 1.7006429531097247 1105 84-85 1.5923479465074166 1105 86-87 1.7662543935000805 1105 88-89 2.0777421706233135 1105 90-91 2.209910941616709 1105 92-93 2.4365186779490706 1105 94-95 2.539265762556436 1105 96-97 2.3137164287555585 1105 98-99 2.3594828585382466 1105 100-101 1.8554266240327468 1106 1 -0.014982355096648803 1106 2 0.05952932557705992 1106 3 0.09494381499126803 1106 4 0.10151873728380423 1106 5 0.052490325077449995 1106 6 -0.006378431451544486 1106 7 0.12842882344054374 1106 8 0.19300387589406398 1106 9 -0.2850554299039416 1106 10-11 0.10379850357181652 1106 12-13 0.12310956291629083 1106 14-15 0.10212402852548053 1106 16-17 0.0464094311270955 1106 18-19 0.211536289277781 1106 20-21 0.2765909539939386 1106 22-23 0.2503695103157071 1106 24-25 0.21202159619760153 1106 26-27 0.11250435114465773 1106 28-29 0.13494418368760108 1106 30-31 0.16116992064264224 1106 32-33 0.09230692995861034 1106 34-35 0.1949268451363011 1106 36-37 0.38937179723878756 1106 38-39 0.3723056512350169 1106 40-41 0.35710092682288064 1106 42-43 0.3373925129692026 1106 44-45 0.19254784929901092 1106 46-47 -0.4693988287797026 1106 48-49 -0.12181360567042532 1106 50-51 0.30043561641970484 1106 52-53 0.41676172240335063 1106 54-55 0.4418190733070091 1106 56-57 0.3454244583728858 1106 58-59 0.29307959184027155 1106 60-61 0.44646691116871295 1106 62-63 0.30910848657894263 1106 64-65 0.5630935643272394 1106 66-67 0.3985838958296668 1106 68-69 0.19476225217294996 1106 70-71 0.36144155037678516 1106 72-73 0.887669889646979 1106 74-75 0.841658491539075 1106 76-77 0.7591763222143548 1106 78-79 1.1777036911457444 1106 80-81 0.714529983277405 1106 82-83 1.1880238958131848 1106 84-85 1.4180538224846018 1106 86-87 1.856514050181513 1106 88-89 1.8602331270107477 1106 90-91 1.886464613933093 1106 92-93 1.9328006354339315 1106 94-95 2.0776482768839593 1106 96-97 2.0194806309531224 1106 98-99 1.975997104768382 1106 100-101 1.593424700148125 1107 1 0.14059191082648326 1107 2 0.17268678145516603 1107 3 0.19580001768149202 1107 4 0.10238673564054324 1107 5 0.15484864488644234 1107 6 0.1001075581438684 1107 7 0.19024950490467063 1107 8 0.16008135715318872 1107 9 0.14963725230860092 1107 10-11 0.21953412607794576 1107 12-13 0.21815741671510125 1107 14-15 0.3389714536128281 1107 16-17 0.4636064960398656 1107 18-19 0.3224448403225111 1107 20-21 0.30025590825523096 1107 22-23 0.3172796151344386 1107 24-25 0.31619339804667135 1107 26-27 0.37324186502490164 1107 28-29 0.29086560827872177 1107 30-31 0.2112634724805318 1107 32-33 0.35868419604850743 1107 34-35 0.3759087733617008 1107 36-37 0.5720817906088129 1107 38-39 0.6233139773485945 1107 40-41 0.5531278209425423 1107 42-43 0.564780830378389 1107 44-45 0.54019074973138 1107 46-47 0.6580563985309169 1107 48-49 0.9145337238313545 1107 50-51 1.0636854038943326 1107 52-53 0.9613483249666785 1107 54-55 0.7701937524852198 1107 56-57 0.8034121583399418 1107 58-59 0.8578243783590445 1107 60-61 1.0464191183819693 1107 62-63 1.2897652204809091 1107 64-65 1.2315977111141407 1107 66-67 1.168651375177081 1107 68-69 1.3681459407998915 1107 70-71 1.3637201477500156 1107 72-73 1.3399855010082398 1107 74-75 1.4809854470775505 1107 76-77 1.4595934413871952 1107 78-79 1.7889000157787578 1107 80-81 1.8427025091363554 1107 82-83 1.7097401246165767 1107 84-85 1.8214580925158792 1107 86-87 2.003821790985981 1107 88-89 2.0156400164934887 1107 90-91 2.2494717767498997 1107 92-93 2.568620210545891 1107 94-95 2.5780845131714933 1107 96-97 2.562680022459432 1107 98-99 2.3285230043913288 1107 100-101 1.9928644059886071 1108 1 0.08170969435884956 1108 2 0.12082466660710978 1108 3 0.09934895705300661 1108 4 0.15641106513118075 1108 5 0.1011352116737001 1108 6 0.24478017003867336 1108 7 0.1521166970178811 1108 8 0.09232427219308903 1108 9 0.1594299545224871 1108 10-11 0.13472456596434768 1108 12-13 0.22227029328556824 1108 14-15 0.2959257152527428 1108 16-17 0.32119040222347195 1108 18-19 0.3070247032628757 1108 20-21 0.29244291501907327 1108 22-23 0.2825729001462136 1108 24-25 0.3140399373095306 1108 26-27 0.24888361913777857 1108 28-29 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-1.785142430152547 2316 78-79 -2.1915061195193175 2316 80-81 -2.3357981698630113 2316 82-83 -2.2453828649983016 2316 84-85 -2.149379163160628 2316 86-87 -2.54311367164744 2316 88-89 -2.9840217940011655 2316 90-91 -2.907741469666277 2316 92-93 -3.173756686836441 2316 94-95 -3.095174474153195 2316 96-97 -3.169599666321062 2316 98-99 -2.9587083028183816 2316 100-101 -2.5283640686178632 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 13.0 3 40.0 4 76.0 5 158.0 6 325.0 7 797.0 8 1835.0 9 3162.0 10 5054.0 11 7646.0 12 11199.0 13 15469.0 14 20343.0 15 26365.0 16 32782.0 17 39064.0 18 39329.0 19 38078.0 20 40382.0 21 42841.0 22 47311.0 23 52276.0 24 58363.0 25 66607.0 26 76801.0 27 88994.0 28 102677.0 29 118010.0 30 134265.0 31 152689.0 32 174274.0 33 199283.0 34 224738.0 35 246853.0 36 240215.0 37 157530.0 38 33070.0 39 397.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 40.672570705627884 20.683581762297212 15.970793492273454 22.67305403980145 2 17.052926519298317 21.468293122254355 38.125386516696516 23.35339384175081 3 20.22293344045619 24.22847736836272 30.06316540838655 25.485423782794538 4 14.627837283090134 17.778746934153826 35.54513045600586 32.04828532675018 5 14.2164728881531 37.02887444617318 34.17137020028427 14.583282465389452 6 33.01880639808843 36.33752369154835 16.51532483578461 14.128345074578613 7 28.922931159827648 32.12869466825057 20.34028578276173 18.608088389160052 8 26.292926330496684 36.07890334576209 18.96402648569946 18.664143838041763 9 25.891855795457232 16.90721962972995 19.79829641049073 37.40262816432209 10-11 25.344204862860202 26.68187352434331 26.96957281426761 21.004348798528873 12-13 25.882993352968075 23.633493390778497 27.594685095212242 22.888828161041182 14-15 22.625755658259415 25.36260993529817 25.64970905981688 26.36192534662553 16-17 22.470252801672142 28.539025355387942 26.378870016576567 22.611851826363345 18-19 22.41535767257456 27.044713523046955 28.655457444071587 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22.691337588002895 26.598527131271144 28.355233897788295 22.354901382937665 46-47 22.7910011999307 26.824672879845686 27.778915869213556 22.605410051010058 48-49 22.788640549335394 26.76995780036978 27.884885074326483 22.556516575968335 50-51 22.941481968136653 27.01174489796735 27.610493026441823 22.436280107454174 52-53 23.055974226496822 26.869785312832217 27.502839782644095 22.571400678026865 54-55 22.882351811440245 26.916770496486592 27.61735170340922 22.583525988663943 56-57 22.85483879357151 27.06727974229698 27.544931383089178 22.532950081042337 58-59 22.92899254364481 26.81340463132822 27.572106380322136 22.685496444704835 60-61 22.883666738553146 26.944085790043736 27.65510174604121 22.51714572536191 62-63 22.67907560765584 26.98344451949332 27.759020003905388 22.57845986894545 64-65 22.55540636066441 26.872427563612746 27.692625977478308 22.879540098244536 66-67 22.597601728090616 26.920349099272578 27.550657227016362 22.931391945620444 68-69 22.665588613453096 26.78954232359284 27.657067030979825 22.887802031974243 70-71 22.73985258307706 26.782334776133855 27.537964538956018 22.939848101833068 72-73 22.76776390283718 26.80965946601262 27.399282201887722 23.023294429262474 74-75 22.68359157633647 27.0954960528709 27.060299742900863 23.160612627891766 76-77 22.6189137118787 26.92279209999932 27.430582380671986 23.027711807449993 78-79 22.7199505973621 26.93466317038147 27.422486204561498 22.922900027694933 80-81 22.70110762245068 27.053137583264782 27.282654131768112 22.963100662516425 82-83 22.76516477286485 26.97379335297276 27.278670730631084 22.982371143531307 84-85 22.779347555102387 26.891952944829782 27.26661195011163 23.0620875499562 86-87 22.748804261403084 26.839724811060695 27.210820929116803 23.200649998419422 88-89 22.611258771891187 27.033681339405625 27.0472550977671 23.307804790936093 90-91 22.727419164742862 26.85187871724924 27.198173318987433 23.222528799020466 92-93 22.619815850319107 26.652839933047396 27.11284395500609 23.614500261627413 94-95 22.541549140202534 26.737203889338897 27.08802238578989 23.63322458466868 96-97 22.561829673640503 26.64735207517121 27.042035752873822 23.74878249831446 98-99 22.523180311056297 26.64667974043015 26.835240557648454 23.9948993908651 100-101 22.88535463993391 26.437286177526946 27.032082549605214 23.64527663293393 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 447.0 1 321.0 2 264.0 3 363.0 4 334.5 5 320.5 6 623.0 7 1476.5 8 2262.5 9 2479.5 10 2175.5 11 1783.5 12 1745.5 13 1885.0 14 2142.0 15 2344.5 16 2539.0 17 2789.0 18 3057.5 19 3406.5 20 3891.5 21 4584.0 22 5444.0 23 6601.5 24 8247.5 25 10491.5 26 13547.0 27 17084.5 28 20302.5 29 23621.0 30 27461.5 31 32247.0 32 37601.0 33 41250.5 34 44484.0 35 48550.5 36 54206.0 37 59640.0 38 62806.5 39 65718.5 40 69637.5 41 75202.0 42 79808.5 43 84020.0 44 88384.0 45 91919.5 46 94108.0 47 95974.5 48 96171.0 49 95045.0 50 94844.5 51 92505.5 52 89591.5 53 88077.5 54 87274.5 55 82486.0 56 73986.5 57 66761.5 58 60279.5 59 52822.5 60 43783.5 61 35965.0 62 30094.0 63 24058.5 64 19184.5 65 15731.5 66 12315.0 67 9615.5 68 7305.0 69 5235.0 70 4011.0 71 3078.5 72 2402.5 73 1953.0 74 1570.0 75 1263.0 76 928.0 77 596.5 78 366.5 79 239.0 80 156.5 81 97.5 82 64.0 83 31.5 84 19.5 85 11.0 86 5.5 87 4.5 88 3.0 89 1.5 90 2.0 91 1.5 92 0.5 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 2.000551351952598E-4 2 0.041451424012457834 3 0.0 4 4.001102703905197E-5 5 0.04049115936352059 6 8.002205407810393E-5 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.005421494163791541 26-27 0.0017404796761987603 28-29 1.000275675976299E-4 30-31 0.0012403418382106108 32-33 3.8010475687099365E-4 34-35 0.009882723678645833 36-37 0.019825463897850247 38-39 0.004001102703905196 40-41 0.0010202811894958251 42-43 0.0013603749193277668 44-45 0.006981924218314568 46-47 0.0 48-49 0.0 50-51 5.201433515076755E-4 52-53 0.0 54-55 0.001160319784132507 56-57 0.0 58-59 0.020125546600643137 60-61 0.0 62-63 0.00796219438077134 64-65 0.013703776760875296 66-67 0.005461505190830593 68-69 0.01448399178813681 70-71 2.000551351952598E-4 72-73 4.001102703905197E-5 74-75 0.0187851771948349 76-77 0.04537250466228493 78-79 0.026367266818735247 80-81 0.023226401196169665 82-83 0.030628441198394276 84-85 0.014884102058527329 86-87 0.009122514164903847 88-89 0.07382034488705087 90-91 0.022866301952818194 92-93 0.021145827790138964 94-95 0.023326428763767292 96-97 0.0341894226048699 98-99 0.04713298985200321 100-101 0.012183357733391324 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2499311.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.37143265071932 #Duplication Level Percentage of deduplicated Percentage of total 1 76.95995951608217 43.383431746629114 2 13.256906093583906 14.946215780227515 3 4.211246554073813 7.1218200449563716 4 1.8387656195780249 4.1461540913800325 5 1.029575471340756 2.9019322170759017 6 0.5956669893749499 2.0147160944284037 7 0.3893902801204855 1.5365341565459674 8 0.27377337103136623 1.2346397717324045 9 0.20034583694282987 1.0164403668667257 >10 1.1049761295495513 11.548290683345723 >50 0.09275173707845773 3.6026058619273065 >100 0.04330635801147579 4.441094814026916 >500 0.0022713485837267395 0.7823193513907929 >1k 9.227353621324234E-4 0.7694754217730305 >5k 1.419592864819113E-4 0.554329597693493 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7411 0.2965217213864141 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6443 0.25779104721261176 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4728 0.18917213584063766 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 4.001102703905196E-5 0.0 5 0.0 0.0 0.0 4.001102703905196E-5 0.0 6 0.0 0.0 0.0 4.001102703905196E-5 0.0 7 0.0 0.0 0.0 4.001102703905196E-5 0.0 8 0.0 0.0 0.0 8.002205407810392E-5 0.0 9 0.0 0.0 0.0 1.2003308111715589E-4 0.0 10-11 0.0 0.0 0.0 1.6004410815620784E-4 0.0 12-13 0.0 0.0 0.0 1.6004410815620784E-4 0.0 14-15 0.0 0.0 0.0 1.6004410815620784E-4 0.0 16-17 0.0 0.0 0.0 2.4006616223431177E-4 0.0 18-19 0.0 0.0 0.0 2.8007718927336374E-4 0.0 20-21 0.0 0.0 0.0 3.200882163124157E-4 0.0 22-23 0.0 0.0 0.0 4.001102703905196E-4 0.0 24-25 6.001654055857794E-5 0.0 0.0 5.601543785467275E-4 0.0 26-27 1.000275675976299E-4 0.0 0.0 8.202260543005652E-4 0.0 28-29 1.2003308111715589E-4 0.0 0.0 0.0019205292978744942 0.0 30-31 1.6004410815620784E-4 0.0 0.0 0.005641554812506327 0.0 32-33 1.6004410815620784E-4 0.0 0.0 0.013383688544562882 0.0 34-35 2.2006064871478578E-4 0.0 0.0 0.027187492873035807 0.0 36-37 2.8007718927336374E-4 0.0 0.0 0.05415492509735683 0.0 38-39 3.200882163124157E-4 0.0 0.0 0.09354578121730349 0.0 40-41 3.200882163124157E-4 0.0 0.0 0.1379980322576902 0.0 42-43 3.200882163124157E-4 0.0 0.0 0.1818701234060107 0.0 44-45 3.200882163124157E-4 0.0 0.0 0.2386057597473864 0.0 46-47 3.200882163124157E-4 0.0 0.0 0.308765095660364 0.0 48-49 3.200882163124157E-4 0.0 0.0 0.38448596433176985 0.0 50-51 3.200882163124157E-4 0.0 0.0 0.4648481121397057 0.0 52-53 3.600992433514677E-4 0.0 0.0 0.5480310373538947 0.0 54-55 3.600992433514677E-4 0.0 0.0 0.64107668073321 0.0 56-57 3.600992433514677E-4 0.0 0.0 0.7533876336318289 0.0 58-59 3.600992433514677E-4 0.0 0.0 0.878222037993671 0.0 60-61 3.600992433514677E-4 0.0 0.0 1.0109786257092455 0.0 62-63 3.600992433514677E-4 0.0 0.0 1.1463759412093972 0.0 64-65 3.600992433514677E-4 0.0 0.0 1.2911558425502068 0.0 66-67 3.600992433514677E-4 2.000551351952598E-5 0.0 1.4570815716811554 0.0 68-69 4.001102703905196E-4 4.001102703905196E-5 0.0 1.6449933601700628 0.0 70-71 4.001102703905196E-4 4.001102703905196E-5 0.0 1.8533107724488869 0.0 72-73 4.001102703905196E-4 8.002205407810392E-5 0.0 2.0603278263489417 0.0 74-75 4.001102703905196E-4 8.002205407810392E-5 0.0 2.2776877307385917 0.0 76-77 4.001102703905196E-4 8.002205407810392E-5 0.0 2.522035072866082 0.0 78-79 4.001102703905196E-4 8.002205407810392E-5 0.0 2.792329566028397 0.0 80-81 4.001102703905196E-4 8.002205407810392E-5 0.0 3.0801889000608567 0.0 82-83 4.001102703905196E-4 8.002205407810392E-5 0.0 3.3715291934457134 0.0 84-85 4.001102703905196E-4 8.002205407810392E-5 0.0 3.664309883803976 0.0 86-87 4.201157839100456E-4 8.002205407810392E-5 0.0 3.9747154315729416 0.0 88-89 4.401212974295716E-4 8.002205407810392E-5 0.0 4.311028119349693 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4225 0.0 55.00733 1 GTATCAA 6945 0.0 40.71769 1 ATCAACG 9175 0.0 30.650436 3 TATCAAC 9385 0.0 30.18156 2 TCAACGC 9435 0.0 29.805805 4 CAACGCA 9615 0.0 29.198414 5 AACGCAG 9945 0.0 28.217113 6 TGGTATC 1830 0.0 28.04803 2 ACGCAGA 11630 0.0 24.128906 7 CGCAGAG 11660 0.0 24.026104 8 GTGGTAT 2195 0.0 23.384476 1 GAGTACT 8300 0.0 21.309734 12-13 GCAGAGT 12880 0.0 21.271093 9 GTACATG 7390 0.0 21.223055 1 TACATGG 7235 0.0 21.020409 2 AGGACCG 1030 0.0 20.75374 5 GGACCGA 910 0.0 20.349468 6 AGAGTAC 11875 0.0 19.832521 10-11 CAGAGTA 12450 0.0 19.774672 10-11 ACATGGG 7465 0.0 19.726631 3 >>END_MODULE