FastQCFastQC Report
Thu 26 May 2016
SRR938294_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938294_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2592207
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77140.2975842592817626No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58070.22401760353243394No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42010.16206267477867317No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA37250.048.8087961
GTATCAA63200.044.3566971
ATCAACG82600.033.5987053
TATCAAC84000.033.3215942
TCAACGC84950.032.6692544
CAACGCA85900.032.3620265
AACGCAG88650.031.352686
ACGCAGA104450.026.15167
CGCAGAG104700.026.0891578
GGACCGA8650.025.8154246
GTACATG74300.024.5982041
TACATGG73600.024.0189972
TGGTATC18250.023.695632
GCAGAGT114900.023.4828289
AGGACCG9950.023.4016235
ACATGGG75250.022.860823
GTGGTAT21100.022.3312851
AGAGTAC105450.021.23931310-11
GAGTACT71150.020.76143312-13
AGTACTT77550.020.05863212-13
Produced by FastQC (version 0.11.5)