##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938294_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2592207 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.631381290151598 31.0 31.0 34.0 30.0 34.0 2 31.776684500890553 33.0 31.0 34.0 30.0 34.0 3 31.856054705507702 33.0 31.0 34.0 30.0 34.0 4 35.493770366332626 37.0 35.0 37.0 33.0 37.0 5 35.511927095328424 37.0 35.0 37.0 33.0 37.0 6 35.5513155392297 37.0 35.0 37.0 33.0 37.0 7 35.49589596818464 37.0 35.0 37.0 33.0 37.0 8 35.46735040835859 37.0 35.0 37.0 33.0 37.0 9 37.06938874866089 39.0 37.0 39.0 33.0 39.0 10-11 36.93776230061874 39.0 37.0 39.0 33.0 39.0 12-13 36.814561877195764 39.0 37.0 39.0 33.0 39.0 14-15 37.927792996469805 40.0 38.0 41.0 33.0 41.0 16-17 37.77912952167786 40.0 37.0 41.0 33.0 41.0 18-19 37.76872661018198 40.0 37.5 41.0 32.5 41.0 20-21 37.86606123662192 40.0 38.0 41.0 33.0 41.0 22-23 37.79294265465683 40.0 37.5 41.0 33.0 41.0 24-25 37.697254115894296 40.0 37.0 41.0 32.5 41.0 26-27 37.524386555549 40.0 37.0 41.0 32.0 41.0 28-29 37.356464202125835 39.5 37.0 41.0 32.0 41.0 30-31 37.160009405113094 39.0 36.5 41.0 31.0 41.0 32-33 36.912779727853525 39.0 36.0 41.0 31.0 41.0 34-35 36.67543872846574 39.0 36.0 40.0 30.0 41.0 36-37 36.41294773141188 39.0 35.0 40.0 30.0 41.0 38-39 36.177280981032766 38.5 35.0 40.0 30.0 41.0 40-41 36.13987752521307 39.0 35.0 40.0 30.0 41.0 42-43 35.98963624432771 39.0 35.0 40.0 29.0 41.0 44-45 35.72394392114519 38.0 35.0 40.0 28.5 41.0 46-47 35.38220153714576 38.0 34.5 40.0 27.0 41.0 48-49 35.040768927790104 38.0 34.0 40.0 26.0 41.0 50-51 34.32272982057374 37.5 33.5 39.5 24.5 40.5 52-53 34.40354589737625 38.0 33.5 39.5 25.5 40.5 54-55 34.54842514505979 38.0 34.0 40.0 25.5 41.0 56-57 34.264650932583706 38.0 33.5 40.0 24.0 41.0 58-59 33.94579233062792 37.5 33.0 40.0 23.0 41.0 60-61 33.50791950642831 37.0 33.0 40.0 22.0 41.0 62-63 33.00140864522008 36.0 32.0 39.0 20.5 41.0 64-65 32.5243385269772 36.0 31.0 39.0 18.5 40.0 66-67 31.99214626763989 35.0 31.0 39.0 16.5 40.0 68-69 31.429021872095863 35.0 30.5 38.0 10.5 40.0 70-71 30.815870607555645 35.0 29.5 37.5 7.5 39.5 72-73 30.26501182197255 34.0 29.0 37.0 4.5 39.0 74-75 29.641792109966527 34.0 29.0 36.0 2.0 39.0 76-77 28.907764696260756 34.0 27.5 36.0 2.0 38.5 78-79 28.140032026763294 33.0 26.0 35.0 2.0 37.0 80-81 27.48856727105513 33.0 26.0 35.0 2.0 37.0 82-83 26.83625941138188 32.0 25.0 35.0 2.0 36.0 84-85 26.17280564399371 32.0 23.5 35.0 2.0 36.0 86-87 25.501017665641672 31.5 20.5 34.0 2.0 35.0 88-89 24.724886747084625 31.0 18.5 34.0 2.0 35.0 90-91 23.901766139818307 31.0 10.5 34.0 2.0 35.0 92-93 23.050650083114505 30.0 4.0 34.0 2.0 35.0 94-95 22.088137637156294 29.5 2.0 34.0 2.0 35.0 96-97 20.839895695058303 29.0 2.0 34.0 2.0 35.0 98-99 19.319153717276436 26.5 2.0 33.5 2.0 35.0 100-101 15.643069207050209 13.0 2.0 30.0 2.0 33.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.49786908269673447 1101 2 0.49969054087367226 1101 3 0.48717045774926504 1101 4 0.36786048961098317 1101 5 0.32382430914593385 1101 6 0.3322137762214865 1101 7 0.33324108819816445 1101 8 0.43997928168843714 1101 9 0.46289648520390614 1101 10-11 0.4315824137329187 1101 12-13 0.39069343562486836 1101 14-15 0.5711562153264893 1101 16-17 0.5323519288051131 1101 18-19 0.4841525210335291 1101 20-21 0.587997771372045 1101 22-23 0.6229379660286156 1101 24-25 0.6563584502956061 1101 26-27 0.7765902186585052 1101 28-29 0.5888035699793193 1101 30-31 0.5278242267718696 1101 32-33 0.6208954633374191 1101 34-35 0.5289639514908586 1101 36-37 0.6798872505144473 1101 38-39 0.5660632717421024 1101 40-41 0.6375911785058932 1101 42-43 0.6933007467960266 1101 44-45 0.6365036430322917 1101 46-47 0.708144340093547 1101 48-49 0.7895250126347122 1101 50-51 0.6168186651235246 1101 52-53 0.7049085774677266 1101 54-55 0.7463602861750545 1101 56-57 0.617371452012236 1101 58-59 0.6304290593315685 1101 60-61 0.6068280017975525 1101 62-63 0.5407492843216986 1101 64-65 0.5807455303192199 1101 66-67 0.5675114147961224 1101 68-69 0.6469144936187128 1101 70-71 0.865112731484821 1101 72-73 0.5917406290818192 1101 74-75 0.5043339365825332 1101 76-77 0.632999635362669 1101 78-79 0.49884428013159265 1101 80-81 0.6606780220128492 1101 82-83 0.7324236141823697 1101 84-85 0.8578199274888583 1101 86-87 0.9605851145059532 1101 88-89 1.0085945306359925 1101 90-91 1.039222996492878 1101 92-93 0.9480771939171824 1101 94-95 0.8997856768092767 1101 96-97 0.9095946370947736 1101 98-99 1.1517104750211153 1101 100-101 1.0541861239267387 1102 1 0.35660993515749695 1102 2 0.4439979248272721 1102 3 0.33189701374652003 1102 4 0.349620346313408 1102 5 0.3273975926713746 1102 6 0.37425999501664364 1102 7 0.22837732743585804 1102 8 0.30896471221375066 1102 9 0.42089271631785863 1102 10-11 0.3970448347427933 1102 12-13 0.38150011707409703 1102 14-15 0.4394491296875458 1102 16-17 0.45464362460406704 1102 18-19 0.41598046406075895 1102 20-21 0.33004923397950137 1102 22-23 0.45290693597740983 1102 24-25 0.36806280582275264 1102 26-27 0.3452171555396504 1102 28-29 0.33659201294995 1102 30-31 0.23995058433661143 1102 32-33 0.28024617949235164 1102 34-35 0.3692561566887278 1102 36-37 0.3520615847999906 1102 38-39 0.3182552685192377 1102 40-41 0.35183711829693465 1102 42-43 0.5798446250197316 1102 44-45 0.4349148511400571 1102 46-47 0.4543596393737275 1102 48-49 0.31298274917226365 1102 50-51 0.5373699146490836 1102 52-53 0.5904199240787307 1102 54-55 0.40824592778461977 1102 56-57 0.36708553938912303 1102 58-59 0.5552294615236448 1102 60-61 0.5222898401616121 1102 62-63 0.32509042843830827 1102 64-65 0.3860044914117964 1102 66-67 0.29479072520983607 1102 68-69 0.3605457420788589 1102 70-71 0.5082392588491942 1102 72-73 0.4420268746453857 1102 74-75 0.32156127498987885 1102 76-77 0.4794187774416834 1102 78-79 0.2216343045694238 1102 80-81 0.21023621001766912 1102 82-83 0.4958956338658105 1102 84-85 0.30416740413308574 1102 86-87 0.1631756410180003 1102 88-89 0.3174983574536796 1102 90-91 0.5301187395161122 1102 92-93 0.544917921858211 1102 94-95 0.46243758089579146 1102 96-97 0.7156643082161871 1102 98-99 0.894841497081746 1102 100-101 0.8785150824223802 1103 1 0.5244950191641671 1103 2 0.48754672564810164 1103 3 0.4668110832604384 1103 4 0.4222508452232461 1103 5 0.39458689624967036 1103 6 0.49716944737011204 1103 7 0.5390929873050396 1103 8 0.4496694704540971 1103 9 0.46335776697836906 1103 10-11 0.43551475907266024 1103 12-13 0.4674306031584905 1103 14-15 0.5015820013048327 1103 16-17 0.5712141004281079 1103 18-19 0.5552070896860002 1103 20-21 0.4892675230650454 1103 22-23 0.6349705501880081 1103 24-25 0.6497528789277922 1103 26-27 0.633594095388311 1103 28-29 0.6368324900565341 1103 30-31 0.6633666417946102 1103 32-33 0.6893618650124225 1103 34-35 0.6761995323460255 1103 36-37 0.7189542999468443 1103 38-39 0.5764924110099443 1103 40-41 0.7871119034707235 1103 42-43 0.7446179352672573 1103 44-45 0.7517995503833603 1103 46-47 0.5931085171472361 1103 48-49 0.5661955233161677 1103 50-51 0.6815567813927075 1103 52-53 0.6131257724819648 1103 54-55 0.7463230728404113 1103 56-57 0.7900895794045226 1103 58-59 0.5965722856867828 1103 60-61 0.7335169146774518 1103 62-63 0.6746367282972301 1103 64-65 0.5665611157544959 1103 66-67 0.643638038278965 1103 68-69 0.6892154227252583 1103 70-71 0.6160066693917123 1103 72-73 0.8185963885609979 1103 74-75 0.5023391382706279 1103 76-77 0.4537246001940254 1103 78-79 0.3795411918337557 1103 80-81 0.4248620276398327 1103 82-83 0.6510674948379815 1103 84-85 0.3759722649770012 1103 86-87 0.5801597374653511 1103 88-89 0.5525438877858591 1103 90-91 0.5467212789543581 1103 92-93 0.6697255401729514 1103 94-95 0.6656070691605045 1103 96-97 0.7723175081648854 1103 98-99 0.9608011610550697 1103 100-101 0.6449011924647063 1104 1 0.3123961624192688 1104 2 0.27374378846359093 1104 3 0.35960245064233476 1104 4 0.18774706063711477 1104 5 0.22618679254845375 1104 6 0.19020164049837973 1104 7 0.2351509430693568 1104 8 0.13879761770982668 1104 9 0.17325513327242703 1104 10-11 0.1984054813719851 1104 12-13 0.21065634647763432 1104 14-15 0.32271125165686243 1104 16-17 0.28322056650408456 1104 18-19 0.28904329767810566 1104 20-21 0.3983476703817814 1104 22-23 0.37668284030741006 1104 24-25 0.3938803526339427 1104 26-27 0.39166963715093317 1104 28-29 0.3658307057855694 1104 30-31 0.2921423319922951 1104 32-33 0.27126644718293136 1104 34-35 0.3693018762985716 1104 36-37 0.32877458929357317 1104 38-39 0.38318933657381393 1104 40-41 0.3311376457731896 1104 42-43 0.5179740294930326 1104 44-45 0.38881732700890126 1104 46-47 0.38000429233925814 1104 48-49 0.34587726478496705 1104 50-51 0.20946486347934012 1104 52-53 0.3636527932577849 1104 54-55 0.45195439213340904 1104 56-57 0.4523536464239726 1104 58-59 0.35558285406096246 1104 60-61 0.40377626939153544 1104 62-63 0.5353269132818994 1104 64-65 0.4053160148745576 1104 66-67 0.37857918529577717 1104 68-69 0.3136786799602014 1104 70-71 0.40809449517366403 1104 72-73 0.4948345760263635 1104 74-75 0.2586492811648071 1104 76-77 0.35811766847924176 1104 78-79 0.5130262009076141 1104 80-81 0.4847199340952315 1104 82-83 0.3434793233852993 1104 84-85 0.3583130022723324 1104 86-87 0.3618323898273488 1104 88-89 0.3663618209799111 1104 90-91 0.42684648292550165 1104 92-93 0.32670643678181577 1104 94-95 0.1735655657978441 1104 96-97 0.15055143574686625 1104 98-99 0.23866285717999958 1104 100-101 0.11994255857575631 1105 1 0.38149963857075164 1105 2 0.38297808177389214 1105 3 0.31587895300332036 1105 4 0.33322955026652323 1105 5 0.21046293371254876 1105 6 0.21545163727341077 1105 7 0.19700879735098198 1105 8 0.2731675287804549 1105 9 0.25594894949569635 1105 10-11 0.3516369956776515 1105 12-13 0.32639973943098255 1105 14-15 0.45251540062968587 1105 16-17 0.4773817696582867 1105 18-19 0.41631587417291627 1105 20-21 0.35877277700549826 1105 22-23 0.3892384250071572 1105 24-25 0.3526289036999941 1105 26-27 0.49656428498703775 1105 28-29 0.42876883361854823 1105 30-31 0.4053683423453833 1105 32-33 0.3645411208310989 1105 34-35 0.42215247531412814 1105 36-37 0.5552556654637328 1105 38-39 0.48942452857637875 1105 40-41 0.45816193012114326 1105 42-43 0.6055468294016322 1105 44-45 0.4898918877523286 1105 46-47 0.33252956844295056 1105 48-49 0.34378410424461947 1105 50-51 0.3115283542752678 1105 52-53 0.3863922259452437 1105 54-55 0.34003071770449367 1105 56-57 0.4429519616984834 1105 58-59 0.3831861591353487 1105 60-61 0.4650189832578775 1105 62-63 0.5853606679911323 1105 64-65 0.6640845300543461 1105 66-67 0.6369065857040255 1105 68-69 0.4285612817572151 1105 70-71 0.45172394647996583 1105 72-73 0.5240609942616459 1105 74-75 0.5540657049347573 1105 76-77 0.6111651443702719 1105 78-79 0.8956542276976123 1105 80-81 0.7259251891413925 1105 82-83 0.5505893735658667 1105 84-85 0.590335256423483 1105 86-87 0.7000501534657566 1105 88-89 0.5226484589678684 1105 90-91 0.47351256807337094 1105 92-93 0.6424665995907013 1105 94-95 0.6613096885163294 1105 96-97 0.6719317115710624 1105 98-99 0.7783314283207368 1105 100-101 0.5383061646721199 1106 1 0.24013232334444723 1106 2 0.19022641903839954 1106 3 0.04032306281978748 1106 4 0.2541977330510079 1106 5 0.2962738779033529 1106 6 0.0017654513912219727 1106 7 0.07333316490410624 1106 8 0.17104418771974395 1106 9 0.12913120251493382 1106 10-11 0.008872805482980084 1106 12-13 0.009822431088451822 1106 14-15 0.263955818009201 1106 16-17 0.05580821955351922 1106 18-19 -0.19548037366325133 1106 20-21 0.03399295659105661 1106 22-23 0.04632390530235142 1106 24-25 0.11485665290785363 1106 26-27 0.03768117756976608 1106 28-29 0.054357307849947745 1106 30-31 0.010747683932493146 1106 32-33 0.19324684448800156 1106 34-35 0.11923484272552543 1106 36-37 0.0827857693277565 1106 38-39 0.10993046077118862 1106 40-41 0.276873778313643 1106 42-43 -0.18936778141197408 1106 44-45 0.09924604028869766 1106 46-47 0.003951903257650713 1106 48-49 0.25253421813061294 1106 50-51 0.0406629219640422 1106 52-53 0.22867720919134626 1106 54-55 0.08991442745212197 1106 56-57 -0.30253524257755515 1106 58-59 -0.6963515016969808 1106 60-61 0.006478609408119951 1106 62-63 0.0113702040449013 1106 64-65 0.19021655682008998 1106 66-67 -0.3934179640666855 1106 68-69 -0.5672963683445218 1106 70-71 -0.47233839218154827 1106 72-73 -0.1811172120086617 1106 74-75 -0.15883201025915383 1106 76-77 -0.18206433337387296 1106 78-79 -0.04499857946556318 1106 80-81 0.12239124352321795 1106 82-83 0.09096550103719281 1106 84-85 -0.11040569849791027 1106 86-87 0.03950829656730548 1106 88-89 -0.33802430355199675 1106 90-91 -0.024086398854134217 1106 92-93 -0.09192678034309054 1106 94-95 -0.4673110635361475 1106 96-97 -0.7054255386643042 1106 98-99 -0.5129233928529437 1106 100-101 -0.8149411045948565 1107 1 0.3544996658876016 1107 2 0.3048852834056177 1107 3 0.27951233661988795 1107 4 0.2867059227105031 1107 5 0.20900178585858953 1107 6 0.2686260067853823 1107 7 0.28485128233430146 1107 8 0.2323233774693918 1107 9 0.295706497292592 1107 10-11 0.2719048829015591 1107 12-13 0.26786701839974114 1107 14-15 0.30651443543438717 1107 16-17 0.36885557431853755 1107 18-19 0.36177717985754754 1107 20-21 0.4476080776577689 1107 22-23 0.4246698946232428 1107 24-25 0.3048614489928454 1107 26-27 0.40207894958167145 1107 28-29 0.47487785212420164 1107 30-31 0.4611933566867208 1107 32-33 0.591212077254653 1107 34-35 0.6051950865619915 1107 36-37 0.48800492411482566 1107 38-39 0.5058279923527138 1107 40-41 0.49190430796171114 1107 42-43 0.48215875108118667 1107 44-45 0.5842512621358011 1107 46-47 0.5027623990451033 1107 48-49 0.3415286431513991 1107 50-51 0.33687414004440797 1107 52-53 0.2649800663055828 1107 54-55 0.21543523469708958 1107 56-57 0.23392890324346638 1107 58-59 0.2898508630358876 1107 60-61 0.26580661904537095 1107 62-63 0.30055885172425434 1107 64-65 0.3500005702040454 1107 66-67 0.3633140863034754 1107 68-69 0.3507370990621368 1107 70-71 0.3841872887873876 1107 72-73 0.4719583557576392 1107 74-75 0.5584822322349119 1107 76-77 0.5465334817151337 1107 78-79 0.46482018362036115 1107 80-81 0.46711110457449934 1107 82-83 0.6228703607360551 1107 84-85 0.6852974225888353 1107 86-87 0.4650211520786698 1107 88-89 0.5476221285614855 1107 90-91 0.6376677034890577 1107 92-93 0.6999160558336648 1107 94-95 0.6768863623561963 1107 96-97 0.7005846570860932 1107 98-99 0.6817848849643475 1107 100-101 0.25335289165843555 1108 1 0.24892631617388972 1108 2 0.2692565036704835 1108 3 0.1966114257052496 1108 4 0.28431285219444646 1108 5 0.28317003084271164 1108 6 0.2345387374086414 1108 7 0.20669373334552432 1108 8 0.1560811380380258 1108 9 0.16467251045051512 1108 10-11 0.20593608849233647 1108 12-13 0.2504214916804699 1108 14-15 0.3612453658251056 1108 16-17 0.337298305161184 1108 18-19 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78-79 -0.2967504996492707 2316 80-81 -0.14184434520114308 2316 82-83 -0.06181653831667333 2316 84-85 -0.25605138501307323 2316 86-87 -0.12008470367024415 2316 88-89 -0.31521469867433183 2316 90-91 -0.43539791401764916 2316 92-93 -0.526360920021613 2316 94-95 -0.5700000630610553 2316 96-97 -0.5055866262405146 2316 98-99 -0.5750349020777428 2316 100-101 -0.503983932609744 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6317.0 3 1790.0 4 1833.0 5 2030.0 6 2351.0 7 2820.0 8 3548.0 9 4592.0 10 6136.0 11 7609.0 12 9989.0 13 12423.0 14 14799.0 15 17935.0 16 21244.0 17 25153.0 18 24097.0 19 22179.0 20 23420.0 21 26025.0 22 28961.0 23 32769.0 24 37427.0 25 42885.0 26 49458.0 27 57632.0 28 67513.0 29 80889.0 30 96240.0 31 116935.0 32 144613.0 33 179818.0 34 227535.0 35 287423.0 36 352732.0 37 362653.0 38 179882.0 39 10551.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.814406889687106 18.49721636609809 14.492438902249782 24.19593784196502 2 15.671599165455477 19.376260558200066 40.235049480648094 24.717090795696368 3 19.431496545554605 23.108082344777657 29.147823710979743 28.312597398688 4 13.143987214138859 15.996027768568503 36.20307627942556 34.65690873786708 5 14.369956033615313 36.51965788741348 34.05466869277683 15.055717386194377 6 33.32783930149883 35.17039861967275 17.0503997004659 14.45136237836252 7 29.366037686337208 31.63050579681454 20.33185957673587 18.67159694011238 8 26.328306855438722 35.15087151686538 19.36500904549917 19.15581258219673 9 26.159126711839757 16.875770390603957 19.819135248365825 37.14596764919046 10-11 25.21120547762289 26.10316776377867 27.238492480502252 21.447134278096186 12-13 25.85762736457863 23.258016321205083 27.602066382728456 23.28228993148783 14-15 22.78175473042551 24.93191394037832 25.652839914648105 26.633491414548065 16-17 22.664515217840947 28.057112824106518 26.34373581865162 22.93463613940092 18-19 22.58990584668997 26.796057119095877 28.361767137706572 22.252269896507578 20-21 23.01673420466648 26.001515624888437 28.650038276572015 22.331711893873067 22-23 22.69834494491126 25.875190846176054 28.472339075571224 22.954125133341464 24-25 22.529110107909574 26.128013062636192 28.59559290433279 22.747283925121447 26-27 22.33383018830057 26.463239165036644 28.420348565244442 22.782582081418347 28-29 22.340929703027612 26.665515311734172 28.438820600891496 22.55473438434672 30-31 22.4962687330443 26.312427560158596 28.43547696726636 22.755826739530743 32-33 22.2882212865154 26.281112144591145 28.478848180067384 22.951818388826066 34-35 22.796947102261576 26.461354514478998 28.224039977669253 22.517658405590176 36-37 22.84306177070489 26.183515590778487 28.22740403262688 22.74601860588974 38-39 22.42076825023089 26.437413626882794 28.211189229162553 22.93062889372376 40-41 22.758093259554414 26.373442984330435 28.19089938654118 22.67756436957397 42-43 22.48579112471013 26.3272034849847 28.153019844194326 23.033985546110838 44-45 22.526159688145768 26.525548602614435 28.18545211359787 22.762839595641925 46-47 22.62284399317802 26.747476914274404 27.650534903626294 22.979144188921282 48-49 22.649429866103894 26.644090226028045 27.808124046320604 22.898355861547454 50-51 22.758141231513115 26.922231869403106 27.551609149128293 22.768017749955487 52-53 22.756765765824852 26.761575982558526 27.58226037747933 22.899397874137293 54-55 22.614724700842302 26.793248619568942 27.668402558079958 22.923624121508798 56-57 22.614153227447662 26.944827941409937 27.610296181399026 22.83072264974337 58-59 22.640629349409796 26.694416725036568 27.630855806630265 23.034098118923367 60-61 22.61845282624321 26.83917329844568 27.69390020366992 22.84847367164119 62-63 22.368395925885416 26.86789565998353 27.886752222069838 22.87695619206121 64-65 22.32080113963521 26.835708698591752 27.765857817583132 23.077632344189897 66-67 22.437462711412905 26.824269076180503 27.608783334526056 23.12948487788054 68-69 22.44431292785758 26.650723924221754 27.81930752658967 23.085655621330996 70-71 22.47115205972104 26.66969433945447 27.6551478122331 23.2040057885914 72-73 22.50254271687176 26.66156322683504 27.595153172647002 23.2407408836462 74-75 22.36894777975051 26.982266054898968 27.407407693217394 23.241378472133132 76-77 22.505638707576587 26.79638073294325 27.44198670785129 23.25599385162888 78-79 22.604743841618614 26.8044122568692 27.468758408971883 23.122085492540307 80-81 22.55262847737216 26.973009706752716 27.30797475291385 23.166387062961277 82-83 22.601377040300825 26.919085476109732 27.292279832304622 23.187257651284817 84-85 22.68156635795176 26.78865833915071 27.288004522991972 23.241770779905554 86-87 22.61651418478844 26.71249867862756 27.33003292435731 23.340954212226688 88-89 22.6474279028703 26.811206928486314 27.208022831529487 23.33334233711389 90-91 22.66560389359303 26.723012162847397 27.190184669363347 23.421199274196226 92-93 22.713007637729056 26.4304319197615 27.294236716160427 23.562323726349018 94-95 22.560698079282535 26.518650633708074 27.311796256425207 23.608855030584188 96-97 22.67771846132592 26.41326570383125 27.214423699550892 23.694592135291934 98-99 22.624757470824637 26.394042362566477 27.058452208078986 23.9227479585299 100-101 23.204691091776457 25.950333842103962 27.09591742467311 23.749057641446473 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 456.0 1 331.5 2 312.5 3 421.0 4 351.0 5 325.5 6 644.0 7 1458.0 8 2319.0 9 2624.5 10 2314.0 11 1973.5 12 1991.0 13 2090.5 14 2211.0 15 2396.0 16 2576.5 17 2744.5 18 2956.5 19 3215.5 20 3655.0 21 4312.0 22 5051.0 23 6310.5 24 7907.5 25 10097.0 26 13309.5 27 16761.0 28 19991.0 29 23200.0 30 27084.0 31 31859.5 32 37288.0 33 41218.0 34 44333.0 35 48774.0 36 54529.5 37 60419.5 38 64380.0 39 67444.5 40 71282.0 41 76960.0 42 82043.5 43 86270.0 44 90719.0 45 94983.0 46 97787.5 47 99481.5 48 99612.5 49 99288.5 50 99453.0 51 97062.0 52 94628.0 53 93557.0 54 92734.5 55 87154.0 56 78013.0 57 70499.0 58 64596.0 59 57349.5 60 47331.0 61 38803.0 62 32348.5 63 25674.5 64 20572.0 65 17153.5 66 13416.0 67 10309.0 68 7743.0 69 5634.0 70 4379.0 71 3345.5 72 2582.5 73 2099.5 74 1697.5 75 1379.0 76 1008.0 77 645.0 78 407.5 79 270.5 80 182.0 81 119.0 82 69.0 83 43.0 84 30.0 85 18.5 86 13.5 87 11.5 88 12.0 89 8.5 90 4.5 91 3.0 92 2.5 93 2.0 94 1.5 95 3.0 96 2.0 97 0.0 98 0.0 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.16291137243283427 2 0.04216484254536771 3 0.040621755901438426 4 0.04706414263984319 5 0.05261925455798862 6 0.058521560971018134 7 0.033562134505461944 8 0.033292094342774324 9 0.03815281727115157 10-11 0.038962937759214444 12-13 0.03531739556293151 14-15 0.03153683328530476 16-17 0.0298973037261299 18-19 0.03682190504076256 20-21 0.04615757923653473 22-23 0.04301354019952882 24-25 0.062186391750350185 26-27 0.04795141746010253 28-29 0.040776064565831355 30-31 0.04876153794816541 32-33 0.045405324497619214 34-35 0.04476880125699838 36-37 0.04212626537926948 38-39 0.023782822899560102 40-41 0.02277981658100607 42-43 0.019944394872786007 44-45 0.0037998508606758644 46-47 0.011245243917634665 48-49 0.010492989178719138 50-51 0.007734721802695541 52-53 0.008641285206003995 54-55 0.010145794683835049 56-57 0.007618990304400844 58-59 0.010049351768589468 60-61 0.01033868051432621 62-63 0.003298347701398847 64-65 0.005767286331685703 66-67 0.0036069650301847034 68-69 0.008602708039905763 70-71 0.02150677009976441 72-73 0.018941388554231973 74-75 0.018613482642397 76-77 0.011611726995567869 78-79 0.0042241996877564175 80-81 0.005612977667292774 82-83 0.004860722928377247 84-85 0.004532817016542274 86-87 0.007908319050137586 88-89 0.02711974776705718 90-91 0.03367786600375665 92-93 0.020580918113406836 94-95 0.029067894655017905 96-97 0.032134779359827365 98-99 0.019616488960951035 100-101 0.11414983448466887 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2592207.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.88403566812023 #Duplication Level Percentage of deduplicated Percentage of total 1 76.76197410492667 42.89768898835043 2 13.196394889645221 14.749356054070661 3 4.335475348216076 7.268515769938894 4 1.9262495772143124 4.305864003149846 5 1.0012022663332474 2.7975611581385 6 0.6203333964745557 2.080004019282614 7 0.3902277259801407 1.526525011015454 8 0.29102248098940187 1.301080856626926 9 0.207438837688917 1.0433267463924756 >10 1.1270383826673671 11.604132828276239 >50 0.09005123152619049 3.474935076956214 >100 0.049407242341357405 4.9102311852595415 >500 0.0022153154757883044 0.838518083971858 >1k 8.307433034117002E-4 0.6791632815357425 >5k 1.3845721723528337E-4 0.523096937034735 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7714 0.2975842592817626 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5807 0.22401760353243394 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4201 0.16206267477867317 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 3.8577166098232126E-5 0.0 5 0.0 0.0 0.0 3.8577166098232126E-5 0.0 6 0.0 0.0 0.0 3.8577166098232126E-5 0.0 7 0.0 0.0 0.0 3.8577166098232126E-5 0.0 8 0.0 0.0 0.0 3.8577166098232126E-5 0.0 9 0.0 0.0 0.0 3.8577166098232126E-5 0.0 10-11 7.715433219646425E-5 0.0 0.0 7.715433219646425E-5 0.0 12-13 7.715433219646425E-5 0.0 0.0 7.715433219646425E-5 0.0 14-15 7.715433219646425E-5 0.0 0.0 1.543086643929285E-4 0.0 16-17 9.644291524558032E-5 0.0 0.0 1.7359724744204455E-4 0.0 18-19 1.1573149829469638E-4 0.0 0.0 1.9288583049116063E-4 0.0 20-21 1.543086643929285E-4 0.0 0.0 1.9288583049116063E-4 0.0 22-23 1.543086643929285E-4 0.0 0.0 2.3146299658939276E-4 0.0 24-25 1.7359724744204455E-4 0.0 0.0 3.471944948840891E-4 0.0 26-27 2.121744135402767E-4 0.0 0.0 6.558118236699461E-4 0.0 28-29 2.3146299658939276E-4 0.0 0.0 0.0015623752269784009 0.0 30-31 2.507515796385088E-4 0.0 0.0 0.006095192243520675 0.0 32-33 2.7004016268762486E-4 0.0 0.0 0.01577806093417694 0.0 34-35 2.7004016268762486E-4 0.0 0.0 0.032404819522514984 0.0 36-37 2.7004016268762486E-4 0.0 0.0 0.0591966613777372 0.0 38-39 2.7004016268762486E-4 0.0 0.0 0.1015929669196943 0.0 40-41 2.7004016268762486E-4 0.0 0.0 0.14570595635302275 0.0 42-43 2.7004016268762486E-4 0.0 0.0 0.19506544037571072 0.0 44-45 2.7004016268762486E-4 0.0 0.0 0.2583127041937623 0.0 46-47 2.7004016268762486E-4 0.0 0.0 0.33452189582081987 0.0 48-49 2.7004016268762486E-4 0.0 0.0 0.4166333938609069 0.0 50-51 2.7004016268762486E-4 0.0 0.0 0.5065953452019842 0.0 52-53 2.7004016268762486E-4 0.0 0.0 0.597965363105647 0.0 54-55 2.7004016268762486E-4 0.0 0.0 0.6990761154491134 0.0 56-57 2.7004016268762486E-4 0.0 0.0 0.8196683366721871 0.0 58-59 2.7004016268762486E-4 0.0 0.0 0.9507728356570289 0.0 60-61 3.08617328785857E-4 0.0 0.0 1.09520574552881 0.0 62-63 3.08617328785857E-4 0.0 0.0 1.2393493266548543 0.0 64-65 3.08617328785857E-4 0.0 0.0 1.39765072773895 0.0 66-67 3.08617328785857E-4 0.0 0.0 1.5788669654853953 0.0 68-69 3.471944948840891E-4 0.0 0.0 1.7894597152156444 0.0 70-71 3.471944948840891E-4 0.0 0.0 2.0073628379215087 0.0 72-73 3.471944948840891E-4 0.0 0.0 2.2295480260642764 0.0 74-75 3.471944948840891E-4 0.0 0.0 2.4641936388567736 0.0 76-77 3.471944948840891E-4 0.0 0.0 2.721850531226866 0.0 78-79 3.471944948840891E-4 0.0 0.0 3.007533734767324 0.0 80-81 3.471944948840891E-4 0.0 0.0 3.3118497095332278 0.0 82-83 3.471944948840891E-4 0.0 0.0 3.6338340263721225 0.0 84-85 3.471944948840891E-4 0.0 0.0 3.959348153909005 0.0 86-87 3.471944948840891E-4 0.0 0.0 4.302009831776552 0.0 88-89 3.471944948840891E-4 0.0 0.0 4.672331337736531 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3725 0.0 48.808796 1 GTATCAA 6320 0.0 44.356697 1 ATCAACG 8260 0.0 33.598705 3 TATCAAC 8400 0.0 33.321594 2 TCAACGC 8495 0.0 32.669254 4 CAACGCA 8590 0.0 32.362026 5 AACGCAG 8865 0.0 31.35268 6 ACGCAGA 10445 0.0 26.1516 7 CGCAGAG 10470 0.0 26.089157 8 GGACCGA 865 0.0 25.815424 6 GTACATG 7430 0.0 24.598204 1 TACATGG 7360 0.0 24.018997 2 TGGTATC 1825 0.0 23.69563 2 GCAGAGT 11490 0.0 23.482828 9 AGGACCG 995 0.0 23.401623 5 ACATGGG 7525 0.0 22.86082 3 GTGGTAT 2110 0.0 22.331285 1 AGAGTAC 10545 0.0 21.239313 10-11 GAGTACT 7115 0.0 20.761433 12-13 AGTACTT 7755 0.0 20.058632 12-13 >>END_MODULE