FastQCFastQC Report
Thu 26 May 2016
SRR938294_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938294_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2592207
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76750.29607974980393154No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65630.2531819411026974No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47580.18355015629538846No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA42150.053.8162771
GTATCAA69650.041.239051
ATCAACG93100.030.43083
TATCAAC94950.030.0998252
TCAACGC95600.029.585294
CAACGCA97750.028.9831945
TGGTATC18600.028.378832
AACGCAG102700.027.7483526
GCACCGT5950.025.544036
ACGCAGA118950.023.8378077
GTGGTAT23700.023.6769921
CGCAGAG120100.023.6490978
TGCACCG6200.021.467585
GTACATG77000.021.2451881
GCAGAGT131850.021.0732789
GAGTACT82700.021.01996412-13
TACATGG74800.020.8524322
AGTACTT87700.019.8757212-13
AGAGTAC121650.019.71676410-11
CAGAGTA126100.019.60478210-11
Produced by FastQC (version 0.11.5)