##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938294_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2592207 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.41238527633017 31.0 31.0 34.0 30.0 34.0 2 31.672929669582714 31.0 31.0 34.0 30.0 34.0 3 31.727139846470596 31.0 31.0 34.0 30.0 34.0 4 35.465076670188765 37.0 35.0 37.0 33.0 37.0 5 35.31934949639439 37.0 35.0 37.0 33.0 37.0 6 35.359767179087164 37.0 35.0 37.0 33.0 37.0 7 35.28991087517316 37.0 35.0 37.0 32.0 37.0 8 35.2823863989257 37.0 35.0 37.0 32.0 37.0 9 36.7925686490315 39.0 37.0 39.0 32.0 39.0 10-11 36.6741840061384 38.5 35.0 39.0 32.0 39.0 12-13 36.53666007382898 38.0 35.0 39.0 32.0 39.0 14-15 37.45067292079683 39.0 36.0 40.5 32.0 41.0 16-17 37.3215402936571 39.0 36.0 40.0 32.0 41.0 18-19 37.438988090071504 39.0 36.0 40.5 32.0 41.0 20-21 37.501764519577335 39.0 36.0 41.0 32.0 41.0 22-23 37.36721739428988 39.0 36.0 40.5 32.0 41.0 24-25 37.23813163840696 39.0 36.0 40.5 31.0 41.0 26-27 36.99199311629048 39.0 36.0 40.0 31.0 41.0 28-29 36.829405599167046 39.0 36.0 40.0 30.5 41.0 30-31 36.54718488916973 38.5 35.0 40.0 30.0 41.0 32-33 36.28977566220599 38.0 35.0 40.0 30.0 41.0 34-35 36.088543661829476 38.0 35.0 40.0 30.0 41.0 36-37 36.06011267618674 38.0 35.0 40.0 30.0 41.0 38-39 35.901833649858986 38.0 35.0 40.0 29.0 41.0 40-41 35.67991213664649 38.0 34.0 40.0 28.5 41.0 42-43 35.41571255690614 38.0 34.0 40.0 27.0 41.0 44-45 35.09079907584541 38.0 33.5 40.0 26.5 41.0 46-47 34.64526559800201 38.0 33.0 40.0 25.5 41.0 48-49 34.2133625902561 37.5 33.0 40.0 24.0 41.0 50-51 33.745460528422306 37.0 32.5 40.0 23.5 41.0 52-53 33.13230482750799 37.0 31.0 39.0 22.0 40.0 54-55 32.70524036081995 36.0 31.0 39.0 19.5 40.0 56-57 32.26510595025783 36.0 31.0 39.0 18.0 40.0 58-59 31.695624809284134 35.0 30.0 38.5 15.0 40.0 60-61 31.210709059886035 35.0 29.0 38.0 13.0 40.0 62-63 30.63107980959854 34.0 28.0 38.0 8.5 40.0 64-65 30.001254722327346 34.0 28.0 38.0 7.5 39.5 66-67 29.38136537707058 34.0 26.5 37.0 6.5 39.0 68-69 28.704930393290354 33.0 26.0 36.0 2.0 39.0 70-71 27.994979567603977 32.0 25.5 36.0 2.0 38.5 72-73 27.419428888202216 32.0 25.0 35.0 2.0 38.0 74-75 26.75024467567598 31.0 23.5 35.0 2.0 37.5 76-77 25.732622819088135 30.5 22.5 34.0 2.0 36.0 78-79 26.352964867389062 31.0 24.0 34.0 2.0 36.0 80-81 25.946172894371475 31.0 22.5 34.0 2.0 36.0 82-83 25.298036576554267 31.0 20.0 34.0 2.0 36.0 84-85 24.578976910408777 30.5 18.0 34.0 2.0 35.0 86-87 23.774047172930246 30.0 13.0 34.0 2.0 35.0 88-89 22.891024327918256 29.0 6.5 34.0 2.0 35.0 90-91 22.10511641238528 29.0 2.0 34.0 2.0 35.0 92-93 21.074298464590214 28.5 2.0 33.0 2.0 34.5 94-95 19.983167432230527 26.5 2.0 33.0 2.0 34.0 96-97 18.65568220439186 25.0 2.0 32.0 2.0 34.0 98-99 17.123683602428358 21.0 2.0 32.0 2.0 34.0 100-101 13.959631310308165 2.0 2.0 28.5 2.0 33.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.41306782307137624 1101 2 0.43129205824768846 1101 3 0.4121488498924357 1101 4 0.3384089237869645 1101 5 0.20562870471032824 1101 6 0.271087265059613 1101 7 0.39079160847858674 1101 8 0.3914235580667196 1101 9 0.4133281633241168 1101 10-11 0.46152152478091324 1101 12-13 0.49371565718314514 1101 14-15 0.5804939990609554 1101 16-17 0.5758239077292302 1101 18-19 0.5860327486881545 1101 20-21 0.6218909563405148 1101 22-23 0.5498604136734997 1101 24-25 0.6016454718821578 1101 26-27 0.70900403336762 1101 28-29 0.6460147502932543 1101 30-31 0.6590968813770246 1101 32-33 0.5046488974097301 1101 34-35 0.5736560515819278 1101 36-37 0.7858854100181034 1101 38-39 0.7398255255804287 1101 40-41 0.7213107074990575 1101 42-43 0.8014818757581565 1101 44-45 0.649496659354817 1101 46-47 0.8835236126491495 1101 48-49 1.0958138785063412 1101 50-51 1.071788962314109 1101 52-53 1.0789663375703782 1101 54-55 0.9023658005546125 1101 56-57 0.9865245577195836 1101 58-59 1.122870081905205 1101 60-61 0.9878281783686198 1101 62-63 0.8360609273431372 1101 64-65 0.7995796863648614 1101 66-67 0.7896551447261331 1101 68-69 1.1235664325178512 1101 70-71 0.7889050034718572 1101 72-73 0.6514830618056813 1101 74-75 0.7561711237235578 1101 76-77 0.8082399945762546 1101 78-79 0.8955981083511304 1101 80-81 0.7593842966973092 1101 82-83 0.9142994977269154 1101 84-85 0.8252441835295912 1101 86-87 1.0029891833425317 1101 88-89 1.0932067972939592 1101 90-91 1.0872378950578643 1101 92-93 1.165007673047409 1101 94-95 1.2632398464821364 1101 96-97 1.3029656443298983 1101 98-99 1.023249511413507 1101 100-101 0.7681200293043595 1102 1 0.30658513930488596 1102 2 0.2797517091929045 1102 3 0.39683109151398455 1102 4 0.2921177140810869 1102 5 0.29280084158881436 1102 6 0.29478014079957404 1102 7 0.30170256688100494 1102 8 0.3667485079591799 1102 9 0.38067169795325384 1102 10-11 0.44517622889672737 1102 12-13 0.41394312210786666 1102 14-15 0.47900750312615514 1102 16-17 0.42844115271348215 1102 18-19 0.5010942261447582 1102 20-21 0.48641831405840463 1102 22-23 0.5525830343144733 1102 24-25 0.5630880529698103 1102 26-27 0.5571773555141561 1102 28-29 0.5351018774831644 1102 30-31 0.5695350643211512 1102 32-33 0.5943626185521182 1102 34-35 0.5456944629232154 1102 36-37 0.7003238726322181 1102 38-39 0.7746065672845504 1102 40-41 0.7194150277345202 1102 42-43 0.7131078238061335 1102 44-45 0.6854146927420857 1102 46-47 0.6316237711288153 1102 48-49 0.6625418062183073 1102 50-51 0.7444597940659747 1102 52-53 0.8987743807460902 1102 54-55 0.9919396867022883 1102 56-57 0.7814240762876707 1102 58-59 0.8278527856481261 1102 60-61 0.9984756143941063 1102 62-63 0.8061121002923919 1102 64-65 0.8376168858028556 1102 66-67 0.9289844861033103 1102 68-69 0.8222802835070162 1102 70-71 0.6469853852879801 1102 72-73 0.6189502005819243 1102 74-75 0.6169433291877731 1102 76-77 0.4560414222249598 1102 78-79 0.5434898461005844 1102 80-81 0.7157924017999555 1102 82-83 0.6514683884358803 1102 84-85 0.5973788978999686 1102 86-87 0.37415287685085374 1102 88-89 0.5883575194418285 1102 90-91 0.7945620026109559 1102 92-93 0.7113538179297798 1102 94-95 0.6972972420886521 1102 96-97 0.7602281578972203 1102 98-99 0.6262017777392437 1102 100-101 0.4932094238188327 1103 1 0.28527886766194754 1103 2 0.31665372278787984 1103 3 0.30042542266699357 1103 4 0.29407680090935173 1103 5 0.2885588254287512 1103 6 0.24110885697245976 1103 7 0.35559188328475244 1103 8 0.28242120258531145 1103 9 0.4408807527179093 1103 10-11 0.4171542332573779 1103 12-13 0.47556618225920744 1103 14-15 0.5408630244805224 1103 16-17 0.550061647775685 1103 18-19 0.523129945010993 1103 20-21 0.4647721681048367 1103 22-23 0.5359724789726741 1103 24-25 0.48632134782680225 1103 26-27 0.5879969014933835 1103 28-29 0.5842986839471891 1103 30-31 0.6297024363873263 1103 32-33 0.7263140101129792 1103 34-35 0.6291656697976507 1103 36-37 0.6604736097105857 1103 38-39 0.6664175202151625 1103 40-41 0.7653365360069202 1103 42-43 0.7231244968855108 1103 44-45 0.5884847838027483 1103 46-47 0.6470062900463418 1103 48-49 0.8290032657540252 1103 50-51 0.8097445189601657 1103 52-53 0.8254208473358062 1103 54-55 0.7754621953594665 1103 56-57 0.8765023115063357 1103 58-59 0.9954957893675029 1103 60-61 0.9069259799193112 1103 62-63 0.9888282090521692 1103 64-65 0.8671186172380239 1103 66-67 0.8623258353401972 1103 68-69 0.6881197089270508 1103 70-71 0.441720539834602 1103 72-73 0.4772723116502853 1103 74-75 0.3678475231085123 1103 76-77 0.3086722417709389 1103 78-79 0.47202325638659204 1103 80-81 0.591680563913588 1103 82-83 0.4543733518833548 1103 84-85 0.3594027532289381 1103 86-87 0.5262835040151508 1103 88-89 0.49682712313555655 1103 90-91 0.5032592579655777 1103 92-93 0.5006253326149164 1103 94-95 0.5393582912101067 1103 96-97 0.7021657621039665 1103 98-99 0.8459754086229161 1103 100-101 0.48196952385730896 1104 1 0.26979294611903626 1104 2 0.2777355196656579 1104 3 0.23172768630192664 1104 4 0.21141930468377268 1104 5 0.2359318549785101 1104 6 0.2614040496076413 1104 7 0.2179241948560815 1104 8 0.18118987770058226 1104 9 0.3134482220523367 1104 10-11 0.3548507699416774 1104 12-13 0.4360119195799541 1104 14-15 0.5369605036258989 1104 16-17 0.5211095638386993 1104 18-19 0.47237099442665453 1104 20-21 0.41315946165553186 1104 22-23 0.39877023872135453 1104 24-25 0.34674745054184086 1104 26-27 0.5509632392645187 1104 28-29 0.5703845475394189 1104 30-31 0.6024008030931327 1104 32-33 0.7107825286597347 1104 34-35 0.4894780315929026 1104 36-37 0.48013318758561496 1104 38-39 0.5473131759474512 1104 40-41 0.6214254877604404 1104 42-43 0.7893588209721614 1104 44-45 0.5202702239483372 1104 46-47 0.49399552341983366 1104 48-49 0.569952030235342 1104 50-51 0.6272482298371003 1104 52-53 0.7511518392582985 1104 54-55 0.6960243296728734 1104 56-57 0.738389572014988 1104 58-59 0.5519705071121024 1104 60-61 0.4003769810935758 1104 62-63 0.5361681330046117 1104 64-65 0.674618845990711 1104 66-67 0.5464767053629167 1104 68-69 0.5531396104108914 1104 70-71 0.30113593692385265 1104 72-73 0.18466686936967136 1104 74-75 0.16454274573571226 1104 76-77 0.2678748988604909 1104 78-79 0.3954902956659687 1104 80-81 0.4627359779813709 1104 82-83 0.31342515928376 1104 84-85 0.3878970776205861 1104 86-87 0.4726555499283549 1104 88-89 0.36455285120452885 1104 90-91 0.39278861201882265 1104 92-93 0.423064065020025 1104 94-95 0.2618308917214982 1104 96-97 0.2869544708712972 1104 98-99 0.6661581947289861 1104 100-101 0.2898335964684957 1105 1 0.2650048759362704 1105 2 0.30829437356581835 1105 3 0.2293588981359136 1105 4 0.19858452953190664 1105 5 0.2376254261038966 1105 6 0.23322160095073485 1105 7 0.2259731112347012 1105 8 0.2663668611045651 1105 9 0.33503035042343043 1105 10-11 0.45413550181748974 1105 12-13 0.4196085437404662 1105 14-15 0.5662494556082152 1105 16-17 0.5026105703371755 1105 18-19 0.44606326819177866 1105 20-21 0.3520028084764206 1105 22-23 0.38108307822646736 1105 24-25 0.53529118208656 1105 26-27 0.39437713957048715 1105 28-29 0.5029469998942986 1105 30-31 0.5792684152192749 1105 32-33 0.6850556659925644 1105 34-35 0.7473612458274985 1105 36-37 0.7547123594250351 1105 38-39 0.5910174466092286 1105 40-41 0.6394186827619208 1105 42-43 0.6584769169977065 1105 44-45 0.6744412801605222 1105 46-47 0.5560084220801542 1105 48-49 0.5489327055875179 1105 50-51 0.42099639083507867 1105 52-53 0.40214804853381025 1105 54-55 0.47327844704098254 1105 56-57 0.5048075984425608 1105 58-59 0.5234952309777015 1105 60-61 0.33425122929112305 1105 62-63 0.48169509653662246 1105 64-65 0.45130444682760995 1105 66-67 0.3080903217484767 1105 68-69 0.2065732229756314 1105 70-71 0.15347305893407182 1105 72-73 0.352539450410152 1105 74-75 0.1699487820237131 1105 76-77 0.3500638380043455 1105 78-79 0.47978265383322594 1105 80-81 0.6024790613373803 1105 82-83 0.7064562093162863 1105 84-85 0.6864154946690775 1105 86-87 0.6276658360540317 1105 88-89 0.5276181965130533 1105 90-91 0.32570716273473366 1105 92-93 0.4418097535393066 1105 94-95 0.6692445192869627 1105 96-97 0.5579983974500422 1105 98-99 0.2661190036380212 1105 100-101 0.05498946091154977 1106 1 0.1723945583715043 1106 2 0.1718487047766537 1106 3 0.2350080229853333 1106 4 0.1026216614270723 1106 5 -0.06684244792301541 1106 6 0.10499634964230609 1106 7 0.1886330577842088 1106 8 0.20711438345152544 1106 9 0.03235997697861137 1106 10-11 0.06362005381529912 1106 12-13 0.06282062223574769 1106 14-15 0.10813495210052082 1106 16-17 -0.05194733689383213 1106 18-19 0.352926480991556 1106 20-21 0.23225143425210604 1106 22-23 0.32188602508978903 1106 24-25 0.25401214115846216 1106 26-27 0.22097995837533801 1106 28-29 0.24644474242084158 1106 30-31 0.35231647257732845 1106 32-33 0.408747305748399 1106 34-35 0.1042002262188646 1106 36-37 -0.2976534322597644 1106 38-39 -0.03911046001437768 1106 40-41 0.28072304434235207 1106 42-43 0.4587437898401916 1106 44-45 0.3517691098478224 1106 46-47 0.03677130638210713 1106 48-49 0.0807761148785957 1106 50-51 0.1599403386803644 1106 52-53 0.08231904202459361 1106 54-55 -0.305829167629561 1106 56-57 -0.07178452773299426 1106 58-59 -0.0014847124336192508 1106 60-61 -0.3788870384270062 1106 62-63 -0.29356424717636287 1106 64-65 -0.6224641925131671 1106 66-67 -0.22716587111871434 1106 68-69 -0.9065240457851864 1106 70-71 -0.8356565230790309 1106 72-73 -0.4695443089411562 1106 74-75 -0.4178309346279363 1106 76-77 -0.3885920415498276 1106 78-79 -0.4645023572516749 1106 80-81 -1.0205298951632322 1106 82-83 -0.5125414927174639 1106 84-85 -0.3599729274278438 1106 86-87 -0.03759665850474647 1106 88-89 -0.0849846805001988 1106 90-91 -0.24147298212824708 1106 92-93 -0.41703456937050376 1106 94-95 -0.31609272244491393 1106 96-97 -0.1777695231435814 1106 98-99 -0.13945172968653807 1106 100-101 -0.1764470590362155 1107 1 0.15343436963366486 1107 2 0.178978842723577 1107 3 0.23797220386911278 1107 4 0.07226231820269646 1107 5 0.10962292509469052 1107 6 0.1352627765464689 1107 7 0.09620047846723168 1107 8 0.20737096773740404 1107 9 0.2890961597457604 1107 10-11 0.21365223642217046 1107 12-13 0.26233118364066854 1107 14-15 0.29710596020503033 1107 16-17 0.38967998851865815 1107 18-19 0.3121724688097487 1107 20-21 0.37862945090190436 1107 22-23 0.3996460906070709 1107 24-25 0.2995152166636146 1107 26-27 0.3196103192212547 1107 28-29 0.366904239921368 1107 30-31 0.3384093914020241 1107 32-33 0.4332862930356427 1107 34-35 0.5036655190931114 1107 36-37 0.5362337257530712 1107 38-39 0.4486453729265918 1107 40-41 0.32762994519487876 1107 42-43 0.5066286287017547 1107 44-45 0.40383906506766465 1107 46-47 0.4869411103311734 1107 48-49 0.5323137422315583 1107 50-51 0.4589756118192767 1107 52-53 0.6232664002959041 1107 54-55 0.676878504102838 1107 56-57 0.45695090869821087 1107 58-59 0.550315823024853 1107 60-61 0.4487455489043164 1107 62-63 0.46184424652736666 1107 64-65 0.4497376738275811 1107 66-67 0.2402231301936375 1107 68-69 0.7097947899307435 1107 70-71 0.5113061651328685 1107 72-73 0.4632103218479564 1107 74-75 0.5349728967301957 1107 76-77 0.5028038696311867 1107 78-79 0.7271418734212283 1107 80-81 0.7457395396250384 1107 82-83 0.5538518513684494 1107 84-85 0.6663357901766709 1107 86-87 0.6825211022870725 1107 88-89 0.4537862194106417 1107 90-91 0.3832048787427098 1107 92-93 0.019133734393232515 1107 94-95 -0.06558324798562865 1107 96-97 -0.021777422283040693 1107 98-99 -0.014414099829096472 1107 100-101 -0.042343200629666455 1108 1 0.21763837356300542 1108 2 0.2747714993426378 1108 3 0.18235534378238683 1108 4 0.1557321546670707 1108 5 0.1637759109787993 1108 6 0.14371999865900165 1108 7 0.24624191691772523 1108 8 0.2280198694965847 1108 9 0.22613116548620837 1108 10-11 0.23181056542884448 1108 12-13 0.2078844113303262 1108 14-15 0.38076854640310387 1108 16-17 0.22110977350185124 1108 18-19 0.35521732958873997 1108 20-21 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100-101 0.8177342399581633 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 35.0 4 56.0 5 128.0 6 272.0 7 766.0 8 1620.0 9 2883.0 10 4802.0 11 7294.0 12 10079.0 13 14495.0 14 18856.0 15 24411.0 16 29553.0 17 35620.0 18 35806.0 19 33835.0 20 35227.0 21 37566.0 22 40671.0 23 46108.0 24 51882.0 25 60110.0 26 70401.0 27 82385.0 28 96862.0 29 112260.0 30 129641.0 31 149306.0 32 173521.0 33 204040.0 34 238673.0 35 275982.0 36 289968.0 37 219461.0 38 56595.0 39 1034.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 40.893381174347475 20.438608639698295 15.827385032850513 22.84062515310372 2 16.87980990584442 21.216979264315242 38.31248598588187 23.590724843958462 3 20.07088981063131 24.154653667629557 29.963019714589045 25.81143680715009 4 14.488657734509628 17.612983839639348 35.60267370622794 32.29568471962308 5 14.176482034286739 36.98047530727879 34.225351677659575 14.617690980774903 6 33.053764610619446 36.250577210847744 16.556972494866343 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26.753322554873126 28.67959618965615 22.18893012787945 30-31 22.504047709152857 26.463569460309305 28.704999253531838 22.327383577006003 32-33 22.445834765510625 26.370405604182075 28.642022029876475 22.54173760043083 34-35 22.824353956106766 26.520260722772846 28.505794000150853 22.149591320969535 36-37 22.86310138519244 26.29279034087994 28.519043454544263 22.325064819383357 38-39 22.48619268362689 26.51895911583794 28.47767682160901 22.517171378926154 40-41 22.859383198049443 26.38873028610228 28.425492268757136 22.326394247091137 42-43 22.614077350142225 26.437934271648462 28.33809666269899 22.609891715510322 44-45 22.68602057745014 26.6040147650274 28.306753339288367 22.4032113182341 46-47 22.814690339158872 26.77644956594902 27.79708564940994 22.61177444548217 48-49 22.79399368954717 26.722904459404667 27.872079660304905 22.611022190743256 50-51 22.954243237519226 26.94096189077493 27.63857207391231 22.466222797793538 52-53 23.029179382665042 26.853488166647182 27.535937523507958 22.58139492717981 54-55 22.88085017901734 26.877348143878944 27.617258189643035 22.624543487460684 56-57 22.830622708757442 27.065141788445136 27.54141548109391 22.562820021703516 58-59 22.888121974826856 26.809375177213855 27.573048757294462 22.729454090664827 60-61 22.83563774035021 26.95224571185866 27.671266222180556 22.54085032561057 62-63 22.590827044290833 26.98474697429642 27.768712143744693 22.655713837668056 64-65 22.531611094330046 26.884928557017247 27.69844383569676 22.88501651295595 66-67 22.574800714989887 26.880884590702625 27.5688318572088 22.975482837098692 68-69 22.676893063581026 26.768564148985767 27.672012037741972 22.882530749691234 70-71 22.66117070216283 26.761405830171608 27.5514860900276 23.025937377637955 72-73 22.71192076867318 26.847007202742684 27.402614837472473 23.038457191111668 74-75 22.64738116979084 27.086127766802576 27.10645793333634 23.160033130070246 76-77 22.60652795089281 26.852272985915093 27.51026441946959 23.03093464372251 78-79 22.665396706358713 26.934268752456887 27.45664215859304 22.94369238259136 80-81 22.631350018854874 27.044471470304295 27.285968210001858 23.038210300838973 82-83 22.727287632222023 26.979513603107186 27.29575278910617 22.997445975564617 84-85 22.7306685904923 26.881348118241977 27.30628082131666 23.081702469949068 86-87 22.68039586604039 26.794468430422054 27.31145449540814 23.213681208129426 88-89 22.605797497844016 26.978677801076266 27.152911739795222 23.2626129612845 90-91 22.6631629566575 26.85073682265352 27.210250955833466 23.275849264855516 92-93 22.555723410969463 26.626701561389687 27.21682307310686 23.600751954533987 94-95 22.469442038727646 26.734324638676338 27.165752708601364 23.63048061399465 96-97 22.51490718140951 26.62055923774606 27.089522557419222 23.775011023425215 98-99 22.45798894338776 26.592732170629287 26.974820080745943 23.97445880523701 100-101 22.768922923385926 26.436242637305323 27.089469916844973 23.70536452246378 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 430.0 1 307.5 2 275.5 3 377.0 4 321.5 5 333.0 6 662.0 7 1498.5 8 2338.5 9 2562.0 10 2220.5 11 1783.0 12 1756.5 13 1964.0 14 2300.5 15 2532.5 16 2663.5 17 2870.0 18 3112.0 19 3510.0 20 3988.0 21 4629.5 22 5439.5 23 6602.5 24 8264.0 25 10568.0 26 13793.5 27 17580.0 28 20860.0 29 24164.0 30 28051.5 31 33149.5 32 38880.5 33 42410.0 34 45634.0 35 49944.0 36 55641.0 37 61317.0 38 65115.0 39 68295.0 40 72080.0 41 77906.0 42 83351.0 43 87986.5 44 92451.5 45 96058.5 46 98130.5 47 99630.5 48 99785.0 49 99272.5 50 99555.0 51 96949.5 52 93513.5 53 92457.5 54 91543.5 55 85449.5 56 76679.5 57 69290.0 58 62489.5 59 54918.5 60 45209.5 61 36891.5 62 30822.5 63 24459.0 64 19350.0 65 16019.5 66 12621.0 67 9758.0 68 7304.0 69 5201.0 70 3990.5 71 3112.0 72 2442.0 73 2026.5 74 1659.0 75 1300.5 76 940.5 77 614.0 78 396.5 79 245.5 80 161.0 81 101.5 82 61.0 83 40.5 84 25.0 85 10.0 86 3.0 87 3.5 88 5.0 89 4.0 90 1.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 3.8577166098232126E-4 2 0.04127756772510837 3 5.786574914734819E-4 4 0.0 5 0.07912176766747409 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 1.1573149829469638E-4 26-27 0.007831164717941121 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.009972197436393004 36-37 1.543086643929285E-4 38-39 0.003973448108117909 40-41 0.00489930009447548 42-43 2.8932874573674096E-4 44-45 1.9288583049116063E-5 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 9.644291524558032E-5 68-69 0.0010222949016031513 70-71 7.715433219646425E-5 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.001523798060880169 82-83 9.06563403308455E-4 84-85 0.005767286331685703 86-87 0.0026039587116306684 88-89 9.644291524558032E-5 90-91 0.003761273694577632 92-93 0.0011187378168487315 94-95 7.715433219646425E-5 96-97 0.0 98-99 9.644291524558032E-5 100-101 1.9288583049116063E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2592207.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.9573345313499 #Duplication Level Percentage of deduplicated Percentage of total 1 76.63995603849503 42.88567658514017 2 13.404631482648346 15.001748962880368 3 4.307126851003283 7.230460142116511 4 1.9113384881522377 4.2781362873671736 5 1.0069916551810771 2.817428445962264 6 0.607907361426274 2.041012535244015 7 0.3902261396337052 1.5285210246852354 8 0.27794576933251497 1.2442483516890364 9 0.2002815220100745 1.008649811480909 >10 1.1088999082804047 11.481784975971003 >50 0.09522375890390752 3.6764526060048217 >100 0.046018658214502746 4.662837773737725 >500 0.002485704037454702 0.8753995938262895 >1k 8.285680124739741E-4 0.7175286161791995 >5k 1.3809466874566237E-4 0.550114287715225 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7675 0.29607974980393154 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6563 0.2531819411026974 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4758 0.18355015629538846 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 3.8577166098232126E-5 0.0 8 0.0 0.0 0.0 3.8577166098232126E-5 0.0 9 0.0 0.0 0.0 7.715433219646425E-5 0.0 10-11 0.0 0.0 0.0 1.1573149829469638E-4 0.0 12-13 0.0 0.0 0.0 1.543086643929285E-4 0.0 14-15 0.0 0.0 0.0 1.9288583049116063E-4 0.0 16-17 3.8577166098232126E-5 0.0 0.0 1.9288583049116063E-4 0.0 18-19 7.715433219646425E-5 0.0 0.0 1.9288583049116063E-4 0.0 20-21 7.715433219646425E-5 0.0 0.0 1.9288583049116063E-4 0.0 22-23 1.1573149829469638E-4 0.0 0.0 2.3146299658939276E-4 0.0 24-25 1.7359724744204455E-4 0.0 0.0 3.471944948840891E-4 0.0 26-27 2.3146299658939276E-4 0.0 0.0 6.558118236699461E-4 0.0 28-29 2.3146299658939276E-4 0.0 0.0 0.001581663810027517 0.0 30-31 2.3146299658939276E-4 0.0 0.0 0.006056615077422443 0.0 32-33 2.3146299658939276E-4 1.9288583049116063E-5 0.0 0.015643040852833127 0.0 34-35 2.507515796385088E-4 3.8577166098232126E-5 0.0 0.03213477935982736 0.0 36-37 2.7004016268762486E-4 3.8577166098232126E-5 0.0 0.05863729246931283 0.0 38-39 2.7004016268762486E-4 3.8577166098232126E-5 0.0 0.10057067201809114 0.0 40-41 2.7004016268762486E-4 3.8577166098232126E-5 0.0 0.1442207354582408 0.0 42-43 2.7004016268762486E-4 3.8577166098232126E-5 0.0 0.1927315218267677 0.0 44-45 2.7004016268762486E-4 3.8577166098232126E-5 0.0 0.2548021820788232 0.0 46-47 2.7004016268762486E-4 5.786574914734819E-5 0.0 0.3298540587229338 0.0 48-49 2.7004016268762486E-4 7.715433219646425E-5 0.0 0.41059606736653365 0.0 50-51 2.7004016268762486E-4 7.715433219646425E-5 0.0 0.4984555631552573 0.0 52-53 2.7004016268762486E-4 7.715433219646425E-5 0.0 0.5875302396760752 0.0 54-55 2.7004016268762486E-4 7.715433219646425E-5 0.0 0.686210630555353 0.0 56-57 2.7004016268762486E-4 7.715433219646425E-5 0.0 0.8041217387345996 0.0 58-59 2.7004016268762486E-4 7.715433219646425E-5 0.0 0.931677138438404 0.0 60-61 3.08617328785857E-4 7.715433219646425E-5 0.0 1.072271620283411 0.0 62-63 3.08617328785857E-4 7.715433219646425E-5 0.0 1.2113230154844887 0.0 64-65 3.08617328785857E-4 7.715433219646425E-5 0.0 1.363760687321653 0.0 66-67 3.08617328785857E-4 7.715433219646425E-5 0.0 1.5383995182483496 0.0 68-69 3.08617328785857E-4 7.715433219646425E-5 0.0 1.7409296402640684 0.0 70-71 3.08617328785857E-4 7.715433219646425E-5 0.0 1.9515223899943175 0.0 72-73 3.08617328785857E-4 7.715433219646425E-5 0.0 2.167419500063074 0.0 74-75 3.08617328785857E-4 7.715433219646425E-5 0.0 2.3968571954323092 0.0 76-77 3.08617328785857E-4 7.715433219646425E-5 0.0 2.647820949484358 0.0 78-79 3.08617328785857E-4 7.715433219646425E-5 0.0 2.9240141701646514 0.0 80-81 3.08617328785857E-4 7.715433219646425E-5 0.0 3.219013759317832 0.0 82-83 3.2790591183497306E-4 7.715433219646425E-5 0.0 3.5303507783136148 0.0 84-85 3.8577166098232126E-4 7.715433219646425E-5 0.0 3.8436166556143085 0.0 86-87 3.8577166098232126E-4 7.715433219646425E-5 0.0 4.173721465916881 0.0 88-89 3.8577166098232126E-4 7.715433219646425E-5 0.0 4.5274547904546205 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4215 0.0 53.816277 1 GTATCAA 6965 0.0 41.23905 1 ATCAACG 9310 0.0 30.4308 3 TATCAAC 9495 0.0 30.099825 2 TCAACGC 9560 0.0 29.58529 4 CAACGCA 9775 0.0 28.983194 5 TGGTATC 1860 0.0 28.37883 2 AACGCAG 10270 0.0 27.748352 6 GCACCGT 595 0.0 25.54403 6 ACGCAGA 11895 0.0 23.837807 7 GTGGTAT 2370 0.0 23.676992 1 CGCAGAG 12010 0.0 23.649097 8 TGCACCG 620 0.0 21.46758 5 GTACATG 7700 0.0 21.245188 1 GCAGAGT 13185 0.0 21.073278 9 GAGTACT 8270 0.0 21.019964 12-13 TACATGG 7480 0.0 20.852432 2 AGTACTT 8770 0.0 19.87572 12-13 AGAGTAC 12165 0.0 19.716764 10-11 CAGAGTA 12610 0.0 19.604782 10-11 >>END_MODULE