FastQCFastQC Report
Thu 26 May 2016
SRR938293_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938293_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2611959
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80500.3081977933037999No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58680.22465896287039727No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43850.16788165511020656No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA36100.050.6598661
GTATCAA63600.045.0046461
ATCAACG82450.034.1229443
TATCAAC85300.033.2614142
TCAACGC86000.032.7143754
CAACGCA87800.032.0436975
AACGCAG90850.030.9120676
ACGCAGA106450.026.2445377
CGCAGAG106450.026.1106368
GTACATG73400.024.2020851
TACATGG70700.023.5940822
GCAGAGT117900.023.2520359
GTGGTAT21050.022.8512151
TGGTATC18950.022.3201052
TAGCGCG1754.034602E-621.7253784
ACATGGG75350.021.6965453
GAGTACT73000.021.44116212-13
GGACCGA7950.020.3225066
AGTACTT77200.020.21314212-13
GTACTTT77850.019.77111814-15
Produced by FastQC (version 0.11.5)