##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938293_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2611959 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.66282395703761 31.0 31.0 34.0 30.0 34.0 2 31.84446425077882 33.0 31.0 34.0 30.0 34.0 3 31.91610970922591 33.0 31.0 34.0 30.0 34.0 4 35.577722697791195 37.0 35.0 37.0 33.0 37.0 5 35.585992352866185 37.0 35.0 37.0 33.0 37.0 6 35.602897671824095 37.0 35.0 37.0 33.0 37.0 7 35.541805212103256 37.0 35.0 37.0 33.0 37.0 8 35.50866801507987 37.0 35.0 37.0 33.0 37.0 9 37.09501565683075 39.0 37.0 39.0 33.0 39.0 10-11 36.9718942755227 39.0 37.0 39.0 33.0 39.0 12-13 36.82173246210986 39.0 37.0 39.0 33.0 39.0 14-15 37.92621591686546 40.0 38.0 41.0 33.0 41.0 16-17 37.755319666196904 40.0 37.0 41.0 33.0 41.0 18-19 37.611957538384026 40.0 37.0 41.0 32.0 41.0 20-21 37.59165304662133 39.5 37.0 41.0 32.0 41.0 22-23 37.7073568153252 40.0 37.0 41.0 32.0 41.0 24-25 37.637795999094934 40.0 37.0 41.0 32.0 41.0 26-27 37.455720017044676 40.0 37.0 41.0 32.0 41.0 28-29 37.27145027927314 39.0 37.0 41.0 31.5 41.0 30-31 37.03199437663455 39.0 36.0 41.0 31.0 41.0 32-33 36.75714396742062 39.0 36.0 40.0 30.5 41.0 34-35 36.502118141976965 39.0 35.0 40.0 30.0 41.0 36-37 36.23162385014466 38.0 35.0 40.0 30.0 41.0 38-39 35.92980632544385 38.0 35.0 40.0 29.5 41.0 40-41 35.843765924350265 38.0 35.0 40.0 29.0 41.0 42-43 35.628271921573045 38.0 35.0 40.0 27.5 41.0 44-45 35.3394469438456 38.0 34.5 40.0 27.0 41.0 46-47 34.9583726237663 38.0 34.0 40.0 25.5 41.0 48-49 34.585068716622274 38.0 33.5 40.0 24.0 41.0 50-51 33.82590748935952 37.5 32.5 39.5 23.5 40.5 52-53 33.855145888584005 37.5 33.0 39.5 23.5 40.5 54-55 33.925086113526284 38.0 33.0 40.0 23.0 41.0 56-57 33.588895154939266 37.5 33.0 40.0 22.0 41.0 58-59 33.14650249104216 37.0 32.0 40.0 19.5 41.0 60-61 32.65828923807763 36.0 31.0 39.0 17.5 41.0 62-63 32.07259819162552 36.0 31.0 39.0 13.5 40.5 64-65 31.56659178034571 35.0 30.5 39.0 8.0 40.0 66-67 31.00129519644068 35.0 29.5 38.0 6.5 40.0 68-69 30.35993042004105 34.5 29.0 38.0 2.0 40.0 70-71 29.70982603478845 34.0 28.0 37.0 2.0 39.5 72-73 29.057297606891993 34.0 27.5 36.5 2.0 39.0 74-75 28.329308385009107 34.0 26.0 36.0 2.0 39.0 76-77 27.572249985547245 33.0 25.5 35.5 2.0 38.0 78-79 26.76607155778479 32.5 24.0 35.0 2.0 37.0 80-81 25.968945530921427 32.0 21.0 35.0 2.0 36.5 82-83 25.217452111614307 31.5 19.0 35.0 2.0 36.0 84-85 24.456134648361633 31.0 14.0 34.0 2.0 35.5 86-87 23.704708420002 31.0 7.0 34.0 2.0 35.0 88-89 22.926334218875567 30.0 2.0 34.0 2.0 35.0 90-91 22.095966858591577 29.5 2.0 34.0 2.0 35.0 92-93 21.207842657560857 29.0 2.0 34.0 2.0 35.0 94-95 20.292627679071533 28.0 2.0 34.0 2.0 35.0 96-97 19.19460048951764 26.0 2.0 34.0 2.0 35.0 98-99 17.91930577777063 24.0 2.0 33.0 2.0 35.0 100-101 14.980283572598191 2.0 2.0 30.0 2.0 33.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.38532721164788697 1101 2 0.4396616320296083 1101 3 0.3839207581583146 1101 4 0.2510277787848523 1101 5 0.3044820838031086 1101 6 0.29842575220219913 1101 7 0.3200006571640728 1101 8 0.27759159858963045 1101 9 0.3827174066675596 1101 10-11 0.43319102628282025 1101 12-13 0.41072875974213474 1101 14-15 0.5611791859911079 1101 16-17 0.7267213616906361 1101 18-19 0.7573453216798853 1101 20-21 0.8152817692919783 1101 22-23 0.6326670415257141 1101 24-25 0.738477232439763 1101 26-27 0.7967643229372285 1101 28-29 0.8725568280165632 1101 30-31 0.7974889755990517 1101 32-33 0.9193160959777913 1101 34-35 0.9434524418563441 1101 36-37 1.0508040707904343 1101 38-39 1.1417163808786341 1101 40-41 1.2223611645914474 1101 42-43 1.1518836172058258 1101 44-45 1.2011693325123645 1101 46-47 1.4665592113345056 1101 48-49 1.4349735798728034 1101 50-51 1.5741178422330577 1101 52-53 1.6532362125121551 1101 54-55 1.7838973407070853 1101 56-57 1.885678052933109 1101 58-59 2.0381319502571458 1101 60-61 2.0938915820683732 1101 62-63 2.175574796760401 1101 64-65 2.294229641929711 1101 66-67 2.482185238089098 1101 68-69 2.721629912834512 1101 70-71 2.755582012033212 1101 72-73 2.962785662375474 1101 74-75 3.1748568821532572 1101 76-77 3.5532014894084227 1101 78-79 3.804645108129588 1101 80-81 4.154728374822074 1101 82-83 4.186704829879822 1101 84-85 4.277877097627034 1101 86-87 4.71699082928761 1101 88-89 4.97726868768444 1101 90-91 5.112762804034013 1101 92-93 5.141754804146334 1101 94-95 5.482350723504311 1101 96-97 5.710202631566059 1101 98-99 5.837813476874199 1101 100-101 5.587292901917213 1102 1 0.4099854139895349 1102 2 0.39963710751953485 1102 3 0.37065447417240094 1102 4 0.3251205697767716 1102 5 0.2335420239303616 1102 6 0.31770658404620633 1102 7 0.3543290888040076 1102 8 0.3555010365152427 1102 9 0.3843046013010465 1102 10-11 0.4072902879806293 1102 12-13 0.5125160382274103 1102 14-15 0.6637307831859189 1102 16-17 0.6760248517006104 1102 18-19 0.7331765667399637 1102 20-21 0.7272629229600156 1102 22-23 0.5878393548191028 1102 24-25 0.40416700968705044 1102 26-27 0.4723729971056301 1102 28-29 0.3717106896375739 1102 30-31 0.4748365851277754 1102 32-33 0.5615890036135411 1102 34-35 0.8038047156410002 1102 36-37 0.8459995449327451 1102 38-39 0.6922059352341705 1102 40-41 0.9741676145482856 1102 42-43 1.1793121907734658 1102 44-45 1.307214576115726 1102 46-47 1.2792809399648561 1102 48-49 1.3582346177740234 1102 50-51 1.498166728683394 1102 52-53 1.5677472250118498 1102 54-55 1.671897844459565 1102 56-57 1.738083791860987 1102 58-59 1.7901765061474677 1102 60-61 1.8736767492853161 1102 62-63 2.210242940459274 1102 64-65 2.1189761881235967 1102 66-67 2.115105970337705 1102 68-69 2.4468735803664323 1102 70-71 2.5074626612000976 1102 72-73 2.6711006798677737 1102 74-75 2.835354720081863 1102 76-77 2.8469923925585334 1102 78-79 3.1798221166955223 1102 80-81 3.6031894698366393 1102 82-83 3.9773063702286855 1102 84-85 4.016674353833835 1102 86-87 4.355053767330336 1102 88-89 4.4801476170339605 1102 90-91 4.872922480734015 1102 92-93 4.826181686774145 1102 94-95 5.057665328154879 1102 96-97 5.1691620458014 1102 98-99 5.425122283558384 1102 100-101 5.488526132895743 1103 1 0.3523105134021094 1103 2 0.40583101562506485 1103 3 0.2904140632462777 1103 4 0.23781255413710767 1103 5 0.1971261204151773 1103 6 0.2873938115849981 1103 7 0.29639070067150897 1103 8 0.27083127770648474 1103 9 0.4180835485161367 1103 10-11 0.32066908266947536 1103 12-13 0.44391082632412093 1103 14-15 0.5724091993682734 1103 16-17 0.5579404243607513 1103 18-19 0.6554035228943818 1103 20-21 0.6867689449379455 1103 22-23 0.5859582670343215 1103 24-25 0.5653466067513833 1103 26-27 0.652692896475763 1103 28-29 0.6769405922746401 1103 30-31 0.6303437869472717 1103 32-33 0.8330478922735409 1103 34-35 0.9165256238068764 1103 36-37 0.9349146265124446 1103 38-39 1.102352650717556 1103 40-41 1.1057601353515807 1103 42-43 1.3571402730835445 1103 44-45 1.3455451211109732 1103 46-47 1.4507896129448241 1103 48-49 1.4576533065060246 1103 50-51 1.6515394993134507 1103 52-53 1.6455810036469387 1103 54-55 1.738806643955165 1103 56-57 1.8586547040845147 1103 58-59 2.053984892178306 1103 60-61 2.200108165532164 1103 62-63 2.328852782252298 1103 64-65 2.354474619163213 1103 66-67 2.3735274276397185 1103 68-69 2.6364372626790065 1103 70-71 2.849549997567312 1103 72-73 3.0564099877148614 1103 74-75 3.2685252529665654 1103 76-77 3.5264107639610067 1103 78-79 3.5412049086959065 1103 80-81 3.9805248330384835 1103 82-83 4.185678069221343 1103 84-85 4.542309507077981 1103 86-87 4.695841353191383 1103 88-89 4.858485429395454 1103 90-91 5.146415919816111 1103 92-93 5.203654255977995 1103 94-95 5.267928389125846 1103 96-97 5.354883309792509 1103 98-99 5.4176476126967295 1103 100-101 5.5124335309393295 1104 1 0.4589734917872299 1104 2 0.3988956387679856 1104 3 0.2958735374942201 1104 4 0.2664571258396293 1104 5 0.23680076948058826 1104 6 0.2721715589080418 1104 7 0.32178636495157775 1104 8 0.333491270404096 1104 9 0.3861489807884695 1104 10-11 0.46966729196495294 1104 12-13 0.5181487654917518 1104 14-15 0.6015582758987676 1104 16-17 0.7935388691128864 1104 18-19 0.7952156481031167 1104 20-21 0.7819782540729179 1104 22-23 0.735804987582263 1104 24-25 0.7767222455843452 1104 26-27 0.7820159231861297 1104 28-29 0.7750161791210246 1104 30-31 0.8000768222849928 1104 32-33 0.9265558804036047 1104 34-35 0.992503001560074 1104 36-37 1.1168517354121619 1104 38-39 1.1747169496018728 1104 40-41 1.20923179357127 1104 42-43 1.2744845434865013 1104 44-45 1.3039122524539124 1104 46-47 1.5743960771808005 1104 48-49 1.708357059450492 1104 50-51 1.639035858231118 1104 52-53 1.6432728987999496 1104 54-55 1.844649809896481 1104 56-57 2.082033714458568 1104 58-59 2.055049971973638 1104 60-61 2.265588100639988 1104 62-63 2.4201193554982297 1104 64-65 2.439325807622602 1104 66-67 2.595088172524253 1104 68-69 2.703038537361248 1104 70-71 2.9318084319828195 1104 72-73 3.028847753020809 1104 74-75 3.190088943587842 1104 76-77 3.2454118785367605 1104 78-79 3.611617309312056 1104 80-81 4.099646697535604 1104 82-83 4.273492567707766 1104 84-85 4.378605117955512 1104 86-87 4.585684642524932 1104 88-89 4.899864623340736 1104 90-91 5.082881245586783 1104 92-93 5.141147871441266 1104 94-95 5.3904240071844 1104 96-97 5.649099324081732 1104 98-99 5.9281024496217505 1104 100-101 5.8145958281386285 1105 1 0.3833446187825622 1105 2 0.3597519404897618 1105 3 0.34660536378750706 1105 4 0.3282614557607957 1105 5 0.30586637033558617 1105 6 0.3135966530367611 1105 7 0.23698386367691882 1105 8 0.29493685713978834 1105 9 0.36498043644991895 1105 10-11 0.38700842523553547 1105 12-13 0.3566336852862335 1105 14-15 0.5075885809346588 1105 16-17 0.6199927166243597 1105 18-19 0.607912178577287 1105 20-21 0.6492525289876099 1105 22-23 0.6010360686898437 1105 24-25 0.5679617173849394 1105 26-27 0.6538340537505078 1105 28-29 0.8018316500587872 1105 30-31 0.7868997205671349 1105 32-33 0.7910121154508758 1105 34-35 0.806841208015733 1105 36-37 1.104347629536953 1105 38-39 1.1769860278267643 1105 40-41 1.2136522278380468 1105 42-43 1.256052840737155 1105 44-45 1.2697089025500077 1105 46-47 1.347504433065808 1105 48-49 1.3876452477667627 1105 50-51 1.2506823877247868 1105 52-53 1.4317393727968906 1105 54-55 1.4911959109083668 1105 56-57 1.588768023887127 1105 58-59 1.669614369255676 1105 60-61 1.9256409377384713 1105 62-63 2.1173700737199823 1105 64-65 2.3549636155019584 1105 66-67 2.4354513770188966 1105 68-69 2.547412569110083 1105 70-71 2.6944928899582656 1105 72-73 2.7584520324458417 1105 74-75 2.7830240192830225 1105 76-77 2.9238011253108915 1105 78-79 3.1710371675306703 1105 80-81 3.610568509415433 1105 82-83 3.8330400757479275 1105 84-85 4.004706534298613 1105 86-87 4.266378204512616 1105 88-89 4.6279383305415855 1105 90-91 4.913298978555368 1105 92-93 4.952980207108208 1105 94-95 5.265321237740935 1105 96-97 5.551273293078086 1105 98-99 5.668913133231587 1105 100-101 5.176295059910991 1106 1 0.1910125048163458 1106 2 0.17801047605958686 1106 3 0.18840214584794168 1106 4 0.04651627374794742 1106 5 0.13727325232463983 1106 6 -0.003759253458596845 1106 7 0.07750923246112507 1106 8 0.1531727898069306 1106 9 0.15934235055888735 1106 10-11 0.037736327924086766 1106 12-13 0.0495554078653484 1106 14-15 0.3612254920695577 1106 16-17 0.08495078229174169 1106 18-19 0.2810574765787166 1106 20-21 0.2997220596799721 1106 22-23 0.2545239047593171 1106 24-25 0.31646315980702155 1106 26-27 0.3620922919137328 1106 28-29 0.1729493784670595 1106 30-31 0.46550540400754414 1106 32-33 0.5928151430502098 1106 34-35 0.49433972098265855 1106 36-37 0.5937365332961733 1106 38-39 0.5983840619690355 1106 40-41 0.7828710150138747 1106 42-43 0.4686056304112043 1106 44-45 0.6545407934612584 1106 46-47 0.7397406333989025 1106 48-49 0.7891363701793281 1106 50-51 0.18513285465316898 1106 52-53 0.6747945565941791 1106 54-55 0.7597284323216442 1106 56-57 0.8211582064947862 1106 58-59 0.4141907878852251 1106 60-61 1.3768420887020696 1106 62-63 1.659101054566996 1106 64-65 1.873771238033484 1106 66-67 2.0243588538211803 1106 68-69 1.6064265918319443 1106 70-71 2.03397733559234 1106 72-73 2.4672764584510745 1106 74-75 2.688389978121947 1106 76-77 2.724259875013484 1106 78-79 3.0361848299146814 1106 80-81 3.554389515244605 1106 82-83 3.669585772274221 1106 84-85 3.805486111386408 1106 86-87 4.037855423165958 1106 88-89 4.1038874293995455 1106 90-91 4.528530286549653 1106 92-93 4.730849065661619 1106 94-95 4.743262217398325 1106 96-97 5.110816699573448 1106 98-99 5.245037330233728 1106 100-101 4.846343611540782 1107 1 0.32930121420445957 1107 2 0.2541660194927182 1107 3 0.30819572077948365 1107 4 0.23541953848922503 1107 5 0.22759686444013028 1107 6 0.24349821139602312 1107 7 0.20240890784483412 1107 8 0.20254274011401918 1107 9 0.17873766174469097 1107 10-11 0.27450470456879117 1107 12-13 0.41052430248483773 1107 14-15 0.498665281879461 1107 16-17 0.5833633526423299 1107 18-19 0.6345193265782072 1107 20-21 0.6365427914026398 1107 22-23 0.5829998365970468 1107 24-25 0.6388338776760705 1107 26-27 0.6139078189692455 1107 28-29 0.7273200775849986 1107 30-31 0.7952559179977214 1107 32-33 0.8996276193491681 1107 34-35 0.9630014319278573 1107 36-37 0.9864326791778453 1107 38-39 1.1000326177756747 1107 40-41 1.155958309087815 1107 42-43 1.1443134170324285 1107 44-45 1.2995338271764965 1107 46-47 1.2062412787085393 1107 48-49 1.4863906029344136 1107 50-51 1.516333372381233 1107 52-53 1.4344159124327973 1107 54-55 1.6574346408887806 1107 56-57 1.7984893459480418 1107 58-59 2.0069053517997446 1107 60-61 1.9608255428196983 1107 62-63 2.1956665244439577 1107 64-65 2.290457395987417 1107 66-67 2.436596742268346 1107 68-69 2.6356221962752677 1107 70-71 2.5900030053709564 1107 72-73 2.9760101588620955 1107 74-75 3.090528604104623 1107 76-77 3.2839874707039876 1107 78-79 3.4848899299456946 1107 80-81 3.879171663100575 1107 82-83 4.239509655096548 1107 84-85 4.241670218190766 1107 86-87 4.5585674448244795 1107 88-89 4.684937444782857 1107 90-91 5.027374440878212 1107 92-93 5.1900415885077535 1107 94-95 5.1978773718001605 1107 96-97 5.563409447877028 1107 98-99 5.667801253982827 1107 100-101 5.4152064528326065 1108 1 0.24087739194033375 1108 2 0.19269185155466673 1108 3 0.09073579229293927 1108 4 0.15454350704284536 1108 5 0.05906211415633322 1108 6 0.1166306966958075 1108 7 0.052081894399954365 1108 8 0.002922968250899771 1108 9 0.05809302018749918 1108 10-11 0.0624884725905801 1108 12-13 0.1612190398715896 1108 14-15 0.09163115716473413 1108 16-17 0.3318560222376661 1108 18-19 0.43269886430147153 1108 20-21 0.3733418827644641 1108 22-23 0.3726237621525357 1108 24-25 0.35377649851222515 1108 26-27 0.3161817379731957 1108 28-29 0.4231901479797813 1108 30-31 0.5127259073308323 1108 32-33 0.531439321503079 1108 34-35 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-0.0249061407895681 2311 20-21 0.17459296194850538 2311 22-23 0.03927900785806315 2311 24-25 -0.014718876966391292 2311 26-27 0.14307016486161928 2311 28-29 0.19752240890422712 2311 30-31 0.17065495799730712 2311 32-33 0.24282969769792118 2311 34-35 0.18968183495551472 2311 36-37 0.2808538756573782 2311 38-39 0.5177283850582981 2311 40-41 0.3053198643991877 2311 42-43 0.5664559288811688 2311 44-45 0.6532274857281521 2311 46-47 0.6743627508808245 2311 48-49 0.837531770113138 2311 50-51 0.8313436617781704 2311 52-53 0.936898241511976 2311 54-55 1.0303282433106062 2311 56-57 0.9951528392192159 2311 58-59 0.9905669996094915 2311 60-61 1.0309161937906097 2311 62-63 1.3048283682575388 2311 64-65 1.3323369472834585 2311 66-67 1.4351895055105182 2311 68-69 1.4130152615371436 2311 70-71 1.5873564064076788 2311 72-73 1.519183914817038 2311 74-75 1.7472096958814092 2311 76-77 2.0469719043301353 2311 78-79 2.3687501563574855 2311 80-81 2.3088799231336203 2311 82-83 2.4396518463872816 2311 84-85 2.7257348164215145 2311 86-87 2.8319081548540197 2311 88-89 2.9772952905043404 2311 90-91 3.3372078472704487 2311 92-93 3.5351855531665564 2311 94-95 3.600042490068681 2311 96-97 3.4772887725939157 2311 98-99 3.4256170438461133 2311 100-101 3.2935911880922504 2312 1 -0.43882903907499937 2312 2 -0.3597758684523171 2312 3 -0.29871038249354953 2312 4 -0.3158360261639075 2312 5 -0.3559960600641432 2312 6 -0.23842201437784638 2312 7 -0.2761563029481948 2312 8 -0.2962542942771478 2312 9 -0.33250901962919954 2312 10-11 -0.36340891665759756 2312 12-13 -0.22351257915322975 2312 14-15 -0.09760066426604652 2312 16-17 -0.13213424672643015 2312 18-19 -0.06522926059000156 2312 20-21 -0.13800166261049895 2312 22-23 -0.29301960113480874 2312 24-25 -0.3319672539854537 2312 26-27 -0.16686501339815862 2312 28-29 -0.17389564674999036 2312 30-31 -0.06701957948272508 2312 32-33 -0.03289843262525238 2312 34-35 -0.08224557416659906 2312 36-37 0.10599764927434308 2312 38-39 0.17935931898441027 2312 40-41 0.3720191926431724 2312 42-43 0.3945950268087941 2312 44-45 0.35582585696702296 2312 46-47 0.30196352473039667 2312 48-49 0.5972107395393849 2312 50-51 0.6384814351360788 2312 52-53 0.6670076194934751 2312 54-55 0.8982121630898661 2312 56-57 0.9177306942519579 2312 58-59 0.8526372282683852 2312 60-61 1.2000484265193236 2312 62-63 1.3415690214055118 2312 64-65 1.4899960523423132 2312 66-67 1.60866955827046 2312 68-69 1.6905362965679025 2312 70-71 1.7729861480817632 2312 72-73 1.8463783340746929 2312 74-75 1.8518688106899894 2312 76-77 1.9057660862516386 2312 78-79 1.9738084527463293 2312 80-81 2.4869076652784763 2312 82-83 2.748643390590445 2312 84-85 2.9329355451437884 2312 86-87 3.296957133291805 2312 88-89 3.2828034670412087 2312 90-91 3.4161703790789275 2312 92-93 3.6164596750297555 2312 94-95 3.4352254854347777 2312 96-97 3.6925874136215917 2312 98-99 4.088484312488632 2312 100-101 3.877516051143438 2313 1 -0.17408829082195965 2313 2 -0.2792016847456473 2313 3 -0.14530259289798764 2313 4 -0.054930634634686726 2313 5 -0.05048367674539378 2313 6 -0.011020814006549529 2313 7 0.014579202659973589 2313 8 -0.05790565698450223 2313 9 -0.13769729424688393 2313 10-11 -0.031288420338853484 2313 12-13 0.015108261557422509 2313 14-15 0.16577772876892993 2313 16-17 0.13612972227407738 2313 18-19 0.14659803074301436 2313 20-21 0.2141977973491933 2313 22-23 0.15138329091206515 2313 24-25 0.16012567689529789 2313 26-27 0.23723212343035982 2313 28-29 0.240711711264062 2313 30-31 0.24159310568955306 2313 32-33 0.359662068369893 2313 34-35 0.3528040140420927 2313 36-37 0.4785266549606817 2313 38-39 0.42349909279851516 2313 40-41 0.4686692946930364 2313 42-43 0.5712776524132437 2313 44-45 0.6520270522675204 2313 46-47 0.7179728888096903 2313 48-49 0.6866653748854148 2313 50-51 0.8433995487417363 2313 52-53 0.839598953776175 2313 54-55 0.871713213168249 2313 56-57 1.1325503920603808 2313 58-59 1.1190817656775138 2313 60-61 1.343646469753537 2313 62-63 1.29605710291019 2313 64-65 1.4747582247298254 2313 66-67 1.5477924787138129 2313 68-69 1.9339444664735446 2313 70-71 2.0488773061541288 2313 72-73 2.0772402857415067 2313 74-75 2.1564291511097657 2313 76-77 2.371264637551949 2313 78-79 2.5369328831615583 2313 80-81 2.658011423728052 2313 82-83 2.8059008337516964 2313 84-85 2.9184806633004925 2313 86-87 3.0150010858685476 2313 88-89 3.1080730998447876 2313 90-91 3.436707052943312 2313 92-93 3.7385175187842883 2313 94-95 3.810892683127001 2313 96-97 4.057437958803135 2313 98-99 3.943960214210872 2313 100-101 3.671024932792349 2314 1 -0.39281677968487116 2314 2 -0.3900205690887866 2314 3 -0.3645895595841395 2314 4 -0.2028688124384388 2314 5 -0.32460537295943226 2314 6 -0.24541915156093808 2314 7 -0.2027790673575609 2314 8 -0.18328392830082407 2314 9 -0.2980583566535344 2314 10-11 -0.24674396940048382 2314 12-13 -0.1615655484302394 2314 14-15 -0.1265529420538556 2314 16-17 -0.2747151377817758 2314 18-19 -0.19133949766792568 2314 20-21 -0.10917060319629712 2314 22-23 -0.1945937205821906 2314 24-25 -0.16317453740432342 2314 26-27 -0.09620186578971612 2314 28-29 -0.1396733786768536 2314 30-31 -0.02078107445201738 2314 32-33 0.12730282426313266 2314 34-35 0.08191304709618663 2314 36-37 0.1688052444794792 2314 38-39 0.17885983050874898 2314 40-41 0.2619221033169765 2314 42-43 0.4186605993578496 2314 44-45 0.4144466899063275 2314 46-47 0.4083521342152281 2314 48-49 0.5202422305253904 2314 50-51 0.5036696961539135 2314 52-53 0.5743219877394239 2314 54-55 0.6999423189543421 2314 56-57 0.753477075611265 2314 58-59 1.0614546470334503 2314 60-61 1.0987312155162456 2314 62-63 1.2379819032219004 2314 64-65 1.262536651900426 2314 66-67 1.4523934268273244 2314 68-69 1.8287882875008883 2314 70-71 1.8804962424486895 2314 72-73 1.9543751646297487 2314 74-75 2.1479545748385753 2314 76-77 2.299938577418171 2314 78-79 2.375958144542164 2314 80-81 2.6314317756989567 2314 82-83 2.728340602567851 2314 84-85 2.9771143408120224 2314 86-87 3.1626665186888587 2314 88-89 3.3653365585109363 2314 90-91 3.5958959613139854 2314 92-93 3.667509661131195 2314 94-95 3.868536036591511 2314 96-97 3.998836750932494 2314 98-99 4.111441868863771 2314 100-101 3.7516243223630994 2315 1 -0.5128709875966884 2315 2 -0.392969975037758 2315 3 -0.409492384197069 2315 4 -0.3796512676882031 2315 5 -0.28500398543027217 2315 6 -0.24393160668984137 2315 7 -0.4001272912477205 2315 8 -0.4059887036733869 2315 9 -0.28467015065137957 2315 10-11 -0.3117812260087689 2315 12-13 -0.2660310535851309 2315 14-15 -0.38470407153642583 2315 16-17 -0.2947352013567155 2315 18-19 -0.3075731657970593 2315 20-21 -0.2601405440933533 2315 22-23 -0.3619579288566044 2315 24-25 -0.46879838163363274 2315 26-27 -0.32343877309842384 2315 28-29 -0.32933127055110134 2315 30-31 -0.25165344715104965 2315 32-33 -0.1280391025652463 2315 34-35 -0.09175456836001672 2315 36-37 0.11942331740937107 2315 38-39 0.006955029754557529 2315 40-41 0.03061189748403592 2315 42-43 0.24580595149775775 2315 44-45 0.2697839332194718 2315 46-47 0.34167942430310205 2315 48-49 0.4443339307320642 2315 50-51 0.5079702207076124 2315 52-53 0.6798384132805921 2315 54-55 0.6697037316234002 2315 56-57 1.0002433349631872 2315 58-59 1.1133692982777532 2315 60-61 1.1284455513031375 2315 62-63 1.2745527542317276 2315 64-65 1.3340212960137023 2315 66-67 1.5319583433701034 2315 68-69 1.7316665342345097 2315 70-71 1.8059797020858532 2315 72-73 1.9947991889477699 2315 74-75 2.1903704528617105 2315 76-77 2.350955655629857 2315 78-79 2.519791767951279 2315 80-81 2.77200657899132 2315 82-83 3.039747379463318 2315 84-85 3.273471321364859 2315 86-87 3.411385652812651 2315 88-89 3.8525785119295968 2315 90-91 3.904411951741487 2315 92-93 3.9190657968383142 2315 94-95 4.0736989322730075 2315 96-97 4.233349725780506 2315 98-99 4.343098201580691 2315 100-101 3.8112226580533033 2316 1 -0.653141386351443 2316 2 -0.48187925328349834 2316 3 -0.4528843269948375 2316 4 -0.1861267030635645 2316 5 -0.18238003233032174 2316 6 -0.18589509841159213 2316 7 -0.3349597270630369 2316 8 -0.27367728609416275 2316 9 -0.3872620097372632 2316 10-11 -0.25085734298075835 2316 12-13 -0.3536508043751141 2316 14-15 -0.7183490956938599 2316 16-17 -0.42786919520268185 2316 18-19 -0.3585078705254361 2316 20-21 -0.192212560386416 2316 22-23 -0.42251807972521505 2316 24-25 -0.556513327949709 2316 26-27 -0.26696233478512 2316 28-29 -0.3051957361924522 2316 30-31 -0.11864707715788825 2316 32-33 -0.13924008063033 2316 34-35 -0.1344789723808617 2316 36-37 0.019962546992566388 2316 38-39 0.07954441826998249 2316 40-41 -0.021459386664325564 2316 42-43 0.07222597789508711 2316 44-45 0.09961704100321356 2316 46-47 0.2791512584626332 2316 48-49 0.3372577689621892 2316 50-51 0.5615786744046432 2316 52-53 0.6835500358603355 2316 54-55 0.6091460045214774 2316 56-57 0.9061420141607783 2316 58-59 1.231917649247194 2316 60-61 1.0975324966617492 2316 62-63 1.2937955394304979 2316 64-65 1.442099392785181 2316 66-67 1.6960945697559318 2316 68-69 2.014242543557824 2316 70-71 1.8165521723754914 2316 72-73 2.1433400646864307 2316 74-75 2.3263211143327176 2316 76-77 2.451650290446782 2316 78-79 2.605265565863295 2316 80-81 2.8872858537468353 2316 82-83 3.2299653412794633 2316 84-85 3.3618421055152297 2316 86-87 3.889650483255963 2316 88-89 3.966882931007884 2316 90-91 4.157972966473977 2316 92-93 4.2704394206403045 2316 94-95 4.315178389429505 2316 96-97 4.169241932685267 2316 98-99 4.023802210524227 2316 100-101 3.8315624902016054 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5993.0 3 1740.0 4 1821.0 5 2135.0 6 2430.0 7 3041.0 8 3893.0 9 4948.0 10 6515.0 11 8642.0 12 11063.0 13 14112.0 14 17148.0 15 20411.0 16 24816.0 17 29419.0 18 28936.0 19 27837.0 20 30354.0 21 33519.0 22 37905.0 23 42984.0 24 49179.0 25 55867.0 26 64061.0 27 72344.0 28 82251.0 29 93682.0 30 107727.0 31 125531.0 32 148055.0 33 175300.0 34 212139.0 35 256503.0 36 303165.0 37 315332.0 38 177436.0 39 13723.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.80984012994457 18.464928057899197 14.550275011200464 24.174956800955773 2 15.591260924897066 19.356269314007804 40.27987191717893 24.7725978439162 3 19.436358733869646 23.163403188765937 29.137092116330056 28.263145961034365 4 13.110395895309873 16.024102338418615 36.26331789401758 34.602183872253924 5 14.35005784243873 36.5806308273384 34.01261807901814 15.056693251204731 6 33.425422089426455 35.159747560356635 17.057756581530533 14.357073768686377 7 29.411323171002586 31.640594482562122 20.27801649195852 18.67006585447677 8 26.3753851338801 35.08625658485583 19.37781130977115 19.160546971492913 9 26.192955652930923 16.854329784337104 19.82333788974455 37.129376672987426 10-11 25.200895532791073 26.09861124249047 27.230665181260754 21.4698280434577 12-13 25.88880563418795 23.234744490939843 27.585752607922124 23.29069726695008 14-15 22.80455017967232 24.91332320931313 25.645856258699034 26.63627035231552 16-17 22.69102487516466 28.051975155469776 26.295721900560608 22.96127806880495 18-19 22.588621574922797 26.761651542816526 28.398538980932308 22.251187901328368 20-21 23.06826300841647 25.98538918729389 28.603991211834956 22.34235659245469 22-23 22.683804810365913 25.848933439313782 28.49908288361536 22.96817886670495 24-25 22.56341298097079 26.095490979334794 28.600274759738447 22.740821279955963 26-27 22.354702163349625 26.460916438435127 28.389379568341244 22.795001829874 28-29 22.387769600943688 26.63742538878261 28.44544404804991 22.529360962223794 30-31 22.53300232705279 26.27365543379849 28.439603098856082 22.753739140292637 32-33 22.319789302595268 26.323197012183957 28.427509384796974 22.9295043004238 34-35 22.86182056319581 26.430297529459974 28.196593864053277 22.51128804329094 36-37 22.876296704085785 26.15599224490773 28.198606955647577 22.769104095358912 38-39 22.407686160324015 26.46077980500854 28.20330392621249 22.928230108454954 40-41 22.774635798322 26.33634431099834 28.197784452955094 22.691235437724572 42-43 22.557924252127854 26.301051984640967 28.125028120892555 23.015995642338623 44-45 22.48446194240796 26.542783382295088 28.19501997237466 22.777734702922295 46-47 22.64914072023614 26.68030113514417 27.685922960147515 22.984635184472175 48-49 22.689000817896932 26.634951462258805 27.767371119967404 22.908676599876856 50-51 22.751995423825473 26.901661447701454 27.567814162593944 22.778528965879126 52-53 22.790007971385712 26.7855789132727 27.5424393971764 22.881973718165188 54-55 22.594018104389793 26.793252539678136 27.688421479842905 22.924307876089166 56-57 22.632047765751935 26.935257450763917 27.589549777272982 22.843145006211163 58-59 22.6948749771947 26.658660643196498 27.63276512007982 23.013699259528977 60-61 22.649970987591573 26.83725311933643 27.709616325974267 22.803159567097726 62-63 22.4521816537208 26.88164941348763 27.815608742886955 22.850560189904616 64-65 22.368987820861097 26.74174627770305 27.797719622890455 23.091546278545394 66-67 22.466008422231045 26.824315813372422 27.576853145434143 23.132822618962386 68-69 22.554091097834057 26.678375181318298 27.763556922697575 23.00397679815007 70-71 22.53253661643695 26.652944736332746 27.645943565227064 23.168575082003244 72-73 22.592550969634466 26.691640866991612 27.531327675433165 23.18448048794076 74-75 22.465681199285672 27.020876919448266 27.337568652917195 23.17587322834887 76-77 22.575611410923543 26.81476288377167 27.371961563341934 23.237664141962853 78-79 22.67167802441266 26.81200191209091 27.4151873391555 23.10113272434093 80-81 22.666845090138313 26.930400296504587 27.221142492200674 23.181612121156427 82-83 22.7233784461364 26.906997273933587 27.220995555089267 23.148628724840748 84-85 22.783846165774037 26.773239892558937 27.213177017597296 23.229736924069737 86-87 22.729808298623965 26.703655959756915 27.27756173890005 23.288974002719065 88-89 22.755532478035512 26.801214480020587 27.171444019654693 23.271809022289208 90-91 22.81191975703539 26.697174469874422 27.1505981151899 23.340307657900286 92-93 22.81661298987333 26.390239158162043 27.236648990530394 23.556498861434232 94-95 22.717947412045305 26.521803979489285 27.243290085396215 23.516958523069192 96-97 22.820368341959558 26.413629491097783 27.176845917128134 23.589156249814526 98-99 22.816892348267988 26.381524434417553 26.984458831295537 23.81712438601892 100-101 23.29839375536879 25.94251687977682 27.103392549157196 23.65569681569719 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 478.0 1 330.0 2 275.0 3 399.0 4 368.5 5 367.0 6 659.0 7 1528.5 8 2536.5 9 2813.5 10 2388.0 11 2024.0 12 2043.5 13 2153.5 14 2318.5 15 2491.0 16 2621.0 17 2759.5 18 2985.0 19 3346.5 20 3734.5 21 4346.5 22 5291.5 23 6536.0 24 8182.5 25 10403.5 26 13623.0 27 17313.0 28 20288.5 29 23422.5 30 27350.5 31 32331.0 32 37846.0 33 41410.0 34 44626.0 35 49109.0 36 54749.5 37 60313.5 38 64298.5 39 67640.0 40 71457.0 41 77139.5 42 82061.5 43 86032.0 44 90945.5 45 95106.0 46 97413.0 47 99233.5 48 99822.0 49 99570.0 50 99434.5 51 96797.0 52 94251.0 53 93936.5 54 93570.0 55 87783.0 56 78544.0 57 71297.0 58 65451.0 59 58140.5 60 48331.5 61 39935.5 62 33156.0 63 26313.5 64 21323.0 65 17871.5 66 14082.0 67 10962.5 68 8310.0 69 6074.5 70 4803.5 71 3690.5 72 2824.0 73 2329.5 74 1849.5 75 1443.5 76 1094.5 77 748.5 78 477.0 79 290.5 80 184.5 81 134.5 82 94.0 83 56.0 84 34.0 85 26.0 86 17.5 87 12.5 88 11.0 89 8.0 90 5.5 91 3.0 92 3.0 93 3.5 94 4.0 95 2.5 96 0.5 97 0.5 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2739706097990053 2 0.03200662797540084 3 0.03993171408892712 4 0.041807700656863296 5 0.05432703958982511 6 0.05490132119225455 7 0.028599223800986156 8 0.032695765898316166 9 0.03774944399969525 10-11 0.029709501565683076 12-13 0.031719487174186115 14-15 0.025938385709729745 16-17 0.019870143444058654 18-19 0.02415811274219848 20-21 0.036275454553459686 22-23 0.030073213247221724 24-25 0.0503262110929 26-27 0.04301369202196512 28-29 0.03537574670965356 30-31 0.0356437447907873 32-33 0.031432346372971404 34-35 0.03020721228778859 36-37 0.029537217084954243 38-39 0.017611302474502856 40-41 0.017592159754421872 42-43 0.017860157835555612 44-45 0.008671652196684558 46-47 0.01686473639134458 48-49 0.014663323582031724 50-51 0.006527667547614644 52-53 0.004479396498949639 54-55 0.0061830985861569794 56-57 0.004957964500974173 58-59 0.006719094748424459 60-61 0.005570531543565577 62-63 0.004651680979678471 64-65 0.00758051715206862 66-67 0.002373697290041689 68-69 0.007063663709882123 70-71 0.012423625332556905 72-73 0.01265333797352868 74-75 0.0103753542838919 76-77 0.008939650277818297 78-79 0.006068242265671092 80-81 0.007465660831582731 82-83 0.012270483571909054 84-85 0.004153970257572956 86-87 0.001225134085182807 88-89 0.018032442316284442 90-91 0.027354946995722367 92-93 0.013017049655067326 94-95 0.02080813672802674 96-97 0.017056163592154394 98-99 0.012059913651018259 100-101 0.09760872969292397 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2611959.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.722686005670056 #Duplication Level Percentage of deduplicated Percentage of total 1 76.70517655216868 42.74218468025981 2 13.231481533048669 14.745873817117857 3 4.286211722377164 7.165176898795349 4 1.9317829054373445 4.305765290832246 5 1.023341226158903 2.8511660910954966 6 0.6180805058729696 2.0664663572991118 7 0.40788021210540637 1.5909726690952963 8 0.297725257969063 1.3272040852613773 9 0.20873875239318745 1.0468335561138804 >10 1.1457729645233838 11.680606461360746 >50 0.09169894107985165 3.517318643066314 >100 0.04866615292385069 4.8311518873180885 >500 0.0024102917591253257 0.8805227948183545 >1k 8.95251224801945E-4 0.7146716870245723 >5k 1.3773095766183769E-4 0.5340850805416665 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8050 0.3081977933037999 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5868 0.22465896287039727 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4385 0.16788165511020656 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 3.828544016196273E-5 0.0 0.0 0.0 0.0 7 3.828544016196273E-5 0.0 0.0 0.0 0.0 8 3.828544016196273E-5 0.0 0.0 7.657088032392546E-5 0.0 9 3.828544016196273E-5 0.0 0.0 1.9142720080981364E-4 0.0 10-11 1.5314176064785092E-4 0.0 0.0 1.9142720080981364E-4 0.0 12-13 2.2971264097177636E-4 0.0 0.0 2.2971264097177636E-4 0.0 14-15 2.488553610527577E-4 0.0 0.0 2.8714080121472046E-4 0.0 16-17 2.679980811337391E-4 0.0 0.0 3.445689614576645E-4 0.0 18-19 2.679980811337391E-4 0.0 0.0 3.637116815386459E-4 0.0 20-21 2.679980811337391E-4 0.0 0.0 3.828544016196273E-4 0.0 22-23 2.679980811337391E-4 0.0 0.0 4.2113984178158997E-4 0.0 24-25 3.2542624137668315E-4 0.0 0.0 5.551388823484595E-4 0.0 26-27 3.828544016196273E-4 0.0 0.0 8.4227968356318E-4 0.0 28-29 3.828544016196273E-4 0.0 0.0 0.0021822700892318754 0.0 30-31 4.2113984178158997E-4 0.0 0.0 0.005436532502998707 0.0 32-33 4.2113984178158997E-4 0.0 0.0 0.014644180861950743 0.0 34-35 4.5942528194355267E-4 0.0 0.0 0.02988178604641191 0.0 36-37 4.977107221055154E-4 0.0 0.0 0.05796415640521157 0.0 38-39 4.977107221055154E-4 0.0 0.0 0.09917843273956445 0.0 40-41 4.977107221055154E-4 0.0 0.0 0.14594409789740193 0.0 42-43 4.977107221055154E-4 0.0 0.0 0.19604059634933013 0.0 44-45 4.977107221055154E-4 0.0 0.0 0.2586372910141392 0.0 46-47 4.977107221055154E-4 0.0 0.0 0.3340404654131248 0.0 48-49 4.977107221055154E-4 0.0 0.0 0.4154735966376195 0.0 50-51 4.977107221055154E-4 0.0 0.0 0.5048509566957216 0.0 52-53 5.359961622674782E-4 0.0 0.0 0.5913951941818383 0.0 54-55 5.742816024294409E-4 0.0 0.0 0.6918179037266665 0.0 56-57 5.742816024294409E-4 0.0 0.0 0.8119767576749866 0.0 58-59 5.742816024294409E-4 0.0 0.0 0.9431618183899517 0.0 60-61 5.742816024294409E-4 0.0 0.0 1.0856219412326151 0.0 62-63 5.742816024294409E-4 0.0 0.0 1.2307046167263729 0.0 64-65 5.742816024294409E-4 0.0 0.0 1.387808920430987 0.0 66-67 5.742816024294409E-4 0.0 0.0 1.5652236501415222 0.0 68-69 5.742816024294409E-4 0.0 0.0 1.7664710663528793 0.0 70-71 5.742816024294409E-4 0.0 0.0 1.987531197848052 0.0 72-73 5.742816024294409E-4 0.0 0.0 2.2111181683939147 0.0 74-75 5.742816024294409E-4 0.0 0.0 2.4443913553007532 0.0 76-77 5.742816024294409E-4 0.0 0.0 2.7003486655035553 0.0 78-79 5.742816024294409E-4 0.0 0.0 2.990935156332852 0.0 80-81 5.742816024294409E-4 0.0 0.0 3.3005303682025637 0.0 82-83 5.742816024294409E-4 0.0 0.0 3.617725239944425 0.0 84-85 5.742816024294409E-4 0.0 0.0 3.9388635120229685 0.0 86-87 5.742816024294409E-4 0.0 0.0 4.281537344192616 0.0 88-89 5.742816024294409E-4 0.0 0.0 4.6503984174330455 1.9142720080981364E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3610 0.0 50.659866 1 GTATCAA 6360 0.0 45.004646 1 ATCAACG 8245 0.0 34.122944 3 TATCAAC 8530 0.0 33.261414 2 TCAACGC 8600 0.0 32.714375 4 CAACGCA 8780 0.0 32.043697 5 AACGCAG 9085 0.0 30.912067 6 ACGCAGA 10645 0.0 26.244537 7 CGCAGAG 10645 0.0 26.110636 8 GTACATG 7340 0.0 24.202085 1 TACATGG 7070 0.0 23.594082 2 GCAGAGT 11790 0.0 23.252035 9 GTGGTAT 2105 0.0 22.851215 1 TGGTATC 1895 0.0 22.320105 2 TAGCGCG 175 4.034602E-6 21.725378 4 ACATGGG 7535 0.0 21.696545 3 GAGTACT 7300 0.0 21.441162 12-13 GGACCGA 795 0.0 20.322506 6 AGTACTT 7720 0.0 20.213142 12-13 GTACTTT 7785 0.0 19.771118 14-15 >>END_MODULE