FastQCFastQC Report
Thu 26 May 2016
SRR938293_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938293_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2611959
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78200.2993921420665485No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64420.2466348055233639No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49550.1897043560025253No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA42700.050.7823221
GTATCAA72950.042.82681
ATCAACG94450.032.663313
TATCAAC96000.032.3457762
TCAACGC97350.031.6902894
CAACGCA100400.030.7749335
AACGCAG104200.029.6282166
ACGCAGA121000.025.475297
CGCAGAG121050.025.3862958
TGGTATC21300.023.6647472
GCAGAGT129850.023.0074529
GAGTACT82900.021.97205512-13
GTGGTAT24650.021.7990261
GGACCGA9700.021.0550616
AGAGTAC119650.020.54269810-11
GTACATG74600.020.4617041
TACATGG71900.020.3702982
GTACTTT88650.020.33259814-15
CAGAGTA124750.020.06457310-11
AGTACTT89550.020.0486912-13
Produced by FastQC (version 0.11.5)