##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938293_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2611959 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.427199278396024 31.0 31.0 34.0 30.0 34.0 2 31.693399475259756 31.0 31.0 34.0 30.0 34.0 3 31.75224266537109 31.0 31.0 34.0 30.0 34.0 4 35.49199394018053 37.0 35.0 37.0 33.0 37.0 5 35.34992164884671 37.0 35.0 37.0 33.0 37.0 6 35.40589993947072 37.0 35.0 37.0 33.0 37.0 7 35.32813417055934 37.0 35.0 37.0 32.0 37.0 8 35.30686507713176 37.0 35.0 37.0 32.0 37.0 9 36.810252764304494 39.0 37.0 39.0 32.0 39.0 10-11 36.71590939980298 39.0 35.0 39.0 32.0 39.0 12-13 36.58299039150308 38.0 35.0 39.0 32.0 39.0 14-15 37.48875097197161 39.0 36.0 40.5 32.0 41.0 16-17 37.359260041983816 39.0 36.0 40.0 32.0 41.0 18-19 37.33407071091085 39.0 36.0 40.0 32.0 41.0 20-21 37.313231945830694 39.0 36.0 40.0 31.5 41.0 22-23 37.338048376716486 39.0 36.0 40.0 32.0 41.0 24-25 37.234132695038475 39.0 36.0 40.0 31.0 41.0 26-27 36.996594510097594 39.0 36.0 40.0 31.0 41.0 28-29 36.81823757570467 39.0 36.0 40.0 30.5 41.0 30-31 36.5160733763432 38.0 35.0 40.0 30.0 41.0 32-33 36.25335485740779 38.0 35.0 40.0 30.0 41.0 34-35 36.02933392139769 38.0 35.0 40.0 29.5 41.0 36-37 36.00728514498122 38.0 35.0 40.0 29.5 41.0 38-39 35.8097096853358 38.0 35.0 40.0 29.0 41.0 40-41 35.575404705816595 38.0 34.0 40.0 27.5 41.0 42-43 35.28987265879748 38.0 34.0 40.0 27.0 41.0 44-45 34.933170658498085 38.0 33.0 40.0 26.0 41.0 46-47 34.481444769998305 38.0 33.0 40.0 24.5 41.0 48-49 33.987977414653145 37.0 33.0 40.0 24.0 41.0 50-51 33.508416479738 37.0 32.0 40.0 23.0 41.0 52-53 32.836231541153595 36.5 31.0 39.0 20.5 40.0 54-55 32.37520439639366 36.0 30.5 39.0 18.5 40.0 56-57 31.87955036813365 35.5 30.0 39.0 15.5 40.0 58-59 31.27689351172817 35.0 29.0 38.5 11.5 40.0 60-61 30.71898908060961 34.5 28.0 38.0 8.0 40.0 62-63 30.08343967114338 34.0 28.0 38.0 7.5 40.0 64-65 29.43092349458778 34.0 26.5 37.5 4.0 39.5 66-67 28.787175066683666 33.5 26.0 37.0 2.0 39.0 68-69 28.065610333087157 32.5 25.5 36.0 2.0 39.0 70-71 27.353574845546966 32.0 24.0 36.0 2.0 38.5 72-73 26.619902150072036 31.5 22.0 35.0 2.0 38.0 74-75 25.855076974791718 31.0 20.5 35.0 2.0 37.0 76-77 24.7940622345144 29.5 20.0 34.0 2.0 36.0 78-79 25.291370768071015 31.0 20.0 34.0 2.0 36.0 80-81 24.833570703062335 31.0 18.0 34.0 2.0 36.0 82-83 24.173878877884377 30.5 13.5 34.0 2.0 35.5 84-85 23.39538714045665 30.0 7.0 34.0 2.0 35.0 86-87 22.548479130032284 29.0 2.0 34.0 2.0 35.0 88-89 21.60345797924087 29.0 2.0 34.0 2.0 35.0 90-91 20.86359491094615 28.0 2.0 33.5 2.0 35.0 92-93 19.879883642890263 26.5 2.0 33.0 2.0 35.0 94-95 18.88652080679674 25.0 2.0 33.0 2.0 34.0 96-97 17.7296469814419 23.5 2.0 32.0 2.0 34.0 98-99 16.450146039811496 10.0 2.0 32.0 2.0 34.0 100-101 13.885483079941148 2.0 2.0 29.5 2.0 33.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.35848357092644534 1101 2 0.3099822212292125 1101 3 0.41771860572982433 1101 4 0.30788716882889844 1101 5 0.2930804005250778 1101 6 0.26866382086814156 1101 7 0.24529173937781223 1101 8 0.33757554725176675 1101 9 0.3638486958277838 1101 10-11 0.35917817973967203 1101 12-13 0.5095502997213472 1101 14-15 0.6969013396636825 1101 16-17 0.7342145765017065 1101 18-19 0.730226694766202 1101 20-21 0.7323765947522674 1101 22-23 0.7422401916967019 1101 24-25 0.6888467933146245 1101 26-27 0.7045117317379592 1101 28-29 0.7432577956937436 1101 30-31 0.7055276727557782 1101 32-33 0.7286365058393258 1101 34-35 0.6633339721129587 1101 36-37 0.8686918744418293 1101 38-39 0.9767158424530322 1101 40-41 1.1684535802313292 1101 42-43 1.0472666087472433 1101 44-45 1.2392548730493687 1101 46-47 1.291153613099901 1101 48-49 1.4105076478583598 1101 50-51 1.5888896592803263 1101 52-53 1.6273895820727518 1101 54-55 1.7186602306412553 1101 56-57 1.8167132447858272 1101 58-59 1.8744399537198824 1101 60-61 2.0624663043641505 1101 62-63 2.0355295354337777 1101 64-65 2.1496055425856504 1101 66-67 2.2848394625407664 1101 68-69 2.5495965973710994 1101 70-71 2.693850048016291 1101 72-73 2.7046847793369544 1101 74-75 2.752164678037005 1101 76-77 2.7096088359986155 1101 78-79 3.2335177498558956 1101 80-81 3.46766821926623 1101 82-83 3.77721752927733 1101 84-85 4.004137885077263 1101 86-87 4.182771607080042 1101 88-89 4.373798057246091 1101 90-91 4.738434854841351 1101 92-93 5.094832501184303 1101 94-95 4.986890891620671 1101 96-97 5.167538421438373 1101 98-99 5.227131068168223 1101 100-101 4.958282320681921 1102 1 0.3610697142039143 1102 2 0.37670535951087203 1102 3 0.35132996550856177 1102 4 0.23785915762442045 1102 5 0.24721138472478543 1102 6 0.23132383714651183 1102 7 0.30003444592969686 1102 8 0.2596107607262397 1102 9 0.3362719912245993 1102 10-11 0.3543038932553131 1102 12-13 0.47511304257336207 1102 14-15 0.6597479903514483 1102 16-17 0.6117477776401259 1102 18-19 0.6192919237596684 1102 20-21 0.6888530761428413 1102 22-23 0.5975286033576594 1102 24-25 0.5693161440942589 1102 26-27 0.5593691195193458 1102 28-29 0.6279530254465868 1102 30-31 0.6759244607187682 1102 32-33 0.5793053147909362 1102 34-35 0.709096167789248 1102 36-37 0.9439207823470994 1102 38-39 1.0731464727425717 1102 40-41 1.0440777894415447 1102 42-43 1.0774068626342128 1102 44-45 1.117943041711257 1102 46-47 1.245676315651714 1102 48-49 1.334543833543016 1102 50-51 1.4088957449524742 1102 52-53 1.6939010854904666 1102 54-55 1.3487519007571862 1102 56-57 1.8017235337429476 1102 58-59 2.058391827711173 1102 60-61 2.091249715399428 1102 62-63 2.1954135921492473 1102 64-65 2.3623830743696494 1102 66-67 2.375448863995125 1102 68-69 2.5520174970024314 1102 70-71 2.7261935505184773 1102 72-73 2.7458595921410343 1102 74-75 2.8116711075656724 1102 76-77 2.86609737643197 1102 78-79 3.3816026615601764 1102 80-81 3.592641079735351 1102 82-83 3.5235274889236585 1102 84-85 3.792838989803233 1102 86-87 3.9915333706629106 1102 88-89 4.092519146292631 1102 90-91 4.197077151011442 1102 92-93 4.51443371318495 1102 94-95 4.640642884972422 1102 96-97 5.18943881962743 1102 98-99 5.23717697274617 1102 100-101 5.296768688883269 1103 1 0.23428746676179912 1103 2 0.309448928125839 1103 3 0.3000006149208261 1103 4 0.292230189704874 1103 5 0.1997256229362563 1103 6 0.2523068615777646 1103 7 0.21646753726025025 1103 8 0.27044444260183553 1103 9 0.3234144232796652 1103 10-11 0.4023918008156713 1103 12-13 0.4385687766657824 1103 14-15 0.6320228840720716 1103 16-17 0.6315830836186151 1103 18-19 0.5636691307604735 1103 20-21 0.6771375043150414 1103 22-23 0.6136329465063639 1103 24-25 0.6081251543798984 1103 26-27 0.5661000168023875 1103 28-29 0.5890319094038645 1103 30-31 0.7076571527343418 1103 32-33 0.8110089269372693 1103 34-35 0.911205810861162 1103 36-37 1.0622308517850456 1103 38-39 1.2582845787119012 1103 40-41 1.125093316739168 1103 42-43 1.1763900035930277 1103 44-45 1.2012227931746153 1103 46-47 1.392160434609707 1103 48-49 1.4776965127405575 1103 50-51 1.587669573278248 1103 52-53 1.9058084075830521 1103 54-55 1.9518425082195279 1103 56-57 1.9866404194190075 1103 58-59 2.244857905705569 1103 60-61 2.27057483874178 1103 62-63 2.4028796014756075 1103 64-65 2.3388887249159787 1103 66-67 2.477739384894136 1103 68-69 2.717696280454689 1103 70-71 2.8128142356173065 1103 72-73 3.004753142132401 1103 74-75 3.1236766457461336 1103 76-77 2.8682545669108386 1103 78-79 3.236258327816916 1103 80-81 3.445050209045572 1103 82-83 3.8292143980924997 1103 84-85 3.9719622707615905 1103 86-87 4.084512220910426 1103 88-89 4.4421059936602525 1103 90-91 4.656487063761428 1103 92-93 4.788121711678411 1103 94-95 4.69565377821861 1103 96-97 5.129797516433118 1103 98-99 5.205043977342765 1103 100-101 4.986535514110656 1104 1 0.33662931483359415 1104 2 0.2980959575589317 1104 3 0.3072478858790433 1104 4 0.25143514926357113 1104 5 0.1618375063164379 1104 6 0.27661879815820356 1104 7 0.2536247824578197 1104 8 0.2849750160621838 1104 9 0.28184792851928364 1104 10-11 0.35691088969549867 1104 12-13 0.4067459067242325 1104 14-15 0.5597956314810872 1104 16-17 0.6089056516537568 1104 18-19 0.5595160844388261 1104 20-21 0.6230860795898678 1104 22-23 0.5700665746325058 1104 24-25 0.5862131082635642 1104 26-27 0.7340920332236678 1104 28-29 0.7036422453240547 1104 30-31 0.7790624436315383 1104 32-33 0.7266468192765316 1104 34-35 0.7609606572934595 1104 36-37 0.81556433851582 1104 38-39 0.8289803642602465 1104 40-41 0.967879346513115 1104 42-43 0.9786507870123486 1104 44-45 1.1527104281601552 1104 46-47 1.2218194143784444 1104 48-49 1.3546675646189001 1104 50-51 1.5738156342845855 1104 52-53 1.7427471499127591 1104 54-55 1.7946432666772054 1104 56-57 1.9475773584015492 1104 58-59 2.166710607236947 1104 60-61 2.0663912224997176 1104 62-63 2.186518081950812 1104 64-65 2.3453089139083794 1104 66-67 2.7266249987755593 1104 68-69 2.7950455118323205 1104 70-71 2.902585057298335 1104 72-73 2.95565795109189 1104 74-75 3.2077078321220114 1104 76-77 3.0882192408266285 1104 78-79 3.4640584086287127 1104 80-81 3.9896832410456256 1104 82-83 4.030282929330461 1104 84-85 4.152838282299772 1104 86-87 4.316023487531716 1104 88-89 4.3905078113694636 1104 90-91 4.776271845453259 1104 92-93 5.006067228444081 1104 94-95 5.070344918352742 1104 96-97 5.350905528129083 1104 98-99 5.480289067697235 1104 100-101 5.235247418022489 1105 1 0.3270147033106703 1105 2 0.2961838453496526 1105 3 0.2169617737874283 1105 4 0.22807566089239373 1105 5 0.2195879279346613 1105 6 0.31061532225907484 1105 7 0.22943713293042123 1105 8 0.2395376851997284 1105 9 0.3754241335630155 1105 10-11 0.36497587037950296 1105 12-13 0.4957455316801145 1105 14-15 0.5440821406782419 1105 16-17 0.6084117209952495 1105 18-19 0.6366927498308357 1105 20-21 0.5783592489823448 1105 22-23 0.41262709645859985 1105 24-25 0.45289344075366955 1105 26-27 0.6614513181395338 1105 28-29 0.6817894905881943 1105 30-31 0.6454630863542121 1105 32-33 0.8157954200923072 1105 34-35 0.9121440072954172 1105 36-37 0.8405722438576291 1105 38-39 0.8771678961267355 1105 40-41 0.8635369851568697 1105 42-43 0.9751816901578181 1105 44-45 1.0722481433815503 1105 46-47 1.1586052372203426 1105 48-49 1.2828531109576886 1105 50-51 1.276669891648197 1105 52-53 1.482198788831063 1105 54-55 1.4366672057680816 1105 56-57 1.5561943035323544 1105 58-59 1.8272813974279494 1105 60-61 1.833931950330438 1105 62-63 2.0289103241753885 1105 64-65 2.303527772989842 1105 66-67 2.270258686072104 1105 68-69 2.232093068897857 1105 70-71 2.4946462556746134 1105 72-73 2.525471066360357 1105 74-75 2.819237374813312 1105 76-77 2.699594108458111 1105 78-79 3.1159413507395506 1105 80-81 3.570644665428887 1105 82-83 3.6081328356919933 1105 84-85 3.687613635772731 1105 86-87 4.117969917503871 1105 88-89 4.215635522472731 1105 90-91 4.438768781367287 1105 92-93 4.668429038166796 1105 94-95 4.6421815559963875 1105 96-97 4.870283727034838 1105 98-99 4.936316860182625 1105 100-101 4.51072566056083 1106 1 0.25271806995354495 1106 2 0.240285528671091 1106 3 0.1474816405492625 1106 4 0.2109666018869163 1106 5 0.025848337166166857 1106 6 0.06016846882105398 1106 7 0.14284124903796425 1106 8 0.10286075800487282 1106 9 0.24127293204029598 1106 10-11 0.36850943271593195 1106 12-13 0.24672808878218433 1106 14-15 0.1979105073211187 1106 16-17 0.26681791894909423 1106 18-19 0.445681492990488 1106 20-21 0.5677358847187222 1106 22-23 0.4067272927469716 1106 24-25 0.3100561073013637 1106 26-27 0.4155343391959221 1106 28-29 0.5143588606809928 1106 30-31 0.5552652366040292 1106 32-33 0.4964602725786307 1106 34-35 0.4866228022038186 1106 36-37 0.7075054612023806 1106 38-39 0.7348023239164121 1106 40-41 0.5183196552734586 1106 42-43 0.8110024580616937 1106 44-45 0.4465975664127271 1106 46-47 0.352791681426865 1106 48-49 0.9187106829300902 1106 50-51 0.8372040339275202 1106 52-53 1.1747885312769313 1106 54-55 1.160613642836843 1106 56-57 1.02979203134581 1106 58-59 1.6127274329977759 1106 60-61 1.5946724184484538 1106 62-63 1.796748772902486 1106 64-65 1.5050103449622405 1106 66-67 1.713267400085904 1106 68-69 1.7643903119385413 1106 70-71 2.09233763683093 1106 72-73 2.405800592889026 1106 74-75 2.688269911096917 1106 76-77 2.784943858636556 1106 78-79 3.154871133037908 1106 80-81 3.0819293876511082 1106 82-83 3.369323131314168 1106 84-85 3.5676275395319514 1106 86-87 3.962584627235014 1106 88-89 4.320300345218389 1106 90-91 4.041194875823631 1106 92-93 3.8227432928658196 1106 94-95 4.261321158661151 1106 96-97 4.7685093248221335 1106 98-99 4.716004731940895 1106 100-101 4.528687533032873 1107 1 0.23352346968160376 1107 2 0.22201787480773305 1107 3 0.25899906687034857 1107 4 0.19794198093694604 1107 5 0.13005332333352015 1107 6 0.1467572788977094 1107 7 0.16823432538730998 1107 8 0.17702868358664148 1107 9 0.22430601319585008 1107 10-11 0.3408716509033738 1107 12-13 0.38244024737316096 1107 14-15 0.45178884171907896 1107 16-17 0.5146881257269698 1107 18-19 0.49285207358165195 1107 20-21 0.40548603010152107 1107 22-23 0.4575937544837245 1107 24-25 0.5252652697854288 1107 26-27 0.6038156098206073 1107 28-29 0.5784158660346819 1107 30-31 0.6035362730692668 1107 32-33 0.5694984436741422 1107 34-35 0.7764895780216889 1107 36-37 0.8353212428782584 1107 38-39 0.921263318026341 1107 40-41 1.0371341137623205 1107 42-43 1.025822905063528 1107 44-45 1.0787911158822254 1107 46-47 1.0935280153821907 1107 48-49 1.1521829348621324 1107 50-51 1.2184767434360353 1107 52-53 1.399808164431171 1107 54-55 1.3963689043124603 1107 56-57 1.3287763502351204 1107 58-59 1.4875925051784513 1107 60-61 1.64571356159518 1107 62-63 1.8727296966069282 1107 64-65 2.0836260466004717 1107 66-67 2.368091937790812 1107 68-69 2.4443296816988713 1107 70-71 2.407756565042053 1107 72-73 2.716064260660776 1107 74-75 2.8893186533784316 1107 76-77 2.7578295042410375 1107 78-79 3.119278068679403 1107 80-81 3.407082055009102 1107 82-83 3.564933434359272 1107 84-85 3.864892184088589 1107 86-87 4.204119795562015 1107 88-89 4.55099841950388 1107 90-91 4.643890459171953 1107 92-93 4.787429583064441 1107 94-95 4.776365841820407 1107 96-97 4.995826524304107 1107 98-99 5.261746897245267 1107 100-101 4.966058696240822 1108 1 0.1737330562790227 1108 2 0.14552621656702414 1108 3 0.1106843425099946 1108 4 0.03256789350962208 1108 5 -0.023392552545814738 1108 6 -0.03663794061918679 1108 7 0.032188923182211227 1108 8 0.046829351866399804 1108 9 0.10133947197835624 1108 10-11 0.09008379079992324 1108 12-13 0.14565349252557525 1108 14-15 0.3446704693915734 1108 16-17 0.3439958628293951 1108 18-19 0.27058084465642906 1108 20-21 0.374917345540446 1108 22-23 0.2532910777475834 1108 24-25 0.3194969432946664 1108 26-27 0.3063566802323905 1108 28-29 0.3577087993200081 1108 30-31 0.5091747547533814 1108 32-33 0.5963747975466944 1108 34-35 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2.5829703054616573 2311 88-89 2.750990115892968 2311 90-91 2.7474260594554316 2311 92-93 2.762459337670208 2311 94-95 2.9675345975854626 2311 96-97 2.8351143834594197 2311 98-99 2.8780165032614597 2311 100-101 2.4702500243100545 2312 1 -0.3117043401531383 2312 2 -0.2572709768060868 2312 3 -0.26274195761787666 2312 4 -0.23549353423766917 2312 5 -0.3090967309201531 2312 6 -0.210974005007607 2312 7 -0.2320926185862433 2312 8 -0.3156737645634209 2312 9 -0.2167151916260437 2312 10-11 -0.2980296405072309 2312 12-13 -0.2484380416183214 2312 14-15 -0.3885762258970047 2312 16-17 -0.2588621300809564 2312 18-19 -0.25420818405929424 2312 20-21 -0.26298538660312687 2312 22-23 -0.24738209700935698 2312 24-25 -0.2968353000009287 2312 26-27 -0.26372670791381836 2312 28-29 -0.3344685342185798 2312 30-31 -0.23881803119752476 2312 32-33 -0.08266688149499402 2312 34-35 -0.17243563826263397 2312 36-37 -0.24061192156246847 2312 38-39 -0.02817470339125805 2312 40-41 0.136993703140746 2312 42-43 0.1606561406273599 2312 44-45 0.28604951253305444 2312 46-47 0.31832823855001635 2312 48-49 0.38101883230731204 2312 50-51 0.467285282656249 2312 52-53 0.5435977801466123 2312 54-55 0.5538524982969548 2312 56-57 0.661267331871084 2312 58-59 0.9093340079573231 2312 60-61 1.0354092679154157 2312 62-63 1.2774746888392308 2312 64-65 1.606622907204084 2312 66-67 1.7321133253972931 2312 68-69 1.8899203719394926 2312 70-71 2.0144410387711 2312 72-73 2.005922740921978 2312 74-75 2.3068532021215447 2312 76-77 2.3340555893855637 2312 78-79 2.492860292399982 2312 80-81 2.493734423801694 2312 82-83 2.7005909456811885 2312 84-85 2.915590475505134 2312 86-87 2.9770197699928573 2312 88-89 3.096232111020889 2312 90-91 3.381122361106339 2312 92-93 3.3590588426627654 2312 94-95 3.5496178769471065 2312 96-97 3.4182747984634076 2312 98-99 3.458521162933394 2312 100-101 3.06282665366313 2313 1 -0.22480968463660034 2313 2 -0.24414689852982008 2313 3 -0.3331794691504264 2313 4 -0.24242222046353845 2313 5 -0.1316126370370867 2313 6 -0.12988020504788977 2313 7 -0.21945646405639252 2313 8 -0.2194606669659933 2313 9 -0.29131315457257756 2313 10-11 -0.3042537670969594 2313 12-13 -0.24692454835754774 2313 14-15 -0.4114439892652513 2313 16-17 -0.22655767636821622 2313 18-19 -0.20757808350249718 2313 20-21 -0.345934536328393 2313 22-23 -0.1706532707730375 2313 24-25 -0.24679170519736715 2313 26-27 -0.18106574871545433 2313 28-29 -0.1374636873779025 2313 30-31 -0.13455398050077605 2313 32-33 -0.11516620890957086 2313 34-35 0.13165318808638204 2313 36-37 0.021001269281363477 2313 38-39 -0.023608940031451198 2313 40-41 0.018318647115435738 2313 42-43 0.008453229705843057 2313 44-45 0.19675967822147555 2313 46-47 0.2587759057702499 2313 48-49 0.48376530493132464 2313 50-51 0.5378280837385887 2313 52-53 0.7074665619007448 2313 54-55 0.8490436181974559 2313 56-57 0.8323360172799958 2313 58-59 1.0575868352810538 2313 60-61 1.4954285369594444 2313 62-63 1.4623849004465725 2313 64-65 1.7789161910689337 2313 66-67 1.5578116521737968 2313 68-69 1.8040004370711173 2313 70-71 2.099678035335632 2313 72-73 2.175800633215342 2313 74-75 2.4016173183161342 2313 76-77 2.408304089625723 2313 78-79 2.6310955409467063 2313 80-81 2.733906788781052 2313 82-83 2.7496965127164756 2313 84-85 3.179253819388549 2313 86-87 3.302133053097087 2313 88-89 3.4171538840546525 2313 90-91 3.4920114838154994 2313 92-93 3.548599118204457 2313 94-95 3.5845426671074954 2313 96-97 3.584699334379472 2313 98-99 3.7066617283558863 2313 100-101 3.504781686926176 2314 1 -0.3317257181452682 2314 2 -0.3536605033093494 2314 3 -0.3568888009252156 2314 4 -0.2870225191842337 2314 5 -0.3291189686170597 2314 6 -0.33199722563364276 2314 7 -0.35653030001977015 2314 8 -0.3918679661412625 2314 9 -0.5010605407664883 2314 10-11 -0.4355090434136031 2314 12-13 -0.42381265797505563 2314 14-15 -0.3645026294367 2314 16-17 -0.4725509226829061 2314 18-19 -0.40566237469023747 2314 20-21 -0.3881158468030961 2314 22-23 -0.4094155280624747 2314 24-25 -0.4659942508172179 2314 26-27 -0.4538204730482036 2314 28-29 -0.4956590247374706 2314 30-31 -0.4145883983201202 2314 32-33 -0.4387424151567316 2314 34-35 -0.3799417206739335 2314 36-37 -0.3469576890925552 2314 38-39 -0.17355049467786188 2314 40-41 -0.25169888649064376 2314 42-43 -0.10136883666211105 2314 44-45 0.016153794722548298 2314 46-47 0.004713953695436146 2314 48-49 0.2761122105745457 2314 50-51 0.3083748724911075 2314 52-53 0.3529662696739706 2314 54-55 0.61560638201329 2314 56-57 0.8482201656013082 2314 58-59 1.0703668859336908 2314 60-61 1.080992534621629 2314 62-63 1.2150214352864985 2314 64-65 1.3704578496181945 2314 66-67 1.5640685519724862 2314 68-69 1.5442757796258277 2314 70-71 1.87396857368228 2314 72-73 1.8914672349427306 2314 74-75 1.8250084476102444 2314 76-77 1.8090768670788044 2314 78-79 2.071221523153344 2314 80-81 2.410856590716243 2314 82-83 2.202355776305019 2314 84-85 2.5329261357727297 2314 86-87 2.76235449604793 2314 88-89 2.802010043545529 2314 90-91 2.9550642794515802 2314 92-93 3.323188377247252 2314 94-95 3.6870330885634353 2314 96-97 3.608915192552079 2314 98-99 3.5229907107573375 2314 100-101 3.5866116758864983 2315 1 -0.39002218768126795 2315 2 -0.530940015498576 2315 3 -0.4771409251259975 2315 4 -0.3642624481156673 2315 5 -0.43109994448033717 2315 6 -0.30876668790565986 2315 7 -0.5115679494390122 2315 8 -0.39809704140384383 2315 9 -0.5035935671029463 2315 10-11 -0.49704710981678346 2315 12-13 -0.4618796654807724 2315 14-15 -0.693055302364165 2315 16-17 -0.49300564668789804 2315 18-19 -0.5442982026753 2315 20-21 -0.4272357387498644 2315 22-23 -0.5203217731523324 2315 24-25 -0.45037231021023416 2315 26-27 -0.5258334478962112 2315 28-29 -0.5313989294959285 2315 30-31 -0.3939773053387441 2315 32-33 -0.3848300180429831 2315 34-35 -0.4146819215514128 2315 36-37 -0.5472810027249153 2315 38-39 -0.42187696892759163 2315 40-41 -0.17991343983436536 2315 42-43 -0.21990845617937538 2315 44-45 -0.05348025654834743 2315 46-47 -0.17930332394334414 2315 48-49 -0.05600839447519235 2315 50-51 0.21830588884412805 2315 52-53 0.35495389310726466 2315 54-55 0.5070854059082777 2315 56-57 0.6974381696382004 2315 58-59 0.7795887274314488 2315 60-61 0.9725037387987214 2315 62-63 1.1584362617849209 2315 64-65 1.4545814883771513 2315 66-67 1.4682728378421714 2315 68-69 1.690441189297438 2315 70-71 2.0757681011004827 2315 72-73 2.050015405652328 2315 74-75 2.345146505552883 2315 76-77 2.288198209404488 2315 78-79 2.514216193010842 2315 80-81 2.720204887125348 2315 82-83 2.7508833395475847 2315 84-85 2.9506355644443047 2315 86-87 3.2098720818922324 2315 88-89 3.4962721330580777 2315 90-91 3.6111191581636426 2315 92-93 3.7839231452470585 2315 94-95 4.00894242175173 2315 96-97 4.038683766467017 2315 98-99 4.292848362110423 2315 100-101 4.221466547346665 2316 1 -0.568901351043813 2316 2 -0.8837854507268403 2316 3 -0.5743068899589829 2316 4 -0.3743934585555877 2316 5 -0.2825380906231061 2316 6 -0.5565537053420684 2316 7 -0.40772609536782056 2316 8 -0.42469813153051916 2316 9 -0.3547340078339971 2316 10-11 -0.4515155660813335 2316 12-13 -0.4946112137628731 2316 14-15 -0.8176470230446142 2316 16-17 -0.3855991004450985 2316 18-19 -0.38718337764785105 2316 20-21 -0.5754158911429457 2316 22-23 -0.47517184730287454 2316 24-25 -0.6622843211275438 2316 26-27 -0.44336512067634715 2316 28-29 -0.41131691926060654 2316 30-31 -0.40226525015843606 2316 32-33 -0.7914417861155911 2316 34-35 -0.5105564366309139 2316 36-37 -0.35846623466434124 2316 38-39 -0.4316806037084078 2316 40-41 -0.17175479043071107 2316 42-43 -0.06189800757191932 2316 44-45 -0.30913624148021057 2316 46-47 -0.5214143979828947 2316 48-49 -0.1364407576777964 2316 50-51 0.11487699446954025 2316 52-53 0.0911437719924777 2316 54-55 0.43245211345024615 2316 56-57 0.5505495505767648 2316 58-59 0.7254814792658237 2316 60-61 1.2044843707298583 2316 62-63 1.380451702348811 2316 64-65 1.4716901887969165 2316 66-67 1.7353852992792085 2316 68-69 1.9174300319471698 2316 70-71 1.9523123747928857 2316 72-73 2.047981781823591 2316 74-75 2.25683422611565 2316 76-77 2.389685248771805 2316 78-79 2.421668088764136 2316 80-81 2.5800924510960748 2316 82-83 2.9526538927547783 2316 84-85 3.285891139776311 2316 86-87 3.4523631691170777 2316 88-89 3.770768641496801 2316 90-91 4.066389212576372 2316 92-93 3.738262138939252 2316 94-95 4.389167979151207 2316 96-97 4.506235798887317 2316 98-99 4.669698030405414 2316 100-101 4.692041601873058 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8.0 3 19.0 4 59.0 5 117.0 6 294.0 7 742.0 8 1854.0 9 3190.0 10 4991.0 11 7541.0 12 10830.0 13 15432.0 14 20715.0 15 26597.0 16 33115.0 17 39331.0 18 40381.0 19 39138.0 20 41648.0 21 45144.0 22 49113.0 23 54852.0 24 61363.0 25 69353.0 26 78704.0 27 90807.0 28 103991.0 29 117763.0 30 133772.0 31 149471.0 32 167173.0 33 188750.0 34 213324.0 35 239655.0 36 258503.0 37 224235.0 38 77859.0 39 2125.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 40.92705201502169 20.35312985627252 15.77043573075667 22.949382397949126 2 16.87711707957538 21.19235913329769 38.35780502415252 23.57271876297441 3 20.102030854283477 24.040115545635356 29.95200912726291 25.905844472818252 4 14.420402464204072 17.57749643083984 35.58823090255245 32.413870202403636 5 14.20086757255957 36.971773235539814 34.19925811816461 14.628101073736003 6 33.096691027692245 36.27679454386536 16.494056759696456 14.132457668745948 7 29.002943767494056 32.09709647050356 20.25942979962549 18.6405299623769 8 26.272617602343683 36.01668326340498 18.963505935583218 18.74719319866813 9 25.917635001161965 16.981736696479537 19.7688401693901 37.3317881329684 10-11 25.336002594221423 26.61483200923139 26.97944339861384 21.069721997933353 12-13 25.922286682141642 23.560572734870647 27.552289297037202 22.96485128595051 14-15 22.644708435316176 25.319635568552183 25.639701848306196 26.395954147825446 16-17 22.513446803720885 28.412869420997804 26.382669100089245 22.69101467519207 18-19 22.438905051725545 27.029597325226007 28.582435635475136 21.949061987573312 20-21 22.925761851545143 26.15167389687204 28.880181503614722 22.042382747968094 22-23 22.716015067617832 25.97370402827916 28.767718023138954 22.54256288096406 24-25 22.642027377498444 26.30681343958443 28.728570411259735 22.32258877165739 26-27 22.364368392637658 26.684745775691415 28.620537816799214 22.33034801487171 28-29 22.420374898687154 26.70269709440309 28.704968186713497 22.171959820196257 30-31 22.548458838749 26.41886798376238 28.646793460387393 22.385879717101226 32-33 22.446210134855367 26.37251566982708 28.651907686470608 22.529366508846945 34-35 22.853433027217662 26.547785270645957 28.44442351214938 22.154358189986997 36-37 22.927852734428843 26.25176118351532 28.488713110862673 22.331672971193164 38-39 22.50024601145552 26.511783470097832 28.416314177879492 22.571656340567156 40-41 22.82998690937605 26.399048469767706 28.42240943290682 22.34855518794943 42-43 22.686646310529994 26.391373847788422 28.30275765030199 22.61922219137959 44-45 22.700561872067144 26.582975148106154 28.321484011503532 22.394978968323173 46-47 22.80561004856131 26.80881684071248 27.730329301106142 22.65524380962007 48-49 22.785598855112195 26.724251797214276 27.854303991754847 22.63584535591868 50-51 22.920382747202385 26.947743054159734 27.618561393957563 22.513312804680318 52-53 23.025055140605193 26.8416923849111 27.519574388418803 22.613678086064905 54-55 22.88684908278596 26.86683191941985 27.59368114003343 22.65263785776076 56-57 22.868257120421877 27.051611453319136 27.525393775323426 22.554737650935564 58-59 22.942052306334055 26.78585307043487 27.542813650597118 22.72928097263395 60-61 22.87934075534876 26.90176224052522 27.65518141747248 22.563715586653544 62-63 22.645856998521033 26.998509547814493 27.69540027236262 22.66023318130185 64-65 22.5605378951201 26.875249573213054 27.653649999866005 22.910562531800842 66-67 22.636426494167587 26.880008698461992 27.496385850297635 22.987178957072786 68-69 22.681214393282882 26.817427332673677 27.608003891338846 22.8933543827046 70-71 22.73628720818359 26.792648736063622 27.413964001732033 23.057100054020758 72-73 22.767991911050654 26.831035026015922 27.286574422985666 23.114398639947765 74-75 22.664195724358613 27.108005906677707 27.00381208127693 23.223986287686753 76-77 22.638046768727992 26.9389182602024 27.39721412166117 23.025820849408433 78-79 22.70506543173151 26.895770569139867 27.42677048146621 22.97239351766241 80-81 22.700700891553044 27.03974296686893 27.272441872173335 22.98711426940469 82-83 22.81371252369174 26.98265480342436 27.233828885766044 22.969803787117858 84-85 22.774351180461128 26.916891332717803 27.262605531465468 23.046151955355604 86-87 22.739918401163585 26.797060941080037 27.27393153975163 23.189089118004752 88-89 22.664859592853254 27.021611186246332 27.055436392054617 23.2580928288458 90-91 22.68495219163851 26.855609349148153 27.206498162188737 23.252940297024605 92-93 22.64230403971896 26.629380369428755 27.181649429554877 23.546666161297406 94-95 22.54704227746301 26.76219266841478 27.109728751484997 23.581036302637216 96-97 22.56836726763322 26.63512329251723 27.057143699422543 23.73936574042701 98-99 22.513293661960237 26.65484029420064 26.947532484238838 23.884333559600286 100-101 22.80191781115617 26.41552090097154 27.059296246156105 23.723265041716182 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 483.0 1 348.0 2 286.0 3 381.0 4 321.5 5 306.0 6 641.0 7 1540.0 8 2464.0 9 2678.5 10 2250.0 11 1908.5 12 1960.5 13 2081.5 14 2282.0 15 2481.5 16 2663.0 17 2911.5 18 3210.5 19 3578.0 20 4044.5 21 4737.5 22 5556.5 23 6833.0 24 8689.0 25 10923.0 26 14194.0 27 17763.0 28 20828.0 29 24273.0 30 28454.5 31 33574.0 32 38901.5 33 42525.5 34 45890.5 35 50078.0 36 55664.5 37 61548.5 38 65333.0 39 68580.0 40 72556.5 41 78229.5 42 83323.0 43 87409.0 44 91916.0 45 95852.0 46 98228.5 47 99922.0 48 99977.5 49 99451.0 50 99581.0 51 96856.0 52 94066.0 53 93033.0 54 92118.0 55 86576.5 56 77433.5 57 70030.5 58 63341.0 59 55597.5 60 46177.5 61 38152.5 62 32036.0 63 25566.5 64 20374.5 65 16714.5 66 13052.5 67 10214.5 68 7756.5 69 5540.5 70 4244.0 71 3249.5 72 2525.0 73 2112.5 74 1725.5 75 1357.5 76 1001.5 77 632.5 78 391.5 79 278.5 80 185.0 81 100.5 82 59.0 83 33.5 84 23.0 85 10.5 86 6.5 87 5.5 88 2.5 89 3.5 90 2.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 7.657088032392544E-5 2 0.04023799761022283 3 1.531417606478509E-4 4 0.0 5 0.09108106214530932 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0012634195253447699 26-27 0.011064492206807228 28-29 0.0 30-31 0.0 32-33 4.785680020245341E-4 34-35 0.013955042939035414 36-37 0.0030245497727950555 38-39 0.011179348527293116 40-41 0.006355383066885813 42-43 0.005130248981703005 44-45 0.0025076963306085586 46-47 1.1485632048588817E-4 48-49 0.0 50-51 0.0 52-53 0.0 54-55 1.914272008098136E-5 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 1.1485632048588817E-4 68-69 1.7228448072883226E-4 70-71 0.0 72-73 1.914272008098136E-5 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 9.762787241300495E-4 84-85 0.004575110099354546 86-87 0.001320847685587714 88-89 5.742816024294408E-5 90-91 7.848515233202359E-4 92-93 2.8714080121472046E-4 94-95 0.0 96-97 0.0 98-99 0.0 100-101 8.805651237251427E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2611959.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.43353721308125 #Duplication Level Percentage of deduplicated Percentage of total 1 76.12667476415366 42.19970858446845 2 13.573363684357004 15.04839121806979 3 4.499455440411567 7.482621917839665 4 1.9580086614292707 4.341573839874995 5 1.038720683465588 2.878998083044343 6 0.6253650978972364 2.079971965562919 7 0.4161227201080713 1.6147008003223555 8 0.29377308633102156 1.302790505066593 9 0.21687655443758377 1.0820011095954578 >10 1.105472602249612 11.465987639314223 >50 0.09701750210754043 3.71656228557127 >100 0.04569051406934598 4.625732926951904 >500 0.0024210822876994903 0.8442906219405543 >1k 8.992591354229934E-4 0.7697853681227316 >5k 1.3834755929584513E-4 0.5468831342548641 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7820 0.2993921420665485 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6442 0.2466348055233639 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4955 0.1897043560025253 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 7.657088032392546E-5 0.0 5 0.0 0.0 0.0 7.657088032392546E-5 0.0 6 3.828544016196273E-5 0.0 0.0 7.657088032392546E-5 0.0 7 3.828544016196273E-5 0.0 0.0 7.657088032392546E-5 0.0 8 3.828544016196273E-5 0.0 0.0 7.657088032392546E-5 0.0 9 3.828544016196273E-5 0.0 0.0 1.5314176064785092E-4 0.0 10-11 7.657088032392546E-5 0.0 0.0 1.5314176064785092E-4 0.0 12-13 1.1485632048588818E-4 0.0 0.0 1.5314176064785092E-4 0.0 14-15 1.1485632048588818E-4 0.0 0.0 2.10569920890795E-4 0.0 16-17 1.1485632048588818E-4 0.0 0.0 2.2971264097177636E-4 0.0 18-19 1.1485632048588818E-4 0.0 0.0 3.062835212957018E-4 0.0 20-21 1.3399904056686954E-4 0.0 0.0 3.445689614576645E-4 0.0 22-23 2.2971264097177636E-4 0.0 0.0 3.828544016196273E-4 0.0 24-25 3.2542624137668315E-4 0.0 0.0 5.168534421864968E-4 0.0 26-27 3.828544016196273E-4 0.0 0.0 8.231369634821987E-4 0.0 28-29 3.828544016196273E-4 0.0 0.0 0.002277983689636782 0.0 30-31 4.2113984178158997E-4 0.0 0.0 0.00547481794316067 0.0 32-33 4.594252819435527E-4 0.0 0.0 0.01447189638122191 0.0 34-35 4.977107221055154E-4 0.0 0.0 0.0294797889247113 0.0 36-37 5.359961622674782E-4 0.0 0.0 0.05723673304213428 0.0 38-39 5.359961622674782E-4 0.0 0.0 0.0980872976949485 0.0 40-41 5.359961622674782E-4 0.0 0.0 0.14443182301100438 0.0 42-43 5.359961622674782E-4 0.0 0.0 0.19414546706131297 0.0 44-45 5.359961622674782E-4 0.0 0.0 0.25599559564296376 0.0 46-47 5.359961622674782E-4 0.0 0.0 0.33040334859773834 0.0 48-49 5.359961622674782E-4 0.0 0.0 0.41093677197842693 0.0 50-51 5.359961622674782E-4 0.0 0.0 0.49916556883167 0.0 52-53 5.742816024294409E-4 0.0 0.0 0.5840635323908223 0.0 54-55 5.742816024294409E-4 0.0 0.0 0.6817871184042322 0.0 56-57 5.742816024294409E-4 0.0 0.0 0.7983471409773277 0.0 58-59 5.934243225104223E-4 0.0 0.0 0.9259907984773115 0.0 60-61 6.125670425914037E-4 0.0 0.0 1.0637418121800533 0.0 62-63 6.317097626723849E-4 0.0 0.0 1.2031582425298406 0.0 64-65 6.508524827533663E-4 0.0 0.0 1.3545005874900793 0.0 66-67 6.508524827533663E-4 0.0 0.0 1.5254067923730807 0.0 68-69 6.508524827533663E-4 0.0 0.0 1.7181548408684821 0.0 70-71 6.508524827533663E-4 0.0 0.0 1.930045609444865 0.0 72-73 6.508524827533663E-4 3.828544016196273E-5 0.0 2.1446737869928283 0.0 74-75 6.508524827533663E-4 3.828544016196273E-5 0.0 2.371897874354077 0.0 76-77 6.508524827533663E-4 3.828544016196273E-5 0.0 2.6198535275630284 0.0 78-79 6.508524827533663E-4 3.828544016196273E-5 0.0 2.8998923796277047 0.0 80-81 6.508524827533663E-4 3.828544016196273E-5 0.0 3.200643654820003 0.0 82-83 6.699952028343477E-4 3.828544016196273E-5 0.0 3.50761631403862 0.0 84-85 6.89137922915329E-4 3.828544016196273E-5 0.0 3.8161012481436347 0.0 86-87 6.89137922915329E-4 3.828544016196273E-5 0.0 4.141546632240399 0.0 88-89 6.89137922915329E-4 3.828544016196273E-5 0.0 4.492164693243653 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4270 0.0 50.782322 1 GTATCAA 7295 0.0 42.8268 1 ATCAACG 9445 0.0 32.66331 3 TATCAAC 9600 0.0 32.345776 2 TCAACGC 9735 0.0 31.690289 4 CAACGCA 10040 0.0 30.774933 5 AACGCAG 10420 0.0 29.628216 6 ACGCAGA 12100 0.0 25.47529 7 CGCAGAG 12105 0.0 25.386295 8 TGGTATC 2130 0.0 23.664747 2 GCAGAGT 12985 0.0 23.007452 9 GAGTACT 8290 0.0 21.972055 12-13 GTGGTAT 2465 0.0 21.799026 1 GGACCGA 970 0.0 21.055061 6 AGAGTAC 11965 0.0 20.542698 10-11 GTACATG 7460 0.0 20.461704 1 TACATGG 7190 0.0 20.370298 2 GTACTTT 8865 0.0 20.332598 14-15 CAGAGTA 12475 0.0 20.064573 10-11 AGTACTT 8955 0.0 20.04869 12-13 >>END_MODULE