FastQCFastQC Report
Thu 26 May 2016
SRR938292_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938292_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2555022
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73770.28872549825402677No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56130.2196849968415145No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39480.15451921744705133No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA34500.057.0809441
GTATCAA62550.044.4428021
ATCAACG79650.034.5505943
TATCAAC81400.033.9829672
TCAACGC82300.033.438094
CAACGCA84750.032.5268865
AACGCAG87250.031.5329996
TGGTATC15250.028.67352
ACGCAGA102450.026.8488187
CGCAGAG102900.026.7314038
GCAGAGT113500.024.2330049
GTACATG74800.024.2237971
TACATGG73000.024.0902672
GTGGTAT21200.022.7167241
ACATGGG75650.022.6181093
GAGTACT69550.022.53670312-13
CCTATAC5200.021.0226363
CAGAGTA108950.020.99145710-11
GTACTTT75900.020.5276714-15
AGAGTAC104700.020.43721210-11
Produced by FastQC (version 0.11.5)