##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938292_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2555022 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.63855262303025 31.0 31.0 34.0 30.0 34.0 2 31.84709681560472 33.0 31.0 34.0 30.0 34.0 3 31.91819287661711 33.0 31.0 34.0 30.0 34.0 4 35.5846885075745 37.0 35.0 37.0 33.0 37.0 5 35.575110116468665 37.0 35.0 37.0 33.0 37.0 6 35.564370482915606 37.0 35.0 37.0 33.0 37.0 7 35.50705825624985 37.0 35.0 37.0 33.0 37.0 8 35.46941357060722 37.0 35.0 37.0 33.0 37.0 9 37.04299101925541 39.0 37.0 39.0 33.0 39.0 10-11 36.89914176864231 39.0 37.0 39.0 33.0 39.0 12-13 36.734589760870946 39.0 37.0 39.0 32.5 39.0 14-15 38.01616796254592 40.0 38.0 41.0 33.0 41.0 16-17 37.95141235574488 40.0 38.0 41.0 33.0 41.0 18-19 37.93469684409762 40.0 38.0 41.0 33.0 41.0 20-21 37.86127223170681 40.0 38.0 41.0 33.0 41.0 22-23 37.71558033551179 40.0 37.0 41.0 32.5 41.0 24-25 37.590938942991485 40.0 37.0 41.0 32.0 41.0 26-27 37.38741329820252 39.5 37.0 41.0 32.0 41.0 28-29 37.16489877582268 39.0 36.5 41.0 31.0 41.0 30-31 36.91125027494871 39.0 36.0 40.5 31.0 41.0 32-33 36.6369498971046 39.0 36.0 40.0 30.0 41.0 34-35 36.33977613500001 38.5 35.0 40.0 30.0 41.0 36-37 36.146668795806846 38.5 35.0 40.0 30.0 41.0 38-39 35.91998503339697 38.0 35.0 40.0 29.0 41.0 40-41 35.650361718998894 38.0 35.0 40.0 27.5 41.0 42-43 35.25140351041987 38.0 34.0 40.0 26.5 41.0 44-45 34.797973168137105 38.0 33.5 40.0 25.5 41.0 46-47 34.32140095075502 38.0 33.0 40.0 24.0 41.0 48-49 33.843741854277575 38.0 33.0 40.0 22.5 41.0 50-51 33.13525754377066 36.5 32.0 39.5 21.5 40.5 52-53 33.14492223550326 37.0 32.0 39.0 21.5 40.0 54-55 33.1137761240412 37.0 32.0 40.0 19.0 40.5 56-57 32.68065871839851 37.0 31.5 39.5 16.5 41.0 58-59 32.21848402870895 36.0 31.0 39.0 11.0 40.5 60-61 31.667850218119455 36.0 30.5 39.0 7.5 40.0 62-63 30.945551545153037 35.0 29.0 38.5 4.0 40.0 64-65 30.280723023128566 35.0 28.5 38.0 2.0 40.0 66-67 29.567436014249584 34.0 28.0 38.0 2.0 40.0 68-69 28.693288550940068 34.0 26.0 37.0 2.0 39.0 70-71 27.838323309936275 33.0 25.5 36.0 2.0 39.0 72-73 26.921905956191374 32.5 23.0 36.0 2.0 38.5 74-75 25.958196837444063 31.5 20.5 35.0 2.0 38.0 76-77 24.986057262912023 31.0 16.0 35.0 2.0 37.0 78-79 23.98401109657764 30.5 7.5 34.0 2.0 36.0 80-81 22.98861868899759 29.5 2.0 34.0 2.0 36.0 82-83 21.958401140968647 29.0 2.0 34.0 2.0 35.0 84-85 20.947248008040635 27.5 2.0 33.5 2.0 35.0 86-87 19.919872509904025 26.0 2.0 33.0 2.0 35.0 88-89 18.797043626238835 25.0 2.0 32.5 2.0 34.5 90-91 17.607547801936732 21.5 2.0 32.0 2.0 34.0 92-93 16.397886202154034 15.0 2.0 31.0 2.0 34.0 94-95 15.08740257422441 2.0 2.0 31.0 2.0 34.0 96-97 13.614954587475177 2.0 2.0 30.0 2.0 34.0 98-99 11.835007291522343 2.0 2.0 29.0 2.0 34.0 100-101 8.304425363069281 2.0 2.0 11.0 2.0 28.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.43090282518499734 1101 2 0.33136151657300417 1101 3 0.3375866625718409 1101 4 0.17162925262417872 1101 5 0.17624600975648974 1101 6 0.2990650857263262 1101 7 0.3037540283740583 1101 8 0.2464249850588942 1101 9 0.35107564599982055 1101 10-11 0.45227400236023385 1101 12-13 0.3930520834402529 1101 14-15 0.560293050919789 1101 16-17 0.5702960742142125 1101 18-19 0.5509977722005885 1101 20-21 0.7078881661933778 1101 22-23 0.6485558233282518 1101 24-25 0.7328314884550409 1101 26-27 0.7928114714027998 1101 28-29 0.8697247231206546 1101 30-31 0.8286915608893608 1101 32-33 0.8426227853359478 1101 34-35 0.8520682001786071 1101 36-37 1.003846432020957 1101 38-39 1.045674151255433 1101 40-41 0.9978754951983788 1101 42-43 1.150274431439378 1101 44-45 1.2780005861001484 1101 46-47 1.3993777182841782 1101 48-49 1.3142866429679074 1101 50-51 1.563249970881536 1101 52-53 1.4559331463090857 1101 54-55 1.7681960344439034 1101 56-57 1.8441253608405148 1101 58-59 1.9797402705321403 1101 60-61 2.037812208198435 1101 62-63 1.8861327849017542 1101 64-65 2.115928790582249 1101 66-67 2.3017595547512606 1101 68-69 2.333217309796421 1101 70-71 2.553701514267537 1101 72-73 2.8301261036658367 1101 74-75 2.811722495543613 1101 76-77 3.114248269657626 1101 78-79 3.238474071316382 1101 80-81 3.6048045153078228 1101 82-83 3.7216776454958485 1101 84-85 3.7663850825080907 1101 86-87 4.061317271507068 1101 88-89 4.102388088432857 1101 90-91 3.9993106109131666 1101 92-93 4.160645623418304 1101 94-95 4.057625082678513 1101 96-97 4.162417219542041 1101 98-99 3.7614462119950733 1101 100-101 2.8191910223341363 1102 1 0.5486570244577997 1102 2 0.3668493466995102 1102 3 0.3280131460352358 1102 4 0.20187010610531786 1102 5 0.31220041823176103 1102 6 0.3435543055743082 1102 7 0.44865434596668763 1102 8 0.49232868967474985 1102 9 0.5212242392958686 1102 10-11 0.4770498810173862 1102 12-13 0.5571673640164931 1102 14-15 0.5717450595206302 1102 16-17 0.6982516680799478 1102 18-19 0.6535205835245819 1102 20-21 0.5400707055000353 1102 22-23 0.48142814892631947 1102 24-25 0.6094212445704343 1102 26-27 0.5675930334389037 1102 28-29 0.5256181164632494 1102 30-31 0.4788835102477478 1102 32-33 0.6319571128095305 1102 34-35 0.6089670779819016 1102 36-37 0.8099271062320739 1102 38-39 1.0424516077268393 1102 40-41 1.069912729449662 1102 42-43 1.1418203375797233 1102 44-45 1.0331361279223685 1102 46-47 1.074075315162112 1102 48-49 1.059156581546823 1102 50-51 1.1795633659291482 1102 52-53 1.2838037601275474 1102 54-55 1.157659049855333 1102 56-57 1.3249225088668268 1102 58-59 1.554649505616993 1102 60-61 1.4805105547411124 1102 62-63 1.648928303692763 1102 64-65 1.9827268966638805 1102 66-67 2.0873902136037294 1102 68-69 2.1041115759436444 1102 70-71 1.7798250979898 1102 72-73 2.0714566503392327 1102 74-75 2.3171811780551828 1102 76-77 2.2680324528139835 1102 78-79 2.5564784745818905 1102 80-81 3.0498008414092794 1102 82-83 3.0619735465617524 1102 84-85 3.1935042724371456 1102 86-87 3.357509881219965 1102 88-89 3.657265171831032 1102 90-91 3.445198415599787 1102 92-93 3.4768764064932043 1102 94-95 3.437330683648476 1102 96-97 3.195670805145225 1102 98-99 3.042580641498846 1102 100-101 2.0634974944849045 1103 1 0.302430839767851 1103 2 0.24420500716109217 1103 3 0.2954253758589651 1103 4 0.19210404284339688 1103 5 0.1403228253129427 1103 6 0.23378947551965723 1103 7 0.24801514246845358 1103 8 0.2448091118391318 1103 9 0.3315034776348895 1103 10-11 0.31282567964643704 1103 12-13 0.2508558360217492 1103 14-15 0.3531488836963703 1103 16-17 0.3879056756952721 1103 18-19 0.4831871135554593 1103 20-21 0.4412743157947361 1103 22-23 0.5526151055611237 1103 24-25 0.41236477627766277 1103 26-27 0.517258539371575 1103 28-29 0.5598477278608698 1103 30-31 0.5024836122286231 1103 32-33 0.5901635844131974 1103 34-35 0.6192017600061064 1103 36-37 0.8121857521544129 1103 38-39 0.8994753665372386 1103 40-41 0.838960084876895 1103 42-43 1.0072203886773963 1103 44-45 1.0087219750995757 1103 46-47 1.114063267263269 1103 48-49 1.0153874417008524 1103 50-51 1.2230981963939698 1103 52-53 1.275925174208787 1103 54-55 1.400926207320623 1103 56-57 1.5448874777377242 1103 58-59 1.561281613884013 1103 60-61 1.659518833583224 1103 62-63 1.7364954029024133 1103 64-65 1.8626740349661937 1103 66-67 1.9422540500954568 1103 68-69 2.0537252067444456 1103 70-71 2.068857046368816 1103 72-73 2.405719558333125 1103 74-75 2.2754929414316614 1103 76-77 2.2517297878262674 1103 78-79 2.6036530760883885 1103 80-81 2.6639137324197613 1103 82-83 2.9717850106173813 1103 84-85 2.9402244102561 1103 86-87 3.061930540551547 1103 88-89 3.595718075779466 1103 90-91 3.4921271128959255 1103 92-93 3.432908143886543 1103 94-95 3.351769959048056 1103 96-97 3.0496986075350225 1103 98-99 2.7559008349193643 1103 100-101 1.6884849948568768 1104 1 0.4571898990448666 1104 2 0.3569719858120308 1104 3 0.356117909880286 1104 4 0.23117745325610173 1104 5 0.25774435742427926 1104 6 0.34007595168051097 1104 7 0.35592485549859987 1104 8 0.3907803423648062 1104 9 0.49950733891664356 1104 10-11 0.4536843940176922 1104 12-13 0.47133571728887347 1104 14-15 0.5801245991644564 1104 16-17 0.5354173261917978 1104 18-19 0.5143779718015367 1104 20-21 0.46896293657327703 1104 22-23 0.47310793218077407 1104 24-25 0.5933977692213546 1104 26-27 0.6388761490539281 1104 28-29 0.6217861718756836 1104 30-31 0.6851032768734271 1104 32-33 0.7066857416862717 1104 34-35 0.6457125333234899 1104 36-37 0.7384384908534329 1104 38-39 0.8480308050414891 1104 40-41 0.8278250930758233 1104 42-43 0.9064727657019986 1104 44-45 0.9575955947163592 1104 46-47 1.0445821988470172 1104 48-49 1.1516808344519447 1104 50-51 1.1413311673215887 1104 52-53 1.2883512371935169 1104 54-55 1.486447003727946 1104 56-57 1.5951099928234314 1104 58-59 1.686938519960158 1104 60-61 1.826645900621795 1104 62-63 1.8029957044688274 1104 64-65 1.9375478883434631 1104 66-67 2.3211015878419374 1104 68-69 2.4291376714638666 1104 70-71 2.4129661989126667 1104 72-73 2.6456768065950946 1104 74-75 2.5468157909539357 1104 76-77 2.6445707512838084 1104 78-79 2.845572526743563 1104 80-81 2.989851086920865 1104 82-83 3.1603556243079183 1104 84-85 3.344893949020502 1104 86-87 3.3517884662698556 1104 88-89 3.36331345922812 1104 90-91 3.0855887091412875 1104 92-93 3.103124711038216 1104 94-95 3.2810615839694854 1104 96-97 3.4111703773648596 1104 98-99 3.100919369610663 1104 100-101 2.338328027646339 1105 1 0.40387336393457574 1105 2 0.24867097531945603 1105 3 0.2096001993399561 1105 4 0.13036472225268625 1105 5 0.19766249022232785 1105 6 0.23737212098758675 1105 7 0.29604461153077466 1105 8 0.2554477085861251 1105 9 0.29900175844595367 1105 10-11 0.339575297653667 1105 12-13 0.3061684145621655 1105 14-15 0.33670705935407597 1105 16-17 0.4477130759146064 1105 18-19 0.43317263617497304 1105 20-21 0.4451637830195452 1105 22-23 0.3617361589879664 1105 24-25 0.448385549293846 1105 26-27 0.5298792970498383 1105 28-29 0.41661986426917963 1105 30-31 0.5674791411984472 1105 32-33 0.48629502333573527 1105 34-35 0.511149818100904 1105 36-37 0.6481478563454885 1105 38-39 0.7364744890358708 1105 40-41 0.7285007722639278 1105 42-43 0.9155747736911835 1105 44-45 0.802032501496619 1105 46-47 1.0035993988312768 1105 48-49 1.0510346375562776 1105 50-51 1.046018888949341 1105 52-53 1.1953333049790231 1105 54-55 1.3886797861062945 1105 56-57 1.4800393801338672 1105 58-59 1.6022504492977134 1105 60-61 1.510794564153091 1105 62-63 1.5923105642125748 1105 64-65 1.7402565832433226 1105 66-67 1.9123327594622985 1105 68-69 2.2098828011302594 1105 70-71 2.2174753930064064 1105 72-73 2.140615246528821 1105 74-75 2.339805540473268 1105 76-77 2.3703559122593525 1105 78-79 2.8851294828936673 1105 80-81 2.9692480143758573 1105 82-83 3.2513623369134947 1105 84-85 3.241607341778849 1105 86-87 3.198473119362788 1105 88-89 3.359801947395475 1105 90-91 3.1929907696341964 1105 92-93 3.473344328124874 1105 94-95 3.5769165741630964 1105 96-97 3.3618952184612443 1105 98-99 3.1106308887210563 1105 100-101 2.2411703211459013 1106 1 0.10986971441521476 1106 2 0.13565511092823002 1106 3 0.060913981029873554 1106 4 0.07940502544352768 1106 5 0.07915197106385818 1106 6 -0.08097750538192372 1106 7 0.1026895139008488 1106 8 -0.12597687178706707 1106 9 0.16691345758549403 1106 10-11 0.044458152308315846 1106 12-13 0.21625824888485567 1106 14-15 0.25749113247728417 1106 16-17 -0.07691740647697998 1106 18-19 0.24962316918893634 1106 20-21 0.21394265806802792 1106 22-23 0.09004846004162914 1106 24-25 0.26442215315798734 1106 26-27 0.18117926852381316 1106 28-29 0.21441754988829587 1106 30-31 0.07016458674016235 1106 32-33 0.2535615665405828 1106 34-35 0.173576446612131 1106 36-37 0.24151699058833032 1106 38-39 0.31519347453589575 1106 40-41 0.468933613108355 1106 42-43 0.15621665441619115 1106 44-45 0.4711450102317727 1106 46-47 0.46080282255737615 1106 48-49 0.3560819318859316 1106 50-51 0.1604859579989082 1106 52-53 0.5012952431183137 1106 54-55 0.3684082002859981 1106 56-57 0.8948824191058407 1106 58-59 0.9308234087439686 1106 60-61 1.0875280481158889 1106 62-63 1.1235974952203591 1106 64-65 1.1589638413796521 1106 66-67 1.3388771264789732 1106 68-69 1.4366586213287924 1106 70-71 1.2636313231735024 1106 72-73 1.4669453665343148 1106 74-75 1.8299040684400865 1106 76-77 1.9287968973579055 1106 78-79 2.36781652830739 1106 80-81 2.1816600780145414 1106 82-83 2.1692187197236237 1106 84-85 2.3450903838901986 1106 86-87 2.388929958287374 1106 88-89 2.3061541637464664 1106 90-91 2.2764074925227824 1106 92-93 2.391571624658134 1106 94-95 2.331165290869535 1106 96-97 2.252599811150432 1106 98-99 2.3275512709395354 1106 100-101 1.7709713332913442 1107 1 0.11256404432336709 1107 2 0.1501472430937092 1107 3 0.07512927013903337 1107 4 0.08669773356292865 1107 5 0.11273273287877572 1107 6 0.09312330009596081 1107 7 0.09501238512444132 1107 8 0.06127435501133505 1107 9 0.2032141271996366 1107 10-11 0.14326498130431276 1107 12-13 0.18642182702117793 1107 14-15 0.2232782582219599 1107 16-17 0.165547459656608 1107 18-19 0.2450251671231456 1107 20-21 0.296192504412609 1107 22-23 0.3108236506909492 1107 24-25 0.32131622232211754 1107 26-27 0.2811427447999435 1107 28-29 0.3383824916271294 1107 30-31 0.354737432197787 1107 32-33 0.4688931801368881 1107 34-35 0.40527377289498645 1107 36-37 0.566330441248958 1107 38-39 0.7200162611267658 1107 40-41 0.7489793599188346 1107 42-43 0.7963342224402155 1107 44-45 0.7011171453763225 1107 46-47 0.8304599584504544 1107 48-49 0.6923584153471936 1107 50-51 1.024324499818384 1107 52-53 1.0477629363611598 1107 54-55 1.145545778496036 1107 56-57 1.2577760835374434 1107 58-59 1.4075655828496707 1107 60-61 1.61413714195891 1107 62-63 1.7282108816353343 1107 64-65 1.7708580873321402 1107 66-67 1.932431272241356 1107 68-69 2.0336008972946793 1107 70-71 2.2611987905033892 1107 72-73 2.13976144720397 1107 74-75 2.3140929822298055 1107 76-77 2.499771407484868 1107 78-79 2.7548213046024053 1107 80-81 2.952395472474521 1107 82-83 3.067125695942952 1107 84-85 2.7320359172757414 1107 86-87 2.6969210556766967 1107 88-89 3.007760605764023 1107 90-91 3.0044900609643257 1107 92-93 2.9012812554111846 1107 94-95 2.560441368845993 1107 96-97 2.6583930530202355 1107 98-99 2.545181885568736 1107 100-101 1.7137923339435819 1108 1 0.23843193467136814 1108 2 0.21573903219778146 1108 3 0.061144583656332685 1108 4 0.17334092997102601 1108 5 0.14112479411728884 1108 6 0.21777246761395475 1108 7 0.10023728858550385 1108 8 0.13964997297114223 1108 9 0.3187728231418987 1108 10-11 0.2424237246926637 1108 12-13 0.17109165983933394 1108 14-15 0.1840895377521079 1108 16-17 0.11149574098762116 1108 18-19 0.19838216030495914 1108 20-21 0.24900242132604689 1108 22-23 0.2846957912558281 1108 24-25 0.4092576842499085 1108 26-27 0.32275785151841063 1108 28-29 0.3531951148149943 1108 30-31 0.3946239893738479 1108 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0.6667131174462853 2316 72-73 0.744602468251891 2316 74-75 0.8685198689719655 2316 76-77 0.8114045581328 2316 78-79 0.7915371612690016 2316 80-81 0.7936953111142628 2316 82-83 1.0351119625499123 2316 84-85 1.1244453742664788 2316 86-87 1.216630109622205 2316 88-89 0.9633305202501923 2316 90-91 0.8669336381862323 2316 92-93 0.8737728852919773 2316 94-95 0.9162013614532754 2316 96-97 0.7674028554783145 2316 98-99 0.4146891325636677 2316 100-101 0.26843140587329195 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6295.0 3 1929.0 4 1965.0 5 2371.0 6 2838.0 7 3481.0 8 4460.0 9 6056.0 10 7999.0 11 10368.0 12 13289.0 13 17080.0 14 20532.0 15 24484.0 16 29112.0 17 35142.0 18 35149.0 19 35132.0 20 39244.0 21 44369.0 22 50301.0 23 57287.0 24 64346.0 25 73231.0 26 82255.0 27 93165.0 28 103871.0 29 117125.0 30 132090.0 31 150576.0 32 171791.0 33 196741.0 34 223085.0 35 244576.0 36 240992.0 37 169613.0 38 42071.0 39 611.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.850061256963826 18.399085878767384 14.51591679422806 24.23493607004073 2 15.566947953185467 19.347227360545897 40.28621930952674 24.799605376741894 3 19.43906221431712 23.132527101578702 29.07782398078136 28.350586703322815 4 13.062745942559564 16.011942285479357 36.297382485953136 34.62792928600795 5 14.37545869457775 36.51377239036563 34.01424430239653 15.096524612660087 6 33.36780585223546 35.095791441913285 17.12463166550823 14.411771040343027 7 29.40133173121534 31.62100121411727 20.27661984425941 18.70104721040797 8 26.353255326354862 35.1015350160236 19.36477235130939 19.180437306312147 9 26.188512972785887 16.906397360182545 19.819303430227915 37.085786236803656 10-11 25.21804532179169 26.07920517236215 27.200592299441666 21.502157206404494 12-13 25.86866699343386 23.23950912886268 27.607292911292102 23.28453096641136 14-15 22.779053268035714 24.92742298399733 25.63771957348928 26.65580417447767 16-17 22.679356422378298 28.026231341026808 26.33449226786518 22.95991996872971 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26.32489334955687 28.137632844559167 23.046712081870275 44-45 22.526406105936783 26.535687605819646 28.186664928733997 22.751241359509574 46-47 22.631759843478303 26.675178329981126 27.672600951876152 23.02046087466442 48-49 22.702800777187925 26.607295208691635 27.749864019514643 22.940039994605797 50-51 22.736202596255765 26.91534925448849 27.52086696342038 22.82758118583536 52-53 22.81957938382249 26.73428971627715 27.520261374718448 22.925869525181916 54-55 22.640611392169717 26.770542013640856 27.66661121603241 22.922235378157016 56-57 22.639787193734804 26.92509272697952 27.587391948996636 22.84772813028904 58-59 22.73888843008039 26.624322338759583 27.61748183020493 23.019307400955096 60-61 22.605629874914502 26.807398861578115 27.694514653977087 22.892456609530296 62-63 22.412389022703046 26.82525519665266 27.849329806041347 22.91302597460295 64-65 22.36740389338688 26.738706262023726 27.765030755290436 23.12885908929896 66-67 22.435203957822885 26.786793427634308 27.575833483369472 23.202169131173335 68-69 22.520252363411768 26.608045678732562 27.71870145333238 23.15300050452329 70-71 22.530890353410342 26.541151354462706 27.647299324016334 23.280658968110615 72-73 22.5671650086906 26.574470554411615 27.53661139557882 23.32175304131896 74-75 22.42835930232581 26.91671244748254 27.280760451481374 23.374167798710282 76-77 22.56225101745745 26.708013392315465 27.31574802594859 23.413987564278496 78-79 22.647365224799355 26.660711863803066 27.36563970454836 23.326283206849226 80-81 22.616905726816377 26.85254572687118 27.124097494032224 23.40645105228022 82-83 22.704575768458817 26.7385992329846 27.109287462459008 23.447537536097578 84-85 22.790136856758654 26.57039651843013 27.09655790446663 23.542908720344588 86-87 22.72004258383693 26.493467608103455 27.11500375739738 23.671486050662242 88-89 22.709159525741583 26.584772576655585 27.01358038174444 23.692487515858392 90-91 22.799777560854157 26.508251525354325 26.937881656658796 23.754089257132723 92-93 22.82892330346821 26.164202674728994 27.033970071985138 23.972903949817663 94-95 22.691837258376392 26.260733199700688 26.92539935655117 24.122030185371745 96-97 22.793837871862685 26.098481467737063 26.84580822149464 24.26187243890562 98-99 22.710974277236556 26.068823281086427 26.67715819154085 24.543044250136166 100-101 23.621395290697482 25.192188140125076 26.690273067132082 24.496143502045364 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 382.0 1 285.5 2 255.5 3 334.0 4 297.5 5 286.5 6 533.0 7 1224.5 8 2052.0 9 2368.0 10 2094.5 11 1795.5 12 1772.0 13 1890.5 14 2090.0 15 2317.0 16 2442.0 17 2668.5 18 2996.0 19 3264.0 20 3665.5 21 4279.5 22 5090.0 23 6245.0 24 7851.5 25 10106.0 26 13200.5 27 16739.5 28 19701.5 29 22811.5 30 26736.5 31 31396.5 32 36548.5 33 40191.0 34 43565.5 35 47833.5 36 53325.0 37 58906.5 38 62595.0 39 65669.5 40 69746.0 41 75605.5 42 80112.5 43 83714.0 44 88303.0 45 92265.0 46 95135.0 47 96915.5 48 97315.0 49 97010.0 50 96453.0 51 94455.0 52 92888.5 53 92290.0 54 90865.5 55 85891.5 56 78093.0 57 70960.5 58 64548.5 59 57243.5 60 48231.0 61 40102.0 62 33421.0 63 26758.5 64 21527.0 65 17918.0 66 14262.0 67 10997.0 68 8345.0 69 6156.0 70 4744.5 71 3637.0 72 2812.0 73 2251.0 74 1816.5 75 1512.0 76 1099.5 77 704.0 78 461.5 79 290.5 80 179.5 81 125.0 82 85.5 83 54.0 84 32.0 85 19.5 86 12.0 87 9.0 88 7.5 89 7.0 90 5.5 91 3.5 92 2.5 93 2.5 94 3.0 95 1.0 96 0.0 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3596446527661993 2 0.02688822248888659 3 0.03268073621283887 4 0.0419565858924111 5 0.061838997863814874 6 0.050762772297068284 7 0.03534216143735749 8 0.038238418299333624 9 0.03545957725608625 10-11 0.03555742377169355 12-13 0.027631856007502086 14-15 0.026085881060906715 16-17 0.02037164454944028 18-19 0.02514655451107662 20-21 0.03250461248474573 22-23 0.031467439419308324 24-25 0.039686546730321695 26-27 0.03479422094995659 28-29 0.029999741685198797 30-31 0.032406765969138426 32-33 0.027729702523109388 34-35 0.02859075186045365 36-37 0.030821652416300135 38-39 0.013972482428722727 40-41 0.011350195810447033 42-43 0.011643735357268939 44-45 0.010273884138766712 46-47 0.019099639846545353 48-49 0.016731754168848645 50-51 0.010410869260616934 52-53 0.00870833988904988 54-55 0.0077103054298553984 56-57 0.008219107311013368 58-59 0.0037181675930774765 60-61 0.006633993758175077 62-63 0.010821824626167603 64-65 0.006927533304996982 66-67 0.003209365711919506 68-69 0.005283711842794308 70-71 0.011761151175997702 72-73 0.009804220863851661 74-75 0.010821824626167603 76-77 0.0048531871741221795 78-79 0.008669201282806958 80-81 0.010195606926280871 82-83 0.012309091663398594 84-85 0.007729874732976859 86-87 0.0024265935870610897 88-89 0.014911808978552828 90-91 0.024089812142517757 92-93 0.010117329713795028 94-95 0.01982370406203939 96-97 0.017612372809314363 98-99 0.01023474553252379 100-101 0.11860954621917148 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2555022.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.36903683281751 #Duplication Level Percentage of deduplicated Percentage of total 1 76.92429538959743 43.361484401547514 2 13.191658699814573 14.872021902716106 3 4.2780825234104185 7.234541740078643 4 1.938683033233534 4.371267812299978 5 0.9755643356381304 2.749581098418446 6 0.5831377834970477 1.9722549117931587 7 0.39049264399623446 1.5408185962657626 8 0.27894456436945875 1.2579069138605004 9 0.19514544155406136 0.9900144524445589 >10 1.1055523059563932 11.477482861243773 >50 0.08767859443668513 3.4094433084767553 >100 0.04776396581124326 4.8214853094885735 >500 0.0020935246638137425 0.8145048052422179 >1k 7.676257100591873E-4 0.6162087208561056 >5k 1.3956831091985225E-4 0.5109831652678463 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7377 0.28872549825402677 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5613 0.2196849968415145 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3948 0.15451921744705133 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 3.9138606242920804E-5 0.0 5 0.0 0.0 0.0 3.9138606242920804E-5 0.0 6 0.0 0.0 0.0 3.9138606242920804E-5 0.0 7 0.0 0.0 0.0 1.5655442497168322E-4 0.0 8 3.9138606242920804E-5 0.0 0.0 1.9569303121460402E-4 0.0 9 3.9138606242920804E-5 0.0 0.0 3.9138606242920804E-4 0.0 10-11 1.5655442497168322E-4 0.0 0.0 4.6966327491504965E-4 0.0 12-13 1.9569303121460402E-4 0.0 0.0 4.6966327491504965E-4 0.0 14-15 1.9569303121460402E-4 0.0 0.0 4.6966327491504965E-4 0.0 16-17 2.5440094057898525E-4 0.0 0.0 5.088018811579705E-4 0.0 18-19 3.1310884994336643E-4 0.0 0.0 5.283711842794308E-4 0.0 20-21 3.1310884994336643E-4 0.0 0.0 6.066483967652725E-4 0.0 22-23 3.1310884994336643E-4 0.0 0.0 6.84925609251114E-4 0.0 24-25 3.718167593077476E-4 0.0 0.0 8.414800342227973E-4 0.0 26-27 3.9138606242920804E-4 0.0 0.0 0.001076311671680322 0.0 28-29 3.9138606242920804E-4 0.0 0.0 0.0019177917059031194 0.0 30-31 3.9138606242920804E-4 0.0 0.0 0.005890360239559581 0.0 32-33 4.3052466867212884E-4 0.0 0.0 0.015166209919131812 0.0 34-35 4.500939717935892E-4 0.0 0.0 0.030391127747628005 0.0 36-37 4.6966327491504965E-4 0.0 0.0 0.0558899297148909 0.0 38-39 4.6966327491504965E-4 0.0 0.0 0.09647666438879979 0.0 40-41 5.479404874008913E-4 0.0 0.0 0.14160347738688744 0.0 42-43 5.479404874008913E-4 0.0 0.0 0.18999836400625905 0.0 44-45 5.87079093643812E-4 0.0 0.0 0.250565357167179 0.0 46-47 6.262176998867329E-4 0.0 0.0 0.32436119923820617 0.0 48-49 6.262176998867329E-4 0.0 0.0 0.40479103506740843 0.0 50-51 6.262176998867329E-4 0.0 0.0 0.4935378247232314 0.0 52-53 7.044949123725745E-4 0.0 0.0 0.5817366738916534 0.0 54-55 7.044949123725745E-4 0.0 0.0 0.6835557580326119 0.0 56-57 7.044949123725745E-4 0.0 0.0 0.8026545368298199 0.0 58-59 7.044949123725745E-4 0.0 0.0 0.9327904025875315 0.0 60-61 7.044949123725745E-4 0.0 0.0 1.075117944189913 0.0 62-63 7.044949123725745E-4 0.0 0.0 1.220909252444793 0.0 64-65 7.044949123725745E-4 0.0 0.0 1.3767983211103467 3.9138606242920804E-5 66-67 7.044949123725745E-4 0.0 0.0 1.5513565049537734 3.9138606242920804E-5 68-69 7.436335186154953E-4 0.0 0.0 1.756051415604249 5.8707909364381206E-5 70-71 7.436335186154953E-4 0.0 0.0 1.9690045721719813 7.827721248584161E-5 72-73 7.436335186154953E-4 0.0 0.0 2.1882981829510664 7.827721248584161E-5 74-75 7.436335186154953E-4 0.0 0.0 2.4171024750471815 7.827721248584161E-5 76-77 7.436335186154953E-4 0.0 0.0 2.6661414265708867 7.827721248584161E-5 78-79 7.436335186154953E-4 0.0 0.0 2.9434775904082233 7.827721248584161E-5 80-81 7.436335186154953E-4 0.0 0.0 3.240598319701357 7.827721248584161E-5 82-83 7.632028217369558E-4 0.0 0.0 3.550086848567253 7.827721248584161E-5 84-85 7.827721248584161E-4 0.0 0.0 3.856248595902501 7.827721248584161E-5 86-87 7.827721248584161E-4 0.0 0.0 4.1841518390056915 7.827721248584161E-5 88-89 7.827721248584161E-4 0.0 0.0 4.531878786170921 7.827721248584161E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3450 0.0 57.080944 1 GTATCAA 6255 0.0 44.442802 1 ATCAACG 7965 0.0 34.550594 3 TATCAAC 8140 0.0 33.982967 2 TCAACGC 8230 0.0 33.43809 4 CAACGCA 8475 0.0 32.526886 5 AACGCAG 8725 0.0 31.532999 6 TGGTATC 1525 0.0 28.6735 2 ACGCAGA 10245 0.0 26.848818 7 CGCAGAG 10290 0.0 26.731403 8 GCAGAGT 11350 0.0 24.233004 9 GTACATG 7480 0.0 24.223797 1 TACATGG 7300 0.0 24.090267 2 GTGGTAT 2120 0.0 22.716724 1 ACATGGG 7565 0.0 22.618109 3 GAGTACT 6955 0.0 22.536703 12-13 CCTATAC 520 0.0 21.022636 3 CAGAGTA 10895 0.0 20.991457 10-11 GTACTTT 7590 0.0 20.52767 14-15 AGAGTAC 10470 0.0 20.437212 10-11 >>END_MODULE