FastQCFastQC Report
Thu 26 May 2016
SRR938292_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938292_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2555022
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75360.2949485366466512No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65580.2566709797410746No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47330.18524302334774417No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA39250.057.50041
GTATCAA71350.043.5512541
ATCAACG94000.032.4399073
TATCAAC95950.031.8821262
TCAACGC96750.031.4687484
CAACGCA97900.031.0990945
AACGCAG103200.029.5946
TGGTATC16950.027.1836932
CGCAGAG118200.025.5973118
ACGCAGA119550.025.4671787
GTGGTAT20900.023.4157371
GCAGAGT130150.022.6266339
GAGTACT83950.021.69786812-13
TAAGGTG11250.021.110085
TACATGG72500.020.5730192
AGAGTAC120850.020.4179110-11
GTACATG74250.020.349211
GTACTTT88450.020.19121414-15
AGTACTT89300.020.10540212-13
CAGAGTA125250.020.0988210-11
Produced by FastQC (version 0.11.5)