##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938292_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2555022 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.418424185780005 31.0 31.0 34.0 30.0 34.0 2 31.709338706281198 31.0 31.0 34.0 30.0 34.0 3 31.755280776447325 31.0 31.0 34.0 30.0 34.0 4 35.48471754842033 37.0 35.0 37.0 33.0 37.0 5 35.35229168281134 37.0 35.0 37.0 33.0 37.0 6 35.403538208281574 37.0 35.0 37.0 33.0 37.0 7 35.31897377008887 37.0 35.0 37.0 32.0 37.0 8 35.286817882585744 37.0 35.0 37.0 32.0 37.0 9 36.75345574323822 39.0 37.0 39.0 32.0 39.0 10-11 36.652984788389304 38.0 35.0 39.0 32.0 39.0 12-13 36.50023913688415 38.0 35.0 39.0 32.0 39.0 14-15 37.34376905560892 39.0 36.0 40.0 32.0 41.0 16-17 37.4365060261712 39.0 36.0 40.0 32.0 41.0 18-19 37.570301155919594 39.0 36.5 41.0 32.0 41.0 20-21 37.4995448180094 39.0 36.0 41.0 32.0 41.0 22-23 37.32468565828396 39.0 36.0 40.5 31.5 41.0 24-25 37.15450923710246 39.0 36.0 40.0 31.0 41.0 26-27 36.852217710845544 39.0 36.0 40.0 30.5 41.0 28-29 36.65683700570875 38.5 35.5 40.0 30.0 41.0 30-31 36.32359877918859 38.0 35.0 40.0 30.0 41.0 32-33 36.1645114993139 38.0 35.0 40.0 30.0 41.0 34-35 35.963241803788776 38.0 35.0 40.0 29.0 41.0 36-37 35.772345013076205 38.0 34.0 40.0 29.0 41.0 38-39 35.46913040279105 38.0 34.0 40.0 27.0 41.0 40-41 35.1487173104576 38.0 34.0 40.0 26.5 41.0 42-43 34.78826366270036 38.0 33.0 40.0 25.5 41.0 44-45 34.39068372014018 38.0 33.0 40.0 24.5 41.0 46-47 33.84291035458794 37.0 32.5 40.0 23.5 41.0 48-49 33.28892725776921 37.0 31.5 39.0 22.5 40.0 50-51 32.753043222328415 36.5 31.0 39.0 19.5 40.0 52-53 31.94702315674777 35.5 30.0 39.0 16.0 40.0 54-55 31.301813056795595 35.0 29.0 38.5 11.0 40.0 56-57 30.720199082434515 35.0 28.0 38.0 8.0 40.0 58-59 29.959801715993052 34.0 27.5 38.0 6.5 40.0 60-61 29.203234844944582 34.0 26.0 38.0 2.0 40.0 62-63 28.410509381132528 33.0 25.5 37.0 2.0 39.0 64-65 27.49597655127823 32.0 23.5 36.5 2.0 39.0 66-67 26.589659306260376 31.5 21.5 36.0 2.0 39.0 68-69 25.61389197431568 31.0 19.0 35.0 2.0 38.0 70-71 24.591863788257008 30.0 15.0 34.5 2.0 38.0 72-73 23.79693971323926 29.0 9.5 34.0 2.0 37.0 74-75 22.89537917873114 29.0 7.0 34.0 2.0 36.0 76-77 21.9761949212179 27.5 4.5 32.5 2.0 35.0 78-79 22.26716227883752 29.0 2.0 34.0 2.0 35.0 80-81 21.696617680787092 29.0 2.0 34.0 2.0 35.0 82-83 20.78401849377422 27.5 2.0 33.0 2.0 35.0 84-85 19.745655419014007 26.0 2.0 32.5 2.0 35.0 86-87 18.56795851464293 24.5 2.0 32.0 2.0 34.0 88-89 17.338295912911903 20.5 2.0 31.0 2.0 34.0 90-91 16.241672283056662 15.0 2.0 31.0 2.0 34.0 92-93 14.945323758464703 4.5 2.0 30.5 2.0 34.0 94-95 13.613540118245556 2.0 2.0 29.5 2.0 34.0 96-97 12.189078019680457 2.0 2.0 29.0 2.0 33.5 98-99 10.601410672784814 2.0 2.0 26.0 2.0 33.0 100-101 7.9273317411748305 2.0 2.0 2.0 2.0 29.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.4053315346835795 1101 2 0.34238865947841646 1101 3 0.3732487210393032 1101 4 0.3214611609451552 1101 5 0.3149925089436394 1101 6 0.3370133540593585 1101 7 0.3050317927099613 1101 8 0.45608800627455537 1101 9 0.4871575036797111 1101 10-11 0.5009242588689773 1101 12-13 0.4784465404372824 1101 14-15 0.571430812016267 1101 16-17 0.6272862671075572 1101 18-19 0.5443061858547438 1101 20-21 0.5954188183064346 1101 22-23 0.6099165609730974 1101 24-25 0.5177807718467946 1101 26-27 0.7237232951586563 1101 28-29 0.7307545222603054 1101 30-31 0.8539938308714952 1101 32-33 0.9870782690030282 1101 34-35 0.7839583341034455 1101 36-37 0.9154780058890495 1101 38-39 1.172748823714798 1101 40-41 1.1562749178133132 1101 42-43 1.3580231414015103 1101 44-45 1.5013755396054265 1101 46-47 1.685626248451996 1101 48-49 1.6663456587929275 1101 50-51 1.549388279671291 1101 52-53 1.7165129394267211 1101 54-55 1.6691264092813434 1101 56-57 1.885269907240044 1101 58-59 2.1378673861938147 1101 60-61 2.2919542382643847 1101 62-63 2.2450747492298326 1101 64-65 2.245312048099109 1101 66-67 2.5412799224732936 1101 68-69 2.6462800321819095 1101 70-71 2.801113642109943 1101 72-73 3.010214310739382 1101 74-75 3.115517150734931 1101 76-77 3.060568753787198 1101 78-79 3.228982012576001 1101 80-81 3.3506237054064805 1101 82-83 3.8979054994414035 1101 84-85 3.981170537432618 1101 86-87 3.9381260514748284 1101 88-89 3.963457107899089 1101 90-91 4.139269215815123 1101 92-93 4.302805685343344 1101 94-95 4.18100956731873 1101 96-97 4.142172582894824 1101 98-99 3.3915972336199136 1101 100-101 2.5449264423156404 1102 1 0.3075871595372668 1102 2 0.35660915022465645 1102 3 0.34392843963085085 1102 4 0.35254545492740874 1102 5 0.29779690463131203 1102 6 0.32151356177332957 1102 7 0.42831145419708605 1102 8 0.3288012919847816 1102 9 0.36844971460885034 1102 10-11 0.42247969143500796 1102 12-13 0.47845019403803946 1102 14-15 0.715282478531833 1102 16-17 0.48152471462493196 1102 18-19 0.5978400296998458 1102 20-21 0.5596721238002331 1102 22-23 0.5452483011560716 1102 24-25 0.5935705923738155 1102 26-27 0.6726374314812702 1102 28-29 0.6157054760530798 1102 30-31 0.6790122003641983 1102 32-33 0.688120560108409 1102 34-35 0.7909425979096625 1102 36-37 0.9860125276800673 1102 38-39 1.1541164070744117 1102 40-41 1.130827453209264 1102 42-43 1.0593124565137444 1102 44-45 1.0694298077553626 1102 46-47 0.9461821099778192 1102 48-49 1.218898849130646 1102 50-51 1.3949235118846843 1102 52-53 1.48464623360789 1102 54-55 1.4789969500277884 1102 56-57 1.70037950849688 1102 58-59 1.8791715457506193 1102 60-61 2.085615917540487 1102 62-63 2.4288632490478292 1102 64-65 2.5632684809398363 1102 66-67 2.5890659080726977 1102 68-69 2.7090238592881093 1102 70-71 3.0048286098297012 1102 72-73 3.179372507592685 1102 74-75 3.143455897441619 1102 76-77 3.254697146730603 1102 78-79 3.216569106975573 1102 80-81 3.4973019057239405 1102 82-83 3.668496922681282 1102 84-85 3.638647022452176 1102 86-87 3.589576463316824 1102 88-89 3.4068675006341103 1102 90-91 3.4787308103818084 1102 92-93 3.559804834189684 1102 94-95 3.4552840231013686 1102 96-97 3.1993397973760747 1102 98-99 2.6008415201325814 1102 100-101 1.8713795943582063 1103 1 0.24626244292695532 1103 2 0.24056984201104115 1103 3 0.2732938124039421 1103 4 0.14780060953272312 1103 5 0.22047279819050658 1103 6 0.277975581268187 1103 7 0.23084212955767924 1103 8 0.2902186599934069 1103 9 0.4079422390940479 1103 10-11 0.4168680621832266 1103 12-13 0.43522815587348873 1103 14-15 0.6386180709579037 1103 16-17 0.5405757053327136 1103 18-19 0.5625652751416013 1103 20-21 0.5032396208532717 1103 22-23 0.5378585076619942 1103 24-25 0.4443402477540417 1103 26-27 0.5999800762443854 1103 28-29 0.5782123558751593 1103 30-31 0.5150938880137161 1103 32-33 0.6272709634073905 1103 34-35 0.7573687310729937 1103 36-37 0.827387489443943 1103 38-39 0.6664156389203271 1103 40-41 0.7739884744228434 1103 42-43 0.7675588062872478 1103 44-45 1.0364424516150166 1103 46-47 1.1922052176011633 1103 48-49 1.1902284556758715 1103 50-51 1.174622427010803 1103 52-53 1.2389038146825584 1103 54-55 1.5560248186354464 1103 56-57 1.8859385307350962 1103 58-59 1.824458763744932 1103 60-61 1.8179788418609668 1103 62-63 1.9989407212731862 1103 64-65 2.326057108409639 1103 66-67 2.3002379080480324 1103 68-69 2.383876430699523 1103 70-71 2.7467977496220577 1103 72-73 2.6470092615659553 1103 74-75 2.5813370355632443 1103 76-77 2.6056291967419867 1103 78-79 2.8987221313761893 1103 80-81 2.8907119811881863 1103 82-83 3.148461526951774 1103 84-85 3.044267592456663 1103 86-87 3.0568665788120484 1103 88-89 2.8979234638480698 1103 90-91 3.195223295457989 1103 92-93 3.2828447115552635 1103 94-95 3.0052297217682433 1103 96-97 3.229892105336882 1103 98-99 2.884728514380205 1103 100-101 2.114746480759173 1104 1 0.2414210863617825 1104 2 0.2581531974438498 1104 3 0.33744605282877416 1104 4 0.27742785137846937 1104 5 0.22592652509848676 1104 6 0.23948631545442822 1104 7 0.23857611167256465 1104 8 0.3047631292037991 1104 9 0.2606830165567615 1104 10-11 0.3864665625237933 1104 12-13 0.3358583982441061 1104 14-15 0.5126874393157834 1104 16-17 0.5178024695475543 1104 18-19 0.4557257668494543 1104 20-21 0.5326842036466175 1104 22-23 0.5529286172022552 1104 24-25 0.4967897740298355 1104 26-27 0.6890405733792804 1104 28-29 0.5557515919043468 1104 30-31 0.6040709267559876 1104 32-33 0.8826113703687426 1104 34-35 0.837155660064596 1104 36-37 0.7899408441185045 1104 38-39 0.886913225426774 1104 40-41 0.9439207313546021 1104 42-43 1.0186005607584363 1104 44-45 1.0027262232364222 1104 46-47 1.2458251500056363 1104 48-49 1.368909153616407 1104 50-51 1.489099038966998 1104 52-53 1.6619646707975875 1104 54-55 1.5963714666936504 1104 56-57 1.7692102613824687 1104 58-59 2.012288130912207 1104 60-61 1.9794272741325933 1104 62-63 2.1597905111774267 1104 64-65 2.4234976364465872 1104 66-67 2.462824158544066 1104 68-69 2.73793541454965 1104 70-71 2.822367303435911 1104 72-73 2.9344056458225083 1104 74-75 2.9464979101148217 1104 76-77 3.06826985877424 1104 78-79 3.1044475182388744 1104 80-81 3.536230509272091 1104 82-83 3.462540733264383 1104 84-85 3.7062252428071787 1104 86-87 3.475349796618694 1104 88-89 3.277749344366189 1104 90-91 3.45550468303043 1104 92-93 3.4486560098843135 1104 94-95 3.1388302502568077 1104 96-97 2.9683945089514214 1104 98-99 2.71258855523312 1104 100-101 2.0334007209138107 1105 1 0.21228337286580157 1105 2 0.26709888244672086 1105 3 0.20604600039930787 1105 4 0.22990937667493938 1105 5 0.1529077240103831 1105 6 0.1019246866951562 1105 7 0.11238298854723894 1105 8 0.17459323842195573 1105 9 0.2880283997560156 1105 10-11 0.36308959242715844 1105 12-13 0.37737551839595795 1105 14-15 0.5468583073947855 1105 16-17 0.44369627685514956 1105 18-19 0.46544786839479 1105 20-21 0.4747479715430032 1105 22-23 0.46873717835499207 1105 24-25 0.41997800547103026 1105 26-27 0.3410741460613025 1105 28-29 0.5082574382355105 1105 30-31 0.6885198715201284 1105 32-33 0.7221229891396703 1105 34-35 0.7876463879950109 1105 36-37 0.8876239592308934 1105 38-39 0.8819931358065531 1105 40-41 0.9179048361660094 1105 42-43 0.9757387287421508 1105 44-45 1.0066315130429047 1105 46-47 1.1738122372071231 1105 48-49 1.2153489254516785 1105 50-51 1.2895911778887523 1105 52-53 1.3053466387740258 1105 54-55 1.3737744034864399 1105 56-57 1.5657844858385062 1105 58-59 1.816678690340563 1105 60-61 1.893025962950336 1105 62-63 2.0194033722890943 1105 64-65 2.340498485269265 1105 66-67 2.4569835827407864 1105 68-69 2.5181348990179764 1105 70-71 2.5592547382611066 1105 72-73 2.7335820353333453 1105 74-75 2.675283655537825 1105 76-77 2.659663108740265 1105 78-79 2.9565039638329047 1105 80-81 3.2140037198937357 1105 82-83 3.281865336274546 1105 84-85 3.5091739918891065 1105 86-87 3.593296046760724 1105 88-89 3.42802363365357 1105 90-91 3.4062037178380997 1105 92-93 3.220194606809393 1105 94-95 3.243967484015739 1105 96-97 3.079966048810812 1105 98-99 2.93394933011985 1105 100-101 2.0785996001411213 1106 1 7.078460777556472E-4 1106 2 0.02710600640510563 1106 3 0.09033978519840957 1106 4 0.08500851591361425 1106 5 0.09394715783681562 1106 6 0.009285305392829457 1106 7 0.08142018255284711 1106 8 -0.0019269795832457248 1106 9 -0.11703579888168036 1106 10-11 0.11146443942052997 1106 12-13 0.02450186149605571 1106 14-15 0.14287364503569222 1106 16-17 -0.09103679355582273 1106 18-19 0.10551892686105901 1106 20-21 0.06284432006153651 1106 22-23 0.12691207153334716 1106 24-25 0.17734383129930364 1106 26-27 -0.018325026533197786 1106 28-29 0.21251694653144426 1106 30-31 0.26071673728029765 1106 32-33 0.1820594017738344 1106 34-35 0.036134810807979534 1106 36-37 0.1831806920507617 1106 38-39 0.2695409999019489 1106 40-41 0.3280754187458186 1106 42-43 0.39032782576533265 1106 44-45 0.5427618301647072 1106 46-47 0.06068958890578102 1106 48-49 0.40789238989905385 1106 50-51 0.6162899476475445 1106 52-53 0.7316498930042599 1106 54-55 0.748918731562835 1106 56-57 0.9151112566779744 1106 58-59 1.2384611741569564 1106 60-61 1.3974980580129177 1106 62-63 1.5564045631372245 1106 64-65 1.9039111481052977 1106 66-67 1.8661368882121963 1106 68-69 1.881772600454692 1106 70-71 1.9729854133920064 1106 72-73 2.2576419973288395 1106 74-75 2.3197943588325067 1106 76-77 2.1242727324232042 1106 78-79 2.0896808568512775 1106 80-81 2.4021328205090846 1106 82-83 2.785044327238289 1106 84-85 2.9214855352675215 1106 86-87 2.7224442054740656 1106 88-89 2.5203207781838977 1106 90-91 2.4544383496335946 1106 92-93 2.337600686021169 1106 94-95 1.9750422055851846 1106 96-97 1.9846412219667702 1106 98-99 1.874201279775198 1106 100-101 1.4063616810614405 1107 1 0.03199863435074235 1107 2 0.06587607094414238 1107 3 0.06444894110543586 1107 4 0.03720155677128645 1107 5 0.1356959182494606 1107 6 0.014686001863758236 1107 7 -7.303613910991658E-4 1107 8 0.07008907731402303 1107 9 0.15525740198827265 1107 10-11 0.18336182242612153 1107 12-13 0.21412788263693727 1107 14-15 0.3296033461580379 1107 16-17 0.326607475153466 1107 18-19 0.14255947458902085 1107 20-21 0.3206429438205376 1107 22-23 0.3540219278232186 1107 24-25 0.3780328545473495 1107 26-27 0.4612566696270406 1107 28-29 0.4601308678252636 1107 30-31 0.3822529078035828 1107 32-33 0.4402947304083611 1107 34-35 0.43431750164725713 1107 36-37 0.4169297037285702 1107 38-39 0.6544613661472596 1107 40-41 0.6679563160483255 1107 42-43 0.6466387942420866 1107 44-45 0.705036622475987 1107 46-47 0.7476573901023826 1107 48-49 0.8595227171531192 1107 50-51 1.082704484014414 1107 52-53 0.9707219271161378 1107 54-55 1.2227636452863457 1107 56-57 1.1261008351919486 1107 58-59 1.1854696141087153 1107 60-61 1.3806948881599261 1107 62-63 1.6057860671623558 1107 64-65 1.743121001912602 1107 66-67 2.045897554380847 1107 68-69 2.2192711805055936 1107 70-71 2.0879153412548277 1107 72-73 2.5151972549771955 1107 74-75 2.3049379297919685 1107 76-77 2.5258986587754286 1107 78-79 2.6960497141138546 1107 80-81 2.7187075572260717 1107 82-83 2.9771433166859076 1107 84-85 3.0368559294604367 1107 86-87 3.2292949426815447 1107 88-89 3.2239048049413803 1107 90-91 3.1857356452229872 1107 92-93 3.114433022192898 1107 94-95 2.8221473525339302 1107 96-97 3.037496241214132 1107 98-99 2.707552856163195 1107 100-101 2.124812104714067 1108 1 -0.017126177305051726 1108 2 -0.009897425504568957 1108 3 -0.014396762604910407 1108 4 0.027612074830578592 1108 5 -0.044566897116951054 1108 6 0.005629073118207373 1108 7 -0.03369725145787328 1108 8 0.0023002328329226884 1108 9 -0.06685033709614885 1108 10-11 -0.0015298427396217562 1108 12-13 0.05116648338562868 1108 14-15 0.08909851927185741 1108 16-17 -0.05609622522836588 1108 18-19 -0.010304256691107128 1108 20-21 0.02106718770316718 1108 22-23 0.056475026803973094 1108 24-25 -0.027052238508481707 1108 26-27 0.17860132682539387 1108 28-29 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-0.6687569816009287 2316 42-43 -0.8099748438986509 2316 44-45 -0.6114129542198938 2316 46-47 -0.7624411320651134 2316 48-49 -0.34781469639118967 2316 50-51 -0.19579024512070475 2316 52-53 -0.02739669755420948 2316 54-55 0.4120454855041551 2316 56-57 0.2539334896748464 2316 58-59 0.22177702317474868 2316 60-61 0.4647192272581542 2316 62-63 0.49010888719946166 2316 64-65 0.7292354895049193 2316 66-67 0.7119917830859137 2316 68-69 1.059701664088788 2316 70-71 0.9276106760240559 2316 72-73 1.1595173738293347 2316 74-75 1.2650198183467651 2316 76-77 1.3504135472804641 2316 78-79 1.3038885528886546 2316 80-81 0.7249586679106805 2316 82-83 1.093364216749169 2316 84-85 1.2555170658063055 2316 86-87 1.4009574509450395 2316 88-89 1.3535503810401543 2316 90-91 1.4708794833224879 2316 92-93 1.3237003613124507 2316 94-95 1.7512140220439498 2316 96-97 1.4665418699149324 2316 98-99 1.257011552912532 2316 100-101 0.8613182641266324 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 10.0 3 26.0 4 82.0 5 172.0 6 335.0 7 913.0 8 2062.0 9 3509.0 10 5662.0 11 8849.0 12 13154.0 13 18491.0 14 24983.0 15 33101.0 16 41059.0 17 50062.0 18 52870.0 19 53751.0 20 58442.0 21 63898.0 22 69839.0 23 74764.0 24 80804.0 25 88184.0 26 96794.0 27 109540.0 28 123753.0 29 138494.0 30 152149.0 31 166092.0 32 177591.0 33 191481.0 34 199683.0 35 195313.0 36 162256.0 37 83947.0 38 12821.0 39 86.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 40.88492976934279 20.476581951425754 15.883324720878159 22.755163558353296 2 17.010465847237302 21.274345483339896 38.260817148677035 23.454371520745767 3 20.158028510572677 24.08287025114071 30.04154236703037 25.717558871256234 4 14.531264310052908 17.66352696767386 35.6033333568165 32.201875365456736 5 14.1950076281263 36.9519118847695 34.219289257453376 14.633791229650829 6 33.00507784277396 36.31369906012551 16.534926118052994 14.146296979047538 7 29.042489653709442 32.113852640016404 20.237751377483246 18.605906328790905 8 26.226271241500072 36.050139685685686 19.00058003414452 18.723009038669723 9 25.967018627657417 16.90868714036457 19.8033047125304 37.320989519447615 10-11 25.369409735023808 26.62104279336929 26.988397751565348 21.02114972004155 12-13 25.962731436363367 23.594904466576022 27.564028802883104 22.878335294177507 14-15 22.67056800293696 25.32238469962294 25.62680477898038 26.380242518459724 16-17 22.503230891945353 28.466741186572953 26.40084899464662 22.629178926835074 18-19 22.402996921357232 27.02755592711139 28.65799198597899 21.91145516555239 20-21 22.910370243387337 26.161555556077403 28.92951606678925 21.99855813374601 22-23 22.707867094686467 25.977486690916944 28.81337225276338 22.501273961633206 24-25 22.608296431515264 26.31243791531669 28.84302721980385 22.2362384333642 26-27 22.367425510087056 26.631208047255196 28.658052324068063 22.34331411858969 28-29 22.385746133481955 26.699855030035085 28.74514875076908 22.16925008571388 30-31 22.509688761975436 26.429302761385227 28.72601488362918 22.334993593010157 32-33 22.404502047052162 26.390583540491242 28.703681053989694 22.501233358466905 34-35 22.87977100207728 26.514698117217783 28.53295172231849 22.072579158386446 36-37 22.91897750053425 26.265267159140098 28.496524845068127 22.31923049525752 38-39 22.50097123861547 26.53183167284965 28.437220799478613 22.52997628905627 40-41 22.885792683404183 26.428932102679724 28.40157902565427 22.28369618826182 42-43 22.703314247736746 26.44775604149449 28.297167768441227 22.551761942327538 44-45 22.725114755022293 26.578527347076804 28.319735202394668 22.37662269550624 46-47 22.845187678606756 26.801008759424338 27.746950659280685 22.606852902688217 48-49 22.86217104979918 26.755992707694887 27.78909535808302 22.592740884422913 50-51 22.95821718951931 26.994659928564218 27.610955991768364 22.436166890148108 52-53 23.048079429453054 26.83432862809009 27.529273720539393 22.588318221917465 54-55 22.90481256130084 26.892566874179558 27.559253892921472 22.64336667159813 56-57 22.893858231721335 27.050437736042532 27.502880112750518 22.55282391948561 58-59 22.96551653958361 26.779593287259367 27.483559828447664 22.771330344709362 60-61 22.901348794648342 26.95712600517726 27.588803540634878 22.552721659539525 62-63 22.692720454070454 26.94102046870829 27.709135185528734 22.657123891692518 64-65 22.608407727771834 26.846733203693045 27.61070027477296 22.93415879376216 66-67 22.704374675391517 26.87190729083863 27.443472624863947 22.980245408905905 68-69 22.78943175393597 26.761985891549234 27.47721276003246 22.971369594482336 70-71 22.82951881804494 26.752813381964373 27.36965762708017 23.048010172910516 72-73 22.82989000649507 26.79759743046468 27.200941521075517 23.171571041964732 74-75 22.793776335389676 27.04724264605158 26.885815464602654 23.27316555395609 76-77 22.702348551206214 26.850179763618478 27.363541292403742 23.083930392771567 78-79 22.747123116748114 26.84896646682494 27.374265270514304 23.02964514591264 80-81 22.727663224356302 26.995147001871022 27.22815901333004 23.049030760442633 82-83 22.80292956423466 26.933282452458187 27.18222691891839 23.08156106438877 84-85 22.808616485378906 26.822832472885118 27.176568670812994 23.191982370922982 86-87 22.779677057812677 26.720531461803727 27.181603462032307 23.31818801835129 88-89 22.673444690495817 27.001704877687942 26.869377249980626 23.455473181835615 90-91 22.7314508315406 26.790004565581853 27.069477666516047 23.4090669363615 92-93 22.682820820361265 26.571516298282372 26.999205677789327 23.746457203567033 94-95 22.58131683040632 26.657133804940585 26.884666136860297 23.8768832277928 96-97 22.593082405193538 26.58121009572915 26.795494048776895 24.03021345030041 98-99 22.523837368132252 26.53374413214446 26.675973827231232 24.266444672492057 100-101 22.98808736843053 26.28279867977498 26.713265145973473 24.015848805821012 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 426.0 1 313.0 2 266.5 3 358.0 4 338.5 5 320.0 6 620.0 7 1449.5 8 2238.5 9 2447.5 10 2127.0 11 1723.5 12 1750.0 13 1945.5 14 2156.5 15 2388.5 16 2619.5 17 2953.5 18 3229.0 19 3555.5 20 3994.0 21 4652.5 22 5592.5 23 6900.0 24 8603.0 25 10841.5 26 13941.0 27 17327.5 28 20661.0 29 24132.5 30 28067.5 31 32892.5 32 38116.5 33 41625.5 34 44805.0 35 49423.5 36 54799.5 37 59856.5 38 63599.0 39 66988.5 40 70813.5 41 75982.0 42 80917.5 43 85051.5 44 89053.5 45 92831.0 46 95526.0 47 97214.0 48 97494.5 49 96788.5 50 96436.5 51 94377.0 52 91757.5 53 90478.5 54 89194.5 55 83976.5 56 75850.0 57 68771.0 58 62295.0 59 54812.5 60 46117.5 61 38165.5 62 31719.5 63 25548.5 64 20653.0 65 16979.0 66 13363.5 67 10444.0 68 7884.0 69 5851.5 70 4476.0 71 3430.0 72 2702.5 73 2180.5 74 1784.5 75 1439.5 76 1063.5 77 674.5 78 406.0 79 257.0 80 165.0 81 104.5 82 67.0 83 38.5 84 22.0 85 13.0 86 6.5 87 5.0 88 2.5 89 1.5 90 1.5 91 1.0 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02277866883337991 2 0.009236711073329311 3 0.001565544249716832 4 0.0 5 1.5655442497168322E-4 6 0.0 7 0.0 8 0.0 9 1.5655442497168322E-4 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0017220986746885154 26-27 0.008336523129742132 28-29 3.91386062429208E-4 30-31 0.0 32-33 2.544009405789852E-4 34-35 0.009354126892058072 36-37 0.0010958809748017825 38-39 0.011526319538540178 40-41 0.011037086960503666 42-43 0.005029310902215323 44-45 5.479404874008913E-4 46-47 9.7846515607302E-5 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 1.9569303121460402E-5 58-59 0.0 60-61 0.0 62-63 0.0 64-65 1.3698512185022281E-4 66-67 8.219107311013369E-4 68-69 0.0011154502779232428 70-71 3.9138606242920804E-5 72-73 2.9353954682190606E-4 74-75 0.0 76-77 0.0 78-79 0.0 80-81 1.3698512185022281E-4 82-83 0.0013502819153807678 84-85 0.005949068148923962 86-87 0.004168261564871066 88-89 0.0 90-91 0.0013894205216236884 92-93 5.283711842794309E-4 94-95 1.9569303121460402E-5 96-97 3.9138606242920804E-5 98-99 0.0 100-101 1.1741581872876241E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2555022.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.27151052450085 #Duplication Level Percentage of deduplicated Percentage of total 1 76.83941957739843 43.23870207446112 2 13.274824500393335 14.939888531695702 3 4.308023134553712 7.27256907467497 4 1.8795031711803343 4.230499299116276 5 0.9897457483180846 2.7847244146530534 6 0.6091653397256771 2.0567192295320744 7 0.38462031867097757 1.515021641702155 8 0.2745147405127226 1.2357887287913834 9 0.19652086122576382 0.9952673139674585 >10 1.102507298694754 11.45396671122145 >50 0.09191295775872774 3.5434514395056063 >100 0.04589189528584328 4.628484188014764 >500 0.0023034386936801035 0.7958936427934417 >1k 9.074152429537677E-4 0.7554371234116439 >5k 1.396023450698104E-4 0.5535865864588837 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7536 0.2949485366466512 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6558 0.2566709797410746 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4733 0.18524302334774417 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 3.9138606242920804E-5 0.0 5 0.0 0.0 0.0 3.9138606242920804E-5 0.0 6 3.9138606242920804E-5 0.0 0.0 3.9138606242920804E-5 0.0 7 3.9138606242920804E-5 0.0 0.0 3.9138606242920804E-5 0.0 8 3.9138606242920804E-5 0.0 0.0 3.9138606242920804E-5 0.0 9 3.9138606242920804E-5 0.0 0.0 1.1741581872876241E-4 0.0 10-11 7.827721248584161E-5 0.0 0.0 1.1741581872876241E-4 0.0 12-13 7.827721248584161E-5 0.0 0.0 1.1741581872876241E-4 0.0 14-15 9.784651560730201E-5 0.0 0.0 1.1741581872876241E-4 0.0 16-17 1.1741581872876241E-4 0.0 0.0 1.1741581872876241E-4 0.0 18-19 1.1741581872876241E-4 0.0 0.0 1.1741581872876241E-4 0.0 20-21 1.1741581872876241E-4 0.0 0.0 2.1526233433606442E-4 0.0 22-23 1.1741581872876241E-4 0.0 0.0 2.93539546821906E-4 0.0 24-25 1.1741581872876241E-4 0.0 0.0 4.1095536555066847E-4 0.0 26-27 1.1741581872876241E-4 0.0 0.0 6.262176998867329E-4 0.0 28-29 1.1741581872876241E-4 0.0 0.0 0.0014676977341095301 0.0 30-31 1.1741581872876241E-4 0.0 0.0 0.005420696964644531 0.0 32-33 1.1741581872876241E-4 0.0 0.0 0.014500853613002157 0.0 34-35 1.3698512185022281E-4 0.0 0.0 0.029432231894676447 0.0 36-37 1.5655442497168322E-4 0.0 0.0 0.054774479436967664 0.0 38-39 1.5655442497168322E-4 0.0 0.0 0.0947937043203542 0.0 40-41 1.9569303121460402E-4 0.0 0.0 0.13950956195289121 0.0 42-43 1.9569303121460402E-4 0.0 0.0 0.18700426062867562 0.0 44-45 2.3483163745752482E-4 0.0 0.0 0.24686675887722298 0.0 46-47 2.7397024370044563E-4 0.0 0.0 0.31950801206408397 0.0 48-49 2.7397024370044563E-4 0.0 0.0 0.3977069473374397 0.0 50-51 2.7397024370044563E-4 0.0 0.0 0.4834009257063149 0.0 52-53 2.7397024370044563E-4 0.0 0.0 0.5684295477690603 0.0 54-55 2.7397024370044563E-4 0.0 0.0 0.6666283108325486 0.0 56-57 2.7397024370044563E-4 0.0 0.0 0.7822828922803795 0.0 58-59 2.7397024370044563E-4 0.0 0.0 0.9068023680422321 0.0 60-61 2.7397024370044563E-4 0.0 0.0 1.042554623795803 0.0 62-63 2.7397024370044563E-4 0.0 0.0 1.1806160573177062 0.0 64-65 3.1310884994336643E-4 0.0 0.0 1.3287165433409185 0.0 66-67 3.1310884994336643E-4 0.0 0.0 1.4936074914423436 0.0 68-69 3.1310884994336643E-4 0.0 0.0 1.687695839800988 0.0 70-71 3.1310884994336643E-4 0.0 0.0 1.8896901866207023 0.0 72-73 3.1310884994336643E-4 0.0 0.0 2.0977510174080694 0.0 74-75 3.1310884994336643E-4 0.0 0.0 2.3151659750874947 0.0 76-77 3.1310884994336643E-4 0.0 0.0 2.5523067903133514 0.0 78-79 3.5224745618628724E-4 0.0 0.0 2.8152986549626577 0.0 80-81 3.5224745618628724E-4 0.0 0.0 3.097409728761631 0.0 82-83 3.5224745618628724E-4 0.0 0.0 3.388346558268383 1.9569303121460402E-5 84-85 3.5224745618628724E-4 0.0 0.0 3.6727668098356885 3.9138606242920804E-5 86-87 3.9138606242920804E-4 0.0 0.0 3.9739579541780854 3.9138606242920804E-5 88-89 3.9138606242920804E-4 0.0 0.0 4.292976733664133 3.9138606242920804E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3925 0.0 57.5004 1 GTATCAA 7135 0.0 43.551254 1 ATCAACG 9400 0.0 32.439907 3 TATCAAC 9595 0.0 31.882126 2 TCAACGC 9675 0.0 31.468748 4 CAACGCA 9790 0.0 31.099094 5 AACGCAG 10320 0.0 29.594 6 TGGTATC 1695 0.0 27.183693 2 CGCAGAG 11820 0.0 25.597311 8 ACGCAGA 11955 0.0 25.467178 7 GTGGTAT 2090 0.0 23.415737 1 GCAGAGT 13015 0.0 22.626633 9 GAGTACT 8395 0.0 21.697868 12-13 TAAGGTG 1125 0.0 21.11008 5 TACATGG 7250 0.0 20.573019 2 AGAGTAC 12085 0.0 20.41791 10-11 GTACATG 7425 0.0 20.34921 1 GTACTTT 8845 0.0 20.191214 14-15 AGTACTT 8930 0.0 20.105402 12-13 CAGAGTA 12525 0.0 20.09882 10-11 >>END_MODULE