FastQCFastQC Report
Thu 26 May 2016
SRR938291_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938291_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2959841
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT154050.5204671467149755No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT121170.4093800984579915No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77370.26139917650981925No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC38010.1284190603481741No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA37640.12716899319929686No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA35380.1195334479115601No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA34860.1177765967834083No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA33890.11449939371743278No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31640.10689763402831436No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA31160.10527592529463575No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA74350.052.420641
GTATCAA130400.041.5952841
TATCAAC165600.032.516572
ATCAACG166100.032.3900763
TCAACGC168250.031.9196744
CAACGCA171150.031.2955175
AACGCAG175850.030.47946
ACGCAGA197000.027.008687
CGCAGAG198400.026.7702058
TAAGGTG19350.024.8070745
GCAGAGT214350.024.2224969
GAGTACT129850.023.9068412-13
TGGTATC34950.023.7972072
AGGTAAG18100.023.6319242
GTAAGGT20000.023.2879474
GTACTTT135350.022.75931714-15
GTGGTAT38900.022.5646951
CAGAGTA202300.021.80259710-11
AGTACTT132550.021.6817412-13
TACATGG108300.021.590952
Produced by FastQC (version 0.11.5)