##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938291_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2959841 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.789221785899986 33.0 31.0 34.0 30.0 34.0 2 31.964315650739348 33.0 31.0 34.0 30.0 34.0 3 32.047782972125866 34.0 31.0 34.0 30.0 34.0 4 35.58722444888087 37.0 35.0 37.0 33.0 37.0 5 35.58969924397966 37.0 35.0 37.0 33.0 37.0 6 35.624732544754934 37.0 35.0 37.0 33.0 37.0 7 35.55098398866696 37.0 35.0 37.0 33.0 37.0 8 35.51693351095549 37.0 35.0 37.0 33.0 37.0 9 37.12677201241553 39.0 37.0 39.0 34.0 39.0 10-11 37.01478525366734 39.0 37.0 39.0 33.0 39.0 12-13 36.87129105921568 39.0 37.0 39.0 33.0 39.0 14-15 37.97697562132561 40.0 38.0 41.0 33.0 41.0 16-17 37.7799001703132 40.0 38.0 41.0 32.5 41.0 18-19 37.98686855138502 40.0 38.0 41.0 33.0 41.0 20-21 37.96909614401584 40.0 38.0 41.0 33.0 41.0 22-23 37.84772307025952 40.0 38.0 41.0 32.5 41.0 24-25 37.69001814624502 40.0 38.0 41.0 32.0 41.0 26-27 37.45881805813218 40.0 37.0 41.0 32.0 41.0 28-29 37.22035389738841 40.0 37.0 41.0 31.0 41.0 30-31 36.92323675494731 39.5 36.5 41.0 30.5 41.0 32-33 36.55608088407452 39.0 35.5 41.0 30.0 41.0 34-35 36.21715676619115 39.0 35.0 40.5 30.0 41.0 36-37 35.861688178520396 38.5 35.0 40.0 28.5 41.0 38-39 35.473792849007765 38.0 35.0 40.0 27.0 41.0 40-41 35.24517026421351 38.0 35.0 40.0 26.0 41.0 42-43 34.91728440818274 38.0 34.0 40.0 24.5 41.0 44-45 34.482821881310514 38.0 33.5 40.0 23.5 41.0 46-47 33.94744869741314 38.0 33.0 40.0 21.5 41.0 48-49 33.37977259589282 38.0 32.5 40.0 16.5 41.0 50-51 32.49773045241281 37.0 31.5 39.5 13.5 40.5 52-53 32.438831680485535 37.0 31.0 39.5 10.5 40.5 54-55 32.34219878702944 37.0 31.0 40.0 7.5 41.0 56-57 31.79549661620337 36.5 30.5 40.0 3.5 41.0 58-59 31.25007458170895 36.0 30.0 39.0 2.0 41.0 60-61 30.60819077781543 35.0 28.5 39.0 2.0 40.5 62-63 29.914325465455747 35.0 28.0 39.0 2.0 40.0 64-65 29.233339392217353 34.5 26.5 38.0 2.0 40.0 66-67 28.482900770683287 34.0 26.0 37.5 2.0 40.0 68-69 27.705342111282327 34.0 24.0 37.0 2.0 39.5 70-71 26.792847656343703 33.0 21.0 36.0 2.0 39.0 72-73 25.930897470506018 32.5 18.0 36.0 2.0 39.0 74-75 25.048970704845296 32.0 10.0 35.0 2.0 38.0 76-77 24.16093769901829 31.0 4.5 35.0 2.0 37.0 78-79 23.284471023950275 30.5 2.0 35.0 2.0 36.5 80-81 22.4593920754527 30.0 2.0 34.0 2.0 36.0 82-83 21.602059198450185 29.0 2.0 34.0 2.0 35.5 84-85 20.76886815879637 28.5 2.0 34.0 2.0 35.0 86-87 19.985893836864886 26.5 2.0 34.0 2.0 35.0 88-89 19.116559977377165 25.5 2.0 34.0 2.0 35.0 90-91 18.243222693381163 24.5 2.0 33.0 2.0 35.0 92-93 17.33826073765449 19.5 2.0 33.0 2.0 35.0 94-95 16.41078372115259 12.0 2.0 32.0 2.0 34.5 96-97 15.328369159019015 2.0 2.0 32.0 2.0 34.0 98-99 14.053399827896161 2.0 2.0 31.0 2.0 34.0 100-101 11.301941894851783 2.0 2.0 26.5 2.0 32.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.5646921138046217 1101 2 0.4788157895428782 1101 3 0.554784114345388 1101 4 0.4191967184187817 1101 5 0.36458718225358666 1101 6 0.3860953094582129 1101 7 0.3817230959480469 1101 8 0.41526628639189056 1101 9 0.5425100171590245 1101 10-11 0.6265355978964564 1101 12-13 0.5679174508103841 1101 14-15 0.7830769472815078 1101 16-17 0.8924557928590815 1101 18-19 0.8658017333545445 1101 20-21 0.8805523855094037 1101 22-23 0.9115240357258116 1101 24-25 0.9819055245066792 1101 26-27 1.051057971944637 1101 28-29 1.1231871754025846 1101 30-31 1.2273217534057537 1101 32-33 1.346538871023533 1101 34-35 1.3714123474630995 1101 36-37 1.3935536003755615 1101 38-39 1.498911635566543 1101 40-41 1.615361001507381 1101 42-43 1.9649489432759424 1101 44-45 2.0654463648144414 1101 46-47 2.0945372622128957 1101 48-49 2.280993473277519 1101 50-51 2.553372603388489 1101 52-53 2.6245890339247566 1101 54-55 2.687728537887601 1101 56-57 2.899461775502104 1101 58-59 3.2223398385876685 1101 60-61 3.5171306924028016 1101 62-63 3.6435072380278832 1101 64-65 3.681918699379633 1101 66-67 3.7866367770883436 1101 68-69 4.166467337669317 1101 70-71 4.505805341303013 1101 72-73 4.866455409766729 1101 74-75 4.929056590143041 1101 76-77 5.205756573583468 1101 78-79 5.497322611513642 1101 80-81 5.514203062035872 1101 82-83 5.823518709165899 1101 84-85 5.949136849153955 1101 86-87 6.118404936028885 1101 88-89 6.263755301330971 1101 90-91 6.202626474626893 1101 92-93 6.557486552585612 1101 94-95 6.456039667225955 1101 96-97 6.501205061827774 1101 98-99 6.37114259868749 1101 100-101 5.227358038379444 1102 1 0.5788739504786555 1102 2 0.47142085512980714 1102 3 0.4566341429781744 1102 4 0.3331636433648697 1102 5 0.3085869472531044 1102 6 0.4245992218687391 1102 7 0.44912669758307544 1102 8 0.5293726007347246 1102 9 0.5487688000650124 1102 10-11 0.5322144128875976 1102 12-13 0.5840516234491915 1102 14-15 0.7700797467562879 1102 16-17 0.8206107927311592 1102 18-19 0.7404287411635764 1102 20-21 0.727217258086398 1102 22-23 0.7967627838934206 1102 24-25 0.7628645475616764 1102 26-27 0.9464688611213816 1102 28-29 1.0425670078763076 1102 30-31 1.0031421151232962 1102 32-33 1.1306108607669003 1102 34-35 1.0803678082393375 1102 36-37 1.197069036969502 1102 38-39 1.3046298068206141 1102 40-41 1.430079706882161 1102 42-43 1.544684384538293 1102 44-45 1.591320896669167 1102 46-47 1.7167203755965232 1102 48-49 1.9296062439742272 1102 50-51 2.3125277513993083 1102 52-53 2.3673283403764316 1102 54-55 2.431058820005049 1102 56-57 2.7326806882395402 1102 58-59 2.8431160287434842 1102 60-61 2.941902664552714 1102 62-63 3.1321464336439853 1102 64-65 3.1320626687588167 1102 66-67 3.3269627328596165 1102 68-69 3.641376456603883 1102 70-71 3.764319528667251 1102 72-73 3.951078324019033 1102 74-75 4.372258885440726 1102 76-77 4.561978369182956 1102 78-79 4.854427475107549 1102 80-81 4.976127299018689 1102 82-83 5.300135766726168 1102 84-85 5.368957149594483 1102 86-87 5.621576336976023 1102 88-89 5.5475305397980215 1102 90-91 5.6338743050576205 1102 92-93 5.854321137201737 1102 94-95 5.866692422116984 1102 96-97 5.654228513548913 1102 98-99 5.5066908115309605 1102 100-101 4.728229498487325 1103 1 0.5032207145453746 1103 2 0.5092761426568302 1103 3 0.4155105255677256 1103 4 0.33697956185157807 1103 5 0.42098309177062987 1103 6 0.40304831333036617 1103 7 0.4178854776359415 1103 8 0.3802851528416511 1103 9 0.5771860683146244 1103 10-11 0.4411957260681376 1103 12-13 0.6198197669804841 1103 14-15 0.7569204185414335 1103 16-17 0.8901903822977317 1103 18-19 0.8173592060118295 1103 20-21 0.8021852915265697 1103 22-23 0.9202712500146006 1103 24-25 0.8820553778894862 1103 26-27 1.018810762830661 1103 28-29 1.1067782222615463 1103 30-31 1.1502671316082882 1103 32-33 1.2812177953601704 1103 34-35 1.2635906682000027 1103 36-37 1.199804113029849 1103 38-39 1.5564761265091462 1103 40-41 1.617257532697387 1103 42-43 1.7321259703090206 1103 44-45 1.7560131862775918 1103 46-47 1.9525633466803995 1103 48-49 1.9733803622418904 1103 50-51 2.134988631784175 1103 52-53 2.234993950091976 1103 54-55 2.3430590378937026 1103 56-57 2.5286838648268315 1103 58-59 2.736430860653858 1103 60-61 3.0232511086556713 1103 62-63 3.100103899484207 1103 64-65 3.2451913205440768 1103 66-67 3.5231514015025702 1103 68-69 3.7537640002532875 1103 70-71 4.115574682020728 1103 72-73 4.346772729441021 1103 74-75 4.449229487673513 1103 76-77 4.863414427462438 1103 78-79 4.849513512458252 1103 80-81 5.012411494636833 1103 82-83 5.365804828256781 1103 84-85 5.380731738042947 1103 86-87 5.587909672220661 1103 88-89 5.814088644396048 1103 90-91 5.804266103269786 1103 92-93 5.958757178279484 1103 94-95 5.890164191200878 1103 96-97 5.569628941298758 1103 98-99 5.525506644008987 1103 100-101 4.440650872217981 1104 1 0.5264701886090499 1104 2 0.3851802773786943 1104 3 0.3550805238515764 1104 4 0.31592028251166226 1104 5 0.24723472892441833 1104 6 0.2829111469113741 1104 7 0.24065788212394779 1104 8 0.31232534925587885 1104 9 0.417229669637841 1104 10-11 0.43254743128302664 1104 12-13 0.46951724392694416 1104 14-15 0.6423717936650277 1104 16-17 0.771549247407961 1104 18-19 0.6642614849242037 1104 20-21 0.6924086463091612 1104 22-23 0.7333847145979462 1104 24-25 0.8047750139916232 1104 26-27 0.8359775784062222 1104 28-29 0.8588147410088709 1104 30-31 0.9223450091294296 1104 32-33 0.9583506674111675 1104 34-35 0.93401422329989 1104 36-37 1.1345648984870564 1104 38-39 1.2051075080208662 1104 40-41 1.3312654349735809 1104 42-43 1.3605151336597672 1104 44-45 1.3788110262973703 1104 46-47 1.5151072935873344 1104 48-49 1.5991052855964867 1104 50-51 1.773262559194329 1104 52-53 1.8148559562692554 1104 54-55 1.9608024397413004 1104 56-57 2.3781908249327017 1104 58-59 2.496860177622093 1104 60-61 2.495135216783652 1104 62-63 2.7867885055950943 1104 64-65 2.965338415807775 1104 66-67 3.0615333764268193 1104 68-69 3.2455294073309275 1104 70-71 3.5191134592235898 1104 72-73 3.753863993070535 1104 74-75 3.952239525364604 1104 76-77 4.111361075328968 1104 78-79 4.349301882557082 1104 80-81 4.621151246064787 1104 82-83 4.924862481035095 1104 84-85 5.0762062248350865 1104 86-87 5.2097077089628065 1104 88-89 5.389258344186871 1104 90-91 5.4212072088152645 1104 92-93 5.464040365050607 1104 94-95 5.44204868164643 1104 96-97 5.325404825590054 1104 98-99 4.943327764744447 1104 100-101 3.849835395945137 1105 1 0.5413719810563187 1105 2 0.4022816962336151 1105 3 0.37700362467067805 1105 4 0.3432302744453182 1105 5 0.344142418829378 1105 6 0.3491844483252109 1105 7 0.3774918255670414 1105 8 0.38678781276127694 1105 9 0.4432363835817199 1105 10-11 0.4811716402299311 1105 12-13 0.560344502323673 1105 14-15 0.7606605589280022 1105 16-17 0.8178399162291541 1105 18-19 0.7348946991253342 1105 20-21 0.75734865572187 1105 22-23 0.7186836285373275 1105 24-25 0.6625058417701766 1105 26-27 0.7709711703819622 1105 28-29 0.7963686529379075 1105 30-31 1.012988045859089 1105 32-33 1.0778110299357664 1105 34-35 1.1570070970409887 1105 36-37 1.2510662210081662 1105 38-39 1.3420368132680878 1105 40-41 1.5801173169440403 1105 42-43 1.533283270102281 1105 44-45 1.6232127564179493 1105 46-47 1.698169250781966 1105 48-49 1.6754174528563652 1105 50-51 1.8495005123779933 1105 52-53 1.9479260638770413 1105 54-55 2.1437334386456897 1105 56-57 2.5471635353003848 1105 58-59 2.611368162596964 1105 60-61 2.8010941079649285 1105 62-63 3.0520833371035287 1105 64-65 3.1544542713364727 1105 66-67 3.2481415186173095 1105 68-69 3.3368541627430055 1105 70-71 3.6657594230089607 1105 72-73 3.9625864194216014 1105 74-75 4.092454543903017 1105 76-77 4.452363656271242 1105 78-79 4.444884884039656 1105 80-81 4.731706773233636 1105 82-83 4.8248484394691715 1105 84-85 4.982278197252146 1105 86-87 4.93119259208089 1105 88-89 4.803617270518039 1105 90-91 4.923998905928308 1105 92-93 4.968390426411858 1105 94-95 4.818263805792132 1105 96-97 4.512690680216371 1105 98-99 4.378861477572228 1105 100-101 3.5615139725480525 1106 1 0.3886020043408429 1106 2 0.27446884035919794 1106 3 0.31149943871795926 1106 4 0.23978197260316136 1106 5 0.24707259200730647 1106 6 0.23273596654064477 1106 7 0.22538511180644605 1106 8 0.18774237397490623 1106 9 0.2948400245713714 1106 10-11 0.2957848098797129 1106 12-13 0.2648730362551248 1106 14-15 0.4874114783232173 1106 16-17 0.48040407172602073 1106 18-19 0.4493809673093452 1106 20-21 0.5128447787979553 1106 22-23 0.5040006542914455 1106 24-25 0.5721755677089675 1106 26-27 0.591823306315959 1106 28-29 0.6400884807205856 1106 30-31 0.7242426965165336 1106 32-33 0.7250194299940773 1106 34-35 0.7897904696087963 1106 36-37 0.9840880643593195 1106 38-39 1.1464558623509262 1106 40-41 1.280185715151454 1106 42-43 1.153953492169805 1106 44-45 1.4695170745712005 1106 46-47 1.5669884706908022 1106 48-49 1.7530455883654312 1106 50-51 1.958163565379131 1106 52-53 2.062235830279647 1106 54-55 2.1852661059786342 1106 56-57 2.073450007385645 1106 58-59 2.54900505789848 1106 60-61 2.9724829758207676 1106 62-63 2.6897962904080295 1106 64-65 2.8230922052309317 1106 66-67 3.101053854517179 1106 68-69 3.0960603266432933 1106 70-71 3.343067303720577 1106 72-73 3.6421362343998673 1106 74-75 3.6527150347150936 1106 76-77 3.554946589568612 1106 78-79 3.900280827035207 1106 80-81 3.9668032282676897 1106 82-83 4.060985057071708 1106 84-85 4.361729401780114 1106 86-87 4.198671523562009 1106 88-89 4.422917770451551 1106 90-91 4.587642401964491 1106 92-93 4.5447971384521395 1106 94-95 4.413767455559157 1106 96-97 4.492340863630803 1106 98-99 4.053184423846043 1106 100-101 3.1682446851513912 1107 1 0.579486372597092 1107 2 0.5375674139829343 1107 3 0.49578939805972055 1107 4 0.400434126579178 1107 5 0.4206985748518619 1107 6 0.40474285666856247 1107 7 0.3901007193436641 1107 8 0.4582394127296965 1107 9 0.4609019646003034 1107 10-11 0.4788948254579921 1107 12-13 0.4816512214263753 1107 14-15 0.6599705020262832 1107 16-17 0.5926737275253231 1107 18-19 0.6546226177707339 1107 20-21 0.6830363036411597 1107 22-23 0.6802904788751789 1107 24-25 0.7195282275890662 1107 26-27 0.7640773775992074 1107 28-29 0.8795441331646714 1107 30-31 0.9935898625115982 1107 32-33 0.9075351377263772 1107 34-35 1.0140575824742584 1107 36-37 1.2154034586544356 1107 38-39 1.411355888741042 1107 40-41 1.4406210709157605 1107 42-43 1.2922391829263447 1107 44-45 1.3858944170056944 1107 46-47 1.7811447388418031 1107 48-49 1.8608229980541608 1107 50-51 1.9502784116582816 1107 52-53 2.0612761160664803 1107 54-55 2.1387086432226923 1107 56-57 2.2070270905357106 1107 58-59 2.493202383575561 1107 60-61 2.584382546154785 1107 62-63 2.772931385952095 1107 64-65 3.0667229857107365 1107 66-67 3.171137198612435 1107 68-69 3.1146992331567844 1107 70-71 3.3788499889364374 1107 72-73 3.603878056740129 1107 74-75 3.8464240185210485 1107 76-77 3.9592328929750558 1107 78-79 4.128037548871557 1107 80-81 4.064262103674533 1107 82-83 4.527922487548107 1107 84-85 4.364160349210135 1107 86-87 4.454785326714227 1107 88-89 4.813274523606665 1107 90-91 4.701021503115985 1107 92-93 4.716675883680239 1107 94-95 4.512688829866416 1107 96-97 4.513423819048979 1107 98-99 4.246516334733558 1107 100-101 3.42341599317143 1108 1 0.4392824909704949 1108 2 0.3681142047022874 1108 3 0.3956711360803098 1108 4 0.22683592355349447 1108 5 0.2799230092455858 1108 6 0.1405459745304256 1108 7 0.2184602965380904 1108 8 0.23382451830446627 1108 9 0.34144748913143275 1108 10-11 0.4285250351894021 1108 12-13 0.3646492892231521 1108 14-15 0.6025418390703905 1108 16-17 0.6277680332236812 1108 18-19 0.5545308191250555 1108 20-21 0.5503732059370066 1108 22-23 0.546066234409686 1108 24-25 0.5380829497114092 1108 26-27 0.69243600515248 1108 28-29 0.6569124749139306 1108 30-31 0.6959967596920507 1108 32-33 0.7262796614623497 1108 34-35 0.9463474712016406 1108 36-37 1.0930348510056476 1108 38-39 1.232039389802111 1108 40-41 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2.690586440738908 2311 66-67 2.716526867349298 2311 68-69 3.015065238710857 2311 70-71 3.3694700724938684 2311 72-73 3.464025112256234 2311 74-75 3.8054959762304037 2311 76-77 3.7711742592601354 2311 78-79 3.9963194058129154 2311 80-81 4.051440021464387 2311 82-83 4.480787879969249 2311 84-85 4.793341889035929 2311 86-87 4.606783443940959 2311 88-89 4.536885817675927 2311 90-91 4.794882623419628 2311 92-93 5.127800631867345 2311 94-95 5.121192796220679 2311 96-97 4.730639253077483 2311 98-99 4.775926634724238 2311 100-101 4.236087710554097 2312 1 -0.3338254303085293 2312 2 -0.24808673000062953 2312 3 -0.14440981297641642 2312 4 -0.10192553968359874 2312 5 -0.07623856326463851 2312 6 -0.0829481531043399 2312 7 -0.08277213729498101 2312 8 -0.03161222900440208 2312 9 -0.007990206006759593 2312 10-11 -0.1602650824113212 2312 12-13 -0.0226165023208722 2312 14-15 0.023228842727888832 2312 16-17 0.05136410365285826 2312 18-19 -0.04978826096908762 2312 20-21 0.001097587908816422 2312 22-23 0.00422382083731776 2312 24-25 0.14555972064782452 2312 26-27 0.07831755674567376 2312 28-29 0.2283310843323889 2312 30-31 0.2734787172664568 2312 32-33 0.2843748015356695 2312 34-35 0.3864918255227465 2312 36-37 0.4958138362998028 2312 38-39 0.45331017790684314 2312 40-41 0.595575954221566 2312 42-43 0.8530963247438663 2312 44-45 1.0957134252233303 2312 46-47 0.8828804801315187 2312 48-49 1.2338360987632342 2312 50-51 1.414554743418158 2312 52-53 1.5951937650394186 2312 54-55 1.702957685087469 2312 56-57 1.8846334704453511 2312 58-59 1.9542544012344614 2312 60-61 2.154612233506157 2312 62-63 2.339148886935835 2312 64-65 2.540304755852887 2312 66-67 2.705126321120151 2312 68-69 3.0832958931855075 2312 70-71 3.35118177792161 2312 72-73 3.4859028006179678 2312 74-75 3.145971058243177 2312 76-77 3.5607111216165563 2312 78-79 4.039587464168921 2312 80-81 4.105715662250866 2312 82-83 4.587757167962643 2312 84-85 4.912376395772416 2312 86-87 5.109677209347925 2312 88-89 5.141372147618444 2312 90-91 5.301757394630073 2312 92-93 5.344235143037686 2312 94-95 5.347612670266795 2312 96-97 5.283477135706887 2312 98-99 4.8892274116759875 2312 100-101 4.318039519971926 2313 1 -0.48970289300858383 2313 2 -0.3102557854247294 2313 3 -0.360976451709206 2313 4 -0.16775578180460116 2313 5 -0.2574100505793666 2313 6 -0.33853980703644737 2313 7 -0.21553996362841588 2313 8 -0.22093152556360707 2313 9 -0.23973613505471292 2313 10-11 -0.20811866677986046 2313 12-13 -0.15867105246218927 2313 14-15 -0.2465549161863052 2313 16-17 -0.16357759464482058 2313 18-19 -0.2588326501619562 2313 20-21 -0.24840342777523006 2313 22-23 -0.21675447084167132 2313 24-25 -0.2291585014840294 2313 26-27 -0.2846322351880062 2313 28-29 -0.12137604640637534 2313 30-31 -0.002366025824017015 2313 32-33 -0.0726529053804299 2313 34-35 0.06000396135533492 2313 36-37 0.14909072676613278 2313 38-39 0.16172772366911659 2313 40-41 0.3801956067323289 2313 42-43 0.4015060192376083 2313 44-45 0.5444310085730919 2313 46-47 0.6941271783881149 2313 48-49 0.7750774669892664 2313 50-51 0.8536728801052007 2313 52-53 0.9419458867818378 2313 54-55 1.137297023177787 2313 56-57 1.5067608766614669 2313 58-59 1.732932070973856 2313 60-61 1.769837301492288 2313 62-63 1.9182691597430228 2313 64-65 2.3094492191937768 2313 66-67 2.2989691345136265 2313 68-69 2.474322699439412 2313 70-71 2.8897440098796103 2313 72-73 3.2881489104145416 2313 74-75 3.3765360876969908 2313 76-77 3.4496876889947146 2313 78-79 3.7573303810820633 2313 80-81 4.068953214216098 2313 82-83 4.2305683534271665 2313 84-85 4.298773187278872 2313 86-87 4.215276899405108 2313 88-89 4.2531768559148055 2313 90-91 4.388936257721202 2313 92-93 4.392999274294052 2313 94-95 4.465319694199554 2313 96-97 4.155477067926155 2313 98-99 4.071364132608322 2313 100-101 3.6472662136687273 2314 1 -0.4317627242866351 2314 2 -0.3603339726861705 2314 3 -0.2706370678746346 2314 4 -0.36821435369756017 2314 5 -0.30296346958444076 2314 6 -0.3349905772432322 2314 7 -0.28752369176481807 2314 8 -0.26719701883151004 2314 9 -0.3259328392617462 2314 10-11 -0.40549415045294523 2314 12-13 -0.33640239631311886 2314 14-15 -0.34661363855533267 2314 16-17 -0.3008598106802225 2314 18-19 -0.3426537527268678 2314 20-21 -0.3621719554691225 2314 22-23 -0.3908246781526046 2314 24-25 -0.3466371556407353 2314 26-27 -0.2531661224515389 2314 28-29 -0.1770153272146473 2314 30-31 -0.20582756529384483 2314 32-33 -0.0797816764575714 2314 34-35 -0.07680621465447501 2314 36-37 0.017476595647472948 2314 38-39 0.09375920305336649 2314 40-41 0.20284274690052229 2314 42-43 0.31904909760050515 2314 44-45 0.543153829815914 2314 46-47 0.6295979469695538 2314 48-49 0.7595535599069265 2314 50-51 0.7268874513794756 2314 52-53 0.9287448229632105 2314 54-55 1.0493604394040332 2314 56-57 1.269217916267607 2314 58-59 1.501081936226985 2314 60-61 1.702616737390528 2314 62-63 1.7850714580421716 2314 64-65 2.00885950504588 2314 66-67 2.107821433337083 2314 68-69 2.506529887162241 2314 70-71 2.91340122722578 2314 72-73 3.160611920565124 2314 74-75 3.476898744202586 2314 76-77 3.6852156725629364 2314 78-79 3.8145193163721096 2314 80-81 4.171315118341344 2314 82-83 4.3463297387507716 2314 84-85 4.487674561225013 2314 86-87 4.6060469030228255 2314 88-89 4.804565646496162 2314 90-91 4.7342047814827914 2314 92-93 4.997196088605531 2314 94-95 5.04614274456447 2314 96-97 5.052878406481584 2314 98-99 4.929533718353467 2314 100-101 4.376332934339002 2315 1 -0.45618114599972515 2315 2 -0.38478829236662193 2315 3 -0.3309412828578857 2315 4 -0.30639742958523897 2315 5 -0.23366884279219846 2315 6 -0.22610954498001234 2315 7 -0.2650612410386799 2315 8 -0.27640094550725536 2315 9 -0.43548282234287683 2315 10-11 -0.3831476657691937 2315 12-13 -0.28750998907658953 2315 14-15 -0.36485292753834386 2315 16-17 -0.31917838734766946 2315 18-19 -0.4275629027753851 2315 20-21 -0.36431053785526757 2315 22-23 -0.33042572375114077 2315 24-25 -0.24186940612489138 2315 26-27 -0.30607441968921023 2315 28-29 -0.2063260175669015 2315 30-31 -0.17550658127748875 2315 32-33 -0.1318371628531807 2315 34-35 -0.04793113833228091 2315 36-37 0.0950588144789819 2315 38-39 0.1102888123211585 2315 40-41 0.14218361027473492 2315 42-43 0.2756250433402627 2315 44-45 0.3331507207397806 2315 46-47 0.5940221248436686 2315 48-49 0.7396554726754871 2315 50-51 0.8666543143716012 2315 52-53 1.1571735782963941 2315 54-55 1.2862633001286596 2315 56-57 1.602600622312579 2315 58-59 1.9731837528514298 2315 60-61 2.1326196029847964 2315 62-63 2.2426608190084103 2315 64-65 2.490245316421106 2315 66-67 2.5715457569257296 2315 68-69 2.8273807688787507 2315 70-71 3.186652028530844 2315 72-73 3.571633970033144 2315 74-75 3.879643190630958 2315 76-77 4.0723092295333245 2315 78-79 4.329831204155685 2315 80-81 4.4991643868344156 2315 82-83 4.67219743944538 2315 84-85 4.819374432991232 2315 86-87 5.007653227807655 2315 88-89 5.1183588317509 2315 90-91 5.213277671212975 2315 92-93 5.408180835403122 2315 94-95 5.377234067744819 2315 96-97 5.2767665371776555 2315 98-99 5.146095229988779 2315 100-101 4.6654832575455725 2316 1 -0.8620831372921778 2316 2 -0.9172458198860731 2316 3 -0.7768992980024478 2316 4 -0.7709630856243663 2316 5 -0.6382252433491047 2316 6 -0.6258378811207024 2316 7 -0.726053505104197 2316 8 -0.6982418227354401 2316 9 -0.8170606850720148 2316 10-11 -0.7592120159185782 2316 12-13 -0.8000658810971686 2316 14-15 -0.7264533137592508 2316 16-17 -0.777279828523838 2316 18-19 -0.8563928207125286 2316 20-21 -0.8655518756149121 2316 22-23 -0.8568817347534647 2316 24-25 -0.94129102940434 2316 26-27 -0.7788809098006411 2316 28-29 -0.7878905607661224 2316 30-31 -0.7778208761182412 2316 32-33 -0.5430767398285425 2316 34-35 -0.5410596095169424 2316 36-37 -0.2663657206103309 2316 38-39 -0.4297691814272966 2316 40-41 -0.4452856972248753 2316 42-43 -0.43035391028715964 2316 44-45 -0.1293667734550823 2316 46-47 -0.0919103301381412 2316 48-49 0.12975419781918873 2316 50-51 0.03067977937320876 2316 52-53 0.3642645384117529 2316 54-55 0.7179777524398858 2316 56-57 0.8770362015499842 2316 58-59 0.9250965373574935 2316 60-61 0.8696680648594217 2316 62-63 1.125881499525768 2316 64-65 1.6609679744113386 2316 66-67 1.7422747034312245 2316 68-69 2.003552008634532 2316 70-71 2.3637588932862457 2316 72-73 2.938213875393263 2316 74-75 3.098685057292613 2316 76-77 3.4576375476115793 2316 78-79 3.723948896673658 2316 80-81 3.797696192780837 2316 82-83 3.8897152124563448 2316 84-85 4.228999225318699 2316 86-87 4.418661266196995 2316 88-89 4.638227736664302 2316 90-91 4.756467194031348 2316 92-93 4.94002921035986 2316 94-95 5.064528327224416 2316 96-97 5.22060509538324 2316 98-99 4.769723576009257 2316 100-101 4.35371852135712 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8569.0 3 3136.0 4 3408.0 5 3858.0 6 4594.0 7 5730.0 8 7480.0 9 9449.0 10 12409.0 11 15554.0 12 19775.0 13 24141.0 14 28836.0 15 35216.0 16 42256.0 17 52149.0 18 52605.0 19 49699.0 20 53090.0 21 57590.0 22 63049.0 23 70158.0 24 77359.0 25 83987.0 26 90593.0 27 96909.0 28 104279.0 29 112573.0 30 120402.0 31 132082.0 32 147007.0 33 168627.0 34 199087.0 35 245998.0 36 292580.0 37 302269.0 38 153471.0 39 9867.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.49259559856725 18.595268224073063 15.131447076072876 22.780689101286814 2 15.52787936503216 19.68347581832842 40.21760361618334 24.57104120045608 3 19.226758737707385 23.941816780656968 29.581877823351366 27.24954665828428 4 13.052853506685057 16.73689308673929 36.517524384345755 33.6927290222299 5 14.03936249005729 36.96843043245598 33.727875459274685 15.26433161821204 6 32.2882612694808 35.885949484033205 16.933182747944468 14.892606498541527 7 28.717322014754753 32.93999042215193 20.041258422439878 18.301429140653436 8 26.87715404055595 34.026693036167266 19.386212923803996 19.709939999472788 9 26.47751452572723 16.897023476387073 20.135672590513735 36.48978940737196 10-11 25.182638394397543 26.450402485443508 26.452666772333387 21.91429234782556 12-13 26.45124823840728 23.751119477385714 27.02923652487183 22.768395759335174 14-15 23.098206680597603 25.05870407822051 25.724002883964044 26.119086357217842 16-17 22.255574454653264 28.225789281406588 26.599000537007743 22.91963572693241 18-19 22.292250809280766 26.344754451424397 29.36464996727055 21.998344772024282 20-21 22.74837571231312 25.49897195056643 29.8698085700472 21.88284376707325 22-23 22.59121993354763 25.34653977274263 29.801981134881007 22.26025915882873 24-25 22.455308667013128 25.606344458105706 29.730546436312473 22.207800438568697 26-27 21.856795769905375 25.952676247323787 29.63781952540377 22.552708457367068 28-29 22.100037259870277 26.401093632110744 29.386310503988327 22.112558604030657 30-31 22.156195495067234 25.85434752594395 29.349659457447096 22.639797521541727 32-33 21.904137460203728 25.832054670442943 29.40092672078734 22.862881148565982 34-35 22.755833274611504 25.98571986458999 29.05569719204878 22.20274966874973 36-37 22.864737021228848 25.48702182029443 29.064761963343205 22.583479195133517 38-39 22.291193871082417 25.96286411736639 28.976703652290976 22.769238359260218 40-41 22.75257359922743 25.78836644619956 29.077971288141068 22.38108866643194 42-43 22.48581551476803 25.795920132685314 28.80097152087518 22.917292831671475 44-45 22.4174760758841 26.107780667841162 28.957793135885463 22.51695012038927 46-47 22.999948299649823 26.401341370654002 28.13992615297694 22.45878417671923 48-49 22.720851053477045 26.472086930018467 27.964515560048902 22.842546456455583 50-51 22.833142343363306 26.69742441451564 27.9718115360645 22.497621706056552 52-53 23.141249370719628 26.404570687191057 27.94687353238302 22.507306409706292 54-55 22.667630948374775 26.6518645707899 28.015052795606067 22.66545168522926 56-57 22.75871867507494 26.727955983597916 27.794476595675665 22.718848745651474 58-59 22.76993524625294 26.282240248885756 27.812740360128206 23.1350841447331 60-61 22.41681098220556 26.605298141459134 28.09932085115843 22.878570025176874 62-63 22.54484022738983 26.55178355731626 28.290673807090304 22.612702408203603 64-65 22.251863705191376 26.625588312857506 28.038806221794392 23.083741760156723 66-67 22.299635322019594 26.64706918813157 27.929616659872703 23.123678829976136 68-69 22.796544526764546 26.304895591627737 28.09137221346087 22.807187668146852 70-71 22.776362114531192 26.415335659744315 27.57734719538169 23.230955030342802 72-73 22.75366366322226 26.492755121711557 27.569869321362134 23.183711893704054 74-75 22.669915821349203 26.95152859955554 27.370783084798006 23.007772494297253 76-77 22.94307029255579 26.516261274449267 27.514124685116197 23.02654374787874 78-79 22.987002982418026 26.7058015413158 27.36342353343048 22.943771942835692 80-81 23.220613184006897 26.753623207993236 26.968736664165206 23.057026943834657 82-83 23.225103314005477 26.71122981552404 27.05001494317286 23.01365192729762 84-85 23.15964276996031 26.818657523902196 27.048121991372486 22.973577714765007 86-87 23.434789747775714 26.710978956195397 26.762096825962068 23.09213447006682 88-89 23.32278535552284 26.79854905603822 26.60055481642362 23.278110772015328 90-91 23.351617669545472 26.761797029506408 26.607874063642328 23.278711237305792 92-93 23.4667366341023 26.309084863786513 26.779540404504587 23.4446380976066 94-95 23.359853416920654 26.0913893592769 26.922542724391977 23.62621449941047 96-97 23.626758796453288 25.800614908203904 26.868788551089807 23.703837744252997 98-99 23.389599238479107 26.04677865781605 26.418449242584753 24.14517286112009 100-101 24.27025225340706 24.86182603888572 26.69918501756779 24.16873669013943 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1295.0 1 875.5 2 630.5 3 794.0 4 642.5 5 584.0 6 1107.0 7 2681.0 8 4667.0 9 5435.5 10 4466.5 11 3329.5 12 3186.5 13 3407.5 14 3660.5 15 3973.0 16 4220.5 17 4460.0 18 4859.0 19 5417.0 20 6173.5 21 7257.0 22 8688.5 23 10480.5 24 12849.5 25 15764.0 26 19492.0 27 23695.0 28 26942.5 29 30139.5 30 34173.5 31 39359.5 32 45984.0 33 49292.5 34 49884.5 35 52932.5 36 58986.0 37 64855.5 38 68758.5 39 71956.5 40 75721.5 41 80320.5 42 83092.0 43 85806.0 44 89689.0 45 94846.5 46 98358.0 47 101237.5 48 104087.5 49 103328.0 50 101804.5 51 100431.0 52 100599.0 53 102842.5 54 105332.5 55 103007.0 56 97091.5 57 90264.5 58 84506.5 59 77157.0 60 64906.0 61 54792.0 62 46997.5 63 37101.0 64 27673.5 65 21440.5 66 16558.5 67 12753.0 68 9979.5 69 7948.5 70 6219.5 71 4586.5 72 3426.5 73 2648.5 74 1958.0 75 1420.5 76 1046.0 77 730.0 78 470.5 79 323.5 80 212.5 81 127.0 82 88.0 83 57.0 84 32.5 85 23.5 86 17.5 87 14.0 88 11.0 89 9.5 90 10.5 91 5.5 92 2.5 93 3.5 94 4.0 95 4.0 96 3.5 97 1.5 98 0.0 99 0.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.384885539459721 2 0.023616133434194605 3 0.042434711864590025 4 0.03642087531053188 5 0.05554352412849204 6 0.054327242578233084 7 0.031420606715022864 8 0.029292114002069703 9 0.032197675483243864 10-11 0.028785330022795142 12-13 0.02942725639654292 14-15 0.024308738205869842 16-17 0.028785330022795142 18-19 0.024731058188598644 20-21 0.03657291050431425 22-23 0.02462970139274373 24-25 0.04572880772987468 26-27 0.03839733282970268 28-29 0.030187432365454764 30-31 0.03738376487115355 32-33 0.034495096189288546 34-35 0.032957851452155705 36-37 0.033447742632121115 38-39 0.01665630011882395 40-41 0.012399314692917628 42-43 0.012078351506043737 44-45 0.009291039620033643 46-47 0.016217087336785996 48-49 0.013615596243176577 50-51 0.008834934038686537 52-53 0.003750201446631762 54-55 0.00422319982728802 56-57 0.007331474900172003 58-59 0.003023811076338222 60-61 0.0060983005506039 62-63 0.00560840937063849 64-65 0.007179439706389634 66-67 0.0035981662528493928 68-69 0.0063685853395503336 70-71 0.016554943322969036 72-73 0.013700060239722336 74-75 0.010203250782727857 76-77 0.007348367699481154 78-79 0.005473266976165274 80-81 0.008108543668392998 82-83 0.009983644391708879 84-85 0.004172521429360564 86-87 5.067839792745624E-4 88-89 0.02243363748255396 90-91 0.019207112814505913 92-93 0.012466885890154234 94-95 0.02364991903281291 96-97 0.018159759257338485 98-99 0.01689279930915208 100-101 0.10647531404558556 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2959841.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.29914423708387 #Duplication Level Percentage of deduplicated Percentage of total 1 73.20296672894703 35.35640648623865 2 14.064711298887191 13.586270393557914 3 5.136788572335806 7.443074765119538 4 2.4328730555195257 4.700227464762102 5 1.2809995000082974 3.0935589809266544 6 0.842208224019963 2.440676189375907 7 0.5549437795158186 1.8762316755214896 8 0.3786318691050027 1.463007620692735 9 0.29358503995143825 1.2761915571418097 >10 1.6293377661649462 14.355334433954633 >50 0.1194517107931522 3.9635610855934074 >100 0.05666384743166027 5.074186610933617 >500 0.0029610464685719973 1.0024400791473633 >1k 0.0036660575323523023 3.1682132491234576 >5k 7.050110639139044E-5 0.2634559221477203 >10k+ 1.4100221278278089E-4 0.9371634857631586 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15405 0.5204671467149755 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12117 0.4093800984579915 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7737 0.26139917650981925 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 3801 0.1284190603481741 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 3764 0.12716899319929686 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 3538 0.1195334479115601 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 3486 0.1177765967834083 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 3389 0.11449939371743278 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3164 0.10689763402831436 No Hit GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 3116 0.10527592529463575 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.0135679585491247E-4 2 0.0 0.0 0.0 0.0 1.0135679585491247E-4 3 0.0 0.0 0.0 0.0 1.0135679585491247E-4 4 0.0 0.0 0.0 0.0 1.689279930915208E-4 5 0.0 0.0 0.0 0.0 2.0271359170982495E-4 6 0.0 0.0 0.0 0.0 2.0271359170982495E-4 7 0.0 0.0 0.0 0.0 2.0271359170982495E-4 8 0.0 0.0 0.0 0.0 2.0271359170982495E-4 9 0.0 0.0 0.0 0.0 2.0271359170982495E-4 10-11 0.0 0.0 0.0 0.0 2.0271359170982495E-4 12-13 0.0 0.0 0.0 0.0 4.22319982728802E-4 14-15 0.0 0.0 0.0 0.0 4.898911799654103E-4 16-17 0.0 0.0 0.0 3.378559861830416E-5 5.067839792745624E-4 18-19 5.0678397927456236E-5 0.0 0.0 1.0135679585491247E-4 5.067839792745624E-4 20-21 1.0135679585491247E-4 0.0 0.0 1.8582079240067286E-4 5.743551765111707E-4 22-23 1.3514239447321664E-4 0.0 0.0 2.8717758825558536E-4 6.25033574438627E-4 24-25 1.689279930915208E-4 0.0 0.0 4.5610558134710614E-4 6.757119723660832E-4 26-27 1.8582079240067286E-4 0.0 0.0 0.0011656031523314936 7.263903702935395E-4 28-29 2.533919896372812E-4 0.0 0.0 0.0030407038756473746 7.432831696026914E-4 30-31 2.702847889464333E-4 0.0 0.0 0.010000537191018032 7.770687682209956E-4 32-33 2.702847889464333E-4 0.0 0.0 0.023852632624522738 8.953183633850602E-4 34-35 3.209631868738895E-4 0.0 0.0 0.044174670193432686 9.797823599308206E-4 36-37 3.716415848013457E-4 0.0 0.0 0.07679466565940535 9.797823599308206E-4 38-39 4.054271834196499E-4 0.0 0.0 0.1263074604345301 9.797823599308206E-4 40-41 4.054271834196499E-4 0.0 0.0 0.17772914153158903 0.0010135679585491248 42-43 4.22319982728802E-4 0.0 0.0 0.23193813451465806 0.001047353557167429 44-45 5.405695778928666E-4 0.0 0.0 0.29966136694504875 0.0010811391557857331 46-47 6.081407751294749E-4 0.0 0.0 0.3794122724835557 0.0011656031523314936 48-49 6.081407751294749E-4 0.0 0.0 0.45845368045107826 0.001233174349568102 50-51 6.081407751294749E-4 0.0 0.0 0.5466172000455429 0.0012838527474955581 52-53 6.081407751294749E-4 0.0 0.0 0.6306926622071929 0.0014189951419687746 54-55 6.081407751294749E-4 0.0 0.0 0.7210353529125382 0.001520351937823687 56-57 6.419263737477791E-4 0.0 0.0 0.8283553069235814 0.0015372447371328393 58-59 6.419263737477791E-4 0.0 0.0 0.9401180671529314 0.0015541375364419912 60-61 6.419263737477791E-4 0.0 0.0 1.0614252589919526 0.0015541375364419912 62-63 6.419263737477791E-4 0.0 0.0 1.178543036602304 0.0016217087336785996 64-65 6.588191730569311E-4 3.378559861830416E-5 0.0 1.2983467693028103 0.0016217087336785996 66-67 6.757119723660832E-4 3.378559861830416E-5 0.0 1.4398915347141958 0.0016217087336785996 68-69 6.757119723660832E-4 3.378559861830416E-5 0.0 1.5953390739570132 0.0016217087336785996 70-71 7.094975709843873E-4 3.378559861830416E-5 0.0 1.7566822001587248 0.0016386015329877515 72-73 7.601759689118435E-4 3.378559861830416E-5 0.0 1.92233299018427 0.001723065529533512 74-75 7.770687682209956E-4 3.378559861830416E-5 0.0 2.0908724488916803 0.0017906367267701204 76-77 7.770687682209956E-4 3.378559861830416E-5 0.0 2.2713044383127334 0.0017906367267701204 78-79 7.770687682209956E-4 3.378559861830416E-5 0.0 2.468325156655374 0.0017906367267701204 80-81 8.44639965457604E-4 3.378559861830416E-5 0.0 2.6801777527914505 0.0018413151246975765 82-83 8.44639965457604E-4 3.378559861830416E-5 0.0 2.8967603327340896 0.0019595647198616412 84-85 8.44639965457604E-4 3.378559861830416E-5 0.0 3.1152517989986626 0.001976457519170793 86-87 8.44639965457604E-4 3.378559861830416E-5 0.0 3.34560201037826 0.002010243117789097 88-89 8.44639965457604E-4 3.378559861830416E-5 3.378559861830416E-5 3.577624608889464 0.0020271359170982496 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7435 0.0 52.42064 1 GTATCAA 13040 0.0 41.595284 1 TATCAAC 16560 0.0 32.51657 2 ATCAACG 16610 0.0 32.390076 3 TCAACGC 16825 0.0 31.919674 4 CAACGCA 17115 0.0 31.295517 5 AACGCAG 17585 0.0 30.4794 6 ACGCAGA 19700 0.0 27.00868 7 CGCAGAG 19840 0.0 26.770205 8 TAAGGTG 1935 0.0 24.807074 5 GCAGAGT 21435 0.0 24.222496 9 GAGTACT 12985 0.0 23.90684 12-13 TGGTATC 3495 0.0 23.797207 2 AGGTAAG 1810 0.0 23.631924 2 GTAAGGT 2000 0.0 23.287947 4 GTACTTT 13535 0.0 22.759317 14-15 GTGGTAT 3890 0.0 22.564695 1 CAGAGTA 20230 0.0 21.802597 10-11 AGTACTT 13255 0.0 21.68174 12-13 TACATGG 10830 0.0 21.59095 2 >>END_MODULE