FastQCFastQC Report
Thu 26 May 2016
SRR938291_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938291_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2959841
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT146630.4953982325401939No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT122280.4131302999046233No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79190.26754815545835064No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC41810.1412575878231297No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA36960.12487157249325216No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA36430.12308093576648205No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA35990.12159436942727667No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA34090.11517510568979888No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32440.10960048191777869No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA71600.051.0885851
GTATCAA124500.042.142151
TATCAAC161150.032.4397852
ATCAACG160900.032.3341643
TCAACGC162600.032.024274
CAACGCA165200.031.4898725
AACGCAG170250.030.5744386
ACGCAGA192900.026.8613347
CGCAGAG193800.026.5160338
TAAGGTG21200.025.794415
GTAAGGT21850.024.3754274
GCAGAGT210300.024.1871819
TGGTATC35200.023.3783552
GAGTACT130250.023.30012112-13
AGGTAAG22150.023.1931932
AAGGTAA22100.022.5999551
GGTAAGG23600.022.165683
AGAGTAC183650.022.05937410-11
GTACTTT136650.021.87868314-15
AGTACTT134500.021.87530312-13
Produced by FastQC (version 0.11.5)