##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938291_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2959841 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.48573690275931 33.0 31.0 34.0 31.0 34.0 2 32.71138584809116 34.0 31.0 34.0 31.0 34.0 3 32.77411658261373 34.0 31.0 34.0 31.0 34.0 4 36.23560184482883 37.0 35.0 37.0 35.0 37.0 5 36.15968188831765 37.0 35.0 37.0 35.0 37.0 6 36.21524399452538 37.0 37.0 37.0 35.0 37.0 7 36.188896295442895 37.0 37.0 37.0 35.0 37.0 8 36.18420753006665 37.0 37.0 37.0 35.0 37.0 9 37.87463481991094 39.0 38.0 39.0 35.0 39.0 10-11 37.847417141664025 39.0 38.0 39.0 35.0 39.0 12-13 37.76726351178999 39.0 38.0 39.0 35.0 39.0 14-15 39.00017534725683 40.0 38.0 41.0 36.0 41.0 16-17 38.90753523584544 40.0 38.0 41.0 35.5 41.0 18-19 39.16964205171832 40.0 39.0 41.0 36.0 41.0 20-21 39.14198634318532 40.0 39.0 41.0 36.0 41.0 22-23 39.03571171559553 40.0 39.0 41.0 35.0 41.0 24-25 38.92206963144304 40.0 38.0 41.0 35.0 41.0 26-27 38.751322283865925 40.0 38.0 41.0 35.0 41.0 28-29 38.62497985533682 40.0 38.0 41.0 34.5 41.0 30-31 38.36360787623389 40.0 38.0 41.0 34.0 41.0 32-33 38.117675071059566 40.0 37.5 41.0 34.0 41.0 34-35 38.018007048351585 40.0 37.5 41.0 33.0 41.0 36-37 37.947362713064656 40.0 37.0 41.0 33.0 41.0 38-39 37.74405601517108 40.0 37.0 41.0 33.0 41.0 40-41 37.506661506479574 40.0 37.0 41.0 33.0 41.0 42-43 37.22743823063469 40.0 36.5 41.0 32.0 41.0 44-45 36.93139699058159 40.0 36.0 41.0 31.0 41.0 46-47 36.533859926935264 39.0 35.5 41.0 30.5 41.0 48-49 36.080522568610945 39.0 35.0 40.5 29.5 41.0 50-51 35.666209772754684 39.0 35.0 40.0 28.0 41.0 52-53 35.113839223120436 38.0 34.5 40.0 26.5 41.0 54-55 34.71575635312843 38.0 34.0 40.0 26.0 41.0 56-57 34.31350552276288 38.0 34.0 40.0 25.0 41.0 58-59 33.78341184543359 37.0 33.0 40.0 23.0 41.0 60-61 33.28439145886553 36.5 32.5 39.0 22.0 41.0 62-63 32.710111624239275 36.0 32.0 39.0 19.5 40.0 64-65 32.11977079174186 35.0 31.0 39.0 17.5 40.0 66-67 31.543669406566096 35.0 31.0 38.0 12.0 40.0 68-69 30.900661724734537 35.0 30.0 37.5 7.5 40.0 70-71 30.256005305690408 34.0 29.0 37.0 6.0 39.0 72-73 29.579623702759708 34.0 28.5 36.0 2.0 39.0 74-75 28.91506182257763 34.0 27.5 36.0 2.0 38.5 76-77 27.63851014294349 31.5 26.0 34.5 2.0 36.5 78-79 28.292453716263815 33.0 27.0 35.0 2.0 37.0 80-81 28.022761019933164 33.0 26.5 35.0 2.0 37.0 82-83 27.465393242407277 33.0 26.0 35.0 2.0 36.0 84-85 26.80332592189918 33.0 25.0 35.0 2.0 36.0 86-87 26.092265260194722 32.0 23.5 35.0 2.0 35.0 88-89 25.268490266875823 31.5 20.0 34.0 2.0 35.0 90-91 24.602052103474477 31.0 17.5 34.0 2.0 35.0 92-93 23.654074492514972 31.0 4.5 34.0 2.0 35.0 94-95 22.732482420508397 30.0 2.0 34.0 2.0 35.0 96-97 21.679146785249614 29.5 2.0 34.0 2.0 35.0 98-99 20.24838006501025 29.0 2.0 34.0 2.0 35.0 100-101 16.420998121182862 13.5 2.0 31.0 2.0 33.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2045144265998715 1101 2 0.20380274906928264 1101 3 0.15894635168628213 1101 4 0.08594033901074027 1101 5 0.016227772424741715 1101 6 0.0836332565438127 1101 7 0.14212843648534346 1101 8 0.14697418336002244 1101 9 0.04743126599630898 1101 10-11 0.21505613480967867 1101 12-13 0.2497450249058204 1101 14-15 0.38530994094141136 1101 16-17 0.40564165165069 1101 18-19 0.31130730850697574 1101 20-21 0.23253364024663625 1101 22-23 0.3384197363121402 1101 24-25 0.3912729666014769 1101 26-27 0.4110164935893721 1101 28-29 0.37150670381551976 1101 30-31 0.4747957558716962 1101 32-33 0.5532094801010388 1101 34-35 0.5448283258147697 1101 36-37 0.5719687899097536 1101 38-39 0.7884869086076947 1101 40-41 0.9189811269909853 1101 42-43 1.0267423061535794 1101 44-45 1.0285378017906055 1101 46-47 1.1324968994551696 1101 48-49 1.1810453215036532 1101 50-51 1.2141825300157194 1101 52-53 1.2919378412916487 1101 54-55 1.4369728983565295 1101 56-57 1.3429048822793916 1101 58-59 1.648781269896304 1101 60-61 1.5513246934958502 1101 62-63 1.6065225813781652 1101 64-65 1.7993006749642575 1101 66-67 2.1434108033737935 1101 68-69 2.2919921950773308 1101 70-71 2.348763408503917 1101 72-73 2.5144901707996077 1101 74-75 2.6822595511889737 1101 76-77 2.6494819510588563 1101 78-79 2.903927221889983 1101 80-81 2.9530961135710143 1101 82-83 3.000248896935222 1101 84-85 3.0991835894113144 1101 86-87 3.2598243022036613 1101 88-89 3.612297958067053 1101 90-91 3.7727193191769643 1101 92-93 3.8183342767788133 1101 94-95 4.270197702644317 1101 96-97 4.367564895000143 1101 98-99 4.493677558719394 1101 100-101 4.269902475401782 1102 1 0.2223700409160756 1102 2 0.17281458785010528 1102 3 0.20138020489500263 1102 4 0.1281818329567983 1102 5 0.03591932797945674 1102 6 0.07163338667848507 1102 7 0.12117217405424441 1102 8 0.114730001438069 1102 9 0.22259301044528712 1102 10-11 0.2055148468996677 1102 12-13 0.32606766856012825 1102 14-15 0.41314707423880037 1102 16-17 0.43780292009735433 1102 18-19 0.39610900000504756 1102 20-21 0.2971635097550376 1102 22-23 0.38049382400333087 1102 24-25 0.4107855869800048 1102 26-27 0.4216742706680421 1102 28-29 0.37871385894836607 1102 30-31 0.40780190473502387 1102 32-33 0.36867914466628093 1102 34-35 0.42402118129484023 1102 36-37 0.47388767620963534 1102 38-39 0.427968931544882 1102 40-41 0.5869346107941169 1102 42-43 0.6299346012964833 1102 44-45 0.6622623130038221 1102 46-47 0.6124818234164096 1102 48-49 0.8049201856440007 1102 50-51 0.8184054001738517 1102 52-53 0.7826600603026179 1102 54-55 0.9432782707391141 1102 56-57 0.9721381679493319 1102 58-59 1.1100677048411285 1102 60-61 1.1092627348616162 1102 62-63 1.2711870060644586 1102 64-65 1.174391366513177 1102 66-67 1.3519512229823043 1102 68-69 1.5559103668999548 1102 70-71 1.75842980889988 1102 72-73 2.023999398832512 1102 74-75 2.140638407968499 1102 76-77 2.1762701894112517 1102 78-79 2.150741133412936 1102 80-81 2.3567050923581156 1102 82-83 2.26911597668472 1102 84-85 2.5009527576643897 1102 86-87 2.7299976403254433 1102 88-89 3.086355279232162 1102 90-91 3.2933575097238155 1102 92-93 3.2734577925881254 1102 94-95 3.617212233191381 1102 96-97 3.7670500733873027 1102 98-99 3.9368374496968634 1102 100-101 3.8696426482597914 1103 1 0.24882057732354923 1103 2 0.2007559413973965 1103 3 0.20541974595267476 1103 4 0.12810442514376774 1103 5 0.055274148192644645 1103 6 0.07154164408526498 1103 7 0.14365771026103147 1103 8 0.1510055961880994 1103 9 0.22368532069580027 1103 10-11 0.24678037863399993 1103 12-13 0.2778698371256709 1103 14-15 0.4245202888419328 1103 16-17 0.4206040508248208 1103 18-19 0.36578807294613824 1103 20-21 0.3308330414664411 1103 22-23 0.3105802340583139 1103 24-25 0.35920618089861733 1103 26-27 0.41375860504685846 1103 28-29 0.34528228458812293 1103 30-31 0.3828178163410385 1103 32-33 0.4077127511249685 1103 34-35 0.5054656684250745 1103 36-37 0.44468486200559454 1103 38-39 0.570420809687775 1103 40-41 0.62014542953942 1103 42-43 0.7609043205068104 1103 44-45 0.8110616318150647 1103 46-47 0.9002539692183191 1103 48-49 1.0238681847953828 1103 50-51 1.1592606704991297 1103 52-53 1.1700116236537426 1103 54-55 1.3096179132870276 1103 56-57 1.2779520680989762 1103 58-59 1.4815979712960257 1103 60-61 1.5472074714170319 1103 62-63 1.654438822281115 1103 64-65 1.7304933957446664 1103 66-67 1.9195784154869422 1103 68-69 2.010732873651637 1103 70-71 1.9712539899538974 1103 72-73 2.1768869969540816 1103 74-75 2.205835855230845 1103 76-77 2.184471117158221 1103 78-79 2.094971671081872 1103 80-81 2.372122148452977 1103 82-83 2.6166799241391487 1103 84-85 2.9573062189404133 1103 86-87 3.0573337794530886 1103 88-89 3.2871984510029684 1103 90-91 3.5881063930444412 1103 92-93 3.7127055606628474 1103 94-95 3.962129980222649 1103 96-97 4.150640190416446 1103 98-99 4.670442761593009 1103 100-101 4.443088328043739 1104 1 0.16254020072931752 1104 2 0.14488322233904682 1104 3 0.1354047000438996 1104 4 0.10119426021795164 1104 5 0.038531348495240536 1104 6 0.13718928222566973 1104 7 0.09828560041434287 1104 8 0.13800365992072727 1104 9 0.1884757257798313 1104 10-11 0.21756938940009718 1104 12-13 0.23688931520796785 1104 14-15 0.3224659595161299 1104 16-17 0.29489510492147275 1104 18-19 0.3223242699332758 1104 20-21 0.3022927485455469 1104 22-23 0.220473329217306 1104 24-25 0.2781258940634217 1104 26-27 0.31078015135314985 1104 28-29 0.36295962252790304 1104 30-31 0.30210593814742026 1104 32-33 0.3654078839510859 1104 34-35 0.37811268558471767 1104 36-37 0.41314087957736234 1104 38-39 0.42790658084017963 1104 40-41 0.5044290954914317 1104 42-43 0.4894005613121095 1104 44-45 0.46842461910772215 1104 46-47 0.41155102584369274 1104 48-49 0.3895481772745697 1104 50-51 0.5713822634681449 1104 52-53 0.627638852312046 1104 54-55 0.7015604132930946 1104 56-57 0.6456121946147206 1104 58-59 0.7807943625604068 1104 60-61 0.7908257992084202 1104 62-63 0.8559705462951754 1104 64-65 0.9767668335600632 1104 66-67 1.0757995143104715 1104 68-69 1.1703444018962763 1104 70-71 1.214848708207949 1104 72-73 1.3074018746003269 1104 74-75 1.3488729889673898 1104 76-77 1.3414157662741353 1104 78-79 1.5384377423221913 1104 80-81 1.666639215010047 1104 82-83 1.8188988212128372 1104 84-85 1.986256076705434 1104 86-87 2.1367880039839235 1104 88-89 2.2691086951027586 1104 90-91 2.505962036487073 1104 92-93 2.806403540328404 1104 94-95 3.1428385915981067 1104 96-97 3.6079488686404204 1104 98-99 3.6373408350119654 1104 100-101 3.2961338656343315 1105 1 0.22348125360507254 1105 2 0.26239878200972555 1105 3 0.2178423526671125 1105 4 0.12446070149275101 1105 5 0.06517945595091845 1105 6 0.04829264062934868 1105 7 0.1459140928926459 1105 8 0.12016338411232397 1105 9 0.19452384461062167 1105 10-11 0.15824127767283613 1105 12-13 0.2523706342114522 1105 14-15 0.3686721687780192 1105 16-17 0.3761295043673769 1105 18-19 0.3135899956004877 1105 20-21 0.24570385866859823 1105 22-23 0.2822723756255314 1105 24-25 0.2854286248393123 1105 26-27 0.2551574236666667 1105 28-29 0.3585493361154306 1105 30-31 0.3897588700434653 1105 32-33 0.4465303055209233 1105 34-35 0.4605951950345357 1105 36-37 0.5070503864793423 1105 38-39 0.4454192638944505 1105 40-41 0.5113186574589363 1105 42-43 0.4992733977911783 1105 44-45 0.7374751426059305 1105 46-47 0.7513397140685214 1105 48-49 0.8805473233529568 1105 50-51 0.898815490174016 1105 52-53 0.9724656911693828 1105 54-55 1.0687511057708505 1105 56-57 1.089728798195047 1105 58-59 1.2730370213105502 1105 60-61 1.238235931544601 1105 62-63 1.3416470325568426 1105 64-65 1.2996244669244064 1105 66-67 1.4147227219190306 1105 68-69 1.6113019158481912 1105 70-71 1.7100353014416392 1105 72-73 1.8396930759239396 1105 74-75 1.8863003297745848 1105 76-77 1.904315611028796 1105 78-79 2.035839198835827 1105 80-81 2.2393945577339345 1105 82-83 2.198411283332458 1105 84-85 2.4315564276394994 1105 86-87 2.484772881650887 1105 88-89 2.410763598072119 1105 90-91 2.7111024843276468 1105 92-93 2.7353429055891993 1105 94-95 2.949258923575403 1105 96-97 3.2286193146643924 1105 98-99 3.150755062126219 1105 100-101 2.76339790526281 1106 1 0.1184866776193374 1106 2 0.03481281932599245 1106 3 0.06806396562166128 1106 4 0.08245940535488216 1106 5 -0.08876761787531962 1106 6 -0.03768856474371063 1106 7 -1.676265782677433E-4 1106 8 0.03750012592911389 1106 9 0.08511647294892555 1106 10-11 0.05413906787203615 1106 12-13 0.041116171909642674 1106 14-15 -0.07544466244240766 1106 16-17 0.0014808033117432728 1106 18-19 -0.09622934749293677 1106 20-21 -0.09898129171265424 1106 22-23 0.051850342236114955 1106 24-25 0.04875877245110871 1106 26-27 -0.1274187263166553 1106 28-29 0.03411392269554625 1106 30-31 0.1195139989358438 1106 32-33 0.20351845631944343 1106 34-35 0.20912291326053634 1106 36-37 0.26384685403275654 1106 38-39 0.1535224737380645 1106 40-41 0.21223490713762772 1106 42-43 0.2185023979279208 1106 44-45 0.3620796742665675 1106 46-47 0.556497870639511 1106 48-49 0.5251978539696012 1106 50-51 0.6428779870739945 1106 52-53 0.5593126417984777 1106 54-55 0.7241497108592938 1106 56-57 0.800707821782062 1106 58-59 0.7964659501484235 1106 60-61 0.7083037106898331 1106 62-63 0.7373250117370134 1106 64-65 0.8653566259734049 1106 66-67 1.0819557739620613 1106 68-69 1.0776655559789425 1106 70-71 0.835601890869949 1106 72-73 1.194943972528126 1106 74-75 1.5481960739293754 1106 76-77 1.5460989922609656 1106 78-79 1.6932337457394517 1106 80-81 1.5145462185471352 1106 82-83 1.9339368752745543 1106 84-85 2.0972457169473415 1106 86-87 2.3831477995121375 1106 88-89 2.4520540400661837 1106 90-91 2.462859057530995 1106 92-93 2.385136172729343 1106 94-95 2.699276409256292 1106 96-97 3.1545841255635025 1106 98-99 3.3963422350576558 1106 100-101 3.1789335905552107 1107 1 0.18375735096650203 1107 2 0.21185304646812853 1107 3 0.1977602048121625 1107 4 0.13373021720335743 1107 5 0.013440978813250126 1107 6 0.07745829966788165 1107 7 0.14441039051978066 1107 8 0.14910971695722708 1107 9 0.13004617105975314 1107 10-11 0.1879638312812446 1107 12-13 0.16407076656560804 1107 14-15 0.3834134005482355 1107 16-17 0.31950163216093586 1107 18-19 0.22744938438413698 1107 20-21 0.2378868194851762 1107 22-23 0.29189556104009995 1107 24-25 0.32984273855447555 1107 26-27 0.29329121140143144 1107 28-29 0.32529176398427495 1107 30-31 0.3827654230973394 1107 32-33 0.45331971942638205 1107 34-35 0.48091693561914184 1107 36-37 0.5064134204419517 1107 38-39 0.536980551826602 1107 40-41 0.6545995236730704 1107 42-43 0.6117083474947407 1107 44-45 0.6555349885678226 1107 46-47 0.7193063401813973 1107 48-49 0.8455859945147353 1107 50-51 0.8549338392843779 1107 52-53 0.9812297868249402 1107 54-55 1.0707320664170936 1107 56-57 1.0759185215692568 1107 58-59 1.2351464320081789 1107 60-61 1.153798082358712 1107 62-63 1.3166822658675628 1107 64-65 1.4495712206418503 1107 66-67 1.5933969174587723 1107 68-69 1.7950124699265793 1107 70-71 1.8162223143220864 1107 72-73 2.0454803531396912 1107 74-75 1.9295662136721994 1107 76-77 1.8914415115996768 1107 78-79 1.782065986347412 1107 80-81 2.0235478050733278 1107 82-83 2.225983843437348 1107 84-85 2.274458322063282 1107 86-87 2.5175673074831337 1107 88-89 2.5961177930896646 1107 90-91 2.800151032628211 1107 92-93 3.2073844731249466 1107 94-95 3.3555370737007024 1107 96-97 3.543910291766313 1107 98-99 3.730096250503667 1107 100-101 3.7907387678836706 1108 1 0.1070815508621692 1108 2 0.14115985906720852 1108 3 0.13368487674279805 1108 4 0.0681854777089157 1108 5 -0.01807393941631119 1108 6 0.0537803267845689 1108 7 0.049295851133734914 1108 8 0.08125912210200426 1108 9 0.13655450511059541 1108 10-11 0.13779631458022124 1108 12-13 0.23652788077072984 1108 14-15 0.2534008146373665 1108 16-17 0.1907492833818054 1108 18-19 0.2236038441522723 1108 20-21 0.24858997178736075 1108 22-23 0.24403238027399965 1108 24-25 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0.3143941887953048 2316 60-61 0.446255795368522 2316 62-63 0.4078298961826121 2316 64-65 0.5555250704076968 2316 66-67 0.7557766892679574 2316 68-69 1.0607161677616794 2316 70-71 1.1609891815341236 2316 72-73 1.2014143480440929 2316 74-75 1.612869531221481 2316 76-77 1.6582196907687887 2316 78-79 1.6449091348743359 2316 80-81 1.5982206346374603 2316 82-83 1.9666299331890507 2316 84-85 2.2194179891586074 2316 86-87 2.3558240506481845 2316 88-89 2.5536276166567546 2316 90-91 2.9853109971116787 2316 92-93 2.969585599489786 2316 94-95 3.3070187487746843 2316 96-97 3.582160561570209 2316 98-99 3.9897390465705698 2316 100-101 4.404576446903505 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6.0 3 6.0 4 19.0 5 50.0 6 105.0 7 268.0 8 763.0 9 1473.0 10 2515.0 11 4280.0 12 6563.0 13 10008.0 14 13547.0 15 18984.0 16 25316.0 17 34908.0 18 35844.0 19 29511.0 20 28102.0 21 29612.0 22 31916.0 23 35028.0 24 39516.0 25 44771.0 26 49986.0 27 58043.0 28 68762.0 29 83278.0 30 99683.0 31 118755.0 32 143785.0 33 180193.0 34 235186.0 35 319264.0 36 434488.0 37 513235.0 38 251878.0 39 10194.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.7807439656387 18.972877259285212 15.519853938100054 22.72652483697604 2 15.813831527101584 19.969966722804475 39.59185425074948 24.62434749934446 3 19.28082249634262 23.791578399672947 29.802552225341834 27.125046878642596 4 13.30497768927291 17.012372849803043 36.250108538215756 33.432540922708284 5 14.03294551552754 36.89537848850837 33.87510794170184 15.196568054262247 6 32.43434360156508 35.96909428580792 16.762353112886807 14.83420899974019 7 28.77286313690499 32.855244589151916 19.999587815696856 18.372304458246237 8 26.923911170573984 34.20057908731245 19.247152708094724 19.628357034018844 9 26.317596114115588 16.84137762805502 20.08638301854728 36.75464323928211 10-11 25.23895709262761 26.43572745968449 26.462519439388803 21.862796008299096 12-13 26.370707075143564 23.7041618113946 27.051655815295483 22.873475298166355 14-15 23.010530466084237 25.056755592066533 25.701199101775924 26.231514840073306 16-17 22.20854093175951 28.259727465090183 26.68672067181987 22.845010931330435 18-19 22.294288781052764 26.397194984460313 29.337268454623068 21.97124777986385 20-21 22.737150407741495 25.528719279177498 29.821821510006785 21.912308803074218 22-23 22.554557150540976 25.39449874182048 29.827875365899146 22.223068741739397 24-25 22.49315964972947 25.620741621172726 29.719140632060697 22.166958097037107 26-27 21.869573899372664 26.011174282931144 29.62955454575249 22.489697271943704 28-29 22.115360250770227 26.406283310488636 29.398859600904238 22.0794968378369 30-31 22.175194545923244 25.91130739793117 29.326355706269357 22.587142349876228 32-33 21.902848835461093 25.80479829828697 29.425195475027206 22.867157391224733 34-35 22.73040746313491 25.966520775250746 29.090858713968238 22.212213047646113 36-37 22.809168873023804 25.510635196043268 29.11305203342705 22.56714389750588 38-39 22.23116485248968 25.962404123656302 29.04316840769204 22.76326261616198 40-41 22.76815928534333 25.75345858566882 29.124090772858697 22.354291356129156 42-43 22.473140895525916 25.796886970874162 28.841124353071258 22.88884778052866 44-45 22.39537782709153 26.093768239606707 29.06231253590906 22.448541397392706 46-47 22.948905523794274 26.365897081278533 28.205067131151157 22.480130263776037 48-49 22.635624683893493 26.475881643642346 28.03267134957587 22.85582232288829 50-51 22.814164679791922 26.695403570664777 28.02559326666534 22.464838482877965 52-53 23.184421055049917 26.38265028425513 27.930317878561723 22.502610782133228 54-55 22.6723665223909 26.661415258454763 28.013025023979328 22.65319319517501 56-57 22.700902295241924 26.72279131257242 27.84359495047115 22.73271144171451 58-59 22.707689365746337 26.336059943760493 27.814247454508532 23.142003235984635 60-61 22.370221913947404 26.665891850271688 28.100208761213864 22.863677474567044 62-63 22.466966975590918 26.654691924329722 28.25987950028397 22.618461599795396 64-65 22.23340713234258 26.707633957364603 28.006740902636324 23.052218007656492 66-67 22.19213987519927 26.685053931151987 27.964633881206698 23.158172312442048 68-69 22.672089535446688 26.37399820461509 28.11240312303725 22.84150913690097 70-71 22.588206596232702 26.54867271586548 27.586380484627384 23.276740203274432 72-73 22.58738648035029 26.639970403805613 27.5975390561652 23.1751040596789 74-75 22.43645858003859 27.169415519279582 27.365101706476803 23.02902419420503 76-77 22.628056709802994 26.650958615682395 27.779735465519938 22.94124920899467 78-79 22.59819024062441 26.881393291058544 27.719411279186957 22.80100518913009 80-81 22.80130628815033 26.96031909865307 27.35452999607749 22.883844617119117 82-83 22.84580709551299 26.9622026727608 27.399751198228522 22.79223903349769 84-85 22.644389608296578 27.185113219076158 27.38263146949558 22.78786570313169 86-87 22.900711647221915 27.097886208747823 27.10089321642718 22.90050892760308 88-89 22.736998372547713 27.281752634685446 26.796844830516235 23.18440416225061 90-91 22.71111967037812 27.15599770926985 27.081684125882038 23.051198494469983 92-93 22.79247432547897 26.828991827601552 27.15645874220946 23.222075104710015 94-95 22.589760733769147 26.635501704314525 27.300284035527582 23.47445352638875 96-97 22.842159906927417 26.327888276412192 27.343297721617095 23.486654095043296 98-99 22.47950143267831 26.68952487650519 26.959201524676494 23.87177216614001 100-101 22.953310667701405 25.961985795858627 27.170513551234677 23.914189985205287 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1255.0 1 835.0 2 600.5 3 777.0 4 639.0 5 606.0 6 1183.0 7 2912.0 8 4815.5 9 5469.5 10 4534.0 11 3339.0 12 3261.0 13 3505.5 14 3678.5 15 3923.0 16 4186.0 17 4423.0 18 4735.0 19 5222.0 20 5903.5 21 6884.0 22 8217.0 23 9965.5 24 12111.5 25 14976.0 26 18945.0 27 23379.5 28 26511.5 29 29480.0 30 33587.0 31 39111.5 32 46800.5 33 50264.5 34 50326.5 35 53282.5 36 59223.0 37 65089.0 38 69654.0 39 73070.5 40 76973.0 41 82333.0 42 84873.0 43 87624.0 44 91992.5 45 97606.0 46 101133.5 47 104975.5 48 108175.5 49 106361.0 50 104645.5 51 103042.0 52 102566.0 53 105412.0 54 109244.0 55 105658.5 56 97746.5 57 89624.0 58 83256.5 59 75429.0 60 61639.0 61 51309.0 62 44204.5 63 33415.5 64 23078.0 65 17169.5 66 13081.0 67 10141.0 68 7864.0 69 6153.5 70 4756.0 71 3340.5 72 2462.5 73 1972.0 74 1419.5 75 1060.5 76 786.0 77 483.0 78 306.5 79 198.5 80 117.5 81 62.0 82 36.5 83 21.0 84 15.5 85 11.5 86 7.0 87 2.0 88 0.5 89 0.5 90 1.0 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.015440018568565 3 0.002398777501899595 4 0.0018244223253884246 5 0.11433046572434129 6 0.0 7 0.0 8 3.716415848013458E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 1.689279930915208E-5 16-17 0.0 18-19 0.0 20-21 0.0 22-23 3.0407038756473744E-4 24-25 4.898911799654103E-4 26-27 0.0063516925402411824 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.01128438993851359 36-37 7.601759689118436E-4 38-39 0.007922722875992326 40-41 0.01087896275509394 42-43 0.006199657346458814 44-45 0.003631951851467697 46-47 1.689279930915208E-5 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 1.689279930915208E-5 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 8.44639965457604E-5 68-69 0.0010135679585491248 70-71 0.0 72-73 3.378559861830416E-5 74-75 0.0 76-77 0.0 78-79 0.0 80-81 1.3514239447321664E-4 82-83 0.0018582079240067285 84-85 0.0038684510417958265 86-87 0.0029731326784107662 88-89 0.0 90-91 0.0029055614811741575 92-93 0.0 94-95 0.0 96-97 1.689279930915208E-5 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2959841.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.99313386007228 #Duplication Level Percentage of deduplicated Percentage of total 1 71.28172331766679 32.784698423260835 2 14.951711049384315 13.753520954629089 3 5.4804650706015225 7.561912908228788 4 2.5995347472645074 4.782429984193826 5 1.4275553515751578 3.2828872188829386 6 0.8973159249979207 2.476222287192237 7 0.6172849030946338 1.9873607024483264 8 0.4317064262957542 1.588442516229924 9 0.3154270009609215 1.305672865044913 >10 1.7907428783110766 15.141476506847695 >50 0.13445087882209752 4.270550327361511 >100 0.06445480752585761 5.4878790160548805 >500 0.00366713629797224 1.1942021469310253 >1k 0.0037404790238509426 3.208511705713066 >5k 7.334272595786162E-5 0.2671286029431617 >10k+ 1.4668545191572324E-4 0.907103834037702 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14663 0.4953982325401939 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12228 0.4131302999046233 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7919 0.26754815545835064 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 4181 0.1412575878231297 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 3696 0.12487157249325216 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 3643 0.12308093576648205 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 3599 0.12159436942727667 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 3409 0.11517510568979888 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3244 0.10960048191777869 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 3.378559861830416E-5 2 0.0 0.0 0.0 0.0 3.378559861830416E-5 3 0.0 0.0 0.0 0.0 3.378559861830416E-5 4 0.0 0.0 0.0 0.0 3.378559861830416E-5 5 6.757119723660832E-5 0.0 0.0 0.0 3.378559861830416E-5 6 6.757119723660832E-5 0.0 0.0 0.0 6.757119723660832E-5 7 1.0135679585491247E-4 0.0 0.0 0.0 6.757119723660832E-5 8 1.0135679585491247E-4 0.0 0.0 0.0 6.757119723660832E-5 9 1.0135679585491247E-4 0.0 0.0 0.0 6.757119723660832E-5 10-11 1.0135679585491247E-4 0.0 0.0 0.0 6.757119723660832E-5 12-13 1.0135679585491247E-4 0.0 0.0 0.0 2.364991903281291E-4 14-15 1.3514239447321664E-4 0.0 0.0 0.0 2.8717758825558536E-4 16-17 1.3514239447321664E-4 0.0 0.0 3.378559861830416E-5 3.378559861830416E-4 18-19 1.5203519378236872E-4 0.0 0.0 1.0135679585491247E-4 3.716415848013457E-4 20-21 1.689279930915208E-4 0.0 0.0 1.8582079240067286E-4 4.729983806562582E-4 22-23 1.689279930915208E-4 0.0 0.0 2.8717758825558536E-4 5.574623772020186E-4 24-25 2.0271359170982495E-4 0.0 0.0 4.5610558134710614E-4 6.081407751294749E-4 26-27 2.702847889464333E-4 0.0 0.0 0.0011656031523314936 6.588191730569311E-4 28-29 2.702847889464333E-4 0.0 0.0 0.0030744894742656785 6.757119723660832E-4 30-31 2.702847889464333E-4 0.0 0.0 0.010017429990327183 6.757119723660832E-4 32-33 3.2096318687388953E-4 0.0 0.0 0.023835739825213587 6.757119723660832E-4 34-35 3.716415848013457E-4 0.0 0.0 0.04420845579205099 7.432831696026914E-4 36-37 4.054271834196499E-4 0.0 0.0 0.0771325216455884 7.432831696026914E-4 38-39 4.054271834196499E-4 0.0 0.0 0.12727034999515177 7.770687682209956E-4 40-41 4.054271834196499E-4 0.0 0.0 0.17945220706112253 7.770687682209956E-4 42-43 4.3921278203795406E-4 0.0 0.0 0.23433691201655765 7.770687682209956E-4 44-45 4.729983806562582E-4 0.0 0.0 0.3029385700110242 7.939615675301477E-4 46-47 5.236767785837145E-4 0.0 0.0 0.3832300451274241 8.277471661484519E-4 48-49 5.405695778928666E-4 0.0 0.0 0.4628458082714578 8.784255640759081E-4 50-51 5.405695778928666E-4 0.0 0.0 0.5519553246272351 9.459967613125164E-4 52-53 5.405695778928666E-4 0.0 0.0 0.6362841787785223 9.797823599308206E-4 54-55 5.405695778928666E-4 0.0 0.0 0.7274884022486343 9.797823599308206E-4 56-57 5.405695778928666E-4 0.0 0.0 0.8357374602216808 0.001030460757858277 58-59 5.405695778928666E-4 0.0 0.0 0.9480745756275422 0.0010811391557857331 60-61 5.405695778928666E-4 0.0 0.0 1.0703615498264942 0.0010811391557857331 62-63 5.405695778928666E-4 0.0 0.0 1.188307074602994 0.0011487103530223414 64-65 5.405695778928666E-4 0.0 0.0 1.3093946600509958 0.0011824959516406456 66-67 5.405695778928666E-4 0.0 0.0 1.4534226669608268 0.0012162815502589498 68-69 5.405695778928666E-4 0.0 0.0 1.6115561612937992 0.0012162815502589498 70-71 5.405695778928666E-4 0.0 0.0 1.7766494889421425 0.0012838527474955581 72-73 5.405695778928666E-4 0.0 0.0 1.9465403715942848 0.0014189951419687746 74-75 5.405695778928666E-4 0.0 0.0 2.1212963804474634 0.0015710303357511432 76-77 5.405695778928666E-4 1.689279930915208E-5 0.0 2.309684878343127 0.0015879231350602954 78-79 5.405695778928666E-4 3.378559861830416E-5 0.0 2.5165034202850762 0.0016217087336785996 80-81 6.419263737477791E-4 3.378559861830416E-5 0.0 2.73955594236312 0.0016554943322969037 82-83 6.419263737477791E-4 6.757119723660832E-5 0.0 2.970142652933046 0.0017568511281518162 84-85 6.588191730569311E-4 6.757119723660832E-5 0.0 3.2046484929426953 0.0017568511281518162 86-87 6.757119723660832E-4 6.757119723660832E-5 0.0 3.4530571067837768 0.001891993522625033 88-89 6.757119723660832E-4 6.757119723660832E-5 0.0 3.7065166676182946 0.0020440287164074015 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7160 0.0 51.088585 1 GTATCAA 12450 0.0 42.14215 1 TATCAAC 16115 0.0 32.439785 2 ATCAACG 16090 0.0 32.334164 3 TCAACGC 16260 0.0 32.02427 4 CAACGCA 16520 0.0 31.489872 5 AACGCAG 17025 0.0 30.574438 6 ACGCAGA 19290 0.0 26.861334 7 CGCAGAG 19380 0.0 26.516033 8 TAAGGTG 2120 0.0 25.79441 5 GTAAGGT 2185 0.0 24.375427 4 GCAGAGT 21030 0.0 24.187181 9 TGGTATC 3520 0.0 23.378355 2 GAGTACT 13025 0.0 23.300121 12-13 AGGTAAG 2215 0.0 23.193193 2 AAGGTAA 2210 0.0 22.599955 1 GGTAAGG 2360 0.0 22.16568 3 AGAGTAC 18365 0.0 22.059374 10-11 GTACTTT 13665 0.0 21.878683 14-15 AGTACTT 13450 0.0 21.875303 12-13 >>END_MODULE