FastQCFastQC Report
Thu 26 May 2016
SRR938290_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938290_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2959980
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT151790.5128075189697228No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT123640.4177055250373313No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77470.26172474138338775No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC39110.13212927114372394No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA36540.12344678004581112No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA35190.11888593841850283No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA34980.11817647416536597No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32270.10902100689869525No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA32000.10810883857323361No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA31030.10483178940398245No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA74850.051.269661
GTATCAA131200.041.545931
TATCAAC167950.032.315652
ATCAACG169700.031.9269283
TCAACGC170150.031.8424914
CAACGCA172400.031.2615075
AACGCAG179000.030.2385356
ACGCAGA200200.027.025037
CGCAGAG200600.026.8764218
TGGTATC36400.025.9823512
GCAGAGT216100.024.4866629
GTGGTAT39150.024.383411
TAAGGTG20450.024.1698595
AGGTAAG17550.023.5596542
GGTAAGG19450.023.4577183
GAGTACT135100.023.29303212-13
GTAAGGT19950.022.8698044
GTACTTT140150.022.03192514-15
AGAGTAC190350.021.7095510-11
GTACATG111200.021.6332591
Produced by FastQC (version 0.11.5)