##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938290_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2959980 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.758563909215603 33.0 31.0 34.0 30.0 34.0 2 31.964612260893656 33.0 31.0 34.0 30.0 34.0 3 32.043394212123054 34.0 31.0 34.0 30.0 34.0 4 35.575560983520155 37.0 35.0 37.0 33.0 37.0 5 35.57875289697903 37.0 35.0 37.0 33.0 37.0 6 35.603371982243125 37.0 35.0 37.0 33.0 37.0 7 35.5410036554301 37.0 35.0 37.0 33.0 37.0 8 35.51126561665957 37.0 35.0 37.0 33.0 37.0 9 37.12613767660592 39.0 37.0 39.0 34.0 39.0 10-11 37.031266934236044 39.0 37.0 39.0 33.0 39.0 12-13 36.92156281461361 39.0 37.0 39.0 33.0 39.0 14-15 38.0707040250272 40.0 38.0 41.0 33.0 41.0 16-17 37.90729295468213 40.0 38.0 41.0 33.0 41.0 18-19 38.065030169122764 40.0 38.0 41.0 33.0 41.0 20-21 38.04133355630782 40.0 38.0 41.0 33.0 41.0 22-23 37.942882553260496 40.0 38.0 41.0 33.0 41.0 24-25 37.8231793795904 40.0 38.0 41.0 32.5 41.0 26-27 37.6289537429307 40.0 37.5 41.0 32.0 41.0 28-29 37.40374124149487 40.0 37.0 41.0 31.5 41.0 30-31 37.1554801045953 40.0 37.0 41.0 31.0 41.0 32-33 36.86662646369232 39.5 36.0 41.0 30.5 41.0 34-35 36.60733281981635 39.0 35.5 41.0 30.0 41.0 36-37 36.31165430171826 39.0 35.0 41.0 30.0 41.0 38-39 35.98310140609058 39.0 35.0 40.0 29.0 41.0 40-41 35.729435502942586 39.0 35.0 40.0 27.5 41.0 42-43 35.502655592267516 39.0 35.0 40.0 26.5 41.0 44-45 35.18148129379253 38.0 35.0 40.0 25.5 41.0 46-47 34.74913546713154 38.0 34.0 40.0 24.0 41.0 48-49 34.30065422739342 38.0 33.5 40.0 22.5 41.0 50-51 33.58506814235231 37.5 33.0 39.5 21.0 40.5 52-53 33.70441269873445 38.0 33.0 39.5 22.0 40.5 54-55 33.778783302589886 38.0 33.0 40.0 20.5 41.0 56-57 33.43817035925919 37.5 33.0 40.0 18.5 41.0 58-59 33.08174396448625 37.0 33.0 40.0 16.0 41.0 60-61 32.62033915769701 36.5 32.0 39.5 11.0 41.0 62-63 32.035046689504654 36.0 31.0 39.0 7.5 40.5 64-65 31.471927681943797 35.0 31.0 39.0 4.5 40.0 66-67 30.90501000006757 35.0 30.0 38.0 2.0 40.0 68-69 30.260408347353696 35.0 29.0 38.0 2.0 40.0 70-71 29.662103797998633 34.0 28.5 37.0 2.0 39.0 72-73 28.97522550828046 34.0 27.5 36.5 2.0 39.0 74-75 28.249074149149656 34.0 26.0 36.0 2.0 39.0 76-77 27.547435793485093 33.0 25.5 35.0 2.0 37.5 78-79 26.782523023804217 33.0 24.5 35.0 2.0 37.0 80-81 26.092270724802198 32.0 22.0 35.0 2.0 36.5 82-83 25.32083645835445 31.5 19.0 35.0 2.0 36.0 84-85 24.619140500949328 31.0 16.0 34.0 2.0 35.5 86-87 23.888030155609158 31.0 7.5 34.0 2.0 35.0 88-89 23.026482104608817 30.0 2.0 34.0 2.0 35.0 90-91 22.133840093514145 29.5 2.0 34.0 2.0 35.0 92-93 21.192302819613644 29.0 2.0 34.0 2.0 35.0 94-95 20.182091433050225 28.0 2.0 34.0 2.0 35.0 96-97 18.936289603308133 25.5 2.0 33.0 2.0 35.0 98-99 17.420960276758628 21.5 2.0 32.5 2.0 34.0 100-101 13.9639781349874 2.0 2.0 28.5 2.0 32.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.40541216667534385 1101 2 0.3514527586392333 1101 3 0.33079436818136543 1101 4 0.290860562666694 1101 5 0.3047861824223048 1101 6 0.24918802292785358 1101 7 0.30311551205327447 1101 8 0.25834921990797 1101 9 0.4181668556258842 1101 10-11 0.42159884427771743 1101 12-13 0.5327936609372088 1101 14-15 0.6691390657622094 1101 16-17 0.7611359128894932 1101 18-19 0.574501570608426 1101 20-21 0.6054895362004729 1101 22-23 0.6235104239331761 1101 24-25 0.6104299419221917 1101 26-27 0.725482911340201 1101 28-29 0.762674129795279 1101 30-31 0.7334691573303687 1101 32-33 0.7556201107777198 1101 34-35 0.6996882496802215 1101 36-37 0.8547513532042146 1101 38-39 0.9780275604870567 1101 40-41 1.109005183652549 1101 42-43 1.3624441357277135 1101 44-45 1.2652209359270898 1101 46-47 1.3845453486959158 1101 48-49 1.4158079343692123 1101 50-51 1.3410825841157958 1101 52-53 1.37853141231313 1101 54-55 1.540136656900998 1101 56-57 1.5257005792257772 1101 58-59 1.6366493862248603 1101 60-61 1.6437269892944997 1101 62-63 1.6495915968488006 1101 64-65 1.5524327456850777 1101 66-67 1.750245519720739 1101 68-69 2.020307251627269 1101 70-71 2.2748396785424845 1101 72-73 2.4702774897142703 1101 74-75 2.6998482680657183 1101 76-77 2.8084512530137395 1101 78-79 2.8537114623451814 1101 80-81 3.117832709008937 1101 82-83 3.2460833934593865 1101 84-85 3.2948124394650264 1101 86-87 3.6594436304377957 1101 88-89 4.043985886052166 1101 90-91 4.210840351057065 1101 92-93 4.523767626401 1101 94-95 4.810252325082114 1101 96-97 5.0345895896567505 1101 98-99 5.3024148981182755 1101 100-101 5.054072900709508 1102 1 0.35882868112209465 1102 2 0.30503274267673675 1102 3 0.2526759942282695 1102 4 0.21981792153000157 1102 5 0.237468508830176 1102 6 0.35971312309814607 1102 7 0.1172886001889708 1102 8 0.2089141945174262 1102 9 0.2375141682349522 1102 10-11 0.2733736239406568 1102 12-13 0.3514362187058637 1102 14-15 0.420762632440308 1102 16-17 0.49256975655821833 1102 18-19 0.34827215932153166 1102 20-21 0.41828050412257056 1102 22-23 0.4815018177145092 1102 24-25 0.35946451740279173 1102 26-27 0.3655534417477142 1102 28-29 0.47312135426663815 1102 30-31 0.5650305101705868 1102 32-33 0.4761628471632733 1102 34-35 0.5342082431240911 1102 36-37 0.6463429457506962 1102 38-39 0.7117550371669807 1102 40-41 0.7392646479059053 1102 42-43 0.8040574519518984 1102 44-45 0.8253265797761458 1102 46-47 0.9242587031063749 1102 48-49 1.1050335250060925 1102 50-51 1.036355248791537 1102 52-53 1.0312013867216763 1102 54-55 1.0668257053634491 1102 56-57 1.154538465088443 1102 58-59 1.1694513446223525 1102 60-61 1.3096695092815835 1102 62-63 1.2084160784941034 1102 64-65 1.3445990111695245 1102 66-67 1.372283374132497 1102 68-69 1.5313205636310272 1102 70-71 1.763986656349644 1102 72-73 2.0827667543439325 1102 74-75 1.9073583877150462 1102 76-77 2.164747462802893 1102 78-79 2.4712239841736476 1102 80-81 2.483346883744776 1102 82-83 2.787709096371401 1102 84-85 2.948814813893353 1102 86-87 3.11983846080955 1102 88-89 3.351186511948466 1102 90-91 3.48614583395144 1102 92-93 3.645801722891129 1102 94-95 4.176452826388857 1102 96-97 4.262919462329695 1102 98-99 4.424119453788851 1102 100-101 4.175108401795116 1103 1 0.6852725740405283 1103 2 0.5628867182052204 1103 3 0.5843120283271972 1103 4 0.48112256011189913 1103 5 0.5080325499474938 1103 6 0.552122740820657 1103 7 0.6355672245793542 1103 8 0.5979740990775682 1103 9 0.7615283678244253 1103 10-11 0.7649390435739747 1103 12-13 0.8404151547929075 1103 14-15 1.0195843679172825 1103 16-17 1.1457000777881063 1103 18-19 0.9805807666032464 1103 20-21 1.110532272007113 1103 22-23 1.1327564422017744 1103 24-25 1.1533209546150616 1103 26-27 1.2090743828792228 1103 28-29 1.2178417523341523 1103 30-31 1.2370272088635943 1103 32-33 1.2398348128302175 1103 34-35 1.4322436391631967 1103 36-37 1.4071068414140555 1103 38-39 1.5084572285970381 1103 40-41 1.6328751185725778 1103 42-43 1.6925925079032353 1103 44-45 1.675265878650393 1103 46-47 1.8859827664521802 1103 48-49 1.9595325565464563 1103 50-51 1.9115311794580307 1103 52-53 1.9837160851738247 1103 54-55 2.029523862834509 1103 56-57 2.0462317792521745 1103 58-59 2.283613191416684 1103 60-61 2.279791823143235 1103 62-63 2.513162724878839 1103 64-65 2.444941638665437 1103 66-67 2.5603896705665896 1103 68-69 2.7195894193281838 1103 70-71 2.9717184727547235 1103 72-73 3.1003901799662934 1103 74-75 3.1726175978368154 1103 76-77 3.4687521224426483 1103 78-79 3.6058104394633723 1103 80-81 3.807808529177546 1103 82-83 3.893273046664092 1103 84-85 3.935899383731101 1103 86-87 4.0108662322894055 1103 88-89 4.368537043205254 1103 90-91 4.673684055981997 1103 92-93 4.867458972950164 1103 94-95 5.239679633647562 1103 96-97 5.51410531239102 1103 98-99 5.504615386527494 1103 100-101 5.158767033061254 1104 1 0.3422836505135045 1104 2 0.29463283109461713 1104 3 0.27618444168430045 1104 4 0.30648601349832916 1104 5 0.3705226661811878 1104 6 0.30807069609854665 1104 7 0.2723803292888931 1104 8 0.33788131418159395 1104 9 0.42069905101576666 1104 10-11 0.39996444946630305 1104 12-13 0.35352857596446796 1104 14-15 0.4780273276205591 1104 16-17 0.602109870384794 1104 18-19 0.5597721943605549 1104 20-21 0.48367908473068866 1104 22-23 0.5083610383119108 1104 24-25 0.5555998471250518 1104 26-27 0.6155883217877189 1104 28-29 0.7251841731868893 1104 30-31 0.6365607283676553 1104 32-33 0.5668276130320393 1104 34-35 0.5449259859549898 1104 36-37 0.5822722867386005 1104 38-39 0.5607654169835925 1104 40-41 0.7060616478536303 1104 42-43 0.7410265170129335 1104 44-45 0.7485724953139226 1104 46-47 0.7923865244310306 1104 48-49 0.8599372389259159 1104 50-51 0.9438666733559629 1104 52-53 0.9486567704765179 1104 54-55 1.0762833789277337 1104 56-57 1.2339419699823964 1104 58-59 1.2421068177107983 1104 60-61 1.2040187265887496 1104 62-63 1.3522541776215817 1104 64-65 1.4927224268621408 1104 66-67 1.6031459966307544 1104 68-69 1.7199047621398513 1104 70-71 1.867684156802099 1104 72-73 2.025852894497717 1104 74-75 2.102019980104295 1104 76-77 2.235745545767916 1104 78-79 2.395247498853088 1104 80-81 2.557133907301555 1104 82-83 2.9177389602991823 1104 84-85 2.966345770008349 1104 86-87 3.0309403355814943 1104 88-89 3.089391049011798 1104 90-91 3.4477995383007176 1104 92-93 3.76349301908429 1104 94-95 3.8799865400937428 1104 96-97 4.030678807986504 1104 98-99 4.189601453971232 1104 100-101 4.055288310307029 1105 1 0.20839010757579146 1105 2 0.23791041266032664 1105 3 0.07809513353299025 1105 4 0.19005176957570313 1105 5 0.1786987542735332 1105 6 0.22418846951025984 1105 7 0.21546860262355239 1105 8 0.25155197686177644 1105 9 0.2260739012464157 1105 10-11 0.21085965073045543 1105 12-13 0.2253435532525998 1105 14-15 0.4027643776903105 1105 16-17 0.470270128117356 1105 18-19 0.3971121152862125 1105 20-21 0.4441675979241211 1105 22-23 0.4235167796421493 1105 24-25 0.38699324568961657 1105 26-27 0.34632959791883167 1105 28-29 0.538533775030217 1105 30-31 0.5181101011958731 1105 32-33 0.5087757655853196 1105 34-35 0.45807662051913667 1105 36-37 0.5074020203983238 1105 38-39 0.5526120641035988 1105 40-41 0.6991802025319842 1105 42-43 0.7738159100112441 1105 44-45 0.8181436688871315 1105 46-47 0.8497140571718447 1105 48-49 0.870490888047101 1105 50-51 0.9294897839043372 1105 52-53 0.9168156271914967 1105 54-55 1.1066076643831266 1105 56-57 1.1223917531265144 1105 58-59 1.1144603894456893 1105 60-61 1.2403718869028637 1105 62-63 1.3686242139329963 1105 64-65 1.374174525785829 1105 66-67 1.5149102502477412 1105 68-69 1.6686936393814413 1105 70-71 1.7979973708013866 1105 72-73 1.9820806625980971 1105 74-75 2.036277172535655 1105 76-77 2.1277904197919426 1105 78-79 2.3702416128706325 1105 80-81 2.447376024783601 1105 82-83 2.6122771444641977 1105 84-85 2.6599225485631663 1105 86-87 2.9514799141576837 1105 88-89 3.3741056167357932 1105 90-91 3.488751168680839 1105 92-93 3.652145892827658 1105 94-95 3.9332475907908737 1105 96-97 3.9506218719983117 1105 98-99 3.9310822133865493 1105 100-101 3.899406593552465 1106 1 0.08908475938690685 1106 2 0.09182150165232628 1106 3 0.05464284294819066 1106 4 0.06778480642357465 1106 5 0.13579460431488855 1106 6 -0.12787975119155703 1106 7 -0.0026421459798982028 1106 8 0.11040523499013233 1106 9 0.05384332850106688 1106 10-11 0.04478964150857223 1106 12-13 0.2897442398387611 1106 14-15 0.436362581797205 1106 16-17 0.15365582677127065 1106 18-19 0.21785978593710809 1106 20-21 0.14772117839942922 1106 22-23 0.11150224805621889 1106 24-25 0.08839713898836266 1106 26-27 0.1676366698344296 1106 28-29 0.20264210952257145 1106 30-31 0.1277040423467426 1106 32-33 0.20055773370586394 1106 34-35 0.38310635005714744 1106 36-37 0.3671586990014859 1106 38-39 0.12400412144347683 1106 40-41 0.4642153613064366 1106 42-43 0.3350121182593284 1106 44-45 0.438713325376213 1106 46-47 0.6855019415961365 1106 48-49 0.6767243999531587 1106 50-51 0.5965275601069635 1106 52-53 0.6709589109970011 1106 54-55 0.2197301065117685 1106 56-57 0.6680997797387107 1106 58-59 0.7491833727105686 1106 60-61 0.9572273200771733 1106 62-63 0.728012966811697 1106 64-65 0.8728997164933467 1106 66-67 1.0395646810241352 1106 68-69 0.792329920756643 1106 70-71 1.1075624342234676 1106 72-73 1.4611302334258944 1106 74-75 1.5943626293874722 1106 76-77 1.7308232515911826 1106 78-79 2.0255387211319515 1106 80-81 2.2717683217471922 1106 82-83 2.310963515014052 1106 84-85 2.48260636759532 1106 86-87 2.419988331987696 1106 88-89 2.6812765656289166 1106 90-91 2.973779434782685 1106 92-93 2.9614145824993194 1106 94-95 3.1246150489977644 1106 96-97 3.2985902372004823 1106 98-99 3.6701285333752445 1106 100-101 3.5999499081906787 1107 1 0.29911528479868466 1107 2 0.21397278792026242 1107 3 0.10380290040151152 1107 4 0.0458919106407123 1107 5 0.22405825334791274 1107 6 0.18960299920195922 1107 7 0.2422430728140199 1107 8 0.24554223105262452 1107 9 0.16497781805309586 1107 10-11 0.19103241113859326 1107 12-13 0.23081642424448034 1107 14-15 0.28702435713892527 1107 16-17 0.36911906417293494 1107 18-19 0.28837786393035714 1107 20-21 0.354577039636105 1107 22-23 0.35506765244720384 1107 24-25 0.43147287713060933 1107 26-27 0.5177883895109119 1107 28-29 0.48007219971104576 1107 30-31 0.5933521989460004 1107 32-33 0.5635449647003412 1107 34-35 0.644878649380189 1107 36-37 0.6775015240885836 1107 38-39 0.7314795113643839 1107 40-41 0.8099761507504795 1107 42-43 0.9186564969452391 1107 44-45 0.8469665021487174 1107 46-47 0.9524502704379287 1107 48-49 0.9328925987617112 1107 50-51 0.7749537960552146 1107 52-53 0.7783302721933865 1107 54-55 0.8755785330839814 1107 56-57 0.9176698672904351 1107 58-59 1.0248464157188906 1107 60-61 1.005726696385203 1107 62-63 1.162371001762999 1107 64-65 1.3575891862137333 1107 66-67 1.4444240340818304 1107 68-69 1.5107567758599494 1107 70-71 1.6655596490932254 1107 72-73 1.9090209162631844 1107 74-75 2.1059208665249223 1107 76-77 2.3060755706204326 1107 78-79 2.365787909691889 1107 80-81 2.465119290324541 1107 82-83 2.625028422746606 1107 84-85 2.8442402644131306 1107 86-87 2.9759918675765675 1107 88-89 3.2992299272405567 1107 90-91 3.5565273730230373 1107 92-93 3.7356139643050206 1107 94-95 4.037984848428888 1107 96-97 4.1950292949959405 1107 98-99 4.0360625688090614 1107 100-101 3.8090989916543823 1108 1 0.13921728896229624 1108 2 0.056343513730531924 1108 3 0.0398120112178475 1108 4 0.10211581678412074 1108 5 0.13915846859337222 1108 6 0.1611398731701783 1108 7 0.1213075629854643 1108 8 0.14240890431987197 1108 9 0.1343146002389588 1108 10-11 0.1072682487857719 1108 12-13 0.13300832081362302 1108 14-15 0.20271317439706849 1108 16-17 0.23573929466230226 1108 18-19 0.22942925124774405 1108 20-21 0.12113523074665977 1108 22-23 0.12638643483634127 1108 24-25 0.1606595335068306 1108 26-27 0.07927696319359967 1108 28-29 0.22272030119201247 1108 30-31 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-2.9966019499573022 2316 84-85 -3.091221053775719 2316 86-87 -2.9178542124251017 2316 88-89 -3.346233617089279 2316 90-91 -3.4866299991147933 2316 92-93 -3.9674875399791283 2316 94-95 -4.0102629300679205 2316 96-97 -4.280215603025731 2316 98-99 -4.125169969447331 2316 100-101 -3.6250420412210023 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 9163.0 3 2947.0 4 3114.0 5 3463.0 6 4037.0 7 5079.0 8 6455.0 9 8194.0 10 10106.0 11 12480.0 12 15612.0 13 19082.0 14 22411.0 15 27126.0 16 32627.0 17 39941.0 18 38265.0 19 33303.0 20 33095.0 21 35024.0 22 38385.0 23 42314.0 24 47186.0 25 52978.0 26 59838.0 27 68231.0 28 77952.0 29 91165.0 30 105589.0 31 126419.0 32 152299.0 33 189585.0 34 242930.0 35 318114.0 36 394549.0 37 398652.0 38 182767.0 39 9503.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.54353426873377 18.545630044498328 15.154274551037316 22.756561135730585 2 15.609629812633477 19.6804302207646 40.15241716976139 24.557522796840534 3 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22.96030531487751 26.39132974415669 28.193724297097045 22.454640643868753 48-49 22.642632251070474 26.484902512536323 28.029474848109338 22.842990388283866 50-51 22.834196962096918 26.662535350042077 28.02693420208119 22.47633348577982 52-53 23.09251169198113 26.407540174765593 27.973763873510016 22.52618425974326 54-55 22.58446283270434 26.646335509040895 28.118291723221883 22.650909935032878 56-57 22.683352038515725 26.73722093644625 27.846658164157446 22.73276886088058 58-59 22.67526989843679 26.299594993961144 27.88226594568733 23.142869161914735 60-61 22.319477534602893 26.599852610586833 28.196351622077596 22.88431823273268 62-63 22.419362577640275 26.616414629316886 28.36001367806934 22.604209114973496 64-65 22.185507777218746 26.63168188162976 28.131490800570507 23.051319540580987 66-67 22.191303125608698 26.622586681636673 28.050858610286095 23.135251582468534 68-69 22.671380537022394 26.295611839907934 28.225985337040772 22.8070222860289 70-71 22.60844281547024 26.49685094897284 27.670813928067357 23.223892307489567 72-73 22.58092092481057 26.554886341558188 27.69354879583717 23.170643937794072 74-75 22.47176774583749 26.998169517697058 27.54949355530008 22.980569181165375 76-77 22.805046325672418 26.5734713474145 27.63829282582209 22.98318950109099 78-79 22.833011975201163 26.77606189673549 27.525713578083604 22.865212549979734 80-81 23.044389854905965 26.861995093699527 27.15356855642583 22.940046494968676 82-83 23.030217636384492 26.825461265771633 27.20502955435909 22.93929154348479 84-85 22.90526133688021 27.044672767379506 27.178981480433418 22.871084415306868 86-87 23.193349707926362 26.918834273126862 26.92802386591303 22.95979215303375 88-89 23.081960875489326 27.00737166663062 26.706374899971884 23.20429255790817 90-91 23.08909550695266 26.989340954613304 26.721014584585834 23.2005489538482 92-93 23.232892875751226 26.498412906513195 26.94494133802422 23.323752879711357 94-95 23.092742777619694 26.327783834493722 27.087922337656543 23.49155105023004 96-97 23.377588883806837 25.994651621232595 27.04438965137539 23.58336984358518 98-99 23.1771799119562 26.29204826442539 26.641199125813426 23.889572697804986 100-101 24.13136844783474 25.1124127342228 26.76123433749737 23.99498448044509 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1258.0 1 858.5 2 626.5 3 784.0 4 657.0 5 616.0 6 1145.0 7 2739.0 8 4712.5 9 5448.0 10 4490.0 11 3321.0 12 3163.5 13 3379.0 14 3581.5 15 3846.5 16 4125.0 17 4410.5 18 4815.0 19 5256.5 20 5883.5 21 7016.5 22 8433.0 23 10127.0 24 12304.0 25 15231.0 26 19128.0 27 23534.0 28 26711.0 29 29865.0 30 33848.5 31 39038.5 32 46475.0 33 50228.0 34 50608.0 35 53537.5 36 59489.5 37 65000.5 38 69115.0 39 72830.0 40 76562.5 41 81654.5 42 84503.5 43 86457.5 44 90715.0 45 96561.5 46 99985.5 47 102941.0 48 105625.0 49 104955.0 50 103844.0 51 101893.5 52 101605.5 53 104663.5 54 108425.5 55 105390.5 56 97671.0 57 90146.5 58 84328.5 59 76277.0 60 62648.5 61 52558.5 62 45057.5 63 34500.0 64 24908.5 65 18896.0 66 14217.0 67 10996.5 68 8615.5 69 6750.0 70 5339.5 71 3884.5 72 2836.0 73 2180.0 74 1608.5 75 1189.5 76 879.5 77 592.0 78 403.5 79 250.5 80 143.0 81 95.0 82 60.5 83 38.0 84 25.0 85 18.5 86 17.0 87 12.5 88 7.0 89 6.5 90 5.5 91 5.0 92 4.5 93 3.5 94 2.5 95 3.5 96 3.5 97 1.5 98 0.5 99 1.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4586855316589977 2 0.03807458158501071 3 0.05000033784012054 4 0.05469631551564538 5 0.08010189258035527 6 0.07976405245981393 7 0.04462867992351299 8 0.04003405428415057 9 0.04341245548956412 10-11 0.047855053074682935 12-13 0.04317596740518517 14-15 0.03677389712092649 16-17 0.03721308927763026 18-19 0.04576044432732653 20-21 0.05304089892499273 22-23 0.04589558037554308 24-25 0.0628044784086379 26-27 0.05461185548551004 28-29 0.042635423212319 30-31 0.044763815971729536 32-33 0.04381786363421375 34-35 0.048429381279603244 36-37 0.04369961959202427 38-39 0.0244089487091129 40-41 0.0228379921485956 42-43 0.022196095919567022 44-45 0.015219697430388044 46-47 0.027213021709606145 48-49 0.026317745390171555 50-51 0.015929161683524887 52-53 0.015979837701606093 54-55 0.016317677822147447 56-57 0.015591321562983535 58-59 0.013327792755356456 60-61 0.015979837701606093 62-63 0.01682443800295948 64-65 0.016993358063230157 66-67 0.009611551429401548 68-69 0.011672376164703815 70-71 0.017466334231988054 72-73 0.015371725484631654 74-75 0.012753464550436152 76-77 0.009527091399266212 78-79 0.013699416887951945 80-81 0.017567686268150463 82-83 0.014645369225467738 84-85 0.012854816586598558 86-87 0.0037162413259549053 88-89 0.027432617787958028 90-91 0.03030425881255954 92-93 0.016283893810093313 94-95 0.03087858701747985 96-97 0.025861661227440727 98-99 0.02221298792559409 100-101 0.14709558848370596 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2959980.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.24187629921892 #Duplication Level Percentage of deduplicated Percentage of total 1 73.0872098072541 35.258641345766115 2 14.221700214870904 13.721630050607544 3 5.100480204110322 7.381702052199149 4 2.3986156117490607 4.62854870525494 5 1.3261483521640374 3.1987942379755245 6 0.8184363622779116 2.368974344867624 7 0.5532865657895556 1.8684107445387577 8 0.39112894065602316 1.5095035177737925 9 0.29675866797799055 1.288457545618368 >10 1.6183765055624966 14.204081867901797 >50 0.12298272286604722 4.058710065823714 >100 0.058120166732506785 5.166568756741128 >500 0.0028853228912300087 0.9957080330081254 >1k 0.003659433910708714 3.152187089725721 >5k 7.037372905209066E-5 0.26300760788059285 >10k+ 1.407474581041813E-4 0.9350740343171768 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15179 0.5128075189697228 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12364 0.4177055250373313 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7747 0.26172474138338775 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 3911 0.13212927114372394 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 3654 0.12344678004581112 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 3519 0.11888593841850283 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 3498 0.11817647416536597 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3227 0.10902100689869525 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 3200 0.10810883857323361 No Hit GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 3103 0.10483178940398245 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.35136048216542E-4 2 0.0 0.0 0.0 0.0 1.35136048216542E-4 3 0.0 0.0 0.0 0.0 1.35136048216542E-4 4 0.0 0.0 0.0 0.0 1.35136048216542E-4 5 0.0 0.0 0.0 0.0 1.689200602706775E-4 6 0.0 0.0 0.0 0.0 1.689200602706775E-4 7 0.0 0.0 0.0 0.0 1.689200602706775E-4 8 0.0 0.0 0.0 0.0 1.689200602706775E-4 9 0.0 0.0 0.0 3.37840120541355E-5 1.689200602706775E-4 10-11 0.0 0.0 0.0 5.0676018081203256E-5 1.689200602706775E-4 12-13 0.0 0.0 3.37840120541355E-5 6.7568024108271E-5 3.37840120541355E-4 14-15 3.37840120541355E-5 0.0 3.37840120541355E-5 8.446003013533875E-5 4.2230015067669374E-4 16-17 6.7568024108271E-5 0.0 3.37840120541355E-5 1.35136048216542E-4 5.912202109473713E-4 18-19 1.0135203616240651E-4 0.0 3.37840120541355E-5 2.02704072324813E-4 6.250042230015068E-4 20-21 1.35136048216542E-4 0.0 3.37840120541355E-5 2.3648808437894852E-4 6.587882350556423E-4 22-23 1.35136048216542E-4 0.0 3.37840120541355E-5 4.05408144649626E-4 7.43248265190981E-4 24-25 1.689200602706775E-4 0.0 3.37840120541355E-5 7.43248265190981E-4 7.43248265190981E-4 26-27 1.8581206629774525E-4 0.0 3.37840120541355E-5 0.0011148723977864716 7.939242832721842E-4 28-29 2.02704072324813E-4 0.0 3.37840120541355E-5 0.003868269380198515 8.10816289299252E-4 30-31 2.364880843789485E-4 0.0 3.37840120541355E-5 0.011081155953756444 8.10816289299252E-4 32-33 2.364880843789485E-4 0.0 3.37840120541355E-5 0.0254562530827911 8.446003013533875E-4 34-35 2.70272096433084E-4 0.0 3.37840120541355E-5 0.04633477253224684 8.78384313407523E-4 36-37 3.040561084872195E-4 0.0 3.37840120541355E-5 0.0785140440138109 8.78384313407523E-4 38-39 3.040561084872195E-4 0.0 3.37840120541355E-5 0.12723058939587428 9.45952337515794E-4 40-41 3.2094811451428726E-4 0.0 3.37840120541355E-5 0.17880188379651213 9.797363495699296E-4 42-43 3.8851613862255825E-4 0.0 3.37840120541355E-5 0.23515361590281014 9.797363495699296E-4 44-45 4.391921567037615E-4 0.0 3.37840120541355E-5 0.3035324563003804 0.001013520361624065 46-47 5.067601808120325E-4 0.0 3.37840120541355E-5 0.38257015250103044 0.0010979803917594037 48-49 5.40544192866168E-4 0.0 3.37840120541355E-5 0.4629254251717917 0.0011317644038135394 50-51 5.40544192866168E-4 0.0 3.37840120541355E-5 0.5511016966330853 0.001216224433948878 52-53 5.40544192866168E-4 0.0 3.37840120541355E-5 0.6364570030878587 0.001283792458057149 54-55 5.40544192866168E-4 0.0 3.37840120541355E-5 0.7307481807309508 0.0013175764701112845 56-57 6.08112216974439E-4 0.0 3.37840120541355E-5 0.8411712241298928 0.0013344684761383524 58-59 6.08112216974439E-4 0.0 3.37840120541355E-5 0.960141622578531 0.0013682524881924877 60-61 6.08112216974439E-4 0.0 3.37840120541355E-5 1.084838411070345 0.0013851444942195555 62-63 6.08112216974439E-4 0.0 3.37840120541355E-5 1.2063932864411246 0.001418928506273691 64-65 6.08112216974439E-4 0.0 3.37840120541355E-5 1.330904262866641 0.001486496530381962 66-67 6.08112216974439E-4 0.0 3.37840120541355E-5 1.4748748302353394 0.0015033885364090296 68-69 6.08112216974439E-4 0.0 3.37840120541355E-5 1.6328826546125312 0.0015202805424360975 70-71 6.587882350556423E-4 0.0 3.37840120541355E-5 1.7959749728038703 0.0015202805424360975 72-73 6.925722471097778E-4 0.0 3.37840120541355E-5 1.964658544990169 0.0015709565605173006 74-75 7.094642531368455E-4 0.0 3.37840120541355E-5 2.1339333373874148 0.0016385245846255716 76-77 7.094642531368455E-4 0.0 3.37840120541355E-5 2.3144075297806066 0.0016554165906526395 78-79 7.094642531368455E-4 1.689200602706775E-5 3.37840120541355E-5 2.5183785025574497 0.001689200602706775 80-81 7.094642531368455E-4 6.7568024108271E-5 3.37840120541355E-5 2.7354914560233516 0.0017398766207879783 82-83 7.094642531368455E-4 6.7568024108271E-5 3.37840120541355E-5 2.9580774194420236 0.001824336650923317 84-85 8.10816289299252E-4 6.7568024108271E-5 3.37840120541355E-5 3.1855113885904633 0.001824336650923317 86-87 8.614923073804552E-4 6.7568024108271E-5 3.37840120541355E-5 3.4171007912215625 0.0018581206629774524 88-89 8.78384313407523E-4 6.7568024108271E-5 3.37840120541355E-5 3.6633017790660745 0.0019256886870857237 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7485 0.0 51.26966 1 GTATCAA 13120 0.0 41.54593 1 TATCAAC 16795 0.0 32.31565 2 ATCAACG 16970 0.0 31.926928 3 TCAACGC 17015 0.0 31.842491 4 CAACGCA 17240 0.0 31.261507 5 AACGCAG 17900 0.0 30.238535 6 ACGCAGA 20020 0.0 27.02503 7 CGCAGAG 20060 0.0 26.876421 8 TGGTATC 3640 0.0 25.982351 2 GCAGAGT 21610 0.0 24.486662 9 GTGGTAT 3915 0.0 24.38341 1 TAAGGTG 2045 0.0 24.169859 5 AGGTAAG 1755 0.0 23.559654 2 GGTAAGG 1945 0.0 23.457718 3 GAGTACT 13510 0.0 23.293032 12-13 GTAAGGT 1995 0.0 22.869804 4 GTACTTT 14015 0.0 22.031925 14-15 AGAGTAC 19035 0.0 21.70955 10-11 GTACATG 11120 0.0 21.633259 1 >>END_MODULE