FastQCFastQC Report
Thu 26 May 2016
SRR938290_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938290_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2959980
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT149440.5048682761370009No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT122780.4148001000006757No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79220.26763694349286143No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC42040.14202798667558567No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA37120.12540625274495099No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA35160.11878458638234042No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA34270.11577780930952235No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA33280.11243319211616296No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32040.10824397462145013No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA31070.10496692545219902No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA71850.050.0471461
GTATCAA127250.042.2570151
TATCAAC163650.032.68382
ATCAACG163950.032.6502153
TCAACGC166350.032.2917334
CAACGCA169750.031.5889855
AACGCAG174150.030.7908736
ACGCAGA198800.026.7340777
CGCAGAG200150.026.458848
GCAGAGT215000.024.4325149
TGGTATC35750.024.1827412
TAAGGTG20800.023.7476965
GTAAGGT19800.023.028074
GGTAAGG18950.022.8089713
GTGGTAT39600.022.7912771
GAGTACT135500.022.67861612-13
AGTACTT139300.021.94062212-13
TACATGG104600.021.9343952
GTACATG108600.021.8263421
AGAGTAC188850.021.7671510-11
Produced by FastQC (version 0.11.5)