FastQCFastQC Report
Thu 26 May 2016
SRR938289_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938289_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2968039
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT158860.5352355545193308No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT123670.4166724224311069No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81590.2748953096640576No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC38420.12944573841516235No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA36740.12378543543396835No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA34980.11785559421557466No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA34630.11667636442782592No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA32810.11054436953153245No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA31170.10501883566893831No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30610.10313206800854031No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA74100.049.4156341
GTATCAA128550.041.222861
TATCAAC166300.031.8609072
ATCAACG166300.031.6608833
TCAACGC167650.031.4059374
CAACGCA170400.030.8712065
AACGCAG175000.030.0597326
ACGCAGA199500.026.1666097
CGCAGAG201000.025.8521988
TAAGGTG20200.024.700945
GTGTAAG14950.024.5887681
AGGTAAG19200.023.5124532
TAAGGTA10950.023.4344734
GCAGAGT218250.023.3063139
GGTAAGG20200.023.0542093
GAGTACT131200.022.7523312-13
CGCGCGT2303.594323E-922.7380337
GTAAGGT20550.022.6615584
TAGCGCG2354.634785E-922.243364
GTACTTT136350.021.70492614-15
Produced by FastQC (version 0.11.5)