##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938289_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2968039 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.81009413959857 33.0 31.0 34.0 30.0 34.0 2 32.007261360110164 34.0 31.0 34.0 30.0 34.0 3 32.07534267575325 34.0 31.0 34.0 30.0 34.0 4 35.60830299062782 37.0 35.0 37.0 33.0 37.0 5 35.623547736401036 37.0 35.0 37.0 33.0 37.0 6 35.653805088140686 37.0 35.0 37.0 33.0 37.0 7 35.590732466790364 37.0 35.0 37.0 33.0 37.0 8 35.56696054196053 37.0 35.0 37.0 33.0 37.0 9 37.21631690149624 39.0 37.0 39.0 34.0 39.0 10-11 37.13380113940552 39.0 37.0 39.0 34.0 39.0 12-13 37.03471130264798 39.0 37.0 39.0 33.0 39.0 14-15 38.25876193001507 40.0 38.0 41.0 33.0 41.0 16-17 38.17548876547781 40.0 38.0 41.0 33.0 41.0 18-19 38.12243976578475 40.0 38.0 41.0 33.0 41.0 20-21 38.010987389316654 40.0 38.0 41.0 33.0 41.0 22-23 37.853335653608326 40.0 38.0 41.0 33.0 41.0 24-25 37.79409771906636 40.0 38.0 41.0 32.5 41.0 26-27 37.794193742063364 40.0 38.0 41.0 33.0 41.0 28-29 37.69585305314385 40.0 38.0 41.0 32.0 41.0 30-31 37.482964678024786 40.0 37.0 41.0 32.0 41.0 32-33 37.23577773068346 40.0 37.0 41.0 31.5 41.0 34-35 37.00813516938288 40.0 36.0 41.0 31.0 41.0 36-37 36.765177950828814 40.0 36.0 41.0 30.0 41.0 38-39 36.4932239771782 39.0 35.5 41.0 30.0 41.0 40-41 36.24283390481055 39.0 35.0 41.0 30.0 41.0 42-43 35.927398191196275 39.0 35.0 40.5 28.5 41.0 44-45 35.65467367511006 39.0 35.0 40.0 27.0 41.0 46-47 35.46904808191536 39.0 35.0 41.0 26.5 41.0 48-49 35.16636422230301 39.0 35.0 40.5 25.0 41.0 50-51 34.502044784451954 38.5 34.0 39.5 24.0 40.5 52-53 34.57189258631709 38.0 34.0 40.0 24.0 41.0 54-55 34.686395630246096 38.0 34.5 40.0 24.0 41.0 56-57 34.44782767342343 38.0 34.0 40.0 23.0 41.0 58-59 34.15877638400304 38.0 34.0 40.0 22.5 41.0 60-61 33.760301498733675 37.5 34.0 40.0 21.5 41.0 62-63 33.316933503906114 37.0 33.0 40.0 19.5 41.0 64-65 32.85989217122821 36.0 33.0 39.0 18.0 41.0 66-67 32.41088122494347 36.0 32.0 39.0 14.0 41.0 68-69 31.90532300956962 35.0 31.5 39.0 9.5 40.0 70-71 31.40273072557335 35.0 31.0 38.0 7.0 40.0 72-73 30.90439967264581 35.0 31.0 37.0 4.0 39.5 74-75 30.37288357733844 35.0 30.0 36.5 2.0 39.0 76-77 29.83325775031932 34.0 29.5 36.0 2.0 39.0 78-79 29.26214143412536 34.0 29.0 35.5 2.0 37.5 80-81 28.73256348720485 34.0 28.5 35.0 2.0 37.0 82-83 28.187109906574676 34.0 27.0 35.0 2.0 36.5 84-85 27.686364127964627 33.5 26.0 35.0 2.0 36.0 86-87 27.17457486239231 33.0 25.5 35.0 2.0 36.0 88-89 26.599728305456903 33.0 25.0 35.0 2.0 35.0 90-91 25.964680214781545 32.0 23.5 35.0 2.0 35.0 92-93 25.322296472519398 32.0 20.0 34.0 2.0 35.0 94-95 24.67092480927643 32.0 16.5 34.0 2.0 35.0 96-97 23.783735658460014 31.0 4.5 34.0 2.0 35.0 98-99 22.653703674378942 31.0 2.0 34.0 2.0 35.0 100-101 19.767054947728113 27.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.5320803202683244 1101 2 0.36403813278524666 1101 3 0.33988583222718916 1101 4 0.27220775855592194 1101 5 0.2804017211126819 1101 6 0.36719461241324325 1101 7 0.4207109304236454 1101 8 0.3137751646173541 1101 9 0.36413952902265123 1101 10-11 0.44590428885321387 1101 12-13 0.30111640820624075 1101 14-15 0.3910017652111719 1101 16-17 0.5178241809156603 1101 18-19 0.4816603225499776 1101 20-21 0.5754726475628757 1101 22-23 0.5874425546526467 1101 24-25 0.5390940947419054 1101 26-27 0.4934141239987824 1101 28-29 0.578057152995207 1101 30-31 0.6886517359876976 1101 32-33 0.7020365943592353 1101 34-35 0.7013951510547258 1101 36-37 0.7195238246224491 1101 38-39 0.6868077546353817 1101 40-41 0.7311111132792263 1101 42-43 0.6525229418481047 1101 44-45 0.7959412532301542 1101 46-47 0.8429524720254733 1101 48-49 0.7583672924567821 1101 50-51 0.7602240884634668 1101 52-53 0.730279671359078 1101 54-55 0.614760574705123 1101 56-57 0.7398560978934512 1101 58-59 0.6159064593834884 1101 60-61 0.5897493917460466 1101 62-63 0.5523349421155785 1101 64-65 0.573695253723038 1101 66-67 0.5607478969221376 1101 68-69 0.5986806760841894 1101 70-71 0.7757980817177916 1101 72-73 0.8036488599610436 1101 74-75 0.7576762885281667 1101 76-77 0.7551964600606347 1101 78-79 0.7605176919118897 1101 80-81 0.8444155410796945 1101 82-83 0.8054648191276748 1101 84-85 0.8695709781260206 1101 86-87 1.082005586253647 1101 88-89 1.0715148067941733 1101 90-91 1.2140485579478586 1101 92-93 1.3777591067518422 1101 94-95 1.3124097371229162 1101 96-97 1.2441304827105775 1101 98-99 1.3967078716226524 1101 100-101 1.377159114356445 1102 1 0.3678960146067318 1102 2 0.3790159456769757 1102 3 0.21792811734120932 1102 4 0.29492955648461816 1102 5 0.30069229198329595 1102 6 0.26586220362896285 1102 7 0.15204711366170187 1102 8 0.2728721450247775 1102 9 0.3265749945824581 1102 10-11 0.3708877589920263 1102 12-13 0.20566440332694214 1102 14-15 0.2624860477294817 1102 16-17 0.3381708217848072 1102 18-19 0.29804851610585814 1102 20-21 0.26330943332435197 1102 22-23 0.44116754446280027 1102 24-25 0.3991917635363933 1102 26-27 0.3631084966931297 1102 28-29 0.3788469064615967 1102 30-31 0.36606288632120254 1102 32-33 0.4974083662332518 1102 34-35 0.49895415534377463 1102 36-37 0.421290694613063 1102 38-39 0.25803837730794044 1102 40-41 0.2607014121349778 1102 42-43 0.36995379500766745 1102 44-45 0.22271832784226575 1102 46-47 0.3254337009899899 1102 48-49 0.3488687627794391 1102 50-51 0.5433307328912989 1102 52-53 0.4767789172072483 1102 54-55 0.5466768326696823 1102 56-57 0.4115182260528556 1102 58-59 0.4817524853152051 1102 60-61 0.42703622156251697 1102 62-63 0.4106286614185848 1102 64-65 0.3777575797974251 1102 66-67 0.3922908031806003 1102 68-69 0.4103714924568642 1102 70-71 0.5231293625995157 1102 72-73 0.6345221479713743 1102 74-75 0.4677445183566391 1102 76-77 0.45518620371403884 1102 78-79 0.4759640750127403 1102 80-81 0.6362541936735902 1102 82-83 0.7064991288238254 1102 84-85 0.745842048341796 1102 86-87 0.6390857758098463 1102 88-89 0.8026220695585238 1102 90-91 1.0585257665427363 1102 92-93 1.0574627131138499 1102 94-95 1.0569533311773078 1102 96-97 1.1321828290937113 1102 98-99 1.417928164055624 1102 100-101 1.1469916964936715 1103 1 0.3441865045491035 1103 2 0.29644500883982516 1103 3 0.3114288903641622 1103 4 0.16230965458143487 1103 5 0.19788107210722927 1103 6 0.20781255513562513 1103 7 0.1421807570376643 1103 8 0.11871636527173024 1103 9 0.27727238213991257 1103 10-11 0.29740432168606645 1103 12-13 0.14975213205631377 1103 14-15 0.37337066936495944 1103 16-17 0.38129821013404097 1103 18-19 0.3236495362461298 1103 20-21 0.40201600547802485 1103 22-23 0.3658088493088911 1103 24-25 0.4044101894032792 1103 26-27 0.3098795110409469 1103 28-29 0.4900003150552834 1103 30-31 0.49571279651719635 1103 32-33 0.4715889827346942 1103 34-35 0.5232299932714852 1103 36-37 0.4544421279351383 1103 38-39 0.4630444823140394 1103 40-41 0.5777827191480327 1103 42-43 0.3699990095580006 1103 44-45 0.5310180107153286 1103 46-47 0.6776075415597376 1103 48-49 0.6339850496022095 1103 50-51 0.530835264764427 1103 52-53 0.5953239671516215 1103 54-55 0.5359851542305591 1103 56-57 0.5621791502632618 1103 58-59 0.6442519123197528 1103 60-61 0.5600649296765496 1103 62-63 0.6848457412670612 1103 64-65 0.7219319871117591 1103 66-67 0.692795039179714 1103 68-69 0.6779501595235864 1103 70-71 0.8656106204391385 1103 72-73 0.8668749298122123 1103 74-75 0.7450997380326783 1103 76-77 0.7698880168800137 1103 78-79 0.7700784740759445 1103 80-81 0.7274696524658388 1103 82-83 0.8257867808759833 1103 84-85 0.9968200994904954 1103 86-87 0.8662255491953559 1103 88-89 0.6965754048087867 1103 90-91 0.8774075294518013 1103 92-93 0.9427725682189241 1103 94-95 1.0377194365167952 1103 96-97 0.9064749192561088 1103 98-99 0.9072021058892936 1103 100-101 0.8140288307663042 1104 1 0.35002664862044597 1104 2 0.3680296915665693 1104 3 0.1933355398891976 1104 4 0.21921783460939537 1104 5 0.24340001463174588 1104 6 0.17933244657195502 1104 7 0.22735426505239786 1104 8 0.27330404778420103 1104 9 0.15687536968162163 1104 10-11 0.2731876840989571 1104 12-13 0.15778829273870798 1104 14-15 0.2691616587840784 1104 16-17 0.3645485136837934 1104 18-19 0.39104816593584957 1104 20-21 0.3274275832224731 1104 22-23 0.4187397624069291 1104 24-25 0.4208726614716056 1104 26-27 0.4292493009539129 1104 28-29 0.4913475402534786 1104 30-31 0.45308178546696354 1104 32-33 0.4280702799889653 1104 34-35 0.475397695578458 1104 36-37 0.5311343540006277 1104 38-39 0.5666115744605236 1104 40-41 0.682703286046312 1104 42-43 0.6370076160861089 1104 44-45 0.5437065176587126 1104 46-47 0.6732847635948929 1104 48-49 0.6804568577383634 1104 50-51 0.6565935014209927 1104 52-53 0.7068144729318178 1104 54-55 0.7374518861986772 1104 56-57 0.7484870160279513 1104 58-59 0.8574994354558569 1104 60-61 0.8529151852705397 1104 62-63 0.704979634764463 1104 64-65 0.721609943788323 1104 66-67 0.753138560560842 1104 68-69 0.8489463638763333 1104 70-71 0.9137205768708192 1104 72-73 0.8646282790627744 1104 74-75 0.6260836218368979 1104 76-77 0.6910787226459298 1104 78-79 0.7112019397467613 1104 80-81 0.8724081517567086 1104 82-83 0.8086103291939573 1104 84-85 0.6666962307872417 1104 86-87 0.7176119297600074 1104 88-89 0.7451830297743491 1104 90-91 0.869599668788787 1104 92-93 0.9521823552749531 1104 94-95 1.0740108493750604 1104 96-97 0.9672462162042841 1104 98-99 0.8582556020681409 1104 100-101 0.6162786700634939 1105 1 0.4531680251253185 1105 2 0.3469470059926181 1105 3 0.3593918194219583 1105 4 0.3097920712920086 1105 5 0.281449741905071 1105 6 0.2533927258433053 1105 7 0.2688083400406285 1105 8 0.29633368165136886 1105 9 0.33111143665900755 1105 10-11 0.36781706659728286 1105 12-13 0.2633281627353625 1105 14-15 0.3576894391138481 1105 16-17 0.41036951851511105 1105 18-19 0.5232619378986456 1105 20-21 0.4927404233020667 1105 22-23 0.49554269927395467 1105 24-25 0.47340350482638627 1105 26-27 0.45083266420083135 1105 28-29 0.5665872948894588 1105 30-31 0.565964000600431 1105 32-33 0.5503911394355185 1105 34-35 0.5004678070274835 1105 36-37 0.4470080738332882 1105 38-39 0.5304136087107096 1105 40-41 0.5418950603939976 1105 42-43 0.5143163096216483 1105 44-45 0.4613452772377613 1105 46-47 0.48752878884921813 1105 48-49 0.4396970186939839 1105 50-51 0.42683448259295886 1105 52-53 0.4921836981494039 1105 54-55 0.5433452258714837 1105 56-57 0.5502630543451303 1105 58-59 0.5959098542871075 1105 60-61 0.5120547963213014 1105 62-63 0.6373481917803971 1105 64-65 0.615372333459284 1105 66-67 0.6808875994586145 1105 68-69 0.63464744048132 1105 70-71 0.7830257679120898 1105 72-73 0.8094762477960522 1105 74-75 0.7931591930285506 1105 76-77 0.9150101564428219 1105 78-79 0.8413230234741853 1105 80-81 0.8613277132535195 1105 82-83 0.9605682523796553 1105 84-85 0.835502153014442 1105 86-87 0.9302304041386691 1105 88-89 0.9634562967831819 1105 90-91 0.9781122310745616 1105 92-93 1.0008963157170356 1105 94-95 1.10288316319021 1105 96-97 1.202069786816292 1105 98-99 1.1358510043103784 1105 100-101 1.1640510628694614 1106 1 0.05308687159373093 1106 2 0.12532716630308727 1106 3 0.03924067672657827 1106 4 0.09161331175505438 1106 5 0.024733878404667564 1106 6 0.06239763593134029 1106 7 0.11085878856703602 1106 8 0.05502512931499837 1106 9 0.14800544183335518 1106 10-11 -0.3593417203969196 1106 12-13 -0.15042289327957548 1106 14-15 -0.008783754831462431 1106 16-17 0.17102399963972204 1106 18-19 0.13293481017752384 1106 20-21 0.01086663109126107 1106 22-23 -0.05768523195695252 1106 24-25 -0.024484062298284925 1106 26-27 0.1396303449231695 1106 28-29 -0.03637573016600726 1106 30-31 0.16436490296138118 1106 32-33 0.11088558183487862 1106 34-35 -0.05030582862193711 1106 36-37 -0.08631018330481055 1106 38-39 0.10233984097953197 1106 40-41 0.2317667473108571 1106 42-43 0.1715406838223288 1106 44-45 0.2966834605155597 1106 46-47 0.34874634744286936 1106 48-49 0.3983632382946567 1106 50-51 0.3118817947103807 1106 52-53 0.41076055675008405 1106 54-55 0.004079375169347088 1106 56-57 0.1310622876699341 1106 58-59 0.3057459662960227 1106 60-61 0.023858501495858775 1106 62-63 0.2581177847219678 1106 64-65 0.28530331495989003 1106 66-67 -0.030754489517121897 1106 68-69 -0.30471954124635303 1106 70-71 -0.08767200392374619 1106 72-73 0.18315538713567747 1106 74-75 0.38477367736797774 1106 76-77 0.2979666827676901 1106 78-79 0.5001862430425028 1106 80-81 0.5127685165997882 1106 82-83 0.5449068413099596 1106 84-85 0.4646963760528564 1106 86-87 0.5345099287733319 1106 88-89 0.28662835896603767 1106 90-91 0.24375354374905456 1106 92-93 0.3100311083017111 1106 94-95 0.3368484032719046 1106 96-97 0.5621112064365867 1106 98-99 0.37623316513369787 1106 100-101 -0.0918374330071714 1107 1 0.39493587380380646 1107 2 0.3192723594251845 1107 3 0.30291202170494813 1107 4 0.245080501765095 1107 5 0.20848814000967053 1107 6 0.20175692630419917 1107 7 0.1600044517296837 1107 8 0.1988565098246866 1107 9 0.32693801899416286 1107 10-11 0.270357110297212 1107 12-13 0.2731677437281448 1107 14-15 0.2840266207682376 1107 16-17 0.32962171343975655 1107 18-19 0.33134990230948347 1107 20-21 0.36077136636861695 1107 22-23 0.46043237524914105 1107 24-25 0.47193963504660985 1107 26-27 0.4987117938234036 1107 28-29 0.577142887785044 1107 30-31 0.552367529684382 1107 32-33 0.5561339751088639 1107 34-35 0.5459444081567213 1107 36-37 0.5438666417368765 1107 38-39 0.49174555585497615 1107 40-41 0.5666083063721885 1107 42-43 0.5939166695966591 1107 44-45 0.5603975872479268 1107 46-47 0.5448542924363835 1107 48-49 0.5980654531843967 1107 50-51 0.5754300531314627 1107 52-53 0.5127978337358101 1107 54-55 0.46880601071689654 1107 56-57 0.47553050827654886 1107 58-59 0.43935064514089817 1107 60-61 0.49311408692499725 1107 62-63 0.6348110191682892 1107 64-65 0.5456788215347572 1107 66-67 0.5176754968003934 1107 68-69 0.6088034699142604 1107 70-71 0.721433563806567 1107 72-73 0.8142259180884679 1107 74-75 0.7227029177710875 1107 76-77 0.8786571244035706 1107 78-79 0.7386179417257317 1107 80-81 0.7866135927485693 1107 82-83 0.8092897547704148 1107 84-85 0.8539854134173765 1107 86-87 0.8702879754997745 1107 88-89 1.0553646582599612 1107 90-91 1.2496214365411547 1107 92-93 1.2012159634950628 1107 94-95 1.1485777359871925 1107 96-97 1.2541658404345704 1107 98-99 1.3462542755464462 1107 100-101 1.1798278004798632 1108 1 0.31960526055038585 1108 2 0.29381689261947486 1108 3 0.14705249006543397 1108 4 0.13468420559676275 1108 5 0.16183782732612428 1108 6 0.2625549001692349 1108 7 0.17125362591635707 1108 8 0.1952269097434396 1108 9 0.24426978603052163 1108 10-11 0.2596540460717165 1108 12-13 0.15984317370074308 1108 14-15 0.2536277908842095 1108 16-17 0.3288212372687198 1108 18-19 0.2884651413529937 1108 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1.0877999295076002 1108 88-89 1.1305204241906992 1108 90-91 1.048092831205068 1108 92-93 1.1426676569489658 1108 94-95 1.310677223066815 1108 96-97 1.2050132741051414 1108 98-99 1.2737940899570077 1108 100-101 0.9466651265093944 1109 1 0.29545188859628624 1109 2 0.27208016306856564 1109 3 0.2001436571554578 1109 4 0.21899795077335682 1109 5 0.2342039012945918 1109 6 0.21571433966616382 1109 7 0.28526821077661424 1109 8 0.2364510607727084 1109 9 0.22886164086464333 1109 10-11 0.33610374704526436 1109 12-13 0.19789884917923928 1109 14-15 0.24381530573513288 1109 16-17 0.24018374032257128 1109 18-19 0.3326793895365796 1109 20-21 0.2011436272530105 1109 22-23 0.3165033805618478 1109 24-25 0.23160410889296656 1109 26-27 0.24318064630691794 1109 28-29 0.24526110046084426 1109 30-31 0.24920212001686082 1109 32-33 0.1177356316734759 1109 34-35 0.21451416583797567 1109 36-37 0.10906577040780263 1109 38-39 0.10573457402171726 1109 40-41 0.19742638124257184 1109 42-43 0.20530381161565003 1109 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-1.8693141832472868 2316 100-101 -1.8861403703927238 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8800.0 3 2591.0 4 2632.0 5 2874.0 6 3433.0 7 4246.0 8 5215.0 9 6617.0 10 8278.0 11 9916.0 12 12696.0 13 15484.0 14 18101.0 15 22026.0 16 27016.0 17 35425.0 18 31443.0 19 25505.0 20 24335.0 21 25170.0 22 26892.0 23 29092.0 24 31961.0 25 35449.0 26 39870.0 27 46165.0 28 53626.0 29 63637.0 30 75506.0 31 92567.0 32 115221.0 33 150131.0 34 206060.0 35 300340.0 36 439475.0 37 559782.0 38 371073.0 39 39386.0 40 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.466359665458974 18.58856194765178 15.156265872075306 22.788812514813937 2 15.53207554801978 19.73066719701745 40.17066733185239 24.56658992311037 3 19.290510256953965 23.897888602376877 29.559802705633796 27.251798435035358 4 13.028570619425109 16.736315059999885 36.58834916956302 33.646765151011984 5 14.044953860232939 36.96576448504095 33.71633931434556 15.272942340380549 6 32.32498212382456 35.894786902497266 16.9262084969172 14.854022476760973 7 28.76416738144174 32.86822379742465 20.04343944371618 18.324169377417423 8 26.847146168440045 34.07004558956982 19.361746022542633 19.721062219447504 9 26.47709716903123 16.89388645449149 20.099976032450243 36.52904034402704 10-11 25.160419756741238 26.43772550730609 26.502109202434543 21.89974553351813 12-13 26.455348840189973 23.768170885501707 27.00709886776314 22.76938140654518 14-15 23.11259454683425 25.057334056547575 25.747753074699965 26.082318321918212 16-17 22.18723224789309 28.24249434428325 26.653449064330964 22.916824343492696 18-19 22.30244628322622 26.33838293172701 29.38521725308388 21.973953531962895 20-21 22.765585800739863 25.470186709123777 29.89014998062085 21.87407750951551 22-23 22.586750097348908 25.31975112393738 29.807240735839734 22.28625804287398 24-25 22.397544476767273 25.61858085419606 29.788293066374095 22.19558160266258 26-27 21.85549124080787 25.983482102745548 29.605278795356327 22.555747861090257 28-29 22.077454229086275 26.39855686783274 29.421383365781107 22.10260553729988 30-31 22.122928271331087 25.87741967894843 29.363817419159723 22.635834630560755 32-33 21.82873833296077 25.90262247293523 29.430929340806173 22.837709853297824 34-35 22.71376529459851 25.98585139531312 29.089016042649124 22.211367267439243 36-37 22.806609117121102 25.48735276585759 29.107232443162967 22.598805673858337 38-39 22.239575091212103 25.991333901950163 29.022614400431973 22.746476606405754 40-41 22.762151785879812 25.795391919426848 29.125556797095808 22.316899497597536 42-43 22.434838707937732 25.855604302559804 28.81263667961893 22.896920309883537 44-45 22.40939384600505 26.160182207290276 29.002107744871374 22.428316201833294 46-47 22.93755163756483 26.43487764793388 28.1729498443901 22.45462087011119 48-49 22.63923517798417 26.518128023499806 28.037098599542865 22.805538198973164 50-51 22.782403264970487 26.68338245280571 28.061575429142422 22.472638853081385 52-53 23.10348331322791 26.43320446652156 27.96054832117881 22.502763899071724 54-55 22.644580333266493 26.67025017735008 28.07024579625551 22.614923693127917 56-57 22.674520490330945 26.760319643905472 27.894312746056855 22.670847119706725 58-59 22.648243608176774 26.363072509999903 27.881891397010417 23.106792484812907 60-61 22.337385194641676 26.65821536561791 28.180848181245864 22.82355125849455 62-63 22.42532110190676 26.622173740445458 28.389118829104433 22.56338632854334 64-65 22.139382073940098 26.684143472239562 28.193514676253635 22.982959777566702 66-67 22.223023479747233 26.63257577991328 28.06631379509917 23.07808694524032 68-69 22.645697258380167 26.352490608669427 28.24374746735254 22.758064665597864 70-71 22.599611874974194 26.542291387011886 27.693689639859414 23.16440709815451 72-73 22.591598115433015 26.63175192365606 27.696535304117052 23.080114656793874 74-75 22.425364526257837 27.108987903863852 27.572756537560437 22.892891032317873 76-77 22.738267287619216 26.665102995861833 27.63417449723687 22.96245521928208 78-79 22.7597288758599 26.896648285188167 27.601922178428453 22.74170066052348 80-81 22.98094826817832 26.935387749827257 27.24357381259234 22.84009016940208 82-83 22.94903496437511 26.977710116087355 27.291438315482274 22.781816604055262 84-85 22.826378521915828 27.16386575860407 27.30071541942854 22.70904030005156 86-87 23.087122360821894 27.099515462736456 26.97666269063071 22.836699485810943 88-89 22.935615151057565 27.189788648841045 26.83230102574612 23.042295174355267 90-91 22.98325515182988 27.140670579365644 26.890577413846543 22.985496854957933 92-93 23.04403883788333 26.779885687862176 27.113921008785997 23.062154465468495 94-95 22.98521686410494 26.509862194077137 27.286091864884494 23.218829076933424 96-97 23.23353854518287 26.25187008841092 27.23467243769869 23.27991892870752 98-99 23.00191232943565 26.5214377050429 26.905790981111956 23.570858984409494 100-101 23.553570343770883 25.828850294631827 27.114970536817168 23.502608824780122 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1301.0 1 888.5 2 669.5 3 801.5 4 621.5 5 616.0 6 1205.0 7 2958.5 8 5087.0 9 5841.0 10 4746.0 11 3542.0 12 3412.0 13 3558.0 14 3711.5 15 3923.0 16 4228.5 17 4513.5 18 4881.5 19 5415.5 20 5989.0 21 6961.5 22 8353.5 23 10116.0 24 12398.5 25 15223.5 26 19098.0 27 23367.5 28 26513.0 29 29896.5 30 33864.5 31 39000.5 32 47074.5 33 50744.0 34 50453.0 35 53083.0 36 59210.0 37 65677.5 38 69989.5 39 73273.0 40 77192.5 41 81963.0 42 84457.0 43 87353.0 44 91874.5 45 97741.5 46 101275.0 47 104358.5 48 107602.0 49 106622.0 50 104759.0 51 102812.0 52 102321.5 53 105039.5 54 109255.0 55 106101.0 56 97806.5 57 89670.0 58 83975.5 59 76085.5 60 61923.5 61 51716.5 62 44101.5 63 33076.5 64 23202.5 65 17730.5 66 13488.5 67 10385.5 68 8173.5 69 6500.0 70 5077.0 71 3496.0 72 2517.5 73 2026.5 74 1491.5 75 1018.0 76 749.5 77 531.0 78 362.0 79 220.5 80 128.0 81 79.0 82 64.0 83 42.5 84 22.5 85 16.5 86 16.0 87 11.0 88 6.5 89 7.0 90 7.5 91 7.0 92 7.5 93 5.5 94 2.0 95 1.5 96 2.5 97 1.5 98 1.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.49659050976082186 2 0.04882011321279808 3 0.05761379820143872 4 0.06569994531743013 5 0.08005285644831486 6 0.10778160260023538 7 0.06977671115507579 8 0.052930571330093705 9 0.051481803305145256 10-11 0.07499901450082024 12-13 0.10781529487988535 14-15 0.0556596459817408 16-17 0.06039341127256077 18-19 0.05941633516271182 20-21 0.07488109152204536 22-23 0.06415010045353178 24-25 0.08903184897503032 26-27 0.06682863668570392 28-29 0.06706448264325368 30-31 0.07068640270562483 32-33 0.07068640270562483 34-35 0.07289324702269748 36-37 0.06788994349467779 38-39 0.04011065892328234 40-41 0.04359780986705363 42-43 0.042014272723505316 44-45 0.021883135632651728 46-47 0.028419437884744776 48-49 0.029143821897219008 50-51 0.02641474724557191 52-53 0.025724055512747642 54-55 0.02506705605957334 56-57 0.02483121010202359 58-59 0.021377751437902263 60-61 0.02840259174491979 62-63 0.020249060069628465 64-65 0.022826519462850726 66-67 0.01622283265145775 68-69 0.018244369430455598 70-71 0.04081819679593159 72-73 0.036977276915835675 74-75 0.03148543533289152 76-77 0.022321135268101262 78-79 0.01578483301600821 80-81 0.017300985600256603 82-83 0.022287442988451298 84-85 0.018497061527830327 86-87 0.008726300429340719 88-89 0.04120565801190618 90-91 0.05203772591936966 92-93 0.033271126154339616 94-95 0.053385417105368226 96-97 0.04270496445632958 98-99 0.03291735721801499 100-101 0.16910155156317017 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2968039.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.219637682026686 #Duplication Level Percentage of deduplicated Percentage of total 1 73.23752467567233 35.31486904589409 2 14.070325685311413 13.569320132276601 3 5.12422415443092 7.412646963844456 4 2.394311112978213 4.618112574634379 5 1.332417820654571 3.2124352276519517 6 0.8423817833277272 2.4371606629201508 7 0.5283794627243404 1.7834786375834137 8 0.37409007489665785 1.4430790297567255 9 0.3000149054932319 1.301994903788201 >10 1.6102286112516178 14.259894881672386 >50 0.12117475062027863 4.03999085285229 >100 0.05813187777777333 5.204062244403762 >500 0.0028020968498701955 0.9403066830841161 >1k 0.0037828307472275117 3.2326863482433423 >5k 7.005242124495392E-5 0.27560305446460776 >10k+ 1.4010484248990783E-4 0.9543587569295946 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15886 0.5352355545193308 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12367 0.4166724224311069 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8159 0.2748953096640576 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 3842 0.12944573841516235 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 3674 0.12378543543396835 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 3498 0.11785559421557466 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 3463 0.11667636442782592 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 3281 0.11054436953153245 No Hit GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 3117 0.10501883566893831 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3061 0.10313206800854031 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 6.738455929992834E-5 2 0.0 0.0 0.0 0.0 6.738455929992834E-5 3 0.0 0.0 0.0 0.0 6.738455929992834E-5 4 0.0 0.0 0.0 0.0 6.738455929992834E-5 5 0.0 0.0 0.0 0.0 1.010768389498925E-4 6 0.0 0.0 0.0 0.0 1.010768389498925E-4 7 3.369227964996417E-5 0.0 0.0 0.0 1.010768389498925E-4 8 3.369227964996417E-5 0.0 0.0 0.0 1.010768389498925E-4 9 3.369227964996417E-5 0.0 0.0 0.0 1.010768389498925E-4 10-11 3.369227964996417E-5 0.0 0.0 3.369227964996417E-5 1.010768389498925E-4 12-13 3.369227964996417E-5 0.0 0.0 3.369227964996417E-5 3.200766566746596E-4 14-15 3.369227964996417E-5 0.0 0.0 8.423069912491041E-5 3.874612159745879E-4 16-17 3.369227964996417E-5 0.0 0.0 1.010768389498925E-4 4.7169191509949834E-4 18-19 5.053841947494626E-5 0.0 0.0 1.010768389498925E-4 5.053841947494625E-4 20-21 6.738455929992834E-5 0.0 0.0 1.1792297877487459E-4 5.559226142244088E-4 22-23 6.738455929992834E-5 0.0 0.0 1.6846139824982083E-4 5.727687540493908E-4 24-25 6.738455929992834E-5 0.0 0.0 3.874612159745879E-4 5.727687540493908E-4 26-27 1.1792297877487459E-4 0.0 0.0 8.423069912491042E-4 5.727687540493908E-4 28-29 1.6846139824982083E-4 0.0 0.0 0.0025943055330472408 5.727687540493908E-4 30-31 2.02153677899785E-4 0.0 0.0 0.00938329988251502 6.06461033699355E-4 32-33 2.02153677899785E-4 0.0 0.0 0.024275287487799183 6.738455929992833E-4 34-35 2.1899981772476707E-4 0.0 0.0 0.04615842312045091 6.738455929992833E-4 36-37 2.3584595754974917E-4 0.0 0.0 0.08027185626603964 6.738455929992833E-4 38-39 2.6953823719971337E-4 3.369227964996417E-5 0.0 0.13166942887205996 7.243840124742296E-4 40-41 2.863843770246954E-4 3.369227964996417E-5 0.0 0.18379138549055452 7.412301522992117E-4 42-43 3.032305168496775E-4 3.369227964996417E-5 0.0 0.23867610904034617 7.580762921241939E-4 44-45 3.3692279649964166E-4 3.369227964996417E-5 0.0 0.30599328378097457 8.086147115991401E-4 46-47 3.874612159745879E-4 3.369227964996417E-5 0.0 0.3870063701993134 8.759992708990684E-4 48-49 4.0430735579957E-4 3.369227964996417E-5 0.0 0.46906391728680114 9.602299700239787E-4 50-51 4.0430735579957E-4 3.369227964996417E-5 0.0 0.5600667646213544 9.770761098489608E-4 52-53 4.0430735579957E-4 3.369227964996417E-5 0.0 0.6476835378510862 0.0010949990886238354 54-55 4.0430735579957E-4 3.369227964996417E-5 0.0 0.7414491521169364 0.0011623836479237638 56-57 4.0430735579957E-4 3.369227964996417E-5 0.0 0.8474450638957238 0.001196075927573728 58-59 4.0430735579957E-4 3.369227964996417E-5 0.0 0.9640203514845997 0.00121292206739871 60-61 4.0430735579957E-4 3.369227964996417E-5 0.0 1.088782863028417 0.00121292206739871 62-63 4.0430735579957E-4 3.369227964996417E-5 0.0 1.2089632245398392 0.0012803066266986384 64-65 4.0430735579957E-4 3.369227964996417E-5 0.0 1.3359325804007292 0.0012803066266986384 66-67 4.0430735579957E-4 3.369227964996417E-5 0.0 1.4777433854474284 0.0012803066266986384 68-69 4.0430735579957E-4 3.369227964996417E-5 0.0 1.638186021140558 0.0012971527665236204 70-71 4.3799963544953414E-4 3.369227964996417E-5 0.0 1.8042384213954061 0.001381383465648531 72-73 5.053841947494625E-4 3.369227964996417E-5 0.0 1.9751425099198494 0.0015329987240733697 74-75 5.390764743994267E-4 3.369227964996417E-5 0.0 2.150325517959838 0.0016677678426732265 76-77 5.390764743994267E-4 6.738455929992834E-5 0.0 2.33204482825192 0.0017183062621481725 78-79 5.559226142244088E-4 6.738455929992834E-5 0.0 2.5374498111379262 0.0017519985417981368 80-81 6.738455929992833E-4 6.738455929992834E-5 0.0 2.756163244485669 0.0018362292409230471 82-83 6.906917328242655E-4 1.010768389498925E-4 0.0 2.987848205498647 0.0019541522196979215 84-85 7.412301522992117E-4 1.010768389498925E-4 0.0 3.2200553968462007 0.0019541522196979215 86-87 8.0861471159914E-4 1.010768389498925E-4 0.0 3.464038039931416 0.001987844499347886 88-89 8.0861471159914E-4 1.010768389498925E-4 0.0 3.7175050597380963 0.00202153677899785 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7410 0.0 49.415634 1 GTATCAA 12855 0.0 41.22286 1 TATCAAC 16630 0.0 31.860907 2 ATCAACG 16630 0.0 31.660883 3 TCAACGC 16765 0.0 31.405937 4 CAACGCA 17040 0.0 30.871206 5 AACGCAG 17500 0.0 30.059732 6 ACGCAGA 19950 0.0 26.166609 7 CGCAGAG 20100 0.0 25.852198 8 TAAGGTG 2020 0.0 24.70094 5 GTGTAAG 1495 0.0 24.588768 1 AGGTAAG 1920 0.0 23.512453 2 TAAGGTA 1095 0.0 23.434473 4 GCAGAGT 21825 0.0 23.306313 9 GGTAAGG 2020 0.0 23.054209 3 GAGTACT 13120 0.0 22.75233 12-13 CGCGCGT 230 3.594323E-9 22.738033 7 GTAAGGT 2055 0.0 22.661558 4 TAGCGCG 235 4.634785E-9 22.24336 4 GTACTTT 13635 0.0 21.704926 14-15 >>END_MODULE