FastQCFastQC Report
Thu 26 May 2016
SRR938289_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938289_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2968039
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT148300.49965650720896865No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT124490.4194351893624039No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80800.2722336195717105No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC42620.1435964958681473No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA38010.12806435494951382No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA36100.12162912953637066No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA36070.12152805269742076No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA33720.11361036697967916No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31780.10707406472758613No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA30390.1023908378562411No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA74100.051.9260061
GTATCAA124700.039.1220971
TATCAAC160700.030.0924342
ATCAACG160900.030.0524963
TCAACGC162750.029.7103884
CAACGCA166950.028.9629545
AACGCAG171000.028.3047686
TAAGGTG20700.026.3881555
AGGTAAG19250.024.9239032
ACGCAGA193600.024.8779247
CGCAGAG195600.024.6235478
GGTAAGG20900.024.5451853
GTAAGGT21450.024.1368584
GAGTACT132100.022.49047912-13
GCAGAGT214100.022.3623839
TGGTATC35700.022.2215162
GTGTAAG12450.022.1297631
GTGGTAT37750.021.769441
GTACTTT136450.021.70386714-15
AAGGTAA21300.021.6326751