##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938289_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2968039 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.51484195457 34.0 31.0 34.0 31.0 34.0 2 32.733800667713595 34.0 31.0 34.0 31.0 34.0 3 32.78838519305171 34.0 31.0 34.0 31.0 34.0 4 36.246739345406176 37.0 35.0 37.0 35.0 37.0 5 36.20721998599075 37.0 37.0 37.0 35.0 37.0 6 36.24624204735854 37.0 37.0 37.0 35.0 37.0 7 36.21298406119327 37.0 37.0 37.0 35.0 37.0 8 36.2068783462751 37.0 37.0 37.0 35.0 37.0 9 37.9220246095149 39.0 38.0 39.0 35.0 39.0 10-11 37.9070953919406 39.0 38.0 39.0 35.0 39.0 12-13 37.85803993815445 39.0 38.0 39.0 35.0 39.0 14-15 39.1726889370389 40.5 38.5 41.0 36.0 41.0 16-17 39.12835124471073 40.0 38.5 41.0 36.0 41.0 18-19 39.162863425985975 40.0 39.0 41.0 36.0 41.0 20-21 39.09176345054765 40.0 39.0 41.0 36.0 41.0 22-23 38.95137850277574 40.0 38.0 41.0 35.0 41.0 24-25 38.9054304879417 40.0 38.0 41.0 35.0 41.0 26-27 38.864088544658614 40.0 38.0 41.0 35.0 41.0 28-29 38.80543668732116 40.0 38.0 41.0 35.0 41.0 30-31 38.59685890246051 40.0 38.0 41.0 34.5 41.0 32-33 38.403881148462 40.0 38.0 41.0 34.0 41.0 34-35 38.20030161328742 40.0 38.0 41.0 34.0 41.0 36-37 38.0559426611308 40.0 38.0 41.0 33.5 41.0 38-39 38.02896828512024 40.0 38.0 41.0 33.0 41.0 40-41 37.95680767671853 40.0 38.0 41.0 33.0 41.0 42-43 37.78816989938474 40.0 37.5 41.0 33.0 41.0 44-45 37.565380710967744 40.0 37.0 41.0 33.0 41.0 46-47 37.22237797414387 40.0 37.0 41.0 32.0 41.0 48-49 36.85324064137971 40.0 36.5 41.0 31.0 41.0 50-51 36.53315421394396 40.0 36.0 41.0 30.5 41.0 52-53 36.109609745694044 39.0 35.0 41.0 29.5 41.0 54-55 35.87465646509362 39.0 35.0 41.0 29.0 41.0 56-57 35.61718764477151 39.0 35.0 40.0 28.0 41.0 58-59 35.26404791176935 38.0 35.0 40.0 28.0 41.0 60-61 34.895945774297445 38.0 34.0 40.0 27.0 41.0 62-63 34.52228188376231 37.0 34.0 40.0 26.0 41.0 64-65 34.12291347923663 36.5 34.0 39.5 26.0 41.0 66-67 33.698590550865404 36.0 33.5 39.0 25.5 41.0 68-69 33.23571388381352 35.5 33.0 39.0 24.5 40.0 70-71 32.71852728350268 35.0 32.5 38.0 22.5 40.0 72-73 32.301015081001296 35.0 32.0 37.0 22.0 39.5 74-75 31.861808756556094 35.0 31.5 37.0 20.5 39.0 76-77 30.572783578652437 33.5 30.0 35.5 20.0 37.5 78-79 31.298422797005024 34.5 31.0 36.0 20.0 38.0 80-81 31.152669321393688 35.0 31.0 35.5 20.0 37.0 82-83 30.795002188313564 34.5 31.0 35.0 18.0 37.0 84-85 30.42086407894236 34.0 31.0 35.0 16.0 36.5 86-87 30.00636042855232 34.0 30.5 35.0 9.0 36.0 88-89 29.549206395198986 34.0 30.0 35.0 6.0 36.0 90-91 29.211044059731023 34.0 29.5 35.0 2.0 35.0 92-93 28.80719020201554 34.0 29.0 35.0 2.0 35.0 94-95 28.39268570931851 34.0 29.0 35.0 2.0 35.0 96-97 27.812462538396566 34.0 28.0 35.0 2.0 35.0 98-99 27.11842752066263 33.0 27.0 35.0 2.0 35.0 100-101 24.658884704682116 31.0 21.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.09583474002022285 1101 2 0.10596074748447393 1101 3 0.1722651973746352 1101 4 0.04410334617163869 1101 5 0.10800825415817172 1101 6 0.06920956480235674 1101 7 0.10950679498439087 1101 8 0.12731370016638266 1101 9 0.048929683813263125 1101 10-11 0.13248957433180664 1101 12-13 0.17666178519562692 1101 14-15 0.18005989994025384 1101 16-17 0.13682750545945765 1101 18-19 0.20282462072137264 1101 20-21 0.15904806401953664 1101 22-23 0.2531967006647662 1101 24-25 0.15681306227271108 1101 26-27 0.14051785224858548 1101 28-29 0.20336874902348967 1101 30-31 0.15419402965969198 1101 32-33 0.08021005660561542 1101 34-35 0.049102373202067895 1101 36-37 0.07167795223493556 1101 38-39 0.09084880305635323 1101 40-41 0.20919473384903853 1101 42-43 0.20189261018781224 1101 44-45 0.2524944604084496 1101 46-47 0.20561100381515018 1101 48-49 0.24174792773278853 1101 50-51 0.14027618355076044 1101 52-53 0.1310776730767813 1101 54-55 0.16437751451030636 1101 56-57 0.30700995347803683 1101 58-59 0.22218154106550259 1101 60-61 0.16637462921554658 1101 62-63 0.10390603103743246 1101 64-65 0.24432855313941815 1101 66-67 0.15911940729132112 1101 68-69 0.22367273149435363 1101 70-71 0.19536113401015598 1101 72-73 0.22821910951916635 1101 74-75 0.14255622199524254 1101 76-77 0.10604176564432066 1101 78-79 0.12955108742464816 1101 80-81 0.30643483874927213 1101 82-83 0.4033620684032897 1101 84-85 0.3904413502865154 1101 86-87 0.4924592998134827 1101 88-89 0.4527456711785405 1101 90-91 0.46530032873768334 1101 92-93 0.5505958826383939 1101 94-95 0.6302458155692854 1101 96-97 0.659523110909987 1101 98-99 0.7200593230461578 1101 100-101 0.9498609459169103 1102 1 0.10676070486312028 1102 2 0.15232608446162033 1102 3 0.1285261649412277 1102 4 0.09889026687648084 1102 5 0.08632184476324056 1102 6 0.08837385876520898 1102 7 0.13378078899787482 1102 8 0.14449943550948774 1102 9 0.0912852917441711 1102 10-11 0.10660293977016266 1102 12-13 0.10717474144417594 1102 14-15 0.162644505193569 1102 16-17 0.1360304218087549 1102 18-19 0.13762314853661195 1102 20-21 0.13573246204736478 1102 22-23 0.15550780728318614 1102 24-25 0.12560458089478033 1102 26-27 0.0380732801170538 1102 28-29 0.060031502317563934 1102 30-31 0.022322166601725257 1102 32-33 0.06274426684179701 1102 34-35 0.14418772107989497 1102 36-37 0.1122908679605672 1102 38-39 0.13556869099911495 1102 40-41 0.28213413184582947 1102 42-43 0.16841044160007357 1102 44-45 0.1816519975965818 1102 46-47 0.14675797371498334 1102 48-49 0.16581106071858898 1102 50-51 0.2458193841988887 1102 52-53 0.3076321081190798 1102 54-55 0.3093933882017694 1102 56-57 0.2913840990903722 1102 58-59 0.2217685531611977 1102 60-61 0.27300767752884525 1102 62-63 0.19432326335491013 1102 64-65 0.38139383700227825 1102 66-67 0.3434963756157643 1102 68-69 0.558321359996178 1102 70-71 0.5382379682927194 1102 72-73 0.4521949601087343 1102 74-75 0.463794233966496 1102 76-77 0.3965545563725854 1102 78-79 0.36700960020710127 1102 80-81 0.5302234445097938 1102 82-83 0.4719284055367936 1102 84-85 0.508760870064858 1102 86-87 0.41529499639232625 1102 88-89 0.11632235480808006 1102 90-91 0.265877418187511 1102 92-93 0.3762483355456574 1102 94-95 0.3386736949794802 1102 96-97 0.41785432351459306 1102 98-99 0.5263641534898333 1102 100-101 0.5183561979969227 1103 1 0.12145064139605921 1103 2 0.12123972675409789 1103 3 0.12238782091892375 1103 4 0.06325203328602669 1103 5 0.0711735118674639 1103 6 0.06620664410652921 1103 7 0.08517993971442905 1103 8 0.12144918276640482 1103 9 0.11032082579676938 1103 10-11 0.11282212824190907 1103 12-13 0.0955343908959776 1103 14-15 0.13370948496162782 1103 16-17 0.15040531502324228 1103 18-19 0.16098615261633853 1103 20-21 0.12014927769285322 1103 22-23 0.21306968037406904 1103 24-25 0.21399631809197928 1103 26-27 0.19242158634249762 1103 28-29 0.23654848173556076 1103 30-31 0.24840616981883556 1103 32-33 0.22234287831754074 1103 34-35 0.15319291762775578 1103 36-37 0.29620865665651763 1103 38-39 0.219927998737262 1103 40-41 0.32082841064249834 1103 42-43 0.34932094179384876 1103 44-45 0.29412579607720346 1103 46-47 0.264874006336143 1103 48-49 0.36975306107697037 1103 50-51 0.36404730720497014 1103 52-53 0.33722555221764594 1103 54-55 0.3921762291757389 1103 56-57 0.3324514125126399 1103 58-59 0.3445898160848486 1103 60-61 0.27458997842855837 1103 62-63 0.4077834474635651 1103 64-65 0.24152377153495053 1103 66-67 0.37710495512877884 1103 68-69 0.3887778394287622 1103 70-71 0.36934224513078107 1103 72-73 0.2837878457971854 1103 74-75 0.256520317136264 1103 76-77 0.31917433325857303 1103 78-79 0.23033893299036023 1103 80-81 0.3137320102709147 1103 82-83 0.41779867103163326 1103 84-85 0.5194663003948641 1103 86-87 0.577525636438665 1103 88-89 0.4224700751196231 1103 90-91 0.37174655852764005 1103 92-93 0.36068598738944857 1103 94-95 0.16511858115132938 1103 96-97 0.16306220626513834 1103 98-99 0.08982683794227952 1103 100-101 0.28472657392361 1104 1 0.09324245218508764 1104 2 0.1081342853060363 1104 3 0.09850304738233717 1104 4 0.05741188921468421 1104 5 0.0954967744915649 1104 6 0.07404052331923339 1104 7 0.03973267574348682 1104 8 0.06207255660704902 1104 9 0.0423075716414516 1104 10-11 0.024474867331974792 1104 12-13 0.09102374369731336 1104 14-15 0.12073349855248239 1104 16-17 0.09760870355670903 1104 18-19 0.14551557398419845 1104 20-21 0.12191012130606538 1104 22-23 0.10878235906028522 1104 24-25 0.09832307536442642 1104 26-27 0.045080707629750805 1104 28-29 0.10731026405724009 1104 30-31 0.023695052245599868 1104 32-33 -0.03455037930691418 1104 34-35 0.1111313812068957 1104 36-37 0.16313385077530995 1104 38-39 0.09267293022175238 1104 40-41 0.12003816096645181 1104 42-43 0.20185583726049572 1104 44-45 0.18095211970715042 1104 46-47 0.08544983255264071 1104 48-49 -0.007394489608181232 1104 50-51 0.024165462030644846 1104 52-53 0.014062283267463727 1104 54-55 -0.003030490752429671 1104 56-57 0.12318141139026295 1104 58-59 0.22775135046504857 1104 60-61 0.12156634236173858 1104 62-63 0.016143437056136634 1104 64-65 0.04489444913960483 1104 66-67 -0.050488992242328834 1104 68-69 0.11753547918919338 1104 70-71 0.07516644646334214 1104 72-73 0.03391245424351297 1104 74-75 0.028987717423998305 1104 76-77 0.00925080738797135 1104 78-79 0.12694210929409522 1104 80-81 0.04309305080883874 1104 82-83 0.12534354027566508 1104 84-85 0.22026560554653685 1104 86-87 0.16943233698046356 1104 88-89 0.10370355844224477 1104 90-91 0.1433441229873189 1104 92-93 0.23741239468688846 1104 94-95 0.09836952241062491 1104 96-97 0.2880400139727506 1104 98-99 0.3876836236820722 1104 100-101 0.5306155155298242 1105 1 0.09978287246545392 1105 2 0.09515443269872748 1105 3 0.06337995810283559 1105 4 0.02346466435891159 1105 5 0.08370805881207843 1105 6 0.015850743631617092 1105 7 0.020181739456113235 1105 8 0.033686180722547476 1105 9 0.039631331679871096 1105 10-11 0.004174276037446134 1105 12-13 0.07870238463861057 1105 14-15 0.10421308880422941 1105 16-17 0.05448406524275384 1105 18-19 0.04360357486314115 1105 20-21 0.10188289599566502 1105 22-23 0.027789014083545283 1105 24-25 0.10628034597614544 1105 26-27 0.045245686191414336 1105 28-29 -0.045294491113871516 1105 30-31 0.0732653976362414 1105 32-33 0.09460593204948253 1105 34-35 0.0924263522199098 1105 36-37 0.05282404625388892 1105 38-39 -0.01406961987443367 1105 40-41 0.06813390464979108 1105 42-43 0.107399602337658 1105 44-45 0.024468444474933904 1105 46-47 0.14375942444291212 1105 48-49 0.12612389753960684 1105 50-51 0.06742098215293879 1105 52-53 0.055266374593728074 1105 54-55 0.14031782463116826 1105 56-57 0.13776831028108205 1105 58-59 0.1271343768171036 1105 60-61 0.14671453300805837 1105 62-63 0.30780736098628125 1105 64-65 0.09368003644197387 1105 66-67 0.16233654887788163 1105 68-69 0.0635622129380593 1105 70-71 0.12663070075313954 1105 72-73 0.03225538754931989 1105 74-75 0.04479671262109264 1105 76-77 0.24442688870493967 1105 78-79 0.17496673520061634 1105 80-81 0.12954021883669142 1105 82-83 0.34496972620336663 1105 84-85 0.38191553086517516 1105 86-87 0.4454725484182198 1105 88-89 0.3619381611535033 1105 90-91 0.24797302955179745 1105 92-93 0.18594109610256737 1105 94-95 0.14373466075346641 1105 96-97 0.22915918307275618 1105 98-99 0.21338433564122639 1105 100-101 0.3928488268265511 1106 1 -0.15200033476402552 1106 2 -0.07590392426696724 1106 3 -0.0315786550929289 1106 4 -0.024077050318027204 1106 5 -0.07227817134039327 1106 6 -0.05638948648147846 1106 7 -0.03602640783800837 1106 8 -0.18542927572058687 1106 9 -0.13130382114450612 1106 10-11 -0.08170321321875207 1106 12-13 -0.1537295971760031 1106 14-15 -0.3610953884582173 1106 16-17 -0.25296916352902343 1106 18-19 -0.11120237112992015 1106 20-21 -0.043710719404536746 1106 22-23 -0.06802293172091112 1106 24-25 -0.015078726966812894 1106 26-27 -0.1041396805878847 1106 28-29 -0.07518485209394044 1106 30-31 -0.04600219864981625 1106 32-33 -0.004479041155917685 1106 34-35 0.024464760132246965 1106 36-37 -0.039635192461972224 1106 38-39 -0.0182548760693777 1106 40-41 -0.02192596219992282 1106 42-43 -0.09217066852607303 1106 44-45 -0.01454397415707831 1106 46-47 -0.12617445868794164 1106 48-49 -0.1923678465739087 1106 50-51 -0.2159986881136291 1106 52-53 -0.1903445354438702 1106 54-55 -0.438242144639176 1106 56-57 -0.2748618183359781 1106 58-59 -0.2029409746054469 1106 60-61 -0.25705462710701 1106 62-63 -0.22816732712588816 1106 64-65 -0.3383721311852028 1106 66-67 -0.42556315788474564 1106 68-69 -0.5015969455108689 1106 70-71 -0.5264528052866808 1106 72-73 -0.32829963213858804 1106 74-75 -0.3035430194594113 1106 76-77 -0.23204209902795725 1106 78-79 -0.377806319431901 1106 80-81 -0.5628123619388781 1106 82-83 -0.6131632612679248 1106 84-85 -0.4195975311342579 1106 86-87 -0.2062721776673122 1106 88-89 -0.17517812271482214 1106 90-91 -0.3863490765067006 1106 92-93 -0.2574149939184416 1106 94-95 -0.4977256859936503 1106 96-97 -0.36776396045672755 1106 98-99 -0.04136544308068224 1106 100-101 -0.13711574449845898 1107 1 0.11597580780734518 1107 2 0.12242987705457153 1107 3 0.07044569424370195 1107 4 0.07801544306722974 1107 5 0.04056935919926019 1107 6 0.016961227601321127 1107 7 0.06441233246995637 1107 8 0.0899465217263753 1107 9 0.0412023997388431 1107 10-11 0.09550302978401959 1107 12-13 0.12766856751414224 1107 14-15 0.21244693967772577 1107 16-17 0.11445905487769181 1107 18-19 0.09082955950600535 1107 20-21 0.13036095213940513 1107 22-23 0.1684689074814827 1107 24-25 0.12207886425282055 1107 26-27 0.08918378025772 1107 28-29 0.13091775421872143 1107 30-31 0.13361313367347805 1107 32-33 0.1945010878422977 1107 34-35 0.2814809403997671 1107 36-37 0.2057998957851197 1107 38-39 0.1903987498129993 1107 40-41 0.27499743260511167 1107 42-43 0.36932531464519514 1107 44-45 0.3840459589510985 1107 46-47 0.3224444904190733 1107 48-49 0.28931493455343826 1107 50-51 0.38285981142673364 1107 52-53 0.5024961653530298 1107 54-55 0.4411606374128212 1107 56-57 0.4849991020710007 1107 58-59 0.5047932544021663 1107 60-61 0.38534956285362654 1107 62-63 0.4149197275535741 1107 64-65 0.46342263186797084 1107 66-67 0.3677400021695192 1107 68-69 0.546112379147651 1107 70-71 0.5114754381536528 1107 72-73 0.49723851138307396 1107 74-75 0.5073200512100158 1107 76-77 0.48717631941002537 1107 78-79 0.6088116465164539 1107 80-81 0.6654623676952234 1107 82-83 0.6213094544356537 1107 84-85 0.7214978986898082 1107 86-87 0.7849978794980963 1107 88-89 0.7939003648773166 1107 90-91 0.8341377853316772 1107 92-93 0.9389719363159692 1107 94-95 0.9293525678052426 1107 96-97 1.0087044643629106 1107 98-99 0.9668779600111925 1107 100-101 0.9687733693583063 1108 1 0.07119992124242458 1108 2 0.055398124990169606 1108 3 0.014418120716214844 1108 4 0.061619685051738315 1108 5 0.07269205804620782 1108 6 0.051679100452766136 1108 7 0.09751679379886014 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-0.20455390047662192 2316 98-99 -0.19368566733266945 2316 100-101 -0.22982150287356973 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 10.0 4 23.0 5 39.0 6 94.0 7 231.0 8 560.0 9 1301.0 10 2126.0 11 3398.0 12 5517.0 13 7634.0 14 10685.0 15 14589.0 16 19363.0 17 27534.0 18 27171.0 19 19313.0 20 16218.0 21 15427.0 22 16016.0 23 17087.0 24 19218.0 25 22438.0 26 26527.0 27 32494.0 28 39404.0 29 47854.0 30 58569.0 31 72504.0 32 91687.0 33 120812.0 34 169990.0 35 262596.0 36 439043.0 37 708684.0 38 588156.0 39 63716.0 40 8.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.767665788758165 18.968753442929824 15.557612282048854 22.705968486263153 2 15.787503264449928 19.975232302469188 39.65891342257567 24.578351010505212 3 19.314943315715595 23.784183928928833 29.82638563643213 27.074487118923447 4 13.345511969350806 17.03653489728403 36.23742814700211 33.38052498636305 5 13.999652295556864 36.94275477605071 33.880496139200375 15.177096789192051 6 32.415207482111924 36.0151601781513 16.75843882105323 14.811193518683549 7 28.807134946676914 32.85074758114701 19.99175212994169 18.350365342234383 8 26.909783991547258 34.20829020711367 19.277858190208192 19.604067611130876 9 26.305791692595786 16.83963791434512 20.07764754161603 36.77692285144306 10-11 25.199365641758753 26.49036956724625 26.425292929102344 21.884971861892648 12-13 26.33420585106867 23.727787943487264 27.085307841305315 22.852698364138746 14-15 22.988983635322853 25.063720523887994 25.72926096995356 26.218034870835595 16-17 22.189113864001467 28.27141014652133 26.698381284298822 22.84109470517838 18-19 22.28653329690075 26.371604281480128 29.346042959678094 21.995819461941032 20-21 22.677717509776656 25.51231638128744 29.902656265635326 21.907309843300578 22-23 22.560148299938106 25.36833242420332 29.867633140939187 22.203886134919387 24-25 22.498906635057356 25.638178080003986 29.719225904359376 22.14368938057928 26-27 21.898761051210894 26.052853727208852 29.59050493102629 22.457880290553963 28-29 22.10889820143812 26.42589144748147 29.405654509814227 22.059555841266185 30-31 22.104561294511292 25.923968654050704 29.34050057967567 22.630969471762334 32-33 21.869359679005616 25.824158607721557 29.43263319370073 22.873848519572093 34-35 22.7413472531009 25.969790891774423 29.11290968017503 22.175952174949643 36-37 22.807778601769513 25.54208529358945 29.110809966653605 22.53932613798743 38-39 22.223923989954518 25.96803204041109 29.05690896637829 22.751135003256103 40-41 22.728632873207808 25.777531712537687 29.133935346994672 22.35990006725983 42-43 22.437067040546182 25.822620856317524 28.87825026004102 22.862061843095276 44-45 22.358891611682008 26.107675704670125 29.066250801479406 22.46718188216846 46-47 22.88477341436551 26.431374385579165 28.201987911883908 22.481864288171415 48-49 22.622024171515267 26.50482355521609 28.039388970293178 22.833763302975466 50-51 22.787099495660264 26.707988001505374 28.051602421666296 22.45331008116807 52-53 23.169624792666134 26.40677902143469 27.950323429038498 22.473272756860673 54-55 22.655126836271357 26.67795133419743 28.047256117591445 22.619665711939767 56-57 22.697494877931188 26.757161883654497 27.868872342984712 22.67647089542961 58-59 22.680850891784104 26.375866354855848 27.860836734288196 23.08244601907185 60-61 22.31966965393649 26.70941992339049 28.127864896653985 22.843045526019033 62-63 22.38466206138127 26.687856864414517 28.328923575465147 22.598557498739066 64-65 22.117044284121604 26.737603515317687 28.107750605702957 23.037601594857748 66-67 22.14586802936215 26.707347848192022 28.050760114675043 23.096024007770787 68-69 22.62109884334053 26.410211487080655 28.195419183768355 22.773270485810453 70-71 22.55495968887201 26.59607909464802 27.644178529999103 23.204782686480872 72-73 22.498424043619373 26.680528793590653 27.687388878650182 23.133658284139795 74-75 22.3765253758458 27.180320069918217 27.47447051740223 22.968684036833746 76-77 22.587135815937728 26.716141533180664 27.76001258743568 22.93671006344593 78-79 22.57199113623507 26.942132498932796 27.700461483154363 22.785414881677767 80-81 22.790586646604037 27.014284515803194 27.330301252780032 22.864827584812733 82-83 22.794010252899348 27.018635371475135 27.39543304441637 22.791921331209146 84-85 22.60577269242322 27.24502532297896 27.3950780612856 22.75412392331222 86-87 22.85225842524192 27.18337968561981 27.06311474898927 22.901247140148996 88-89 22.671297561317463 27.35902551080057 26.950017806381794 23.01965912150017 90-91 22.60311034172789 27.291314722135933 27.109982567585146 22.995592368551034 92-93 22.724892824750103 26.924454420364174 27.24387440044528 23.106778354440443 94-95 22.511311340585486 26.735885209055542 27.453513919459954 23.29928953089902 96-97 22.752278524642026 26.50221240354321 27.44037393039647 23.30513514141829 98-99 22.497379582950224 26.7993109254966 27.102255057969256 23.601054433583926 100-101 22.589527967792876 26.4725295051716 27.410573109046073 23.527369417989455 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1314.0 1 872.5 2 609.0 3 819.0 4 684.5 5 615.0 6 1230.5 7 2989.5 8 4934.0 9 5650.0 10 4720.0 11 3487.0 12 3276.5 13 3404.0 14 3610.0 15 3918.0 16 4148.5 17 4366.0 18 4708.0 19 5234.5 20 5864.0 21 6781.0 22 8105.0 23 9871.0 24 12056.5 25 14809.5 26 18670.5 27 23110.5 28 26337.0 29 29570.0 30 33862.0 31 39108.5 32 47399.0 33 50925.0 34 50132.0 35 53058.5 36 59396.0 37 65745.0 38 69925.5 39 73563.0 40 77939.5 41 83180.5 42 85993.0 43 88475.5 44 92875.0 45 98998.0 46 102195.5 47 105631.0 48 108940.5 49 107595.0 50 106223.5 51 103857.0 52 102783.5 53 106561.5 54 111399.5 55 107091.5 56 98091.5 57 89561.0 58 83273.5 59 75451.5 60 60785.0 61 50161.5 62 43111.0 63 31775.5 64 21530.5 65 16249.5 66 12313.0 67 9557.5 68 7362.0 69 5682.0 70 4437.0 71 3049.5 72 2155.0 73 1742.5 74 1254.0 75 862.5 76 657.0 77 445.5 78 267.5 79 135.5 80 72.0 81 34.5 82 22.5 83 17.0 84 8.0 85 2.5 86 1.5 87 2.0 88 3.0 89 2.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.015633217757583376 3 0.006637379091042942 4 0.0 5 3.3692279649964164E-5 6 0.0 7 0.0 8 2.3584595754974917E-4 9 4.0430735579957E-4 10-11 0.0 12-13 0.0 14-15 0.0 16-17 2.3584595754974917E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0046158423120450905 26-27 0.02035013690857836 28-29 3.3692279649964164E-5 30-31 0.0 32-33 4.0430735579957E-4 34-35 0.01874975362520506 36-37 0.003436612524296345 38-39 0.018075908032205778 40-41 0.014925679884934127 42-43 0.008018762556691473 44-45 0.003032305168496775 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 3.032305168496775E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 1.6846139824982082E-5 84-85 0.002779613071122044 86-87 2.863843770246954E-4 88-89 6.738455929992833E-5 90-91 1.6846139824982086E-4 92-93 1.010768389498925E-4 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2968039.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.91177358633994 #Duplication Level Percentage of deduplicated Percentage of total 1 71.1674985436842 32.67426079843806 2 15.032859187413713 13.803704547357373 3 5.452843558238174 7.510491565426899 4 2.610408957267235 4.793940200552281 5 1.4949674282997047 3.4318303043524465 6 0.8942938861655463 2.463517105276838 7 0.5953623137473028 1.9133897825423956 8 0.4446282349247024 1.633093668156552 9 0.3241145569632444 1.3392606739800024 >10 1.7753318535972815 14.975859935888824 >50 0.13378211803338103 4.240111714440403 >100 0.06621091041513918 5.583154016149796 >500 0.0035192920002793697 1.1092929996628769 >1k 0.003959203500221981 3.337842910073948 >5k 7.331858333744408E-5 0.27198784478708227 >10k+ 1.4663716667488816E-4 0.91826193291421 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14830 0.49965650720896865 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12449 0.4194351893624039 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8080 0.2722336195717105 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 4262 0.1435964958681473 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 3801 0.12806435494951382 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 3610 0.12162912953637066 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 3607 0.12152805269742076 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 3372 0.11361036697967916 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3178 0.10707406472758613 No Hit GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 3039 0.1023908378562411 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 6.738455929992834E-5 2 1.010768389498925E-4 0.0 0.0 0.0 6.738455929992834E-5 3 1.010768389498925E-4 0.0 0.0 0.0 6.738455929992834E-5 4 1.010768389498925E-4 0.0 0.0 0.0 6.738455929992834E-5 5 1.010768389498925E-4 0.0 0.0 0.0 1.3476911859985668E-4 6 1.010768389498925E-4 0.0 0.0 0.0 1.6846139824982083E-4 7 1.010768389498925E-4 0.0 0.0 0.0 1.6846139824982083E-4 8 1.010768389498925E-4 0.0 0.0 0.0 2.02153677899785E-4 9 1.010768389498925E-4 0.0 0.0 0.0 2.02153677899785E-4 10-11 1.010768389498925E-4 0.0 0.0 3.369227964996417E-5 2.02153677899785E-4 12-13 1.010768389498925E-4 0.0 0.0 3.369227964996417E-5 3.874612159745879E-4 14-15 1.6846139824982083E-4 0.0 0.0 5.053841947494626E-5 4.885380549244805E-4 16-17 2.02153677899785E-4 0.0 0.0 6.738455929992834E-5 5.222303345744446E-4 18-19 2.1899981772476707E-4 0.0 0.0 6.738455929992834E-5 5.89614893874373E-4 20-21 2.3584595754974917E-4 0.0 0.0 8.423069912491041E-5 6.906917328242655E-4 22-23 2.3584595754974917E-4 0.0 0.0 1.3476911859985668E-4 7.075378726492475E-4 24-25 2.3584595754974917E-4 0.0 0.0 3.5376893632462376E-4 7.075378726492475E-4 26-27 2.3584595754974917E-4 0.0 0.0 8.0861471159914E-4 7.749224319491759E-4 28-29 2.863843770246954E-4 0.0 0.0 0.002560613253397277 7.749224319491759E-4 30-31 3.7061507614960586E-4 0.0 0.0 0.009416992162164985 7.749224319491759E-4 32-33 3.7061507614960586E-4 0.0 0.0 0.024443748886049006 8.423069912491042E-4 34-35 3.874612159745879E-4 0.0 0.0 0.04639426907800066 9.265376903740146E-4 36-37 4.0430735579957E-4 0.0 0.0 0.08062562520236426 9.602299700239787E-4 38-39 4.379996354495342E-4 0.0 0.0 0.13227588990575934 0.001010768389498925 40-41 4.885380549244805E-4 0.0 0.0 0.1847516154605785 0.001010768389498925 42-43 5.390764743994267E-4 0.0 0.0 0.23978795426879498 0.001010768389498925 44-45 5.727687540493908E-4 0.0 0.0 0.3073578211067981 0.0010613068089738713 46-47 5.727687540493908E-4 0.0 0.0 0.38916267609691113 0.0011286913682737997 48-49 5.727687540493908E-4 0.0 0.0 0.4717256073791483 0.001179229787748746 50-51 5.727687540493908E-4 0.0 0.0 0.5629137622517764 0.0013139989063486025 52-53 5.727687540493908E-4 0.0 0.0 0.650783227578883 0.0014487680249484591 54-55 5.727687540493908E-4 0.0 0.0 0.7451216105987826 0.0014824603045984234 56-57 6.401533133493192E-4 0.0 0.0 0.851909290949344 0.0014993064444234054 58-59 6.401533133493192E-4 0.0 0.0 0.9688888858940196 0.0015161525842483875 60-61 6.569994531743013E-4 0.0 0.0 1.094510550568911 0.0015161525842483875 62-63 6.738455929992833E-4 0.0 0.0 1.2162912953637064 0.0015161525842483875 64-65 6.738455929992833E-4 0.0 0.0 1.344540957851295 0.0015161525842483875 66-67 6.738455929992833E-4 0.0 0.0 1.4881037614397923 0.0015161525842483875 68-69 7.075378726492475E-4 0.0 0.0 1.6495234732427706 0.0015161525842483875 70-71 7.075378726492475E-4 0.0 0.0 1.816755103285368 0.001566691003723334 72-73 7.075378726492475E-4 0.0 0.0 1.9891416521144096 0.0017351524019731546 74-75 7.075378726492475E-4 0.0 0.0 2.1668684272679704 0.0018193831010980652 76-77 7.075378726492475E-4 0.0 0.0 2.3514515813302994 0.0018362292409230471 78-79 7.075378726492475E-4 0.0 0.0 2.5600573307830525 0.0018530753807480293 80-81 7.749224319491759E-4 0.0 0.0 2.7823758380533405 0.0018699215205730114 82-83 8.423069912491042E-4 0.0 0.0 3.018171257183615 0.00202153677899785 84-85 8.928454107240504E-4 0.0 0.0 3.255331213639713 0.00202153677899785 86-87 9.265376903740146E-4 0.0 0.0 3.5052268518034975 0.002038382918822832 88-89 9.433838301989967E-4 0.0 0.0 3.7649437894852458 0.0020552290586478145 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7410 0.0 51.926006 1 GTATCAA 12470 0.0 39.122097 1 TATCAAC 16070 0.0 30.092434 2 ATCAACG 16090 0.0 30.052496 3 TCAACGC 16275 0.0 29.710388 4 CAACGCA 16695 0.0 28.962954 5 AACGCAG 17100 0.0 28.304768 6 TAAGGTG 2070 0.0 26.388155 5 AGGTAAG 1925 0.0 24.923903 2 ACGCAGA 19360 0.0 24.877924 7 CGCAGAG 19560 0.0 24.623547 8 GGTAAGG 2090 0.0 24.545185 3 GTAAGGT 2145 0.0 24.136858 4 GAGTACT 13210 0.0 22.490479 12-13 GCAGAGT 21410 0.0 22.362383 9 TGGTATC 3570 0.0 22.221516 2 GTGTAAG 1245 0.0 22.129763 1 GTGGTAT 3775 0.0 21.76944 1 GTACTTT 13645 0.0 21.703867 14-15 AAGGTAA 2130 0.0 21.632675 1 >>END_MODULE