FastQCFastQC Report
Thu 26 May 2016
SRR938288_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938288_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3012008
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT154190.5119176310288684No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT122110.4054106097991772No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76700.2546473980148791No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC40510.1344949947012093No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA37180.12343924717331427No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA36190.1201524033136698No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA35560.11806077540298697No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA33910.11258270230357953No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31450.10441539331900845No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA31320.1039837875596612No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA80200.049.3231851
GTATCAA135000.040.2676961
TATCAAC170600.031.6410392
ATCAACG171700.031.3008793
TCAACGC173400.030.9391484
CAACGCA176550.030.3871365
AACGCAG181750.029.5128256
ACGCAGA203000.026.254217
CGCAGAG204750.025.8414758
GCAGAGT216550.023.6418729
GAGTACT130800.023.54749112-13
AGGTAAG20400.023.31332
GTGTAAG12900.022.8784431
GTACTTT136350.022.5354114-15
TAAGGTG22150.022.3309925
GTAAGGT23200.021.7303264
AGTACTT134900.021.54576512-13
AGAGTAC188000.021.49063710-11
TACATGG109100.021.3601972
CAGAGTA206450.021.31985910-11
Produced by FastQC (version 0.11.5)