FastQCFastQC Report
Thu 26 May 2016
SRR938288_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938288_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3012008
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT149580.4966122267935543No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT123380.4096270660635695No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79630.26437512782170564No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC43320.14382431919171532No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA37220.12357204894542113No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA37100.12317364362910059No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA36600.121513621477765No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA34300.11387751958162129No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32110.10660662255877142No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA31620.10497980085046255No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA74450.051.806221
GTATCAA127850.040.1620181
TATCAAC162650.031.5096442
ATCAACG163350.031.284273
TCAACGC165100.030.8638064
CAACGCA168250.030.2859695
AACGCAG173400.029.4686366
TAAGGTG19800.028.5416225
GTAAGGT21000.026.9106714
ACGCAGA196900.025.8305347
CGCAGAG196500.025.8106148
GGTAAGG21550.024.6833233
AGGTAAG21600.024.6286372
GTGGTAT39400.023.8703921
TGGTATC37400.023.3680462
GAGTACT132650.023.30731212-13
GCAGAGT215500.023.2925669
GGACCGA8900.022.9443246
AAGGTAA23350.022.3767171
GTACTTT139050.021.92716614-15
Produced by FastQC (version 0.11.5)