##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938288_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3012008 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.532372091973194 34.0 31.0 34.0 31.0 34.0 2 32.74716767020539 34.0 31.0 34.0 31.0 34.0 3 32.79821700340769 34.0 31.0 34.0 31.0 34.0 4 36.25172277098866 37.0 35.0 37.0 35.0 37.0 5 36.2245253000656 37.0 37.0 37.0 35.0 37.0 6 36.258087627921306 37.0 37.0 37.0 35.0 37.0 7 36.22085631910672 37.0 37.0 37.0 35.0 37.0 8 36.20420098485794 37.0 37.0 37.0 35.0 37.0 9 37.89927516792784 39.0 38.0 39.0 35.0 39.0 10-11 37.875530377077354 39.0 38.0 39.0 35.0 39.0 12-13 37.79774937516766 39.0 38.0 39.0 35.0 39.0 14-15 39.07169270466745 40.0 38.5 41.0 36.0 41.0 16-17 38.958084274676565 40.0 38.0 41.0 36.0 41.0 18-19 39.215900488976125 40.5 39.0 41.0 36.0 41.0 20-21 39.19782367775916 40.5 39.0 41.0 36.0 41.0 22-23 39.10329720239787 40.0 39.0 41.0 35.5 41.0 24-25 38.998929617716826 40.0 38.5 41.0 35.0 41.0 26-27 38.82002239037878 40.0 38.0 41.0 35.0 41.0 28-29 38.7035303359088 40.0 38.0 41.0 34.5 41.0 30-31 38.44453915793052 40.0 38.0 41.0 34.0 41.0 32-33 38.21572336461257 40.0 38.0 41.0 34.0 41.0 34-35 38.05387983697254 40.0 37.5 41.0 33.5 41.0 36-37 38.0361597645159 40.0 38.0 41.0 33.5 41.0 38-39 37.878002150060695 40.0 37.0 41.0 33.0 41.0 40-41 37.69093591384883 40.0 37.0 41.0 33.0 41.0 42-43 37.440880303106766 40.0 37.0 41.0 32.5 41.0 44-45 37.14254145407317 40.0 36.5 41.0 31.5 41.0 46-47 36.72817236873209 39.5 36.0 41.0 31.0 41.0 48-49 36.29800468657454 39.0 35.5 41.0 30.0 41.0 50-51 35.891396868799816 39.0 35.0 40.5 29.0 41.0 52-53 35.362137318360375 38.5 35.0 40.0 27.5 41.0 54-55 35.03211777657961 38.0 34.0 40.0 26.5 41.0 56-57 34.66796784736296 38.0 34.0 40.0 26.0 41.0 58-59 34.213905972361296 37.5 33.5 40.0 24.5 41.0 60-61 33.717315989864574 37.0 33.0 40.0 23.0 41.0 62-63 33.23859116576051 36.0 32.5 39.0 22.0 40.5 64-65 32.73611291869079 36.0 32.0 39.0 20.5 40.0 66-67 32.17563216963567 35.0 31.0 38.5 18.5 40.0 68-69 31.576149864143787 35.0 31.0 38.0 15.5 40.0 70-71 30.95460735828059 35.0 30.0 37.0 10.0 39.5 72-73 30.364095480490093 34.0 29.0 36.5 7.5 39.0 74-75 29.76484989415699 34.0 29.0 36.0 6.5 39.0 76-77 28.42501962146183 32.5 27.0 34.5 2.0 36.5 78-79 29.110033074281343 34.0 29.0 35.0 2.0 37.0 80-81 28.792034582909473 34.0 28.5 35.0 2.0 37.0 82-83 28.230180995535207 33.5 27.0 35.0 2.0 36.0 84-85 27.63797257510604 33.0 26.0 35.0 2.0 36.0 86-87 26.998161525467395 33.0 25.5 35.0 2.0 35.0 88-89 26.234363089341066 32.0 24.0 34.5 2.0 35.0 90-91 25.676129014265566 32.0 22.5 34.0 2.0 35.0 92-93 24.850821113356936 31.0 19.0 34.0 2.0 35.0 94-95 24.016214100360955 31.0 11.0 34.0 2.0 35.0 96-97 22.94902022172584 30.5 2.0 34.0 2.0 35.0 98-99 21.470499082339757 30.0 2.0 34.0 2.0 35.0 100-101 17.119228434984237 22.5 2.0 31.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.12148265898443356 1101 2 0.11782510492958664 1101 3 0.04269401941116513 1101 4 0.05630064058030371 1101 5 0.02922808439038249 1101 6 0.04227462646862534 1101 7 0.06568477330627331 1101 8 0.11926995779370486 1101 9 0.088470572767541 1101 10-11 0.11376857429095111 1101 12-13 0.1685090255554229 1101 14-15 0.14784179499186934 1101 16-17 0.14700383570040998 1101 18-19 0.1768626618672755 1101 20-21 0.19242466290236848 1101 22-23 0.1972217439299655 1101 24-25 0.2481864821104054 1101 26-27 0.1904542410603156 1101 28-29 0.21006820241717605 1101 30-31 0.1951548333798101 1101 32-33 0.26032907718814613 1101 34-35 0.17881210526982017 1101 36-37 0.2666985767862968 1101 38-39 0.20220240432670522 1101 40-41 0.09689196168775283 1101 42-43 0.1289966163847467 1101 44-45 0.17051842617691904 1101 46-47 0.15747821312466925 1101 48-49 0.19920973061609004 1101 50-51 0.13911172051872 1101 52-53 0.03555629747140898 1101 54-55 0.017800451605005208 1101 56-57 0.06819733416391216 1101 58-59 0.20660163498278195 1101 60-61 0.13163203277716207 1101 62-63 0.164969182674632 1101 64-65 0.15966277562926479 1101 66-67 -0.0368134161366811 1101 68-69 0.09760424131815526 1101 70-71 0.16353368079967368 1101 72-73 -0.009960478759719393 1101 74-75 -0.10351093029575154 1101 76-77 -0.14058521124530188 1101 78-79 -0.17598641676866578 1101 80-81 -0.15331624296198498 1101 82-83 -0.0848563273578371 1101 84-85 -0.14402115330660337 1101 86-87 -0.3621084750000385 1101 88-89 -0.42771617016806474 1101 90-91 -0.5151156406036037 1101 92-93 -0.6098013323297202 1101 94-95 -0.8576137442913634 1101 96-97 -0.5383427595246069 1101 98-99 -0.638846202915861 1101 100-101 -0.8586708775556389 1102 1 0.18632990066651445 1102 2 0.1876205429164699 1102 3 0.16884679641054845 1102 4 0.136265967786116 1102 5 0.09745024434106142 1102 6 0.10463050832705534 1102 7 0.13717738478789698 1102 8 0.08907802444916513 1102 9 0.15927092397903664 1102 10-11 0.14889308623774866 1102 12-13 0.19720494739218708 1102 14-15 0.2028350136210193 1102 16-17 0.3032468441631053 1102 18-19 0.2245157097478625 1102 20-21 0.24790995125074744 1102 22-23 0.17950686040888542 1102 24-25 0.2925991182515659 1102 26-27 0.1958085723573717 1102 28-29 0.15323852130973137 1102 30-31 0.08975305116829446 1102 32-33 0.10537538853338901 1102 34-35 0.12454365265535472 1102 36-37 0.1092086927981768 1102 38-39 0.10728069833556475 1102 40-41 0.05765894780742542 1102 42-43 0.08171973843074198 1102 44-45 0.100907112683295 1102 46-47 0.14395784098994113 1102 48-49 0.0381337531085677 1102 50-51 -0.013633072023637283 1102 52-53 -0.03257088592451396 1102 54-55 0.0366607329478299 1102 56-57 0.05519234621345959 1102 58-59 0.1846902972032609 1102 60-61 0.09337725499315752 1102 62-63 0.10909016382503722 1102 64-65 -0.03934030681202927 1102 66-67 0.028515732549074357 1102 68-69 0.056944785695971945 1102 70-71 -0.07398385175277511 1102 72-73 -0.43992733784545024 1102 74-75 -0.3251629693773701 1102 76-77 -0.22381688006033684 1102 78-79 -0.3803245512378304 1102 80-81 -0.26226974480210785 1102 82-83 -0.31430698027494586 1102 84-85 -0.3479699660996687 1102 86-87 -0.37616769523580373 1102 88-89 -0.451082345512269 1102 90-91 -0.777246448203222 1102 92-93 -0.5685318895977431 1102 94-95 -0.7910049154322429 1102 96-97 -0.6511963529038951 1102 98-99 -1.0209711818992169 1102 100-101 -1.4837325824258958 1103 1 0.1751652292113377 1103 2 0.1496636394847073 1103 3 0.06800295496335451 1103 4 0.056259583109344646 1103 5 0.08634516320245922 1103 6 0.09116735323262048 1103 7 0.09735615573762146 1103 8 0.023481855255226947 1103 9 0.11109002480372254 1103 10-11 0.17427749698528316 1103 12-13 0.19173966407215204 1103 14-15 0.1970164448527285 1103 16-17 0.2474681796246685 1103 18-19 0.24792087069492652 1103 20-21 0.24657661791741248 1103 22-23 0.23001497426894701 1103 24-25 0.19487525552211338 1103 26-27 0.19366651329910667 1103 28-29 0.22571950029350774 1103 30-31 0.16822924164448239 1103 32-33 0.12459220151556849 1103 34-35 0.048341471224119914 1103 36-37 0.007451842572052669 1103 38-39 -0.02447136338297895 1103 40-41 0.14887576078960052 1103 42-43 0.16628052587420683 1103 44-45 0.2979552637873084 1103 46-47 0.10252980161777003 1103 48-49 0.05788794305270528 1103 50-51 0.05045205700003663 1103 52-53 -0.10081243952920005 1103 54-55 -0.07438262964860343 1103 56-57 0.029979257626067124 1103 58-59 0.0015916007414347177 1103 60-61 -0.034784490151828606 1103 62-63 -0.05698911257825756 1103 64-65 0.06908108185516681 1103 66-67 -0.1452496305794142 1103 68-69 -0.07424436035351079 1103 70-71 -0.32511553571127294 1103 72-73 -0.34737187096596145 1103 74-75 -0.45334812500387045 1103 76-77 -0.4567064783577557 1103 78-79 -0.46376802024554564 1103 80-81 -0.409193926765397 1103 82-83 -0.6122202550820752 1103 84-85 -0.6929563986615186 1103 86-87 -0.786038405531091 1103 88-89 -0.8176883577495673 1103 90-91 -1.0654081933482082 1103 92-93 -1.0330043556791466 1103 94-95 -0.9199711298509712 1103 96-97 -1.2418747248113178 1103 98-99 -1.18326753731289 1103 100-101 -1.4512271288478171 1104 1 0.06813161807478707 1104 2 0.07155089624097855 1104 3 0.051499479436721174 1104 4 0.03582107079667196 1104 5 -0.002258135660973437 1104 6 -0.0093448505976923 1104 7 -0.03179101625747904 1104 8 0.01114529526340391 1104 9 0.07688678115583514 1104 10-11 0.112696500689772 1104 12-13 0.1449832455967126 1104 14-15 0.10299100588249388 1104 16-17 0.08244264907096266 1104 18-19 0.09998528175343324 1104 20-21 0.10528414939757624 1104 22-23 0.06922345410266217 1104 24-25 0.05953704039119856 1104 26-27 -0.05197895776515793 1104 28-29 -0.09998273925435797 1104 30-31 -0.031925189336746485 1104 32-33 0.03960313180461128 1104 34-35 0.13932972332025173 1104 36-37 -0.0035217827134772506 1104 38-39 0.131416326072042 1104 40-41 0.16919674097173498 1104 42-43 0.11613571779469112 1104 44-45 0.07397937280616418 1104 46-47 0.15799083152043636 1104 48-49 0.18950129884389355 1104 50-51 0.1509334011800405 1104 52-53 0.2225765871773433 1104 54-55 0.042695963576072415 1104 56-57 0.061653602704652144 1104 58-59 0.14769386099513326 1104 60-61 -0.03838020351412297 1104 62-63 0.011201489895022121 1104 64-65 -0.13411538063343187 1104 66-67 -0.2743908961038102 1104 68-69 -0.1328060882815585 1104 70-71 -0.31340373431734747 1104 72-73 -0.32854655516887377 1104 74-75 -0.3649174410876519 1104 76-77 -0.43771478590835144 1104 78-79 -0.35375217232190437 1104 80-81 -0.45207105491988386 1104 82-83 -0.5102049540047098 1104 84-85 -0.6058978077994261 1104 86-87 -0.7326242000044942 1104 88-89 -0.670754612423309 1104 90-91 -0.7920972780576143 1104 92-93 -0.7571435041651675 1104 94-95 -0.8965623264222984 1104 96-97 -0.8781613598617781 1104 98-99 -0.7584539229642822 1104 100-101 -1.4407611945835193 1105 1 0.0707122233797719 1105 2 0.12462636682877104 1105 3 0.09178204820911162 1105 4 0.06065651249321036 1105 5 0.06898022036024543 1105 6 0.011609610759400368 1105 7 0.05002150948813977 1105 8 0.042549761345362924 1105 9 0.09062001613388304 1105 10-11 0.06565225033503452 1105 12-13 0.07993739000951905 1105 14-15 0.07835676764636901 1105 16-17 0.06544211651665677 1105 18-19 0.04293982759644166 1105 20-21 0.103479666906793 1105 22-23 0.07051748048424855 1105 24-25 0.09574951222210615 1105 26-27 0.06424570836284715 1105 28-29 0.11274383134742294 1105 30-31 0.11968521971916601 1105 32-33 0.12218799559060045 1105 34-35 0.12135625971256303 1105 36-37 0.1328249997526143 1105 38-39 0.14729577199091892 1105 40-41 0.08686209957172508 1105 42-43 0.001083904240950062 1105 44-45 0.05964777729446524 1105 46-47 0.05055325037671565 1105 48-49 0.12600494187184097 1105 50-51 0.12149402629770378 1105 52-53 0.12812798355133026 1105 54-55 0.22504990732262087 1105 56-57 0.31202139725833433 1105 58-59 0.2481065356410852 1105 60-61 0.1662241203580095 1105 62-63 0.12513264412651637 1105 64-65 0.2354784667405525 1105 66-67 0.1341532796542495 1105 68-69 0.21215547428795034 1105 70-71 -0.04992218679902294 1105 72-73 -0.13809246288827381 1105 74-75 -0.03320270901423328 1105 76-77 0.07802895755528638 1105 78-79 0.09229483896429258 1105 80-81 0.0935685559858328 1105 82-83 0.06013769255821799 1105 84-85 -0.17382265537681718 1105 86-87 -0.1630001721114418 1105 88-89 -0.3809734040939752 1105 90-91 -0.4760816657434752 1105 92-93 -0.3153739587995261 1105 94-95 -0.28026288048877035 1105 96-97 -0.7266194430032442 1105 98-99 -0.9400708735744487 1105 100-101 -1.2816400849952707 1106 1 0.1764721194843375 1106 2 0.16321610017422472 1106 3 0.1737154291183245 1106 4 0.07711519541282996 1106 5 0.10343415855017923 1106 6 0.08018317014245468 1106 7 0.08896392889615612 1106 8 0.08610189780547728 1106 9 0.11370166571272478 1106 10-11 0.1349316410698691 1106 12-13 0.08026022634022212 1106 14-15 0.16687637708329106 1106 16-17 0.1850591766027847 1106 18-19 0.25781840347951857 1106 20-21 0.3411891546211052 1106 22-23 0.316302468732637 1106 24-25 0.28007160644255435 1106 26-27 0.24292666248886263 1106 28-29 0.27052078864026186 1106 30-31 0.2532030703180439 1106 32-33 0.27288872952662757 1106 34-35 0.24875812986714863 1106 36-37 0.14076135461357353 1106 38-39 0.23469394996328674 1106 40-41 0.24757443357277253 1106 42-43 0.13837338326668913 1106 44-45 0.256383686154507 1106 46-47 0.26091992448285595 1106 48-49 0.21329933462267547 1106 50-51 0.17022394316849443 1106 52-53 0.265853727888846 1106 54-55 -0.043748305734666815 1106 56-57 0.008044127140308888 1106 58-59 0.1168343033311885 1106 60-61 0.0024772166936557483 1106 62-63 0.14842324182836109 1106 64-65 0.15764322440206513 1106 66-67 0.006441431514993212 1106 68-69 -0.1366694106528108 1106 70-71 -0.4875224704173995 1106 72-73 -0.42020411558067394 1106 74-75 -0.18296662059657365 1106 76-77 -0.1670166757963223 1106 78-79 -0.18836336139996845 1106 80-81 -0.20424038185049298 1106 82-83 -0.07705075660585337 1106 84-85 -0.28733521568475595 1106 86-87 -0.3017068245604513 1106 88-89 -0.44937980497862995 1106 90-91 -0.7177290219297632 1106 92-93 -0.9910295277951136 1106 94-95 -1.028577748318746 1106 96-97 -1.3667102045571582 1106 98-99 -1.4090937403059591 1106 100-101 -1.791640663620841 1107 1 -0.01750382566114439 1107 2 0.026031362865751362 1107 3 0.026229187299016132 1107 4 0.0214686787054319 1107 5 -0.019817718465361622 1107 6 -0.03623455373638507 1107 7 -0.04410113696025775 1107 8 -0.035502420746666985 1107 9 -0.016262702987788202 1107 10-11 -0.014882032031295012 1107 12-13 -0.07905961935011163 1107 14-15 -0.05335202201541023 1107 16-17 -0.0984177907970718 1107 18-19 -0.025754640684425567 1107 20-21 -0.0165708958373898 1107 22-23 -0.12201410175985217 1107 24-25 -0.06697579536383813 1107 26-27 -0.012222867188242503 1107 28-29 -0.01545089453757953 1107 30-31 -0.013687087640271045 1107 32-33 -0.13852097960589305 1107 34-35 0.026223948068441416 1107 36-37 -0.01140402242182148 1107 38-39 -0.09230412170270341 1107 40-41 -0.05491010796810514 1107 42-43 -0.0986353504749573 1107 44-45 -0.09087358165610482 1107 46-47 -0.04740150411839039 1107 48-49 -0.20345536762225436 1107 50-51 -0.3659418914093848 1107 52-53 -0.2534052202811168 1107 54-55 -0.19207246098374497 1107 56-57 -0.29238642319526775 1107 58-59 -0.3364470431891391 1107 60-61 -0.47809046756768225 1107 62-63 -0.39543487658664134 1107 64-65 -0.696319974917202 1107 66-67 -0.7113584499485874 1107 68-69 -0.5101543230225722 1107 70-71 -0.6799119263772049 1107 72-73 -0.783243557388392 1107 74-75 -0.7158502200484271 1107 76-77 -0.9029761391548163 1107 78-79 -0.7894639085067432 1107 80-81 -0.6350195483049461 1107 82-83 -0.8086998262953209 1107 84-85 -0.73961380943582 1107 86-87 -0.7855763648387999 1107 88-89 -0.9280135492612871 1107 90-91 -1.2167490508637187 1107 92-93 -1.3197704593950164 1107 94-95 -1.568814172302229 1107 96-97 -1.6552203181424368 1107 98-99 -1.5904340105685257 1107 100-101 -2.1189479581432966 1108 1 -0.03839010998453318 1108 2 -0.06539325906284432 1108 3 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>>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 12.0 4 19.0 5 54.0 6 120.0 7 284.0 8 756.0 9 1345.0 10 2425.0 11 4101.0 12 6259.0 13 9309.0 14 13074.0 15 18065.0 16 23899.0 17 33520.0 18 33930.0 19 26944.0 20 25063.0 21 25727.0 22 27818.0 23 29362.0 24 33364.0 25 38119.0 26 42993.0 27 50948.0 28 61298.0 29 74384.0 30 88720.0 31 109227.0 32 136370.0 33 175973.0 34 238285.0 35 338774.0 36 474710.0 37 566693.0 38 286496.0 39 13565.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.761332591352016 18.96136340394109 15.56440827211766 22.712895732589228 2 15.81940039208811 19.997157626857504 39.58064370454195 24.602798276512434 3 19.322859373189367 23.793057993713234 29.795037591438522 27.089045041658878 4 13.343972982771604 16.977035887070535 36.23746847613748 33.441522654020375 5 14.00974908813954 36.930929682659944 33.891726821015055 15.167594408185462 6 32.388063120716424 36.10174561736394 16.696763517769096 14.813427744150538 7 28.8636019559045 32.81950114342326 19.99094955923092 18.325947341441324 8 26.92038002555106 34.23569924117068 19.237299502524564 19.606621230753703 9 26.307665849493095 16.86539345181022 20.04838632566713 36.77855437302956 10-11 25.235772946154196 26.424116403409286 26.435769758911665 21.904340891524857 12-13 26.34436742432354 23.711866283464037 27.061768432662397 22.881997859550026 14-15 22.97327895543438 25.07513260256945 25.724616269279498 26.22697217271667 16-17 22.181198282694105 28.248858621075478 26.709329276572774 22.860613819657647 18-19 22.262374469124914 26.364637809726933 29.363418025450134 22.00956969569802 20-21 22.680766452147537 25.48158902632397 29.900385390742656 21.93725913078584 22-23 22.55078671769796 25.36947113022276 29.832125279879733 22.247616872199544 24-25 22.5082804434121 25.566483261817396 29.76741383225827 22.157822462512236 26-27 21.912606593523364 25.99160202428074 29.611083069893475 22.48470831230242 28-29 22.125025047276477 26.38026472169757 29.412832074591826 22.081878156434122 30-31 22.169205002333232 25.898610335718363 29.30750524292771 22.624679419020694 32-33 21.907395332283315 25.84513387746646 29.396850871578028 22.850619918672194 34-35 22.72214809579757 25.950441048076257 29.10713102845807 22.2202798276681 36-37 22.847538377898317 25.48405330367407 29.094725658722027 22.573682659705593 38-39 22.2163769552017 25.957179997499946 29.06236143659878 22.76408161069957 40-41 22.766983465703216 25.741433751506293 29.132657016555548 22.358925766234947 42-43 22.485277236179275 25.776917860465964 28.828976889307228 22.90882801404753 44-45 22.41491390461114 26.06357619236071 29.04892682887961 22.47258307414854 46-47 22.961823449222877 26.405790692316017 28.149479117903482 22.48290674055762 48-49 22.65773170856007 26.492539233619507 28.00237185421795 22.84735720360247 50-51 22.816858667008425 26.711111671852233 28.00133271932181 22.47069694181754 52-53 23.182337810328622 26.396895908614443 27.89171605904441 22.529050222012525 54-55 22.668266604864957 26.648251218630293 28.005553520954194 22.67792865555055 56-57 22.7425949123825 26.74111393636618 27.798849587128128 22.717441564123188 58-59 22.708070264575568 26.371514935663864 27.80641128138457 23.114003518376 60-61 22.342216475395045 26.713206449011395 28.05010187079367 22.89447520479989 62-63 22.42537195722305 26.68138279590959 28.253789306427002 22.639455940440357 64-65 22.1879542637596 26.747448651332146 27.981809110222084 23.082787974686173 66-67 22.179340206151334 26.74201364878898 27.912645094131154 23.16600105092853 68-69 22.683997750975312 26.39548627520397 28.111994920100177 22.808521053720536 70-71 22.602525594561033 26.596328077253794 27.552769299709436 23.24837702847574 72-73 22.507534789519774 26.662571747548235 27.586385500666115 23.243507962265873 74-75 22.431543551843973 27.145988677189663 27.355134754526063 23.067333016440305 76-77 22.654724554827364 26.669546817842505 27.70304733719951 22.972681290130623 78-79 22.611069212716433 26.897939163530925 27.650333598671384 22.840658025081254 80-81 22.797903289188937 26.978624314520566 27.32220451148441 22.901267884806085 82-83 22.811783144286295 26.985724576624413 27.328735936052695 22.8737563430366 84-85 22.656564435594955 27.198317829755975 27.32837586356675 22.816741871082318 86-87 22.931444003924163 27.11118181280774 27.021313904684224 22.936060278583874 88-89 22.76733605214107 27.29273629369234 26.7869364398717 23.152991214294893 90-91 22.704897127186094 27.23341637901776 26.980891965114175 23.080794528681977 92-93 22.78498541499519 26.836408847704924 27.14575905784588 23.232846679454006 94-95 22.62837309025489 26.642112662634027 27.273987554095125 23.455526693015955 96-97 22.862932414214466 26.39066536502434 27.251044302326356 23.49535791843484 98-99 22.58233642412956 26.7352130461252 26.86412765484819 23.818322874897046 100-101 23.095360079370163 26.002793760755928 26.898796066422044 24.00305009345186 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1278.0 1 849.5 2 627.5 3 800.0 4 650.0 5 625.5 6 1241.0 7 2917.0 8 4887.0 9 5621.5 10 4514.0 11 3283.5 12 3165.0 13 3343.0 14 3621.0 15 3878.0 16 4080.5 17 4376.0 18 4778.5 19 5220.0 20 5906.0 21 6964.0 22 8281.5 23 10095.5 24 12399.0 25 15144.5 26 18827.0 27 23348.0 28 26795.5 29 30128.0 30 34413.5 31 39854.0 32 47788.5 33 51468.5 34 51246.5 35 54110.5 36 60374.5 37 66400.0 38 70581.5 39 74400.0 40 78625.0 41 83661.0 42 86569.5 43 89417.0 44 93619.5 45 99371.0 46 103207.5 47 106961.0 48 109893.0 49 108550.5 50 107285.0 51 105103.5 52 104081.0 53 107516.5 54 112177.5 55 108126.5 56 99301.5 57 91148.5 58 85088.5 59 77165.5 60 62608.0 61 51880.0 62 44798.5 63 33609.0 64 23076.5 65 17304.5 66 13180.0 67 10221.0 68 7950.5 69 6157.5 70 4746.5 71 3368.0 72 2367.5 73 1859.0 74 1414.5 75 1014.0 76 724.0 77 452.5 78 302.5 79 193.0 80 112.5 81 62.5 82 35.0 83 21.0 84 14.5 85 9.5 86 7.0 87 3.5 88 1.0 89 1.0 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.008366511642731361 2 0.01460819493175317 3 0.00946212626261285 4 1.3280177210684698E-4 5 0.0011288150629081995 6 0.0021580287967362635 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 8.134108541544378E-4 14-15 0.0 16-17 0.0026892358851636515 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0030544407584574807 26-27 0.018857851639172273 28-29 0.0067562901559358405 30-31 0.0031042414229975487 32-33 0.0 34-35 0.008681915851485122 36-37 6.806090820475908E-4 38-39 0.0024734330054900252 40-41 0.0018758250310092137 42-43 0.0030046400939174136 44-45 0.0 46-47 6.640088605342349E-5 48-49 6.142081959941673E-4 50-51 0.004017253606232122 52-53 0.00800130676943753 54-55 0.007470099681010143 56-57 0.015952812874334994 58-59 0.022426899264543786 60-61 0.003469446296291378 62-63 0.01902385385430583 64-65 0.020268870467807523 66-67 0.01279877078679738 68-69 0.03071040979970837 70-71 0.015919612431308284 72-73 0.00483066446038656 74-75 0.015820011102228148 76-77 0.019654662271813353 78-79 0.025066334485167367 80-81 0.011238349964541927 82-83 0.03416325587448639 84-85 0.021912292397629755 86-87 0.030610808470628234 88-89 0.021215083094068807 90-91 0.027025160623743362 92-93 0.02310750834659138 94-95 0.013778183856085374 96-97 0.014209789615432627 98-99 0.010773543762167962 100-101 0.009860531578933388 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3012008.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.92268777394423 #Duplication Level Percentage of deduplicated Percentage of total 1 71.30086286263628 32.743272632536616 2 14.86206246287392 13.650117083188313 3 5.538020576708741 7.629623694896223 4 2.606294175366406 4.787521346496037 5 1.439800016450774 3.3059743306194336 6 0.9073868683102133 2.5001786306152187 7 0.6040405429124935 1.9417415678481944 8 0.44385179575682127 1.6306293947555979 9 0.30914266785856653 1.2776995992305786 >10 1.7809633821303885 15.030964508857064 >50 0.13415922273930292 4.269120519473935 >100 0.06575883595009177 5.590990985924811 >500 0.0035393672169939036 1.1270465988312388 >1k 0.003900527136968025 3.345547454515167 >5k 7.223198401792638E-5 0.2641396258135624 >10k+ 1.4446396803585276E-4 0.9054320263979655 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14958 0.4966122267935543 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12338 0.4096270660635695 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7963 0.26437512782170564 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 4332 0.14382431919171532 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 3722 0.12357204894542113 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 3710 0.12317364362910059 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 3660 0.121513621477765 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 3430 0.11387751958162129 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3211 0.10660662255877142 No Hit GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 3162 0.10497980085046255 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 3.3200443026711746E-5 0.0 0.0 0.0 6.640088605342349E-5 2 6.640088605342349E-5 0.0 0.0 0.0 6.640088605342349E-5 3 6.640088605342349E-5 0.0 0.0 0.0 6.640088605342349E-5 4 6.640088605342349E-5 0.0 0.0 0.0 6.640088605342349E-5 5 6.640088605342349E-5 0.0 0.0 0.0 9.960132908013525E-5 6 6.640088605342349E-5 0.0 0.0 0.0 9.960132908013525E-5 7 6.640088605342349E-5 0.0 0.0 0.0 9.960132908013525E-5 8 6.640088605342349E-5 0.0 0.0 0.0 9.960132908013525E-5 9 6.640088605342349E-5 0.0 0.0 0.0 9.960132908013525E-5 10-11 6.640088605342349E-5 0.0 0.0 0.0 9.960132908013525E-5 12-13 9.960132908013525E-5 0.0 0.0 0.0 3.4860465178047337E-4 14-15 1.3280177210684698E-4 0.0 0.0 0.0 3.9840531632054095E-4 16-17 1.3280177210684698E-4 0.0 0.0 1.6600221513355873E-5 4.8140642388732035E-4 18-19 1.6600221513355875E-4 0.0 0.0 4.980066454006762E-5 5.644075314540998E-4 20-21 1.992026581602705E-4 0.0 0.0 6.640088605342349E-5 5.976079744808115E-4 22-23 1.992026581602705E-4 0.0 0.0 1.4940199362020287E-4 5.976079744808115E-4 24-25 2.6560354421369396E-4 0.0 0.0 3.8180509480718514E-4 5.976079744808115E-4 26-27 2.6560354421369396E-4 0.0 0.0 0.0011122148413948436 6.972093035609467E-4 28-29 3.8180509480718514E-4 0.0 0.0 0.00341964563175131 7.470099681010144E-4 30-31 3.98405316320541E-4 0.0 0.0 0.010009933572553592 7.636101896143702E-4 32-33 3.98405316320541E-4 0.0 0.0 0.023837918093179034 7.96810632641082E-4 34-35 4.1500553783389686E-4 0.0 0.0 0.04473759697849408 8.632115186945054E-4 36-37 4.648062023739645E-4 0.0 0.0 0.07782183845461234 8.632115186945054E-4 38-39 4.648062023739645E-4 0.0 0.0 0.1297141309053628 8.798117402078612E-4 40-41 4.648062023739645E-4 0.0 0.0 0.18210443000151394 8.964119617212172E-4 42-43 4.980066454006762E-4 0.0 0.0 0.2387775862481109 9.130121832345731E-4 44-45 5.644075314540998E-4 0.0 0.0 0.3091459252432265 0.0010126135123147083 46-47 5.644075314540998E-4 0.0 0.0 0.3870341645838922 0.0011122148413948436 48-49 5.644075314540998E-4 0.0 0.0 0.47026767525185853 0.0012284163919883348 50-51 5.644075314540998E-4 0.0 0.0 0.5625151062015772 0.0012948172780417582 52-53 5.644075314540998E-4 0.0 0.0 0.6469936334830452 0.001427619050148605 54-55 5.644075314540998E-4 0.0 0.0 0.7396560699705976 0.0014940199362020287 56-57 5.976079744808115E-4 0.0 0.0 0.8544632019569669 0.0015438206007420963 58-59 5.976079744808115E-4 0.0 0.0 0.9697683405887367 0.001593621265282164 60-61 5.976079744808115E-4 0.0 0.0 1.096743434944396 0.001593621265282164 62-63 5.976079744808115E-4 0.0 0.0 1.219800877022903 0.0016932225943622993 64-65 5.976079744808115E-4 0.0 0.0 1.3431903235316773 0.0016932225943622993 66-67 6.308084175075232E-4 0.0 0.0 1.4894880757288824 0.001709822815875655 68-69 6.308084175075232E-4 0.0 0.0 1.648617799155912 0.0017264230373890109 70-71 6.64008860534235E-4 0.0 0.0 1.81654563998502 0.0017596234804157225 72-73 6.64008860534235E-4 0.0 0.0 1.9900843556856422 0.0018260243664691461 74-75 6.64008860534235E-4 0.0 0.0 2.16760712454947 0.0018758250310092137 76-77 6.64008860534235E-4 0.0 0.0 2.3607673020788793 0.0019422259170626373 78-79 6.64008860534235E-4 0.0 0.0 2.57486035893663 0.001992026581602705 80-81 7.636101896143702E-4 0.0 0.0 2.797386328323165 0.002008626803116061 82-83 7.636101896143702E-4 0.0 0.0 3.029573626630474 0.0020584274676561285 84-85 7.636101896143702E-4 0.0 0.0 3.270426240567754 0.0020584274676561285 86-87 7.636101896143702E-4 0.0 0.0 3.52011017235014 0.002141428575222908 88-89 7.636101896143702E-4 0.0 0.0 3.774624768592912 0.0022742303473297547 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7445 0.0 51.80622 1 GTATCAA 12785 0.0 40.162018 1 TATCAAC 16265 0.0 31.509644 2 ATCAACG 16335 0.0 31.28427 3 TCAACGC 16510 0.0 30.863806 4 CAACGCA 16825 0.0 30.285969 5 AACGCAG 17340 0.0 29.468636 6 TAAGGTG 1980 0.0 28.541622 5 GTAAGGT 2100 0.0 26.910671 4 ACGCAGA 19690 0.0 25.830534 7 CGCAGAG 19650 0.0 25.810614 8 GGTAAGG 2155 0.0 24.683323 3 AGGTAAG 2160 0.0 24.628637 2 GTGGTAT 3940 0.0 23.870392 1 TGGTATC 3740 0.0 23.368046 2 GAGTACT 13265 0.0 23.307312 12-13 GCAGAGT 21550 0.0 23.292566 9 GGACCGA 890 0.0 22.944324 6 AAGGTAA 2335 0.0 22.376717 1 GTACTTT 13905 0.0 21.927166 14-15 >>END_MODULE