##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938287_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 43149 Sequences flagged as poor quality 0 Sequence length 101 %GC 34 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.0155044149343 33.0 31.0 34.0 30.0 34.0 2 32.20556675705115 33.0 31.0 34.0 30.0 34.0 3 32.13179911469559 34.0 31.0 34.0 30.0 34.0 4 35.772416510231984 37.0 35.0 37.0 35.0 37.0 5 35.74840668381654 37.0 35.0 37.0 35.0 37.0 6 35.88138774942641 37.0 35.0 37.0 35.0 37.0 7 35.8507729031959 37.0 35.0 37.0 35.0 37.0 8 35.90377529027324 37.0 35.0 37.0 35.0 37.0 9 37.36821247305847 39.0 37.0 39.0 34.0 39.0 10-11 37.426637929036595 39.0 37.0 39.0 34.0 39.0 12-13 37.33779461864701 39.0 37.0 39.0 34.0 39.0 14-15 38.123606572574104 40.0 37.0 41.0 33.0 41.0 16-17 38.03775290273239 40.0 37.5 41.0 33.0 41.0 18-19 38.40398386984634 40.0 37.0 41.0 34.0 41.0 20-21 38.51584045980208 40.0 37.5 41.0 34.0 41.0 22-23 38.320818558946904 40.0 37.0 41.0 34.0 41.0 24-25 38.02187767966812 40.0 36.0 41.0 33.5 41.0 26-27 37.559711696678946 39.5 35.0 41.0 33.0 41.0 28-29 37.18516072214884 39.0 35.0 41.0 33.0 41.0 30-31 36.42996361445225 37.5 35.0 40.0 32.5 41.0 32-33 35.44339382140953 36.0 35.0 40.0 31.0 41.0 34-35 34.635727363322445 35.0 35.0 40.0 30.0 41.0 36-37 34.04033697188811 35.0 34.5 40.0 25.5 41.0 38-39 33.24366729240539 35.0 33.0 39.0 22.0 41.0 40-41 32.789021761802125 35.0 33.0 39.0 18.0 41.0 42-43 32.00128624070082 35.0 33.0 39.0 2.0 41.0 44-45 31.054114811467244 35.0 33.0 39.0 2.0 41.0 46-47 29.82779438689193 35.0 30.5 39.0 2.0 41.0 48-49 28.698301235254583 35.0 25.5 39.0 2.0 41.0 50-51 27.58974715520638 35.0 19.0 38.0 2.0 40.5 52-53 26.746042781987995 35.0 3.5 38.0 2.0 40.0 54-55 25.956151938631255 35.0 2.0 38.0 2.0 40.0 56-57 24.867239101717303 34.0 2.0 37.5 2.0 40.0 58-59 23.66652761361793 33.0 2.0 36.0 2.0 40.0 60-61 22.30226656469443 32.0 2.0 35.0 2.0 39.0 62-63 21.066907691951144 30.5 2.0 35.0 2.0 39.0 64-65 19.95048552689518 28.5 2.0 35.0 2.0 38.5 66-67 18.620141834109713 22.5 2.0 35.0 2.0 37.5 68-69 17.46093768105866 2.0 2.0 35.0 2.0 37.0 70-71 16.62132378502399 2.0 2.0 35.0 2.0 36.0 72-73 16.051009293378755 2.0 2.0 34.0 2.0 35.5 74-75 15.628438666017752 2.0 2.0 34.0 2.0 35.0 76-77 14.85244153978076 2.0 2.0 32.0 2.0 34.5 78-79 15.106340819022456 2.0 2.0 33.0 2.0 35.0 80-81 14.882500173816311 2.0 2.0 33.0 2.0 35.0 82-83 14.589364759322347 2.0 2.0 33.0 2.0 35.0 84-85 14.255822846415908 2.0 2.0 33.0 2.0 35.0 86-87 13.94881689031032 2.0 2.0 32.5 2.0 35.0 88-89 13.590280191893207 2.0 2.0 32.0 2.0 35.0 90-91 13.259832209321189 2.0 2.0 31.5 2.0 35.0 92-93 12.822904354677977 2.0 2.0 31.0 2.0 35.0 94-95 12.426661104544717 2.0 2.0 31.0 2.0 35.0 96-97 11.9216088437739 2.0 2.0 30.0 2.0 34.5 98-99 11.284096966325986 2.0 2.0 29.0 2.0 34.0 100-101 9.50623421168509 2.0 2.0 21.5 2.0 31.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.10563311455244317 1101 2 0.5226957536189261 1101 3 0.020510972785736215 1101 4 -0.23272979743922662 1101 5 0.7229376727332735 1101 6 0.6222813849965547 1101 7 0.7106917611384844 1101 8 0.3387823121920661 1101 9 -0.14767981382994577 1101 10-11 0.02354925201314728 1101 12-13 0.3547612665856903 1101 14-15 0.3954155864236526 1101 16-17 0.6158932046204626 1101 18-19 1.1435250830723547 1101 20-21 0.7461241092504096 1101 22-23 0.6948242353831446 1101 24-25 0.6580483242998199 1101 26-27 1.0570501839797615 1101 28-29 0.8257716900711216 1101 30-31 1.4878759422813346 1101 32-33 1.245126543889448 1101 34-35 1.6906488601829608 1101 36-37 1.4962067346881085 1101 38-39 1.672104141220892 1101 40-41 1.7927296413745921 1101 42-43 3.3910875275077608 1101 44-45 3.124457089756401 1101 46-47 0.9139475545836184 1101 48-49 1.6041204016718993 1101 50-51 1.8555566950293105 1101 52-53 1.4706755775373779 1101 54-55 1.9824755989550127 1101 56-57 1.5799571147651434 1101 58-59 2.243531969609645 1101 60-61 1.0643458743478718 1101 62-63 0.4079690160685949 1101 64-65 -0.12401595448767111 1101 66-67 0.26904255686167744 1101 68-69 1.2507895632185253 1101 70-71 0.2639692349055949 1101 72-73 -0.6342276487616836 1101 74-75 -1.2842523005018336 1101 76-77 -0.7167842829258912 1101 78-79 -0.9658317114244976 1101 80-81 -1.5878112853789776 1101 82-83 -1.1716230834241657 1101 84-85 -1.9038551076824302 1101 86-87 -1.577018749569083 1101 88-89 -0.952547556492199 1101 90-91 -1.2010523342932427 1101 92-93 -0.5795909196712614 1101 94-95 0.09855655636766691 1101 96-97 0.06751521572181574 1101 98-99 -0.7785337942735371 1101 100-101 -0.9950277181832661 1102 1 -0.022299781219111026 1102 2 0.4785781065601 1102 3 0.12181816232821774 1102 4 -0.6657363333869384 1102 5 -0.8947093860902555 1102 6 -0.43490815748710787 1102 7 0.30382901604044577 1102 8 0.17865159323781654 1102 9 0.5451306436863916 1102 10-11 -0.03609127086267705 1102 12-13 0.26244100514777813 1102 14-15 -0.13236219135412597 1102 16-17 -0.10551202413770966 1102 18-19 -0.0239585770583588 1102 20-21 -0.21792817833129163 1102 22-23 0.3484190066249724 1102 24-25 0.27651237658740513 1102 26-27 0.17388024933923418 1102 28-29 0.11090240902536408 1102 30-31 1.06303934097415 1102 32-33 0.04251216480447795 1102 34-35 -0.9915406823007018 1102 36-37 -2.5111462064883625 1102 38-39 -2.957797819563421 1102 40-41 -2.028348789997956 1102 42-43 -2.621984367917076 1102 44-45 -4.938451406975627 1102 46-47 -4.641608000971939 1102 48-49 -6.757807702903264 1102 50-51 -6.334802782094876 1102 52-53 -6.202527036841708 1102 54-55 -5.724223747450214 1102 56-57 -5.344062493077992 1102 58-59 -4.836533389867476 1102 60-61 -5.022255432841671 1102 62-63 -4.1892532061536265 1102 64-65 -5.005551902200086 1102 66-67 -3.6860228026154473 1102 68-69 -3.6470862537749387 1102 70-71 -2.794037301042117 1102 72-73 -2.2812864722910966 1102 74-75 -2.0726509933122905 1102 76-77 -2.0084509495925573 1102 78-79 -2.431517985934301 1102 80-81 -1.636013899758062 1102 82-83 -1.6332243906137087 1102 84-85 -1.074606741669358 1102 86-87 -1.3948292070854222 1102 88-89 -0.7728089944006964 1102 90-91 -0.2631438375612163 1102 92-93 0.282337184903902 1102 94-95 0.47682453022387605 1102 96-97 -0.6432690980036746 1102 98-99 -0.5620305262996812 1102 100-101 0.7966389484834 1103 1 0.4527002187808904 1103 2 0.27269575361892606 1103 3 0.6232887505635176 1103 4 0.3283813136718905 1103 5 0.0729376727332749 1103 6 0.419503607218779 1103 7 0.5023584278051487 1103 8 0.536004534414289 1103 9 0.6245424083922728 1103 10-11 0.5249381409020302 1103 12-13 0.8242057110301317 1103 14-15 0.36763780864587403 1103 16-17 0.6297820935093483 1103 18-19 0.34074730529458463 1103 20-21 0.3475129981392939 1103 22-23 0.3087131242720318 1103 24-25 -0.0183405645890673 1103 26-27 0.5584390728686444 1103 28-29 -0.22145053215110977 1103 30-31 -0.3274018354964454 1103 32-33 -0.3229290116661119 1103 34-35 0.3378710824051794 1103 36-37 0.4962067346881085 1103 38-39 -0.6695625254457696 1103 40-41 -1.136437025292075 1103 42-43 -0.5116902502700178 1103 44-45 -1.0935984657991575 1103 46-47 -2.0791080009719387 1103 48-49 -1.7181018205503236 1103 50-51 -0.7833321938595788 1103 52-53 -0.39737997801817926 1103 54-55 0.23386448784389913 1103 56-57 1.0132904480984806 1103 58-59 1.3796430807207578 1103 60-61 1.3740680965700918 1103 62-63 0.9357467938463735 1103 64-65 1.8370951566234446 1103 66-67 2.3676536679727853 1103 68-69 3.1896784521074153 1103 70-71 2.337580346016704 1103 72-73 2.7907723512383136 1103 74-75 2.631025477275948 1103 76-77 2.72904905040744 1103 78-79 2.5508349552421716 1103 80-81 3.4566331590654684 1103 82-83 3.838099138798057 1103 84-85 3.84892267009535 1103 86-87 4.113259028208697 1103 88-89 4.491896887952246 1103 90-91 4.367003221262312 1103 92-93 4.655131302550959 1103 94-95 3.81800100081211 1103 96-97 3.538348549055147 1103 98-99 4.090910650170908 1103 100-101 4.0216389484834 1104 1 0.22770021878088897 1104 2 0.43936242028559036 1104 3 0.3816220838968505 1104 4 0.10060353589410909 1104 5 0.22293767273327347 1104 6 0.12228138499655472 1104 7 -0.09486379441707271 1104 8 0.6721156455254018 1104 9 -0.34212425827438864 1104 10-11 0.46799369645759015 1104 12-13 0.6464279332523546 1104 14-15 0.08986003086809546 1104 16-17 0.5186709823982341 1104 18-19 0.3101917497390261 1104 20-21 0.5100129981392953 1104 22-23 0.7920464576053661 1104 24-25 1.2552705465220484 1104 26-27 0.8070501839797615 1104 28-29 0.2007716900711145 1104 30-31 -0.10934627994088686 1104 32-33 0.3284598772227767 1104 34-35 0.38509330462740365 1104 36-37 0.7323178457992228 1104 38-39 1.2693263634431133 1104 40-41 1.2649518635968136 1104 42-43 1.4605319719522107 1104 44-45 1.9855682008675082 1104 46-47 1.4972808879169506 1104 48-49 2.4652315127830136 1104 50-51 2.4944455839182 1104 52-53 0.33178668864848504 1104 54-55 -0.25363551215609803 1104 56-57 -0.2533762185681887 1104 58-59 0.09075419183186995 1104 60-61 -0.24120968120768538 1104 62-63 -0.41147542837584794 1104 64-65 0.2648729344012217 1104 66-67 1.0051536679727882 1104 68-69 -0.34643265900369613 1104 70-71 0.4584136793500413 1104 72-73 -0.6620054265394621 1104 74-75 -0.6036967449462765 1104 76-77 0.13043793929633196 1104 78-79 -0.14638726698005122 1104 80-81 0.45385538128768843 1104 82-83 0.7311546943536129 1104 84-85 1.4850337812064591 1104 86-87 -0.24368541623574735 1104 88-89 0.8946746657300242 1104 90-91 0.18783655459564486 1104 92-93 -0.30181314189348285 1104 94-95 -2.05422122141011 1104 96-97 -2.1269292287226307 1104 98-99 -1.7229782387179817 1104 100-101 -2.4811388292943777 1105 1 0.3290515701322434 1105 2 0.529452510375684 1105 3 0.014504966779732342 1105 4 0.5067596920502666 1105 5 0.22293767273327347 1105 6 0.12153063424580068 1105 7 0.47870977915650315 1105 8 0.6285721019818524 1105 9 0.8718397056895739 1105 10-11 0.2941948976587909 1105 12-13 0.5660976029220279 1105 14-15 0.3406107816188424 1105 16-17 0.5689712826985414 1105 18-19 0.6934500079972921 1105 20-21 1.0066346197609164 1105 22-23 0.7729023134612234 1105 24-25 -0.2139486726971711 1105 26-27 0.4020201539497279 1105 28-29 0.2690900083894334 1105 30-31 -0.4453072409018475 1105 32-33 -0.23610468734178625 1105 34-35 0.8148981094322068 1105 36-37 0.776612140093512 1105 38-39 1.792599636716389 1105 40-41 0.805117028761984 1105 42-43 0.01871515513538924 1105 44-45 -1.1371795468802368 1105 46-47 0.2705541611902227 1105 48-49 -0.5403991178476204 1105 50-51 -2.6177916533190384 1105 52-53 -3.470015113153316 1105 54-55 -2.6793111878317752 1105 56-57 -4.2995473897393595 1105 58-59 -5.3480596219819425 1105 60-61 -6.186404876402879 1105 62-63 -5.101415368315788 1105 64-65 -5.671688627160341 1105 66-67 -6.170522007702887 1105 68-69 -4.593429656000694 1105 70-71 -4.182352086415726 1105 72-73 -4.458551973086006 1105 74-75 -3.753096144345676 1105 76-77 -3.096288787430396 1105 78-79 -2.711327206919991 1105 80-81 -2.525123597691291 1105 82-83 -2.6960224828235653 1105 84-85 -3.1024286812560025 1105 86-87 -2.991808539358873 1105 88-89 -2.994589598534242 1105 90-91 -2.0238751571160662 1105 92-93 -1.7729092379895803 1105 94-95 -2.2509179181068077 1105 96-97 -3.551854153647554 1105 98-99 -4.046927187666931 1105 100-101 -3.669577267732816 1106 1 0.0027002187808875533 1106 2 0.04769575361892464 1106 3 0.12328875056351762 1106 4 0.2033813136718905 1106 5 -0.502062327266728 1106 6 0.1445036072187733 1106 7 -0.6476415721948499 1106 8 -0.3639954655857096 1106 9 -0.5754575916077229 1106 10-11 0.32493814090203443 1106 12-13 0.02420571103013458 1106 14-15 -0.31986219135412597 1106 16-17 -0.1952179064906545 1106 18-19 0.5657473052945861 1106 20-21 -0.08998700186070607 1106 22-23 0.2462131242720318 1106 24-25 0.531659435410937 1106 26-27 0.6584390728686529 1106 28-29 0.4785494678488931 1106 30-31 -0.2524018354964426 1106 32-33 0.014570988333893808 1106 34-35 0.23787108240518506 1106 36-37 -0.4037932653118901 1106 38-39 0.6429374745542304 1106 40-41 1.3135629747079278 1106 42-43 -0.5741902502700178 1106 44-45 0.08140153420084673 1106 46-47 -0.5416080009719373 1106 48-49 -1.6806018205503221 1106 50-51 -2.5833321938595795 1106 52-53 -1.2223799780181785 1106 54-55 -1.8036355121560952 1106 56-57 -2.636709551901518 1106 58-59 -1.7953569192792393 1106 60-61 -1.7759319034299068 1106 62-63 -1.3767532061536265 1106 64-65 -1.6754048433765583 1106 66-67 -1.8573463320272126 1106 68-69 -0.8728215478925847 1106 70-71 -0.8499196539832958 1106 72-73 -0.671727648761685 1106 74-75 -0.6189745227240557 1106 76-77 0.0040490504074419675 1106 78-79 0.3508349552421688 1106 80-81 0.169133159065467 1106 82-83 0.5130991387980561 1106 84-85 0.3239226700953477 1106 86-87 0.5507590282086952 1106 88-89 0.7293968879522463 1106 90-91 1.254503221262313 1106 92-93 1.1301313025509607 1106 94-95 0.9805010008121116 1106 96-97 1.0633485490551493 1106 98-99 1.3534106501709076 1106 100-101 0.6216389484833993 1107 1 0.23351417226925975 1107 2 0.42385854431660164 1107 3 0.07735851800537574 1107 4 0.2214045694858413 1107 5 0.4089841843611808 1107 6 0.5363640723350542 1107 7 0.45293982315398296 1107 8 0.47960918557707544 1107 9 -0.21615526602632684 1107 10-11 0.2467404664834234 1107 12-13 0.6044382691696697 1107 14-15 1.1118238551575033 1107 16-17 0.7635030237419045 1107 18-19 1.223305444829471 1107 20-21 1.0710595097672027 1107 22-23 1.3511549847371427 1107 24-25 1.3941594354109341 1107 26-27 0.6697762821709716 1107 28-29 0.9494797004070321 1107 30-31 0.6926563040384437 1107 32-33 0.9628268022873812 1107 34-35 0.3925222451958774 1107 36-37 1.38283464166485 1107 38-39 2.1536932885077107 1107 40-41 2.530132742149789 1107 42-43 1.8342399822881212 1107 44-45 1.7578550225729366 1107 46-47 1.7711826967024784 1107 48-49 0.23364236549618767 1107 50-51 0.4114352480008847 1107 52-53 0.8860502545399598 1107 54-55 2.1358993715648324 1107 56-57 1.1429416108891743 1107 58-59 1.401154708627736 1107 60-61 0.7516843756398615 1107 62-63 0.5026072589626516 1107 64-65 0.7400021333676285 1107 66-67 0.6912001796006955 1107 68-69 -0.5667168967297975 1107 70-71 -0.2615475609600395 1107 72-73 -0.9365532301570312 1107 74-75 -0.36403266225893915 1107 76-77 -0.3747300193599994 1107 78-79 -0.5375371377810847 1107 80-81 -0.23348312000430127 1107 82-83 0.2976921620538704 1107 84-85 0.03962034451395269 1107 86-87 -0.16842701830293194 1107 88-89 0.5732922367894542 1107 90-91 0.2963636863785908 1107 92-93 0.4420499072021222 1107 94-95 0.04009402406792617 1107 96-97 -0.13984912536345817 1107 98-99 0.5118408827290466 1107 100-101 1.5233831345299116 1108 1 0.44281649785065724 1108 2 0.5401376140840455 1108 3 0.3796840994007269 1108 4 0.29117201134630477 1108 5 0.3159609285472271 1108 6 0.0014803514048224997 1108 7 0.4064281952470097 1108 8 -0.14829779116710995 1108 9 0.13268194327599758 1108 10-11 -0.26488744049331814 1108 12-13 -0.5350966145512572 1108 14-15 0.07694013422727153 1108 16-17 0.20536348885818256 1108 18-19 -0.23018292726355583 1108 20-21 0.1873385795346465 1108 22-23 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2311 12-13 -0.5757942889698668 2311 14-15 -0.21569552468745457 2311 16-17 -0.536884573157316 2311 18-19 -0.4050860280387454 2311 20-21 -0.8337370018607047 2311 22-23 -0.926703542394641 2311 24-25 0.18582610207760553 2311 26-27 -0.4186442604646885 2311 28-29 0.18688280118222167 2311 30-31 -1.0919851688297726 2311 32-33 -1.341679011666109 2311 34-35 -1.253795584261482 2311 36-37 -1.1600432653118915 2311 38-39 -0.827895858779101 2311 40-41 -0.4468536919587436 2311 42-43 -0.0741902502700178 2311 44-45 -0.18109846579915612 2311 46-47 -0.7249413343052744 2311 48-49 0.04856484611634215 2311 50-51 0.37291780614042125 2311 52-53 0.23803668864848504 2311 54-55 0.15261448784390197 2311 56-57 1.1112071147651434 2311 58-59 1.562976414054095 2311 60-61 1.807401429903429 2311 62-63 1.3315801271797056 2311 64-65 2.0287618232901075 2311 66-67 1.5780703346394525 2311 68-69 1.8584284521074146 2311 70-71 1.4375803460167056 2311 72-73 0.6678556845716521 2311 74-75 0.743525477275945 2311 76-77 1.0019657170741088 2311 78-79 1.1383349552421702 2311 80-81 1.054549825732133 2311 82-83 0.9672658054647236 2311 84-85 1.1343393367620145 2311 86-87 0.9924256948753634 2311 88-89 1.0439802212855778 2311 90-91 1.1045032212623127 2311 92-93 0.5280479692176279 2311 94-95 0.32425100081211156 2311 96-97 1.2133485490551479 2311 98-99 1.6763273168375754 2311 100-101 1.7341389484834 2312 1 -0.24678957713747707 2312 2 -0.6966920014831146 2312 3 -0.6710990045385259 2312 4 -0.6063125638791362 2312 5 -0.14440926604223847 2312 6 -0.2932514948220373 2312 7 0.37225638698882335 2312 8 -0.4287913839530617 2312 9 0.0031138369637062624 2312 10-11 -0.6768475733836752 2312 12-13 -0.4380391869290534 2312 14-15 -0.4282805587010685 2312 16-17 -0.3140954575110584 2312 18-19 0.18462485631498993 2312 20-21 -0.07417067533009458 2312 22-23 0.0428967977414203 2312 24-25 -0.14665689111968305 2312 26-27 0.08624519531762331 2312 28-29 -0.057164817865391626 2312 30-31 -1.039901835496444 2312 32-33 7.954781298096236E-4 2312 34-35 1.0327690415888569 2312 36-37 -0.3124667346996475 2312 38-39 -0.3695625254457724 2312 40-41 -0.6060798824349334 2312 42-43 -0.4887310665965465 2312 44-45 0.5357382688947219 2312 46-47 0.5267593459668376 2312 48-49 1.4507757304700846 2312 50-51 1.7906473979771533 2312 52-53 1.3385894097369224 2312 54-55 1.5779971409051292 2312 56-57 1.1301271827923571 2312 58-59 1.5179083868432066 2312 60-61 1.6028946271823372 2312 62-63 1.8770733244586175 2312 64-65 1.1733196464193618 2312 66-67 1.0357659128707475 2312 68-69 0.11671926843394687 2312 70-71 1.097019121526909 2312 72-73 1.3963845961362757 2312 74-75 1.235872416051457 2312 76-77 1.9864470095911173 2312 78-79 2.0834880164666583 2312 80-81 1.864286220289955 2312 82-83 1.7215175061449948 2312 84-85 1.8611675680545314 2312 86-87 1.8002488241270633 2312 88-89 1.910264234891022 2312 90-91 2.589197098813333 2312 92-93 1.696202731122387 2312 94-95 1.7394295722406845 2312 96-97 1.7120730388510665 2312 98-99 1.8419310583341737 2312 100-101 2.8323532341976847 2313 1 0.09090776595070338 2313 2 -0.4159834916640932 2313 3 -0.31491879660629607 2313 4 -0.32444887500735575 2313 5 0.0342584274502542 2313 6 0.22657907891689177 2313 7 0.14622635233344994 2313 8 0.0044007608293838985 2313 9 0.1783159932979359 2313 10-11 -0.09463733079608261 2313 12-13 0.3911868431056078 2313 14-15 -0.27387162531639575 2313 16-17 -0.24285941592461313 2313 18-19 -0.03755458149786506 2313 20-21 0.20576771512042313 2313 22-23 0.1874867091776906 2313 24-25 0.1488764165430112 2313 26-27 0.3492409596610955 2313 28-29 -0.11107317366054303 2313 30-31 0.45066420223940185 2313 32-33 1.1282502336169102 2313 34-35 1.693531459763669 2313 36-37 2.1259237158201856 2313 38-39 1.7342110594598878 2313 40-41 0.9869120313116966 2313 42-43 1.103875787465828 2313 44-45 0.9627694587291451 2313 46-47 1.2687693575186252 2313 48-49 1.7852000662421297 2313 50-51 -0.8375774768784439 2313 52-53 0.43870492764219904 2313 54-55 -0.1262770215900595 2313 56-57 -0.6881246462411426 2313 58-59 -0.12648899475093955 2313 60-61 -0.13796020531670194 2313 62-63 0.40980339761995666 2313 64-65 0.23473666605740462 2313 66-67 0.5240215925010894 2313 68-69 -0.22117060449635773 2313 70-71 -1.0235045596436727 2313 72-73 -0.9927182147994191 2313 74-75 -0.6597764095165068 2313 76-77 -1.522601892988785 2313 78-79 -1.105768818342737 2313 80-81 -0.2778008031986836 2313 82-83 0.10484442181692444 2313 84-85 0.12628116066138695 2313 86-87 0.5002873300954871 2313 88-89 0.6051044351220565 2313 90-91 0.4299749193755211 2313 92-93 0.4374426233056763 2313 94-95 0.43215194420833924 2313 96-97 0.9244334547155262 2313 98-99 0.9795898954539268 2313 100-101 2.461733288106041 2314 1 -0.30531864914363993 2314 2 -0.1895683973244715 2314 3 -0.6168055890591262 2314 4 -0.6829394410450931 2314 5 -0.9468736480214446 2314 6 -0.45266620410198044 2314 7 -0.3254717608740947 2314 8 -0.5427690504913727 2314 9 -0.08583495009828823 2314 10-11 -0.7833165760790948 2314 12-13 -1.2314546663283537 2314 14-15 -0.43424898380695964 2314 16-17 0.14393303690557957 2314 18-19 -0.5847243928186217 2314 20-21 -1.2376285112946661 2314 22-23 -1.2370415927090974 2314 24-25 -1.4737650928909503 2314 26-27 -0.9809477195841865 2314 28-29 -1.0167335510190298 2314 30-31 -0.5304678732322898 2314 32-33 -0.5038252380812054 2314 34-35 0.3822107050466883 2314 36-37 0.47498031959376874 2314 38-39 0.8474186066297023 2314 40-41 -0.06025778000905291 2314 42-43 -0.3678223257417166 2314 44-45 0.3212600247668824 2314 46-47 1.1178259612922083 2314 48-49 1.3229359152987357 2314 50-51 0.8416678061404212 2314 52-53 0.41040304084974366 2314 54-55 1.1850437331269212 2314 56-57 -0.15038879718454012 2314 58-59 1.5150204392113231 2314 60-61 1.2771341343059426 2314 62-63 1.4569732089407132 2314 64-65 1.8762461000196708 2314 66-67 1.4957197057086375 2314 68-69 1.2976973200319435 2314 70-71 1.2312124214884044 2314 72-73 1.4412440493515248 2314 74-75 2.028902835766509 2314 76-77 0.44909622021876316 2314 78-79 1.2715896722233015 2314 80-81 0.8071048571786754 2314 82-83 1.7180519689867353 2314 84-85 2.5979792738689316 2314 86-87 2.2550043112275606 2314 88-89 1.614538397386207 2314 90-91 1.6563900137151428 2314 92-93 1.4280086610415275 2314 94-95 1.809510434774376 2314 96-97 2.424433454715526 2314 98-99 1.1588351784727937 2314 100-101 1.5277710239550988 2315 1 -0.28155904047837055 2315 2 -0.41248943156626083 2315 3 -0.49800754573278283 2315 4 -0.2605075752169981 2315 5 -0.9437289939333908 2315 6 -0.23882972611455955 2315 7 -1.261530461083737 2315 8 -0.7908473174375672 2315 9 -0.6291612953114267 2315 10-11 -1.0921914887276003 2315 12-13 -0.649868363043943 2315 14-15 -0.4379177469096831 2315 16-17 -0.26836605463880403 2315 18-19 -0.45369713914985965 2315 20-21 -1.0686907055644141 2315 22-23 -0.9070276164687101 2315 24-25 -0.9947294534779516 2315 26-27 -1.4198016678720933 2315 28-29 -0.8825616432622212 2315 30-31 -1.5861981317927416 2315 32-33 -1.4678364190735138 2315 34-35 -2.147314102780001 2315 36-37 -2.1102747467933725 2315 38-39 -2.1010440069272533 2315 40-41 -2.832270358625408 2315 42-43 -3.3033569169366856 2315 44-45 -2.8709132806139728 2315 46-47 -3.345311704675641 2315 48-49 -4.409768487216986 2315 50-51 -4.2879618234892085 2315 52-53 -4.603398496536698 2315 54-55 -3.83696884548943 2315 56-57 -3.4894873296792994 2315 58-59 -2.580542104464424 2315 60-61 -2.0467652367632425 2315 62-63 -2.0874013543017718 2315 64-65 -0.6008678063395188 2315 66-67 -0.5318833690642499 2315 68-69 0.4220858595148229 2315 70-71 1.0463766423130032 2315 72-73 0.3657723512383164 2315 74-75 0.43334029209076164 2315 76-77 0.1026601615185534 2315 78-79 -0.31768356327634883 2315 80-81 0.5557072331395414 2315 82-83 0.7496732128721302 2315 84-85 -0.16774399657131767 2315 86-87 0.48316643561610384 2315 88-89 0.22337836943372658 2315 90-91 0.5582069249660169 2315 92-93 1.2583720432917005 2315 94-95 1.7976306304417413 2315 96-97 1.5767744749810735 2315 98-99 1.3649847242449802 2315 100-101 1.6392315410759917 2316 1 -0.5607613196806511 2316 2 0.13808036900353926 2316 3 -0.20171124943648522 2316 4 0.28222746751804095 2316 5 0.20370690350250698 2316 6 -0.34780408508891725 2316 7 0.011973812420535523 2316 8 0.04177376518352105 2316 9 -0.02161143776157104 2316 10-11 -0.010638782174886785 2316 12-13 -0.5757942889698668 2316 14-15 -0.5265929605848996 2316 16-17 -0.13944867572142527 2316 18-19 -0.018868079320803588 2316 20-21 -0.009217771091478255 2316 22-23 0.17025158581049027 2316 24-25 -0.269302103050606 2316 26-27 -0.1790609271313528 2316 28-29 -0.16568130138188053 2316 30-31 -0.5975941431887506 2316 32-33 0.08380175756466457 2316 34-35 -0.7409750714409711 2316 36-37 -0.2153317268503514 2316 38-39 -0.8214856023688526 2316 40-41 -1.3450908714459189 2316 42-43 -0.0213056348854046 2316 44-45 1.1362092265085373 2316 46-47 0.9353150759511379 2316 48-49 0.9299751025266012 2316 50-51 0.6108985753711913 2316 52-53 -1.6137261318643326 2316 54-55 -1.2055585890791747 2316 56-57 -1.0963249365169077 2316 58-59 -2.2607415346638575 2316 60-61 -2.0980472880452936 2316 62-63 -2.3527147446151666 2316 64-65 -2.2148279202996335 2316 66-67 -1.0429232551041352 2316 68-69 -1.7353215478925854 2316 70-71 -2.0479965770602178 2316 72-73 -2.6534584179924536 2316 74-75 -1.9295514458009784 2316 76-77 -1.7872971034387106 2316 78-79 -1.8953188909116765 2316 80-81 -1.787597610165303 2316 82-83 -1.9782470150480975 2316 84-85 -1.7222311760584983 2316 86-87 -1.8088563564066895 2316 88-89 -0.8542569582016011 2316 90-91 -1.2897275479684573 2316 92-93 -1.7900610051413466 2316 94-95 -0.6973836145725052 2316 96-97 -0.5606899124833138 2316 98-99 -0.18024319598293914 2316 100-101 -0.2033610515166 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 1.0 5 4.0 6 19.0 7 28.0 8 57.0 9 153.0 10 259.0 11 448.0 12 606.0 13 904.0 14 1092.0 15 1467.0 16 1765.0 17 2163.0 18 2135.0 19 1689.0 20 1740.0 21 1716.0 22 1840.0 23 1653.0 24 1747.0 25 1753.0 26 1334.0 27 736.0 28 725.0 29 782.0 30 866.0 31 947.0 32 1096.0 33 1413.0 34 2123.0 35 3599.0 36 2754.0 37 2704.0 38 816.0 39 15.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.92576884748198 23.55326890542075 14.760481123548633 14.760481123548633 2 15.307588892494554 26.17866580130731 38.66997357563395 19.843771730564182 3 21.627885417632335 34.68063409659776 34.75711504588857 8.934365439881338 4 10.069758279450276 28.772393334723862 28.735312521727042 32.42253586409882 5 6.22578924172485 44.06995894319316 33.65034445965067 16.053907355431328 6 18.598345268720017 41.25472200978007 12.283019305198266 27.86391341630165 7 11.979420148786762 46.459941134209366 18.584439963846208 22.97619875315766 8 24.23694639504971 32.9880182623004 18.155693063570418 24.61934227907947 9 19.972652900414843 20.922848733458483 19.669053744003335 39.43544462212334 10-11 21.702704581797956 31.706412663097638 20.585645090268603 26.0052376648358 12-13 28.863936591809775 30.656562145125033 23.382928920716587 17.09657234234861 14-15 24.022572944911815 31.092261697837724 27.063199610651463 17.821965746598995 16-17 14.76975132679784 37.62080233609122 27.437484066838163 20.171962270272775 18-19 13.451064914598252 31.37732044775082 35.9614359544833 19.210178683167626 20-21 15.634197779786321 29.43637164244826 35.69839393728708 19.23103664047834 22-23 14.083756286356577 34.5396185311363 36.39713550719599 14.97948967531113 24-25 15.81149041692739 37.05300238707734 35.61612088345037 11.519386312544903 26-27 14.729535552053397 36.89858162603134 37.538240474645406 10.833642347269862 28-29 12.591253563234375 38.2616051356926 36.70884609145055 12.43829520962247 30-31 16.163758140397228 36.316021228765436 36.01937472479084 11.500845906046491 32-33 12.787086606873856 37.034461980578925 38.74481448005748 11.433636932489746 34-35 11.966624174295978 34.35044616989222 38.85965928844593 14.82327036736586 36-37 9.709379128137384 37.80388885026304 39.93140049595588 12.555331525643702 38-39 11.226606408250769 32.4607451184889 40.99310504664233 15.319543426617996 40-41 10.562180579216355 33.21976149914821 41.34478322845322 14.873274693182214 42-43 12.892272748342837 32.02614379084967 40.919204561257125 14.162378899550365 44-45 10.973080087608498 32.58201708133916 40.988260924988126 15.456641906064222 46-47 14.245984843217688 30.380773598461147 38.85142181742335 16.52181974089782 48-49 13.331711047764722 28.975179030800252 39.11678138543188 18.57632853600315 50-51 9.921435027462977 29.37379777051612 42.39379823402628 18.310968967994622 52-53 11.641057730190735 30.26026095622146 41.654499524902086 16.444181788685714 54-55 10.9214582029711 29.52907367494032 38.47366103501819 21.075807087070384 56-57 14.693272149991888 30.42712461470718 34.6265266865976 20.25307654870333 58-59 12.534473568332984 33.24642517787203 37.479431736540825 16.739669517254168 60-61 13.11965514843913 37.41917541542099 32.36459709379128 17.09657234234861 62-63 10.026883589422699 39.64750052144893 31.563883288141092 18.761732600987276 64-65 10.38610396532944 40.07161232010012 30.867459269044474 18.674824445525967 66-67 11.42552550464669 37.56981621822059 29.95318547359151 21.05147280354122 68-69 11.26213817237943 35.69491761106862 30.925398039352014 22.117546177199934 70-71 13.2969477855802 34.20241488794642 29.83151405594568 22.669123270527706 72-73 13.020000463510161 31.380796773969273 32.21627384180398 23.382928920716587 74-75 12.908758024519686 31.33676330853554 30.51635032098079 25.238128345963982 76-77 12.604000092702034 29.590488771466315 32.6890542075135 25.116456928318154 78-79 13.379220839416906 29.074833715729216 32.74931052863334 24.79663491622054 80-81 14.014229761987531 28.683167628450253 31.784050615309738 25.518551994252476 82-83 13.239315843144524 28.756836933345692 32.48238620561787 25.521461017891905 84-85 13.346929788984555 30.023059897794823 30.86318180238015 25.76682851084047 86-87 13.629516327145472 30.536049502885348 29.782845488887343 26.05158868108183 88-89 13.539131845465713 30.968272729379592 27.808292196806416 27.684303228348284 90-91 14.420959929546456 29.664650397459962 29.06440473707386 26.849984935919718 92-93 14.75468724651788 30.257943405409165 28.445618670189344 26.541750677883613 94-95 14.628380727247444 29.64379244014925 27.88708892442467 27.840737908178635 96-97 13.867065285406383 30.510556443950033 28.113050128624067 27.509328142019513 98-99 14.506709309601613 30.583559294537533 26.34939396046258 28.56033743539827 100-101 14.38387911654963 29.424783888386752 26.91140003244571 29.279936962617903 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1345.0 1 767.0 2 241.0 3 306.0 4 217.0 5 154.5 6 259.0 7 445.0 8 604.0 9 622.0 10 444.0 11 241.5 12 204.5 13 214.0 14 221.5 15 237.0 16 266.5 17 278.5 18 301.5 19 357.0 20 406.0 21 461.5 22 533.5 23 581.5 24 644.0 25 753.5 26 845.5 27 935.0 28 994.5 29 1038.0 30 1082.5 31 1118.0 32 1116.5 33 1073.0 34 1065.0 35 971.5 36 801.0 37 676.0 38 616.5 39 615.5 40 616.0 41 660.5 42 745.0 43 831.5 44 898.5 45 942.0 46 1034.5 47 1451.0 48 2252.5 49 2488.0 50 2290.0 51 1980.0 52 1172.5 53 533.0 54 307.0 55 237.0 56 227.0 57 210.0 58 167.5 59 124.5 60 90.0 61 63.5 62 56.5 63 45.0 64 43.0 65 48.5 66 46.5 67 47.0 68 37.5 69 29.0 70 25.0 71 15.0 72 11.0 73 12.0 74 9.0 75 5.0 76 5.0 77 2.5 78 2.0 79 1.5 80 0.0 81 1.0 82 1.5 83 0.5 84 0.0 85 0.5 86 0.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.01622285568611092 3 0.00231755081230156 4 0.0 5 0.08806693086745927 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.00231755081230156 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.00927020324920624 36-37 0.0 38-39 0.003476326218452339 40-41 0.010428978655357019 42-43 0.006952652436904678 44-45 0.0057938770307539 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.00231755081230156 84-85 0.00115877540615078 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 43149.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 38.08663004936383 #Duplication Level Percentage of deduplicated Percentage of total 1 84.33734939759037 32.12125425849962 2 7.64877692588536 5.826322742126122 3 2.799075088231715 3.1982201209761523 4 1.3569429232079833 2.067255324572991 5 0.7849580138736765 1.494820273934506 6 0.6024096385542169 1.3766251825071265 7 0.30424729219909946 0.811142784305546 8 0.23122794207131558 0.7045354469396742 9 0.13995375441158572 0.4797330181464229 >10 1.4421321650237313 10.171730515191546 >50 0.14603870025556773 4.213307376764235 >100 0.1582085919435317 12.973649447264131 >500 0.012169891687963978 2.7184871028297293 >1k 0.03650967506389193 21.8429164059422 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT 2021 4.683770191661452 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1746 4.046443718278523 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1735 4.020950659343206 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1590 3.68490579155948 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1199 2.77874342394957 No Hit ATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTT 1134 2.628102621149969 RNA PCR Primer, Index 33 (95% over 22bp) ACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCT 648 1.5017729263714106 RNA PCR Primer, Index 33 (95% over 24bp) GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 525 1.2167141764583187 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 447 1.0359452130987972 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 433 1.0034995017265753 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 428 0.9919117476650675 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 390 0.9038448167976084 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 308 0.7138056501888804 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 287 0.6651370831305476 No Hit CATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCT 260 0.6025632111984055 RNA PCR Primer, Index 33 (96% over 27bp) TTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTC 255 0.5909754571368977 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 255 0.5909754571368977 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 208 0.48205056895872445 No Hit CACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTG 205 0.47509791652181976 RNA PCR Primer, Index 15 (95% over 24bp) TATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCT 176 0.4078889429650745 RNA PCR Primer, Index 33 (95% over 21bp) GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 167 0.3870309856543605 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 165 0.3823958840297573 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 163 0.37776078240515426 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 160 0.3708081299682496 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 153 0.35458527428213865 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 151 0.34995017265753553 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 143 0.33140976615912304 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 138 0.31982201209761524 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 128 0.2966465039745997 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 126 0.2920114023499965 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 119 0.2757885466638856 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 119 0.2757885466638856 No Hit CTTTACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTC 113 0.2618832417900762 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 101 0.23407263204245754 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 98 0.22711997960555286 No Hit GGTATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 97 0.2248024287932513 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 96 0.2224848779809497 No Hit CTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTC 95 0.22016732716864817 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 93 0.21553222554404503 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 89 0.20626202229483884 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 86 0.19930936985793415 No Hit ACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGC 84 0.194674268233331 RNA PCR Primer, Index 15 (96% over 25bp) AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 82 0.1900391666087279 No Hit CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 81 0.18772161579642632 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 80 0.1854040649841248 No Hit GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACAAGAGGC 80 0.1854040649841248 No Hit TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 78 0.18076896335952167 No Hit GGTATCAACGCAGAGTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 75 0.17381631092261698 No Hit GTACCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT 73 0.16918120929801386 No Hit AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 67 0.1552759044242045 No Hit GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC 63 0.14600570117499825 No Hit TCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCTTG 61 0.14137059955039513 RNA PCR Primer, Index 33 (96% over 29bp) GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 59 0.13673549792579204 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 59 0.13673549792579204 No Hit CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATATCGTATGCCGT 58 0.13441794711349045 No Hit GTATCAACGCAGAGTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 56 0.12978284548888736 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 54 0.12514774386428423 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 54 0.12514774386428423 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 50 0.11587754061507798 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 50 0.11587754061507798 No Hit GTATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 49 0.11355998980277643 No Hit GGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 49 0.11355998980277643 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 48 0.11124243899047485 No Hit TCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTGCTTG 45 0.10428978655357019 RNA PCR Primer, Index 23 (96% over 30bp) >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.006952652436904679 0.0 22-23 0.0 0.0 0.0 0.028969385153769497 0.0 24-25 0.0 0.0 0.0 0.06489142274444368 0.0 26-27 0.0 0.0 0.0 0.18424528957797398 0.0 28-29 0.0 0.0 0.0 0.3997775151220191 0.0 30-31 0.0 0.0 0.0 0.7949199286194351 0.0 32-33 0.0 0.0 0.0 1.2781292729843101 0.0 34-35 0.0 0.0 0.0 1.9594892118009688 0.0 36-37 0.0 0.0 0.0 2.7659968944819115 0.0 38-39 0.0 0.0 0.0 3.564393149319799 0.0 40-41 0.0 0.0 0.0 4.360471853345384 0.0 42-43 0.0 0.0 0.0 5.121787295186447 0.0 44-45 0.0 0.0 0.0 5.679158265544972 0.0 46-47 0.0 0.0 0.0 6.236529235903497 0.0 48-49 0.0 0.0 0.0 6.726691232705278 0.0 50-51 0.0 0.0 0.0 7.22032955572551 0.0 52-53 0.0 0.0 0.0 7.5679621775707435 0.0 54-55 0.0 0.0 0.0 7.980486222160421 0.0 56-57 0.0 0.0 0.0 8.489188625460613 0.0 58-59 0.0 0.0 0.0 8.849567776773506 0.0 60-61 0.0 0.0 0.0 9.172866115089573 0.0 62-63 0.0 0.0 0.0 9.441702009316554 0.0 64-65 0.0 0.0 0.0 9.65607545945445 0.0 66-67 0.0 0.0 0.0 9.930705230712183 0.0 68-69 0.0 0.0 0.0 10.3281651950219 0.0 70-71 0.0 0.0 0.0 10.651463533337969 0.0 72-73 0.0 0.0 0.0 10.929569630814155 0.0 74-75 0.0 0.0 0.0 11.243597765881017 0.0 76-77 0.0 0.0 0.0 11.489258151984982 0.0 78-79 0.0 0.0 0.0 11.829938121393312 0.0 80-81 0.0 0.0 0.0 12.148601358084775 0.0 82-83 0.0 0.0 0.0 12.396579295001043 0.0 84-85 0.0 0.0 0.0 12.619064172981993 0.0 86-87 0.0 0.0 0.0 12.846184152587547 0.0 88-89 0.0 0.0 0.0 13.097638415722265 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCCAT 35 3.482637E-8 81.518166 2 GTACCCA 40 8.7882654E-8 71.3284 1 ACCCATG 40 8.7882654E-8 71.3284 3 ATGGGAT 25 0.0049669268 57.062717 5 GTGCAAA 30 0.00393206 31.664732 14-15 GAGTGCA 30 0.00393206 31.664732 12-13 CTTATAC 880 0.0 21.614666 1 GAGTACT 1090 0.0 21.351906 12-13 GGTATCA 1785 0.0 21.31194 1 GTACAAA 135 3.6872132E-4 21.13434 1 AGTACTT 900 0.0 21.109821 12-13 GTACTTT 990 0.0 20.630054 14-15 TACAAAA 140 4.713136E-4 20.379541 2 TATACAC 950 0.0 20.022007 3 TTATACA 975 0.0 19.508621 2 ACTTTTT 1055 0.0 19.359007 16-17 GTATCAA 2170 0.0 18.40733 1 TACTTTT 1035 0.0 18.356369 14-15 AGTAAAA 80 0.0011794062 17.811413 12-13 TACACAT 1090 0.0 17.45037 5 >>END_MODULE