##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938285_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 43721 Sequences flagged as poor quality 0 Sequence length 101 %GC 34 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.01951007524988 33.0 31.0 34.0 30.0 34.0 2 32.21868209784772 33.0 31.0 34.0 30.0 34.0 3 32.164360376020674 34.0 31.0 34.0 30.0 34.0 4 35.77397589259166 37.0 35.0 37.0 35.0 37.0 5 35.78349077102536 37.0 35.0 37.0 35.0 37.0 6 35.917293748999334 37.0 35.0 37.0 35.0 37.0 7 35.85046087692413 37.0 35.0 37.0 35.0 37.0 8 35.9174309828229 37.0 35.0 37.0 35.0 37.0 9 37.41220466137554 39.0 37.0 39.0 34.0 39.0 10-11 37.46442213124128 39.0 37.0 39.0 34.5 39.0 12-13 37.39437570046431 39.0 37.0 39.0 34.0 39.0 14-15 38.21765284417099 40.0 37.5 41.0 33.0 41.0 16-17 38.148292582511836 40.0 37.5 41.0 33.0 41.0 18-19 38.40175201848082 40.0 37.5 41.0 34.0 41.0 20-21 38.52712655245763 40.0 37.5 41.0 35.0 41.0 22-23 38.29377187164063 40.0 37.0 41.0 34.0 41.0 24-25 38.05381853114064 40.0 36.0 41.0 33.5 41.0 26-27 37.619210448068436 40.0 35.5 41.0 33.0 41.0 28-29 37.27900779945564 39.0 35.0 41.0 33.0 41.0 30-31 36.50603828823678 37.5 35.0 40.5 32.5 41.0 32-33 35.569257336291486 36.0 35.0 40.0 31.0 41.0 34-35 34.875666155851874 35.0 35.0 40.0 30.0 41.0 36-37 34.260732828617826 35.0 35.0 40.0 28.0 41.0 38-39 33.34248987900551 35.0 33.5 39.0 22.5 41.0 40-41 32.94686763797718 35.0 33.0 39.5 18.5 41.0 42-43 32.2147594977242 35.0 33.0 40.0 2.0 41.0 44-45 31.280208595411814 35.0 33.0 40.0 2.0 41.0 46-47 30.040941424029644 35.0 31.5 40.0 2.0 41.0 48-49 28.943654079275404 35.0 27.0 39.0 2.0 41.0 50-51 27.8543034239839 35.0 20.0 39.0 2.0 41.0 52-53 26.94152695501018 35.0 2.0 38.5 2.0 40.0 54-55 26.26873813499234 35.0 2.0 38.5 2.0 40.0 56-57 25.30914205987969 35.0 2.0 37.5 2.0 40.0 58-59 24.218544864024153 34.0 2.0 37.0 2.0 40.0 60-61 22.956588367146225 33.0 2.0 36.0 2.0 40.0 62-63 21.840980306946317 32.0 2.0 35.0 2.0 39.5 64-65 20.64892157086983 30.5 2.0 35.0 2.0 39.0 66-67 19.191612726149906 25.0 2.0 35.0 2.0 38.5 68-69 17.93831339630841 5.0 2.0 35.0 2.0 37.5 70-71 16.997564099631756 2.0 2.0 35.0 2.0 37.0 72-73 16.493527137988607 2.0 2.0 35.0 2.0 36.0 74-75 16.13673063287665 2.0 2.0 35.0 2.0 35.5 76-77 15.368518560874637 2.0 2.0 33.0 2.0 35.0 78-79 15.599860478946045 2.0 2.0 34.0 2.0 35.0 80-81 15.410100409414241 2.0 2.0 34.0 2.0 35.0 82-83 15.149847899178884 2.0 2.0 34.0 2.0 35.0 84-85 14.91350838269939 2.0 2.0 34.0 2.0 35.0 86-87 14.707314562795911 2.0 2.0 34.0 2.0 35.0 88-89 14.414594817135931 2.0 2.0 33.0 2.0 35.0 90-91 14.23147915189497 2.0 2.0 33.0 2.0 35.0 92-93 14.048352050502046 2.0 2.0 33.0 2.0 35.0 94-95 13.869604995311178 2.0 2.0 33.0 2.0 35.0 96-97 13.570080739232864 2.0 2.0 33.0 2.0 35.0 98-99 13.266267926168203 2.0 2.0 33.0 2.0 35.0 100-101 12.301777178015142 2.0 2.0 30.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.13291041732365017 1101 2 0.012894185786549883 1101 3 -0.8271483328970852 1101 4 -0.12280974866007455 1101 5 0.023504619148532413 1101 6 -0.02359063344441381 1101 7 -0.1277964654553756 1101 8 -0.47446543750827175 1101 9 -1.0191101485319365 1101 10-11 -0.8053462622689906 1101 12-13 0.2249083601241253 1101 14-15 -0.17551061563201387 1101 16-17 -0.9951228757586534 1101 18-19 -0.17331485086733522 1101 20-21 -0.4876479636675981 1101 22-23 0.09191745043329291 1101 24-25 0.5886992406814926 1101 26-27 0.4274160780413325 1101 28-29 -0.2920326997416822 1101 30-31 0.46742240445827576 1101 32-33 0.8291004219950366 1101 34-35 1.455777938817178 1101 36-37 0.669481178199355 1101 38-39 0.8812681769895434 1101 40-41 1.2008548352882826 1101 42-43 0.8978074635842788 1101 44-45 0.9063464173784617 1101 46-47 -1.5059270486530743 1101 48-49 -2.0995332773630366 1101 50-51 -2.054987681023132 1101 52-53 -2.2886727325991068 1101 54-55 -1.255326210371038 1101 56-57 -0.9806659438373622 1101 58-59 0.3232599444568933 1101 60-61 0.5299014820351324 1101 62-63 1.5450303116356778 1101 64-65 0.7014043985116913 1101 66-67 -0.866251808179193 1101 68-69 -0.33203938966440916 1101 70-71 0.07914635729039432 1101 72-73 -0.1551811155023728 1101 74-75 -0.18938484827125812 1101 76-77 -0.7279954898352496 1101 78-79 -0.5485814246802043 1101 80-81 -1.6698801216759271 1101 82-83 -1.5798234867927583 1101 84-85 -1.4655491857688023 1101 86-87 -1.4697972020905556 1101 88-89 -1.1288301024351686 1101 90-91 -2.3016333718030975 1101 92-93 -2.698894912587523 1101 94-95 -1.7574452121016897 1101 96-97 -1.1632218019202956 1101 98-99 -1.0323807522901003 1101 100-101 -1.6784453096622975 1102 1 0.2099666943799292 1102 2 0.399041371933734 1102 3 0.18237547662672426 1102 4 0.06593484008451611 1102 5 0.1464483420922491 1102 6 0.17987257001879442 1102 7 0.14319920554029864 1102 8 0.013846250803418059 1102 9 -0.12906685848864186 1102 10-11 0.595086638163906 1102 12-13 0.23140186661763096 1102 14-15 0.208472068350666 1102 16-17 0.19362171298593722 1102 18-19 0.4236548461023659 1102 20-21 -0.43829731431695507 1102 22-23 0.2096663681822193 1102 24-25 0.4302576822399331 1102 26-27 0.51962387024912 1102 28-29 -0.12579893350791593 1102 30-31 0.1791107161465888 1102 32-33 -0.3397307468361319 1102 34-35 -0.004395221355984802 1102 36-37 0.012338321056496682 1102 38-39 0.9418742375956057 1102 40-41 1.185270419703862 1102 42-43 1.793911359688174 1102 44-45 2.4132728243048724 1102 46-47 1.5551119123858896 1102 48-49 1.3610727832430243 1102 50-51 1.0857049596695099 1102 52-53 2.1117601678337934 1102 54-55 2.399219244174418 1102 56-57 3.3262604630890458 1102 58-59 2.3535629747599245 1102 60-61 2.9199447720784235 1102 62-63 2.7714372380426013 1102 64-65 2.7918805889878797 1102 66-67 3.2411074991801136 1102 68-69 2.290038532413515 1102 70-71 3.8280641062081457 1102 72-73 3.9387582784370245 1102 74-75 3.2101822512958424 1102 76-77 2.873736111896351 1102 78-79 2.728041951943169 1102 80-81 2.1946220428262393 1102 82-83 2.440522833553562 1102 84-85 2.9063122860926693 1102 86-87 3.128471196177843 1102 88-89 3.32917855557349 1102 90-91 2.7433882732185477 1102 92-93 2.305001191308582 1102 94-95 2.291472536816059 1102 96-97 1.7566916179931233 1102 98-99 0.6416452217358746 1102 100-101 -0.10268773390472319 1103 1 0.36601065042388825 1103 2 0.10380327669564338 1103 3 0.22413371838496943 1103 4 0.4109897851394635 1103 5 0.27758387322778333 1103 6 0.293425683571904 1103 7 0.5761662385073265 1103 8 0.4556044925616618 1103 9 0.2745961451743568 1103 10-11 0.24710129017856275 1103 12-13 -0.0011988659831061454 1103 14-15 -0.744275184396578 1103 16-17 -0.1151694958052687 1103 18-19 0.04929587174338934 1103 20-21 -0.04708852310815814 1103 22-23 -0.19363033511448435 1103 24-25 0.14930530128755493 1103 26-27 -0.3884347378094901 1103 28-29 0.3350069272979397 1103 30-31 0.06299350002937132 1103 32-33 0.2207088136034301 1103 34-35 0.47326045629969116 1103 36-37 0.695122203840377 1103 38-39 0.45469475041611673 1103 40-41 0.47940961384306746 1103 42-43 1.0411641069409328 1103 44-45 1.4634559744880171 1103 46-47 0.3891778464518225 1103 48-49 0.3072266293968724 1103 50-51 1.4263643003288458 1103 52-53 0.8150568711304977 1103 54-55 1.9428089877641597 1103 56-57 1.5123410491696276 1103 58-59 0.09715271834966899 1103 60-61 0.5543770065106557 1103 62-63 -1.3966946300892644 1103 64-65 -1.8883391912318999 1103 66-67 -0.6156690575964419 1103 68-69 0.06073450310948303 1103 70-71 0.0698223479663902 1103 72-73 -0.09457505489631401 1103 74-75 -0.42131958020599036 1103 76-77 -0.3900001518399119 1103 78-79 -0.6371595132582932 1103 80-81 -0.8167332685290738 1103 82-83 -0.17889108586035718 1103 84-85 0.3689496487300321 1103 86-87 0.35887412658077267 1103 88-89 -0.2034221770272442 1103 90-91 0.24265567248594877 1103 92-93 0.4397997261071165 1103 94-95 -0.5231794778359564 1103 96-97 -0.23781387651237118 1103 98-99 -0.8715415914509403 1103 100-101 -1.7052518364688254 1104 1 0.8531901376033701 1104 2 0.6166237895161544 1104 3 0.8395183337695826 1104 4 0.7186820928317701 1104 5 0.7647633604072723 1104 6 0.7549641451103639 1104 7 0.5761662385073265 1104 8 0.6094506464078151 1104 9 1.1207499913282035 1104 10-11 0.7983833414606067 1104 12-13 0.9346985699143318 1104 14-15 1.5634171232957286 1104 16-17 1.4617535811178044 1104 18-19 1.4210907435382651 1104 20-21 1.2734242974046595 1104 22-23 1.2294465879624354 1104 24-25 1.0852027371849928 1104 26-27 0.8166934673187143 1104 28-29 1.027314619605633 1104 30-31 0.7937627307986048 1104 32-33 1.18224727514189 1104 34-35 0.845055328094567 1104 36-37 1.618199126917304 1104 38-39 0.13418192990329914 1104 40-41 -1.5334108989774506 1104 42-43 -1.2280666622898408 1104 44-45 -0.9724414614094137 1104 46-47 -0.05954010226612283 1104 48-49 0.5123548345250768 1104 50-51 1.4135437875083348 1104 52-53 1.8406978967715233 1104 54-55 2.776142321097492 1104 56-57 3.19182822865681 1104 58-59 2.4817681029650522 1104 60-61 2.785146237279889 1104 62-63 2.9366387032440677 1104 64-65 3.7142249113322023 1104 66-67 3.063818121890737 1104 68-69 2.4068883492633297 1104 70-71 3.082642860786901 1104 72-73 2.328501868180613 1104 74-75 1.8991932403068308 1104 76-77 1.827948566108807 1104 78-79 0.32437894828016667 1104 80-81 0.36275391095810505 1104 82-83 0.7954678884986173 1104 84-85 1.2279240077043916 1104 86-87 0.6922074599141048 1104 88-89 1.386321412716347 1104 90-91 1.6657325955628703 1104 92-93 1.3756971620045508 1104 94-95 1.4127179580614815 1104 96-97 1.2750066363081416 1104 98-99 1.7694840495747002 1104 100-101 1.7306455994286107 1105 1 -0.2913320069187719 1105 2 -0.5022573293649657 1105 3 -0.29684530259405406 1105 4 -0.45614308199340314 1105 5 -0.2795256838817721 1105 6 -0.4402573001110781 1105 7 -0.86264495030386 1105 8 -1.0426472556900919 1105 9 -0.14789802731981183 1105 10-11 -0.6500432319659595 1105 12-13 -0.025091639875874705 1105 14-15 -0.7739954641168652 1105 16-17 -0.9875471181828956 1105 18-19 -0.1808906084430859 1105 20-21 -0.10128432730396497 1105 22-23 -0.5747492162333785 1105 24-25 -1.4908462138639607 1105 26-27 -1.356674831049581 1105 28-29 -1.155669063378049 1105 30-31 -1.396213959178091 1105 32-33 -1.9890813961867764 1105 34-35 -0.6199796369403927 1105 36-37 -1.0464279127097385 1105 38-39 -1.2513075805862073 1105 40-41 -0.6855088010753505 1105 42-43 -1.3521925364157141 1105 44-45 -2.870168734136687 1105 46-47 -3.3809270486530743 1105 48-49 -4.936654489484248 1105 50-51 -4.3883210143564675 1105 52-53 -4.212915156841529 1105 54-55 -5.459871664916491 1105 56-57 -4.461726549897968 1105 58-59 -3.9532552070582554 1105 60-61 -5.5079773058436565 1105 62-63 -5.913303021697658 1105 64-65 -5.45389863179134 1105 66-67 -4.9306457475731325 1105 68-69 -3.377493935118954 1105 70-71 -2.62161121846718 1105 72-73 -2.7612417215629783 1105 74-75 -2.9696878785742893 1105 76-77 -4.1181470049867634 1105 78-79 -3.764490515589298 1105 80-81 -3.802455879251685 1105 82-83 -3.223762880732153 1105 84-85 -2.7420643372839546 1105 86-87 -3.3182820505754034 1105 88-89 -3.238678587283653 1105 90-91 -3.3092091293788535 1105 92-93 -3.3466221853147946 1105 94-95 -3.3445664242229025 1105 96-97 -3.473827862526358 1105 98-99 -3.498289843199192 1105 100-101 -2.932233188450178 1106 1 -0.21860473419149784 1106 2 -0.22953005663769233 1106 3 0.1145183337695812 1106 4 0.10522055437022715 1106 5 0.05001977066368113 1106 6 0.03701542716164852 1106 7 0.46462777696886803 1106 8 0.04598910794627642 1106 9 0.3566474272256386 1106 10-11 0.02722949530676999 1106 12-13 0.1010447237604879 1106 14-15 -0.09331364593504787 1106 16-17 0.5704074272716539 1106 18-19 0.23615484610236592 1106 20-21 -0.16151160003123266 1106 22-23 0.040023511039358084 1106 24-25 -1.6840280320457808 1106 26-27 -1.1589475583223106 1106 28-29 -1.2829417906507814 1106 30-31 -0.4655321409962738 1106 32-33 -0.8129450325504166 1106 34-35 -2.009752364213128 1106 36-37 -1.4305188218006393 1106 38-39 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1.1415269364821086 2314 56-57 1.4482384850670655 2314 58-59 0.18048605168300114 2314 60-61 0.3941205962542469 2314 62-63 1.0744592160645823 2314 64-65 1.1212761933834834 2314 66-67 0.7721514552240727 2314 68-69 1.2658627082376874 2314 70-71 1.1948223479663902 2314 72-73 1.4791428938216349 2314 74-75 1.7453470864606775 2314 76-77 1.2926921558523947 2314 78-79 0.37886612776734907 2314 80-81 0.3531385263427218 2314 82-83 0.9973909654216939 2314 84-85 1.5804881102684956 2314 86-87 1.4806689983756431 2314 88-89 1.5786291050240404 2314 90-91 1.5920146468449214 2314 92-93 1.4942869055942953 2314 94-95 1.8774615478050691 2314 96-97 2.3583399696414755 2314 98-99 2.9457661008567513 2314 100-101 2.2338507276337385 2315 1 -0.9095138251005892 2315 2 -0.5568027839104204 2315 3 -0.2695725753213267 2315 4 -0.18341580926613688 2315 5 -0.2431620475181404 2315 6 0.01883360897983266 2315 7 -0.24900858666750025 2315 8 -0.3653745284173624 2315 9 -0.07971620913799171 2315 10-11 -0.45686141378413936 2315 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-0.18100058810597375 2316 34-35 -0.856511623472386 2316 36-37 0.11392562264379791 2316 38-39 -2.604422058700692 2316 40-41 -2.2015020670686205 2316 42-43 -2.883337317560496 2316 44-45 -3.527997016964971 2316 46-47 -3.6599674526934756 2316 48-49 -2.8907790686088255 2316 50-51 -2.61138498742044 2316 52-53 -2.4107266046529787 2316 54-55 -2.671151126195955 2316 56-57 -1.9242686374400577 2316 58-59 -2.581622210425259 2316 60-61 -3.3850816829480337 2316 62-63 -3.5391447725394087 2316 64-65 -4.094891897784606 2316 66-67 -3.737463929391314 2316 68-69 -4.167897975522994 2316 70-71 -5.138867110723069 2316 72-73 -4.22420468452594 2316 74-75 -3.6136272725136838 2316 76-77 -3.3643591261988863 2316 78-79 -3.087301963400744 2316 80-81 -3.0596107614065673 2316 82-83 -3.927466584435857 2316 84-85 -3.504269724489342 2316 86-87 -3.268618750912106 2316 88-89 -2.5859008095058744 2316 90-91 -2.5322731024428276 2316 92-93 -2.806639020331632 2316 94-95 -3.1421253467818246 2316 96-97 -2.851773990472486 2316 98-99 -2.181370651279998 2316 100-101 -2.6860210672380553 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 1.0 4 3.0 5 6.0 6 14.0 7 31.0 8 84.0 9 125.0 10 247.0 11 414.0 12 574.0 13 872.0 14 1074.0 15 1462.0 16 1793.0 17 2266.0 18 2120.0 19 1735.0 20 1597.0 21 1667.0 22 1753.0 23 1710.0 24 1785.0 25 1800.0 26 1377.0 27 736.0 28 595.0 29 619.0 30 629.0 31 758.0 32 898.0 33 984.0 34 1522.0 35 3469.0 36 2973.0 37 3746.0 38 2170.0 39 111.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 47.01859518309279 23.35033507925253 14.837263557558153 14.793806180096523 2 15.528561297554502 26.35371628577311 38.46224235353327 19.655480063139112 3 21.25683435133713 35.44254569578843 34.47258252693707 8.828037425937364 4 10.42062166922074 28.41197593833627 28.581230987397362 32.586171405045626 5 6.1183413005192016 44.31051439811532 33.27691498364631 16.294229317719175 6 18.59289586240022 41.81972050044601 12.110884929438942 27.476498707714832 7 11.927906498021546 46.76013814871572 18.16975823974749 23.142197113515245 8 24.150865716703642 33.38898927288946 17.79236522494911 24.66777978545779 9 20.413531255003317 20.880126255117677 19.098373779190776 39.607968710688226 10-11 21.43821047094074 32.1298689416985 20.415818485396034 26.016102101964734 12-13 29.06040575467167 30.978248438965256 22.833421010498387 17.127924795864686 14-15 24.32583884174653 31.171519407149884 26.87495711443014 17.62768463667345 16-17 14.760641339402117 37.890258685757416 27.180302371857916 20.16879760298255 18-19 13.558701768029094 31.524896502824728 35.86148532741703 19.054916401729148 20-21 15.562315592049586 29.54758582832049 35.733400425424854 19.156698154205074 22-23 14.201413508382698 34.82765718990874 36.0673360627616 14.903593238946957 24-25 15.560384263494967 37.434812442817936 35.60269899359561 11.40210430009149 26-27 14.629515247180471 37.34243817628623 37.35044494772722 10.677601628806075 28-29 12.424235493241234 38.9355229752293 36.58653736190847 12.053704169621007 30-31 15.97401706273873 36.74092541341689 36.03074037647812 11.254317147366253 32-33 12.393499616884528 37.926144485996275 38.52083118903032 11.159524708088883 34-35 11.74526197801695 34.86978302890279 38.78029531859409 14.604659674486168 36-37 9.431825979551226 38.55188819506393 39.34902445161143 12.667261373773417 38-39 11.146187191892851 33.04281188164111 40.67300323691224 15.137997689553808 40-41 10.447214914788974 33.85108086469175 41.122040489534484 14.579663730984787 42-43 12.685850221876574 32.7496683288348 40.64687314149778 13.917608307790841 44-45 10.707913998170174 33.226212259835314 40.762808783165596 15.30306495882891 46-47 14.02072230735802 31.26072139246586 38.388874911369825 16.329681388806293 48-49 13.127558839001852 29.72713341414881 38.92179959287299 18.22350815397635 50-51 9.803069463187027 29.810617323482997 42.154799752979116 18.23151346035086 52-53 11.504768875368816 30.646600032021226 41.66075798815215 16.18787310445781 54-55 10.677935088401455 29.943276686260607 38.69765101438668 20.68113721095126 56-57 14.31005695203678 30.85702522815123 34.89970494727934 19.93321287253265 58-59 12.198943299558564 33.802977973971316 37.75416847739073 16.24391024907939 60-61 12.957160174744404 38.03892866128405 32.48896411335514 16.514947050616406 62-63 9.664692024427621 40.03224994853732 31.86569383133963 18.437364195695434 64-65 9.909425676448388 40.503419409437114 30.83072207863498 18.756432835479515 66-67 11.298918140024245 37.842226847510354 30.302371857917247 20.556483154548157 68-69 11.230301228242721 36.44358546236363 30.878753916882047 21.447359392511608 70-71 12.898835799730108 34.3965142608815 30.5779831202397 22.126666819148692 72-73 12.95029848356625 31.464284897417716 32.66622446879074 22.919192150225292 74-75 12.67125637565472 31.61524210333707 31.147503488026352 24.565998032981863 76-77 12.7604583609707 29.82205347544658 32.68337869673612 24.734109466846597 78-79 13.339127650328217 29.27426179639075 32.93383042473869 24.452780128542347 80-81 13.916653324489376 29.26053841403445 31.77763546122001 25.04517280025617 82-83 13.367718030237185 29.243384186089067 32.28654422359965 25.102353560074107 84-85 13.761279033862833 30.064843723196212 30.810489358538902 25.363387884402055 86-87 13.968116008325518 30.863886919329385 29.75915463964685 25.408842432698247 88-89 13.781706731319046 30.74952539969351 28.303332494682188 27.165435374305254 90-91 14.351555352241538 30.237877401646845 28.421774931381517 26.988792314730098 92-93 14.905880469339678 31.00683881887423 28.1340774456211 25.953203266164998 94-95 14.485030077079664 30.321813316255348 27.829875803389676 27.363280803275313 96-97 13.755403581802794 30.57112142906155 27.90878525193843 27.76468973719723 98-99 14.643992589373529 31.21840763020059 26.297431440268976 27.840168340156907 100-101 14.061892454426935 30.66718510555568 26.726287138903505 28.54463530111388 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1372.0 1 763.0 2 224.5 3 301.0 4 214.5 5 156.5 6 267.5 7 448.0 8 573.5 9 611.5 10 467.5 11 255.5 12 226.5 13 237.0 14 240.5 15 252.5 16 276.0 17 318.5 18 346.0 19 391.5 20 422.0 21 472.5 22 565.0 23 641.5 24 728.5 25 840.5 26 934.5 27 1004.5 28 1081.5 29 1128.0 30 1141.0 31 1157.0 32 1129.5 33 1054.0 34 1007.0 35 934.5 36 796.5 37 680.5 38 623.0 39 625.5 40 664.0 41 701.5 42 754.5 43 842.0 44 887.5 45 878.5 46 955.0 47 1450.5 48 2338.5 49 2488.0 50 2191.5 51 1914.0 52 1164.5 53 502.0 54 280.5 55 234.0 56 188.5 57 187.0 58 163.5 59 118.0 60 84.5 61 64.5 62 58.0 63 54.5 64 50.5 65 50.0 66 42.0 67 30.5 68 26.5 69 23.0 70 25.0 71 24.5 72 16.5 73 13.5 74 10.0 75 4.5 76 6.5 77 5.5 78 2.0 79 1.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.018297843141739666 3 0.018297843141739666 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0022872303927174583 26-27 0.018297843141739666 28-29 0.0 30-31 0.0 32-33 0.0011436151963587291 34-35 0.01257976715994602 36-37 0.004574460785434917 38-39 0.014866997552663479 40-41 0.013723382356304752 42-43 0.006861691178152376 44-45 0.0022872303927174583 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0011436151963587291 86-87 0.0 88-89 0.0 90-91 0.0022872303927174583 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 43721.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 38.121268955421876 #Duplication Level Percentage of deduplicated Percentage of total 1 84.17231655366892 32.087555179433224 2 7.793844123117538 5.942224560279957 3 2.9579408411831762 3.3828137508291207 4 1.2179756404871904 1.8572310788865762 5 0.7619847603047939 1.452391299375586 6 0.4379912401751965 1.0018069120102466 7 0.4139917201655967 1.1047322796825325 8 0.21599568008639825 0.6587223531026279 9 0.24599508009839804 0.8439880149127421 >10 1.4219715605687886 9.889984218110289 >50 0.1379972400551989 3.552068799890213 >100 0.17399652006959862 13.20189382676517 >500 0.011999760004799903 2.7904210791152995 >1k 0.03599928001439971 22.23416664760641 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT 2065 4.723130760961552 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1796 4.107865785320556 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1771 4.050685025502619 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1691 3.867706594085222 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1257 2.8750486036458454 No Hit ATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTT 1141 2.6097298780906204 RNA PCR Primer, Index 33 (95% over 22bp) ACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCT 666 1.5232954415498272 RNA PCR Primer, Index 33 (95% over 24bp) GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 554 1.267125637565472 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 443 1.013243063973834 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 428 0.9789346080830721 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 370 0.8462752453054596 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 365 0.8348390933418723 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 294 0.6724457354589328 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 293 0.6701585050662153 No Hit CATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCT 288 0.6587223531026279 RNA PCR Primer, Index 33 (96% over 27bp) TTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTC 268 0.6129777452482789 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 244 0.5580842158230599 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 196 0.4482971569726218 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 196 0.4482971569726218 No Hit CACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTG 190 0.4345737746163171 RNA PCR Primer, Index 15 (95% over 24bp) GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 170 0.38882916676196794 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 153 0.3499462500857711 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 150 0.34308455890761874 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 143 0.32707394615859653 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 142 0.32478671576587914 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 141 0.32249948537316164 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 141 0.32249948537316164 No Hit TATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCT 138 0.31563779419500926 RNA PCR Primer, Index 33 (95% over 21bp) GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 136 0.31106333340957437 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 133 0.304201642231422 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 121 0.27675487751881245 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 117 0.2676059559479427 No Hit ACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGC 105 0.24015919123533316 RNA PCR Primer, Index 15 (96% over 25bp) CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 103 0.23558473044989822 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 102 0.23329750005718075 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 101 0.2310102696644633 No Hit CTTTACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTC 101 0.2310102696644633 No Hit CTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTC 94 0.2149996569154411 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 93 0.21271242652272362 No Hit GGTATCAACGCAGAGTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 83 0.18984012259554905 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 82 0.18755289220283158 No Hit GGTATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 82 0.18755289220283158 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 81 0.18526566181011414 No Hit TCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCTTG 77 0.1761167402392443 RNA PCR Primer, Index 33 (96% over 29bp) CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 77 0.1761167402392443 No Hit GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACAAGAGGC 75 0.17154227945380937 No Hit AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 68 0.15553166670478719 No Hit GTACCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT 63 0.1440955147411999 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 62 0.14180828434848242 No Hit TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 61 0.13952105395576497 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 60 0.1372338235630475 No Hit CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 59 0.13494659317033003 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 59 0.13494659317033003 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 58 0.1326593627776126 No Hit GTATCAACGCAGAGTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 57 0.13037213238489512 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 55 0.12579767159946023 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 53 0.1212232108140253 No Hit GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC 52 0.11893598042130783 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 51 0.11664875002859038 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 51 0.11664875002859038 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 49 0.11207428924315545 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 48 0.10978705885043802 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 45 0.10292536767228563 No Hit GTATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 44 0.10063813727956816 No Hit CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATATCGTATGCCGT 44 0.10063813727956816 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0022872303927174583 0.0 14-15 0.0 0.0 0.0 0.0022872303927174583 0.0 16-17 0.0 0.0 0.0 0.0022872303927174583 0.0 18-19 0.0 0.0 0.0 0.0022872303927174583 0.0 20-21 0.0 0.0 0.0 0.006861691178152375 0.0 22-23 0.0 0.0 0.0 0.02859037990896823 0.0 24-25 0.0 0.0 0.0 0.07547860295967612 0.0 26-27 0.0 0.0 0.0 0.16239335788293954 0.0 28-29 0.0 0.0 0.0 0.3533770956748473 0.0 30-31 0.0 0.0 0.0 0.6804510418334438 0.0 32-33 0.0 0.0 0.0 1.2236682601038402 0.0 34-35 0.0 0.0 0.0 1.8903959195809796 0.0 36-37 0.0 0.0 0.0 2.67262871389035 0.0 38-39 0.0 0.0 0.0 3.533770956748473 0.0 40-41 0.0 0.0 0.0 4.311429290272409 0.0 42-43 0.0 0.0 0.0 4.99416756249857 0.0 44-45 0.0 0.0 0.0 5.571693236659728 0.0 46-47 0.0 0.0 0.0 6.1377827588573 0.0 48-49 0.0 0.0 0.0 6.581505455044487 0.0 50-51 0.0 0.0 0.0 7.102993984584067 0.0 52-53 0.0 0.0 0.0 7.41520093319 0.0 54-55 0.0 0.0 0.0 7.807460945541044 0.0 56-57 0.0 0.0 0.0 8.302646325564375 0.0 58-59 0.0 0.0 0.0 8.656023421239222 0.0 60-61 0.0 0.0 0.0 8.931634683561676 0.0 62-63 0.0 0.0 0.0 9.207245945884129 0.0 64-65 0.0 0.0 0.0 9.46799021065392 0.0 66-67 0.0 0.0 0.0 9.747032318565449 0.0 68-69 0.0 0.0 0.0 10.080967955902198 0.0 70-71 0.0 0.0 0.0 10.456073740307861 0.0 72-73 0.0 0.0 0.0 10.757988152146567 0.0 74-75 0.0 0.0 0.0 11.063333409574348 0.0 76-77 0.0 0.0 0.0 11.272614990507993 0.0 78-79 0.0 0.0 0.0 11.584821939113926 0.0 80-81 0.0 0.0 0.0 11.862720431829098 0.0 82-83 0.0 0.0 0.0 12.142906154936988 0.0 84-85 0.0 0.0 0.0 12.424235493241234 0.0 86-87 0.0 0.0 0.0 12.668969145262002 0.0 88-89 0.0 0.0 0.0 12.925138949246357 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATGGGGG 50 2.7828404E-5 47.498283 5 CGCAGAA 60 8.162208E-5 39.581905 9 CTTATAC 880 0.0 28.639688 1 TATACAC 955 0.0 26.888435 3 TTATACA 980 0.0 26.202503 2 ATACACA 1085 0.0 23.6397 4 TACACAT 1100 0.0 23.317339 5 GGTATCA 1595 0.0 23.254608 1 CTTGAAA 670 0.0 21.976818 56-57 TGCTTGA 700 0.0 21.374228 54-55 ACACATC 1205 0.0 21.285538 6 CATGGGG 135 3.7168548E-4 21.11035 4 CACATCT 1210 0.0 20.805035 7 TCTGCTT 765 0.0 20.799902 52-53 AGTACTT 1055 0.0 20.48504 12-13 TGAAAAA 725 0.0 20.309612 58-59 CATCTCC 1255 0.0 20.059036 9 ACATCTC 1255 0.0 20.059036 8 CTTCTGC 790 0.0 19.841055 50-51 GCAGAAA 60 0.0047299988 19.790953 10-11 >>END_MODULE