##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938284_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 43837 Sequences flagged as poor quality 0 Sequence length 101 %GC 34 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.05064215160709 33.0 31.0 34.0 30.0 34.0 2 32.25375824075552 33.0 31.0 34.0 30.0 34.0 3 32.189041220886466 34.0 31.0 34.0 30.0 34.0 4 35.78235280698953 37.0 35.0 37.0 35.0 37.0 5 35.812236238793716 37.0 35.0 37.0 35.0 37.0 6 35.9407349955517 37.0 35.0 37.0 35.0 37.0 7 35.889271619864495 37.0 35.0 37.0 35.0 37.0 8 35.910919999087525 37.0 35.0 37.0 35.0 37.0 9 37.40374569427652 39.0 37.0 39.0 34.0 39.0 10-11 37.44234322604193 39.0 37.0 39.0 34.5 39.0 12-13 37.3613842188106 39.0 37.0 39.0 34.0 39.0 14-15 38.185060565275904 40.0 37.5 41.0 33.0 41.0 16-17 38.07759426968086 40.0 37.5 41.0 33.0 41.0 18-19 38.47450783584643 40.0 37.5 41.0 34.0 41.0 20-21 38.57514200333053 40.0 37.5 41.0 35.0 41.0 22-23 38.38976207313456 40.0 37.0 41.0 34.5 41.0 24-25 38.12541916645756 40.0 36.5 41.0 34.0 41.0 26-27 37.666548805803316 40.0 35.5 41.0 33.0 41.0 28-29 37.27051349316787 39.0 35.0 41.0 33.0 41.0 30-31 36.49739945707964 37.5 35.0 40.0 32.5 41.0 32-33 35.53176540365445 36.0 35.0 40.0 31.0 41.0 34-35 34.82026598535484 35.0 35.0 40.0 30.0 41.0 36-37 34.16060633711248 35.0 35.0 40.0 26.0 41.0 38-39 33.38240527408354 35.0 33.5 39.0 22.5 41.0 40-41 32.944795492392274 35.0 33.0 39.0 19.5 41.0 42-43 32.15537103360175 35.0 33.0 39.5 2.0 41.0 44-45 31.19622693158747 35.0 33.0 39.0 2.0 41.0 46-47 29.970549992015876 35.0 31.0 39.0 2.0 41.0 48-49 28.866379998631295 35.0 27.0 39.0 2.0 41.0 50-51 27.74700595387458 35.0 21.0 38.5 2.0 40.5 52-53 26.852476218719346 35.0 4.5 38.0 2.0 40.0 54-55 26.140725870839702 35.0 2.0 38.0 2.0 40.0 56-57 25.11022652097543 34.5 2.0 37.5 2.0 40.0 58-59 23.874877386682485 33.0 2.0 36.5 2.0 40.0 60-61 22.458881766544245 32.0 2.0 35.5 2.0 39.5 62-63 21.233615439012706 31.0 2.0 35.0 2.0 39.0 64-65 19.9692953441157 28.5 2.0 35.0 2.0 39.0 66-67 18.50985468896138 19.5 2.0 35.0 2.0 38.0 68-69 17.417546821178455 2.0 2.0 35.0 2.0 37.0 70-71 16.65758377626206 2.0 2.0 35.0 2.0 36.0 72-73 16.115952277756236 2.0 2.0 34.0 2.0 35.5 74-75 15.704233866368593 2.0 2.0 34.0 2.0 35.0 76-77 14.900483609736067 2.0 2.0 32.0 2.0 35.0 78-79 15.134714966808861 2.0 2.0 33.0 2.0 35.0 80-81 14.91506033715811 2.0 2.0 33.0 2.0 35.0 82-83 14.61050026233547 2.0 2.0 33.0 2.0 35.0 84-85 14.29257248443096 2.0 2.0 33.0 2.0 35.0 86-87 14.034343134794808 2.0 2.0 33.0 2.0 35.0 88-89 13.654732303761662 2.0 2.0 32.5 2.0 35.0 90-91 13.371352966671989 2.0 2.0 32.0 2.0 35.0 92-93 13.005394985970755 2.0 2.0 31.0 2.0 35.0 94-95 12.623731094737323 2.0 2.0 31.0 2.0 35.0 96-97 12.172137691904098 2.0 2.0 31.0 2.0 35.0 98-99 11.577377557770832 2.0 2.0 30.0 2.0 34.5 100-101 9.746401441704496 2.0 2.0 22.5 2.0 32.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.021426088637497287 1101 2 0.2834054107127386 1101 3 0.33618841469387206 1101 4 0.054455836861052376 1101 5 -0.07005522726738889 1101 6 -0.4220282377462041 1101 7 0.24754109618060482 1101 8 -0.1777946737346241 1101 9 -0.23551069108537348 1101 10-11 -0.5296226457034123 1101 12-13 0.18756481181604556 1101 14-15 -0.0375632881741339 1101 16-17 0.23443613385381212 1101 18-19 0.3994997246606573 1101 20-21 0.4936590985813112 1101 22-23 0.6680102413432607 1101 24-25 0.4589218252590399 1101 26-27 0.7022381182450275 1101 28-29 0.9435595414852855 1101 30-31 1.9878428555098253 1101 32-33 1.7989281051893613 1101 34-35 2.2337141192005987 1101 36-37 2.796851808328519 1101 38-39 3.309816746204227 1101 40-41 3.773731278207876 1101 42-43 4.192016064620667 1101 44-45 2.7742026911260638 1101 46-47 3.026721814132433 1101 48-49 4.263017727312537 1101 50-51 4.401023488872436 1101 52-53 5.480467607645075 1101 54-55 5.180379229336435 1101 56-57 4.55429486486063 1101 58-59 5.570491682120803 1101 60-61 4.135395791042825 1101 62-63 4.414759080744158 1101 64-65 4.886736510764486 1101 66-67 4.396734903427184 1101 68-69 2.6288505965187063 1101 70-71 2.7380660353796813 1101 72-73 3.349222859337548 1101 74-75 2.686165746275238 1101 76-77 2.5588934731193707 1101 78-79 1.8836219850738107 1101 80-81 2.973511059268956 1101 82-83 2.8649042392272435 1101 84-85 1.5538503789625135 1101 86-87 2.7124711020026506 1101 88-89 2.459378641247495 1101 90-91 2.0793511381243945 1101 92-93 2.5692613468938834 1101 94-95 2.6738008479171302 1101 96-97 2.2184740450540588 1101 98-99 1.3735337837547217 1101 100-101 0.46517060110839203 1102 1 -0.4539707367593273 1102 2 -0.14913427182694505 1102 3 -0.734446505941051 1102 4 -0.3852267028214911 1102 5 -0.45180125901341484 1102 6 -0.6863139520319166 1102 7 -0.8683319196924089 1102 8 -0.2539851499251 1102 9 -0.37916148473616573 1102 10-11 0.09537735429658767 1102 12-13 -0.35727645802522545 1102 14-15 -0.5292299548408081 1102 16-17 -0.7905638661461865 1102 18-19 -0.3072463070853715 1102 20-21 -0.8984043934821813 1102 22-23 0.6644388127718273 1102 24-25 -0.4204432541060399 1102 26-27 -0.07514283413592437 1102 28-29 -0.8008849029591545 1102 30-31 -1.6808079381409726 1102 32-33 -1.2677385614773087 1102 34-35 -0.6027938173073437 1102 36-37 0.16788355436026592 1102 38-39 0.3804516668391429 1102 40-41 1.2622233416999364 1102 42-43 1.0527303503349472 1102 44-45 1.0900757069990732 1102 46-47 2.452912290322903 1102 48-49 -0.07825211395730491 1102 50-51 1.338721901570846 1102 52-53 -0.32350064632318265 1102 54-55 -1.290255691298487 1102 56-57 0.2023107378765019 1102 58-59 0.30739644402556365 1102 60-61 1.6183322989793325 1102 62-63 1.1849971759822502 1102 64-65 0.09506984409782149 1102 66-67 1.4967349034271855 1102 68-69 2.8082156758837833 1102 70-71 2.6674311147447582 1102 72-73 3.346445081559768 1102 74-75 3.283784793894286 1102 76-77 1.9882585524844476 1102 78-79 1.3550505565023823 1102 80-81 2.0600189957768915 1102 82-83 3.1843486836716863 1102 84-85 3.1840091091212415 1102 86-87 2.9136615781931265 1102 88-89 1.846283403152258 1102 90-91 1.513478122251378 1102 92-93 2.4442613468938834 1102 94-95 2.431340530456813 1102 96-97 1.7926803942604081 1102 98-99 2.570359180580118 1102 100-101 1.961599172536964 1103 1 0.43345616382546837 1103 2 0.44881894454732674 1103 3 0.8459628507840975 1103 4 0.5943054609212055 1103 5 -0.33922815959821406 1103 6 0.06368604796808341 1103 7 0.15505989317308888 1103 8 0.07934818340823568 1103 9 -0.06483399935604695 1103 10-11 0.6414299858755328 1103 12-13 -0.13724721825914088 1103 14-15 -0.6191422355425615 1103 16-17 0.40022560753801883 1103 18-19 0.364537318645624 1103 20-21 0.6293733842955973 1103 22-23 0.8289124969823547 1103 24-25 0.6438842312740789 1103 26-27 0.5717869904254869 1103 28-29 0.8146121730642335 1103 30-31 1.0348353367128311 1103 32-33 -0.5221245263895895 1103 34-35 -0.13057159508511518 1103 36-37 0.5487315075766404 1103 38-39 0.6289896785350564 1103 40-41 0.10944556392216498 1103 42-43 0.1908882450717897 1103 44-45 -0.38068452691905463 1103 46-47 -0.07778946406305565 1103 48-49 -0.4569070847175354 1103 50-51 -1.0655178645110261 1103 52-53 -0.5544947983699657 1103 54-55 -1.3677410714154448 1103 56-57 -1.4592389697258348 1103 58-59 -1.2956737314130287 1103 60-61 -0.9657320285060464 1103 62-63 -0.1198273854212566 1103 64-65 -2.915895068182884 1103 66-67 -3.3716861492043932 1103 68-69 -1.8350591779173868 1103 70-71 -1.752159528530095 1103 72-73 -1.1323560880308747 1103 74-75 -0.5627064341758921 1103 76-77 -0.64185840657988 1103 78-79 -1.8160020750765646 1103 80-81 -2.385887436971645 1103 82-83 -2.306148392351705 1103 84-85 -2.497277440586359 1103 86-87 -2.794671755140209 1103 88-89 -4.348892035444232 1103 90-91 -4.660498485935756 1103 92-93 -3.746528126790327 1103 94-95 -3.6074021596016674 1103 96-97 -2.978518436148949 1103 98-99 -3.343383509478361 1103 100-101 -2.7686639853577724 1104 1 -0.3071453399339319 1104 2 -0.597546970239641 1104 3 -0.3257163472108928 1104 4 0.0592177416229589 1104 5 -1.05100760821977 1104 6 -0.6267901425081064 1104 7 -0.471506522867017 1104 8 -0.3730327689727133 1104 9 -0.1450345006091851 1104 10-11 -0.4939083599891205 1104 12-13 -0.6529113786601499 1104 14-15 -0.023277573888421443 1104 16-17 -0.14175434233666095 1104 18-19 -0.7814526562917266 1104 20-21 -0.8825313776091619 1104 22-23 -0.7938945205615013 1104 24-25 -0.5672686509314389 1104 26-27 -0.11680950080258157 1104 28-29 -1.0588214108956606 1104 30-31 -0.6907285730616053 1104 32-33 -0.3391671329058852 1104 34-35 0.15514269062916952 1104 36-37 -1.203148191671481 1104 38-39 -0.42113563474814697 1104 40-41 -0.9500782456016452 1104 42-43 -1.9770315544269579 1104 44-45 -1.6043687374453697 1104 46-47 -1.4399448525342358 1104 48-49 -0.9036489393541274 1104 50-51 0.4795949174438583 1104 52-53 0.03761046478793162 1104 54-55 0.4041887531459558 1104 56-57 0.3947710553368218 1104 58-59 0.122872634501757 1104 60-61 -0.05031849467146188 1104 62-63 1.9885686045536808 1104 64-65 2.815307939335913 1104 66-67 1.5086396653319447 1104 68-69 2.240755358423467 1104 70-71 1.8856850829987266 1104 72-73 1.8563657164804042 1104 74-75 1.6052133653228555 1104 76-77 2.6469887112146058 1104 78-79 3.247907699359528 1104 80-81 3.373511059268955 1104 82-83 3.900618524941528 1104 84-85 4.418136093248229 1104 86-87 3.0267568162883602 1104 88-89 2.55461673648559 1104 90-91 3.19601780479106 1104 92-93 3.1406899183224564 1104 94-95 3.1833246574409397 1104 96-97 2.7470454736254872 1104 98-99 3.0902004504213867 1104 100-101 2.4437420296798216 1105 1 -0.01593654872513639 1105 2 -0.13417700686967748 1105 3 -0.10410462559917022 1105 4 -0.1587309763257636 1105 5 -0.4795790367911934 1105 6 0.2944552787373169 1105 7 0.4094458580853697 1105 8 0.207553311613367 1105 9 -0.022323877898557498 1105 10-11 -0.38859700467777003 1105 12-13 -0.5677465434953106 1105 14-15 -0.5196145702254213 1105 16-17 -0.6655638661461865 1105 18-19 -0.5177163925554638 1105 20-21 -0.3898573849351763 1105 22-23 -0.524663751330742 1105 24-25 -1.1597594934222784 1105 26-27 -0.42450180849489527 1105 28-29 -0.4974660995403539 1105 30-31 0.467696335363307 1105 32-33 -0.3190206127593598 1105 34-35 -0.4062126207261372 1105 36-37 -0.1536976422209264 1105 38-39 -0.37809534170785497 1105 40-41 0.4620096664862672 1105 42-43 1.036704709309305 1105 44-45 2.389221006144375 1105 46-47 2.8054763928870123 1105 48-49 3.259354723649537 1105 50-51 3.5812432690922122 1105 52-53 3.5247899519674206 1105 54-55 3.3272656762228827 1105 56-57 2.43414834471411 1105 58-59 3.4836784953076183 1105 60-61 1.4057254613724979 1105 62-63 2.5407664067514837 1105 64-65 4.63032625435423 1105 66-67 2.4069913136835943 1105 68-69 1.6116344793025874 1105 70-71 -0.5868423895287478 1105 72-73 0.3133254234401086 1105 74-75 0.6363488964583883 1105 76-77 0.6442414584673539 1105 78-79 -0.08084687939505386 1105 80-81 -1.0147673290094321 1105 82-83 -1.2120188376958332 1105 84-85 -1.2476148225026886 1105 86-87 -1.3139025243709792 1105 88-89 -1.4197422378733826 1105 90-91 -1.086949228175973 1105 92-93 -1.2512514736189377 1105 94-95 -0.19579622167993804 1105 96-97 -0.7511230245430127 1105 98-99 -0.07921346899253123 1105 100-101 0.14429148022927052 1106 1 -0.013912257227161717 1106 2 -0.1564442133474131 1106 3 0.18806811394198775 1106 4 0.5416738819738356 1106 5 0.3186665772438886 1106 6 0.1426334163891383 1106 7 0.23400726159413665 1106 8 -0.052230763960182 1106 9 0.04042915853868578 1106 10-11 0.43090367008606023 1106 12-13 -0.8740893235222984 1106 14-15 0.2361209223521783 1106 16-17 0.4923308706959233 1106 18-19 -0.1486205760912256 1106 20-21 0.48463654219033714 1106 22-23 0.8157546022455122 1106 24-25 1.3544105470635515 1106 26-27 1.5586290956886373 1106 28-29 1.051454278327391 1106 30-31 1.2058879682917834 1106 32-33 1.162085999926198 1106 34-35 1.3957441943885698 1106 36-37 1.8513630865240103 1106 38-39 0.9316212574824263 1106 40-41 1.0962876691853296 1106 42-43 -1.2170064917703165 1106 44-45 -2.3017371584979998 1106 46-47 -1.643578937747268 1106 48-49 -2.496380768928063 1106 50-51 -5.578675759247869 1106 52-53 -5.225547429948911 1106 54-55 -6.170372650362815 1106 56-57 -6.775028443410044 1106 58-59 -4.953568468255138 1106 60-61 -3.5183636074534164 1106 62-63 -2.9224589643686265 1106 64-65 -2.547474015551302 1106 66-67 -2.7532650965728145 1106 68-69 -2.8745328621279125 1106 70-71 -2.923212160109042 1106 72-73 -3.1060402985571898 1106 74-75 -2.7074432762811558 1106 76-77 -2.2997531434219844 1106 78-79 -2.8817915487607753 1106 80-81 -2.464834805392698 1106 82-83 -2.450885234456967 1106 84-85 -3.2341195458495164 1106 86-87 -3.0315138604033667 1106 88-89 -3.2699446670231804 1106 90-91 -3.7920774333041773 1106 92-93 -3.167580758369274 1106 94-95 -3.344244264864825 1106 96-97 -3.9916763308857917 1106 98-99 -3.5802256147415195 1106 100-101 -3.7291903011472467 1107 1 -0.34867357913658736 1107 2 -0.2415115328088575 1107 3 -0.16015710025629915 1107 4 -0.7043481498499133 1107 5 -0.32454027710127065 1107 6 -0.19212790552028736 1107 7 -0.3657479403642441 1107 8 -0.8276285607778107 1107 9 -0.3836834485604541 1107 10-11 -0.29706450616853175 1107 12-13 -0.9034650885161781 1107 14-15 -0.37709817189506367 1107 16-17 -0.10742433126246453 1107 18-19 -0.25265974377787614 1107 20-21 -0.16713824361138307 1107 22-23 0.03943881277182726 1107 24-25 -0.047058241186149985 1107 26-27 -0.5160343070041407 1107 28-29 -0.5076032492123872 1107 30-31 -0.5487019949885124 1107 32-33 -0.8452579413222736 1107 34-35 -0.8573157811316321 1107 36-37 -1.4739123112728052 1107 38-39 -1.8798842504735127 1107 40-41 -2.9545079244499277 1107 42-43 -4.252502207804589 1107 44-45 -3.8543687374453697 1107 46-47 -4.1187931360336805 1107 48-49 -4.138975628169188 1107 50-51 -5.934856909798665 1107 52-53 -6.311226744514393 1107 54-55 -5.250850006543967 1107 56-57 -4.814808125172593 1107 58-59 -4.098611307912417 1107 60-61 -3.0719131790568426 1107 62-63 -3.298529955800692 1107 64-65 -4.148147210165746 1107 66-67 -3.6195441663402548 1107 68-69 -3.1465646858733223 1107 70-71 -2.362930642361185 1107 72-73 -2.5879864429880346 1107 74-75 -2.5669903999041654 1107 76-77 -1.5537311115982373 1107 78-79 -2.6798331644278495 1107 80-81 -2.2842962497011445 1107 82-83 -3.3792818072843858 1107 84-85 -2.7488074283464563 1107 86-87 -2.4981601272332323 1107 88-89 -1.7283289999485163 1107 90-91 -1.9897518519088297 1107 92-93 -1.7563200484549544 1107 94-95 -2.240817092282205 1107 96-97 -2.1217252904941155 1107 98-99 -1.7267984421589002 1107 100-101 -1.0006101297886172 1108 1 -0.22329440825691194 1108 2 0.27719423058852044 1108 3 0.2715921414019462 1108 4 -0.2958547221451582 1108 5 0.14360936900590815 1108 6 0.21585996101156013 1108 7 0.2717646986650806 1108 8 -0.24673877311350623 1108 9 0.6365389983556184 1108 10-11 0.24211648473136904 1108 12-13 0.01893127144337825 1108 14-15 0.24069758139108188 1108 16-17 -0.17643343136357714 1108 18-19 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1.0428492071918924 2311 62-63 -0.10666949068441411 2311 64-65 0.0389104238079625 2311 66-67 -2.1010911835293378 2311 68-69 -1.6640065463384381 2311 70-71 -1.6634867596513772 2311 72-73 -1.6312119232711488 2311 74-75 -0.8865050611781786 2311 76-77 -1.2991810610421215 2311 78-79 -1.721036400019356 2311 80-81 -1.4882901829670683 2311 82-83 -1.1568348912075397 2311 84-85 -0.9669570744536369 2311 86-87 -1.772932624705426 2311 88-89 -0.7316151475723789 2311 90-91 -0.998599172434611 2311 92-93 -1.2785647400626399 2311 94-95 -1.2069444936977742 2311 96-97 -0.642706079169546 2311 98-99 -0.7690128000962115 2311 100-101 -0.7394877839847744 2312 1 -0.03403609623644854 2312 2 0.12374154516652425 2312 3 -0.3243157869868014 2312 4 -0.1074489250437125 2312 5 0.3831660612480192 2312 6 0.4950585969876897 2312 7 0.429053701222621 2312 8 -0.3520243656113706 2312 9 0.25972740415272 2312 10-11 0.6566518641005104 2312 12-13 -0.19142678482261033 2312 14-15 0.2526327902572305 2312 16-17 0.19718123189302617 2312 18-19 0.5154661112152823 2312 20-21 0.5460400509622687 2312 22-23 0.6963015578698659 2312 24-25 0.8017789681161815 2312 26-27 1.098876773707211 2312 28-29 0.6925791493284237 2312 30-31 0.710531931140082 2312 32-33 0.19304575224818166 2312 34-35 0.4919774245227302 2312 36-37 0.9579162340988319 2312 38-39 1.0879680067084294 2312 40-41 1.2417985050986289 2312 42-43 0.8934166248447539 2312 44-45 0.4985724390252173 2312 46-47 -0.02257790575552221 2312 48-49 -0.3070102838919446 2312 50-51 -1.0379120853572594 2312 52-53 -0.619252280310107 2312 54-55 -0.046791639010901775 2312 56-57 -0.528898412450296 2312 58-59 -0.047995712837181514 2312 60-61 1.1485610571492657 2312 62-63 1.1433305093155859 2312 64-65 1.8573247460586018 2312 66-67 1.5065388249958112 2312 68-69 2.0075620811125425 2312 70-71 1.6952088925225368 2312 72-73 0.8962816828669595 2312 74-75 0.6514318527178116 2312 76-77 0.682702996928894 2312 78-79 1.0609329094435598 2312 80-81 0.6270124598291797 2312 82-83 1.0805905137370466 2312 84-85 0.4062313313434647 2312 86-87 0.45532824485979084 2312 88-89 1.2801069325640224 2312 90-91 1.2275304098330775 2312 92-93 -0.4307386531061166 2312 94-95 -2.54496665908567 2312 96-97 -2.5218620893997237 2312 98-99 -1.7172225187662864 2312 100-101 -1.6732047490316635 2313 1 -0.23571676850535894 2313 2 -0.055880303572976686 2313 3 0.007616986122442881 2313 4 -0.04197273456752271 2313 5 0.1156590584469015 2313 6 -0.257742523460486 2313 7 -0.1560303323908201 2313 8 0.3114910405510898 2313 9 -0.1390821196567984 2313 10-11 -0.10105121713198173 2313 12-13 -0.007078045326807114 2313 14-15 0.13743671182586326 2313 16-17 0.27193613385381354 2313 18-19 0.6084282960892295 2313 20-21 0.6293733842955973 2313 22-23 0.6108673842003967 2313 24-25 0.5964218252590427 2313 26-27 0.6897381182450317 2313 28-29 1.2328452557710037 2313 30-31 1.1128428555098253 2313 32-33 1.3364281051893556 2313 34-35 1.8337141192006001 2313 36-37 2.145066094042811 2313 38-39 2.406245317632802 2313 40-41 3.466588421065026 2313 42-43 3.025944636049239 2313 44-45 3.600988405411769 2313 46-47 2.548150385561005 2313 48-49 1.6826605844553946 2313 50-51 2.202809203158143 2313 52-53 2.9751104647879316 2313 54-55 2.014307800765007 2313 56-57 1.9810805791463437 2313 58-59 0.8222773964065162 2313 60-61 1.2443243624713958 2313 62-63 1.5808305093155859 2313 64-65 2.601022225050201 2313 66-67 2.648520617712901 2313 68-69 3.148493453661562 2313 70-71 3.5880660353796827 2313 72-73 3.9813657164804006 2313 74-75 3.2629514605609504 2313 76-77 3.2928220445479415 2313 78-79 4.096121985073813 2313 80-81 4.0342253449832395 2313 82-83 3.847047096370101 2313 84-85 3.694921807533941 2313 86-87 4.330328244859791 2313 88-89 3.709378641247495 2313 90-91 3.8239939952672533 2313 92-93 3.9085470611795987 2313 94-95 3.516657990774272 2313 96-97 3.354188330768345 2313 98-99 3.0663909266118647 2313 100-101 2.2026706011083927 2314 1 -0.28516731795591 2314 2 -0.13417700686967748 2314 3 -0.3028225743171191 2314 4 -0.351038668633457 2314 5 0.1293953221831643 2314 6 -0.2247754904934567 2314 7 0.1530356016751142 2314 8 0.021655875715929085 2314 9 0.3622915067168222 2314 10-11 -0.02962264570341233 2314 12-13 0.23674063599186468 2314 14-15 -0.6317940574049032 2314 16-17 0.03635921077689375 2314 18-19 -0.06258818742725936 2314 20-21 -0.005242000319789497 2314 22-23 0.40482342815644046 2314 24-25 0.25370204503926175 2314 26-27 0.07229306329997343 2314 28-29 0.24932877225451477 2314 30-31 0.3779527456197158 2314 32-33 -0.5049180486567977 2314 34-35 -1.5728792873928086 2314 36-37 -1.6536976422209264 2314 38-39 -1.8107876494001687 2314 40-41 -1.9578621283855284 2314 42-43 -2.437654265049666 2314 44-45 -1.902445660522293 2314 46-47 -1.9092671968565789 2314 48-49 -2.897696558401748 2314 50-51 -1.7456798078308644 2314 52-53 -1.7796972275197618 2314 54-55 -2.310554836597632 2314 56-57 -2.5402106296448608 2314 58-59 -2.6028599662308487 2314 60-61 -2.190428384781349 2314 62-63 -2.3085925676074908 2314 64-65 -3.2574942584662843 2314 66-67 -2.907111250418968 2314 68-69 -2.664006546338438 2314 70-71 -3.4682526459390015 2314 72-73 -4.05846944835476 2314 74-75 -3.3860870009775095 2314 76-77 -3.733963669737774 2314 78-79 -3.5007186742668477 2314 80-81 -2.796818611060715 2314 82-83 -2.78894191461891 2314 84-85 -2.6162045660924313 2314 86-87 -2.7273640628325175 2314 88-89 -1.8812806994118443 2314 90-91 -1.869000510227254 2314 92-93 -2.4115078838753465 2314 94-95 -2.122078272961991 2314 96-97 -2.042789691209677 2314 98-99 -1.3580596228386863 2314 100-101 -1.8749392890014978 2315 1 0.018251485462897676 2315 2 -0.010245382938052217 2315 3 0.0525905310959871 2315 4 -0.014856332451117282 2315 5 -0.12772718493934576 2315 6 -0.38075839647635945 2315 7 -0.2942578456183398 2315 8 0.005274109334159505 2315 9 -0.46249481806950143 2315 10-11 -0.44628931237007663 2315 12-13 -0.36190608765485166 2315 14-15 -0.3023781029889463 2315 16-17 -0.443341643923965 2315 18-19 -0.33039445523352384 2315 20-21 -0.6206266157044027 2315 22-23 -0.46982044648742516 2315 24-25 -0.49451732818010896 2315 26-27 -0.48717987117295536 2315 28-29 0.11578176370750981 2315 30-31 0.23122909889607257 2315 32-33 0.03781699407824135 2315 34-35 -0.8990901136036342 2315 36-37 -1.0034127419360246 2315 38-39 -0.86954833316085 2315 40-41 0.877964082440684 2315 42-43 1.548100720705314 2315 44-45 0.3956312625546303 2315 46-47 -0.8433840059733875 2315 48-49 -0.2449187806239692 2315 50-51 -1.2955373576884135 2315 52-53 -1.3142413870639231 2315 54-55 -0.7069223579651513 2315 56-57 -0.6078744473086815 2315 58-59 -0.8546405930114709 2315 60-61 -0.8862973306502973 2315 62-63 -0.6900028240177463 2315 64-65 -1.011411637383663 2315 66-67 0.1356237923160748 2315 68-69 0.4285860462541571 2315 70-71 0.3248385221521666 2315 72-73 0.07329693341162269 2315 74-75 0.21896997907946947 2315 76-77 0.830851145077041 2315 78-79 1.0309764824283096 2315 80-81 0.7452041809620784 2315 82-83 1.0037931281161327 2315 84-85 1.1747498498619837 2315 86-87 0.8349578744894206 2315 88-89 0.34165377352262816 2315 90-91 0.7542188629921185 2315 92-93 1.3748169024494405 2315 94-95 1.685970160086443 2315 96-97 1.3713840979641105 2315 98-99 2.4870258472467857 2315 100-101 2.318080654018445 2316 1 0.08361096258708045 2316 2 0.006094486342995253 2316 3 0.028625389483785568 2316 4 0.26510009456413286 2316 5 0.3243425318362583 2316 6 -0.19121591281623296 2316 7 0.25258311298733105 2316 8 -0.13633809110157102 2316 9 -0.2892922036904153 2316 10-11 0.0978283346887423 2316 12-13 -0.33848560835201624 2316 14-15 0.17420141770821118 2316 16-17 -0.8028187681069738 2316 18-19 -0.5139456534906017 2316 20-21 0.10486358037402255 2316 22-23 0.30414469512476927 2316 24-25 0.4390338700769689 2316 26-27 0.14789638155036045 2316 28-29 0.6141477767794044 2316 30-31 0.7007280095714492 2316 32-33 -0.28734640461456706 2316 34-35 -0.6354735558694244 2316 36-37 -1.3362014129599942 2316 38-39 -0.5688947383896092 2316 40-41 0.09473968156922297 2316 42-43 -0.22423043397878217 2316 44-45 -0.3543687374453697 2316 46-47 -1.3264994743829739 2316 48-49 -0.8266181270291995 2316 50-51 0.030715365623130708 2316 52-53 -0.8937620842316747 2316 54-55 -0.938948501756002 2316 56-57 -1.381839588920883 2316 58-59 -2.773485908915614 2316 60-61 -1.831831099713476 2316 62-63 -1.640983216174611 2316 64-65 -2.6916948617845335 2316 66-67 -4.032676861278697 2316 68-69 -3.3943987032011833 2316 70-71 -2.461653852575502 2316 72-73 -2.172345768113434 2316 74-75 -1.7799406963017947 2316 76-77 -1.9055322971887545 2316 78-79 -1.5665180709485984 2316 80-81 -1.1965169519355268 2316 82-83 -1.0174487019492275 2316 84-85 -1.1623961196369272 2316 86-87 -0.8583972453362883 2316 88-89 -0.9159714988085259 2316 90-91 -1.1254107666375095 2316 92-93 -0.940542574674744 2316 94-95 -1.2214372473209654 2316 96-97 -0.9728424815565866 2316 98-99 -1.4133009501388365 2316 100-101 -0.5751655333453893 >>END_MODULE >>Per sequence quality scores pass #Quality Count 5 5.0 6 19.0 7 34.0 8 74.0 9 135.0 10 233.0 11 408.0 12 569.0 13 926.0 14 1070.0 15 1491.0 16 1817.0 17 2181.0 18 2143.0 19 1761.0 20 1691.0 21 1804.0 22 1888.0 23 1779.0 24 1866.0 25 1839.0 26 1344.0 27 667.0 28 662.0 29 722.0 30 804.0 31 872.0 32 1110.0 33 1267.0 34 2014.0 35 3777.0 36 2797.0 37 3049.0 38 981.0 39 38.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.97588464785198 23.7115283703315 14.806871848691566 14.505715133124959 2 15.408833728782625 26.3506114254426 38.576838839204235 19.663716006570542 3 21.06764001368769 35.44427968518307 34.49982890384396 8.988251397285275 4 10.09193147341287 28.501037935990148 28.996053562059448 32.41097702853754 5 5.926500444829711 44.21379200218993 33.147797522640694 16.711910030339666 6 18.304589834838943 41.71001003741217 12.3711105027831 27.61428962496578 7 11.969340967675707 46.87136437256199 18.041836804525857 23.117457855236445 8 23.890777197344708 33.18201519264548 18.1764263065447 24.750781303465107 9 20.9799940689372 20.500946688870133 18.922371512649132 39.59668772954353 10-11 21.59591212902343 31.796199557451466 20.392590733854963 26.215297579670143 12-13 29.208203116089148 30.733170609302647 22.98743070921824 17.071195565389967 14-15 24.158815612382234 30.64876702329083 27.248671213814813 17.943746150512123 16-17 14.415904373018225 37.59723521226361 27.46196135684467 20.524899057873487 18-19 13.704176836918586 31.237310947373224 36.06086182904852 18.99765038665967 20-21 15.753815270205534 29.242420786093938 35.99584825603942 19.007915687661107 22-23 14.11478887697607 34.40586718981682 36.4349750211009 15.044368912106213 24-25 15.681958163195475 37.03834660218537 35.84414991901818 11.435545315600978 26-27 14.715108652255745 37.022757086636624 37.55546683397023 10.706667427137399 28-29 12.678225162541349 38.33580472225391 36.81190829246036 12.174061822744383 30-31 16.01898005041576 36.43165927159494 36.244596274708854 11.304764403280446 32-33 12.702739694778383 37.372539179232156 38.69790359741771 11.226817528571754 34-35 11.668491650698057 34.48763573318733 39.08659549228945 14.757277123825165 36-37 9.73378652736273 37.88466364030385 39.67424778155439 12.707302050779024 38-39 11.31122111458357 32.67331249857426 40.93687980473116 15.078586582111003 40-41 10.545885895476424 33.380477678673266 41.41706777379839 14.656568652051918 42-43 12.715286173780141 32.16689098250337 40.83308620571663 14.284736637999861 44-45 10.940529689531674 32.82272965759518 41.085156374751925 15.151584278121222 46-47 14.26078426899651 30.602002874284278 38.80397837443256 16.333234482286652 48-49 13.162847463813577 29.41565626033694 39.05168185602993 18.36981441981955 50-51 9.839506313664206 29.756921076345716 42.324934125725754 18.078638484264317 52-53 11.539909423803058 30.500450599468408 41.61827950856139 16.341360468167142 54-55 10.602206226257971 29.859344520368236 38.6363377100421 20.902111543331696 56-57 14.642800100394734 31.037944645994482 34.44566135030917 19.873593903301618 58-59 12.390612342692847 33.97149930973108 37.22546122514176 16.412427122434313 60-61 12.972362582838109 37.76049092630402 32.253538798462436 17.013607692395432 62-63 9.743074887053348 40.02760918176425 31.570300734723677 18.65901519645872 64-65 10.25784369652025 40.01711351968055 30.757558471192244 18.96748431260696 66-67 11.466021743346376 37.62648444540777 29.92733204804983 20.98016176319602 68-69 11.557639863531076 35.857323794201214 30.616506349912708 21.968529992355002 70-71 13.070709836402216 33.76001095215278 30.243913569260954 22.925365642184044 72-73 12.935459585243994 31.331416968950332 32.2222982684279 23.510825177377775 74-75 13.055729849980036 31.419770691917176 30.414693970680506 25.109805487422282 76-77 12.969871202528035 29.43974810910709 32.77775876427439 24.81262192409049 78-79 13.494363561681347 29.05732280589658 32.60576879193099 24.842544840491076 80-81 13.590992573664456 29.03457637946179 31.69368362213527 25.68074742473848 82-83 13.216331560809733 28.873496588082254 32.26487436383139 25.64529748727663 84-85 13.458532522505049 29.7534427875455 30.837336132440356 25.9506885575091 86-87 13.608096851858189 30.33010417736396 29.760722965802895 26.301076004974956 88-89 13.422857077662037 30.69744092916063 28.02998322855937 27.849718764617965 90-91 14.483427463061213 29.888755776142396 28.37925723087455 27.248559529921845 92-93 14.916994694506247 30.33715557076844 27.95938159621199 26.78646813851332 94-95 14.555597385263013 29.792258461959687 27.67263310403048 27.97951104874682 96-97 13.957653608341507 30.161536881973124 27.531999361153574 28.348810148531793 98-99 15.007814193312877 30.151378606221694 26.070886711308333 28.76992048915709 100-101 14.58654164242611 29.64648711543068 26.32239371228454 29.444577529858663 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1365.0 1 757.5 2 224.5 3 305.5 4 214.5 5 142.0 6 274.0 7 492.0 8 612.0 9 629.0 10 482.0 11 269.0 12 203.0 13 210.5 14 211.5 15 213.0 16 246.0 17 270.5 18 295.5 19 330.5 20 392.5 21 459.5 22 522.5 23 603.5 24 689.0 25 766.5 26 856.5 27 960.5 28 1037.5 29 1097.5 30 1143.5 31 1138.5 32 1152.0 33 1146.5 34 1053.0 35 983.5 36 890.5 37 737.0 38 630.5 39 608.0 40 642.5 41 699.5 42 759.0 43 837.0 44 896.5 45 922.5 46 1000.5 47 1457.0 48 2277.5 49 2459.5 50 2321.0 51 2057.0 52 1212.5 53 530.5 54 286.0 55 231.0 56 228.5 57 217.0 58 151.5 59 100.5 60 70.5 61 55.0 62 59.0 63 62.5 64 53.0 65 43.5 66 46.5 67 50.0 68 39.5 69 24.5 70 20.0 71 17.5 72 18.5 73 14.5 74 10.0 75 8.5 76 7.5 77 6.0 78 4.0 79 3.5 80 1.0 81 1.0 82 0.5 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.01368706800191619 2 0.011405890001596825 3 0.00456235600063873 4 0.0 5 0.0 6 0.002281178000319365 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.010265301001437141 28-29 0.00456235600063873 30-31 0.0034217670004790474 32-33 0.0 34-35 0.002281178000319365 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0034217670004790474 50-51 0.007984123001117777 52-53 0.014827657002075873 54-55 0.014827657002075873 56-57 0.02281178000319365 58-59 0.030795903004311426 60-61 0.0034217670004790474 62-63 0.025092958003513015 64-65 0.02737413600383238 66-67 0.01710883500239524 68-69 0.03992061500558889 70-71 0.02281178000319365 72-73 0.00912471200127746 74-75 0.02167119100303397 76-77 0.019390013002714604 78-79 0.03421767000479048 80-81 0.014827657002075873 82-83 0.0456235600063873 84-85 0.030795903004311426 86-87 0.03992061500558889 88-89 0.02851472500399206 90-91 0.03307708100463079 92-93 0.03307708100463079 94-95 0.019390013002714604 96-97 0.01824942400255492 98-99 0.014827657002075873 100-101 0.012546479001756507 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 43837.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 37.6485617172708 #Duplication Level Percentage of deduplicated Percentage of total 1 83.93116820164809 31.59887766042384 2 8.119243819680078 6.1135570408558975 3 2.7872031022782355 3.1480256404407236 4 1.3269510421715947 1.9983119282797637 5 0.648327678138633 1.2204302301708603 6 0.4847309743092583 1.0949654401532953 7 0.3271934076587494 0.8622852841207199 8 0.3271934076587494 0.9854688961379656 9 0.17571497818710616 0.5953874580833542 >10 1.5147842947164325 10.331455163446405 >50 0.13936015511391178 3.814129616533978 >100 0.16965584100824044 13.173802951844332 >500 0.012118274357731459 2.8856901704039966 >1k 0.036354823073194376 22.177612519104866 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT 2041 4.655884298651824 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1825 4.163149850582841 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1813 4.135775714579008 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1682 3.836941396537172 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1232 2.8104112963934575 No Hit ATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTT 1129 2.575449962360563 RNA PCR Primer, Index 33 (95% over 22bp) ACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCT 729 1.6629787622328172 RNA PCR Primer, Index 33 (95% over 24bp) GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 536 1.2227114081711796 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 468 1.0675913041494627 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 425 0.9695006501357301 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 394 0.8987841321258297 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 391 0.8919405981248716 No Hit CATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCT 316 0.7208522481009193 RNA PCR Primer, Index 33 (96% over 27bp) GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 283 0.6455733740903803 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 277 0.6318863060884641 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 268 0.6113557040855898 No Hit TTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTC 243 0.5543262540776057 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 239 0.5452015420763282 No Hit CACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTG 192 0.43798617606131807 RNA PCR Primer, Index 15 (95% over 24bp) GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 187 0.42658028605972126 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 172 0.3923626160549308 No Hit TATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCT 167 0.38095672605333397 RNA PCR Primer, Index 33 (95% over 21bp) GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 159 0.362707302050779 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 149 0.3398955220475854 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 141 0.32164609804503047 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 137 0.31252138604375296 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 134 0.3056778520427949 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 129 0.2942719620411981 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 120 0.27374136003832383 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 119 0.2714601820380044 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 119 0.2714601820380044 No Hit CTTTACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTC 116 0.2646166480370463 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 111 0.2532107580354495 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 108 0.2463672240344914 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 108 0.2463672240344914 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 103 0.2349613340328946 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 98 0.2235554440312978 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 97 0.2212742660309784 No Hit ACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGC 94 0.21443073203002028 RNA PCR Primer, Index 15 (96% over 25bp) CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 90 0.20530602002874285 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 88 0.20074366402810412 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 83 0.18933777402650728 No Hit TCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCTTG 82 0.1870565960261879 RNA PCR Primer, Index 33 (96% over 29bp) AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 79 0.18021306202522983 No Hit GGTATCAACGCAGAGTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 79 0.18021306202522983 No Hit GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACAAGAGGC 78 0.17793188402491047 No Hit CTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTC 73 0.16652599402331364 No Hit TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 70 0.15968246002235556 No Hit CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 67 0.15283892602139745 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 66 0.15055774802107807 No Hit GTATCAACGCAGAGTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 64 0.14599539202043935 No Hit GGTATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 63 0.14371421402011997 No Hit GTACCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT 62 0.14143303601980062 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 62 0.14143303601980062 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 60 0.13687068001916192 No Hit GTATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 57 0.1300271460182038 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 57 0.1300271460182038 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 52 0.11862125601660699 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 51 0.11634007801628761 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 50 0.11405890001596825 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 48 0.10949654401532952 No Hit GGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 48 0.10949654401532952 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 46 0.10493418801469079 No Hit TATCAACGCAGAGTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 45 0.10265301001437142 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 44 0.10037183201405206 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.002281178000319365 0.0 16-17 0.0 0.0 0.0 0.002281178000319365 0.0 18-19 0.0 0.0 0.0 0.002281178000319365 0.0 20-21 0.0 0.0 0.0 0.007984123001117777 0.0 22-23 0.0 0.0 0.0 0.04220179300590825 0.0 24-25 0.0 0.0 0.0 0.0844035860118165 0.0 26-27 0.0 0.0 0.0 0.19846248602778477 0.0 28-29 0.0 0.0 0.0 0.3969249720555695 0.0 30-31 0.0 0.0 0.0 0.7162898921002806 0.0 32-33 0.0 0.0 0.0 1.153135479161439 0.0 34-35 0.0 0.0 0.0 1.864863015261081 0.0 36-37 0.0 0.0 0.0 2.754522435385633 0.0 38-39 0.0 0.0 0.0 3.638478910509387 0.0 40-41 0.0 0.0 0.0 4.469968291625795 0.0 42-43 0.0 0.0 0.0 5.1520405137212855 0.0 44-45 0.0 0.0 0.0 5.7611150398065565 0.0 46-47 0.0 0.0 0.0 6.343956018888154 0.0 48-49 0.0 0.0 0.0 6.852658712959372 0.0 50-51 0.0 0.0 0.0 7.274676643018455 0.0 52-53 0.0 0.0 0.0 7.610009809065401 0.0 54-55 0.0 0.0 0.0 8.013778315121929 0.0 56-57 0.0 0.0 0.0 8.55441750119762 0.0 58-59 0.0 0.0 0.0 8.90115655724616 0.0 60-61 0.0 0.0 0.0 9.174897917284486 0.0 62-63 0.0 0.0 0.0 9.441795743321851 0.0 64-65 0.0 0.0 0.0 9.685881789356024 0.0 66-67 0.0 0.0 0.0 10.001824942400255 0.0 68-69 0.0 0.0 0.0 10.409015215457263 0.0 70-71 0.0 0.0 0.0 10.80936195451331 0.0 72-73 0.0 0.0 0.0 11.123023929557224 0.0 74-75 0.0 0.0 0.0 11.477747108606884 0.0 76-77 0.0 0.0 0.0 11.727536099641856 0.0 78-79 0.0 0.0 0.0 12.03435454068481 0.0 80-81 0.0 0.0 0.0 12.304674133722655 0.0 82-83 0.0 0.0 0.0 12.57157195976002 0.0 84-85 0.0 0.0 0.0 12.841891552797865 0.0 86-87 0.0 0.0 0.0 13.0700093528298 0.0 88-89 0.0 0.0 0.0 13.375687204872595 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACCCA 25 0.0049998662 56.969177 1 CAGCACC 25 0.001602926 38.022858 86-87 CTTTACA 80 1.0580739E-5 35.60574 1 CGTTGCG 35 0.008372119 27.12818 22-23 CTTATAC 845 0.0 26.40583 1 TATACAC 910 0.0 25.041397 3 CATGGGG 95 0.0012116286 24.98648 4 TTACACA 135 1.3102732E-5 24.616312 3 GGTATCA 1715 0.0 23.806362 1 TTATACA 965 0.0 23.122206 2 AGTACTT 945 0.0 22.606817 12-13 GAGTAAA 85 3.108864E-6 22.340855 10-11 AGTACAA 130 2.8900232E-4 21.911222 7 GGCTTGA 55 0.0028654092 21.579233 20-21 GTACTTT 1020 0.0 21.409985 14-15 ATACACA 1070 0.0 21.29689 4 TACACAT 1110 0.0 20.95713 5 TACTTTT 1035 0.0 20.870352 14-15 CTTGAAA 655 0.0 20.68037 56-57 GAGTACT 1195 0.0 20.459642 12-13 >>END_MODULE