FastQCFastQC Report
Thu 26 May 2016
SRR938283_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938283_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2251891
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83880.37248694541609695No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69970.3107166377058215No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41720.18526651600810165No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA42450.054.9572681
GTATCAA72500.041.193651
ATCAACG92400.031.7332613
TCAACGC93250.031.4440044
TATCAAC93950.031.3614732
CAACGCA94250.031.0599615
AACGCAG97250.030.1459886
TGGTATC18150.026.9688322
ACGCAGA111500.026.2856567
CGCAGAG112050.025.987068
GCAGAGT120400.023.8686479
GTGGTAT21500.023.2994041
GTACATG72900.023.2324371
GAGTACT74200.023.04661612-13
TACATGG71850.022.620432
GTACTTT76900.022.11487614-15
CAGAGTA116850.021.74847210-11
GTGTACG2509.03492E-920.9916531
AGAGTAC110500.020.91338310-11
GTAAGGT10600.020.174754
Produced by FastQC (version 0.11.5)