FastQCFastQC Report
Thu 26 May 2016
SRR938283_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938283_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2251891
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80200.35614512425334977No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71060.3155570140828308No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42200.18739805789889474No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA42750.055.2862051
GTATCAA72150.041.1286771
ATCAACG91200.032.273353
TCAACGC91700.032.0966644
TATCAAC92900.031.7886712
CAACGCA94950.031.0481225
AACGCAG98000.030.1924826
TGGTATC18450.028.8680972
GTGGTAT20800.028.1214891
CGCAGAG111200.026.4376438
ACGCAGA112450.026.1859957
GTGTACG2101.2496457E-924.9097821
TACATGG73350.024.3772242
GTACATG75700.024.185851
GCAGAGT124150.023.2973989
GAGTACT78000.022.95533412-13
ACATGGG79200.022.1538623
GTACTTT81500.021.61987314-15
CAGAGTA119450.021.25193210-11
AGAGTAC112550.020.57150310-11
Produced by FastQC (version 0.11.5)