FastQCFastQC Report
Thu 26 May 2016
SRR938282_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938282_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2230875
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83920.3761752675519695No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67120.30086849330419674No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42820.19194262341009694No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA41000.051.576841
GTATCAA75100.043.666831
ATCAACG93050.034.6827283
TATCAAC94250.034.5437132
TCAACGC93650.034.4605184
CAACGCA95300.033.863885
AACGCAG98750.032.769696
TGGTATC18250.029.949862
ACGCAGA112850.028.415647
CGCAGAG113650.028.1320178
GTGGTAT23200.025.1019041
GCAGAGT123800.025.0575169
GTACATG72150.022.6268771
GAGTACT77100.022.58340612-13
TACATGG71950.022.1956732
CAGAGTA119100.021.41991610-11
GTACTTT81250.021.25497614-15
AGAGTAC111600.021.19924410-11
TAAGGTG10550.021.174115
ACATGGG75350.020.8787523
Produced by FastQC (version 0.11.5)