##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938282_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2230875 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.820871631086458 33.0 31.0 34.0 30.0 34.0 2 32.01429573597804 33.0 31.0 34.0 30.0 34.0 3 32.09597534599653 34.0 31.0 34.0 30.0 34.0 4 35.622167086905364 37.0 35.0 37.0 33.0 37.0 5 35.622946601669746 37.0 35.0 37.0 33.0 37.0 6 35.64056614557069 37.0 35.0 37.0 33.0 37.0 7 35.587010926206084 37.0 35.0 37.0 33.0 37.0 8 35.55896632487253 37.0 35.0 37.0 33.0 37.0 9 37.193113016193195 39.0 37.0 39.0 34.0 39.0 10-11 37.10621953269457 39.0 37.0 39.0 33.5 39.0 12-13 36.993463327169835 39.0 37.0 39.0 33.0 39.0 14-15 38.17374595170057 40.0 38.0 41.0 33.0 41.0 16-17 38.01545133635905 40.0 38.0 41.0 33.0 41.0 18-19 38.15879397097551 40.0 38.0 41.0 33.0 41.0 20-21 38.14419611139127 40.0 38.0 41.0 33.0 41.0 22-23 38.05378203619656 40.0 38.0 41.0 33.0 41.0 24-25 37.94433888048411 40.0 38.0 41.0 33.0 41.0 26-27 37.76440970471228 40.0 38.0 41.0 32.5 41.0 28-29 37.56105026054799 40.0 37.5 41.0 32.0 41.0 30-31 37.347507368185134 40.0 37.0 41.0 31.5 41.0 32-33 37.10290379335463 40.0 36.5 41.0 31.0 41.0 34-35 36.87268874320614 39.5 36.0 41.0 30.5 41.0 36-37 36.61145514652323 39.0 36.0 41.0 30.0 41.0 38-39 36.32595237294784 39.0 35.0 40.5 30.0 41.0 40-41 36.113782036196554 39.0 35.0 40.0 30.0 41.0 42-43 35.94383795595898 39.0 35.0 40.0 28.5 41.0 44-45 35.675020787807476 39.0 35.0 40.0 27.5 41.0 46-47 35.300830391662466 38.5 35.0 40.0 26.0 41.0 48-49 34.92353852188043 38.0 34.0 40.0 24.5 41.0 50-51 34.229846136605595 37.5 33.5 39.5 24.0 40.5 52-53 34.3677917857343 38.0 34.0 40.0 24.0 40.5 54-55 34.47828408135821 38.0 34.0 40.0 23.5 41.0 56-57 34.173937132291144 38.0 34.0 40.0 23.0 41.0 58-59 33.85135003081751 38.0 33.5 40.0 22.0 41.0 60-61 33.42578248445117 37.0 33.0 40.0 20.5 41.0 62-63 32.879067854541375 36.5 32.0 39.5 17.5 41.0 64-65 32.35567277413571 36.0 31.5 39.0 13.5 40.5 66-67 31.806914103210623 35.5 31.0 39.0 8.0 40.0 68-69 31.183601501652937 35.0 30.5 38.0 6.5 40.0 70-71 30.618793074466296 35.0 29.5 37.5 2.0 40.0 72-73 29.957385554995238 34.5 29.0 37.0 2.0 39.0 74-75 29.24310214601894 34.0 28.5 36.0 2.0 39.0 76-77 28.5526190396145 34.0 26.5 36.0 2.0 38.5 78-79 27.811661119515882 33.5 26.0 35.0 2.0 37.0 80-81 27.13262307390598 33.0 25.5 35.0 2.0 37.0 82-83 26.380271642292822 32.0 23.5 35.0 2.0 36.0 84-85 25.698004818737044 32.0 21.0 35.0 2.0 36.0 86-87 24.98631276965316 31.0 19.0 34.0 2.0 35.0 88-89 24.158916120356363 31.0 11.0 34.0 2.0 35.0 90-91 23.303758390765957 30.5 4.5 34.0 2.0 35.0 92-93 22.379137558133017 29.5 2.0 34.0 2.0 35.0 94-95 21.39335865971872 29.0 2.0 34.0 2.0 35.0 96-97 20.162421471395753 28.0 2.0 34.0 2.0 35.0 98-99 18.65183459404942 25.5 2.0 33.0 2.0 35.0 100-101 15.042412506303581 10.0 2.0 29.5 2.0 33.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.40626650146208476 1101 2 0.3759095738909437 1101 3 0.39727541164483426 1101 4 0.3078582114383934 1101 5 0.3426365160934566 1101 6 0.33474376589082766 1101 7 0.28108784769364803 1101 8 0.3673489646806516 1101 9 0.41944309247851663 1101 10-11 0.4230688816262216 1101 12-13 0.48935900429808044 1101 14-15 0.6287993029378285 1101 16-17 0.6675278729802869 1101 18-19 0.7264965605029303 1101 20-21 0.7112043592349266 1101 22-23 0.7282840852660541 1101 24-25 0.7742480651025616 1101 26-27 0.7995861654558425 1101 28-29 0.898585009180934 1101 30-31 0.9341077571404881 1101 32-33 0.8392306542629768 1101 34-35 0.975722409156532 1101 36-37 0.9118071444845839 1101 38-39 1.0536159273412977 1101 40-41 1.1025644412298163 1101 42-43 1.2404384959242876 1101 44-45 1.2079996303695637 1101 46-47 1.5066706180744802 1101 48-49 1.486011213183133 1101 50-51 1.3766437393980695 1101 52-53 1.4314151503501265 1101 54-55 1.5754027602191911 1101 56-57 1.6265901854978893 1101 58-59 1.6174695648393609 1101 60-61 1.5717401943078855 1101 62-63 1.6650986304815873 1101 64-65 1.6013390615232836 1101 66-67 1.793947783798373 1101 68-69 1.9538326588267871 1101 70-71 2.168531493237424 1101 72-73 2.28344555031849 1101 74-75 2.419044761140338 1101 76-77 2.488016755003027 1101 78-79 2.7018549853856584 1101 80-81 2.9003220626688275 1101 82-83 3.0688098151154684 1101 84-85 3.264258385551198 1101 86-87 3.5940032989665696 1101 88-89 3.83174882944207 1101 90-91 4.047866622960335 1101 92-93 4.246326640677399 1101 94-95 4.545863829378916 1101 96-97 4.569455194359261 1101 98-99 5.024606219162063 1101 100-101 4.920571716817889 1102 1 0.39734605978363646 1102 2 0.2295186943232821 1102 3 0.2028199323681008 1102 4 0.21746762159562394 1102 5 0.26440140568390547 1102 6 0.2824374685548463 1102 7 0.12863443002414243 1102 8 0.25290830250725804 1102 9 0.4581519925097908 1102 10-11 0.42811604128274183 1102 12-13 0.37045465621150697 1102 14-15 0.4419002557437395 1102 16-17 0.5193372787111628 1102 18-19 0.44328265299267855 1102 20-21 0.5198514624666402 1102 22-23 0.4970754607640444 1102 24-25 0.43759209843523905 1102 26-27 0.6537106876948044 1102 28-29 0.6318139953517061 1102 30-31 0.6382719265320773 1102 32-33 0.6927985291375904 1102 34-35 0.7043548070614776 1102 36-37 0.7443234655417967 1102 38-39 0.7163304349799944 1102 40-41 0.8352513109915449 1102 42-43 1.0390691881186385 1102 44-45 1.022664561071437 1102 46-47 1.0696482254252615 1102 48-49 1.2123616922240146 1102 50-51 1.2331823004176812 1102 52-53 1.0014250694037656 1102 54-55 1.1092942014641665 1102 56-57 1.1104960347439174 1102 58-59 1.2939410867378527 1102 60-61 1.221742128042706 1102 62-63 1.2915455503549893 1102 64-65 1.365200866326056 1102 66-67 1.4730146460889557 1102 68-69 1.7962832351356504 1102 70-71 1.797367078608012 1102 72-73 1.9611178880071627 1102 74-75 1.9056554918260709 1102 76-77 2.2279999527476413 1102 78-79 2.435584395301447 1102 80-81 2.364499792795005 1102 82-83 2.5309900977716104 1102 84-85 2.6440580998055516 1102 86-87 2.740922434647466 1102 88-89 2.9744529820984695 1102 90-91 3.1724673290753422 1102 92-93 3.781885788483816 1102 94-95 4.092812004615084 1102 96-97 4.103668105045973 1102 98-99 4.173255544512163 1102 100-101 3.6866698356404193 1103 1 0.5462818309821387 1103 2 0.648816332984282 1103 3 0.5539882860253726 1103 4 0.5056270020415354 1103 5 0.3926501991511344 1103 6 0.4286601154870553 1103 7 0.4616733027438187 1103 8 0.42335425358984935 1103 9 0.490226926578039 1103 10-11 0.5913409895981872 1103 12-13 0.6478912758036586 1103 14-15 0.8499669650680133 1103 16-17 0.8733585694451165 1103 18-19 0.9547264504821484 1103 20-21 0.9814545821816694 1103 22-23 0.8511493737369378 1103 24-25 0.978314433832594 1103 26-27 1.0682181631316965 1103 28-29 1.0840688302998132 1103 30-31 1.073628305047201 1103 32-33 1.1241217489244448 1103 34-35 1.0951528848942473 1103 36-37 1.2025184555687645 1103 38-39 1.3142462864218203 1103 40-41 1.3593128297477364 1103 42-43 1.5713373969527922 1103 44-45 1.607660813136441 1103 46-47 1.627069584568126 1103 48-49 1.5661874689478594 1103 50-51 1.617063772743144 1103 52-53 1.6566388416513576 1103 54-55 1.7443466585588538 1103 56-57 1.8355529535326909 1103 58-59 1.9051444733707967 1103 60-61 1.9070017991984969 1103 62-63 1.9777888095301535 1103 64-65 1.967268416486526 1103 66-67 2.1389478203239918 1103 68-69 2.4239225343193844 1103 70-71 2.5592041433619173 1103 72-73 2.610790930361901 1103 74-75 2.65770440027849 1103 76-77 2.8324808483557575 1103 78-79 3.081498866249216 1103 80-81 3.2278087687494406 1103 82-83 3.5203958135544227 1103 84-85 3.276000106739371 1103 86-87 3.359150050462574 1103 88-89 4.162020754224024 1103 90-91 4.250322071542662 1103 92-93 4.4103850973970715 1103 94-95 4.775004331061062 1103 96-97 4.82206551155333 1103 98-99 5.143425452764728 1103 100-101 4.810413549662062 1104 1 0.1495722834594666 1104 2 0.18301049224395172 1104 3 0.1562302738145931 1104 4 0.10287700420620638 1104 5 0.1996522217176988 1104 6 0.23016693012552736 1104 7 0.04146587125055845 1104 8 0.04774031473950657 1104 9 0.25774637168886727 1104 10-11 0.21576397748571452 1104 12-13 0.1781249787609056 1104 14-15 0.24808227530099458 1104 16-17 0.27473807597147015 1104 18-19 0.27832143903995643 1104 20-21 0.2422831570591626 1104 22-23 0.23547738605467572 1104 24-25 0.2413728743425949 1104 26-27 0.3293730939717321 1104 28-29 0.4431462119343479 1104 30-31 0.4125322277873025 1104 32-33 0.24851850609472592 1104 34-35 0.39456671749745453 1104 36-37 0.5050475041781013 1104 38-39 0.5695382699581018 1104 40-41 0.5133543720731595 1104 42-43 0.47643351597810835 1104 44-45 0.6417129954557126 1104 46-47 0.710406228574513 1104 48-49 0.8837566505545738 1104 50-51 0.8559876613884043 1104 52-53 0.8641396043794956 1104 54-55 1.0785629229278157 1104 56-57 1.1180261994744427 1104 58-59 1.1000590758328102 1104 60-61 1.298242624177476 1104 62-63 1.3466219806405064 1104 64-65 1.3994739817788613 1104 66-67 1.307338965648782 1104 68-69 1.3886922617287603 1104 70-71 1.7135920330217687 1104 72-73 1.8994457122216595 1104 74-75 2.0547754478314673 1104 76-77 1.9861016805367235 1104 78-79 2.2135784222319295 1104 80-81 2.2293897424642246 1104 82-83 2.4006305606470093 1104 84-85 2.531993443627858 1104 86-87 2.7092952362091616 1104 88-89 2.9530477606574266 1104 90-91 3.293266976340437 1104 92-93 3.4765361649363307 1104 94-95 3.5804996153893 1104 96-97 3.5803708442326787 1104 98-99 3.773552475649627 1104 100-101 3.719782601592261 1105 1 0.31713631993852687 1105 2 0.2532603098449826 1105 3 0.12224280608624127 1105 4 0.051597599156131935 1105 5 0.09976031817152631 1105 6 0.18159140812230135 1105 7 0.2110428270586837 1105 8 0.1291073632758426 1105 9 0.21691457352385868 1105 10-11 0.1634271551167359 1105 12-13 0.07640356664887804 1105 14-15 0.26257098974424053 1105 16-17 0.2961676092619072 1105 18-19 0.21653243169138392 1105 20-21 0.19750342841879132 1105 22-23 0.11441680825742395 1105 24-25 0.19231251877223343 1105 26-27 0.2106797475696922 1105 28-29 0.1813252318769969 1105 30-31 0.13195073656397227 1105 32-33 0.39786941227635 1105 34-35 0.2608963277946543 1105 36-37 0.3507137856269722 1105 38-39 0.3891249941829926 1105 40-41 0.49031912078202566 1105 42-43 0.4187028166955926 1105 44-45 0.49082002235068245 1105 46-47 0.559972334265467 1105 48-49 0.6277194562499062 1105 50-51 0.6798872060072583 1105 52-53 0.6377817484568382 1105 54-55 0.7446111991425823 1105 56-57 0.7480243267346509 1105 58-59 0.8815721539216455 1105 60-61 0.9399634034579805 1105 62-63 0.9067089655404814 1105 64-65 0.9378571450755118 1105 66-67 1.2468300624083568 1105 68-69 1.3966115779175077 1105 70-71 1.3154402873817759 1105 72-73 1.3645966554165696 1105 74-75 1.602103611078455 1105 76-77 1.6978464632639536 1105 78-79 1.7794735679358702 1105 80-81 1.8847453735425788 1105 82-83 2.2517727663518414 1105 84-85 2.2125187526575196 1105 86-87 2.3939565775249996 1105 88-89 2.5821500817877805 1105 90-91 2.683976512927483 1105 92-93 3.0796338936230647 1105 94-95 3.395036380877027 1105 96-97 3.4756980273266542 1105 98-99 3.7338396303103387 1105 100-101 3.8548426451619733 1106 1 0.2938376462120402 1106 2 0.06773622260582357 1106 3 0.13565645762290046 1106 4 0.21938207011752553 1106 5 0.22554874657669188 1106 6 -0.006080770828695847 1106 7 0.15947711540395915 1106 8 0.225614179177974 1106 9 0.1015141609703889 1106 10-11 0.20647047688468234 1106 12-13 0.35149944239317676 1106 14-15 0.4634397637588492 1106 16-17 0.28721265944250973 1106 18-19 0.30003157205274533 1106 20-21 0.29113141408721077 1106 22-23 0.2655510562243464 1106 24-25 0.08072384078865724 1106 26-27 0.300930933797936 1106 28-29 0.15304449088858973 1106 30-31 0.2654503336939982 1106 32-33 0.28961532830764725 1106 34-35 0.3456296571084181 1106 36-37 0.3648395395236719 1106 38-39 0.1357767884616976 1106 40-41 0.4393903273638742 1106 42-43 0.4661226218198138 1106 44-45 0.5229968174058897 1106 46-47 0.751190591126587 1106 48-49 0.8764685495640947 1106 50-51 0.7260690766759481 1106 52-53 0.7414438456097088 1106 54-55 0.46772341989705524 1106 56-57 0.7710880386561314 1106 58-59 0.9169737358029124 1106 60-61 0.9140567633204384 1106 62-63 0.853861926406374 1106 64-65 1.0696130734761127 1106 66-67 1.2884353850423338 1106 68-69 1.0018469690850615 1106 70-71 1.1459094070789213 1106 72-73 1.4419273175667087 1106 74-75 1.6185641837202027 1106 76-77 1.7893338482267644 1106 78-79 2.1071211361908126 1106 80-81 2.2877778607576253 1106 82-83 2.3236202860065944 1106 84-85 2.546197037692135 1106 86-87 2.340339426409482 1106 88-89 2.4638534817910696 1106 90-91 2.698866714874047 1106 92-93 3.0577797964994247 1106 94-95 3.4422958601972837 1106 96-97 3.543193729133449 1106 98-99 3.892525908796717 1106 100-101 3.878817336523383 1107 1 0.15799111431351065 1107 2 0.09502208070535545 1107 3 0.06976596871844976 1107 4 0.09920749264877315 1107 5 0.15394072051512353 1107 6 0.15947019241124138 1107 7 0.14150539045100885 1107 8 0.07922922085271722 1107 9 0.22980483995919343 1107 10-11 0.13462109372090936 1107 12-13 0.17010394814261076 1107 14-15 0.33026629303121524 1107 16-17 0.47364538896624 1107 18-19 0.43596689335156924 1107 20-21 0.41095333506465437 1107 22-23 0.3720326824459832 1107 24-25 0.39420919991677295 1107 26-27 0.41582004083918633 1107 28-29 0.4148487915470085 1107 30-31 0.4948633857358473 1107 32-33 0.471093359183854 1107 34-35 0.5658086115194436 1107 36-37 0.5671368089369722 1107 38-39 0.6566010292185354 1107 40-41 0.6281991112617291 1107 42-43 0.7212914779997419 1107 44-45 0.8995526640628455 1107 46-47 0.713838391087819 1107 48-49 0.8129759997451131 1107 50-51 0.7374228649194805 1107 52-53 0.7695425644161773 1107 54-55 0.9343892528604272 1107 56-57 1.0324046228903896 1107 58-59 1.0387258883205917 1107 60-61 1.0189401803367417 1107 62-63 1.1865545587619764 1107 64-65 1.2522603732153712 1107 66-67 1.4198474491457844 1107 68-69 1.5108165743473982 1107 70-71 1.7264870384028228 1107 72-73 1.88339321806005 1107 74-75 2.046053882273938 1107 76-77 2.0856672211610032 1107 78-79 2.201920078221338 1107 80-81 2.3095994190741287 1107 82-83 2.4487514379552735 1107 84-85 2.708832332104027 1107 86-87 2.710089127839492 1107 88-89 2.939712144072459 1107 90-91 3.29943634264637 1107 92-93 3.2678261295597224 1107 94-95 3.4778578908483837 1107 96-97 3.5501430629003075 1107 98-99 3.53144880583228 1107 100-101 3.576200550324508 1108 1 0.17211562750234322 1108 2 0.11112230580904736 1108 3 0.05477486154648403 1108 4 0.039890077746719044 1108 5 0.10398588571437983 1108 6 0.06910920222691885 1108 7 -1.1157517150195417E-5 1108 8 0.1536108230233637 1108 9 0.09279767386087912 1108 10-11 0.04477302384274395 1108 12-13 0.09254140730060101 1108 14-15 0.31121133764830944 1108 16-17 0.30968721797410836 1108 18-19 0.24981727120115949 1108 20-21 0.15655004680021278 1108 22-23 0.13920697252807912 1108 24-25 0.10774737279539437 1108 26-27 0.12899850668400603 1108 28-29 0.22977803556739929 1108 30-31 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4077.0 9 5273.0 10 6468.0 11 8051.0 12 10067.0 13 12202.0 14 14390.0 15 17350.0 16 20862.0 17 24932.0 18 24421.0 19 21197.0 20 21569.0 21 23352.0 22 25736.0 23 29027.0 24 32181.0 25 36131.0 26 41642.0 27 47303.0 28 54450.0 29 64024.0 30 75056.0 31 89353.0 32 109631.0 33 136644.0 34 177464.0 35 236581.0 36 311462.0 37 345597.0 38 176186.0 39 9353.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.61352647432725 18.256993645476967 14.731541553879248 23.397938326316535 2 15.585361369333494 19.252463015529216 40.869196722855264 24.292978892282026 3 19.499575055893047 23.35766390621181 29.33154834383921 27.811212694055943 4 12.974610874697706 16.377797791189156 36.625813593013326 34.02177774109981 5 14.131722611698278 36.78429008063029 34.08795534267089 14.996031965000542 6 32.8766078555227 35.487713184415284 17.152462746561774 14.483216213500244 7 29.126734767279057 32.32405224056635 20.023651942428106 18.525561049726484 8 26.360024018163397 35.053693330744366 19.18058421177957 19.40569843931266 9 26.252806799371974 17.11036557118339 19.926022029254845 36.710805600189786 10-11 25.214642897185787 26.337302388160104 26.865478192622493 21.58257652203162 12-13 26.179652281855475 23.513780286763428 27.348003365245766 22.95856406613533 14-15 22.97150618797535 25.125554341560274 25.667054034050512 26.235885436413863 16-17 22.43687464615833 28.23262462233533 26.479812106570105 22.85068862493624 18-19 22.36272812869498 26.73047509428538 28.77278876419158 22.134008012828062 20-21 22.925831614993122 25.875546196721643 29.175656417173755 22.022965771111476 22-23 22.679195485678598 25.764460145955287 29.035168070571586 22.521176297794526 24-25 22.54623860799295 26.00039741384956 29.100315150079798 22.353048828077693 26-27 22.26606658986676 26.344841134309306 28.84008300130494 22.549009274518987 28-29 22.372862885404892 26.587951233414497 28.80166863503833 22.23751724614228 30-31 22.470683627139866 26.21310675518833 28.72356503597613 22.592644581695676 32-33 22.19696392134 26.1674552099917 28.781083929412294 22.854496939256002 34-35 22.827193784021663 26.317694347428787 28.610497872507413 22.244613996042133 36-37 22.839464510225255 25.9645675161945 28.608673287600737 22.587294685979504 38-39 22.433473203318588 26.31804313842331 28.61651568823812 22.631967970019982 40-41 22.82479449118915 26.1458904066595 28.57924295997252 22.45007214217883 42-43 22.569358663550233 26.121416276626704 28.38209374679139 22.927131313031676 44-45 22.581534573555345 26.447994803055668 28.448405913989944 22.52206470939904 46-47 22.846151845700778 26.593062525333323 27.943754640706214 22.617030988259685 48-49 22.815399032404148 26.52950091244558 27.90005246001605 22.75504759513422 50-51 22.86794883951454 26.840335429938573 27.78534331042154 22.50637242012535 52-53 22.963350348751817 26.644163753303037 27.730723605104806 22.661762292840343 54-55 22.74715305566384 26.743847975268388 27.784733101980695 22.72426586708708 56-57 22.727505041420798 26.861021769792004 27.669756227466035 22.741716961321163 58-59 22.79952828021106 26.56089060983075 27.734249873854633 22.905331236103553 60-61 22.731431012920275 26.619416885060726 27.927651658057556 22.72150044396144 62-63 22.551407617823042 26.71887006804734 28.034827075112105 22.694895239017516 64-65 22.544479135470148 26.647041628633065 27.847095069948946 22.96138416594784 66-67 22.551113864659605 26.741336745183148 27.72378272410326 22.98376666605399 68-69 22.68323190208803 26.50287481927306 27.90672808580747 22.907165192831442 70-71 22.666086514419295 26.52804435340149 27.633718592086165 23.172150540093046 72-73 22.731453408936794 26.622267005455065 27.55896386344302 23.087315722165123 74-75 22.701109360184528 26.92672036008814 27.30875361175308 23.063416667974256 76-77 22.877745993448574 26.649770314512438 27.43431425421815 23.03816943782084 78-79 22.847249090248024 26.665965794132667 27.478208318744272 23.008576796875037 80-81 22.930280550524895 26.741058408927724 27.259150962154273 23.069510078393108 82-83 22.969336424654866 26.802210490662485 27.212737870624025 23.015715214058623 84-85 23.015726424149445 26.836329792694897 27.179536953891304 22.968406829264357 86-87 23.076623080590274 26.685020350354684 27.09729462100196 23.141061948053082 88-89 23.039360422558737 26.714173137433594 26.94827534024072 23.298191099766953 90-91 23.142960788041623 26.69254251758637 26.9537821908619 23.2107145035101 92-93 23.199881819451416 26.38416613782067 27.01999183585816 23.39596020686976 94-95 23.05934035777479 26.374209795176757 27.109933196360014 23.45651665068844 96-97 23.32912304489495 26.08912629116633 27.020052899875395 23.56169776406333 98-99 23.21892188479046 26.21245086203708 26.751183402437533 23.817443850734925 100-101 24.166400863379952 25.107176150026632 26.771108465922598 23.955314520670818 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 546.0 1 371.5 2 293.5 3 384.0 4 322.5 5 307.5 6 536.5 7 1279.0 8 2228.0 9 2621.0 10 2268.5 11 1830.5 12 1783.0 13 1940.5 14 2104.5 15 2230.5 16 2379.5 17 2574.0 18 2830.0 19 3081.0 20 3484.0 21 4076.5 22 4891.5 23 6051.5 24 7510.0 25 9559.5 26 12299.5 27 15171.0 28 17679.0 29 20485.0 30 23786.0 31 27773.5 32 32710.0 33 35779.5 34 37847.0 35 41709.5 36 46303.5 37 50903.5 38 54492.5 39 57103.5 40 60173.0 41 64820.0 42 68417.0 43 70277.5 44 73046.0 45 77426.0 46 80440.5 47 82560.5 48 84125.5 49 84495.0 50 84651.5 51 83225.0 52 82719.5 53 83557.5 54 84205.5 55 79756.5 56 71881.5 57 64584.0 58 58535.0 59 51663.0 60 42604.0 61 35740.5 62 29859.0 63 23201.5 64 16921.0 65 12671.5 66 9604.5 67 7181.0 68 5460.0 69 4090.5 70 3219.5 71 2310.5 72 1647.0 73 1349.5 74 1006.0 75 713.5 76 546.5 77 351.0 78 200.5 79 127.0 80 81.0 81 50.0 82 30.5 83 21.0 84 17.0 85 10.0 86 5.5 87 9.0 88 7.5 89 3.5 90 6.0 91 6.0 92 3.0 93 3.0 94 2.0 95 1.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.45955062475486075 2 0.03967053286266599 3 0.052445789208270296 4 0.05643525522496778 5 0.0812237350815263 6 0.08153751330755869 7 0.046484002913654954 8 0.04016361293214546 9 0.045811621000728414 10-11 0.05042864346949068 12-13 0.045856446461590185 14-15 0.03875161091499972 16-17 0.03978259651482042 18-19 0.04820978315683308 20-21 0.05614388972936628 22-23 0.04852356138286547 24-25 0.06548999831904521 26-27 0.0569955734857399 28-29 0.04610298649632992 30-31 0.047649464896060964 32-33 0.04594609738331372 34-35 0.05098896173026279 36-37 0.04610298649632992 38-39 0.026424609178013113 40-41 0.024967781700005607 42-43 0.024564352552249674 44-45 0.014949291197400124 46-47 0.027388356586541155 48-49 0.027119403821370534 50-51 0.016338880484114977 52-53 0.01564408584075755 54-55 0.016563007788423824 56-57 0.015823387684204628 58-59 0.014052781980164734 60-61 0.017414691544797446 62-63 0.01654059505799294 64-65 0.017526755196951866 66-67 0.009771950467865748 68-69 0.013447638258530846 70-71 0.0190956463271138 72-73 0.01692161147531798 74-75 0.013895892867148539 76-77 0.00968229954614221 78-79 0.014433798397489773 80-81 0.017280215162212134 82-83 0.014769989353953045 84-85 0.013357987336807306 86-87 0.0035187986776489045 88-89 0.02924861321230459 90-91 0.03312601557684765 92-93 0.017459517005659213 94-95 0.0339328738723595 96-97 0.027971087577744157 98-99 0.024541939821818796 100-101 0.15018770661735867 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2230875.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.076615522396146 #Duplication Level Percentage of deduplicated Percentage of total 1 76.53617831835514 42.91889845111954 2 13.194103787803666 14.797613705425135 3 4.333988094372782 7.291061521403545 4 1.955531826022792 4.3863842539875755 5 1.0560998538558508 2.9611252728966657 6 0.6215748625115978 2.0913488750069473 7 0.4248310047337118 1.6676159440111928 8 0.29048631240899253 1.3031591404382183 9 0.22339592692757027 1.1274558753227995 >10 1.2452877865124474 12.7973044423172 >50 0.08135795598976975 3.1067398246832263 >100 0.03395691107879353 3.328744135342672 >500 0.0021649676131173007 0.7753672405861913 >1k 8.820238423810141E-4 0.7680380327332065 >5k 1.6036797134200256E-4 0.6791432847258562 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8392 0.3761752675519695 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6712 0.30086849330419674 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4282 0.19194262341009694 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 4.482546086176949E-5 0.0 9 0.0 0.0 0.0 8.965092172353898E-5 0.0 10-11 0.0 0.0 0.0 1.3447638258530845E-4 0.0 12-13 0.0 0.0 0.0 2.2412730430884743E-4 0.0 14-15 0.0 0.0 0.0 2.2412730430884743E-4 0.0 16-17 0.0 0.0 0.0 2.4654003473973215E-4 0.0 18-19 0.0 0.0 0.0 3.586036868941559E-4 0.0 20-21 0.0 0.0 0.0 4.258418781868101E-4 0.0 22-23 0.0 0.0 0.0 4.4825460861769485E-4 0.0 24-25 0.0 0.0 0.0 6.275564520647728E-4 0.0 26-27 0.0 0.0 0.0 0.0012551129041295456 0.0 28-29 0.0 0.0 0.0 0.002958480416876786 0.0 30-31 0.0 0.0 0.0 0.009771950467865748 0.0 32-33 0.0 0.0 0.0 0.026110830951980725 0.0 34-35 0.0 0.0 0.0 0.05036140527819802 0.0 36-37 0.0 0.0 0.0 0.09182495657533479 0.0 38-39 0.0 0.0 0.0 0.14991875385218806 0.0 40-41 0.0 0.0 0.0 0.2104779514764386 0.0 42-43 0.0 0.0 0.0 0.27271810388300555 0.0 44-45 0.0 0.0 0.0 0.3545245699557349 0.0 46-47 0.0 0.0 0.0 0.44708914663528887 0.0 48-49 0.0 0.0 0.0 0.5440017930184344 0.0 50-51 0.0 0.0 0.0 0.6446125399226761 0.0 52-53 0.0 0.0 0.0 0.7436768084271866 0.0 54-55 0.0 0.0 0.0 0.8499355634000112 0.0 56-57 0.0 0.0 0.0 0.9691712892923181 0.0 58-59 0.0 0.0 0.0 1.0981565529220596 0.0 60-61 0.0 0.0 0.0 1.2356138286546758 0.0 62-63 0.0 0.0 0.0 1.3689023365271473 0.0 64-65 4.482546086176949E-5 0.0 0.0 1.5096542836331035 0.0 66-67 4.482546086176949E-5 0.0 0.0 1.6731551521264079 0.0 68-69 4.482546086176949E-5 0.0 0.0 1.848310640443772 0.0 70-71 4.482546086176949E-5 0.0 0.0 2.0268728637866307 0.0 72-73 4.482546086176949E-5 0.0 0.0 2.2137950355802096 0.0 74-75 4.482546086176949E-5 0.0 0.0 2.404348069703592 0.0 76-77 4.482546086176949E-5 0.0 0.0 2.6032834650081247 0.0 78-79 4.482546086176949E-5 0.0 0.0 2.8230178741525185 0.0 80-81 4.482546086176949E-5 0.0 0.0 3.051269120860649 0.0 82-83 4.482546086176949E-5 0.0 0.0 3.2851011374460697 0.0 84-85 4.482546086176949E-5 0.0 0.0 3.527674118899535 0.0 86-87 4.482546086176949E-5 0.0 0.0 3.7780467305429486 0.0 88-89 4.482546086176949E-5 0.0 0.0 4.052199249173531 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4100 0.0 51.57684 1 GTATCAA 7510 0.0 43.66683 1 ATCAACG 9305 0.0 34.682728 3 TATCAAC 9425 0.0 34.543713 2 TCAACGC 9365 0.0 34.460518 4 CAACGCA 9530 0.0 33.86388 5 AACGCAG 9875 0.0 32.76969 6 TGGTATC 1825 0.0 29.94986 2 ACGCAGA 11285 0.0 28.41564 7 CGCAGAG 11365 0.0 28.132017 8 GTGGTAT 2320 0.0 25.101904 1 GCAGAGT 12380 0.0 25.057516 9 GTACATG 7215 0.0 22.626877 1 GAGTACT 7710 0.0 22.583406 12-13 TACATGG 7195 0.0 22.195673 2 CAGAGTA 11910 0.0 21.419916 10-11 GTACTTT 8125 0.0 21.254976 14-15 AGAGTAC 11160 0.0 21.199244 10-11 TAAGGTG 1055 0.0 21.17411 5 ACATGGG 7535 0.0 20.878752 3 >>END_MODULE