FastQCFastQC Report
Thu 26 May 2016
SRR938282_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938282_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2230875
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81920.36721017537961564No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71490.32045721970079005No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44730.2005042864346949No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA43200.052.556471
GTATCAA74600.040.9405941
ATCAACG93300.032.4280173
TCAACGC95000.032.0477074
TATCAAC94900.031.9327622
CAACGCA96550.031.6316095
AACGCAG100100.030.509816
TGGTATC18500.029.269492
ACGCAGA113800.026.6692877
CGCAGAG114200.026.5758768
GTGGTAT22000.026.3402061
GCAGAGT127700.023.3572439
TACATGG71750.022.9053122
GAGTACT77050.022.71525812-13
GTACATG73850.022.446931
GTACTTT79500.021.8658714-15
CAGAGTA121100.021.27690310-11
AGAGTAC113200.020.89468210-11
ACATGGG76100.020.5964263
AGTACTT80950.020.15406812-13
Produced by FastQC (version 0.11.5)