##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938282_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2230875 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.465961113912705 33.0 31.0 34.0 31.0 34.0 2 32.70434874208551 34.0 31.0 34.0 31.0 34.0 3 32.666498571188434 34.0 31.0 34.0 31.0 34.0 4 36.196860424721244 37.0 35.0 37.0 35.0 37.0 5 36.15594374404662 37.0 35.0 37.0 35.0 37.0 6 36.21361035468146 37.0 37.0 37.0 35.0 37.0 7 36.17455706841486 37.0 37.0 37.0 35.0 37.0 8 36.17000683588278 37.0 37.0 37.0 35.0 37.0 9 37.881395864851235 39.0 38.0 39.0 35.0 39.0 10-11 37.84142455314618 39.0 38.0 39.0 35.0 39.0 12-13 37.779472628452964 39.0 38.0 39.0 35.0 39.0 14-15 39.05668314002354 40.0 38.0 41.0 36.0 41.0 16-17 38.98462665994285 40.0 38.0 41.0 36.0 41.0 18-19 38.97161315627277 40.0 38.0 41.0 35.5 41.0 20-21 38.97705451896677 40.0 38.5 41.0 35.5 41.0 22-23 39.0069230683028 40.0 38.5 41.0 35.0 41.0 24-25 38.95440623073905 40.0 38.0 41.0 35.0 41.0 26-27 38.78331125679386 40.0 38.0 41.0 35.0 41.0 28-29 38.7069286714854 40.0 38.0 41.0 34.5 41.0 30-31 38.50147430940774 40.0 38.0 41.0 34.0 41.0 32-33 38.31237137894324 40.0 38.0 41.0 34.0 41.0 34-35 38.11090446573654 40.0 38.0 41.0 33.5 41.0 36-37 38.03852053566426 40.0 38.0 41.0 33.0 41.0 38-39 37.99895265310697 40.0 38.0 41.0 33.0 41.0 40-41 37.8814355353841 40.0 37.5 41.0 33.0 41.0 42-43 37.69788513475655 40.0 37.0 41.0 33.0 41.0 44-45 37.45050170897069 40.0 37.0 41.0 32.5 41.0 46-47 37.10928178405334 40.0 37.0 41.0 31.5 41.0 48-49 36.752048411497725 40.0 36.0 41.0 31.0 41.0 50-51 36.45066464952093 39.0 36.0 41.0 30.0 41.0 52-53 35.988334397938026 39.0 35.0 40.0 29.0 41.0 54-55 35.67340886423489 39.0 35.0 40.0 28.0 41.0 56-57 35.39095377374349 38.0 35.0 40.0 28.0 41.0 58-59 35.0065021572253 38.0 34.0 40.0 27.0 41.0 60-61 34.58245015969071 37.5 34.0 40.0 26.0 41.0 62-63 34.13866352888441 37.0 33.5 40.0 26.0 41.0 64-65 33.69042169552306 36.5 33.0 39.0 24.5 41.0 66-67 33.160875217123326 36.0 32.0 39.0 23.0 40.0 68-69 32.611503109766346 35.0 31.5 38.5 22.0 40.0 70-71 32.01105956183112 35.0 31.0 38.0 20.5 40.0 72-73 31.522449263181485 35.0 31.0 37.0 18.5 39.0 74-75 30.955113576511458 34.0 30.0 36.5 17.5 39.0 76-77 29.750372163388803 33.5 28.5 35.0 16.0 37.5 78-79 30.448317364262902 34.0 30.0 36.0 16.5 37.5 80-81 30.246154311649015 34.0 30.0 35.0 11.5 37.0 82-83 29.77044679778114 34.0 29.5 35.0 7.0 37.0 84-85 29.238077660110942 34.0 29.0 35.0 3.5 36.0 86-87 28.665141480360845 34.0 29.0 35.0 2.0 36.0 88-89 28.014992323639827 33.0 27.0 35.0 2.0 35.0 90-91 27.45855034459573 33.0 26.0 35.0 2.0 35.0 92-93 26.784932817840534 32.5 25.0 35.0 2.0 35.0 94-95 25.997631198520757 32.0 24.0 34.0 2.0 35.0 96-97 25.129601837843893 32.0 19.5 34.0 2.0 35.0 98-99 24.05908802599877 31.0 8.5 34.0 2.0 35.0 100-101 20.552175715806577 27.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.09028866406325875 1101 2 0.06323351042964731 1101 3 0.21124513970330128 1101 4 0.09987212227589737 1101 5 0.005056641511487214 1101 6 0.08476019180781691 1101 7 0.13267659918788866 1101 8 0.18064942762601532 1101 9 0.19988091445752332 1101 10-11 0.21364694479296986 1101 12-13 0.23752703394321628 1101 14-15 0.3781448579958848 1101 16-17 0.3389574517389988 1101 18-19 0.3107522058029133 1101 20-21 0.3173245685227428 1101 22-23 0.20974533461536993 1101 24-25 0.1806673315723728 1101 26-27 0.2906445542060183 1101 28-29 0.2757317154779386 1101 30-31 0.31596533085915723 1101 32-33 0.3058335013905733 1101 34-35 0.3481801609135857 1101 36-37 0.42499524550122203 1101 38-39 0.4855666817788489 1101 40-41 0.45570510280607124 1101 42-43 0.5534677782454125 1101 44-45 0.6425141898463167 1101 46-47 0.6329336396363061 1101 48-49 0.7616051797589733 1101 50-51 0.8292410351218038 1101 52-53 0.8070668264900576 1101 54-55 0.8894453114347627 1101 56-57 0.9076275170830428 1101 58-59 0.8138007236914007 1101 60-61 0.8474945287677471 1101 62-63 0.7975639189660555 1101 64-65 0.7879707987825526 1101 66-67 0.7843260030851695 1101 68-69 0.7356099717664577 1101 70-71 0.841910337398474 1101 72-73 0.9676753196783778 1101 74-75 1.1335803055643154 1101 76-77 1.325579531180157 1101 78-79 1.1071887885143816 1101 80-81 1.377595350673161 1101 82-83 1.4093784733335148 1101 84-85 1.6456090370715906 1101 86-87 1.7148933704683458 1101 88-89 1.7988789050478502 1101 90-91 1.8226361972733507 1101 92-93 2.1466497409300658 1101 94-95 2.2707809222480684 1101 96-97 2.3627239502295154 1101 98-99 2.6964846325375333 1101 100-101 2.779310410532471 1102 1 0.11882455406973946 1102 2 0.1593805941004547 1102 3 0.21309012391933635 1102 4 0.08645043775366901 1102 5 0.06623442238423394 1102 6 0.1001095752146739 1102 7 0.13668021270113684 1102 8 0.14201206460542437 1102 9 0.15752407398796464 1102 10-11 0.1659685331649854 1102 12-13 0.10269530575882158 1102 14-15 0.22426930690941305 1102 16-17 0.19871864331310718 1102 18-19 0.2938537107851289 1102 20-21 0.3001033745208517 1102 22-23 0.2591348221175167 1102 24-25 0.2426245305317707 1102 26-27 0.3330260972995873 1102 28-29 0.15586564170405381 1102 30-31 0.24413535376665152 1102 32-33 0.23574321497650175 1102 34-35 0.23949811632323303 1102 36-37 0.3099002432332725 1102 38-39 0.32722297371603304 1102 40-41 0.2674626129067619 1102 42-43 0.3164433065518182 1102 44-45 0.3218706652535772 1102 46-47 0.32991269874130325 1102 48-49 0.3157267287409198 1102 50-51 0.43547271354326966 1102 52-53 0.28580032631088415 1102 54-55 0.4811577711029784 1102 56-57 0.6452435104336729 1102 58-59 0.6627220461692929 1102 60-61 0.605617388665415 1102 62-63 0.46460965727159476 1102 64-65 0.6643901657291877 1102 66-67 0.5776033795726079 1102 68-69 0.7777551073950377 1102 70-71 0.8243360411829457 1102 72-73 0.7322915046140679 1102 74-75 0.8822189227181312 1102 76-77 0.9435506566071155 1102 78-79 1.2776074968734115 1102 80-81 1.2917583151132312 1102 82-83 1.3522980865239553 1102 84-85 1.346643015139687 1102 86-87 1.4262532689215632 1102 88-89 1.5515221417041296 1102 90-91 1.7823937225756588 1102 92-93 1.9352168523706403 1102 94-95 1.9383909087253386 1102 96-97 2.276207871951037 1102 98-99 2.4450996648331405 1102 100-101 2.5970366781850664 1103 1 0.2995332412736289 1103 2 0.32052444482796005 1103 3 0.41331018564410016 1103 4 0.2722648816625579 1103 5 0.1874276372503516 1103 6 0.1105408144628015 1103 7 0.21908272020680641 1103 8 0.22074752981120582 1103 9 0.23669227313738617 1103 10-11 0.33831224371580504 1103 12-13 0.2912476455730868 1103 14-15 0.43641698282583974 1103 16-17 0.4164385384940843 1103 18-19 0.4899383083117712 1103 20-21 0.4756116969518658 1103 22-23 0.46475440139712276 1103 24-25 0.5596260761700904 1103 26-27 0.543813432358256 1103 28-29 0.5739515693417587 1103 30-31 0.5762545968797212 1103 32-33 0.7051558388276931 1103 34-35 0.5799292939921372 1103 36-37 0.6788269231002815 1103 38-39 0.7273286100616474 1103 40-41 0.7035165159933925 1103 42-43 0.6722632701976892 1103 44-45 0.7594360581821533 1103 46-47 0.8388265701314239 1103 48-49 0.8205083548464813 1103 50-51 0.699785682629944 1103 52-53 0.8169703258126404 1103 54-55 0.950911179932433 1103 56-57 0.9876228305655417 1103 58-59 0.9843360150225209 1103 60-61 1.011455490189391 1103 62-63 1.1816300685423897 1103 64-65 1.1251318761870763 1103 66-67 1.1676835557115623 1103 68-69 1.226204906618804 1103 70-71 1.5269986984828492 1103 72-73 1.3684596651365268 1103 74-75 1.3576148070992389 1103 76-77 0.9175615684441496 1103 78-79 1.3401863952436344 1103 80-81 1.576712217472103 1103 82-83 1.5969280990021453 1103 84-85 1.6837781652838082 1103 86-87 1.7849971256950212 1103 88-89 2.091125134901869 1103 90-91 2.0520524673561162 1103 92-93 2.338425661333442 1103 94-95 2.432712745219085 1103 96-97 2.916602745873192 1103 98-99 3.3889903628447478 1103 100-101 3.686462435203559 1104 1 0.10810350419709636 1104 2 0.10157486535983651 1104 3 0.20900575041181924 1104 4 0.09536161392639286 1104 5 0.028166691528582533 1104 6 0.04163679494919137 1104 7 0.1264205468874664 1104 8 0.05944822815948214 1104 9 0.09015551728118965 1104 10-11 0.04921688767328902 1104 12-13 0.06827852702195258 1104 14-15 0.11405190930597797 1104 16-17 0.1390220909685027 1104 18-19 0.04179961963371426 1104 20-21 0.07434513530993314 1104 22-23 0.15747390029364539 1104 24-25 0.06557675630966742 1104 26-27 0.19708587986753656 1104 28-29 0.12979065433285086 1104 30-31 0.07539624122480859 1104 32-33 0.12557120605519145 1104 34-35 0.04741797531031722 1104 36-37 0.03581450603901004 1104 38-39 0.16683497342991416 1104 40-41 0.21949255079518792 1104 42-43 0.27010804750909045 1104 44-45 0.3249041084045601 1104 46-47 0.3131558659716447 1104 48-49 0.22312388842256325 1104 50-51 0.0547602490209087 1104 52-53 0.2096596975844207 1104 54-55 0.3817079265085539 1104 56-57 0.4516811452699727 1104 58-59 0.37227075096069484 1104 60-61 0.3874682378086405 1104 62-63 0.3264627191130671 1104 64-65 0.4419486436764686 1104 66-67 0.4948851405562138 1104 68-69 0.594165588046998 1104 70-71 0.6769626376133289 1104 72-73 0.6003679829224282 1104 74-75 0.5495109670750296 1104 76-77 0.6859712026045912 1104 78-79 0.7796103737864009 1104 80-81 0.7615728152166703 1104 82-83 0.9363842674736347 1104 84-85 1.2557441753676102 1104 86-87 1.1276249189480723 1104 88-89 1.2519983783988131 1104 90-91 1.1655038847571184 1104 92-93 1.6195602493609265 1104 94-95 1.7750258971988409 1104 96-97 1.9480389871489265 1104 98-99 2.0094797618149762 1104 100-101 2.283770883480912 1105 1 -0.014399432766630582 1105 2 -0.03514617698726141 1105 3 0.048585719528162485 1105 4 0.024880555272844163 1105 5 -0.036087047420316765 1105 6 0.004219895477788782 1105 7 -0.014771155808283254 1105 8 -0.06616846063788984 1105 9 -0.04305901297202297 1105 10-11 -0.09517447741420426 1105 12-13 -0.07151754302072533 1105 14-15 0.019833413921780618 1105 16-17 0.005703069929431592 1105 18-19 0.011337888096299764 1105 20-21 0.004575460228444683 1105 22-23 0.040339821322895375 1105 24-25 -0.0024105966259924116 1105 26-27 -0.007912256590806521 1105 28-29 -0.057127908712807596 1105 30-31 -0.06577320199015446 1105 32-33 0.08540967580970715 1105 34-35 0.10017312874804674 1105 36-37 0.1390779850744437 1105 38-39 0.19857221000920333 1105 40-41 0.15645802431688338 1105 42-43 0.11608384611854916 1105 44-45 0.28570028772104195 1105 46-47 0.251795767544273 1105 48-49 0.2805337568414359 1105 50-51 0.3552638835710056 1105 52-53 0.500098735489189 1105 54-55 0.5677085159248918 1105 56-57 0.4922648697998824 1105 58-59 0.4633420133087185 1105 60-61 0.4879777039747708 1105 62-63 0.3839388140863633 1105 64-65 0.4232309958082112 1105 66-67 0.3454555903412171 1105 68-69 0.44207612185878276 1105 70-71 0.5900355938894535 1105 72-73 0.6753358906379319 1105 74-75 0.4220575031870837 1105 76-77 0.4586248233758923 1105 78-79 0.7067306261242869 1105 80-81 0.8993297512524911 1105 82-83 0.8131865955577773 1105 84-85 1.0346939178750851 1105 86-87 1.1340435598099319 1105 88-89 1.2153997447998002 1105 90-91 1.3267568985252503 1105 92-93 1.299693717943601 1105 94-95 1.49589982649022 1105 96-97 1.6190677762000618 1105 98-99 1.802354465221189 1105 100-101 2.2483237640968774 1106 1 0.02266643144883318 1106 2 0.028166711380301024 1106 3 0.10830420386008655 1106 4 0.027212816113333815 1106 5 0.017037341651580107 1106 6 0.024042194193533817 1106 7 0.08977551955526053 1106 8 0.06251606385679054 1106 9 0.04122047098900339 1106 10-11 0.08016348572638776 1106 12-13 0.04580138930991495 1106 14-15 0.05993715522262022 1106 16-17 0.12318707845821564 1106 18-19 0.05978999041979449 1106 20-21 0.07878191601415807 1106 22-23 0.050927627791836017 1106 24-25 0.07279042726666063 1106 26-27 0.021013350409077702 1106 28-29 0.04763399604910035 1106 30-31 0.01929119325725992 1106 32-33 0.141068674243904 1106 34-35 -0.007926293348234026 1106 36-37 0.07054980336090466 1106 38-39 0.12164907686843662 1106 40-41 0.1357005374215774 1106 42-43 0.1605234183638231 1106 44-45 0.16400624234015737 1106 46-47 -0.19263786670826022 1106 48-49 0.054459848016115586 1106 50-51 0.18508073760418142 1106 52-53 0.1989693292701915 1106 54-55 0.23620737308966966 1106 56-57 0.33006567480899207 1106 58-59 0.3772997676877665 1106 60-61 0.3340716923509248 1106 62-63 0.39063221085572764 1106 64-65 0.4958086145086895 1106 66-67 0.5876529765281688 1106 68-69 0.28217164132352224 1106 70-71 0.4987789684598596 1106 72-73 0.4194811211508096 1106 74-75 0.5196924463559256 1106 76-77 0.6075896942108443 1106 78-79 0.799855892192042 1106 80-81 0.9166171665447429 1106 82-83 0.7690756226953184 1106 84-85 0.953279287026394 1106 86-87 1.1455948741161066 1106 88-89 1.2758585597956973 1106 90-91 1.2025898161990902 1106 92-93 1.1849678613653118 1106 94-95 1.6881445339116148 1106 96-97 1.8646615115263643 1106 98-99 2.1547345954185353 1106 100-101 2.3914265571459588 1107 1 0.021428382064421214 1107 2 0.04349351264836088 1107 3 0.12384610057675616 1107 4 0.08638282280380594 1107 5 0.06367928901572384 1107 6 0.07999452303945986 1107 7 -0.01135935904711971 1107 8 0.0402624388106716 1107 9 -0.005405219160998342 1107 10-11 0.03693805444125786 1107 12-13 0.02148559917804249 1107 14-15 0.05393073987581687 1107 16-17 0.010311364860307037 1107 18-19 0.0853131913401981 1107 20-21 0.08650596856247006 1107 22-23 0.1068518191517498 1107 24-25 0.11265811855467689 1107 26-27 0.1373449725664102 1107 28-29 0.022749203422371522 1107 30-31 0.047881380152695385 1107 32-33 0.09731425822179318 1107 34-35 0.08759984637627838 1107 36-37 0.09002344478928848 1107 38-39 0.22769238358579003 1107 40-41 0.20117584208802697 1107 42-43 0.1683431383645697 1107 44-45 0.032101459592816184 1107 46-47 0.1716863021416586 1107 48-49 0.3439608140698027 1107 50-51 0.3865807782472146 1107 52-53 0.26247188352864725 1107 54-55 0.34553463706306786 1107 56-57 0.36218805411481014 1107 58-59 0.4334353028314837 1107 60-61 0.3912526914192611 1107 62-63 0.5131953482479474 1107 64-65 0.5399925961505403 1107 66-67 0.675900886538237 1107 68-69 0.7785681672819109 1107 70-71 0.7541316562150513 1107 72-73 0.6778338901920762 1107 74-75 0.7096592441224345 1107 76-77 0.698259929127591 1107 78-79 0.8525992970779583 1107 80-81 1.029183367796545 1107 82-83 1.0800361114121877 1107 84-85 1.2081098680967415 1107 86-87 1.230004831034492 1107 88-89 1.4189501817029218 1107 90-91 1.5287751859569205 1107 92-93 1.6591520305904055 1107 94-95 1.7068772215417525 1107 96-97 1.6534440337706116 1107 98-99 1.8372999838096327 1107 100-101 2.5166533765470653 1108 1 0.08154186015866571 1108 2 -0.007840156626805594 1108 3 0.10870815339666962 1108 4 0.06539166974434352 1108 5 -7.727674666924145E-4 1108 6 -0.022543480735208732 1108 7 -5.275290960113921E-4 1108 8 -0.00844238643016837 1108 9 0.011900827537182579 1108 10-11 -0.01514698019956029 1108 12-13 -0.024023926302696452 1108 14-15 0.04665136741289899 1108 16-17 0.03836027074078885 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33.0 6 65.0 7 192.0 8 491.0 9 910.0 10 1488.0 11 2516.0 12 3858.0 13 5791.0 14 8114.0 15 10982.0 16 14316.0 17 19325.0 18 19705.0 19 15491.0 20 14026.0 21 14665.0 22 15501.0 23 17128.0 24 19346.0 25 22601.0 26 26470.0 27 31943.0 28 38803.0 29 47125.0 30 57030.0 31 70613.0 32 88177.0 33 115491.0 34 158878.0 35 231563.0 36 351844.0 37 484290.0 38 303950.0 39 18122.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.7618311200762 18.72323639827422 15.138230514932483 23.376701966717096 2 15.815696852622843 19.559805635215906 40.39098899827898 24.23350851388227 3 19.799250562929537 23.295577568171293 29.440441539380863 27.464730329518304 4 13.26843201364129 16.718290678899837 36.26336583778376 33.74991146967511 5 14.097946497130492 36.82772334213856 34.173244441296696 14.90108571943425 6 32.96210314267387 35.657821541451746 16.91231810355686 14.467757212317519 7 29.160844959937243 32.25904633832016 19.966425729814535 18.613682971928057 8 26.31139185807894 35.2704366091496 19.041909135164065 19.376262397607395 9 25.93941838964532 17.05532582506864 19.88627780579369 37.11897797949235 10-11 25.25287163108646 26.337490894828264 26.83429147755925 21.57534599652603 12-13 26.06941222614445 23.472112960161372 27.405121308903457 23.05335350479072 14-15 22.826872863786633 25.146388748809322 25.607530677424776 26.41920770997927 16-17 22.342342059659128 28.277347801803238 26.533143512363317 22.847166626174314 18-19 22.28322967445509 26.749526531069645 28.804460133355747 22.162783661119516 20-21 22.804885975233933 25.877783380960384 29.21994733008349 22.097383313722194 22-23 22.664290917240994 25.730531742029473 29.053420742982013 22.55175659774752 24-25 22.53971662186656 26.01516337712933 29.082063648350587 22.363056352653523 26-27 22.268723795227785 26.294027884354783 28.893886518975044 22.543361801442394 28-29 22.26142216681784 26.589867049656178 28.863934504490512 22.28477627903547 30-31 22.44849382988323 26.213454057345697 28.77720318969229 22.560848923078787 32-33 22.143605435376568 26.142923166784186 28.83454448709495 22.878926910744298 34-35 22.780988554630355 26.280234690826177 28.660633066264246 22.278143688279222 36-37 22.76746498910543 25.97834265814049 28.674090200894476 22.580102151859606 38-39 22.3729933681868 26.30159674190852 28.694748060368937 22.630661829535743 40-41 22.775313656983176 26.117916867028267 28.64952355972233 22.457245916266224 42-43 22.517397301392343 26.13830907847375 28.45363024847267 22.890663371661233 44-45 22.550750833948623 26.404420588948618 28.511897637149303 22.53293093995346 46-47 22.823110627384064 26.555225159823053 27.99493819891417 22.626726013878713 48-49 22.753136051599586 26.52289767604171 28.01787463682899 22.706091635529717 50-51 22.821829999439682 26.760105339833025 27.861534151397993 22.5565305093293 52-53 22.919078836779292 26.59387011822715 27.798487140695915 22.68856390429764 54-55 22.709093965372332 26.74578360508769 27.772264246091783 22.7728581834482 56-57 22.67639379167367 26.82082142657029 27.70233652714742 22.800448254608618 58-59 22.724939765786967 26.583358547655067 27.781700005603184 22.910001680954785 60-61 22.681615957864068 26.708533647111558 27.866980444892697 22.742869950131674 62-63 22.475844679778113 26.862083263293552 27.97700453857791 22.685067518350426 64-65 22.420888664761584 26.752529836947385 27.817627612483893 23.008953885807138 66-67 22.457900345941656 26.82138226497091 27.655228291236483 23.065489097850946 68-69 22.57749218914594 26.625240377072622 27.829792861081103 22.967474572700336 70-71 22.5717711660223 26.59602174034852 27.602711940382136 23.229495153247047 72-73 22.588378999271587 26.719784837787863 27.50212360620833 23.18971255673222 74-75 22.601064604695466 27.033518238359388 27.190384938645153 23.175032218299997 76-77 22.623029080517735 26.710730094693787 27.501989129825745 23.16425169496274 78-79 22.567893763657757 26.75546590463383 27.58955566761921 23.087084664089204 80-81 22.67482073145422 26.83243450652234 27.34862302661158 23.14412173541186 82-83 22.64575267465196 26.887317889002897 27.37547240940789 23.091457026937253 84-85 22.645340888205524 26.908673608900884 27.34667987573909 23.099305627154507 86-87 22.721263012092884 26.79279156109254 27.26131095329935 23.22463447351523 88-89 22.607669148429977 26.89527218822582 27.087663195604772 23.40939546773943 90-91 22.63092600499598 26.863009446194567 27.19313876122499 23.312925787584458 92-93 22.690058217393496 26.649087516599522 27.186077133843973 23.47477713216301 94-95 22.49949291140574 26.613062826315716 27.283607646783935 23.60383661549461 96-97 22.714901904972155 26.399984759326227 27.24974197315176 23.635371362549854 98-99 22.538765110683176 26.478726704176665 27.073359654879347 23.90914853026081 100-101 22.7262610702329 26.08907019793452 27.193510349475652 23.991158382356932 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 507.0 1 330.0 2 257.0 3 370.5 4 310.5 5 285.0 6 543.0 7 1385.0 8 2337.5 9 2744.5 10 2427.0 11 1964.0 12 1949.0 13 2064.5 14 2172.5 15 2308.0 16 2466.5 17 2645.5 18 2873.0 19 3112.5 20 3469.0 21 4045.0 22 4904.5 23 6029.5 24 7552.5 25 9560.5 26 12156.5 27 15083.0 28 17721.5 29 20520.5 30 23922.0 31 27888.5 32 32566.5 33 35813.5 34 37973.0 35 41663.0 36 46453.0 37 50836.5 38 54090.5 39 56914.0 40 60130.5 41 64973.0 42 68666.5 43 70284.0 44 73511.0 45 78125.5 46 81112.5 47 83668.5 48 85136.5 49 85088.0 50 85271.0 51 84025.5 52 83162.0 53 84634.0 54 86132.5 55 81188.5 56 72114.5 57 64381.5 58 58424.0 59 51039.5 60 41567.0 61 34703.0 62 28944.5 63 22124.5 64 15830.0 65 11637.0 66 8605.0 67 6382.0 68 4752.5 69 3579.0 70 2770.5 71 1903.0 72 1390.0 73 1170.0 74 897.5 75 613.0 76 436.0 77 249.0 78 133.5 79 77.5 80 33.0 81 16.5 82 11.5 83 8.5 84 5.0 85 1.0 86 0.0 87 1.0 88 1.0 89 1.0 90 1.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.010040903233036365 3 0.006006611755477111 4 4.482546086176949E-5 5 1.7930184344707795E-4 6 1.7930184344707795E-4 7 0.0 8 4.482546086176949E-5 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 4.482546086176949E-5 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0017706057040398947 26-27 0.021717935787527315 28-29 2.0171457387796267E-4 30-31 6.723819129265422E-5 32-33 0.003675687790665098 34-35 0.016406118675407633 36-37 0.007911693842102314 38-39 0.02194206309183616 40-41 0.023869557908892253 42-43 0.019207709979268225 44-45 0.009951252311312825 46-47 0.00217403485179582 48-49 2.0171457387796267E-4 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 3.3619095646327115E-4 68-69 2.241273043088474E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 1.7930184344707795E-4 82-83 0.0010758110606824676 84-85 0.003810164173250406 86-87 7.84445565080966E-4 88-89 6.723819129265422E-5 90-91 0.003698100521095983 92-93 5.603182607721186E-4 94-95 1.120636521544237E-4 96-97 1.120636521544237E-4 98-99 1.3447638258530845E-4 100-101 0.0027567658429988235 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2230875.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.34901395710191 #Duplication Level Percentage of deduplicated Percentage of total 1 74.98865527814597 40.755594723362584 2 14.07170800629929 15.295669096692455 3 4.597813327564604 7.496598621358737 4 2.063278883474411 4.4854869134137765 5 1.1341048561280624 3.081874032726056 6 0.6756012935615151 2.2030958479926532 7 0.4687690002234241 1.783399305505971 8 0.32244469673902526 1.401964105877022 9 0.23837988335732738 1.1660140446911738 >10 1.306596984860856 13.094951411576625 >50 0.09170747944871871 3.3558963056243645 >100 0.037387141591131254 3.5321663771386005 >500 0.0025615866691803255 0.9103172626818504 >1k 8.263182803807018E-4 0.7480140269969503 >5k 1.6526365607614032E-4 0.6889579243612041 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8192 0.36721017537961564 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7149 0.32045721970079005 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4473 0.2005042864346949 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 4.482546086176949E-5 0.0 9 0.0 0.0 0.0 8.965092172353898E-5 0.0 10-11 0.0 0.0 0.0 8.965092172353898E-5 0.0 12-13 0.0 0.0 0.0 1.3447638258530845E-4 0.0 14-15 0.0 0.0 0.0 1.3447638258530845E-4 0.0 16-17 0.0 0.0 0.0 1.568891130161932E-4 0.0 18-19 0.0 0.0 0.0 3.361909564632711E-4 0.0 20-21 0.0 0.0 0.0 4.482546086176948E-4 0.0 22-23 0.0 0.0 0.0 4.930800694794643E-4 0.0 24-25 0.0 0.0 0.0 6.723819129265423E-4 0.0 26-27 0.0 0.0 0.0 0.001389589286714854 0.0 28-29 0.0 0.0 0.0 0.003115369529892979 0.0 30-31 0.0 0.0 0.0 0.009749537737434862 0.0 32-33 0.0 0.0 0.0 0.026066005491118954 0.0 34-35 0.0 0.0 0.0 0.05045105619992156 0.0 36-37 0.0 0.0 0.0 0.09258698940998487 0.0 38-39 0.0 0.0 0.0 0.15108421583459405 0.0 40-41 0.0 0.0 0.0 0.21180030257186083 0.0 42-43 0.0 0.0 0.0 0.27386115313498066 0.0 44-45 0.0 0.0 0.0 0.3556452064772791 0.0 46-47 0.0 0.0 0.0 0.4482097831568331 0.0 48-49 0.0 0.0 0.0 0.5455930968790272 0.0 50-51 0.0 0.0 0.0 0.6463383201658542 0.0 52-53 0.0 0.0 0.0 0.7451336359051941 0.0 54-55 0.0 0.0 0.0 0.8517734072953438 0.0 56-57 0.0 0.0 0.0 0.9722418333613493 0.0 58-59 0.0 0.0 0.0 1.10221325713005 0.0 60-61 0.0 0.0 0.0 1.2400963747408529 0.0 62-63 0.0 0.0 0.0 1.3738331372219421 0.0 64-65 0.0 0.0 0.0 1.5152126407799629 0.0 66-67 0.0 0.0 0.0 1.6796100184905027 0.0 68-69 0.0 0.0 0.0 1.8561326833641507 0.0 70-71 0.0 0.0 0.0 2.035367288619936 0.0 72-73 0.0 0.0 0.0 2.2230963187090267 0.0 74-75 0.0 0.0 0.0 2.415935451336359 0.0 76-77 0.0 0.0 0.0 2.6174707233708747 0.0 78-79 0.0 0.0 0.0 2.8411497730711046 0.0 80-81 0.0 0.0 0.0 3.0736594385611027 0.0 82-83 0.0 0.0 0.0 3.3132963523281225 0.0 84-85 0.0 0.0 0.0 3.5612035636241384 0.0 86-87 0.0 0.0 0.0 3.8179413907099233 0.0 88-89 0.0 0.0 0.0 4.102246876225696 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4320 0.0 52.55647 1 GTATCAA 7460 0.0 40.940594 1 ATCAACG 9330 0.0 32.428017 3 TCAACGC 9500 0.0 32.047707 4 TATCAAC 9490 0.0 31.932762 2 CAACGCA 9655 0.0 31.631609 5 AACGCAG 10010 0.0 30.50981 6 TGGTATC 1850 0.0 29.26949 2 ACGCAGA 11380 0.0 26.669287 7 CGCAGAG 11420 0.0 26.575876 8 GTGGTAT 2200 0.0 26.340206 1 GCAGAGT 12770 0.0 23.357243 9 TACATGG 7175 0.0 22.905312 2 GAGTACT 7705 0.0 22.715258 12-13 GTACATG 7385 0.0 22.44693 1 GTACTTT 7950 0.0 21.86587 14-15 CAGAGTA 12110 0.0 21.276903 10-11 AGAGTAC 11320 0.0 20.894682 10-11 ACATGGG 7610 0.0 20.596426 3 AGTACTT 8095 0.0 20.154068 12-13 >>END_MODULE