FastQCFastQC Report
Thu 26 May 2016
SRR938281_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938281_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2258738
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84940.3760506973363002No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69210.3064100395884782No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43460.19240832712780323No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA41400.052.8229061
GTATCAA72000.043.1060331
TATCAAC93350.033.0895922
ATCAACG93350.033.0895923
TCAACGC94350.032.638144
CAACGCA95850.032.0777975
AACGCAG99350.031.0433946
TGGTATC18750.027.6259732
ACGCAGA114050.026.9690117
CGCAGAG115000.026.580878
GTGGTAT21550.025.0373481
GCAGAGT126700.023.4503759
GAGTACT78700.022.04360812-13
GTACATG73350.021.9374891
TACATGG73950.021.334982
AGAGTAC113250.021.23949810-11
GTACTTT82000.020.88477314-15
CAGAGTA121100.020.8440610-11
AGTACTT82400.020.4192912-13
ACATGGG78500.019.0692373
Produced by FastQC (version 0.11.5)