##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938281_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2258738 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.856776217516153 33.0 31.0 34.0 30.0 34.0 2 32.042796906945384 34.0 31.0 34.0 30.0 34.0 3 32.114446651183094 34.0 31.0 34.0 30.0 34.0 4 35.63687377641851 37.0 35.0 37.0 33.0 37.0 5 35.65499761371173 37.0 35.0 37.0 33.0 37.0 6 35.67869934450122 37.0 35.0 37.0 35.0 37.0 7 35.618373180067806 37.0 35.0 37.0 33.0 37.0 8 35.60457122517087 37.0 35.0 37.0 33.0 37.0 9 37.26883374698615 39.0 37.0 39.0 34.0 39.0 10-11 37.18350556815355 39.0 37.0 39.0 34.0 39.0 12-13 37.08790196118363 39.0 37.0 39.0 33.0 39.0 14-15 38.33922836557405 40.0 38.0 41.0 33.5 41.0 16-17 38.25010957446149 40.0 38.0 41.0 33.0 41.0 18-19 38.18424491906542 40.0 38.0 41.0 33.0 41.0 20-21 38.0780652736174 40.0 38.0 41.0 33.0 41.0 22-23 37.92571493462279 40.0 38.0 41.0 33.0 41.0 24-25 37.881065223146734 40.0 38.0 41.0 33.0 41.0 26-27 37.89529330094947 40.0 38.0 41.0 33.0 41.0 28-29 37.808901032346384 40.0 38.0 41.0 33.0 41.0 30-31 37.63549513046666 40.0 37.5 41.0 32.0 41.0 32-33 37.423588304619656 40.0 37.0 41.0 32.0 41.0 34-35 37.220835926964526 40.0 37.0 41.0 31.0 41.0 36-37 37.00304218550359 40.0 36.5 41.0 31.0 41.0 38-39 36.76627413183822 39.5 36.0 41.0 30.0 41.0 40-41 36.54842017976409 39.0 36.0 41.0 30.0 41.0 42-43 36.26229846046775 39.0 35.0 41.0 30.0 41.0 44-45 36.048890132454495 39.0 35.0 41.0 29.0 41.0 46-47 35.91975806844353 39.0 35.0 41.0 28.0 41.0 48-49 35.681482093098005 39.0 35.0 41.0 27.0 41.0 50-51 35.04450449764426 38.5 34.5 40.5 26.5 40.5 52-53 35.13065614515716 38.5 35.0 40.0 26.5 41.0 54-55 35.27814093533646 39.0 35.0 40.5 26.5 41.0 56-57 35.06687451134218 39.0 35.0 40.0 26.0 41.0 58-59 34.80606427128777 38.0 34.5 40.0 26.0 41.0 60-61 34.439536148061435 38.0 34.0 40.0 24.5 41.0 62-63 34.0163932248893 37.5 34.0 40.0 23.0 41.0 64-65 33.59117613463802 37.0 33.0 40.0 22.0 41.0 66-67 33.155195733192606 36.0 33.0 39.0 21.5 41.0 68-69 32.66689983521772 36.0 32.0 39.0 19.5 40.5 70-71 32.17917947986885 35.0 32.0 38.5 18.0 40.0 72-73 31.680082639066594 35.0 31.0 37.5 14.0 40.0 74-75 31.148946225724277 35.0 31.0 37.0 9.0 39.0 76-77 30.597825644231424 35.0 30.5 36.5 7.0 39.0 78-79 30.02444174578902 34.0 30.0 36.0 2.0 38.5 80-81 29.489443220063592 34.0 29.0 35.5 2.0 37.0 82-83 28.95093167069399 34.0 29.0 35.0 2.0 37.0 84-85 28.45576866374055 34.0 28.5 35.0 2.0 36.0 86-87 27.940093096233383 34.0 27.0 35.0 2.0 36.0 88-89 27.388543071396505 33.0 26.0 35.0 2.0 35.5 90-91 26.77087293878263 33.0 25.0 35.0 2.0 35.0 92-93 26.149457130486137 32.0 24.0 35.0 2.0 35.0 94-95 25.535275007548464 32.0 21.5 34.5 2.0 35.0 96-97 24.686969006586864 32.0 15.0 34.0 2.0 35.0 98-99 23.600877126962047 31.0 2.0 34.0 2.0 35.0 100-101 20.720910304780812 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.47596073592152877 1101 2 0.3618648326449758 1101 3 0.4044117241663443 1101 4 0.25185789435324324 1101 5 0.2096801930109038 1101 6 0.2618257503725303 1101 7 0.28894065237324895 1101 8 0.24149518508111356 1101 9 0.2692621269015447 1101 10-11 0.41113683042110694 1101 12-13 0.23411563271844216 1101 14-15 0.37899291706415994 1101 16-17 0.5301870033243148 1101 18-19 0.4683873440033395 1101 20-21 0.5644177143215288 1101 22-23 0.6124090020371469 1101 24-25 0.5060844835674772 1101 26-27 0.47925847256035325 1101 28-29 0.6007111548534994 1101 30-31 0.5787158900741076 1101 32-33 0.5749556950539727 1101 34-35 0.6486532035154582 1101 36-37 0.7097162777946409 1101 38-39 0.6780964997827894 1101 40-41 0.7511773374127984 1101 42-43 0.8263381970268497 1101 44-45 0.8291669526983299 1101 46-47 0.6814678310097904 1101 48-49 0.7144030168679194 1101 50-51 0.6998420027895449 1101 52-53 0.7085450274527716 1101 54-55 0.592494672923408 1101 56-57 0.583125162466203 1101 58-59 0.7515837021923417 1101 60-61 0.6388424090975278 1101 62-63 0.5809874441195149 1101 64-65 0.7220651931652 1101 66-67 0.49285939891122865 1101 68-69 0.5918442349233786 1101 70-71 0.9000201721893006 1101 72-73 0.8499773232345547 1101 74-75 0.8633307030139079 1101 76-77 1.0054327371976797 1101 78-79 1.0722779134876639 1101 80-81 1.0556655135231487 1101 82-83 1.0668915330684996 1101 84-85 1.0411797307744344 1101 86-87 1.052560697678235 1101 88-89 0.9974397109207267 1101 90-91 1.140666540256678 1101 92-93 1.3074659062192922 1101 94-95 1.327305147558107 1101 96-97 1.29159732305164 1101 98-99 1.3614950867984632 1101 100-101 1.246149880891906 1102 1 0.3851630796122514 1102 2 0.2994076509702239 1102 3 0.23098190372128613 1102 4 0.20362791130146007 1102 5 0.23676240130281911 1102 6 0.2933698534422362 1102 7 0.28362344798962624 1102 8 0.3743819061769784 1102 9 0.3944495040091951 1102 10-11 0.39873150356000053 1102 12-13 0.3071569370077398 1102 14-15 0.32777135293785165 1102 16-17 0.3805292940454734 1102 18-19 0.3781738748350918 1102 20-21 0.4311348228944567 1102 22-23 0.5313796533919017 1102 24-25 0.4261009088939858 1102 26-27 0.4662721848821718 1102 28-29 0.4307192607143051 1102 30-31 0.3691767181624357 1102 32-33 0.39981525539724316 1102 34-35 0.4523000176095593 1102 36-37 0.47207760427215817 1102 38-39 0.6585444203502462 1102 40-41 0.6733214677616317 1102 42-43 0.7479527652367821 1102 44-45 0.4686181305179531 1102 46-47 0.5731201961611347 1102 48-49 0.6089106787197949 1102 50-51 0.7298344665417957 1102 52-53 0.5918253044449173 1102 54-55 0.6804929925648864 1102 56-57 0.5911831846762183 1102 58-59 0.6406799287506004 1102 60-61 0.5705534799945511 1102 62-63 0.574020253164079 1102 64-65 0.5089905264611474 1102 66-67 0.5600106594360028 1102 68-69 0.4361109126262974 1102 70-71 0.48368932134537346 1102 72-73 0.7898497699607105 1102 74-75 0.6391228823601338 1102 76-77 0.44850124718560025 1102 78-79 0.47223049765077363 1102 80-81 0.6286352824226782 1102 82-83 0.6273478376281432 1102 84-85 0.5579483826538549 1102 86-87 0.49851220625168935 1102 88-89 0.6925545446905055 1102 90-91 0.8065594454367933 1102 92-93 0.7646506960376342 1102 94-95 0.657223361358362 1102 96-97 0.7251990197865332 1102 98-99 1.0089392040862393 1102 100-101 1.0617563441310587 1103 1 0.22361878376317534 1103 2 0.3146778047357941 1103 3 0.18221042485366468 1103 4 0.18872124767926834 1103 5 0.12813131525638966 1103 6 0.1370697213937362 1103 7 0.162212073686284 1103 8 0.17815284358699301 1103 9 0.23948769565832606 1103 10-11 0.22394500155240848 1103 12-13 0.13486533732371697 1103 14-15 0.2384527794309932 1103 16-17 0.25645522852154556 1103 18-19 0.27535590280788824 1103 20-21 0.275350754507933 1103 22-23 0.2513738778441237 1103 24-25 0.4165777609518173 1103 26-27 0.16728559204401705 1103 28-29 0.45271765480981685 1103 30-31 0.42485849723262703 1103 32-33 0.38341066400465706 1103 34-35 0.4741647262424138 1103 36-37 0.5232973949253363 1103 38-39 0.39469643226399853 1103 40-41 0.4350348956583687 1103 42-43 0.3948656384123055 1103 44-45 0.3342724253680558 1103 46-47 0.4299964768762621 1103 48-49 0.3193564662216062 1103 50-51 0.26010729821395984 1103 52-53 0.34042265642342073 1103 54-55 0.3407383148328975 1103 56-57 0.2351376636219129 1103 58-59 0.35708531763888374 1103 60-61 0.2742707123654853 1103 62-63 0.2247490383095112 1103 64-65 0.2758363638274304 1103 66-67 0.3581853806692834 1103 68-69 0.26564893596186323 1103 70-71 0.5352142590357758 1103 72-73 0.5082806929822254 1103 74-75 0.3277063747029203 1103 76-77 0.25413643571778266 1103 78-79 0.2638226264522068 1103 80-81 0.3963269898945434 1103 82-83 0.5083700844001733 1103 84-85 0.5534625742996262 1103 86-87 0.34570751882383277 1103 88-89 0.37700942280377703 1103 90-91 0.5944955811420201 1103 92-93 0.5076025130266437 1103 94-95 0.5360499765599727 1103 96-97 0.6429077474954781 1103 98-99 0.5690864343836921 1103 100-101 0.6112353905612586 1104 1 0.2731542252730286 1104 2 0.32300897995721556 1104 3 0.12531197032740238 1104 4 0.2094237828299299 1104 5 0.16236003262076082 1104 6 0.2861218786424047 1104 7 0.23874808493768285 1104 8 0.2636190555228808 1104 9 0.22936143615100946 1104 10-11 0.41378388023730395 1104 12-13 0.18693075002775572 1104 14-15 0.24030433984551536 1104 16-17 0.34301958740294225 1104 18-19 0.3571845341133937 1104 20-21 0.22709205200465732 1104 22-23 0.2791107289662733 1104 24-25 0.30016183368748983 1104 26-27 0.22063053269926058 1104 28-29 0.35875183531828014 1104 30-31 0.3586348079369941 1104 32-33 0.3899283279846415 1104 34-35 0.38980941071316266 1104 36-37 0.4293864578797866 1104 38-39 0.33518284412737387 1104 40-41 0.38916323093732075 1104 42-43 0.47061128267843344 1104 44-45 0.44854704079251917 1104 46-47 0.355068192595148 1104 48-49 0.30864542478984447 1104 50-51 0.27466070849560253 1104 52-53 0.4012916716470727 1104 54-55 0.37516972870830045 1104 56-57 0.39596872927765503 1104 58-59 0.3572186889549869 1104 60-61 0.37180000624455545 1104 62-63 0.2925646173541736 1104 64-65 0.3792336967995311 1104 66-67 0.501312723880794 1104 68-69 0.5672236936225019 1104 70-71 0.5425117318535229 1104 72-73 0.5270389353096405 1104 74-75 0.4213381184706222 1104 76-77 0.40082336871537905 1104 78-79 0.5909387381852973 1104 80-81 0.6072509871693441 1104 82-83 0.6029151079269113 1104 84-85 0.6059261088486743 1104 86-87 0.5821392036252817 1104 88-89 0.7347023281270779 1104 90-91 0.8196788546458507 1104 92-93 0.7556227607646093 1104 94-95 0.9047211639730683 1104 96-97 0.7299190347527933 1104 98-99 0.8400062083994939 1104 100-101 0.8915194445711663 1105 1 0.3596529580892529 1105 2 0.27426080393387053 1105 3 0.247492486416796 1105 4 0.1623363855486133 1105 5 0.17563378271675845 1105 6 0.19100917608499657 1105 7 0.22040943967234483 1105 8 0.2826652117320805 1105 9 0.3217381737287752 1105 10-11 0.3187398168013118 1105 12-13 0.25416588941797613 1105 14-15 0.4137242000713073 1105 16-17 0.30256666520034514 1105 18-19 0.39234552405272183 1105 20-21 0.3455314959797775 1105 22-23 0.31186790967436195 1105 24-25 0.45509713051568923 1105 26-27 0.2837026853757081 1105 28-29 0.3568568195744817 1105 30-31 0.4807750045424797 1105 32-33 0.34989060046755327 1105 34-35 0.47695565524123396 1105 36-37 0.5480471790225536 1105 38-39 0.5783136520530334 1105 40-41 0.5777801766404096 1105 42-43 0.6095023048733523 1105 44-45 0.5960256330665317 1105 46-47 0.7293510817741691 1105 48-49 0.6797305948996666 1105 50-51 0.6625250238323659 1105 52-53 0.7140902278255581 1105 54-55 0.7137065154374298 1105 56-57 0.8429452479782142 1105 58-59 0.8043387258185177 1105 60-61 0.7145280993249585 1105 62-63 0.7163276731870596 1105 64-65 0.8075254946645174 1105 66-67 0.8025079085824345 1105 68-69 0.7960353248336176 1105 70-71 0.9189382735937883 1105 72-73 0.9265483815887308 1105 74-75 0.8017999685589459 1105 76-77 0.6622169828771156 1105 78-79 0.8465839459266356 1105 80-81 0.9472152756264514 1105 82-83 0.9966747078040328 1105 84-85 0.9478386923676041 1105 86-87 1.0794137985104655 1105 88-89 1.057120921169087 1105 90-91 1.0206479622412736 1105 92-93 1.2658715895796036 1105 94-95 1.5016636031098471 1105 96-97 1.3228688849947083 1105 98-99 1.3319480715436427 1105 100-101 1.316214648472556 1106 1 0.006524517995234902 1106 2 0.13174644851451234 1106 3 0.1123496016469332 1106 4 0.13499340259660642 1106 5 0.0952564508908651 1106 6 0.23274662810963065 1106 7 0.231932824873617 1106 8 0.18852936334911163 1106 9 0.14711193529492306 1106 10-11 -0.34755153870730027 1106 12-13 -0.12085214783174081 1106 14-15 -0.05864942761011349 1106 16-17 0.08044779042720052 1106 18-19 0.16140866558377098 1106 20-21 0.18111508468584958 1106 22-23 0.18223270840096717 1106 24-25 0.20777668482826783 1106 26-27 0.1725712849413199 1106 28-29 0.06969264713094248 1106 30-31 0.23326042598188934 1106 32-33 0.22373454292687 1106 34-35 -0.005174265022965585 1106 36-37 -0.23252967825631288 1106 38-39 -0.01175340188764551 1106 40-41 0.18251765097917172 1106 42-43 0.13592668974735744 1106 44-45 0.13756616295258084 1106 46-47 0.29915821336799553 1106 48-49 0.36792344842976377 1106 50-51 0.4558525430968885 1106 52-53 0.30542490992367277 1106 54-55 -0.0017753952465753287 1106 56-57 0.09569230251393179 1106 58-59 0.31955547033668097 1106 60-61 0.1624725966621483 1106 62-63 0.3742155298411305 1106 64-65 0.384882237992592 1106 66-67 0.14113105279117377 1106 68-69 -0.1433771814887166 1106 70-71 0.15954997809529203 1106 72-73 0.539903012513129 1106 74-75 0.5198340571013844 1106 76-77 0.5373354336819105 1106 78-79 0.5410398160286825 1106 80-81 0.6076441530772847 1106 82-83 0.6202382361132202 1106 84-85 0.3878031013408574 1106 86-87 0.5477355532931263 1106 88-89 0.42528796469650487 1106 90-91 0.5308386364253188 1106 92-93 0.6348936359334729 1106 94-95 0.6132323603619376 1106 96-97 0.7286333668607661 1106 98-99 0.810775687502705 1106 100-101 0.5315446371667321 1107 1 0.2295108862680202 1107 2 0.1695167473907091 1107 3 0.16546653510673792 1107 4 0.05637329566498295 1107 5 0.15283714532117187 1107 6 0.08707453626205108 1107 7 0.016151747090063395 1107 8 0.18280085819456815 1107 9 0.11313443988140648 1107 10-11 0.13541049931720295 1107 12-13 0.14499859806365123 1107 14-15 0.2741582687512505 1107 16-17 0.2828559848262415 1107 18-19 0.2735268084105442 1107 20-21 0.2490797218668348 1107 22-23 0.24892125800020182 1107 24-25 0.2922473054282406 1107 26-27 0.24565831470389554 1107 28-29 0.30021549737843856 1107 30-31 0.28246969291258495 1107 32-33 0.39664534809373464 1107 34-35 0.3535249598746333 1107 36-37 0.2893162766166171 1107 38-39 0.3201699560438911 1107 40-41 0.33704741801972915 1107 42-43 0.42204603300928767 1107 44-45 0.3695991141831172 1107 46-47 0.22278204486405428 1107 48-49 0.3034473079717088 1107 50-51 0.39603371591489633 1107 52-53 0.34518539452791686 1107 54-55 0.43270683670537124 1107 56-57 0.3126287098187248 1107 58-59 0.2567446657057175 1107 60-61 0.2172490602730619 1107 62-63 0.4558753100829023 1107 64-65 0.30313037079788074 1107 66-67 0.39273511640708847 1107 68-69 0.44811151887945044 1107 70-71 0.5353764830604817 1107 72-73 0.5171646805820913 1107 74-75 0.6459435217823319 1107 76-77 0.7581105539487218 1107 78-79 0.6252325680441508 1107 80-81 0.826709457107139 1107 82-83 0.7435847349333287 1107 84-85 0.8075566012702957 1107 86-87 0.7908768170012195 1107 88-89 0.7011033942182756 1107 90-91 0.8303605166783399 1107 92-93 0.8172494720669299 1107 94-95 0.8583807802355707 1107 96-97 0.6934907169358802 1107 98-99 0.5382359990084851 1107 100-101 0.5988612715895663 1108 1 0.2581285187815432 1108 2 0.2097479076454789 1108 3 0.1975698422022063 1108 4 0.20351124254099062 1108 5 0.18107337571194648 1108 6 0.20020427359352055 1108 7 0.1785522790502796 1108 8 0.20749660172228346 1108 9 0.25109624872182223 1108 10-11 0.297238840253101 1108 12-13 0.12217543179357904 1108 14-15 0.25485626450154086 1108 16-17 0.2733446486099993 1108 18-19 0.22391756551395758 1108 20-21 0.2982972853379593 1108 22-23 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2316 64-65 -0.9957808962118051 2316 66-67 -1.0922934524375307 2316 68-69 -1.1945656354116885 2316 70-71 -1.2062674932575739 2316 72-73 -1.106890076639253 2316 74-75 -1.1773997597750174 2316 76-77 -1.2734294343588815 2316 78-79 -1.1515994555473519 2316 80-81 -1.1532702265522552 2316 82-83 -1.2247650549588904 2316 84-85 -1.1561687179288072 2316 86-87 -1.1327620850766031 2316 88-89 -1.1188413414593477 2316 90-91 -1.167098487271879 2316 92-93 -1.1748299005489713 2316 94-95 -1.311596254870679 2316 96-97 -1.6077365806032269 2316 98-99 -1.5241496206399034 2316 100-101 -1.1874746973771408 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6570.0 3 1759.0 4 1715.0 5 1963.0 6 2356.0 7 2790.0 8 3326.0 9 4274.0 10 5363.0 11 6498.0 12 8297.0 13 10068.0 14 11756.0 15 14061.0 16 17099.0 17 22091.0 18 20031.0 19 16531.0 20 16327.0 21 17142.0 22 18396.0 23 20283.0 24 22423.0 25 25372.0 26 29157.0 27 33579.0 28 38738.0 29 45922.0 30 55342.0 31 67178.0 32 84037.0 33 109549.0 34 149168.0 35 217036.0 36 325311.0 37 451994.0 38 337858.0 39 37374.0 40 4.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.6163638280861 18.291201107686668 14.700073457209506 23.392361607017722 2 15.594538974609298 19.284567048695116 40.8630765973393 24.25781737935629 3 19.50994997437366 23.303312641780987 29.318569726898975 27.868167656946376 4 12.984567212103816 16.355140186915886 36.57144553248346 34.08884706849684 5 14.156725666500805 36.79402578493765 34.068959222366615 14.980289326194926 6 32.940581535627764 35.39641413250531 17.189631529821806 14.473372802045118 7 29.14358778510966 32.23523490616779 20.03492069961907 18.586256609103483 8 26.283418219768013 35.13673818153146 19.23136211941745 19.348481479283073 9 26.249686053047842 17.070652349652075 19.923616208211833 36.75604538908824 10-11 25.231595991407186 26.295308177686188 26.89987135586751 21.57322447503912 12-13 26.09802515470168 23.53918710371683 27.375793231153256 22.986994510428236 14-15 22.93276106508695 25.125213210749564 25.662700317327143 26.279325406836346 16-17 22.465579745857486 28.258331485669846 26.46439605990284 22.81169270856983 18-19 22.368487792625583 26.735730339368263 28.765157745542353 22.130624122463797 20-21 22.944261619593114 25.867278388404163 29.141156415490798 22.047303576511922 22-23 22.67706510052218 25.80399359755598 28.999257064861293 22.51968423706055 24-25 22.553438409985052 26.002494313130015 29.071531928781134 22.3725353481038 26-27 22.25926889913998 26.324969403633908 28.83496790842688 22.580793788799237 28-29 22.321420660250467 26.584571000228603 28.800033138342833 22.293975201178096 30-31 22.46737216685673 26.197365494643176 28.75688598135147 22.578376357148617 32-33 22.18479524494758 26.111076160231374 28.824913518724614 22.879215076096436 34-35 22.79144158762825 26.325812915152447 28.563728981392973 22.319016515826327 36-37 22.815781732347094 26.007047694657786 28.57025454635671 22.60691602663841 38-39 22.404622930801533 26.348460008543757 28.582770263910902 22.664146796743807 40-41 22.82192706112935 26.136498779184684 28.578594484461593 22.46297967522437 42-43 22.57753830685792 26.134013655157446 28.357771325941034 22.930676712043603 44-45 22.580393037301057 26.44799574534697 28.40875401060165 22.56285720675032 46-47 22.87580657339743 26.644705594759106 27.873422918032592 22.606064913810876 48-49 22.825951933826808 26.59480455582843 27.926447953776545 22.65279555656822 50-51 22.908473981804267 26.8425569679449 27.74324302780376 22.50572602244707 52-53 22.9419174501841 26.642618846178458 27.72583358999743 22.68963011364001 54-55 22.78287143523935 26.76352636189157 27.716011276507363 22.737590926361715 56-57 22.718762136599487 26.88396558420193 27.645142105326087 22.752130173872494 58-59 22.75947518993334 26.58232024112727 27.78547678897577 22.872727779963615 60-61 22.737013560713653 26.662295671474762 27.899260739384612 22.70143002842697 62-63 22.57101744679711 26.74346749751081 28.04725573675903 22.63825931893305 64-65 22.49305866150624 26.674157850687045 27.874666217933676 22.95811726987304 66-67 22.49211151130425 26.763430356799688 27.780077879730104 22.96438025216596 68-69 22.66259222079588 26.555249671988214 27.907858245771273 22.874299861444634 70-71 22.680547527776117 26.571219137340357 27.64457200309153 23.103661331792 72-73 22.686644372927123 26.67985305707757 27.595666472605874 23.037836097389437 74-75 22.66533184616437 26.974866711706895 27.354453524087774 23.005347918040957 76-77 22.827101137945252 26.741669113313165 27.438885870251124 22.992343878490455 78-79 22.780431224369114 26.73888203664258 27.541602212378898 22.93908452660941 80-81 22.8669597871183 26.84773153419752 27.27786981918384 23.007438859500336 82-83 22.943680770017632 26.857447013905556 27.30354781656817 22.89532439950864 84-85 22.97517415113017 26.938644203866474 27.277547849355592 22.808633795647765 86-87 22.978894105687097 26.797286359715848 27.213090365532054 23.010729169064998 88-89 22.937627447749744 26.837417862084482 27.070566412789866 23.154388277375908 90-91 22.977473217469495 26.879583262275002 27.082237927651175 23.06070559260432 92-93 23.031707343537594 26.59493879148982 27.174616587359 23.19873727761358 94-95 22.971703942772674 26.56604163058361 27.235735804242083 23.22651862240163 96-97 23.226273803043476 26.350937461106138 27.14991980943835 23.272868926412038 98-99 23.049679234156738 26.46852429111203 26.933524597805402 23.54827187692583 100-101 23.576946435805517 25.799480564633736 27.14285936020268 23.48071363935806 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 579.0 1 395.0 2 289.5 3 387.0 4 324.5 5 309.0 6 624.0 7 1409.0 8 2339.0 9 2714.0 10 2351.5 11 1963.0 12 2012.0 13 2120.0 14 2246.5 15 2372.0 16 2433.0 17 2596.5 18 2834.5 19 3087.0 20 3452.5 21 4169.5 22 5017.5 23 6014.0 24 7523.0 25 9665.0 26 12360.0 27 15314.5 28 18004.0 29 20979.5 30 24333.0 31 28086.0 32 32822.0 33 36011.0 34 38237.0 35 42090.0 36 47178.5 37 51704.0 38 55199.0 39 58123.5 40 61073.0 41 66050.5 42 69838.5 43 71384.5 44 74330.0 45 78645.0 46 81470.0 47 84124.0 48 85984.0 49 86310.0 50 86406.0 51 84601.5 52 84062.5 53 85394.0 54 85990.0 55 81359.0 56 72898.5 57 64749.0 58 58822.5 59 51949.0 60 42253.5 61 35212.5 62 29343.5 63 22560.5 64 16382.5 65 12206.5 66 9126.0 67 6856.5 68 5221.5 69 3832.5 70 2907.0 71 2095.0 72 1549.0 73 1297.0 74 993.5 75 701.0 76 512.0 77 317.5 78 196.0 79 111.0 80 64.5 81 44.0 82 26.5 83 22.5 84 17.5 85 11.0 86 8.0 87 6.5 88 8.0 89 7.5 90 6.5 91 4.5 92 1.5 93 1.5 94 3.0 95 3.0 96 3.0 97 2.0 98 0.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4945682057857086 2 0.050470661050551244 3 0.05733290005303847 4 0.06490349921062116 5 0.08141714532628397 6 0.10784783361328318 7 0.07189855574218879 8 0.053658281748480786 9 0.05272855904491801 10-11 0.0768792130827037 12-13 0.10891037384592635 14-15 0.05704512874003094 16-17 0.06120674465121674 18-19 0.05987856936041276 20-21 0.07617085292760825 22-23 0.06530195179786234 24-25 0.08874424568055259 26-27 0.06753771353738239 28-29 0.06791402986977683 30-31 0.07154437566464106 32-33 0.07092456052893252 34-35 0.0735145023460003 36-37 0.06844529998609844 38-39 0.04203674795394596 40-41 0.045401458690649384 42-43 0.04351987702867707 44-45 0.022335481140353594 46-47 0.028843540065293098 48-49 0.02937481018161469 50-51 0.027714591068109713 52-53 0.026497097051539402 54-55 0.02620932573853187 56-57 0.026076508209451472 58-59 0.02264538870820786 60-61 0.029706854004315685 62-63 0.020918760830162683 64-65 0.023375885118150044 66-67 0.01700064372229094 68-69 0.01925854171665771 70-71 0.042191701737873094 72-73 0.03783085953306669 74-75 0.03349215358310703 76-77 0.02412851778293897 78-79 0.015694604686333696 80-81 0.017465505074072334 82-83 0.02231334488550686 84-85 0.01888222538426325 86-87 0.009828497151949452 88-89 0.042058884208792696 90-91 0.05325982916123959 92-93 0.03362497111218743 94-95 0.05379109927756119 96-97 0.0438740571062248 98-99 0.03464323883513715 100-101 0.1677706754833894 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2258738.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.90410672696472 #Duplication Level Percentage of deduplicated Percentage of total 1 76.49009457293879 42.76110410561195 2 13.229505889763447 14.79167418412688 3 4.334093973508129 7.268809561790793 4 1.9390826567758601 4.336107351872159 5 1.0434121214154697 2.9165511297909545 6 0.6451782115619643 2.1640866958243365 7 0.42321878648945893 1.6561767746134244 8 0.29665461765882745 1.3267369125316804 9 0.2231200260356325 1.1225993173577233 >10 1.2553949467484922 12.898513631746578 >50 0.083369704126864 3.1796797118420232 >100 0.03346799154094047 3.273446403849801 >500 0.002376628908912274 0.8545768496325626 >1k 8.714305999343414E-4 0.767241363133931 >5k 1.5844192726078936E-4 0.6826960062750835 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8494 0.3760506973363002 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6921 0.3064100395884782 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4346 0.19240832712780323 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 8.8545019386932E-5 0.0 7 0.0 0.0 0.0 1.3281752908039798E-4 0.0 8 0.0 0.0 0.0 1.3281752908039798E-4 0.0 9 0.0 0.0 0.0 1.3281752908039798E-4 0.0 10-11 0.0 0.0 0.0 1.3281752908039798E-4 0.0 12-13 0.0 0.0 0.0 1.3281752908039798E-4 0.0 14-15 0.0 0.0 0.0 1.3281752908039798E-4 0.0 16-17 0.0 0.0 0.0 1.3281752908039798E-4 0.0 18-19 0.0 0.0 0.0 1.99226293620597E-4 0.0 20-21 0.0 0.0 0.0 2.4349880331406298E-4 0.0 22-23 0.0 0.0 0.0 3.5418007754772795E-4 0.0 24-25 0.0 0.0 0.0 6.640876454019899E-4 0.0 26-27 0.0 0.0 0.0 0.0010846764874899168 0.0 28-29 0.0 0.0 0.0 0.003342574481856683 0.0 30-31 0.0 0.0 0.0 0.009872769661642918 0.0 32-33 0.0 0.0 0.0 0.025146785505888686 0.0 34-35 0.0 0.0 0.0 0.049872982169689446 0.0 36-37 0.0 0.0 0.0 0.0899617396971229 0.0 38-39 0.0 0.0 0.0 0.1484678612570382 0.0 40-41 0.0 0.0 0.0 0.21013946726003635 0.0 42-43 0.0 0.0 0.0 0.2764596867808484 0.0 44-45 0.0 0.0 0.0 0.35026196043985625 0.0 46-47 0.0 0.0 0.0 0.4371467607132833 0.0 48-49 0.0 0.0 0.0 0.5323105202993884 0.0 50-51 0.0 0.0 0.0 0.6303077205058754 0.0 52-53 0.0 0.0 0.0 0.7285262832608297 0.0 54-55 0.0 0.0 0.0 0.8320575471789999 0.0 56-57 0.0 0.0 0.0 0.9517925496449788 0.0 58-59 0.0 0.0 0.0 1.0833040396894194 0.0 60-61 0.0 0.0 0.0 1.2190435544095863 0.0 62-63 0.0 0.0 0.0 1.3550044316782204 0.0 64-65 0.0 0.0 0.0 1.4964329638939975 0.0 66-67 0.0 0.0 0.0 1.653910280873656 0.0 68-69 0.0 0.0 0.0 1.8285210591046859 0.0 70-71 0.0 0.0 0.0 2.012584018155271 0.0 72-73 0.0 0.0 0.0 2.1916663198653406 0.0 74-75 4.4272509693466E-5 0.0 0.0 2.378319220732993 0.0 76-77 4.4272509693466E-5 0.0 0.0 2.5793606872510226 0.0 78-79 4.4272509693466E-5 0.0 0.0 2.80433144525837 0.0 80-81 4.4272509693466E-5 0.0 0.0 3.0357881259358104 0.0 82-83 4.4272509693466E-5 0.0 0.0 3.2788884766626323 0.0 84-85 4.4272509693466E-5 0.0 0.0 3.5294708815276494 0.0 86-87 4.4272509693466E-5 0.0 0.0 3.789084878370134 0.0 88-89 4.4272509693466E-5 0.0 0.0 4.060940224142862 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4140 0.0 52.822906 1 GTATCAA 7200 0.0 43.106033 1 TATCAAC 9335 0.0 33.089592 2 ATCAACG 9335 0.0 33.089592 3 TCAACGC 9435 0.0 32.63814 4 CAACGCA 9585 0.0 32.077797 5 AACGCAG 9935 0.0 31.043394 6 TGGTATC 1875 0.0 27.625973 2 ACGCAGA 11405 0.0 26.969011 7 CGCAGAG 11500 0.0 26.58087 8 GTGGTAT 2155 0.0 25.037348 1 GCAGAGT 12670 0.0 23.450375 9 GAGTACT 7870 0.0 22.043608 12-13 GTACATG 7335 0.0 21.937489 1 TACATGG 7395 0.0 21.33498 2 AGAGTAC 11325 0.0 21.239498 10-11 GTACTTT 8200 0.0 20.884773 14-15 CAGAGTA 12110 0.0 20.84406 10-11 AGTACTT 8240 0.0 20.41929 12-13 ACATGGG 7850 0.0 19.069237 3 >>END_MODULE